CN112501039B - Methodactis bijianensis and method for treating high-salt wastewater - Google Patents
Methodactis bijianensis and method for treating high-salt wastewater Download PDFInfo
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- CN112501039B CN112501039B CN202011250992.5A CN202011250992A CN112501039B CN 112501039 B CN112501039 B CN 112501039B CN 202011250992 A CN202011250992 A CN 202011250992A CN 112501039 B CN112501039 B CN 112501039B
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- 239000002351 wastewater Substances 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 241000512259 Ascophyllum nodosum Species 0.000 claims abstract description 20
- 241001261506 Undaria pinnatifida Species 0.000 claims abstract description 19
- 238000004321 preservation Methods 0.000 claims abstract description 19
- 230000001580 bacterial effect Effects 0.000 claims abstract description 14
- 238000009629 microbiological culture Methods 0.000 claims abstract description 12
- 208000020584 Polyploidy Diseases 0.000 claims abstract description 3
- 239000002131 composite material Substances 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 241000206596 Halomonas Species 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 241000235058 Komagataella pastoris Species 0.000 claims description 3
- 235000017788 Cydonia oblonga Nutrition 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract description 3
- 239000002028 Biomass Substances 0.000 description 3
- 241000235048 Meyerozyma guilliermondii Species 0.000 description 2
- 206010033546 Pallor Diseases 0.000 description 2
- 210000004763 bicuspid Anatomy 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001123674 Metschnikowia Species 0.000 description 1
- 241000311506 Meyerozyma Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- PVBQYTCFVWZSJK-UHFFFAOYSA-N meldonium Chemical compound C[N+](C)(C)NCCC([O-])=O PVBQYTCFVWZSJK-UHFFFAOYSA-N 0.000 description 1
- 229960002937 meldonium Drugs 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 238000009938 salting Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000003902 seawater pollution Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
Abstract
The invention discloses a two-tip Meiqi microzymeMetschnikowia bicuspidate），Has been preserved in China general microbiological culture Collection center with a preservation number of CGMCC No.18674. The method for treating the high-salt wastewater by using the quincuncial microzyme comprises the following steps: gradually expanding and culturing the seed liquid of the bicuspension mermaid microzyme; and adding the bacterial liquid with the expanded culture polyploidy volume into the high-salt wastewater for culture. The method is simple to operate and low in cost, the COD removal rate of the high-salt wastewater produced by kelp processing is 82.92%, the COD removal rate of the high-salt wastewater produced by undaria pinnatifida processing is 85.60%, and the method meets the urban pipe network emission standard.
Description
Technical Field
The invention relates to a bacterium for treating wastewater and a treatment method, in particular to a bicuspid merocmyces (Latin academic name)Metschnikowia bicuspidate，Preservation date: 2019-10-12; preservation unit: china general microbiological culture Collection center (China Committee for culture Collection); deposit number: CGMCC No. 18674) and a method for treating high-salt wastewater.
Background
In order to prevent fresh kelp and undaria pinnatifida from spoiling and deteriorating, the collected fresh kelp is usually subjected to blanching, cooling and salting treatment immediately so as to realize sterilization and dehydration. The blanching water is seawater which is repeatedly heated to about 95 ℃, and is recycled for 8-10 hours to obtain high-salt wastewater, wherein the content of organic matters (phycocolloids, mannitol and the like) and inorganic matters is high, the COD value can reach 1200-2100, and the high-salt wastewater can cause seawater pollution if directly discharged, so that the high-salt wastewater can be discharged to a city pipe network after being treated to reach standards according to regulations. At present, related reports on the treatment of industrial wastewater by using biotechnology are presented, and the method has the characteristics of simple operation, low price and the like, but is limited to the treatment of fresh water wastewater. Although the MVR high-efficiency evaporation crystallization system can be used for treating high-salt wastewater produced by kelp and undaria pinnatifida processing, the cost is high (100-120 yuan/ton), and the production benefit of a processing plant is directly affected.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provides a two-point merzizyphus and a method for treating high-salt wastewater.
The technical scheme of the invention is as follows: double-tip plum blossom microzymeMetschnikowia bicuspidate），Has been preserved in China general microbiological culture Collection center with a preservation number of CGMCC No.18674.
The method for treating the high-salt wastewater by using the meldonium oxysporum comprises the following steps of:
a. gradually expanding and culturing the seed liquid of the bicuspension Meiqi microzyme;
b. and adding the bacterial liquid with the expanded culture polyploidy volume into the high-salt wastewater for culture.
The step a is preferably to combine the above two-point Meiqi yeast with Pichia pastoris with collection number of CGMCC No.18675 (Latin academic name)Meyerozyma guilliermondiiPreservation date: 2019-10-12; preservation unit: china general microbiological culture Collection center (China Committee for culture Collection); deposit number: CGMCC No. 18675) according to the volume ratio of 1: and 1, preparing composite seed liquid for gradual expansion culture, wherein in the step b, bacterial liquid with the volume which is 8-10 times of that of the expansion culture is added into high-salt wastewater in an addition amount of 1-3% by volume, and shake culture is carried out for 60 hours at the temperature of 10-28 ℃ at the speed of 100-150 r/min.
The step a is preferably to combine the above two-point Meiqi microzyme with halomonas (Latin academic name) with the preservation number of CGMCC No.20812Cobetia sp.Preservation date: 2020-9-23; preservation unit: china general microbiological culture Collection center (China Committee for culture Collection); deposit number: CGMCC No. 20812) according to a volume ratio of 2:1, preparing composite seed liquid and gradually expanding culture; and the step b is preferably to add 20-50 times of bacterial liquid by volume of expansion culture into the high-salt wastewater with the addition amount of 1-3% by volume percent, and shake culture for 60 hours at the temperature of 20-28 ℃ at the speed of 100-150 r/min.
The invention separates, purifies, identifies, optimizes and screens out the bicuspid plum saccharomycete (CGMCC No. 18674) which can utilize the organic matters of the high-salt wastewater, and the screened strain is used for treating the high-salt wastewater produced by kelp or undaria pinnatifida processing, so that the invention has simple operation and low cost, the COD removal rate of the high-salt wastewater produced by kelp processing is 82.92%, the COD removal rate of the high-salt wastewater produced by undaria pinnatifida processing is 85.60%, and the invention reaches the urban pipe network emission standard. Especially, the kelp or the undaria pinnatifida is processed to produce high-salt wastewater by matching the two-point Meissima yeast with the Pichia guilliermondii (CGMCC No. 18675) or the halomonas (CGMCC No. 20812), the COD removal rate of the high-salt wastewater produced by the kelp processing reaches 85.98 percent, and the COD removal rate of the high-salt wastewater produced by the undaria pinnatifida processing is 86.24 percent.
Drawings
FIGS. 1 and 2 are graphs showing the COD removal rate and the biomass change with time of cultivation in example 1 of the present invention.
FIG. 3 is a graph showing the change of the COD removal rate and biomass with time of cultivation in example 2 of the present invention.
FIG. 4 is a graph showing the change of the COD removal rate and biomass with time of cultivation in example 3 of the present invention.
Date of collection of the mycelial bijiangensis strain: 2019-10-12;
preservation unit: china general microbiological culture Collection center (address: north Chen Xi Lu 1, korea, beijing, city);
deposit number: CGMCC No.18674.
Pichia guilliermondii strain preservation date: 2019-10-12;
preservation unit: china general microbiological culture Collection center (address: north Chen Xi Lu 1, korea, beijing, city);
deposit number: CGMCC No.18675.
The preservation date of the halomonas strain: 2020-9-23;
preservation unit: china general microbiological culture Collection center (address: north Chen Xi Lu 1, korea, beijing, city);
deposit number: CGMCC No.20812.
Detailed Description
Example 1:
the invention separates, purifies, identifies, optimizes and screens out the strain which can utilize the high-salt wastewater organic matters as the Meilinchus bicuspension microzymeMetschnikowia bicuspidate），The strain is preserved in China general microbiological culture Collection center, with the code of WSKeW-M and the preservation number of CGMCC No.18674.
The method for treating the high-salt wastewater by using the bipointed merzizyweed comprises the following steps:
a. gradually expanding and culturing seed solution of the above two-point Meiqijijun microzyme (CGMCC No. 18674);
b. the bacterial liquid which is 8 times of the volume of the expansion culture is respectively added into the wastewater which is processed by kelp and is produced by undaria pinnatifida and is produced by high salt in the adding amount of 5 percent by volume, and the bacterial liquid is cultivated by a shaking table at 120 r/min for 60 hours at 20 ℃.
The high-salt wastewater produced by processing kelp and the high-salt wastewater produced by processing undaria pinnatifida treated by the method are measured, and the results are respectively shown in figures 1 and 2. The result shows that the COD removal rate of the high-salt wastewater produced by kelp processing is 82.92 percent, and the COD removal rate of the high-salt wastewater produced by undaria pinnatifida processing is 85.60 percent.
Example 2:
the method for treating the high-salt wastewater by using the bipointed merzizyweed comprises the following steps:
a. mixing the above two-point Meiqi yeast (CGMCC No. 18674) with Pichia pastoris with collection number of CGMCC No.18675Meyerozyma guilliermondii) The volume ratio is 1:1, preparing composite seed liquid and gradually expanding culture;
b. the bacterial liquid which is expanded and cultivated by 8 times of volume is added into the wastewater which is produced by processing kelp and is high in salt content by 3 percent of volume percent, and the bacterial liquid is cultivated by a shaking table at 150 r/min for 60 hours at 20 ℃.
The high-salt wastewater produced by kelp processing treated by the method is measured, and the result is shown in figure 3. The result shows that the COD removal rate of the wastewater with high salt content produced by kelp processing is 85.98%.
Example 3:
the method for treating the high-salt wastewater by using the bipointed merzizyweed comprises the following steps:
a. mixing the above two-point Meiqi microzyme (CGMCC No. 18674) with Salmonella having a preservation number of CGMCC No.20812Cobetia sp.) The volume ratio is 2:1, preparing composite seed liquid and gradually expanding culture;
b. the bacterial liquid which is cultivated for 20 times of volume is added into the wastewater which is processed by undaria pinnatifida and produces high salt by adding the bacterial liquid with the volume percentage of 1 percent, and the bacterial liquid is cultivated for 60 hours by shaking at 150 r/min under the condition of 20 ℃.
COD measurement is carried out on the wastewater with high salt produced by processing the undaria pinnatifida treated by the method by using a potassium permanganate oxidation method, and the result is shown in figure 4. The result shows that the COD removal rate of the wastewater produced by processing the undaria pinnatifida is 86.24%.
Claims (4)
1. Double-tip plum blossom microzymeMetschnikowia bicuspidata），Has been preserved in China general microbiological culture Collection center with a preservation number of CGMCC No.18674.
2. A method for treating kelp or undaria pinnatifida processing high-salt wastewater by using the quince yeast according to claim 1, which is characterized by comprising the following steps:
a. gradually expanding the seed liquid of the Mei-Qihai microzyme according to claim 1;
b. adding the bacterial liquid with the expanded culture polyploidy volume into the wastewater produced by processing kelp or undaria pinnatifida to culture.
3. The method for treating high-salinity wastewater produced by kelp or undaria pinnatifida processing according to claim 2, wherein the step a is characterized in that the Meissima spinosa of claim 1 is mixed with Pichia pastoris with the preservation number of CGMCC No.18675 and preserved in China general microbiological culture Collection centerMeyerozyma guilliermondii) The volume ratio is 1: and (2) preparing composite seed liquid for gradual expansion culture, wherein in the step (b), bacterial liquid with the volume which is 8-10 times of that of the expansion culture is added into wastewater which is produced by processing kelp or undaria pinnatifida and is high in salt content in an adding amount of 1-3% by volume, and shake culture is carried out for 60 hours at the temperature of 10-28 ℃ at the speed of 100-150 r/min.
4. The method for processing and producing high-salinity wastewater by kelp or undaria pinnatifida according to claim 2, wherein the step a is characterized in that the two-point Mei-qi microzyme according to claim 1 is mixed with halomonas with the preservation number of CGMCC No.20812 preserved in the China general microbiological culture Collection centerCobetia) The volume ratio is 2:1, preparing composite seed liquid and gradually expanding culture; the step b is toThe bacterial liquid with the volume being 20-50 times of that of the expansion culture is added into the wastewater produced by processing kelp or undaria pinnatifida into high-salt wastewater with the volume percentage being 1-3 percent, and the culture is carried out for 60 hours by a shaking table at the temperature of 20-28 ℃ and the speed of 100-150 r/min.
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| CN202011250992.5A CN112501039B (en) | 2020-11-11 | 2020-11-11 | Methodactis bijianensis and method for treating high-salt wastewater |
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| CN112501039B true CN112501039B (en) | 2024-02-02 |
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| CN114250317B (en) * | 2022-01-24 | 2022-09-16 | 天津市水产研究所 | Primer group and kit for detecting Erjiangmeiqi yeast |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201422812A (en) * | 2012-12-07 | 2014-06-16 | Univ Soochow | Metschnikowia bicuspidate strain, composition containing the strain and use of the strain |
| CN110382684A (en) * | 2016-12-21 | 2019-10-25 | 创新生物科学公司 | Generate the plum surprise yeast kind of xylitol |
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2020
- 2020-11-11 CN CN202011250992.5A patent/CN112501039B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201422812A (en) * | 2012-12-07 | 2014-06-16 | Univ Soochow | Metschnikowia bicuspidate strain, composition containing the strain and use of the strain |
| CN110382684A (en) * | 2016-12-21 | 2019-10-25 | 创新生物科学公司 | Generate the plum surprise yeast kind of xylitol |
Non-Patent Citations (2)
| Title |
|---|
| 杨婷婷.甘露醇利用菌的筛选及在褐藻加工废水中的应用.《中国优秀硕士学位论文全文数据库 基础科学辑》.2020,(第03期),第2.3节，2.4节，第3章和第4章. * |
| 池振明 ; 居靓 ; 王祥红 ; 池哲 ; 刘光磊 ; .在海洋环境中的酵母菌分布与多样性.中国海洋大学学报(自然科学版).2009,(第05期),全文. * |
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