CN112501039A - Saccharomycetes dicephalus and method for treating high-salinity wastewater - Google Patents
Saccharomycetes dicephalus and method for treating high-salinity wastewater Download PDFInfo
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- CN112501039A CN112501039A CN202011250992.5A CN202011250992A CN112501039A CN 112501039 A CN112501039 A CN 112501039A CN 202011250992 A CN202011250992 A CN 202011250992A CN 112501039 A CN112501039 A CN 112501039A
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- 239000002351 wastewater Substances 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 20
- 241000235342 Saccharomycetes Species 0.000 title claims description 4
- 238000004321 preservation Methods 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 238000009629 microbiological culture Methods 0.000 claims abstract description 11
- 230000001580 bacterial effect Effects 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 241000235048 Meyerozyma guilliermondii Species 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 6
- 241000206596 Halomonas Species 0.000 claims description 5
- 230000003321 amplification Effects 0.000 claims description 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 4
- 241000512259 Ascophyllum nodosum Species 0.000 abstract description 14
- 241001261506 Undaria pinnatifida Species 0.000 abstract description 14
- 241000233866 Fungi Species 0.000 abstract description 5
- 241001123674 Metschnikowia Species 0.000 abstract description 3
- 241000209051 Saccharum Species 0.000 abstract 1
- 239000002028 Biomass Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 206010033546 Pallor Diseases 0.000 description 2
- 241001326564 Saccharomycotina Species 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000086967 Cobetia sp. Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241000653900 Micrantha Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000004763 bicuspid Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000009938 salting Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000003902 seawater pollution Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
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Abstract
The invention discloses a yeast (Saccharum incarnatum) with two tipsMetschnikowia bicuspidate），Has been preserved in the China general microbiological culture Collection center with the preservation number of CGMCC No. 18674. The method for treating high-salinity wastewater by using the bicinchondric fungus comprises the following steps: gradually expanding and culturing the two-tip Metschnikowia yeast seed liquid; adding the bacterial liquid with multiple volumes of expanded culture into high-salinity wastewater for culture. Not only has simple operation and low cost, but alsoThe removal rate of COD in the high-salt wastewater produced by processing the kelp is 82.92%, the removal rate of COD in the high-salt wastewater produced by processing the undaria pinnatifida is 85.60%, and the urban pipe network discharge standard is reached.
Description
Technical Field
The invention relates to a bacterium for wastewater treatment and a treatment method, in particular to a bicuspid metschomyces (Latin literature name)Metschnikowia bicuspidate，The preservation date is as follows: 2019-10-12; the preservation unit: china general microbiological culture Collection center; the preservation number is as follows: CGMCC No. 18674) and a method for treating high-salinity wastewater.
Background
In order to prevent the fresh kelp and undaria pinnatifida from being deteriorated, the fresh picked kelp and undaria pinnatifida are generally subjected to blanching, cooling and salting treatments immediately to realize sterilization and dehydration. The blanching water is seawater repeatedly heated to about 95 ℃, and the high-salinity wastewater is obtained after 8-10 hours of recycling, wherein the contents of organic matters (phycocolloid, mannitol and the like) and inorganic matters are high, the COD value can reach 1200-2100, and the high-salinity wastewater causes seawater pollution if being directly discharged, so the high-salinity wastewater can be discharged to an urban pipe network after being treated to reach the standard according to the regulations. At present, related reports of the utilization of biotechnology for treating industrial wastewater have the characteristics of simple operation, low price and the like, but the method is only limited to fresh water wastewater treatment. Although the MVR efficient evaporative crystallization system can be used for treating high-salt wastewater produced by processing kelp and undaria pinnatifida, the cost is high (100-120 yuan/ton), and the production benefit of a processing plant is directly influenced.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provides a bicinchonia micrantha and a method for treating high-salinity wastewater.
The technical solution of the invention is as follows: a yeast, Erjiangmeiqi yeastMetschnikowia bicuspidate），Has been preserved in China general culture Collection of microorganismsThe preservation number of the microorganism-passing center is CGMCC No. 18674.
A method for treating high-salinity wastewater by using the bicinchondric fungus comprises the following steps:
a. gradually enlarging and culturing the seed liquid of the Erjiangqijimaozi;
b. adding the bacterial liquid with multiple volumes of expanded culture into high-salinity wastewater for culture.
The step a is preferably carried out by mixing the above bicinchi yeast and Pichia guilliermondii (Latin literature name) with preservation number of CGMCC No.18675Meyerozyma guilliermondiiThe preservation date: 2019-10-12; the preservation unit: china general microbiological culture Collection center; the preservation number is as follows: CGMCC No. 18675) according to the volume ratio of 1: 1, gradually carrying out amplification culture on the prepared composite seed solution, and preferably, adding bacterial solution with 8-10 times volume of the amplification culture into high-salinity wastewater in an adding amount of 1-3% by volume percentage, and carrying out shake culture at 10-28 ℃ for 60 hours at 100-150 r/min.
The preferable step a is to mix the above-mentioned bicinchons bicinchonii and halomonas (Latin literature name) with preservation number of CGMCC No.20812Cobetia sp.The preservation date: 2020-9-23; the preservation unit: china general microbiological culture Collection center; the preservation number is as follows: CGMCC No. 20812) according to the volume ratio of 2: 1 preparing a composite seed solution for gradually expanding culture; preferably, the bacterial liquid with 20-50 times volume of expanded culture is added into the high-salinity wastewater in an adding amount of 1-3% by volume, and is subjected to shake culture at the temperature of 20-28 ℃ for 60 hours at a speed of 100-150 r/min.
The method screens out the bicinchi Meiqijie (CGMCC No. 18674) which can utilize organic substances in the high-salt wastewater through separation, purification, identification and optimization, and treats the high-salt wastewater produced by processing the kelp or the undaria pinnatifida by using the screened strains, so that the method is simple to operate and low in cost, the removal rate of COD (chemical oxygen demand) in the high-salt wastewater produced by processing the kelp is 82.92%, the removal rate of COD in the high-salt wastewater produced by processing the undaria pinnatifida is 85.60%, and the urban pipe network discharge standard is met. Particularly, the bicinchi sychia microzyme and the pichia guilliermondii (CGMCC No. 18675) or the halomonas (CGMCC No. 20812) are matched to treat the high-salt wastewater produced by processing the kelp or the undaria pinnatifida, the removal rate of the COD of the high-salt wastewater produced by processing the kelp reaches 85.98 percent, and the removal rate of the COD of the high-salt wastewater produced by processing the undaria pinnatifida reaches 86.24 percent.
Drawings
FIGS. 1 and 2 are graphs showing the COD removal rate and biomass change with culture time in example 1 of the present invention.
FIG. 3 is a graph showing the COD removal rate and biomass change with culture time in example 2 of the present invention.
FIG. 4 is a graph showing the COD removal rate and biomass change with culture time in example 3 of the present invention.
Date of deposit of the bicinchi yeast strain: 2019-10-12;
the preservation unit: china general microbiological culture Collection center (address: West Lu No.1 Hospital, Chaozhou, Chaoyang, China);
the preservation number is as follows: CGMCC No. 18674.
The date of deposit of the pichia guilliermondii strain: 2019-10-12;
the preservation unit: china general microbiological culture Collection center (address: West Lu No.1 Hospital, Chaozhou, Chaoyang, China);
the preservation number is as follows: CGMCC No. 18675.
The preservation date of the halomonas strain: 2020-9-23;
the preservation unit: china general microbiological culture Collection center (address: West Lu No.1 Hospital, Chaozhou, Chaoyang, China);
the preservation number is as follows: CGMCC No. 20812.
Detailed Description
Example 1:
the strain capable of utilizing the organic substances of the high-salinity wastewater is screened out by separation, purification, identification and optimization, and is Erjiangmei saccharomycete (a yeast)Metschnikowia bicuspidate），Has been preserved in the China general microbiological culture Collection center with the code number of WSKeW-M and the preservation number of CGMCC No. 18674.
The method for treating high-salinity wastewater by using the bicinchondric fungus comprises the following steps:
a. gradually enlarging and culturing the seed solution of the above Saccharomycotina bicolor (CGMCC No. 18674);
b. adding the bacterial liquid with 8 times of volume of expanded culture into the high-salt wastewater produced by processing kelp and the high-salt wastewater produced by processing undaria pinnatifida respectively by the addition amount of 5 percent by volume, and culturing for 60 hours at 20 ℃ in a shaking table at 120 r/min.
The results of the measurement of the high-salt wastewater from the kelp processing and the high-salt wastewater from the undaria pinnatifida processing, which are treated by the above method, are shown in fig. 1 and 2, respectively. The result shows that the removal rate of the COD of the high-salt wastewater produced by the kelp processing is 82.92%, and the removal rate of the COD of the high-salt wastewater produced by the undaria pinnatifida processing is 85.60%.
Example 2:
the method for treating high-salinity wastewater by using the bicinchondric fungus comprises the following steps:
a. mixing the above bicinchi yeast (CGMCC No. 18674) and Pichia guilliermondii (CGMCC No. 18675)Meyerozyma guilliermondii) According to the volume ratio of 1: 1 preparing a composite seed solution for gradually expanding culture;
b. adding the bacterial liquid with 8 times of volume of expanded culture into the high-salinity wastewater produced by processing kelp by the addition amount of 3 percent in volume percentage, and culturing for 60 hours at 20 ℃ by a shaking table at 150 r/min.
The results of the determination of the high-salinity wastewater produced by the kelp processing treated by the method are shown in fig. 3. The result shows that the removal rate of COD in the high-salinity wastewater produced by kelp processing is 85.98%.
Example 3:
the method for treating high-salinity wastewater by using the bicinchondric fungus comprises the following steps:
a. mixing the above Saccharomycotina bicinchi (CGMCC No. 18674) and Halomonas with preservation number CGMCC No.20812 (see below)Cobetia sp.) According to the volume ratio of 2: 1 preparing a composite seed solution for gradually expanding culture;
b. adding the bacterial liquid with 20 times volume of expanded culture into the high-salt wastewater produced by processing the undaria pinnatifida in an adding amount of 1 percent by volume, and culturing for 60 hours at 20 ℃ by a shaking table at 150 r/min.
COD measurement of the high-salt wastewater from the processing of undaria pinnatifida treated by the above method by potassium permanganate oxidation method is shown in FIG. 4. The result shows that the COD removal rate of the high-salinity wastewater produced by processing the undaria pinnatifida is 86.24%.
Claims (4)
1. A yeast, Erjiangmeiqi yeastMetschnikowia bicuspidate），Has been preserved in the China general microbiological culture Collection center with the preservation number of CGMCC No. 18674.
2. A method for treating high-salinity wastewater by using the Erichthyophthirius multifiliis disclosed in claim 1, which is characterized by comprising the following steps:
a. gradually expanding and culturing the seed liquid of the Ericaria distachya of claim 1;
b. adding the bacterial liquid with multiple volumes of expanded culture into high-salinity wastewater for culture.
3. The method for treating high-salinity wastewater according to claim 2, wherein the step a comprises the step of collecting the bicinchi yeast of claim 1 and the pichia guilliermondii (CGMCC No. 18675) deposited in the china general microbiological culture collection center with the collection number of CGMCC No.18675Meyerozyma guilliermondii) According to the volume ratio of 1: and 1, preparing a composite seed solution, gradually carrying out amplification culture, adding a bacterial solution with 8-10 times volume of amplification culture into the high-salinity wastewater in an adding amount of 1-3% by volume percentage, and carrying out shake culture at 10-28 ℃ for 60 hours at 100-150 r/min.
4. The method for treating high-salinity wastewater according to claim 2, wherein the step a comprises the Saccharomycetes bicuspidata as claimed in claim 1 and the halomonas (CGMCC No. 20812) deposited in the China general microbiological culture Collection center with the preservation number of CGMCC No.20812Cobetia sp.) According to the volume ratio of 2: 1 preparing a composite seed solution for gradually expanding culture; step b is to enlarge and culture 20-50 times of volumeAdding the bacterial liquid into the high-salinity wastewater in an adding amount of 1-3% by volume, and performing shake cultivation for 60 hours at the temperature of 20-28 ℃ at a speed of 100-150 r/min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114250317A (en) * | 2022-01-24 | 2022-03-29 | 天津市水产研究所 | Primer group and kit for detecting Erjiangmeiqi yeast |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201422812A (en) * | 2012-12-07 | 2014-06-16 | Univ Soochow | Metschnikowia bicuspidate strain, composition containing the strain and use of the strain |
| CN110382684A (en) * | 2016-12-21 | 2019-10-25 | 创新生物科学公司 | Generate the plum surprise yeast kind of xylitol |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201422812A (en) * | 2012-12-07 | 2014-06-16 | Univ Soochow | Metschnikowia bicuspidate strain, composition containing the strain and use of the strain |
| CN110382684A (en) * | 2016-12-21 | 2019-10-25 | 创新生物科学公司 | Generate the plum surprise yeast kind of xylitol |
Non-Patent Citations (2)
| Title |
|---|
| 杨婷婷: "甘露醇利用菌的筛选及在褐藻加工废水中的应用", no. 03, pages 2 * |
| 池振明;居靓;王祥红;池哲;刘光磊;: "在海洋环境中的酵母菌分布与多样性", no. 05 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114250317A (en) * | 2022-01-24 | 2022-03-29 | 天津市水产研究所 | Primer group and kit for detecting Erjiangmeiqi yeast |
| CN114250317B (en) * | 2022-01-24 | 2022-09-16 | 天津市水产研究所 | Primer group and kit for detecting Erjiangmeiqi yeast |
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