CN112501057B - Halomonas and method for treating high-salt wastewater - Google Patents
Halomonas and method for treating high-salt wastewater Download PDFInfo
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- CN112501057B CN112501057B CN202011252442.7A CN202011252442A CN112501057B CN 112501057 B CN112501057 B CN 112501057B CN 202011252442 A CN202011252442 A CN 202011252442A CN 112501057 B CN112501057 B CN 112501057B
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- halomonas
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- wastewater
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- 239000002351 wastewater Substances 0.000 title claims abstract description 35
- 241000206596 Halomonas Species 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241001261506 Undaria pinnatifida Species 0.000 claims abstract description 14
- 241000512259 Ascophyllum nodosum Species 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 230000001580 bacterial effect Effects 0.000 claims abstract description 9
- 238000009629 microbiological culture Methods 0.000 claims abstract description 7
- 208000020584 Polyploidy Diseases 0.000 claims abstract description 3
- 241000607142 Salmonella Species 0.000 claims description 2
- 238000004321 preservation Methods 0.000 abstract description 12
- 238000012258 culturing Methods 0.000 abstract description 3
- 150000003839 salts Chemical class 0.000 description 6
- 239000002028 Biomass Substances 0.000 description 2
- 206010033546 Pallor Diseases 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000086967 Cobetia sp. Species 0.000 description 1
- 235000017788 Cydonia oblonga Nutrition 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001123674 Metschnikowia Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000009938 salting Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000003902 seawater pollution Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
Abstract
The invention discloses a halomonas spCobetia sp.) Has been preserved in China general microbiological culture Collection center with a preservation number of CGMCC No.20812. The method for treating the high-salt wastewater by using the halomonas comprises the following steps of: gradually expanding and culturing the halomonas seed solution; and adding the bacterial liquid with the expanded culture polyploidy volume into the high-salt wastewater for culture. The method is simple to operate and low in cost, the COD removal rate of the high-salt wastewater produced by kelp processing is 84.82%, the COD removal rate of the high-salt wastewater produced by undaria pinnatifida processing is 85.87%, and the method meets the urban pipe network emission standard.
Description
Technical Field
The invention relates to a bacterium for wastewater treatment and a treatment method, in particular to a halomonas (Latin academic name)Cobetia sp.Preservation date: 2020-9-23; preservation unit: china general microbiological culture Collection center (China Committee for culture Collection); deposit number: CGMCC No. 20812) and a method for treating high-salt wastewater.
Background
In order to prevent fresh kelp and undaria pinnatifida from spoiling and deteriorating, the collected fresh kelp is usually subjected to blanching, cooling and salting treatment immediately so as to realize sterilization and dehydration. The blanching water is seawater which is repeatedly heated to about 95 ℃, and is recycled for 8-10 hours to obtain high-salt wastewater, wherein the content of organic matters (phycocolloids, mannitol and the like) and inorganic matters is high, the COD value can reach 1200-2100, and the high-salt wastewater can cause seawater pollution if directly discharged, so that the high-salt wastewater can be discharged to a city pipe network after being treated to reach standards according to regulations. At present, related reports on the treatment of industrial wastewater by using biotechnology are presented, and the method has the characteristics of simple operation, low price and the like, but is limited to the treatment of fresh water wastewater. Although the MVR high-efficiency evaporation crystallization system can be used for treating high-salt wastewater produced by kelp and undaria pinnatifida processing, the cost is high (100-120 yuan/ton), and the production benefit of a processing plant is directly affected.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provides halomonas and a method for treating high-salt wastewater.
The technical scheme of the invention is as follows: salmonella spCobetia sp.) Has been preserved in China general microbiological culture Collection center with a preservation number of CGMCC No.20812.
The method for treating the high-salt wastewater by the halomonas comprises the following steps of:
a. gradually expanding and culturing the halomonas seed solution;
b. and adding the bacterial liquid with the expanded culture polyploidy volume into the high-salt wastewater for culture.
The step a is preferably that the halomonas of claim 1 is treatedCobetia sp.) And Meilinchi yeast with collection number of CGMCC No.18674 (Latin academic name)Metschnikowia bicuspidatePreservation date: 2019-10-12; preservation unit: chinese microorganismThe seed preservation management committee general microbiological center; deposit number: CGMCC No. 18674) according to the volume ratio of 1:2, preparing composite seed liquid for gradually expanding culture; and the step b is preferably to add 20-50 times of bacterial liquid by volume of expansion culture into the high-salt wastewater with the addition amount of 1-3% by volume percent, and shake culture for 60 hours at the temperature of 20-28 ℃ at the speed of 100-150 r/min.
The invention separates, purifies, identifies, optimizes and screens out the halomonas capable of utilizing the high-salt wastewater organic mattersCobetia sp.) The screened strain (CGMCC No. 20812) is used for treating the wastewater with high salt produced by kelp processing, so that the operation is simple, the cost is low, the COD removal rate of the wastewater with high salt produced by kelp processing is 84.82%, and the COD removal rate of the wastewater with high salt produced by undaria pinnatifida processing is 85.87%, thereby reaching the urban pipe network emission standard. In particular to the method for processing the undaria pinnatifida to produce high-salt wastewater by combining the halomonas and the quince yeast (CGMCC No. 18674), wherein the COD removal rate of the high-salt wastewater produced by the undaria pinnatifida processing is 86.24 percent.
Drawings
FIGS. 1 and 2 are graphs showing the COD removal rate and the biomass change with time of cultivation in example 1 of the present invention.
FIG. 3 is a graph showing the change of the COD removal rate and biomass with time of cultivation in example 2 of the present invention.
The preservation date of the halomonas strain: 2020-9-23;
preservation unit: china general microbiological culture Collection center (China Committee for culture Collection);
address: beijing, chaoyang area, north Chen Xi Lu 1;
deposit number: cgmccno.20812.
Date of collection of the mycelial bijiangensis strain: 2019-10-12;
preservation unit: china general microbiological culture Collection center (China Committee for culture Collection);
address: beijing, chaoyang area, north Chen Xi Lu 1;
deposit number: CGMCC No.18674.
Detailed Description
Example 1:
the invention adopts a strain which is a strain capable of utilizing high-salt wastewater organic matters and is selected by separation, purification, identification and optimization as a halomonas strainCobetia sp.) The strain is preserved in China general microbiological culture Collection center, with the code WNWAW-C and the preservation number of CGMCC No.20812.
The method for treating the high-salt wastewater by using the halomonas comprises the following steps of:
a. gradually expanding and culturing the halomonas (CGMCC No. 20812) seed solution;
b. the bacterial liquid which is 8 times of the volume of the expansion culture is respectively added into the wastewater which is processed by kelp and is produced by undaria pinnatifida and is produced by high salt in the adding amount of 5 percent by volume, and the bacterial liquid is cultivated by shaking at 150 r/min for 48 hours at 20 ℃.
The high-salt wastewater produced by processing kelp and the high-salt wastewater produced by processing undaria pinnatifida treated by the method are measured, and the results are respectively shown in figures 1 and 2. The result shows that the COD removal rate of the high-salt wastewater produced by kelp processing is 84.82 percent, and the COD removal rate of the high-salt wastewater produced by undaria pinnatifida processing is 85.87 percent.
Example 2:
the method for treating the high-salt wastewater by using the halomonas comprises the following steps of:
a. the Salmonella typhimurium (CGMCC No. 20812) and the two-point Meiqi microzyme with the preservation number of CGMCC No.18674 are addedMetschnikowia bicuspidate) The volume ratio is 1:2, preparing composite seed liquid for gradually expanding culture;
b. the bacterial liquid which is cultivated for 20 times of volume is added into the wastewater which is processed by undaria pinnatifida and produces high salt by adding the bacterial liquid with the volume percentage of 1 percent, and the bacterial liquid is cultivated for 60 hours by shaking at 150 r/min under the condition of 20 ℃.
COD measurement is carried out on the wastewater with high salt produced by processing the undaria pinnatifida treated by the method by using a potassium permanganate oxidation method, and the result is shown in figure 3. The result shows that the COD removal rate of the wastewater produced by processing the undaria pinnatifida is 86.24%.
Claims (2)
1. Salmonella spCobetia sp) Has been deposited inChina general microbiological culture Collection center (CGMCC) with a collection number of CGMCC No.20812.
2. A method for treating kelp or undaria pinnatifida processing high-salt wastewater by using halomonas according to claim 1, which is characterized by comprising the following steps:
a. gradually expanding the halomonas seed solution according to claim 1;
b. adding the bacterial liquid with the expanded culture polyploidy volume into the wastewater produced by processing kelp or undaria pinnatifida to culture.
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| CN202011252442.7A CN112501057B (en) | 2020-11-11 | 2020-11-11 | Halomonas and method for treating high-salt wastewater |
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|---|---|---|---|
| CN202011252442.7A CN112501057B (en) | 2020-11-11 | 2020-11-11 | Halomonas and method for treating high-salt wastewater |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112501057A CN112501057A (en) | 2021-03-16 |
| CN112501057B true CN112501057B (en) | 2024-02-02 |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100817708B1 (en) * | 2007-12-31 | 2008-03-27 | 정래국 | A novel halophilic halomonas sp. having an excellent digestive effect of organic material, a soil conditioner for the reclaimed land using said strain and a method for plant growth-promoting thereof |
| CN102168031A (en) * | 2010-10-29 | 2011-08-31 | 中国水产科学研究院南海水产研究所 | Marine organic matter degradation Halomonas sp. strain GHMC414 and application thereof |
| CN105861377A (en) * | 2016-05-09 | 2016-08-17 | 中国水产科学研究院南海水产研究所 | Halomonas strain GHMC422 used for degradation of ocean organic matters and application of GHMC422 |
| WO2018037081A1 (en) * | 2016-08-25 | 2018-03-01 | Covestro Deutschland Ag | Formate catalysis from hypersaline environments by a halotolerant halomonas sp. |
| CN110760470A (en) * | 2019-12-05 | 2020-02-07 | 大连海洋大学 | Halomonas with aerobic denitrification function and application thereof |
| CN111705018A (en) * | 2020-06-28 | 2020-09-25 | 黄河三角洲京博化工研究院有限公司 | Halomonas flava capable of efficiently degrading organic matters and full salt in high-salinity wastewater and application of Halomonas flava |
| CN111893080A (en) * | 2020-09-05 | 2020-11-06 | 深圳市长隆科技有限公司 | Halomonas YFX-6 and application thereof |
| CN111909885A (en) * | 2020-09-23 | 2020-11-10 | 苏州道源华智环保科技有限公司 | Salt-tolerant COD-reducing strain, culture method and application |
-
2020
- 2020-11-11 CN CN202011252442.7A patent/CN112501057B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100817708B1 (en) * | 2007-12-31 | 2008-03-27 | 정래국 | A novel halophilic halomonas sp. having an excellent digestive effect of organic material, a soil conditioner for the reclaimed land using said strain and a method for plant growth-promoting thereof |
| CN102168031A (en) * | 2010-10-29 | 2011-08-31 | 中国水产科学研究院南海水产研究所 | Marine organic matter degradation Halomonas sp. strain GHMC414 and application thereof |
| CN105861377A (en) * | 2016-05-09 | 2016-08-17 | 中国水产科学研究院南海水产研究所 | Halomonas strain GHMC422 used for degradation of ocean organic matters and application of GHMC422 |
| WO2018037081A1 (en) * | 2016-08-25 | 2018-03-01 | Covestro Deutschland Ag | Formate catalysis from hypersaline environments by a halotolerant halomonas sp. |
| CN110760470A (en) * | 2019-12-05 | 2020-02-07 | 大连海洋大学 | Halomonas with aerobic denitrification function and application thereof |
| CN111705018A (en) * | 2020-06-28 | 2020-09-25 | 黄河三角洲京博化工研究院有限公司 | Halomonas flava capable of efficiently degrading organic matters and full salt in high-salinity wastewater and application of Halomonas flava |
| CN111893080A (en) * | 2020-09-05 | 2020-11-06 | 深圳市长隆科技有限公司 | Halomonas YFX-6 and application thereof |
| CN111909885A (en) * | 2020-09-23 | 2020-11-10 | 苏州道源华智环保科技有限公司 | Salt-tolerant COD-reducing strain, culture method and application |
Non-Patent Citations (1)
| Title |
|---|
| 杨婷婷.甘露醇利用菌的筛选及在褐藻加工废水中的应用.《中国优秀硕士学位论文全文数据库 基础科学辑》.2020,(第03期),第2.3节，2.4节，第3章和第4章. * |
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| CN112501057A (en) | 2021-03-16 |
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