CN112501038B - Pichia guilliermondii and method for treating high-salt wastewater - Google Patents
Pichia guilliermondii and method for treating high-salt wastewater Download PDFInfo
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- CN112501038B CN112501038B CN202011250991.0A CN202011250991A CN112501038B CN 112501038 B CN112501038 B CN 112501038B CN 202011250991 A CN202011250991 A CN 202011250991A CN 112501038 B CN112501038 B CN 112501038B
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- salt wastewater
- guilliermondii
- processing
- pichia
- culture
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- 239000002351 wastewater Substances 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241000235048 Meyerozyma guilliermondii Species 0.000 title claims abstract description 13
- 241000512259 Ascophyllum nodosum Species 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 238000004321 preservation Methods 0.000 claims abstract description 12
- 241001261506 Undaria pinnatifida Species 0.000 claims abstract description 10
- 230000001580 bacterial effect Effects 0.000 claims abstract description 8
- 238000009629 microbiological culture Methods 0.000 claims abstract description 8
- 208000020584 Polyploidy Diseases 0.000 claims abstract description 3
- 241000235648 Pichia Species 0.000 claims description 2
- 241000235058 Komagataella pastoris Species 0.000 abstract description 5
- 238000012258 culturing Methods 0.000 abstract description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 206010033546 Pallor Diseases 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241000311506 Meyerozyma Species 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 238000009938 salting Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000003902 seawater pollution Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
Abstract
The invention discloses a pichia pastoris strainMeyerozyma guilliermondii) Has been preserved in China general microbiological culture Collection center with a preservation number of CGMCC No.18675. The method for treating the high-salt wastewater by using the pichia guilliermondii comprises the following steps of: gradually expanding and culturing the seed liquid of the bicuspension mermaid microzyme; and adding the bacterial liquid with the expanded culture polyploidy volume into the high-salt wastewater for culture. The method is simple to operate and low in cost, the COD removal rate of the high-salt wastewater produced by kelp processing is 84.61%, the COD removal rate of the high-salt wastewater produced by undaria pinnatifida processing is 75.50%, and the method meets the urban pipe network emission standard.
Description
Technical Field
The invention relates to a bacterium for wastewater treatment and a treatment method, in particular to a pichia pastoris (Latin academic name)Meyerozyma guilliermondiiPreservation date: 2019-10-12; preservation unit: china general microbiological culture Collection center (China Committee for culture Collection); deposit number: CGMCC No. 18675) and a method for treating high-salt wastewater.
Background
In order to prevent fresh kelp and undaria pinnatifida from spoiling and deteriorating, the collected fresh kelp is usually subjected to blanching, cooling and salting treatment immediately so as to realize sterilization and dehydration. The blanching water is seawater which is repeatedly heated to about 95 ℃, and is recycled for 8-10 hours to obtain high-salt wastewater, wherein the content of organic matters (phycocolloids, mannitol and the like) and inorganic matters is high, the COD value can reach 1200-2100, and the high-salt wastewater can cause seawater pollution if directly discharged, so that the high-salt wastewater can be discharged to a city pipe network after being treated to reach standards according to regulations. At present, related reports on the treatment of industrial wastewater by using biotechnology are presented, and the method has the characteristics of simple operation, low price and the like, but is limited to the treatment of fresh water wastewater. Although the MVR high-efficiency evaporation crystallization system can be used for treating high-salt wastewater produced by kelp and undaria pinnatifida processing, the cost is high (100-120 yuan/ton), and the production benefit of a processing plant is directly affected.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provides pichia pastoris and a method for treating high-salt wastewater.
The technical scheme of the invention is as follows: pichia pastorisMeyerozyma guilliermondii) Has been preserved in China general microbiological culture Collection center with a preservation number of CGMCC No.18675.
The method for treating the high-salt wastewater by the pichia guilliermondii comprises the following steps of:
a. gradually expanding and culturing the pichia guilliermondii seed liquid;
b. and adding the bacterial liquid with the expanded culture polyploidy volume into the high-salt wastewater for culture.
The step a is preferably to combine the Pichia guilliermondii with the two-point Meiqi yeast (Latin academic name) with the collection number of CGMCC No.18674Metschnikowia bicuspidatePreservation date: 2019-10-12; preservation unit: china general microbiological culture Collection center (China Committee for culture Collection); deposit number: CGMCC No. 18674) according to the volume ratio of 1: and 1, preparing composite seed liquid for gradual expansion culture, wherein in the step b, bacterial liquid with the volume which is 8-10 times of that of the expansion culture is added into high-salt wastewater in an addition amount of 1-3% by volume, and shake culture is carried out for 60 hours at the temperature of 10-28 ℃ at the speed of 100-150 r/min.
The invention separates, purifies, identifies, optimizes and screens out the Pichia pastoris which can utilize the organic matters in the high-salt wastewaterMeyerozyma guilliermondiiCGMCC No. 18675), the selected strain is used for treating the high-salt wastewater produced by kelp processing or undaria pinnatifida processing, the operation is simple, the cost is low, the COD removal rate of the high-salt wastewater produced by kelp processing is 82.92%, the COD removal rate of the high-salt wastewater produced by undaria pinnatifida processing is 85.60%, and the urban pipe network emission standard is reached. In particular to the method for processing kelp to produce high-salt wastewater by combining pichia pastoris (CGMCC No. 18675) and Meilinchus bikinus (CGMCC No. 18674), wherein the COD removal rate of the high-salt wastewater produced by processing kelp reaches 85.98 percent.
Drawings
FIGS. 1 and 2 are graphs showing the COD removal rate and the biomass change with time of cultivation in example 1 of the present invention.
FIG. 3 is a graph showing the change of the COD removal rate and biomass with time of cultivation in example 2 of the present invention.
Pichia guilliermondii strain preservation date: 2019-10-12;
preservation unit: china general microbiological culture Collection center (China Committee for culture Collection);
address: beijing, chaoyang area, north Chen Xi Lu 1;
deposit number: CGMCC No.18675.
Date of collection of the mycelial bijiangensis strain: 2019-10-12;
preservation unit: china general microbiological culture Collection center (China Committee for culture Collection);
address: beijing, chaoyang area, north Chen Xi Lu 1;
deposit number: CGMCC No.18674.
Detailed Description
Example 1:
the invention separates, purifies, identifies, optimizes and screens out the strain which can utilize the high-salt waste water organic matters as the Pichia guilliermondii strainMeyerozyma guilliermondii) The strain is preserved in China general microbiological culture Collection center, with the code HSKeW-M and the preservation number of CGMCC No.18675.
The method for treating the high-salt wastewater by using the pichia guilliermondii comprises the following steps:
a. gradually expanding and culturing the seed liquid of the pichia guilliermondii (CGMCC No. 18675);
b. the bacterial liquid which is 8 times of the volume of the expansion culture is respectively added into the wastewater which is processed by kelp and is produced by undaria pinnatifida and is produced by high salt in the adding amount of 5 percent by volume, and the bacterial liquid is cultivated by shaking at 150 r/min for 60 hours at 20 ℃.
The high-salt wastewater produced by processing kelp and the high-salt wastewater produced by processing undaria pinnatifida treated by the method are measured, and the results are respectively shown in figures 1 and 2. The result shows that the COD removal rate of the high-salt wastewater produced by kelp processing is 84.61 percent, and the COD removal rate of the high-salt wastewater produced by undaria pinnatifida processing is 75.50 percent.
Example 2:
the method for treating the high-salt wastewater by using the bipointed merzizyweed comprises the following steps:
a. mixing the above Pichia guilliermondii (CGMCC No. 18675) with the two-point Mei-qi yeast with the preservation number of CGMCC No.18674Metschnikowia bicuspidate) The volume ratio is 1:1, preparing composite seed liquid and gradually expanding culture;
b. the bacterial liquid which is expanded and cultivated by 8 times of volume is added into the wastewater which is produced by processing kelp and is high in salt content by 3 percent of volume percent, and the bacterial liquid is cultivated by a shaking table at 150 r/min for 60 hours at 20 ℃.
COD measurement is carried out on the kelp processing high-salt wastewater treated by the method by using a potassium permanganate oxidation method, and the result is shown in figure 3. The result shows that the COD removal rate of the wastewater with high salt content produced by kelp processing is 85.98%.
Claims (2)
1. Pichia pastorisMeyerozyma guilliermondii) Has been preserved in China general microbiological culture Collection center with a preservation number of CGMCC No.18675.
2. A method for treating kelp or undaria pinnatifida processing high-salt wastewater by using the pichia guilliermondii according to claim 1, which is characterized by comprising the following steps:
a. gradually expanding the pichia guilliermondii seed solution according to claim 1;
b. adding the bacterial liquid with the expanded culture polyploidy volume into the wastewater produced by processing kelp or undaria pinnatifida to culture.
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| CN202011250991.0A CN112501038B (en) | 2020-11-11 | 2020-11-11 | Pichia guilliermondii and method for treating high-salt wastewater |
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|---|---|---|---|
| CN202011250991.0A CN112501038B (en) | 2020-11-11 | 2020-11-11 | Pichia guilliermondii and method for treating high-salt wastewater |
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| Publication Number | Publication Date |
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| CN112501038A CN112501038A (en) | 2021-03-16 |
| CN112501038B true CN112501038B (en) | 2024-02-02 |
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| CN202011250991.0A Active CN112501038B (en) | 2020-11-11 | 2020-11-11 | Pichia guilliermondii and method for treating high-salt wastewater |
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| CN114456958B (en) * | 2021-12-20 | 2023-08-22 | 辽宁师范大学 | Salt-tolerant pichia pastoris strain with azo dye degradation function, culture method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2023118A (en) * | 1978-04-26 | 1979-12-28 | Ajinomoto Kk | Method of waste water treatment by yeast |
| KR20000056878A (en) * | 1999-02-27 | 2000-09-15 | 김준엽 | Production of Pichia guilliermondii Biomass Using Saline Medium and Process for Treating Waste by Employing Same |
| CN102766579A (en) * | 2011-05-05 | 2012-11-07 | 中国科学院生态环境研究中心 | Yeast strain for generating surfactant and application thereof |
| CN106085889A (en) * | 2016-06-24 | 2016-11-09 | 四川大学 | Pichia guilliermondii 3 J15 and application thereof |
| CN106190875A (en) * | 2016-07-13 | 2016-12-07 | 江西沃邦兴环保科技有限公司 | Facultative Halophiles bacterial strain under a kind of high-salt tolerance environment and screening technique thereof and application |
| CN107354102A (en) * | 2017-02-13 | 2017-11-17 | 浙江大学 | The sugared Pichia guilliermondii bacterial strain of the resistance to height of one kind and its application |
-
2020
- 2020-11-11 CN CN202011250991.0A patent/CN112501038B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2023118A (en) * | 1978-04-26 | 1979-12-28 | Ajinomoto Kk | Method of waste water treatment by yeast |
| KR20000056878A (en) * | 1999-02-27 | 2000-09-15 | 김준엽 | Production of Pichia guilliermondii Biomass Using Saline Medium and Process for Treating Waste by Employing Same |
| CN102766579A (en) * | 2011-05-05 | 2012-11-07 | 中国科学院生态环境研究中心 | Yeast strain for generating surfactant and application thereof |
| CN106085889A (en) * | 2016-06-24 | 2016-11-09 | 四川大学 | Pichia guilliermondii 3 J15 and application thereof |
| CN106190875A (en) * | 2016-07-13 | 2016-12-07 | 江西沃邦兴环保科技有限公司 | Facultative Halophiles bacterial strain under a kind of high-salt tolerance environment and screening technique thereof and application |
| CN107354102A (en) * | 2017-02-13 | 2017-11-17 | 浙江大学 | The sugared Pichia guilliermondii bacterial strain of the resistance to height of one kind and its application |
Non-Patent Citations (1)
| Title |
|---|
| 杨婷婷.甘露醇利用菌的筛选及在褐藻加工废水中的应用.《中国优秀硕士学位论文全文数据库 基础科学辑》.2020,(第03期),第2.3节，2.4节，第3章和第4章. * |
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| CN112501038A (en) | 2021-03-16 |
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