CN103087920A - Mixotrophic scenedesmus and application thereof in sewage resource treatment - Google Patents
Mixotrophic scenedesmus and application thereof in sewage resource treatment Download PDFInfo
- Publication number
- CN103087920A CN103087920A CN2013100279759A CN201310027975A CN103087920A CN 103087920 A CN103087920 A CN 103087920A CN 2013100279759 A CN2013100279759 A CN 2013100279759A CN 201310027975 A CN201310027975 A CN 201310027975A CN 103087920 A CN103087920 A CN 103087920A
- Authority
- CN
- China
- Prior art keywords
- sewage
- scenedesmus
- grid algae
- zty1
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention relates to mixotrophic scenedesmus and an application in sewage resource treatment. The scenedesmus has strong anti-pollution capability and can grow stably in actual sewage (non-sterile), the heterotrophic capability is strong, the productivity of biomass and grease is high, namely organic matters in the sewage can be transformed to grease, nutrient substances in the sewage can be further utilized for absorbing carbon dioxide in atmosphere, nitrogen and phosphorus in the sewage can be removed in a sustained manner under the situation that an organic carbon source is used up, and when the sewage is taken as a matrix for inoculating the scenedesmus, the water quality can be purified, and the grease can be simultaneously accumulated, so that sewage resources can be effectively utilized, the waste can be changed to valuable things, and the mixotrophic scenedesmus further has the advantages of low operation cost, good quality of outlet water, simple and convenient technology, easiness in popularization and the like.
Description
Technical field
The present invention relates to water pollution control and production of biodiesel field, be specifically related to a strain mixotrophism type grid algae and the application in sewage recycling is processed thereof.
Background technology
In the water pollution control field, the conventional treatment process take activated sludge process as representative is difficult to realize the recycling to sewage, and the organism in sewage and inorganic salt run off with the processing of the sewage form with gas or mud, the present situation of having aggravated resource scarcity.In the production of biodiesel field, little algae is cultivated the key constraints that high cost is the little algae biodiesel commercialization of restriction, cultivate little algae take sewage as matrix cheap biomass material can be provided as the production of biofuel and allogenic material product thereof, thereby significantly reduce production costs.A little less than common high produce oil algae kind contamination resistance, be difficult to stable existence in the actual sewage that sterilized is processed, seriously limited the commercial applications based on little algae culture technique of sewage.Screening adaptation Sewage Environment, the suitable algae kind that oil offtake is high, pollutant removal is good, significant for commercially producing of little algal biomass products such as the recycling of realizing sewage and biofuel.
Summary of the invention
In order to overcome the shortcoming of above-mentioned prior art, the purpose of this invention is to provide a strain mixotrophism type grid algae and the application in sewage recycling is processed thereof, this little algae has advantages of the Sewage Environment of adaptation, oil offtake is high, pollutant removal good.
In order to achieve the above object, the technical scheme taked of the present invention is:
One strain mixotrophism type grid algae, grid algae (Scenedesmus sp.ZTY1) separates from Beijing sanitary sewage disposal factory, be preserved in China Microbial Culture Preservation Commission's common micro-organisms center (CGMCC) on January 14th, 2013, register on the books and be numbered 7059, the CGMCC address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode is 100101.
Described grid algae (Scenedesmus sp.ZTY1) coenobium is flat, is comprised of 2 or 4 cells, and colony's cell is listed on straight line, the cell ovalize, cell walls is level and smooth, wide 15～25 μ m of the colony of 4 cells, individual cells is wide is 3～6 μ m, long 9～12 μ m.
The application of one strain mixotrophism type grid algae in sewage recycling is processed first is inoculated into this grid algae in sewage, by nutritive elements such as the organism in the assimilation removal sewage of frustule and nitrogen phosphorus, it is characterized in that, concrete steps are:
1) inoculate grid algaes (Scenedesmus sp.ZTY1) in sewage, Initial seeding density is 1 * 10
5～1 * 10
6Individual/mL;
2) sewage that will inoculate after frustule is placed under nature or artificial lighting condition, the artificial lighting condition is: Light To Dark Ratio 14:10, light intensity 800～1300lux, keep the stir speed (S.S.) of 80-150r/min and the temperature of 5～25 ℃, make grid algae (Scenedesmus sp.ZTY1) utilize the nutrient in sewage to grow;
3) cultivate centrifugal results frustule after 2～45 days, discharge simultaneously and process rear water;
4) gathering in the crops the frustule that obtains can for the production of biofuel or other little algal biomass product after further processing, also can be inoculated in new sewage and continue on for sewage treatment process.
Described sewage is the preliminary sedimentation tank water outlet of general municipal sewage plant.
Beneficial effect of the present invention is: this strain grid algae can grow in the actual sewage environment, need not sewage is done sterilising treatment or additionally added nutrition, has the ability that the organism in sewage is converted into grease, and the nitrogen phosphorus in water body can be removed to very low level.At first utilize the nutritive element in grid algae assimilation absorption sewage, by certain way, the grid algae is separated from sewage again, obtain the very low sewage disposal water outlet of pollutant load, thereby reduce the risk of sewage disposal plant effluent regeneration, and obtain little algal biomass of high fat content.The present invention can be applicable to the production of little algal biomass products such as sewage disposal and biofuel.
Description of drawings
Fig. 1 is growth curve and the fat content variation diagram of grid algae under the intermittent type culture condition (Scenedesmus sp.ZTY1) in sanitary sewage.
Fig. 2 is growth curve and the Pollutant levels variation diagram of grid algae under the sequence batch (culture condition (Scenedesmus sp.ZTY1) in sanitary sewage.
Embodiment
The invention will be further described below in conjunction with embodiment and accompanying drawing:
Embodiment 1
Cultivate for a long time in the mode of intermittently cultivating the grease output that grid algae (Scenedesmus sp.ZTY1) can effectively be improved unit sewage.Adopt real life sewage to carry out intermittent type to grid algae (Scenedesmus sp.ZTY1) and cultivate, dissolved organic carbon in sewage (DOC) concentration is 112mgL
-1, total nitrogen (TN) concentration is 41.0mgL
-1, total phosphorus (TP) concentration is 8.4mgL
-1, Initial seeding density is about 7.5 * 10
5Individual mL
-1, under the condition of Light To Dark Ratio 14h:10h, 25 ℃ of temperature, intensity of illumination 800～1300lux, to cultivate after 21 days, grid algae (Scenedesmus sp.ZTY1) enters stationary phase, obtains the very low (TN:2.9mgL of carbon concentration of nitrogen and phosphorus after centrifugal results grid algae
-1, TP:0.07mgL
-1) three grades of water outlets of municipal effluent, this strain grid algae is respectively 92.9% and 99.1% to nitrogen and phosphorus removal rate in the water outlet of municipal effluent one-level.
During take sanitary sewage as substratum, this strain grid algae (Scenedesmus sp.ZTY1) is at grease yield and the TAG(triglyceride in stationary phase early stage (cultivating 21 days), the starting material of preparing biological diesel oil) output is respectively 102mgL
-1And 32mgL
-1, grease yield and the TAG(triglyceride in later stage stationary phase (cultivating 45 days), the starting material of preparing biological diesel oil) and output is respectively 163mgL
-1And 148mgL
-1Grid algae under the intermittent type culture condition (Scenedesmus sp.ZTY1) in sanitary sewage growth curve and the fat content variation diagram as shown in Figure 1.Can find out from growth curve chart, grid algae ZTY1 grows fine in real life sewage, and the adaptive phase is short, enters stationary phase after about 10 days, and sewage is had good adaptability.Can find out from grease and TAG output figure, the proper extension incubation time can significantly increase the grease yield of unit sewage, promotes the frustule oil and fat accumulation.
The mode high-density culture grid algae ZTY1 that cultivates with sequence batch (can effectively reduce the sewage disposal time, improves processing efficiency.(DOC is that 72.9, TN is 50.4mgL to adopt real life sewage
-1, TP is 3.5mgL
-1) grid algae (Scenedesmus sp.ZTY1) is carried out the sequence batch (cultivation, culture condition is: intensity of illumination 800～1300lux, Light To Dark Ratio 14h:10h, 25 ℃ of temperature, stir speed (S.S.) 150r/min.The Initial seeding density of grid algae (Scenedesmus sp.ZTY1) is 1.28 * 10
6Individual mL
-1, after each treatment cycle finishes, centrifugal draining, the frustule of results 50% is used for preparing biological diesel oil, and the frustule of residue 50% is stayed in system, adds new sanitary sewage, continues the cultivation operation of next cycle.The algae variable density of grid algae (Scenedesmus sp.ZTY1) and to the removal of pollutants effect as shown in Figure 2 in culturing process intermittently, it is three days that hydraulic detention time is set, in sewage, the clearance of DOC, TN and TP can reach respectively 75%, 97% and 98%, algae cell density raises rapidly with the reduction of pollutant load in sewage, and biomass yield and grease productive rate reach as high as 181mgL
-1D
-1And 37.9mgL
-1D
-1(with dry weight basis).
Claims (4)
1. a strain mixotrophism type grid algae, it is characterized in that: grid algae (Scenedesmus sp.ZTY1) separates from Beijing sanitary sewage disposal factory, be preserved in China Microbial Culture Preservation Commission's common micro-organisms center (CGMCC) on January 14th, 2013, register on the books and be numbered 7059, the CGMCC address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode is 100101.
2. a strain mixotrophism type grid algae according to claim 1, it is characterized in that: described grid algae (Scenedesmus sp.ZTY1) coenobium is flat, formed by 2 or 4 cells, colony's cell is listed on straight line, the cell ovalize, cell walls is level and smooth, wide 15～25 μ m of the colony of 4 cells, individual cells is wide is 3～6 μ m, long 9～12 μ m.
3. according to claim 1 with 2 described strain mixotrophism type grid algaes, it is characterized in that: the application of a strain mixotrophism type grid algae in sewage recycling is processed, first this grid algae is inoculated in sewage, by nutritive elements such as the organism in the assimilation removal sewage of frustule and nitrogen phosphorus, it is characterized in that, concrete steps are:
1) inoculate grid algaes (Scenedesmus sp.ZTY1) in sewage, Initial seeding density is 1 * 10
5～1 * 10
6Individual/mL;
2) sewage that will inoculate after frustule is placed under nature or artificial lighting condition, the artificial lighting condition is: Light To Dark Ratio 14:10, light intensity 800～1300lux, keep the stir speed (S.S.) of 80-150r/min and the temperature of 5～25 ℃, make grid algae (Scenedesmus sp.ZTY1) utilize the nutrient in sewage to grow;
3) cultivate centrifugal results frustule after 2～45 days, discharge simultaneously and process rear water;
4) gathering in the crops the frustule that obtains can for the production of biofuel or other little algal biomass product after further processing, also can be inoculated in new sewage and continue on for sewage treatment process.
4. a strain mixotrophism type grid algae according to claim 3, it is characterized in that: described sewage is the preliminary sedimentation tank water outlet of general municipal sewage plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310027975.9A CN103087920B (en) | 2013-01-24 | 2013-01-24 | Mixotrophic scenedesmus and application thereof in sewage resource treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310027975.9A CN103087920B (en) | 2013-01-24 | 2013-01-24 | Mixotrophic scenedesmus and application thereof in sewage resource treatment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103087920A true CN103087920A (en) | 2013-05-08 |
| CN103087920B CN103087920B (en) | 2014-10-08 |
Family
ID=48201061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310027975.9A Active CN103087920B (en) | 2013-01-24 | 2013-01-24 | Mixotrophic scenedesmus and application thereof in sewage resource treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103087920B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484374A (en) * | 2013-10-14 | 2014-01-01 | 哈尔滨工业大学 | Scenedesmus obliquus capable of synchronously processing municipal sewage and accumulating grease |
| CN104513799A (en) * | 2013-09-29 | 2015-04-15 | 重庆工商大学 | New idea of utilizing sewage to culture oil-rich algae and also purify water |
| CN104630295A (en) * | 2015-03-08 | 2015-05-20 | 中国海洋大学 | Method for treating municipal secondary wastewater and producing grease by using immobilized microalgae |
| CN108641965A (en) * | 2018-04-16 | 2018-10-12 | 天津大学 | A kind of optimization method of and foster pattern scenedesmus culture |
| CN113214996A (en) * | 2021-05-18 | 2021-08-06 | 武汉理工大学 | Scenedesmus as well as culture method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565674A (en) * | 2009-05-13 | 2009-10-28 | 清华大学 | Low-nutrition scenedesmus and application thereof in sewage deep treatment |
-
2013
- 2013-01-24 CN CN201310027975.9A patent/CN103087920B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565674A (en) * | 2009-05-13 | 2009-10-28 | 清华大学 | Low-nutrition scenedesmus and application thereof in sewage deep treatment |
Non-Patent Citations (2)
| Title |
|---|
| 安晓雯等: "固定化栅藻对市政污水中氮、磷营养盐的深度净化", 《中南民族大学学报（自然科学版）》 * |
| 张恩栋等: "固定化的栅藻深度脱氮和除磷能力", 《植物生理学通讯》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104513799A (en) * | 2013-09-29 | 2015-04-15 | 重庆工商大学 | New idea of utilizing sewage to culture oil-rich algae and also purify water |
| CN103484374A (en) * | 2013-10-14 | 2014-01-01 | 哈尔滨工业大学 | Scenedesmus obliquus capable of synchronously processing municipal sewage and accumulating grease |
| CN103484374B (en) * | 2013-10-14 | 2015-01-14 | 哈尔滨工业大学 | Scenedesmus obliquus capable of synchronously processing municipal sewage and accumulating grease |
| CN104630295A (en) * | 2015-03-08 | 2015-05-20 | 中国海洋大学 | Method for treating municipal secondary wastewater and producing grease by using immobilized microalgae |
| CN108641965A (en) * | 2018-04-16 | 2018-10-12 | 天津大学 | A kind of optimization method of and foster pattern scenedesmus culture |
| CN113214996A (en) * | 2021-05-18 | 2021-08-06 | 武汉理工大学 | Scenedesmus as well as culture method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103087920B (en) | 2014-10-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101285075B (en) | Coupling process of marsh gas fermentation and culture of autotrophic freshwater microalgae | |
| CN102442726B (en) | Fungi-mediated microalgae immobilization wastewater treatment method | |
| CN105174476B (en) | Activated sludge and microalgae coupling particle system and its structure and operation method for wastewater treatment | |
| CN101921811B (en) | Method for culturing microalgae | |
| Wang et al. | Growth enhancement of biodiesel-promising microalga Chlorella pyrenoidosa in municipal wastewater by polyphosphate-accumulating organisms | |
| CN102746992B (en) | Method for culturing chlorella by heterotrophism with sludge hydrolysate | |
| CN102337302A (en) | Method for biologically purifying marsh gas and recycling waste of marsh gas | |
| CN103087920B (en) | Mixotrophic scenedesmus and application thereof in sewage resource treatment | |
| Ramaraj et al. | Potential evaluation of biogas production and upgrading through algae | |
| WO2006017991A1 (en) | Stepped sequential treatment method for municipal domestic refuse | |
| CN103074231B (en) | Method for producing microalgae by utilizing industrial waste water and waste gas of biobutanol, and application of method | |
| CN104830942A (en) | Biological alcohol production technique of organic sewage | |
| CN102815839A (en) | Method and device for low-carbon emission energy regeneration by coupling waste gas and waste water | |
| CN105776745A (en) | Biological treatment method of high-ammonia nitrogen pig raising biogas slurry | |
| Solmaz et al. | Effect of sludge retention time on biomass production and nutrient removal at an algal membrane photobioreactor | |
| CN101586074B (en) | Algae cultivation system with sewage | |
| CN102746991B (en) | Method for carrying out heterotrophic culture on chlorella with acid-producing wastewater | |
| CN103555778A (en) | Method for generating energy from hydrogen production bacteria and oily microalgae in stair-coupling mode | |
| CN102363794B (en) | Method for producing hydrogen through kitchen waste enzymolysis and reinforced dark fermentation | |
| CN107177506B (en) | Method for optimizing sludge dewatering liquid culture chlorella by using crude glycerol | |
| CN110484570A (en) | A method of utilizing kitchen garbage co-producing hydrogen and insect protein | |
| CN105400697A (en) | Method for purifying undiluted anaerobic fermentation tail liquid by growing microalgae in carbon dioxide environment | |
| CN108611276A (en) | A method of recycling treatment being carried out to distillery waste water using microalgae | |
| Sengupta et al. | A critical review on phycoremediation of pollutants from wastewater—a novel algae-based secondary treatment with the opportunities of production of value-added products | |
| CN110194567A (en) | A kind of processing method of sludge hydrolysis, acidification liquid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |