CN104059870A - Desalination method for cooperatively treating edible fungus salinizing water based on halophilic microorganism complex inoculant and three-stage type membrane technology - Google Patents
Desalination method for cooperatively treating edible fungus salinizing water based on halophilic microorganism complex inoculant and three-stage type membrane technology Download PDFInfo
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Abstract
The invention discloses a desalination method for cooperatively treating edible fungus salinizing water based on halophilic microorganism complex inoculants and a three-stage type membrane technology. The method comprises the following steps: adding a microorganism complex inoculant I into the edible fungus salinizing water, and aerating and culturing for 5 days; then, carrying out three-stage type membrane treatment on the salinizing water to realize desalting; and finally, adding a microorganism complex inoculant II into the salinizing water subjected to three-stage type membrane treatment, and aerating and culturing for 4 days. The method disclosed by the invention has the advantages that the cost is reduced, the treatment method is more ecological and safer; a desalination rate of the finally obtained edible fungus salinizing water can be over 97%.
Description
Technical field
The present invention relates to the processing technology field of high-salt wastewater, in particular a kind of based on halophilic microorganism composite fungus agent and syllogic membrane technique associated treatment edible mushrooms salt marsh water desalting method.
Background technology
The salt marsh of edible mushrooms is a kind of Edible mushroom processing adopting at present extensively, the mode of preservation, Edible mushroom processing can be become to convenient and store and edible tinned pre-or bagged instant food etc.In the salting process of edible mushrooms, salt addition is up to 20%-35%, therefore, in the salting process of edible mushrooms and desalination operation afterwards, produced a large amount of high-salt wastewaters, its salt concn about 22%, because these waste water saltiness are very high, its biodegradability is poor, is difficult to process, and directly discharge produces again serious problem of environmental pollution.Traditional desalting method all has some limitations as evaporation, film processing, plastic resin treatment etc. in desalination condition.As the effect of evaporative desalination is subject to the restriction of other impurity in water (such as organic content etc.) larger, waste water resource on the other hand; Film is processed higher to the content requirement of salt, conventionally for monovalent salt (Na
+) can only make salt concn be reduced to 7% left and right, if improve again ratio of desalinization, and from energy consumption, facility investment, all can there be problem several aspects such as film tolerance; And resin desalination is very high to the requirement of water inlet salts contg, need to, compared with low saline salinity, can only use as the advanced desalination of salt accumulated water, and cost compare be high.These desalination modes all can not well realize the efficient desalination of salt accumulated water, cause in addition in addition the possibility of secondary pollution.General municipal wastewater is processed the activated sludge process of using, and because the salt concn in edible mushrooms salt accumulated water is too high, microorganism is most of suppressed, also can not get good processing.Therefore, there is the not high series of problems of purity salt that desalting efficiency is low, desalination cost is high, obtain in enterprise's salt marsh water desalination at present.
The salt accumulated water of edible mushrooms, except containing very high salt concn, also contains abundant nutritive ingredient.If can make edible mushrooms salt accumulated water become the edible mushrooms salt accumulated water that contains abundant nutrition composition of low salt concn, can be for developing other nutritive food, such as, the low salt marsh water nutrition of exploitation edible mushrooms face, the low salt marsh water nutrition biscuit of edible mushrooms or the low salt accumulated water of edible mushrooms organic feed etc.This has not only solved problem of environmental pollution, has also reduced cost waste, thereby has turned waste into wealth.
Summary of the invention
Technical problem to be solved by this invention is the problem existing in processing edible mushrooms salt marsh water desalination method for prior art, provides a kind of based on halophilic microorganism composite fungus agent and syllogic membrane technique associated treatment edible mushrooms salt marsh water desalting method.
Technical scheme of the present invention is as follows:
Based on halophilic microorganism composite fungus agent and a syllogic membrane technique associated treatment edible mushrooms salt marsh water desalting method, its step is as follows:
(1) activation of bacterial classification
By bacterial classification Nesterenkonia halophile, Halomonas Xinjiangensis, Halogranum rubrum, Actinopolyspora halophila and Halopelagius inordinatus activation culture on activation medium respectively, soak time is 5d-7d; Wherein, described strain activation and culture based component is: casein hydrolyzate 7.5g, fish peptone 0.5g, yeast extract paste 3g, Trisodium Citrate 0.1g, sodium-chlor 150g, potassium primary phosphate 0.3g, magnesium sulfate 0.05g, Manganous chloride tetrahydrate 0.0001g, distilled water 1000mL, pH7.2;
T bacteria etragenococcus halophilus, Salimicrobium halophilum, Saccharomyces cerevisiae, Bacillus subtilis and Weissella hellenica are activated respectively to 4d on activation medium; Wherein, described activation culture based component is: peptone 5g, yeast extract paste 3g, Trisodium Citrate 0.1g, casein hydrolysate 0.5g, sodium-chlor 50g, potassium primary phosphate 0.3g, magnesium sulfate 0.05g, distilled water 1000mL, pH7.2;
(2) cultivate altogether
By mass ratio mixing such as bacterial classification Nesterenkonia halophile, Halomonas Xinjiangensis, Halogranum rubrum, Actinopolyspora halophila and Halopelagius inordinatus after activation press, then add shaking table in the liquid nutrient medium that 100ml cultivates altogether to cultivate 4-5d, make complex microbial inoculum I; The composition of the described liquid nutrient medium of cultivating is altogether: peptone 10g, extractum carnis 3g, glucose 0.1g, yeast extract paste 0.2g, potassium primary phosphate 0.15g, Sodium.alpha.-ketopropionate 0.3g, magnesium sulfate 0.05g, iron protochloride 0.01g, calcium chloride 0.01g, sodium-chlor 150g, distilled water 1000ml, pH7.2; Adopt the described liquid nutrient medium of cultivating altogether and method to cultivate t bacteria etragenococcus halophilus, Salimicrobium halophilum, Saccharomyces cerevisiae, Bacillus subtilis and Weissella hellenica, wherein the consumption of sodium-chlor is adjusted into 50g, obtains complex microbial inoculum II;
(3) domestication of complex microbial inoculum
Complex microbial inoculum I after common cultivation and complex microbial inoculum II are put into respectively in edible mushrooms salt accumulated water, the salt concentration of initial salt accumulated water is respectively 10% and 1%, adopt the method that progressively improves salt marsh salinity water to tame, after a stage finishes, the complex microbial inoculum in upper stage is inoculated in the salt accumulated water of next high salt concentration, each salt accumulated water salt concn improves respectively 5% and 2%, so until 30% and 10% target salt concn;
(4) salt marsh water treatment
The desalination of the first step complex microbial inoculum I: the complex microbial inoculum I of access after high salt concentration domestication in edible mushrooms salt accumulated water, aeration is cultivated 5d;
Second step syllogic film is processed desalination: the salt accumulated water after the first step is processed, through the nylon filtering net pre-treatment of 5um, then adopts one-level multi-stage type reverse-osmosis treated;
The 3rd step microbial composite fungus agent II desalination: the complex microbial inoculum II of access after domestication in the salt accumulated water after syllogic film is processed, aeration is cultivated 4d.
The deposit number of described bacterial classification Nesterenkonia halophile is KCTC19048; The deposit number of Halomonas Xinjiangensis is CCTCC AB208329; The deposit number of Halogranum rubrum is JCM15772; The deposit number of Actinopolyspora halophila is JCM3278; The deposit number of Halopelagius inordinatus is JCM15773; The deposit number of Tetragenococcus halophilus is CICC10286; The deposit number of Salimicrobium halophilum is CICC10482; The deposit number of Saccharomyces cerevisiae is CICC31443; The deposit number of Bacillus subtilis is CICC21377; The deposit number of Weissella hellenica is CGMCC1.2513.
Described desalting method, in step (4), the inoculum size of complex microbial inoculum I is 5%wt; The inoculum size of complex microbial inoculum II is 5%wt.
The invention has the beneficial effects as follows: the three-wave-length desalting method in (1) the present invention can be realized the efficient desalination of salt accumulated water thoroughly, this provides condition for follow-up salt marsh Water reuse.(2) microorganism desalination can reduce the secondary pollution in demineralising process greatly, and microorganism desalination cost is lower simultaneously, and often, treatment process is ecological safety more for reusable edible.(3) desalting method of the present invention is simple to operate, in industrial production, easily realizes.And the edible mushrooms salt marsh water desalination rate finally obtaining can reach more than 97%.
Accompanying drawing explanation
Fig. 1 is one-level multistage reverse osmosis flow process.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment
1, bacterial classification used source
(1) Nesterenkonia halophile (deposit number KCTC19048) is purchased from Korea S's typical case's culture collection center.Halomonas Xinjiangensis (deposit number CCTCC AB208329) is purchased from Chinese Typical Representative culture collection center.Halogranum rubrum (deposit number JCM15772), Actinopolyspora halophila (deposit number JCM3278) and Halopelagius inordinatus (deposit number JCM15773) are all purchased from Japanese microbial preservation center.
(2) Tetragenococcus halophilus (deposit number CICC10286), Salimicrobium halophilum (deposit number CICC10482), Saccharomyces cerevisiae (deposit number CICC31443) and Bacillus subtilis (deposit number CICC21377) are all purchased from Chinese industrial microbial strains preservation administrative center.Weissella hellenica (deposit number CGMCC1.2513) is purchased from Chinese common micro-organisms culture presevation administrative center.
2, the activation of bacterial classification
(1) by bacterial classification Nesterenkonia halophile, Halomonas Xinjiangensis, Halogranum rubrum, Actinopolyspora halophila and Halopelagius inordinatus activation culture on activation medium respectively, soak time is 5d-7d.Strain activation and culture based component is: casein hydrolyzate 7.5g, fish peptone 0.5g, yeast extract paste 3g, Trisodium Citrate 0.1g, sodium-chlor 150g, potassium primary phosphate 0.3g, magnesium sulfate 0.05g, Manganous chloride tetrahydrate 0.0001g, distilled water 1000mL, pH7.2.
(2) t bacteria etragenococcus halophilus, Salimicrobium halophilum, Saccharomyces cerevisiae, Bacillus subtilis and Weissella hellenica are activated respectively to 4d on activation medium.Activation culture based component is: peptone 5g, yeast extract paste 3g, Trisodium Citrate 0.1g, casein hydrolysate 0.5g, sodium-chlor 50g, potassium primary phosphate 0.3g, magnesium sulfate 0.05g, distilled water 1000mL, pH7.2.
3, cultivate altogether
Bacterial classification Nesterenkonia halophile, Halomonas Xinjiangensis, Halogranum rubrum, Actinopolyspora halophila and Halopelagius inordinatus after activation are mixed by equal proportion (mass ratio), then add shaking table in the liquid nutrient medium that 100ml cultivates altogether to cultivate 4-5d, make complex microbial inoculum I.Cultivating altogether the medium component adopting is: peptone 10g, extractum carnis 3g, glucose 0.1g, yeast extract paste 0.2g, potassium primary phosphate 0.15g, Sodium.alpha.-ketopropionate 0.3g, magnesium sulfate 0.05g, iron protochloride 0.01g, calcium chloride 0.01g, sodium-chlor 150g, distilled water 1000ml, pH7.2.Similarly, adopt described substratum and method of cultivating altogether to cultivate t bacteria etragenococcus halophilus, Salimicrobium halophilum, Saccharomyces cerevisiae, Bacillus subtilis and Weissella hellenica, wherein the consumption of sodium-chlor is adjusted into 50g, obtains complex microbial inoculum II.
4, the domestication of complex microbial inoculum
Complex microbial inoculum I after common cultivation and complex microbial inoculum II are put into respectively to (salt concentration of initial salt accumulated water is respectively 10% and 1%) in edible mushrooms salt accumulated water, adopt the method that progressively improves salt marsh salinity water to tame, after a stage finishes, the complex microbial inoculum in upper stage is inoculated in the salt accumulated water of next high salt concentration, each salt accumulated water salt concn improves respectively 5% and 2%, so until 30% and 10% target salt concn, measure during this time COD clearance, experimental result as shown in Table 1 and Table 2, its principle is: Natrinema altunense sp and Facultative Halophiles can be using the high salt in edible mushrooms and organism as nutritive substance, the more growths of nutritive substance are better, increase along with salt concn, the middle microbes of composite fungus agent slowly adapts to new environment, and the microbes that can not adapt to high salt is dead, judgement composite bacteria can in high-salt wastewater, grow according to the changing conditions that is COD.COD is chemical oxygen demand (COD), and the higher explanation water body of COD value is subject to the more serious of Organic pollutants, and microorganism can reach the effect that reduces COD value by degradation of organic substances, with this, judges existing of microorganism.As can be seen from Table 1, along with the increase COD clearance of salt concn raises gradually, in salt concn, be that 20%-30% asks, COD clearance can reach 85%, and tends towards stability, and illustrates that the composite bacteria after domestication can be survived and have good degradation rate in high-salt wastewater.
Table 1 complex microbial inoculum I is with the changing conditions of the increase COD clearance of salt concn
| Salt concn (%) | 10-15 | 15-20 | 20-25 | 25-30 |
| COD clearance (%) | 88.6-93.4 | 84.5-93.8 | 81.2-84.6 | 82.3-86 |
Table 2 complex microbial inoculum II increases the changing conditions of COD clearance with salt concn
| Salt concn (%) | 1-2 | 2-4 | 4-6 | 6-8 | 8-10 |
| COD clearance (%) | 90.2-94.2 | 88.5-92.4 | 87.9-91.3 | 85.2-89.4 | 84.6-88.1 |
5, salt marsh water treatment
In the present embodiment, testing salt marsh water is the salt marsh water sample of 4 different areas.The quality of 4 salt marsh water samples is 5t, is divided into three steps and carries out desalting treatment.
The first step complex microbial inoculum I desalination: access the complex microbial inoculum I after high salt concentration domestication in the ratio of 5% (mass ratio) in salted Pleurotus eryngii salt accumulated water, aeration (oxygen supply) is cultivated 5d.Result is as shown in table 3.The present invention mainly utilizes microorganism the hobby physiological property of salt to be realized to the desalination degree of high-salt wastewater in conjunction with syllogic membrane technology, therefore mainly usings salt concn as observed value.Wherein adopt silver nitrate titration method to measure and reflection salt solution is processed the changing conditions of sodium-chlor in water later through composite fungus agent and film.As can be seen from Table 3, Pleurotus eryngii salt accumulated water is after the complex microbial inoculum I of the first step processes, and saltiness is 60% before processing, and the ratio of desalinization of the first step complex microbial inoculum I is 40%.
The sodium chloride content of table 3 Pleurotus eryngii bacterium salt accumulated water before and after the first step complex microbial inoculum I desalting treatment
| Sample number into spectrum | Salt concn (%) before processing | Salt concn (%) after processing |
| 1 | 23.8 | 14.56 |
[0043]
| 2 | 20.9 | 12.56 |
| 3 | 14.2 | 8.63 |
| 4 | 18.7 | 11.31 |
Second step syllogic film is processed desalination: this section of demineralising process adopts reverse osmosis membrane desalination, and technical process is as follows.
(1) features such as pre-treatment: the pre-treatment of reverse osmosis treatment equipment adopts traditional strainer filtering method, installs at salt marsh pond outlet the nylon filtering net that aperture is 5um, and nylon filtering net has corrosion-resistant, and easy cleaning, resistance are low.High-salt wastewater after strainer filtering can be removed large-scale suspended substance and colloid wherein, and reverse osmosis membrane processing is below had to provide protection.The complete high-salt wastewater of pre-treatment enters reverse osmosis membrane system.
(2) reverse osmosis: in order to obtain high decreasing ratio, this programme adopts one-level multi-stage type reverse-osmosis treated (Fig. 1), high salt accumulated water enters reverse osmosis system from the position of figure Central Plains water gauge will, then through high-pressure pump, send into first paragraph reverse osmosis membrane assembly, the concentrated solution obtaining passes through second segment reverse osmosis membrane assembly again, finally obtains the less salt accumulated water that saliferous rate is lower successively through syllogic desalting treatment.
(3) film cleans: with pump by clean, without the reverse osmosis water of free chlorine, from water reservoir, squeeze into the pressurized vessel of pressure pump and discharge several minutes, with clean water, in water reservoir, prepare afterwards scavenging solution, scavenging solution is circulated in pressurized vessel 1 hour.After cleaning completes, the water in emptying water reservoir also rinses, and then in water reservoir, fills it up with clean water in order to next step flushing.With pump by clean, without the water of free chlorine, from water reservoir, squeeze into pressurized vessel and discharge several minutes, after rinsing reverse osmosis system, under water reservoir bleed valve open mode, move reverse osmosis system 30 minutes, until finally cleaned, foamless water.
Result is as shown in table 4, and after syllogic film is processed, salt concn is 40% of the front salt concn of film processing substantially, i.e. the ratio of desalinization of reverse osmosis is 60%.The salt concn of the salt accumulated water after syllogic film is processed desalination is now only 5-6%, is the best salt concn of salt-durable microbe psychological need just, thereby enters the desalination stage of complex microbial inoculum II.
The sodium chloride content of table 4 Pleurotus eryngii salt accumulated water before and after second step syllogic film is processed
The 3rd step microbial composite fungus agent II desalination: by the complex microbial inoculum II of 5% (mass ratio) access after domestication, aeration (oxygen supply) is cultivated 4d in the salt accumulated water after syllogic film is processed.Result of study is as shown in table 5.Salt accumulated water saltiness after the 3rd step salt tolerant composite fungus agent is processed is 50% before processing, and the salt marsh water desalination rate after composite fungus agent II processes is 50%, and now, the salt concn of edible mushrooms salt accumulated water is in 2.5% left and right.
The sodium chloride content of table 5 Pleurotus eryngii salt accumulated water before and after the 3rd step microbial composite fungus agent II processes
| Sample number into spectrum | Salt concn (%) before processing | Salt concn (%) after processing |
| 1 | 5.86 | 2.45 |
| 2 | 5.10 | 2.54 |
| 3 | 3.45 | 1.62 |
| 4 | 4.48 | 2.20 |
Edible mushrooms salt marsh water demineralizing rate after comprehensive above three step desalting treatment can reach 97% left and right, the saltiness < 3% of salt accumulated water after desalination, this utilization of being rich in the low salt solution of nutraceutical edible mushrooms for the later stage provides condition, has also realized the process that industrial high-salt wastewater is turned waste into wealth simultaneously.Certainly, also can further adopt resin advanced desalination technology, thoroughly remove the salt in water, make ratio of desalinization reach 100%.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (3)
1. based on halophilic microorganism composite fungus agent and a syllogic membrane technique associated treatment edible mushrooms salt marsh water desalting method, it is characterized in that, its step is as follows:
(1) activation of bacterial classification
By bacterial classification Nesterenkonia halophile, Halomonas Xinjiangensis, Halogranumrubrum, Actinopolyspora halophila and Halopelagius inordinatus activation culture on activation medium respectively, soak time is 5d-7d; Wherein, described strain activation and culture based component is: casein hydrolyzate 7.5g, fish peptone 0.5g, yeast extract paste 3g, Trisodium Citrate 0.1g, sodium-chlor 150g, potassium primary phosphate 0.3g, magnesium sulfate 0.05g, Manganous chloride tetrahydrate 0.0001g, distilled water 1000mL, pH7.2;
T bacteria etragenococcus halophilus, Salimicrobium halophilum, Saccharomycescerevisiae, Bacillus subtilis and Weissella hellenica are activated respectively to 4d on activation medium; Wherein, described activation culture based component is: peptone 5g, yeast extract paste 3g, Trisodium Citrate 0.1g, casein hydrolysate 0.5g, sodium-chlor 50g, potassium primary phosphate 0.3g, magnesium sulfate 0.05g, distilled water 1000mL, pH7.2;
(2) cultivate altogether
By mass ratio mixing such as bacterial classification Nesterenkonia halophile, Halomonas Xinjiangensis, Halogranum rubrum, Actinopolyspora halophila and Halopelagius inordinatus after activation press, then add shaking table in the liquid nutrient medium that 100ml cultivates altogether to cultivate 4-5d, make complex microbial inoculum I; The composition of the described liquid nutrient medium of cultivating is altogether: peptone 10g, extractum carnis 3g, glucose 0.1g, yeast extract paste 0.2g, potassium primary phosphate 0.15g, Sodium.alpha.-ketopropionate 0.3g, magnesium sulfate 0.05g, iron protochloride 0.01g, calcium chloride 0.01g, sodium-chlor 150g, distilled water 1000ml, pH7.2; Adopt the described liquid nutrient medium of cultivating altogether and method to cultivate t bacteria etragenococcus halophilus, Salimicrobium halophilum, Saccharomyces cerevisiae, Bacillus subtilis and Weissellahellenica, wherein the consumption of sodium-chlor is adjusted into 50g, obtains complex microbial inoculum II;
(3) domestication of complex microbial inoculum
Complex microbial inoculum I after common cultivation and complex microbial inoculum II are put into respectively in edible mushrooms salt accumulated water, the salt concentration of initial salt accumulated water is respectively 10% and 1%, adopt the method that progressively improves salt marsh salinity water to tame, after a stage finishes, the complex microbial inoculum in upper stage is inoculated in the salt accumulated water of next high salt concentration, each salt accumulated water salt concn improves respectively 5% and 2%, so until 30% and 10% target salt concn;
(4) salt marsh water treatment
The desalination of the first step complex microbial inoculum I: the complex microbial inoculum I of access after high salt concentration domestication in edible mushrooms salt accumulated water, aeration is cultivated 5d;
Second step syllogic film is processed desalination: the salt accumulated water after the first step is processed, through the nylon filtering net pre-treatment of 5um, then adopts one-level multi-stage type reverse-osmosis treated;
The 3rd step microbial composite fungus agent II desalination: the complex microbial inoculum II of access after domestication in the salt accumulated water after syllogic film is processed, aeration is cultivated 4d.
2. desalting method according to claim 1, is characterized in that, the deposit number of described bacterial classification Nesterenkoniahalophile is KCTC19048; The deposit number of Halomonas Xinjiangensis is CCTCC AB208329; The deposit number of Halogranum rubrum is JCM15772; The deposit number of Actinopolyspora halophila is JCM3278; The deposit number of Halopelagius inordinatus is JCM15773; The deposit number of Tetragenococcus halophilus is CICC10286; The deposit number of Salimicrobium halophilum is CICC10482; The deposit number of Saccharomyces cerevisiae is CICC31443; The deposit number of Bacillus subtilis is CICC21377; The deposit number of Weissellahellenica is CGMCC1.2513.
3. desalting method according to claim 1, is characterized in that, in step (4), the inoculum size of complex microbial inoculum I is 5%wt; The inoculum size of complex microbial inoculum II is 5%wt.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105439380A (en) * | 2015-12-12 | 2016-03-30 | 常州大学 | Method for removing organic matters in salt-containing waste water |
| CN106746157A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of processing method of high slat-containing wastewater |
| CN109234214A (en) * | 2018-07-03 | 2019-01-18 | 宜兴市永洁环保设备有限公司 | A kind of production method of composite bacteria preparation and its application in sewage treatment |
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| WO2007120801A2 (en) * | 2006-04-13 | 2007-10-25 | Nereus Pharmaceuticals, Inc. | Fermentation method |
| CN103642737A (en) * | 2013-12-16 | 2014-03-19 | 西华大学 | Composite microbial agent capable of degrading organic matters in vegetable salinization water and desalinization water, as well as preparation method and application thereof |
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| WO2007120801A2 (en) * | 2006-04-13 | 2007-10-25 | Nereus Pharmaceuticals, Inc. | Fermentation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106746157A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of processing method of high slat-containing wastewater |
| CN106746157B (en) * | 2015-11-19 | 2020-10-16 | 中国石油化工股份有限公司 | Treatment method of high-salt-content wastewater |
| CN105439380A (en) * | 2015-12-12 | 2016-03-30 | 常州大学 | Method for removing organic matters in salt-containing waste water |
| CN109234214A (en) * | 2018-07-03 | 2019-01-18 | 宜兴市永洁环保设备有限公司 | A kind of production method of composite bacteria preparation and its application in sewage treatment |
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