CN112299562A - Method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion - Google Patents
Method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion Download PDFInfo
- Publication number
- CN112299562A CN112299562A CN201910691228.2A CN201910691228A CN112299562A CN 112299562 A CN112299562 A CN 112299562A CN 201910691228 A CN201910691228 A CN 201910691228A CN 112299562 A CN112299562 A CN 112299562A
- Authority
- CN
- China
- Prior art keywords
- microalgae
- culturing
- culture medium
- nitrogen
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion, which utilizes the yeast secretion as a growth stimulating factor of the microalgae so as to promote the capability of the microalgae to degrade the carbon, nitrogen and phosphorus. The invention promotes the synthesis and growth of protein of microalgae by an environment-friendly and green method, can more quickly increase the synthesis amount of protein of microalgae, improves the treatment efficiency of carbon, nitrogen and phosphorus in the culture wastewater, and has simple and easy operation method.
Description
Technical Field
The invention belongs to the technical field of wastewater reclamation and environmental protection, and particularly relates to a method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion.
Background
At present, the water environment pollution problem in China is widely concerned, and the problems of ammonia nitrogen, total nitrogen, organic matters and novel toxic pollutants are endless. The total nitrogen emission control requirement is implemented in the new 'ten water entries' of the country for coastal land level and above cities, so that the requirement for water treatment is improved by a higher level. The microalgae wastewater recycling technology can effectively solve the problem.
The microalgae can efficiently remove pollutants such as COD, nitrogen, phosphorus and the like in the water body under the heterotrophic-autotrophic mixed condition and absorb greenhouse gases; meanwhile, the algae can be recovered to obtain resources and energy sources with high added values such as grease, protein and the like. Many studies have shown that: the treatment technology is suitable for treating various kinds of culture wastewater, and is a novel potential biological treatment technology for recycling wastewater phosphorus. In the last 50 th century, microalgae have been used in sewage and wastewater treatment projects, and the microalgae technology has not become the mainstream sewage treatment technology due to limitations of treatment efficiency, technology and the like. In recent years, with the emergence of crisis, a novel sustainable sewage treatment system becomes a trigger for the development of microalgae technology, and the microalgae technology becomes a hot spot of the next generation of sewage treatment research again. The microalgae recycling technology represented by the chlorella pyrenoidosa can well meet the practical requirement.
The method is derived from the degradation process of various nitrogen-containing pollutants for the ammonia nitrogen which is a pollutant accounting for more than 60 percent of the total nitrogen of the water body. The microalgae represented by the chlorella can efficiently convert nitrogen pollutants in a water body into in-vivo proteins. Under autotrophic culture, the protein content of the chlorella accounts for 42% -58% of the dry weight of cells, and data show that the protein amino acid composition in the chlorella is higher than the protein content standard which is issued by the World Health Organization (WHO) and the Food and Agriculture Organization (FAO) of the United nations and is applied to human nutrition, and the high protein content and the high growth rate of the chlorella enable the protein in the chlorella to be called as a substance which has the best substitute for edible protein; it also has higher emulsification function and is a high-quality protein source; the protein of the chlorella also has other nutritive values, and the contained protein and polypeptide have the potential of resisting oxidation, improving the immunity of human bodies, resisting tumors, viruses, fungi and the like. Therefore, if a method for increasing the content of proteins in microalgae represented by chlorella is obtained, it is advantageous to improve the economy of microalgae wastewater treatment, improve the treatment efficiency, and advance a technology for recycling wastewater.
Disclosure of Invention
The invention aims to: provides a method for promoting microalgae to degrade carbon, nitrogen and phosphorus in wastewater.
In order to achieve the above object, the present invention provides a method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion, comprising the following steps:
s1, obtaining microalgae: purchasing microalgae or separating the microalgae from the culture wastewater, and culturing to obtain a microalgae culture;
s2, culturing yeast: selecting yeast, streaking on a sterilized YM solid culture medium plate, placing the plate in an illumination incubator, culturing for 7 days under the conditions of 30 ℃ and 2000Lux, transferring the plate onto an YM culture medium inclined plane, culturing for 5 days under the conditions of 30 ℃ and 2000Lux, transferring the plate into an M1 liquid culture medium for culturing for 2 days, and harvesting yeast culture after yeast growth is finished;
s3, obtaining yeast secretion: centrifuging or suction-filtering the yeast culture obtained in the step S2 to obtain yeast, then placing the obtained yeast in an M1 liquid culture medium with the volume of 1L, culturing for 2 days under the conditions of illumination all day and 25 ℃, measuring the removal rate of ammonia nitrogen and total nitrogen in the M1 culture medium, and finishing the culture when the removal rate reaches 70%; if the removal rate is lower than 70%, repeating the cultivation for 1-2 times under the conditions of illumination all day and 25 ℃, and then measuring the removal rate again until the removal rate reaches 70%; after the culture is finished, obtaining yeast secretion by centrifugation or suction filtration;
s4, strengthening microalgae: mixing and culturing the microalgae culture obtained in the step S1 and the yeast secretion obtained in the step S3 according to the volume ratio of 6: 1-1: 1 to obtain strengthened microalgae;
s5, putting the strengthened microalgae obtained in the S4 into culture wastewater, and culturing for 4-7 days to promote degradation of carbon, nitrogen and phosphorus;
wherein each liter of the M1 liquid culture medium comprises the following components: CH (CH)3COONa 3.68g，Na2HPO4 28.73mg， NH4Cl 57.27mg，CaCl2.2H2O17.2 mg, pH adjusted to 7.0 with NaOH.
As a preferred embodiment of the method of the present invention, in S1, the method for purchasing microalgae and the culturing comprises: adding microalgae into an M0 liquid culture medium, culturing for 2-3 days under the conditions of 25 ℃ and 5500-6000 Lux, and shaking for 2-3 times every day to obtain a microalgae culture;
wherein each liter of the M0 liquid culture medium comprises the following components: NaNO3 1500mg，K2HPO4 0.04mg， MgSO47H2O 75mg，CaCl22H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO3 20mg。
As a preferred technical solution of the method of the present invention, in S1, the method for separating microalgae from aquaculture wastewater and culturing comprises: taking 10-20L of aquaculture wastewater, rough filtering, centrifuging, removing suspended matters and large-scale plankton, then performing aeration and precipitation, removing the precipitate, performing suction filtration again, collecting the precipitate on a filter membrane, transferring the precipitate into an M0 liquid culture medium, culturing for 2-3 days under the condition of 25 ℃ 4000-5000 Lux full-day illumination, shaking for 2-3 times every day to obtain a primary culture, then inoculating the primary culture into an M0 liquid culture medium, culturing for 7 days under the conditions of 25 ℃ full-day illumination and 150rpm, measuring the removal rates of nitrate, total nitrogen and total phosphorus every day, replacing the M0 liquid culture medium when the removal rates respectively reach 50%, 40% and 40%, and obtaining the primary culture after the removal effects of the nitrate, the total nitrogen and the total phosphorus respectively reach 80%, 50% and 60%; diluting the primary culture, finding microalgae cells under a low magnification magnifier, sucking out, centrifuging at 5000 rpm, inoculating the obtained precipitate to M0 solid culture medium, and streaking for separation to obtain microalgae; the step of separating the microalgae from the culture wastewater can be repeated for 2-3 times, so that sufficient microalgae can be obtained. And then adding the microalgae into an M0 liquid culture medium, culturing for 2-3 days under the conditions of 25 ℃ and 5500-6000 Lux, and shaking for 2-3 times every day to obtain a microalgae culture.
Wherein each liter of the M0 liquid culture medium comprises the following components: NaNO3 1500mg，K2HPO4 0.04mg， MgSO47H2O 75mg，CaCl22H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO3 20mg；
The M0 solid culture medium comprises the following components per liter: NaNO3 1500mg，K2HPO4 0.04mg，MgSO47H2O 75mg/L，CaCl22H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO320mg, 2 wt% agar powder.
As a preferred embodiment of the method of the present invention, the purchased microalgae is Chlorella pyrenoidosa (Chlorella pyrenoidosa) or Chlorella pyrenoidosa.
As a preferable technical scheme of the method, in S3, the rotating speed of the centrifugation is not lower than 10000rpm, and the aperture of the suction filtration is not less than 0.22 μm.
Compared with the prior art, the invention has the following beneficial effects:
the invention promotes the synthesis and growth of protein of microalgae by an environment-friendly and green method, improves the degradation efficiency of microalgae on carbon, nitrogen and phosphorus in the culture wastewater, and has simple and easy operation method.
Drawings
FIG. 1 shows the result of the effect of yeast secretion in promoting the removal of COD from chlorella;
FIG. 2 shows the result of the effect of yeast secretion in promoting the removal of total nitrogen from chlorella;
FIG. 3 shows the result of the effect of yeast secretion in promoting the removal of total phosphorus from chlorella.
FIG. 4 shows that the secretion of test yeast promotes the average removal rate of COD, total nitrogen and total phosphorus in chlorella.
Detailed Description
In order to make the objects, technical solutions and advantageous technical effects of the present invention clearer, the present invention is further described in detail with reference to the following embodiments. It should be understood that the embodiments described in this specification are only for the purpose of illustrating the invention and are not to be construed as limiting the invention, and the parameters, proportions and the like of the embodiments may be suitably selected without materially affecting the results.
The culture media and the specific formulations used in the following examples are as follows:
m1 liquid medium per liter included: CH (CH)3COONa 3.68g，Na2HPO4 28.73mg，NH4Cl 57.27mg， CaCl2.2H2O17.2 mg, pH adjusted to 7.0 with NaOH.
M0 liquid medium per liter included: NaNO3 1500mg，K2HPO4 0.04mg，MgSO47H2O 75mg，CaCl22H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO3 20mg。
The M0 solid culture medium comprises the following components in each liter: NaNO3 1500mg，K2HPO4 0.04mg，MgSO47H2O 75mg/L，CaCl22H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO320mg, 2 wt% agar powder.
The remaining medium is a medium commonly used in the art.
The strain activation and culture method is a conventional method in the art, unless otherwise specified.
Example 1
1. Taking 30L of wastewater from a waterfowl breeding wastewater factory, removing suspended matters, large-scale plankton and oil stains in the wastewater after conventional isolation, then carrying out closed aeration for 30min, precipitating for 30min, and circulating for 2-3 times for later use.
2. Obtaining and culturing microalgae: obtaining concentrated solution through suction filtration, and collecting precipitate on a filter membrane; washing the precipitate with sterile water for 1-2 times, transferring to an M0 liquid culture medium, culturing for 3 days at 25 ℃ under the condition of illumination of 4000-5000 Lux all day, and shaking for 3 times every day to obtain a primary culture; inoculating the primary culture in an M0 liquid culture medium, culturing under the conditions of illumination all day and 25 ℃ at 150rpm, measuring the removal efficiency of nitrate, total nitrogen and total phosphorus every day, and replacing the culture medium M0 after the removal efficiency of nitrate, total nitrogen and total phosphorus respectively reaches 50%, 40% and 40%, until the removal efficiency of nitrate, total nitrogen and total phosphorus respectively reaches 80%, 50% and 60%, so as to obtain a primary culture; diluting the primary culture, finding chlorella cells in a low magnification magnifier, sucking out, centrifuging at 5000 r/min, collecting precipitate, inoculating to M0 solid culture medium, and streaking, purifying and separating to obtain chlorella strain as wastewater. This step can be repeated 2-3 times. And then adding the wastewater chlorella strain into an M0 liquid culture medium, culturing for 2-3 days under the conditions of 25 ℃ and 5500-6000 Lux, and shaking for 2-3 times every day to obtain a chlorella culture.
3. The yeast is purchased from a standard strain bank by a purchasing method, and the strain is rhodotorula toruloides (Rhodosporidium toruloides). Yeast secretion was obtained as follows. Firstly, after the strain is activated and cultured, the yeast is harvested under the centrifugation condition of 10000 r/rpm, and the supernatant is removed. Then, the obtained yeast was placed in a 1L volume of M1 liquid medium, and cultured for 2 days under the conditions of light irradiation and 25 ℃ throughout the day, and the residual nitrogen in the supernatant M1 medium was measured, whereby the ammonia nitrogen removal rate was 79.5%, the total nitrogen removal rate was 75.1%, and the yeast culture was completed when the 70% standard was reached. After the culture is finished, the filtrate obtained by 0.22um suction filtration is the yeast secretion which needs to be used within 12-24 hours.
4. And (4) treating the wastewater. Mixing the obtained yeast secretion and chlorella culture according to the ratio of 1: 3 inoculating into a 5L sequencing batch reactor, and inoculating the treated aquaculture wastewater, wherein the pollutant concentrations are respectively as follows: 45-50mg/L of total nitrogen, 30-35mg/L of ammonia nitrogen, 36-40mg/L of total phosphorus, 800-1000mg/L of COD and 6-7 of pH. In the process of treating wastewater, only adding chlorella group, yeast secretion and chlorella control group,
after 7-day sequencing batch operation, the removal efficiency of COD, ammonia nitrogen, total nitrogen, phosphate and total phosphorus was compared, and the results are shown in FIGS. 1 to 3.
Example 2
1. Taking 20L of wastewater from a pig raising wastewater factory, removing suspended matters, large plankton and oil stains in the wastewater after conventional isolation, performing closed aeration for 25min, precipitating for 30min, and circulating for 2-3 times for later use.
2. Obtaining and culturing microalgae: the chlorella is chlorella pyrenoidosa purchased from a standard strain bank, and is transferred to an M0 liquid culture medium after being activated, the chlorella pyrenoidosa is cultured for 7 days under the conditions of illumination of the whole day, 25 ℃ and 150rpm, the removal efficiency of nitrate, total nitrogen and total phosphorus is measured every day, the culture medium M0 is replaced after the removal efficiency of the nitrate, the total nitrogen and the total phosphorus respectively reaches 50 percent, 40 percent and 40 percent, and a primary culture is obtained until the removal efficiency of the nitrate, the total nitrogen and the total phosphorus respectively reaches 80 percent, 50 percent and 60 percent; diluting the primary culture, finding chlorella cells in a low magnification magnifier, sucking out, centrifuging at 5000 r/min, collecting precipitate, inoculating to M0 solid culture medium, and streaking, purifying and separating to obtain chlorella strain as wastewater. This step can be repeated 2-3 times. And then adding the wastewater chlorella strain into an M0 liquid culture medium, culturing for 2-3 days under the conditions of 25 ℃ and 5500-6000 Lux, and shaking for 2-3 times every day to obtain a chlorella culture.
3. Yeast was Rhodotorula glutinis, a commercially available strain of Rhodotorula glutinis, obtained from a stock of standard strains, and was processed in the same manner as in example 1.
4. And (4) treating the wastewater. The obtained chlorella and yeast secretion are respectively put into the culture wastewater according to the proportion of 3:1 and 6:1 for use. The pollutant concentration of the aquaculture wastewater is respectively as follows: 35-40mg/L of total nitrogen, 30-35mg/L of ammonia nitrogen, 40mg/L of total phosphorus, 1200mg/L of COD (chemical oxygen demand) and 6-7 of pH. (the treatment effect is related to the purchased microalgae, the experiment is repeated for 5 times, the COD removal rate is 3:1, the TN and TP removal rates of the groups are the highest, the average removal rate is 79.8 percent and 86.3 percent respectively, the COD removal rates are close to each other, the 6:1 group is 86.8 percent, and the 3:1 group is 83.6 percent.
Appropriate changes and modifications to the embodiments described above will become apparent to those skilled in the art from the disclosure and teachings of the foregoing description. Therefore, the present invention is not limited to the specific embodiments disclosed and described above, and some modifications and variations of the present invention should fall within the scope of the claims of the present invention. Furthermore, although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.
Claims (5)
1. A method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion is characterized by comprising the following steps:
s1, obtaining microalgae: purchasing microalgae or separating the microalgae from the culture wastewater, and culturing to obtain a microalgae culture;
s2, culturing yeast: selecting yeast, streaking on a sterilized YM solid culture medium plate, placing the plate in an illumination incubator, culturing for 7 days under the conditions of 30 ℃ and 2000Lux, transferring the plate onto an YM culture medium inclined plane, culturing for 5 days under the conditions of 30 ℃ and 2000Lux, transferring the plate into an M1 liquid culture medium for culturing for 2 days, and harvesting yeast culture after yeast growth is finished;
s3, obtaining yeast secretion: centrifuging or suction-filtering the yeast culture obtained in the step S2 to obtain yeast, then placing the obtained yeast in an M1 liquid culture medium with the volume of 1L, culturing for 2 days under the conditions of illumination all day and 25 ℃, measuring the removal rate of ammonia nitrogen and total nitrogen in the M1 culture medium, and finishing the culture when the removal rate reaches 70%; if the removal rate is lower than 70%, repeating the cultivation for 1-2 times under the conditions of illumination all day and 25 ℃, and then measuring the removal rate again until the removal rate reaches 70%; after the culture is finished, obtaining yeast secretion by centrifugation or suction filtration;
s4, strengthening microalgae: mixing the microalgae culture obtained in the step S1 and the yeast secretion obtained in the step S3 according to the volume ratio of 6: 1-1: 1, and culturing for 5 days in an M1 liquid culture medium under the conditions of 25 ℃ and 5500-6000 Lux to obtain strengthened microalgae;
s5, putting the strengthened microalgae obtained in the S4 into culture wastewater, and culturing for 4-7 days to promote degradation of carbon, nitrogen and phosphorus;
wherein each liter of the M1 liquid culture medium comprises the following components: CH (CH)3COONa 3.68g，Na2HPO4 28.73mg，NH4Cl 57.27mg，CaCl2.2H2O17.2 mg, pH adjusted to 7.0 with NaOH.
2. The method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion as claimed in claim 1, wherein in S1, the method for purchasing microalgae and the method for culturing microalgae are as follows: adding microalgae into an M0 liquid culture medium, culturing for 2-3 days under the conditions of 25 ℃ and 5500-6000 Lux, and shaking for 2-3 times every day to obtain a microalgae culture;
wherein each liter of the M0 liquid culture medium comprises the following components: NaNO3 1500mg，K2HPO4 0.04mg，MgSO47H2O 75mg，CaCl22H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO3 20mg。
3. The method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion as claimed in claim 1, wherein in S1, the method for separating microalgae from aquaculture wastewater and culturing comprises the following steps: taking 10-20L of aquaculture wastewater, rough filtering, centrifuging, removing suspended matters and large-scale plankton, then performing aeration and precipitation, removing the precipitate, performing suction filtration again, collecting the precipitate on a filter membrane, transferring the precipitate into an M0 liquid culture medium, culturing for 2-3 days under the conditions of 25 ℃ and 4000-5000 Lux all day illumination, shaking for 2-3 times every day to obtain a primary culture, then inoculating the primary culture into an M0 liquid culture medium, culturing for 7 days under the conditions of 25 ℃ and all day illumination and 150rpm, measuring the removal rates of nitrate, total nitrogen and total phosphorus every day, replacing the M0 liquid culture medium when the removal rates respectively reach 50%, 40% and 40%, and obtaining a primary culture after the removal effects of the nitrate, the total nitrogen and the total phosphorus respectively reach 80%, 50% and 60%; diluting the primary culture, finding microalgae cells under a low magnification magnifier, sucking out, centrifuging at 5000 rpm, inoculating the obtained precipitate to M0 solid culture medium, and streaking for separation to obtain microalgae; then adding the microalgae into an M0 liquid culture medium, culturing for 2-3 days under the conditions of 25 ℃ and 5500-6000 Lux, and shaking for 2-3 times every day to obtain a microalgae culture;
wherein each liter of the M0 liquid culture medium comprises the following components: NaNO3 1500mg，K2HPO4 0.04mg，MgSO47H2O 75mg，CaCl22H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO3 20mg；
The M0 solid culture medium comprises the following components per liter: NaNO3 1500mg，K2HPO4 0.04mg，MgSO47H2O 75mg/L，CaCl22H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO320mg, 2 wt% agar powder.
4. The method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion as claimed in claim 1, wherein the purchased microalgae is Chlorella pyrenoidosa (Chlorella pyrenoidosa) or Chlorella pyrenoidosa (Chlorella pyrenoidosa).
5. The method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion as claimed in claim 1, wherein in S3, the rotation speed of the centrifugation is not lower than 10000rpm, and the aperture of the suction filtration is not less than 0.22 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910691228.2A CN112299562A (en) | 2019-07-29 | 2019-07-29 | Method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910691228.2A CN112299562A (en) | 2019-07-29 | 2019-07-29 | Method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112299562A true CN112299562A (en) | 2021-02-02 |
Family
ID=74328973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910691228.2A Pending CN112299562A (en) | 2019-07-29 | 2019-07-29 | Method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112299562A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114231454A (en) * | 2021-12-21 | 2022-03-25 | 北京天诚众合科技发展有限公司 | Bacteria-algae composition for degrading water body and directionally eliminating blue algae, production process and device |
| CN115558623A (en) * | 2022-11-07 | 2023-01-03 | 宁夏农林科学院农业资源与环境研究所（宁夏土壤与植物营养重点实验室） | Preparation method of microbial agent and organic fertilizer for treating cow breeding wastewater |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6443182A (en) * | 1987-08-10 | 1989-02-15 | Osaka Seiken Kk | Mixed cultured product, production and method thereof |
| US20130078686A1 (en) * | 2011-09-27 | 2013-03-28 | Synthetic Genomics, Inc. | Production and secretion of fatty acids and fatty acid derivatives |
| CN104212720A (en) * | 2014-09-04 | 2014-12-17 | 山东大学 | Method for producing microalgae biomass by using monosodium glutamate ion-exchanged mother liquid |
| CN105441524A (en) * | 2016-01-27 | 2016-03-30 | 扬州大学 | Method for increasing yield of microalgae grease with saccharose as carbon source through co-culture |
| CN106929422A (en) * | 2017-03-15 | 2017-07-07 | 华南理工大学 | A kind of method that chlorella and yeast co-culture purification yeast wastewater |
| CN108624506A (en) * | 2018-05-16 | 2018-10-09 | 华南理工大学 | The method of microalgae and yeast mixed culture purification biogas slurry coproduction microbial biomass |
| CN109536388A (en) * | 2018-11-26 | 2019-03-29 | 江苏科技大学 | A method of micro algae growth speed and protein content are improved using silk reeling wastewater |
-
2019
- 2019-07-29 CN CN201910691228.2A patent/CN112299562A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6443182A (en) * | 1987-08-10 | 1989-02-15 | Osaka Seiken Kk | Mixed cultured product, production and method thereof |
| US20130078686A1 (en) * | 2011-09-27 | 2013-03-28 | Synthetic Genomics, Inc. | Production and secretion of fatty acids and fatty acid derivatives |
| CN104212720A (en) * | 2014-09-04 | 2014-12-17 | 山东大学 | Method for producing microalgae biomass by using monosodium glutamate ion-exchanged mother liquid |
| CN105441524A (en) * | 2016-01-27 | 2016-03-30 | 扬州大学 | Method for increasing yield of microalgae grease with saccharose as carbon source through co-culture |
| CN106929422A (en) * | 2017-03-15 | 2017-07-07 | 华南理工大学 | A kind of method that chlorella and yeast co-culture purification yeast wastewater |
| CN108624506A (en) * | 2018-05-16 | 2018-10-09 | 华南理工大学 | The method of microalgae and yeast mixed culture purification biogas slurry coproduction microbial biomass |
| CN109536388A (en) * | 2018-11-26 | 2019-03-29 | 江苏科技大学 | A method of micro algae growth speed and protein content are improved using silk reeling wastewater |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114231454A (en) * | 2021-12-21 | 2022-03-25 | 北京天诚众合科技发展有限公司 | Bacteria-algae composition for degrading water body and directionally eliminating blue algae, production process and device |
| CN115558623A (en) * | 2022-11-07 | 2023-01-03 | 宁夏农林科学院农业资源与环境研究所（宁夏土壤与植物营养重点实验室） | Preparation method of microbial agent and organic fertilizer for treating cow breeding wastewater |
| CN115558623B (en) * | 2022-11-07 | 2023-10-17 | 宁夏农林科学院农业资源与环境研究所（宁夏土壤与植物营养重点实验室） | Preparation method of microbial agent and organic fertilizer for treating cow breeding wastewater |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107082489B (en) | Method for synchronously removing manganese and nitrate in underground water | |
| CN108587915B (en) | Chlorella W5 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN108546649B (en) | Chlorella W2 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN110577336B (en) | Harmless treatment method for enhancing flocculation of aquaculture tail water | |
| CN104628225A (en) | Treatment method of ammonia-nitrogen-containing industrial wastewater | |
| CN105540870A (en) | Treatment composition for lakes and rivers | |
| CN114105307A (en) | Method for removing Cr in wastewater by using living heterotrophic microalgae6+Method (2) | |
| CN107673483B (en) | Biological treatment method for copper-polluted seawater | |
| CN109576159B (en) | Chlorella W4 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN112299562A (en) | Method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion | |
| CN111977800A (en) | Method for treating sewage by using immobilized chlorella and rhodotorula benthamii and application | |
| CN109081447B (en) | Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella, acinetobacter and pseudomonas | |
| CN112028249A (en) | Method for promoting formation of root surface iron film and accelerating removal of sediment pollutants by utilizing sediment surface micro-aeration | |
| Shen et al. | Composition of extracellular and intracellular polymeric substances produced by Scenedesmus and Microcystis | |
| CN109576160B (en) | Chlorella W3 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN111690546B (en) | Rhodosporidium toruloides ZDFY1801 and application thereof | |
| CN108164086B (en) | Breeding wastewater recycling system and application thereof in recycling breeding wastewater | |
| CN102294227B (en) | Preparation method and application method of chlorella seawater purifying biological adsorbent | |
| CN114292797B (en) | Physarum viscosum and application of microbial flocculant thereof in sewage treatment | |
| CN110498514A (en) | A method of purification waste water | |
| CN112978888B (en) | Method for treating aquaculture tail water | |
| CN109110927B (en) | Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella and acinetobacter | |
| CN112300941A (en) | Method for promoting microalgae growth and enhancing microalgae protein accumulation by using yeast secretion | |
| CN108865893B (en) | Method for harvesting and circularly culturing microalgae through alkaline flocculation | |
| CN113526801A (en) | Wood vinegar and biomass charcoal combined sewage treatment method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210202 |
|
| RJ01 | Rejection of invention patent application after publication |