CN115558623B - Preparation method of microbial agent and organic fertilizer for treating cow breeding wastewater - Google Patents

Preparation method of microbial agent and organic fertilizer for treating cow breeding wastewater Download PDF

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CN115558623B
CN115558623B CN202211390528.5A CN202211390528A CN115558623B CN 115558623 B CN115558623 B CN 115558623B CN 202211390528 A CN202211390528 A CN 202211390528A CN 115558623 B CN115558623 B CN 115558623B
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fermentation
bacillus
organic fertilizer
ammonia nitrogen
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CN115558623A (en
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郭鑫年
许泽华
李百云
邢润东
周涛
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Agricultural Resource And Environment Institute Ningxia Academy Of Agricultural And Forestry Sciences (ningxia Soil And Plant Nutrition Key Laboratory)
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Abstract

The invention relates to the technical field of cultivation sewage treatment, in particular to a preparation method of a microbial agent and an organic fertilizer for treating cow cultivation wastewater. The microbial agent for treating the cow breeding wastewater is prepared from the following components in parts by mass: 12-18 parts of solid fermentation microbial inoculum, 10-20 parts of liquid fermentation microbial inoculum and 10-20 parts of ammonia nitrogen remover. The microbial agent disclosed by the invention not only can effectively carry out innocent treatment on the cow breeding wastewater, but also can change the cow breeding wastewater into valuable substances through a fermentation process, so that the microbial agent becomes an organic fertilizer with excellent effect, not only solves the problem that the cow breeding wastewater is difficult to treat, but also improves the value of the cow breeding wastewater.

Description

Preparation method of microbial agent and organic fertilizer for treating cow breeding wastewater
Technical Field
The invention relates to the technical field of cultivation sewage treatment, in particular to a preparation method of a microbial agent and an organic fertilizer for treating cow cultivation wastewater.
Background
Along with the improvement of the living standard of people, the demand of milk products is also continuously increased, but a large amount of waste water, such as cow dung and urine, cowshed flushing water, milking parlor flushing water, factory living sewage, silage leachate, boiler drainage and the like, is generated in the cow breeding process, and the waste water contains ultrahigh ammonia nitrogen, COD, total phosphorus and the like, and can pollute the environment when being discharged or treated at will without reaching the standard.
The cow breeding wastewater mainly has the following characteristics: contains solid waste (such as feces, forage, etc.), organic matter, nitrogen and phosphorus, and has high water content and low temperature. The uniqueness of the cow breeding wastewater increases the treatment difficulty, and the treatment is difficult to reach the standard by using the existing treatment method. The current treatment methods of the dairy waste water mainly comprise an anaerobic treatment method, an aerobic treatment method and an anaerobic-aerobic combined treatment method. Although the anaerobic treatment method can treat the wastewater of the dairy farm with high concentration, the concentration of effluent water after anaerobic treatment is still high, the effluent water can not reach the standard and is discharged, and the effluent water is accompanied with odor and affects the surrounding environment.
Because the wastewater in the dairy farm has the characteristics of high organic matters, high nitrogen and phosphorus and the like, the aerobic treatment technology mainly aims at the wastewater with COD lower than 500mg/L, so that the wastewater in the dairy farm is diluted before being treated by the aerobic treatment method, the water demand is high, the treatment capacity is increased, and the treatment cost is increased. At present, one of the most utilized methods is an anaerobic-aerobic combined treatment method, but the treatment effect of the method on nitrogen and phosphorus in the dairy farm wastewater is poor, and the main reason is that most organic matters are consumed in an anaerobic stage, so that the denitrification effect is influenced.
Therefore, how to provide a treatment method of cow breeding wastewater and prepare the cow breeding wastewater into fertilizer for crop growth, so as to solve the technical problems of complex treatment method, high cost and poor treatment effect of the cow breeding wastewater in the prior art, and the method is a problem to be solved by the technicians in the field.
Disclosure of Invention
The invention aims to provide a preparation method of a microbial agent and an organic fertilizer for treating cow breeding wastewater, which solves the technical problems of complex treatment method, high cost and poor treatment effect of the cow breeding wastewater in the prior art.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a microbial agent for treating cow breeding wastewater, which is prepared from the following components in parts by weight: 12-18 parts of solid fermentation microbial inoculum, 10-20 parts of liquid fermentation microbial inoculum and 10-20 parts of ammonia nitrogen remover;
the solid fermentation inoculant is bacillus brevis, lactobacillus, bacillus megatherium and saccharomycetes;
the liquid fermentation inoculant is bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomyces;
the ammonia nitrogen remover is chlorella, blue algae, candida tropicalis and photosynthetic bacteria.
Preferably, the mass ratio of the bacillus brevis, the lactobacillus, the bacillus megaterium and the saccharomycetes is 8-12:8-12:12-18:18-26;
the mass ratio of the bacillus thuringiensis to the bacillus licheniformis to the bacillus subtilis to the trichoderma viride to the aspergillus niger to the streptococcus thermophilus to the actinomycetes is 12-16:20-24:13-19:8-12:14-18:4-8:3-9;
the mass ratio of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria is 22-28:13-19:11-17:4-12;
the concentration of the bacteria of the Brevibacterium, the lactobacillus, the bacillus megaterium and the saccharomycetes in the solid fermentation microbial inoculum is independently 2-6 hundred million/ml;
the bacterial concentration of bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomycetes in the liquid fermentation microbial inoculum is independently 1-4 hundred million/ml;
the bacteria concentration of chlorella, blue algae, candida tropicalis and photosynthetic bacteria in the ammonia nitrogen remover is independently 2-6 hundred million/ml.
Preferably, the preparation method of the microbial agent comprises the following steps:
(1) Mixing Brevibacterium, lactobacillus, bacillus megatherium and saccharomycetes to obtain a solid fermentation microbial inoculum;
(2) Mixing bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomycetes to obtain a liquid fermentation microbial inoculum;
(3) Mixing chlorella, blue algae, candida tropicalis and photosynthetic bacteria to obtain the ammonia nitrogen remover.
The invention also provides a preparation method of the organic fertilizer, which comprises the following steps:
(S1) performing solid-liquid separation on cow breeding wastewater to obtain filter residues and filtrate;
(S2) mixing the filter residue obtained in the step (S1) with the solid fermentation microbial inoculum for solid fermentation to obtain a solid organic fertilizer;
(S3) mixing the filtrate obtained in the step (S1) with the liquid fermentation microbial inoculum for primary fermentation to obtain fermentation liquor;
and (S4) mixing the fermentation liquor obtained in the step (S3) with an ammonia nitrogen remover for secondary fermentation to obtain the liquid organic fertilizer.
Preferably, in the step (S2), the mass ratio of the filter residue to the solid fermentation inoculant is 80-100: 6 to 10.
Preferably, the solid fermentation in step (S2) is performed for 7 to 15 days.
Preferably, the temperature of the primary fermentation in the step (S3) is 32-36 ℃; the time of the primary fermentation is 4-8 d.
Preferably, the temperature of the secondary fermentation in the step (S4) is 24-28 ℃; the secondary fermentation time is 25-35 h.
Preferably, the mass ratio of the filtrate in the step (S3) to the liquid fermentation inoculant is 80-100: 4 to 8.
Preferably, in the step (S4), the mass ratio of the fermentation liquid to the ammonia nitrogen remover is 80-100: 2 to 6.
Compared with the prior art, the invention has the following beneficial effects:
1. the microbial agent disclosed by the invention not only can effectively carry out innocent treatment on the cow breeding wastewater, but also can change the cow breeding wastewater into valuable substances through a fermentation process, so that the microbial agent becomes an organic fertilizer with excellent effect. Not only solves the problem that the cow breeding wastewater is difficult to treat, but also obviously improves the value of the cow breeding wastewater.
2. The microbial agent is mainly divided into three parts, wherein the solid fermentation microbial agent and filter residues are mixed and fermented to obtain the solid organic fertilizer which can be used as a base fertilizer for planting crops.
3. In the prior art, most of the filtrate of the cultivation wastewater is treated as biogas slurry to generate biogas for people to use, and the added liquid fermentation microbial inoculum and the separated filtrate are mixed and fermented to obtain the liquid organic fertilizer which can be used as topdressing of crops and applied along with water, thereby providing a new scheme for the treatment of the filtrate. However, the ammonia nitrogen content in the filtrate is high, and ammonia nitrogen in the filtrate cannot be effectively removed by simple fermentation.
Detailed Description
The invention provides a microbial agent for treating cow breeding wastewater, which is prepared from the following components in parts by weight: 12-18 parts of solid fermentation microbial inoculum, 10-20 parts of liquid fermentation microbial inoculum and 10-20 parts of ammonia nitrogen remover; the preparation method is preferably prepared from the following components in parts by weight: 13-17 parts of solid fermentation microbial inoculum, 12-18 parts of liquid fermentation microbial inoculum and 12-18 parts of ammonia nitrogen remover; the preparation method is further preferably prepared from the following components in parts by weight: 14-16 parts of solid fermentation microbial inoculum, 14-16 parts of liquid fermentation microbial inoculum and 14-16 parts of ammonia nitrogen remover; more preferably, the composition is prepared from the following components in parts by weight: 15 parts of solid fermentation microbial inoculum, 15 parts of liquid fermentation microbial inoculum and 15 parts of ammonia nitrogen remover.
In the invention, the solid fermentation inoculant is Brevibacterium, lactobacillus, bacillus megaterium and saccharomycetes;
the liquid fermentation inoculant is bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomyces;
the ammonia nitrogen remover is chlorella, blue algae, candida tropicalis and photosynthetic bacteria.
In the invention, the mass ratio of the bacillus brevis, the lactobacillus, the bacillus megaterium and the saccharomycetes is 8-12:8-12:12-18:18-26; preferably 9-11:9-11:13-17:19-25; further preferably 10:10:14 to 16:20 to 24; more preferably 10:10:15:22.
In the invention, the mass ratio of the bacillus thuringiensis, the bacillus licheniformis, the bacillus subtilis, the trichoderma viride, the aspergillus niger, the streptococcus thermophilus and the actinomycetes is 12-16:20-24:13-19:8-12:14-18:4-8:3-9; preferably 13-15:21-23:14-18:9-11:15-17:5-7:4-8; further preferably 14:22:15 to 17:10:16:5 to 7:5 to 7; more preferably 14:22:16:10:16:6:6.
In the invention, the mass ratio of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria is 22-28:13-19:11-17:4-12; preferably 23-27:14-18:12-16:6-10; further preferably 24-26:15-17:13-15:7-9; more preferably 25:16:14:8.
In the invention, the concentration of the bacillus brevis, the lactobacillus, the bacillus megatherium and the saccharomycetes in the solid fermentation inoculant is independently 2-6 hundred million/ml; preferably 3 to 5 hundred million/ml; further preferably 4 hundred million/ml.
In the invention, the bacterial concentration of bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomycetes in the liquid fermentation inoculant is independently 1-4 hundred million/ml; preferably 2 to 3 hundred million/ml; further preferably 2.5 hundred million/ml.
In the invention, the concentration of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria in the ammonia nitrogen remover is independently 2-6 hundred million/ml; preferably 3 to 5 hundred million/ml; further preferably 4 hundred million/ml.
In the invention, the preparation method of the microbial agent comprises the following steps:
(1) Mixing Brevibacterium, lactobacillus, bacillus megatherium and saccharomycetes to obtain a solid fermentation microbial inoculum;
(2) Mixing bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomycetes to obtain a liquid fermentation microbial inoculum;
(3) Mixing chlorella, blue algae, candida tropicalis and photosynthetic bacteria to obtain the ammonia nitrogen remover.
The invention also provides a preparation method of the organic fertilizer, which comprises the following steps:
(S1) performing solid-liquid separation on cow breeding wastewater to obtain filter residues and filtrate;
(S2) mixing the filter residue obtained in the step (S1) with the solid fermentation microbial inoculum for solid fermentation to obtain a solid organic fertilizer;
(S3) mixing the filtrate obtained in the step (S1) with the liquid fermentation microbial inoculum for primary fermentation to obtain fermentation liquor;
and (S4) mixing the fermentation liquor obtained in the step (S3) with an ammonia nitrogen remover for secondary fermentation to obtain the liquid organic fertilizer.
In the invention, the mass ratio of the filter residue to the solid fermentation inoculant in the step (S2) is 80-100: 6-10; preferably 84 to 96: 7-9; further preferably 88 to 92:8, 8; more preferably 90:8.
in the invention, the solid fermentation time in the step (S2) is 7-15 d; preferably 8 to 14d; further preferably 9 to 13d; more preferably 11d.
In the invention, the temperature of the primary fermentation in the step (S3) is 32-36 ℃; preferably 33-35 ℃; further preferably 34 ℃.
In the invention, the time of the primary fermentation is 4-8 d; preferably 5 to 7 days; further preferably 6d.
In the invention, the temperature of the secondary fermentation in the step (S4) is 24-28 ℃; preferably 25 to 27 ℃; further preferably 26 ℃.
In the invention, the secondary fermentation time is 25-35 h; preferably 27 to 33 hours; further preferably 29 to 31 hours; more preferably 30h.
In the invention, the mass ratio of the filtrate to the liquid fermentation inoculant in the step (S3) is 80-100: 4 to 8; preferably 84 to 96:5 to 7; further preferably 88 to 92:6, preparing a base material; more preferably 90:6.
in the invention, the mass ratio of the fermentation liquid to the ammonia nitrogen remover in the step (S4) is 80-100: 2 to 6; preferably 84 to 96:3 to 5; further preferably 88 to 92:4, a step of; more preferably 90:4.
the technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The microbial agent for treating the cow breeding wastewater is prepared from the following components in parts by weight: 12 parts of solid fermentation bacteria, 10 parts of liquid fermentation bacteria and 10 parts of ammonia nitrogen remover;
the solid fermentation inoculant is bacillus brevis, lactobacillus, bacillus megatherium and saccharomycetes;
the liquid fermentation inoculant is bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomyces;
the ammonia nitrogen remover is chlorella, blue algae, candida tropicalis and photosynthetic bacteria;
the mass ratio of the bacillus brevis to the lactobacillus to the bacillus megaterium to the saccharomycetes is 8:8:12:18; the concentration of the bacteria of the bacillus brevis, the lactobacillus, the bacillus megaterium and the saccharomycetes is independently 2 hundred million/ml;
the mass ratio of the bacillus thuringiensis to the bacillus licheniformis to the bacillus subtilis to the trichoderma viride to the aspergillus niger to the streptococcus thermophilus to the actinomycetes is 12:20:13:8:14:4:3; the bacterial concentration of the bacillus thuringiensis, the bacillus licheniformis, the bacillus subtilis, the trichoderma viride, the aspergillus niger, the streptococcus thermophilus and the actinomycetes is independently 1 hundred million/ml;
the mass ratio of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria is 22:13:11:4; the concentration of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria is independently 2 hundred million/ml;
the preparation method of the microbial agent comprises the following steps:
(1) Mixing Brevibacterium, lactobacillus, bacillus megatherium and saccharomycetes to obtain a solid fermentation microbial inoculum;
(2) Mixing bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomycetes to obtain a liquid fermentation microbial inoculum;
(3) Mixing chlorella, blue algae, candida tropicalis and photosynthetic bacteria to obtain the ammonia nitrogen remover.
Example 2
The microbial agent for treating the cow breeding wastewater is prepared from the following components in parts by weight: 18 parts of solid fermentation bacteria, 20 parts of liquid fermentation bacteria and 20 parts of ammonia nitrogen remover;
the solid fermentation inoculant is bacillus brevis, lactobacillus, bacillus megatherium and saccharomycetes;
the liquid fermentation inoculant is bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomyces;
the ammonia nitrogen remover is chlorella, blue algae, candida tropicalis and photosynthetic bacteria;
the mass ratio of the bacillus brevis to the lactobacillus to the bacillus megaterium to the saccharomycetes is 12:12:18:26; the concentration of the bacteria of the bacillus brevis, the lactobacillus, the bacillus megaterium and the saccharomycetes is independently 6 hundred million/ml;
the mass ratio of the bacillus thuringiensis to the bacillus licheniformis to the bacillus subtilis to the trichoderma viride to the aspergillus niger to the streptococcus thermophilus to the actinomycetes is 16:24:19:12:18:8:9; the bacterial concentration of the bacillus thuringiensis, the bacillus licheniformis, the bacillus subtilis, the trichoderma viride, the aspergillus niger, the streptococcus thermophilus and the actinomycetes is independently 4 hundred million/ml;
the mass ratio of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria is 28:19:17:12; the bacterial concentration of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria is independently 6 hundred million/ml;
the preparation method of the microbial agent comprises the following steps:
(1) Mixing Brevibacterium, lactobacillus, bacillus megatherium and saccharomycetes to obtain a solid fermentation microbial inoculum;
(2) Mixing bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomycetes to obtain a liquid fermentation microbial inoculum;
(3) Mixing chlorella, blue algae, candida tropicalis and photosynthetic bacteria to obtain the ammonia nitrogen remover.
Example 3
The microbial agent for treating the cow breeding wastewater is prepared from the following components in parts by weight: 15 parts of solid fermentation bacteria agent, 15 parts of liquid fermentation bacteria agent and 15 parts of ammonia nitrogen remover;
the solid fermentation inoculant is bacillus brevis, lactobacillus, bacillus megatherium and saccharomycetes;
the liquid fermentation inoculant is bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomyces;
the ammonia nitrogen remover is chlorella, blue algae, candida tropicalis and photosynthetic bacteria;
the mass ratio of the bacillus brevis to the lactobacillus to the bacillus megaterium to the saccharomycetes is 10:10:15:22; the concentration of the bacteria of the bacillus brevis, the lactobacillus, the bacillus megaterium and the saccharomycetes is independently 4 hundred million/ml;
the mass ratio of the bacillus thuringiensis to the bacillus licheniformis to the bacillus subtilis to the trichoderma viride to the aspergillus niger to the streptococcus thermophilus to the actinomycetes is 14:22:16:10:16:6:6; the bacterial concentration of the bacillus thuringiensis, the bacillus licheniformis, the bacillus subtilis, the trichoderma viride, the aspergillus niger, the streptococcus thermophilus and the actinomycetes is independently 2.5 hundred million/ml;
the mass ratio of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria is 25:16:14:8; the bacterial concentration of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria is independently 4 hundred million/ml;
the preparation method of the microbial agent comprises the following steps:
(1) Mixing Brevibacterium, lactobacillus, bacillus megatherium and saccharomycetes to obtain a solid fermentation microbial inoculum;
(2) Mixing bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomycetes to obtain a liquid fermentation microbial inoculum;
(3) Mixing chlorella, blue algae, candida tropicalis and photosynthetic bacteria to obtain the ammonia nitrogen remover.
Example 4
The preparation method of the organic fertilizer comprises the following steps:
(S1) performing solid-liquid separation on cow breeding wastewater to obtain filter residues and filtrate;
(S2) mixing the filter residue obtained in the step (S1) with the solid fermentation microbial inoculum described in the example 1, and carrying out normal-temperature solid fermentation for 7d to obtain a solid organic fertilizer; the mass ratio of the filter residue to the solid fermentation inoculant is 80:6, preparing a base material;
(S3) mixing the filtrate obtained in the step (S1) with the liquid fermentation microbial inoculum described in the example 1, and fermenting for 4d at 32 ℃ to obtain a fermentation liquor; the mass ratio of the filtrate to the liquid fermentation microbial inoculum is 80:4, a step of;
(S4) mixing the fermentation liquor obtained in the step (S3) with the ammonia nitrogen remover of the embodiment 1, and fermenting at 24 ℃ for 25 hours to obtain a liquid organic fertilizer; the mass ratio of the fermentation liquor to the ammonia nitrogen remover is 80:2.
example 5
The preparation method of the organic fertilizer comprises the following steps:
(S1) performing solid-liquid separation on cow breeding wastewater to obtain filter residues and filtrate;
(S2) mixing the filter residue obtained in the step (S1) with the solid fermentation microbial inoculum described in the example 2, and carrying out normal-temperature solid fermentation for 15d to obtain a solid organic fertilizer; the mass ratio of the filter residue to the solid fermentation inoculant is 100:10;
(S3) mixing the filtrate obtained in the step (S1) with the liquid fermentation microbial inoculum described in the example 2, and fermenting at 36 ℃ for 8d to obtain fermentation liquor; the mass ratio of the filtrate to the liquid fermentation microbial inoculum is 100:8, 8;
(S4) mixing the fermentation liquor obtained in the step (S3) with the ammonia nitrogen remover in the embodiment 2, and fermenting for 35 hours at the temperature of 28 ℃ to obtain a liquid organic fertilizer; the mass ratio of the fermentation liquor to the ammonia nitrogen remover is 100:6.
example 6
The preparation method of the organic fertilizer comprises the following steps:
(S1) performing solid-liquid separation on cow breeding wastewater to obtain filter residues and filtrate;
(S2) mixing the filter residue obtained in the step (S1) with the solid fermentation microbial inoculum described in the example 3, and carrying out normal-temperature solid fermentation for 11d to obtain a solid organic fertilizer; the mass ratio of the filter residue to the solid fermentation inoculant is 90:8, 8;
(S3) mixing the filtrate obtained in the step (S1) with the liquid fermentation microbial inoculum described in the example 3, and fermenting at 34 ℃ for 6d to obtain a fermentation liquor; the mass ratio of the filtrate to the liquid fermentation microbial inoculum is 90:6, preparing a base material;
(S4) mixing the fermentation liquor obtained in the step (S3) with the ammonia nitrogen remover in the embodiment 3, and fermenting for 30 hours at the temperature of 26 ℃ to obtain a liquid organic fertilizer; the mass ratio of the fermentation liquor to the ammonia nitrogen remover is 90:4.
example 7
Comparison test:
the waste water of the dairy farm was collected, subjected to solid-liquid separation, and the COD, ammonia nitrogen, kjeldahl nitrogen and total phosphorus content in the filtrate obtained by the separation were measured, and the results are shown in Table 1.
The measuring method comprises the following steps: the Chemical Oxygen Demand (COD) of the filtrate is measured by using a GB/11914-89 potassium dichromate method, the Total Phosphorus (TP) is measured by using a GB11893-89 potassium dichromate method, the KN is measured by using a CB11891-89 potassium dichromate method, and the ammonia nitrogen is measured by using a titration method.
TABLE 1 determination of the filtrate
Measurement index content/mg.L -1
COD 15684.3
Ammonia nitrogen 5136.8
Kjeldahl nitrogen 6831.3
Total phosphorus 14.2
The liquid organic fertilizer obtained by the method of example 6 was used as an experimental group;
control group 1: the procedure of example 6 was followed with the following modifications: after the fermentation in the step (3) is completed, adding a conventional ammonia nitrogen remover (purchased from a middle shellfish environment-friendly ZB-NH series ammonia nitrogen remover, the model is ZB-G02, the treatment is carried out according to a description method, and the adding proportion is the same as the using amount of the ammonia nitrogen remover in the embodiment 6) to treat the fermentation broth, thereby obtaining the liquid organic fertilizer.
Blank control group: the procedure of example 6 was followed with the following modifications: replacing the ammonia nitrogen remover with water with the same quality to obtain a liquid organic fertilizer;
except for different use of ammonia nitrogen remover, other methods of each test group are identical, each test group is repeatedly tested for 3 times, after the test is completed, the COD, ammonia nitrogen, kjeldahl nitrogen and total phosphorus content of the obtained liquid organic fertilizer of each treatment group are measured, and an average value is obtained. The results are shown in Table 2.
TABLE 2 liquid organic fertilizer measurement results
As can be seen from the contents recorded in table 2, the ammonia nitrogen remover provided by the invention has an excellent ammonia nitrogen removal effect, and has a better use effect compared with the common ammonia nitrogen remover of the control group 1.
Example 8
Selecting an agricultural land with the size of 1200m 2 Wheat was planted and divided into 12 areas on average, three areas were planted ad libitum for each test group.
The organic fertilizer obtained by the method of example 6 is used as an experimental group, wherein the liquid organic fertilizer is used by the following method: in the three-leaf period and the jointing period of the wheat, diluting the liquid organic fertilizer for 5 times, then flushing with water, and applying 20-30 kg of the diluted liquid organic fertilizer per mu each time;
the solid organic fertilizer is used as a base fertilizer before wheat planting, and 50-70 kg of the solid organic fertilizer is applied per mu.
Control group 2: the special fertilizer for wheat (special fertilizer for double-core wheat 18-15-12, purchased from Henan agricultural fertilizer industry Co., ltd.) is applied according to the method of the specification.
The application methods of the solid organic fertilizer and the liquid organic fertilizer obtained in the control group 1 and the blank control group are the same as those of the experimental group.
The test groups all have the same management method of other wheat except for different fertilizer use. After wheat is harvested, the acre yield is calculated and averaged. The results are shown in Table 3.
TABLE 3 mu yield
Yield/(kg/mu)
Experimental group 352.1
Control group 1 316.5
Control group 2 301.7
Blank control group 277.3
As shown in the results recorded in Table 3, the use effect of the organic fertilizer is better than that of the common fertilizer in the prior art, and the wheat yield of the experimental group is improved by 16.4% compared with that of the control group 2. The data of the control group 1 and the blank control group show that the ammonia nitrogen content in the liquid organic fertilizer can be influenced without using an ammonia nitrogen remover or using a conventional ammonia nitrogen remover, and more importantly, the high ammonia nitrogen in the organic fertilizer can influence the growth of crops, so that the yield of wheat is influenced. And the optimal use effect can be achieved only by selecting the ammonia nitrogen remover.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (10)

1. The microbial agent for treating the cow breeding wastewater is characterized by being prepared from the following components in parts by weight: 12-18 parts of solid fermentation microbial inoculum, 10-20 parts of liquid fermentation microbial inoculum and 10-20 parts of ammonia nitrogen remover;
the solid fermentation inoculant is bacillus brevis, lactobacillus, bacillus megatherium and saccharomycetes;
the liquid fermentation inoculant is bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomyces;
the ammonia nitrogen remover is chlorella, blue algae, candida tropicalis and photosynthetic bacteria;
the mass ratio of the bacillus brevis, the lactobacillus, the bacillus megatherium and the saccharomycetes is 8-12:8-12:12-18:18-26;
the mass ratio of the bacillus thuringiensis to the bacillus licheniformis to the bacillus subtilis to the trichoderma viride to the aspergillus niger to the streptococcus thermophilus to the actinomycetes is 12-16:20-24:13-19:8-12:14-18:4-8:3-9;
the mass ratio of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria is 22-28:13-19:11-17:4-12.
2. A microbial agent for treating cow breeding wastewater according to claim 1, wherein,
the concentration of the bacillus brevis, the lactobacillus, the bacillus megatherium and the saccharomycetes in the solid fermentation inoculant is independently 2-6 hundred million/ml;
the concentration of bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomycetes in the liquid fermentation inoculant is independently 1-4 hundred million/ml;
the concentration of the chlorella, the blue algae, the candida tropicalis and the photosynthetic bacteria in the ammonia nitrogen remover is independently 2-6 hundred million/ml.
3. The microbial agent for treating cow breeding wastewater according to claim 2, wherein the preparation method of the microbial agent comprises the following steps:
(1) Mixing Brevibacterium, lactobacillus, bacillus megatherium and saccharomycetes to obtain a solid fermentation microbial inoculum;
(2) Mixing bacillus thuringiensis, bacillus licheniformis, bacillus subtilis, trichoderma viride, aspergillus niger, streptococcus thermophilus and actinomycetes to obtain a liquid fermentation microbial inoculum;
(3) Mixing chlorella, blue algae, candida tropicalis and photosynthetic bacteria to obtain the ammonia nitrogen remover.
4. The preparation method of the organic fertilizer is characterized by comprising the following steps:
(S1) performing solid-liquid separation on cow breeding wastewater to obtain filter residues and filtrate;
(S2) mixing the filter residue obtained in the step (S1) with the solid fermentation microbial inoculum of claim 1 for solid fermentation to obtain a solid organic fertilizer;
(S3) mixing the filtrate obtained in the step (S1) with the liquid fermentation microbial inoculum of claim 1 for primary fermentation to obtain fermentation liquor;
and (S4) mixing the fermentation liquor obtained in the step (S3) with the ammonia nitrogen remover of claim 1 for secondary fermentation to obtain the liquid organic fertilizer.
5. The preparation method of the organic fertilizer according to claim 4, wherein the mass ratio of the filter residue in the step (S2) to the solid fermentation microbial inoculum in claim 1 is 80-100: 6 to 10.
6. The method for producing an organic fertilizer according to claim 4 or 5, wherein the solid fermentation in step (S2) is performed for 7 to 15 days.
7. The method for producing an organic fertilizer according to claim 4, wherein the temperature of the primary fermentation in the step (S3) is 32 to 36 ℃; the time of the primary fermentation is 4-8 d.
8. The method for preparing an organic fertilizer according to claim 4, wherein the secondary fermentation in the step (S4) is performed at a temperature of 24-28 ℃; the secondary fermentation time is 25-35 h.
9. The method for preparing the organic fertilizer according to claim 4 or 8, wherein the mass ratio of the filtrate 2 in the step (S3) to the liquid fermentation inoculant in claim 1 is 80-100: 4 to 8.
10. The method for preparing an organic fertilizer according to claim 4, wherein the mass ratio of the fermentation liquid in the step (S4) to the ammonia nitrogen remover in claim 1 is 80-100: 2 to 6.
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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103451129A (en) * 2013-07-16 2013-12-18 承德市畜牧研究所 Fermentation bacteria agent and fermentation method of excrements of livestock and poultry
RU2015100862A (en) * 2015-01-12 2016-08-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Курская государственная сельскохозяйственная академия имени профессора И.И. Иванова" Министерства сельского хозяйства Российской Федерации The method of disposal of livestock complexes
CN108793587A (en) * 2018-05-30 2018-11-13 北京六凯农业科技有限公司 A kind of immediate processing method of pig farm urine bubble excrement waste liquid
CN109020723A (en) * 2018-10-09 2018-12-18 江门市澳保生物科技有限公司 A kind of technique producing active phosphorus organic fertilizer used for aquiculture using Compo fermentation machine processing pig manure
CN111620724A (en) * 2020-05-28 2020-09-04 四川汇邦环保科技有限公司 Pig manure organic fertilizer fermentation inoculant and application method thereof
CN112299562A (en) * 2019-07-29 2021-02-02 仲恺农业工程学院 Method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion
CN114410507A (en) * 2021-12-20 2022-04-29 南京市畜牧家禽科学研究所(南京市畜牧技术推广站) Development process and application of microorganisms in animal excrement of farm

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103451129A (en) * 2013-07-16 2013-12-18 承德市畜牧研究所 Fermentation bacteria agent and fermentation method of excrements of livestock and poultry
RU2015100862A (en) * 2015-01-12 2016-08-10 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Курская государственная сельскохозяйственная академия имени профессора И.И. Иванова" Министерства сельского хозяйства Российской Федерации The method of disposal of livestock complexes
CN108793587A (en) * 2018-05-30 2018-11-13 北京六凯农业科技有限公司 A kind of immediate processing method of pig farm urine bubble excrement waste liquid
CN109020723A (en) * 2018-10-09 2018-12-18 江门市澳保生物科技有限公司 A kind of technique producing active phosphorus organic fertilizer used for aquiculture using Compo fermentation machine processing pig manure
CN112299562A (en) * 2019-07-29 2021-02-02 仲恺农业工程学院 Method for promoting microalgae to degrade carbon, nitrogen and phosphorus by using yeast secretion
CN111620724A (en) * 2020-05-28 2020-09-04 四川汇邦环保科技有限公司 Pig manure organic fertilizer fermentation inoculant and application method thereof
CN114410507A (en) * 2021-12-20 2022-04-29 南京市畜牧家禽科学研究所(南京市畜牧技术推广站) Development process and application of microorganisms in animal excrement of farm

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Zepeng Zhang 等."Full-scale thermophilic aerobic co-composting of blue-green algae sludge with livestock faeces and straw".《Sci Total Environ.》.2020,第753卷第1-10页. *
侯万文 等.《简明科学养猪手册》.金盾出版社,2003,(第1版),第149页. *
希利 舒瓦尔.《水的再净与再用》.中国建筑工业出版社,1986,(第1版),第234页. *
王清印.《海水设施养殖》.海洋出版社,2004,(第1版),第289页. *
赵秋菊 等."生物接触氧化工艺处理奶牛养殖废水参数优化研究".《水处理技术》.2015,第41卷(第8期),第115-120页. *

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