CN117101741A - Molecular detection assembly line storehouse body structure and molecular detection assembly line - Google Patents
Molecular detection assembly line storehouse body structure and molecular detection assembly line Download PDFInfo
- Publication number
- CN117101741A CN117101741A CN202311361047.6A CN202311361047A CN117101741A CN 117101741 A CN117101741 A CN 117101741A CN 202311361047 A CN202311361047 A CN 202311361047A CN 117101741 A CN117101741 A CN 117101741A
- Authority
- CN
- China
- Prior art keywords
- space
- window
- air
- sample
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 55
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 106
- 238000002360 preparation method Methods 0.000 claims abstract description 102
- 230000003321 amplification Effects 0.000 claims abstract description 71
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 71
- 238000000034 method Methods 0.000 claims abstract description 14
- 230000007246 mechanism Effects 0.000 claims description 13
- 238000002955 isolation Methods 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 238000000429 assembly Methods 0.000 claims description 9
- 230000000712 assembly Effects 0.000 claims description 9
- 238000012546 transfer Methods 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 4
- 238000007836 assay cartridge Methods 0.000 claims 2
- 230000008569 process Effects 0.000 abstract description 7
- 239000003570 air Substances 0.000 description 202
- 239000007789 gas Substances 0.000 description 29
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 9
- 238000007789 sealing Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 238000012408 PCR amplification Methods 0.000 description 5
- 238000012864 cross contamination Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 230000004544 DNA amplification Effects 0.000 description 2
- 230000003749 cleanliness Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 206010063385 Intellectualisation Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009510 drug design Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003722 extracellular fluid Anatomy 0.000 description 1
- 239000000295 fuel oil Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
- B01L1/02—Air-pressure chambers; Air-locks therefor
- B01L1/025—Environmental chambers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/143—Quality control, feedback systems
- B01L2200/146—Employing pressure sensors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/14—Means for pressure control
Landscapes
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The application discloses a molecular detection assembly line bin body structure and a molecular detection assembly line, and relates to the technical field of molecular detection, wherein the molecular detection assembly line bin body structure comprises a reagent preparation bin, a sample preparation bin and a product amplification bin, and the reagent preparation bin is provided with a reagent preparation space for preparing reagents; the sample preparation bin has a sample preparation space for extracting a sample and preparing a sample to be amplified; the product amplification bin is provided with a product amplification space for amplifying and detecting the sample; the air pressure in the reagent preparation space is larger than the air pressure in the sample preparation space and the air pressure in the product amplification space, and the air pressure in the sample preparation space and the air pressure in the product amplification space are smaller than the external air pressure, so that the air in the sample preparation space and the air pressure in the product amplification space can be restrained from flowing into the reagent preparation space, the pollution to the reagent is reduced, the cross pollution in the molecular detection process is further reduced, and the detection accuracy is improved.
Description
Technical Field
The application relates to molecular detection equipment, in particular to a molecular detection assembly line bin body structure and a molecular detection assembly line.
Background
Molecular diagnosis refers to a technique of detecting changes in the structure or expression level of genetic material using molecular biological methods to make a diagnosis. For example, nucleic acid detection is a molecular detection.
During the new crown epidemic situation, large-scale nucleic acid detection becomes a new need, and the market for basic nucleic acid detection is promoted. However, the traditional molecular detection flow has complicated steps, and has the defects of high labor cost, high laboratory maintenance cost, long time consumption, high requirements on places, environments and operators, difficulty in ensuring the safety and stability of experiments and the like.
Therefore, integration, automation and intellectualization of molecular detection are also demands of industry, and the intelligent industrial robot molecular diagnosis pipeline tends to replace the existing traditional molecular detection.
Molecular diagnostics typically include steps such as reagent preparation (e.g., preparing amplification reagents), sample preparation (e.g., extracting a sample, adding reagents, membrane-sealing centrifugation), and product amplification (amplifying a sample prepared by the preceding steps with a PCR amplification apparatus). The inventor researches and discovers that with the development of automation technology, it is possible to design a molecular detection pipeline capable of automatically completing the above steps of molecular diagnosis, for example, a full-automatic nucleic acid sample processing system disclosed in chinese patent application publication No. CN116396850a, which can realize automatic detection of nucleic acid samples. However, during the molecular detection process, some toxic or infectious liquid (such as a nucleic acid sample and an extracted or amplified product thereof) may exist, and after the liquid volatilizes into the air, the liquid flows between the bin bodies of each molecular detection pipeline, and may cause cross contamination to reagents or samples in each bin body.
In addition, if the internal gas in storehouse overflows to the assembly line outside, is inhaled by external personnel, will cause adverse effect to external personnel health, if external gas gets into the internal portion in storehouse, still probably can cause the pollution to internal test, consequently, need the storehouse body structure of rational design molecular detection line to improve the security, guarantee the accuracy of detection.
Disclosure of Invention
The application aims to provide a molecular detection assembly line bin body structure and a molecular detection assembly line, which can effectively reduce cross contamination in the molecular detection process.
In order to achieve the above object, the present application provides a molecular detection assembly line bin structure, comprising:
a reagent preparing bin having a reagent preparing space for preparing a reagent;
a sample preparation bin having a sample preparation space for extracting a sample and preparing a sample to be amplified; the method comprises the steps of,
a product amplification chamber having a product amplification space for amplifying and detecting the sample;
the air pressure in the reagent preparation space is greater than the air pressure in the sample preparation space and the product amplification space, and the air pressure in both the sample preparation space and the product amplification space is less than the external air pressure.
Further, the gas pressure within the sample preparation space is greater than the gas pressure within the product amplification space.
Further, the air pressure in the reagent preparing space is equal to or higher than the external air pressure.
Further, the difference between the air pressure in the reagent preparation space and the external air pressure is 0-15 pa, the difference between the sample preparation space and the external air pressure is-15 pa to-5 pa, and the difference between the air pressure in the product amplification space and the air pressure in the sample preparation space is-15 pa to-5 pa.
Further, the reagent preparation bin comprises a first air flow conveying device for supplying air to the reagent preparation space;
the sample preparation bin comprises a second airflow conveying device for exhausting the sample preparation space;
the product amplification bin comprises a third air flow conveying device for exhausting the product amplification space;
the reagent preparation bin, the sample preparation bin and the product amplification bin each comprise an air pressure sensor for detecting an air pressure value in a corresponding space.
Further, the reagent preparing bin comprises a first air inlet and a first air outlet which are communicated with the reagent preparing space, the first air flow conveying device is communicated with the first air inlet, one of the first air inlet and the first air outlet is arranged above the reagent preparing space, and the other is arranged below the reagent preparing space;
the sample preparation bin comprises a second air inlet and a second air outlet which are communicated with the sample preparation space, the second air flow conveying device is communicated with the second air outlet, one of the second air inlet and the second air outlet is arranged above the sample preparation space, and the other of the second air inlet and the second air outlet is arranged below the sample preparation space;
the product amplification bin comprises a third air inlet and a third air outlet which are communicated with the product amplification space, the third air flow conveying device is communicated with the third air outlet, one of the third air inlet and the third air outlet is arranged above the product amplification space, and the other of the third air inlet and the third air outlet is arranged below the product amplification space.
Further, the first air inlet, the first air outlet, the second air inlet, the second air outlet, the third air inlet and the third air outlet are all provided with air purifying devices for filtering gas;
and sterilizing devices for sterilizing the interior are arranged in the reagent preparation space, the sample preparation space and the product amplification space, and the sterilizing devices are ultraviolet lamps. Further, an isolation conveyor is provided between the reagent preparation cartridge and the sample preparation cartridge and/or between the sample preparation cartridge and the product amplification cartridge, the isolation conveyor comprising:
the shell is provided with a conveying space for accommodating objects to be conveyed, and a first window and a second window which are communicated with the conveying space, wherein the first window and the second window are respectively positioned in two bins;
the first window body is rotatably connected with the shell and is used for sealing the first window;
the second window body is rotatably connected with the shell and is used for sealing the second window; the method comprises the steps of,
and the opening and closing mechanism is used for driving the first window body and the second window body to be opened and closed, and when one of the first window body and the second window body is in an opened state, the other is in a closed state.
Further, the opening and closing mechanism comprises a linear driver, a supporting plate which is driven by the linear driver to do linear motion, a rack connected with the supporting plate and two groups of windowing components, wherein the windowing components comprise a gear rotatably connected with the shell and a connecting rod connected with the gear, and the gear is positioned on a moving path of the rack and is used for being meshed with the rack;
one connecting rod of the two groups of window opening assemblies is used for lifting the first window under the drive of the gear, and the other connecting rod is used for lifting the second window under the drive of the gear.
Further, when the first window and the second window are both in a closed state, the gears of the two groups of window opening assemblies are respectively positioned at two ends of the rack, and the rack is meshed with only one group of gears of the window opening assemblies at a time.
Further, the isolation conveying device further comprises two limiting pieces which are fixed relative to the shell, the two limiting pieces are located below the first window body and the second window body respectively, and the limiting pieces are used for limiting the lower limit position of the connecting rod.
On the other hand, the application provides a molecular detection assembly line, which comprises the molecular detection assembly line bin body structure.
Compared with the prior art, the application has the following beneficial effects:
according to at least one embodiment of the application, the molecular detection assembly line bin body structure comprises a reagent preparation bin, a sample preparation bin and a product amplification bin, the air pressure in the reagent preparation space is larger than the air pressure in the sample preparation space and the air pressure in the product amplification space, and the air pressure in the sample preparation space and the air pressure in the product amplification space are smaller than the external air pressure, so that the air in the sample preparation space and the air pressure in the product amplification space can be restrained from flowing into the reagent preparation space, the pollution to the reagent is reduced, the cross pollution in the molecular detection process is further reduced, and the detection accuracy is improved.
Drawings
FIG. 1 is a front view of a molecular pipeline cartridge structure according to one embodiment of the present application.
Fig. 2 is a perspective view of the molecular pipeline cartridge structure shown in fig. 1.
FIG. 3 is a schematic view of the molecular pipeline cartridge body structure shown in FIG. 2 with a portion of the closure plate removed.
FIG. 4 is a perspective view of a reagent dispensing cartridge according to one embodiment of the present application.
Fig. 5 is a perspective view of a sample preparation cartridge according to one embodiment of the present application.
FIG. 6 is a perspective view of a product amplification cartridge according to one embodiment of the present application.
FIG. 7 is a schematic illustration of the position of a sample preparation cartridge and isolation conveyor of one embodiment of the application.
Fig. 8 is a perspective view of an isolated conveyance device according to one embodiment of the present application.
Fig. 9 is a schematic view of the isolation conveyor of fig. 8 without a window.
Fig. 10 is a cross-sectional view of an isolated conveyance device according to one embodiment of the present application.
Fig. 11 is an internal structural view of an isolated transport apparatus according to an embodiment of the present application.
Detailed Description
In order that the above objects, features and advantages of the application will be readily understood, a more particular description of the application will be rendered by reference to the appended drawings. It is to be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the application. It should be further noted that, for convenience of description, only some, but not all of the structures related to the present application are shown in the drawings. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The terms "comprising" and "having" and any variations thereof herein are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed steps or elements but may include other steps or elements not listed or inherent to such process, method, article, or apparatus.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
As shown in fig. 1 and 2, a molecular detection pipeline cartridge body structure corresponding to a preferred embodiment of the present application includes a reagent preparing cartridge 1, a sample preparing cartridge 2 and a product amplifying cartridge 3, the demarcation lines of each cartridge being shown in dotted lines.
The reagent preparing chamber 1 is used for preparing a reagent which is required to be used in the subsequent molecular detection process, for example, an amplification reagent which is required to be used in the PCR amplification, and the kind of the reagent which is prepared in the reagent preparing chamber 1 may be one or more kinds. The structure for preparing the reagent is not limited, for example, an industrial robot, a liquid transfer pump connected with the industrial robot and a disposable liquid transfer suction head can be arranged, the industrial robot sucks the reagent in the reagent bottle through the liquid transfer pump and the liquid transfer suction head to disperse the reagent into a micro-pore plate or other containers, and various solutions can be mixed according to a proportion for preparation. The reagent in the reagent preparing bin 1 is conveyed to a subsequent corresponding bin body for use after the preparation is completed, and the conveying of the microplate can be realized by means of assembly line or industrial robot conveying and the like.
As shown in fig. 3 and 4, the reagent preparing chamber 1 has a reagent preparing space 10 for preparing a reagent, and the reagent preparing space 10 may be formed by enclosing a frame with a sealing plate, and the reagent preparing operation is performed in the reagent preparing space 10.
The sample preparation cartridge 2 is used for extracting a sample and preparing a sample to be amplified. The sample is, for example, interstitial fluid, blood, saliva or tissue obtained by collection, etc., which is typically provided in a sampling tube, and the items to be tested for the sample include, but are not limited to, neo-crown, HPV, ground-deficit, tuberculosis, HIV, hepatitis b. In the sample preparation magazine 2, an industrial robot can be arranged to cooperate with an electrically driven rotary jaw to open the lid of the sampling tube, after which the sample in the sampling tube is sucked out by means of suction and then added to a corresponding deep-well plate or other container, where the position of the sample in the deep-well plate can be recorded. A nucleic acid extractor, a membrane sealing device and centrifugal equipment are arranged in the sample preparation bin 2. After the sample is sucked into the deep hole plate, the sample can be conveyed into a nucleic acid extraction instrument by an industrial robot or other modes, the sample nucleic acid is extracted, after the extraction is finished, the sample is added into a micro hole plate conveyed from a reagent preparation bin 1 by a liquid transfer pump in a sucking mode, the sample is mixed with an amplification reagent, a sample to be amplified is obtained, then the micro hole plate is sealed by a sealing film device, and then the sealed micro hole plate is placed into a centrifugal device for centrifugation, so that the sample and the amplification reagent are fully mixed.
As shown in fig. 3 and 5, the sample preparation chamber 2 has a sample preparation space 20 for extracting a sample and preparing a sample to be amplified, and the sample preparation space 20 may be enclosed in the form of a rack-and-close plate, and the operation of extracting a sample and preparing a sample to be amplified is performed in the reagent preparation space 20.
After centrifugation, the sample to be amplified in the microplate is sent to the product amplification bin 3, and the structure of the microplate can be transported by a belt, an industrial robot or other modes.
The product amplification bin 3 is used for amplifying and detecting a sample, and specifically comprises a PCR amplification instrument and an industrial robot for carrying a microplate, wherein the industrial robot can carry the microplate in a clamping mode through clamping jaws, for example, the microplate can be moved into the PCR amplification instrument for DNA amplification, the PCR amplification instrument can automatically detect and output a detection result, and the amplified microplate is taken out by the industrial robot and put into a collection box.
As shown in fig. 3 and 6, the product amplification bin 3 has a product amplification space 30 for amplifying and detecting a sample to be amplified, and the product amplification space 30 can also be formed by enclosing a frame with a sealing plate, and the operation of amplifying and detecting the sample to be amplified is performed in the product amplification space 30.
The air pressure in the reagent preparing space 10 is set to be larger than the air pressure in the sample preparing space 20 and the product amplifying space 30, and the air pressure in the sample preparing space 20 and the product amplifying space 30 is smaller than the air pressure in the outside, that is, the inside of the sample preparing space 20 and the inside of the product amplifying space 30 are in a negative pressure state. The external air pressure refers to the ambient air pressure at which the molecular detection line is placed, and the molecular detection line is typically placed in an atmospheric environment, and thus the external air pressure is typically equal to the standard atmospheric pressure.
The sample may be contained in the gas in the inner space due to the exposure of the sample in the sample preparation space 20 and the product amplification space 30, and the concentration of the sample in the gas may be higher due to the gene amplification in the product amplification space 30, and there is a certain risk of infection due to the outdiffusion of these gases. Therefore, the sample preparation space 20 and the product amplification space 30 are provided in the form of negative pressure, which is advantageous in reducing the risk of gas overflowing from the slit of the cartridge body to the outside of the cartridge body, thereby improving safety.
Further, the air pressure in the reagent preparing space 10 is set to be larger than the air pressure in the sample preparing space 20 and the product amplifying space 30, so that the air pressure in the sample preparing space 20 and the product amplifying space 30 can be prevented from entering the reagent preparing space 10 to pollute the reagent in the reagent preparing space 10, and thus, the accuracy of the detection result can be improved.
In some embodiments, the air pressure within the sample preparation space 20 is set to be greater than the air pressure within the product amplification space 30. The air pressure in the product amplification space 30 is set to be smaller than the air pressure in the sample preparation space 20, so that gradient negative pressure among the reagent preparation space 10, the sample preparation space 20 and the product amplification space 30 is realized, the flow of air is consistent with the moving direction of the reagent, the sample and the like, the air in the product amplification space 30 is prevented from entering the sample preparation space 20 and polluting the sample in the sample preparation space 20, cross contamination of internal air is further prevented, and the accuracy of a detection result is improved.
In some embodiments, the air pressure within the reagent dispensing space 10 is set to be equal to or greater than the external air pressure. Since the reagent preparing operation in the reagent preparing space 10 does not generally generate harmful or polluting gas, the air pressure in the reagent preparing space 10 is set to a positive pressure, so that the air sucked into the outside can be prevented, and the air in the sample preparing chamber 2 can be further prevented from entering the reagent preparing space 10.
In a preferred embodiment, the difference between the air pressure in the reagent preparing space 10 and the external air pressure (i.e., the value obtained by subtracting the external air pressure from the air pressure in the reagent preparing space 10) is 0 to 15pa, for example, 0 to 5pa, 5 to 10pa, 10 to 15pa, etc., the difference between the air pressure in the sample preparing space 20 and the external air pressure (i.e., the value obtained by subtracting the external air pressure from the air pressure in the sample preparing space 20) is-15 pa to-5 pa, for example, -15pa to-10 pa, -10pa to-5 pa, etc., and the difference between the air pressure in the product amplifying space 30 and the air pressure in the sample preparing space 20 (i.e., the value obtained by subtracting the air pressure in the sample preparing space 20) is-15 pa to-5 pa, for example, -15pa to-10 pa to-5 pa, etc.
In some embodiments, the difference between the air pressure in the reagent preparing space 10 and the external air pressure is 10pa, the difference between the air pressure in the sample preparing space 20 and the external air pressure is-10 pa, and the difference between the air pressure in the product amplifying space 30 and the external air pressure is-20 pa.
The air pressure in each bin body can be regulated by adopting an air flow conveying device, wherein the air flow conveying device is a device for driving air to flow by taking electric power or fuel oil and the like as energy sources, and can be a centrifugal fan for example.
As shown in fig. 4, the reagent preparing chamber 1 includes a first air flow transporting device 11 for supplying air to the reagent preparing space 10, and the air pressure in the reagent preparing space 10 is increased by feeding air flow into the reagent preparing space 10 through the first air flow transporting device 11. The reagent preparing bin 1 comprises a first air inlet and a first air outlet which are communicated with the reagent preparing space 10, the first air flow conveying device 11 is communicated with the first air inlet, for example, the first air flow conveying device can be directly arranged at the first air inlet or connected with the first air inlet through a pipeline, and the air conveyed by the first air flow conveying device 11 enters the reagent preparing space 10 through the first air inlet and is discharged from the first air outlet.
The first air flow transporting means 11 is not limited in position and may be mounted, for example, on the top, side or bottom of the reagent preparation chamber 1. In the embodiment shown in fig. 4, the first air flow transporting means 11 is arranged on top of the reagent preparation chamber 1 above the reagent preparation space 10. The first air inlet and the first air outlet are not limited in position, and are preferably provided at both upper and lower ends of the reagent preparing chamber 1, for example, one of them may be provided above the reagent preparing space 10 and the other below the reagent preparing space 10 to reduce inhalation of the gas discharged from the first air outlet.
As shown in fig. 5, the sample preparation chamber 2 includes a second air flow transporting device 21 for evacuating the sample preparation space 20, and the air pressure in the sample preparation space 20 can be reduced by evacuating the sample preparation space 20 through the second air flow transporting device 21. The sample preparation chamber 2 comprises a second air inlet and a second air outlet which are communicated with the sample preparation space 20, and the second air flow conveying device 21 is communicated with the second air outlet, for example, the second air flow conveying device can be directly arranged at the second air outlet or is connected with the second air outlet through a pipeline. The second air flow conveyor 21 extracts air from the second air outlet, and external air flow is input into the sample preparation space 20 from the second air inlet.
The mounting position of the second air flow conveyor 21 is not limited and may be mounted, for example, on the top, side or bottom of the sample preparation chamber 2, in the embodiment shown in fig. 5, the second air flow conveyor 21 is arranged on top of the sample preparation chamber 2 above the sample preparation space 20. It will be appreciated that the second airflow delivery device 21 may extend through a duct to the second outlet to communicate with the second outlet.
The positions of the second air inlet and the second air outlet on the sample preparation bin 2 are not limited, and are preferably located at the upper side and the lower side, and further preferably, the second air outlet is arranged below the sample preparation space 20, so that the air exhausted from the second air outlet is closer to the ground and is not easy to be directly inhaled into a human body, even if a small amount of inactive virus or bacteria are contained, the infection is not easy to be caused, the second air inlet is located above the sample preparation space 20, can be far away from the second air outlet, can inhale cleaner air, and reduces the air exhausted from the second air outlet.
As shown in fig. 6, the product amplification chamber 3 includes a third air flow transport device 31 for evacuating the product amplification space 30, and the air pressure in the product amplification space 30 can be reduced by evacuating the product amplification space 30 through the third air flow transport device 31. The product amplification bin 3 comprises a third air inlet and a third air outlet which are communicated with the product amplification space 30, and the third air flow conveying device 31 is communicated with the third air outlet, for example, the product amplification bin can be directly arranged at the third air outlet or connected with the third air outlet through a pipeline. The third air flow conveyor 31 extracts air from the third air outlet, and the external air flow is input into the product amplification space 30 from the third air inlet.
The third air flow transporting means 31 is not limited in installation position and may be installed, for example, on the top, side or bottom of the product amplification chamber 3, and in the embodiment shown in fig. 6, the third air flow transporting means 31 is provided on the top of the product amplification chamber 3 above the product amplification space 30. It will be appreciated that the third air flow conveying means 31 may extend through a duct to the third air outlet to communicate with the third air outlet.
The third air inlet and the third air outlet are not limited in position on the product amplification bin 3, and are preferably located at the upper side and the lower side, and further preferably, the third air outlet is arranged below the product amplification space 30, so that the gas exhausted from the third air outlet is closer to the ground and is not easy to be directly inhaled into a human body, even if a small amount of inactive virus or bacteria are contained, the infection is not easy to be caused, the third air inlet is located above the product amplification space 30, can be far away from the third air outlet, can inhale cleaner air, and reduces the gas exhausted from the third air outlet.
When the second air outlet and the third air outlet are located below, the first air inlet of the reagent preparing bin 1 is preferably located above the reagent preparing space 10, so as to reduce the gas sucked into the second air outlet and the third air outlet and make the gas entering the reagent preparing space 10 cleaner.
As a preferred embodiment, the reagent preparing chamber 1, the sample preparing chamber 2 and the product amplifying chamber 3 each include an air pressure sensor (not shown) for detecting an air pressure value in the corresponding space, that is, the reagent preparing space 10, the sample preparing space 20 and the product amplifying space 30 are each provided with an air pressure sensor to detect an air pressure value. The air pressure sensor and each air flow conveying device are all in communication connection with a control system of the molecular detection assembly line, and the control system can control the air flow conveying device according to the air pressure value detected by the air pressure sensor, for example, control parameters such as start and stop of the air flow conveying device, wind speed and the like, so that closed-loop control is realized, and the air pressure in each bin body is more accurately maintained.
In some embodiments, in order to ensure the cleanliness of the gas input into the bin body and exhausted from the bin body, air purifying devices for filtering the gas are arranged at each air inlet and each air outlet (namely, the first air inlet, the first air outlet, the second air inlet, the second air outlet, the third air inlet and the third air outlet), and the pollution of foreign matters mixed in the external gas to the inside of the bin body can be prevented through the filtration of dust, particles and the like in the air by the air purifying devices. The air purifying device can also have the function of disinfection and sterilization at the same time, so as to further prevent the pollution of the external air to the inside of the bin body and improve the cleanliness of the air exhausted from the bin body.
In some embodiments, in order to enhance the safety of the gas discharged from each cartridge body, a sterilizing device for sterilizing the inside, which may be, for example, an ultraviolet lamp, is provided in each of the reagent preparing space 10, the sample preparing space 20, and the product amplifying space 30. The number of the sterilizing devices can be one or more, and the virus and bacteria content in the gas is reduced in a sterilizing mode, so that the gas in the bin body is safer, and the cross contamination in the bin body and the pollution of the discharged gas to the outside of the bin body can be further reduced.
The reagent preparing bin 1, the sample preparing bin 2 and the product amplifying bin 3 are connected in sequence, and as described above, objects need to be transferred between the reagent preparing bin 1 and the sample preparing bin 2 and between the sample preparing bin 2 and the product amplifying bin 3. In this way, in order to achieve the relative sealing of the respective chambers, it is necessary to provide a channel that can be opened and closed between the respective chambers, but if a conventional channel structure is adopted, when the channel is opened, the two chambers are communicated, so that gas exchange occurs, and the stability of the internal gas pressure of the respective chambers is affected.
To solve this problem, isolation conveyors 4 are provided between the reagent preparation cartridge 1 and the sample preparation cartridge 2 and/or between the sample preparation cartridge 2 and the product amplification cartridge 3.
As shown in fig. 4 to 7, a partition 5 is provided between two adjacent bin bodies, a through hole 50 is provided on the partition 5, the isolation conveyor 4 is installed in the through hole 50, and a seal is formed between the outer periphery thereof and the through hole 50.
As shown in fig. 8 to 11, the isolation conveyor 4 includes a housing 40, a first window 41, a second window 42, and an opening and closing mechanism.
The housing 40 is provided with a conveying space 400 accommodating the objects 6 to be conveyed (e.g., a microplate or a multi-well plate, etc.), and a first window 402 and a second window 403 each communicating with the conveying space 400. The housing 40 is positioned at each end in two compartments, as are the first and second windows 402, 403. Preferably, the end surfaces of the first window 402 and the second window 403 are obliquely disposed obliquely upward so as to facilitate the removal of the objects 6 to be conveyed from the inside of the housing 40. Because the two windows are respectively positioned in the two bins, the objects 6 to be conveyed can be put in from the window positioned in one bin, then the objects 6 to be conveyed are conveyed to the other window, and then the objects 6 to be conveyed are taken out from the window, so that the objects 6 to be conveyed are conveyed between the two bins.
The first window 41 is rotatably connected to the housing 40, and the rotatable connection may be, for example, a hinge connection, a shaft connection, or the like, and the first window 41 is connected to an upper end of the first window 402, so as to seal the first window 402. When first window 41 is rotated into engagement with first window 402, first window 402 is sealed, whereas when first window 41 is rotated out of engagement with first window 402, first window 402 is opened.
The second window 42 is rotatably connected to the housing 40, and the rotatable connection may be, for example, a hinge connection, a shaft connection, or the like, and in fig. 7, the first window 41 and the second window 42 are connected to the housing 40 by hinges 44. The second window 42 is connected to the upper end of the second window 403, and is used for sealing the second window 403. The second window 403 is sealed when the second window 42 is rotated into engagement with the second window 403, whereas the second window 403 is opened when the second window 42 is rotated into disengagement with the second window 403.
The opening and closing mechanism is used for driving the first window 41 and the second window 42 to open and close, and when any window is in an open state, the other window is in a closed state, namely, when the first window 41 is open, the second window 42 is kept closed, and when the second window 42 is open, the first window 41 is kept closed. Thus, the first window 41 and the second window 42 are not simultaneously opened (may be simultaneously closed), and the stability of the air pressure between the two chambers can be ensured.
The opening and closing mechanism may be provided with two sets of driving mechanisms to open and close the two windows respectively, for example, each window is provided with a cylinder or an electric cylinder as a driving mechanism, and opening and closing actions of the windows are realized by extension and contraction of a driving shaft of the cylinder or the electric cylinder.
The opening and closing mechanism may be provided with only one driving mechanism to open and close the two window bodies, and as shown in fig. 10 and 11, the opening and closing mechanism includes a linear driver 430, a pallet 431 driven by the linear driver 430 to perform linear movement, a rack 432 connected to the pallet 431, and two sets of window opening units 433.
The linear actuator 430 may be, for example, a cylinder, an electric cylinder, a belt conveyor, a servo slide table, or the like, which has a linear driving function. In the embodiment shown in the drawings, the linear driver 430 is a servo sliding table, the supporting plate 431 is connected with the sliding table of the linear driver 430, and is driven by the linear driver 430 to perform linear motion, and the supporting plate 431 is used for carrying the objects 6 to be conveyed, so as to drive the objects 6 to be conveyed to move. The first window 402 and the second window 403 are both located on the moving path of the object 6 to be conveyed, so that the object 6 to be conveyed can be conveyed to the positions of the first window 402 and the second window 403, and the object 6 to be conveyed can be conveniently put in and taken out.
The rack 432 is fixedly coupled to the pallet 431 and disposed along a moving direction of the pallet 431.
The windowing assembly 433 includes a gear 4330 rotatably coupled to the sidewall 40a of the housing 40 and a link 4331 coupled to the gear 4330, the gear 4330 being positioned on a moving path of the rack 432 for engagement with the rack 432. One of the two sets of links 4331 of the window opening assemblies 433 is used for lifting the first window 41 under the driving of the gear 4330, and the other link 4331 is used for lifting the second window 42 under the driving of the gear 4330.
Specifically, the two sets of window assemblies 433 are disposed corresponding to the first window 41 and the second window 42 (in the figure, disposed below the windows), and the two connecting rods 4331 extend toward two ends of the housing 40. The length of the toothed portion of the rack 432 is smaller than the center distance between the two gears 4330, when the first window 41 and the second window 42 are both in the closed state, the gears 4330 of the two sets of window opening assemblies 433 are respectively located at two ends of the rack 432, and the rack 432 is meshed with the gears 4330 of one set of window opening assemblies 433 only at a time, and is not meshed with the two gears 4330 at the same time. As shown in fig. 10, when the rack 432 moves toward the end of the second window 42, it will engage the gear 4330 of the window assembly 433 corresponding to the second window 42, thereby driving the corresponding link 4331 to rotate to lift the second window 42 upward. When the rack 432 moves toward the end of the first window 41, it is disengaged from the gear 4330 of the window opening assembly 433 corresponding to the second window 42, so that the second window 42 is switched to the closed state, and then is engaged with the gear 4330 of the window opening assembly 433 corresponding to the first window 41, so as to drive the gear 4330 and the corresponding connecting rod 4331 to rotate, and the connecting rod 4331 lifts the second window 42.
The above-described opening and closing mechanism can realize the alternate opening and closing of the first window 41 and the second window 42 by using one linear actuator 430, and has lower cost and more reliable function realization. Obviously, when the rack 432 is in the neutral position, disengaged from the two gears 4330, both windows will be in the closed position.
As a preferred embodiment, as shown in fig. 10, the isolation conveyor 4 further includes two stoppers 45 connected to the sidewall 40a of the housing 40, and the two stoppers 45 are respectively located under the first window 41 and the second window 42 for restricting the lower limit position of the link 4331. The connecting rod 4331 is located between the corresponding window and the limiting piece 45, when the gear 4330 is disengaged from the rack 432, the connecting rod 4331 is supported by the limiting piece 45, the position of the connecting rod is closer to the window, and when the rack 432 is engaged with the gear 4330, the connecting rod 4331 can swing more rapidly to be in contact with the corresponding window, so that the window opening operation is completed, and the stroke required by the rack 432 for opening the window can be shortened.
It will be appreciated that the rack 432 need not be configured to engage only one gear 4330 at a time, but may be configured to remain engaged with both gears 4330 for a certain period of time or at all times.
It will be appreciated that since the first window 41 and the second window 42 are closed when one is opened, the two chambers are not communicated by the delivery of the object 6 to be delivered, so that the gas exchange between the two chambers can be effectively reduced, the fluctuation of the gas pressure in the chambers can be reduced, and the gas tends to flow to the low pressure area due to the pressure difference between the adjacent two chambers, so that the gas in the product amplification space 30 can be effectively reduced to flow into the sample preparation space 20, and the gas in the sample preparation space 20 can be effectively reduced to flow into the reagent preparation space 10.
The application also provides a molecular detection assembly line, which comprises the molecular detection assembly line bin body structure.
The foregoing is merely exemplary of the application and other modifications can be made without departing from the scope of the application.
Claims (11)
1. Molecular detection assembly line storehouse body structure, its characterized in that includes:
a reagent preparing chamber (1) having a reagent preparing space (10) for preparing a reagent;
a sample preparation cartridge (2) having a sample preparation space (20) for extracting a sample and preparing a sample to be amplified; the method comprises the steps of,
a product amplification chamber (3) having a product amplification space (30) for amplifying and detecting the sample;
the air pressure in the reagent preparing space (10) is greater than the air pressure in the sample preparing space (20) and the product amplifying space (30), and the air pressure in the sample preparing space (20) and the product amplifying space (30) is less than the external air pressure,
the reagent preparation chamber (1) comprises a first air flow conveying device (11) for supplying air to the reagent preparation space (10);
the sample preparation cartridge (2) comprises a second air flow conveyor (21) for evacuating the sample preparation space (20);
the product amplification bin (3) comprises a third air flow conveying device (31) for exhausting the product amplification space (30);
the reagent preparation bin (1), the sample preparation bin (2) and the product amplification bin (3) all comprise air pressure sensors for detecting air pressure values in corresponding spaces.
2. The molecular assay cartridge body structure according to claim 1, wherein the gas pressure in the sample preparation space (20) is greater than the gas pressure in the product amplification space (30).
3. The molecular detection assembly line cartridge structure according to claim 1, wherein the air pressure in the reagent preparing space (10) is equal to or higher than the external air pressure.
4. The molecular detection assembly line bin structure according to claim 1, wherein the difference between the air pressure in the reagent preparing space (10) and the external air pressure is 0-15 pa, the difference between the sample preparing space (20) and the external air pressure is-15 pa to-5 pa, and the difference between the air pressure in the product amplifying space (30) and the air pressure in the sample preparing space (20) is-15 pa to-5 pa.
5. The molecular detection assembly line bin body structure according to claim 1, wherein the reagent preparing bin (1) comprises a first air inlet and a first air outlet which are communicated with the reagent preparing space (10), the first air flow conveying device (11) is communicated with the first air inlet, one of the first air inlet and the first air outlet is arranged above the reagent preparing space (10), and the other is arranged below the reagent preparing space (10);
the sample preparation bin (2) comprises a second air inlet and a second air outlet which are communicated with the sample preparation space (20), the second air flow conveying device (21) is communicated with the second air outlet, the second air inlet is arranged above the sample preparation space (20), and the second air outlet is arranged below the sample preparation space (20);
the product amplification bin (3) comprises a third air inlet and a third air outlet which are communicated with the product amplification space (30), the third air flow conveying device (31) is communicated with the third air outlet, the third air inlet is arranged above the product amplification space (30), and the third air outlet is arranged below the product amplification space (30).
6. The molecular assay cartridge structure of claim 5, wherein
The first air inlet, the first air outlet, the second air inlet, the second air outlet, the third air inlet and the third air outlet are respectively provided with an air purifying device for filtering gas;
the reagent preparing space (10), the sample preparing space (20) and the product amplifying space (30) are internally provided with sterilizing devices for sterilizing the interior, and the sterilizing devices are ultraviolet lamps.
7. The molecular detection pipeline cartridge body structure according to any one of claims 1 to 6, wherein an isolation transfer device (4) is provided between the reagent preparation cartridge (1) and the sample preparation cartridge (2) and/or between the sample preparation cartridge (2) and the product amplification cartridge (3), the isolation transfer device (4) comprising:
a housing (40) provided with a conveying space (400) for accommodating objects to be conveyed, and a first window (402) and a second window (403) which are both communicated with the conveying space (400), wherein the first window (402) and the second window (403) are respectively positioned in two bins;
a first window (41) rotatably connected to the housing (40) for closing the first window (402);
a second window (42) rotatably connected to the housing (40) for closing the second window (403); the method comprises the steps of,
and the opening and closing mechanism is used for driving the first window (41) and the second window (42) to be opened and closed, and when one of the first window (41) and the second window (42) is in an opened state, the other is in a closed state.
8. The molecular detection assembly line cartridge structure according to claim 7, wherein the opening and closing mechanism comprises a linear driver (430), a pallet (431) driven by the linear driver (430) to perform linear motion, a rack (432) connected with the pallet (431), and two sets of windowing components (433), the windowing components (433) comprise a gear (4330) rotatably connected with the housing (40) and a connecting rod (4331) connected with the gear (4330), and the gear (4330) is located on a moving path of the rack (432) and is used for being meshed with the rack (432);
one connecting rod (4331) of the two groups of windowing components (433) is used for jacking the first window body (41) under the drive of the gear (4330), and the other connecting rod (4331) is used for jacking the second window body (42) under the drive of the gear (4330).
9. The molecular detection pipeline bin structure according to claim 8, wherein when the first window (41) and the second window (42) are both in a closed state, the gears (4330) of the two sets of window assemblies (433) are respectively located at two ends of the rack (432), and the rack (432) is meshed with only one set of gears (4330) of the window assemblies (433) at a time.
10. The molecular detection assembly line bin structure according to claim 9, wherein the isolation conveying device (4) further comprises two limiting pieces (45) relatively fixed with the shell (40), the two limiting pieces (45) are respectively located below the first window (41) and the second window (42), and the limiting pieces (45) are used for limiting the lower limit position of the connecting rod (4331).
11. A molecular detection pipeline, characterized by comprising a molecular detection pipeline cartridge structure according to any one of claims 1 to 10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311361047.6A CN117101741B (en) | 2023-10-20 | 2023-10-20 | Molecular detection assembly line storehouse body structure and molecular detection assembly line |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311361047.6A CN117101741B (en) | 2023-10-20 | 2023-10-20 | Molecular detection assembly line storehouse body structure and molecular detection assembly line |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117101741A true CN117101741A (en) | 2023-11-24 |
| CN117101741B CN117101741B (en) | 2023-12-29 |
Family
ID=88813123
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311361047.6A Active CN117101741B (en) | 2023-10-20 | 2023-10-20 | Molecular detection assembly line storehouse body structure and molecular detection assembly line |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117101741B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117551547A (en) * | 2024-01-09 | 2024-02-13 | 四川徕伯益自动化技术有限公司 | Reagent amplification detection equipment and detection method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111747037A (en) * | 2020-06-11 | 2020-10-09 | 朱伟强 | Closed material exchange windowsill capable of preventing virus propagation |
| CN214330097U (en) * | 2021-09-07 | 2021-10-01 | 山东大华医特环保工程有限公司 | Automatic nucleic acid detection integration shelter laboratory goes up and down |
| CN218912384U (en) * | 2022-12-09 | 2023-04-25 | 重庆海润节能技术股份有限公司 | Full-automatic PCR shelter detection laboratory |
| CN116676183A (en) * | 2023-07-31 | 2023-09-01 | 四川徕伯益自动化技术有限公司 | Molecular diagnosis system and molecular diagnosis method |
-
2023
- 2023-10-20 CN CN202311361047.6A patent/CN117101741B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111747037A (en) * | 2020-06-11 | 2020-10-09 | 朱伟强 | Closed material exchange windowsill capable of preventing virus propagation |
| CN214330097U (en) * | 2021-09-07 | 2021-10-01 | 山东大华医特环保工程有限公司 | Automatic nucleic acid detection integration shelter laboratory goes up and down |
| CN218912384U (en) * | 2022-12-09 | 2023-04-25 | 重庆海润节能技术股份有限公司 | Full-automatic PCR shelter detection laboratory |
| CN116676183A (en) * | 2023-07-31 | 2023-09-01 | 四川徕伯益自动化技术有限公司 | Molecular diagnosis system and molecular diagnosis method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117551547A (en) * | 2024-01-09 | 2024-02-13 | 四川徕伯益自动化技术有限公司 | Reagent amplification detection equipment and detection method |
| CN117551547B (en) * | 2024-01-09 | 2024-03-22 | 四川徕伯益自动化技术有限公司 | Reagent amplification detection equipment and detection method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117101741B (en) | 2023-12-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN117101741B (en) | Molecular detection assembly line storehouse body structure and molecular detection assembly line | |
| CN211057123U (en) | Full-automatic nucleic acid extraction and real-time fluorescence quantitative PCR integrated device | |
| CN111560314B (en) | Automatic incubator of stem cells | |
| US10106769B2 (en) | Automatic culturing device | |
| CN115161188B (en) | Air microorganism detection system and method | |
| CN110982685A (en) | Unmanned isolation detection system and method for high-risk pathogenic microorganisms | |
| CN112410204A (en) | Full-automatic nucleic acid detecting system | |
| CN214167961U (en) | Full-automatic nucleic acid detecting system | |
| US20050042710A1 (en) | Sterility sampling test method and apparatus | |
| CN115927550A (en) | Full-automatic nucleic acid sample detection pretreatment method | |
| JP2005304303A (en) | Supply and exhaust robot and automatic apparatus for culture | |
| CN116396850A (en) | Full-automatic nucleic acid sample processing system | |
| JP4835311B2 (en) | Cartridge inspection device | |
| CN112263281A (en) | Negative pressure transmission pathology examination full-flow system for infectious specimen | |
| CN113801770B (en) | Nucleic acid detection pretreatment device | |
| CN219010284U (en) | Automatic detection equipment for sterile environment injection medicines | |
| EP4086333A1 (en) | Fully automatic gene analysis apparatus and gene analysis method | |
| KR102676317B1 (en) | Adapter for mounting conductive pipette, opening and closing apparatus for tube and automatic analysis system | |
| CN113214982B (en) | PCR chamber | |
| CN214334411U (en) | Saliva nucleic acid detection biosafety acquisition processor | |
| CN115505520A (en) | Full-automatic nucleic acid sample processing system | |
| CN115572673A (en) | Full-automatic sample library preparation system and application | |
| CN217989348U (en) | PCR amplification operation cabinet with sample transmission function | |
| JPH06113814A (en) | Biochemical apparatus | |
| CN219730937U (en) | Gene detection assembly line equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |