CN219730937U - Gene detection assembly line equipment - Google Patents

Abstract

The embodiment of the utility model provides gene detection assembly line equipment, which comprises a plate stack module, wherein one outer side face is provided with a first cup separating instrument window, and the other opposite outer side face is provided with a first polymerase chain reaction window; the cup separating instrument module is detachably arranged on the outer side surface of the plate stack module; the pipetting module is detachably arranged on one outer side surface of the plate stack module, which is not provided with a window; the polymerase chain reaction module is detachably arranged on the outer side surface of the first polymerase chain reaction window of the plate stack module; the master control cabinet module is detachably connected with the cup separating instrument module on one outer side surface and the pipetting module on the other outer side surface; the gas pressure in the cup separating instrument module, the sample pretreatment space and the polymerase chain reaction module is gradually decreased. The gene detection assembly line equipment provided by the embodiment of the utility model limits the structural layout of the gene detection assembly line equipment by arranging the three spaces with different air pressures, so that the structural layout is more reasonable, and the size is reduced.

Description

Gene detection assembly line equipment

Technical Field

The utility model relates to the field of machinery, in particular to gene detection assembly line equipment.

Background

The full-automatic assembly line equipment for gene detection comprises a plurality of main modules such as a loading module, a separating cup module, a sample transferring module, a pipetting module, an extraction module, a sealing module, a tearing module, a plate stack warehouse, a transfer window, a polymerase chain reaction instrument and the like, and auxiliary modules such as an uninterruptible power supply, a high-efficiency air filter, a waste liquid storage module, an electric cabinet and the like.

At present, large-flux full-automatic gene detection equipment is large in volume, and relatively small rooms are generally used in places such as laboratories of detection houses, hospitals and the like. Even with a compact layout, the volume is much larger than that of a conventional medical instrument. And the operation flow is complex, and materials are transferred among the modules, so that reasonable layout is needed.

Therefore, how to design a layout which is convenient for the operation of the equipment, improves the flux and reduces the volume becomes a technical problem which needs to be solved by the person skilled in the art.

Disclosure of Invention

To solve the above problems, an embodiment of the present utility model provides a genetic testing pipeline apparatus, including:

the plate stack module is provided with a first cup separating instrument window on one outer side surface and a first polymerase chain reaction window on the other opposite outer side surface;

the cup separating instrument module is detachably arranged on the outer side face of a first cup separating instrument window of the board stack module, and a second cup separating instrument window corresponding to the first cup separating instrument window is arranged on the outer side face of the connection of the cup separating instrument module and the board stack module;

the pipetting module is detachably arranged on the outer side surface of the plate stack module, which is not provided with a window, and the pipetting module is communicated with the plate stack module contact space and is combined into a sample pretreatment space;

the polymerase chain reaction module is detachably arranged on the outer side face of a first polymerase chain reaction window of the plate stack module, and a second polymerase chain reaction window corresponding to the first polymerase chain reaction window is arranged on the outer side face of the connection of the polymerase chain reaction module and the plate stack module;

the master control cabinet module is detachably connected with the cup separating instrument module on one outer side surface and the pipetting module on the other outer side surface;

the air pressure in the cup separating instrument module, the sample pretreatment space and the polymerase chain reaction module is gradually decreased.

Optionally, the method further comprises:

the sample pretreatment space high-efficiency air filter is arranged in the upper space in the master control cabinet module;

the cup separating instrument high-efficiency air filter is arranged outside and above the cup separating instrument module;

the efficient air filter for the polymerase chain reaction space is arranged in the upper space in the polymerase chain reaction module.

Optionally, the cup separating instrument module, the sample pretreatment space and the polymerase chain reaction module are sequentially connected.

Optionally, the gas pressure in the cup separator module, the sample pretreatment space and the polymerase chain reaction module is less than the standard atmospheric pressure.

Optionally, the board stack module further comprises:

a pallet stack module base;

the film tearing instrument is arranged at one side of the upper space in the plate stack module base body;

the film sealing instrument is arranged on the other side of the upper space in the plate stack module base body and is arranged side by side with the film tearing instrument along the direction from the cup separating instrument module to the polymerase chain reaction module;

the mechanical arm is arranged in the base body of the plate stack module, close to the bottom of the pipetting module and positioned between the film tearing instrument and the film sealing instrument;

the plate stack is arranged at the inner bottom of the plate stack module base body and is positioned below the film tearing instrument or the film sealing instrument;

the camera is arranged on the top cover above the manipulator in the board stack module.

Optionally, the board stack module further comprises a film tearing instrument access door and a film sealing instrument access door, which are arranged on an outer side surface of the board stack module substrate opposite to the pipetting module and correspond to the film tearing instrument and the film sealing instrument.

Optionally, the pipetting module includes:

a pipetting module matrix;

the extraction devices are arranged on the inner bottom surface of the matrix of the pipetting module, the number of the extraction devices is at least 2, and the extraction devices are arranged side by side along the direction from the cup separation device module to the polymerase chain reaction module;

and the pipetting station is arranged above the extractor in the pipetting module substrate.

The air channel is arranged above the pipetting module substrate contacted with the master control cabinet module and is used for connecting the sample pretreatment space efficient air filter and the pipetting module;

one end of the air suction pipe is connected with the sample pretreatment space high-efficiency air filter, and the other end of the air suction pipe is close to the extractor;

the reagent input door is arranged on one outer side surface of the pipetting module, which is opposite to the plate stack module.

Optionally, the polymerase chain reaction module comprises:

the polymerase chain reaction module matrix is divided into two parts, namely a first polymerase chain reaction module matrix and a second polymerase chain reaction module matrix, and the polymerase chain reaction space efficient air filter is arranged in the upper space in the second polymerase chain reaction module matrix;

the manipulator is arranged in the first polymerase chain reaction module matrix and close to the bottom surface of the second polymerase chain reaction module matrix;

one of the polymerase chain reaction instruments is arranged on one side, away from the second polymerase chain reaction module substrate, of the manipulator in the first polymerase chain reaction module substrate, and the other two are arranged below the space efficient air filter for the polymerase chain reaction in the second polymerase chain reaction module substrate and are distributed up and down.

Optionally, the master control cabinet module includes:

a master control cabinet module matrix;

the power supply access module is arranged in the lower space in the main control cabinet module base body;

the circuit protection module is arranged in the lower space in the main control cabinet module base body;

the PLC control module is arranged in the lower space in the main control cabinet module base body;

the power supply access module, the circuit protection module and the PLC control module are sequentially connected.

Compared with the prior art, the technical scheme of the embodiment of the utility model has the following advantages:

the gene detection assembly line equipment provided by the embodiment of the utility model comprises: the plate stack module is provided with a first cup separating instrument window on one outer side surface and a first polymerase chain reaction window on the other opposite outer side surface; the cup separating instrument module is detachably arranged on the outer side face of a first cup separating instrument window of the board stack module, and a second cup separating instrument window corresponding to the first cup separating instrument window is arranged on the outer side face of the connection of the cup separating instrument module and the board stack module; the pipetting module is detachably arranged on the outer side surface of the plate stack module, which is not provided with a window, and the pipetting module is communicated with the plate stack module contact space and is combined into a sample pretreatment space; the polymerase chain reaction module is detachably arranged on the outer side face of a first polymerase chain reaction window of the plate stack module, and a second polymerase chain reaction window corresponding to the first polymerase chain reaction window is arranged on the outer side face of the connection of the polymerase chain reaction module and the plate stack module; the master control cabinet module is detachably connected with the cup separating instrument module on one outer side surface and the pipetting module on the other outer side surface; the air pressure in the cup separating instrument module, the sample pretreatment space and the polymerase chain reaction module is gradually decreased. In this way, the gene detection assembly line device provided by the embodiment of the utility model has fewer modules, and the structural layout of the gene detection assembly line device is limited by arranging three spaces with different air pressures, and comprehensively considers the balancing among the beats of each sub-module, and adjacent sub-modules are connected up and down in turn Cheng Mofeng, so that the running distance during transferring is shortened, the structural layout is more reasonable, the device flux can be improved, and the size is reduced.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present utility model, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.

FIG. 1 is a front view showing the structure of a genetic testing pipeline apparatus according to an embodiment of the present utility model;

FIG. 2 is a rear view showing the structure of a genetic testing pipeline apparatus according to an embodiment of the present utility model;

FIG. 3 is an exploded front view of a gene detection pipeline apparatus according to an embodiment of the present utility model;

FIG. 4 is a front view showing another construction of a genetic testing pipeline apparatus according to an embodiment of the present utility model;

FIG. 5 is a front view showing still another construction of a genetic testing pipeline apparatus according to an embodiment of the present utility model;

FIG. 6 is an exploded rear view of another construction of a genetic testing pipeline apparatus provided in an embodiment of the present utility model;

FIG. 7 is an exploded front view of another construction of a genetic testing pipeline apparatus provided by an embodiment of the present utility model;

FIG. 8 is a partial block diagram of a genetic testing pipeline apparatus according to an embodiment of the present utility model;

FIG. 9 is a side view of a portion of the construction of a genetic testing line apparatus provided in an embodiment of the present utility model;

FIG. 10 is a top view showing a part of the construction of a genetic testing pipeline apparatus according to an embodiment of the present utility model;

FIG. 11 is another partial block diagram of a genetic testing pipeline apparatus according to an embodiment of the present utility model;

FIG. 12 is a further view showing another partial structure of a gene assaying line device according to an embodiment of the present utility model;

FIG. 13 is a block diagram of a further portion of a genetic testing pipeline apparatus according to an embodiment of the present utility model;

FIG. 14 is a block diagram of a further portion of a genetic testing pipeline apparatus according to an embodiment of the present utility model.

Detailed Description

As known from the background art, the existing full-automatic gene detection equipment is large in size, inconvenient to transport, complex in structure and difficult to rapidly configure and use.

In order to simplify the design and make the structural layout more reasonable and reduce the size, the embodiment of the utility model provides a genetic testing pipeline device, and the technical scheme in the embodiment of the utility model will be clearly and completely described below with reference to the drawings in the embodiment of the utility model, and obviously, the described embodiment is only a part of embodiments of the utility model, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that the direction or positional relationship referred to in this specification is based on the direction or positional relationship shown in the drawings, and is merely for convenience of description and simplification of description, and does not indicate or imply that the apparatus referred to must have a specific direction, and is configured in a specific direction, and thus should not be construed as limiting the utility model.

Referring to fig. 1 to 2, and fig. 6 to 7, fig. 1 is a front view of an overall structure of a genetic testing pipeline apparatus according to an embodiment of the present utility model, and fig. 2 is a rear view of the genetic testing pipeline apparatus according to an embodiment of the present utility model.

As shown in the figure, the gene detection pipeline device provided by the embodiment of the utility model comprises:

the plate stack module 32, one outer side surface is provided with a first cup separating instrument window 328, and the other opposite outer side surface is provided with a first polymerase chain reaction window 3211;

the cup separating instrument module 2 is detachably and closely connected to the outer side surface of the first cup separating instrument window 328 of the board stack module 32, and the outer side surface of the cup separating instrument module 2 connected with the board stack module 32 is provided with a second cup separating instrument window 24 corresponding to the first cup separating instrument window 328;

the pipetting module 31 is detachably and closely connected to an outer side surface of the plate stack module 32, where the outer side surface is not provided with a window, and the pipetting module 31 and the plate stack module 32 are in contact space intercommunication and combined into a sample pretreatment space 3.

The master control cabinet module 1 has one outer side detachably connected with the cup separating instrument module 2 and the other outer side detachably connected with the pipetting module 31.

It will be readily appreciated that the "first cuvette lid 328, first polymerase chain reaction window 3211, second cuvette lid 24" herein are used to open and transfer reagents when exchange of reagents between modules is required. The first cup dispenser window 328 and the corresponding second cup dispenser window 24 may be in close fit without leakage of air.

It should be noted that, as used herein, "the contact space between the pipetting module 31 and the plate stack module 32 is communicated" means that the contact surface between the pipetting module 31 and the plate stack module 32 has a larger opening or has no sidewall, as shown in fig. 6 to 7, the contact surface between the pipetting module 31 and the plate stack module 32 has no sidewall, and the internal spaces of the two modules are communicated.

It will be readily appreciated that, because the interior spaces of the pipetting module 31 and the plate stack module 32 are in communication, the interior spaces of the pipetting module 31 and the plate stack module 32 can be considered as one closed overall space, and therefore the pipetting module 31 and the plate stack module 32 can also be considered as one whole, i.e. the sample pretreatment space 3.

Therefore, the gene detection assembly line equipment provided by the embodiment of the utility model is designed into a plurality of detachable modules, and each module can be independently used as a whole during assembly and disassembly, so that the design on assembly is simplified, the workload of disassembly and assembly is greatly reduced, and the equipment assembly time is shortened.

Since the genetic testing pipeline apparatus may need to process reagents with high infectivity, in order to avoid leakage of infectious agents, in a specific embodiment, the cup separating instrument module 2 and the sample pretreatment space 3 of the genetic testing pipeline apparatus provided by the embodiment of the utility model are closed spaces.

It is easy to understand that, in order to prevent the contaminated air in the cup dispenser module 2 and the sample pretreatment space 3 from leaking, the cup dispenser module 2 and the sample pretreatment space 3 are both closed spaces, and the internal space can only interact with the outside through the plurality of windows.

Further, in order to facilitate connection between the modules, in a specific embodiment, the cup separating instrument module 2, the sample pretreatment space 3 and the master control cabinet module 1 of the genetic testing pipeline device provided by the embodiment of the utility model are detachably connected through aviation plugs.

The aerial plug described herein is one type of connector, being an electromechanical component that connects electrical lines. The general socket is easy to fall off, and the connector of the aviation plug can be automatically locked after being inserted so as to avoid falling off, so that the aviation plug can assist in fixing when being connected with electronic components.

Like this, through set up the aviation plug that corresponds unanimity each other on different modules, when equipment assembly, the module is assembled in place after inserting aviation plug can, safe convenient has simplified the design to the equipment time has been reduced.

Further, in order to completely implement the gene detection flow, as shown in fig. 3, in a specific implementation manner, the gene detection pipeline device provided by the embodiment of the present utility model further includes: the polymerase chain reaction module 4 is detachably and closely connected to the outer side surface of the first polymerase chain reaction window 3211 of the board stack module 32, and the outer side surface of the polymerase chain reaction module 4 connected with the board stack module 32 is provided with a second polymerase chain reaction window (not shown) corresponding to the first polymerase chain reaction window 3211, and the polymerase chain reaction module 4 is divided into two parts, namely a first polymerase chain reaction module 41 and a second polymerase chain reaction module 42.

The second pcr window 411 may be tightly combined with the corresponding first pcr window 3211, no air leakage occurs, and the first pcr window 3211 and the second pcr window 411 are provided with louvers, which are not opened at the same time to isolate the sample pretreatment space 3 from the pcr space, and a platform between the two louvers may be used to hold the prepared sample.

The gene detection pipeline equipment of the polymerase chain reaction module 4 connected with other modules is shown in fig. 4.

The basic steps of the polymerase chain reaction described herein are: 1. denaturation of template genes: heating the template gene to 93 ℃ for a certain time, and then dissociating the double-stranded template gene or the double-stranded gene formed by polymerase chain reaction amplification to form a single strand so that the single strand is combined with the primer to prepare for the next round of reaction; 2. annealing (renaturation) of template gene and primer: after the template gene is denatured into a single chain by heating, the temperature is reduced to about 55 ℃, and the primer is combined with the complementary sequence of the single chain of the template gene in a pairing way; 3. extension of the primer: under the action of 72 ℃ and gene polymerase, the gene template-primer conjugate takes deoxyribonucleoside triphosphate as a reaction raw material, a target sequence as a template, a new half-reserved copy chain complementary with a template gene chain is synthesized according to the base complementary pairing and half-reserved copy principle, more half-reserved copy chains can be obtained by repeating three processes of cyclic denaturation, annealing and elongation, and the new chain can become the template of the next cycle. Every cycle is completed for 2-4 minutes, and the target gene to be amplified can be amplified for millions of times within 2-3 hours. The molecular biological technology for amplifying specific DNA fragment can be regarded as the special DNA duplication outside organism, and the biggest characteristic of polymerase chain reaction is that trace DNA can be greatly increased. That is, in some gene detection processes, it is necessary to detect a signal of a part of the gene, but in a conventional environment, a signal of a target gene is weak, so that the target gene can be duplicated by polymerase chain reaction so that the target gene can be detected. And finally, judging whether the sample contains the target gene or not through the number of polymerase chain reactions of the target gene after detecting the signal of the target gene.

Because the sample to be tested needs to be processed in the polymerase chain reaction module 4, the interior of the polymerase chain reaction module 4 is also a polluted space, so in a specific embodiment, the polymerase chain reaction module 4 of the gene detection pipeline device provided by the embodiment of the utility model is a closed space.

In addition, in order to facilitate assembly and fixation and unified interface with other modules, in a specific embodiment, the polymerase chain reaction module 4 of the genetic testing pipeline device provided by the embodiment of the utility model is connected with the sample pretreatment space 3 through an aviation plug.

Further, in order to treat the polluted air inside the genetic testing pipeline equipment provided by the embodiment of the present utility model after the use is finished, as shown in fig. 6 to 7, in a specific implementation manner, the genetic testing pipeline equipment provided by the embodiment of the present utility model further includes high-efficiency air filters, the number of which is 3, and is divided into:

the sample pretreatment space efficient air filter 11 is arranged above the inside of the main control cabinet module 1;

the cup separating instrument high-efficiency air filter 23 is arranged outside and above the cup separating instrument module 2;

a pcr space efficient air filter 421 is disposed in the upper space of the pcr module 4.

The standard of the high-efficiency air filter described herein is an air filter that traps particulate dust of 0.5um or more. The air pressure inside the genetic testing pipeline equipment can be reduced by a fan, so that polluted air passes through a high-efficiency air filter and is cleaned.

In the use process of the gene detection assembly line equipment, the risk degree of the air in the cup separating instrument module 2, the sample pretreatment space 3 and the polymerase chain reaction module 4 is sequentially increased, so the gene detection assembly line equipment provided by the embodiment of the utility model is also arranged according to the sequence of the cup separating instrument module 2, the sample pretreatment space 3 and the polymerase chain reaction module 4.

Further, in order to prevent air in the genetic testing apparatus from leaking when the first cuvette lid 328 or the first polymerase chain reaction window 3211 or the second cuvette lid 24 is opened, the direction of air flow when the windows are opened is required to be that the genetic testing apparatus goes to the cuvette lid 2, then to the sample pretreatment space 3, and finally to the polymerase chain reaction module 4. Therefore, in one embodiment, the gas pressures in the cup dispenser module 2, the sample pretreatment space 3, and the polymerase chain reaction module 4 of the genetic testing pipeline apparatus provided by the embodiment of the present utility model decrease sequentially.

Thus, when the first cup separating window 328 or the first polymerase chain reaction window 3211 or the second cup separating window 24 is opened, air flows from outside to inside of the genetic testing pipeline device and finally to the polymerase chain reaction module 4, so that contaminated air in the genetic testing pipeline device is prevented from leaking through the window.

Further, in one embodiment, the gas pressure in the cup dispenser module 2, the sample pretreatment space 3, and the polymerase chain reaction module 4 of the genetic testing pipeline apparatus provided by the embodiment of the utility model is smaller than the standard atmospheric pressure. Specifically, one embodiment may be: the pressure in the cup separating instrument module 2 is minus 6 Pa; the pressure in the sample pretreatment space high efficiency air filter 11 and the sample pretreatment space 3 is minus 10 Pa; the pressure in the space efficient air filter 421 and the polymerase chain reaction module 4 is negative 20 Pa, so that the gas does not leak out of the equipment when the air pressure outside the equipment fluctuates.

In addition, in order to facilitate the movement and transportation of the apparatus, in a specific embodiment, the bottom of the plate stack module 32, the pipetting module 31, the cup separating instrument module 2, the polymerase chain reaction module 4 and the master control cabinet module 1 of the genetic testing pipeline apparatus provided by the embodiment of the utility model is provided with moving rollers.

In the operation of the gene detection assembly line device provided by the embodiment of the utility model, the flux of the device is determined by various reasons, such as the operation time of each sub-module, the waiting time of the sub-module during material transfer, the transfer time and the like, and the flux can be improved by shortening the 3 times. The mechanism design of the gene detection assembly line device provided by the embodiment of the utility model comprehensively considers balancing among beats of all sub-modules, adjacent sub-modules are connected up and down in turn Cheng Mofeng, the running distance during transfer is shortened in layout, and larger flux is realized by using smaller total volume and smaller number of sub-modules. Therefore, in one embodiment, as shown in fig. 6, the genetic testing pipeline apparatus provided by the embodiment of the present utility model is sequentially connected to the cup separating device module 2, the sample pretreatment space 3 and the polymerase chain reaction module 4.

The application flow which can be realized by the gene detection assembly line equipment provided by the embodiment of the utility model is as follows: placing the sample into a cup separating instrument; the cup separating instrument module 2 receives a sample and transmits the sample to the sample pretreatment space 3 through a cup separating instrument window after automatically separating the sample; the sample pretreatment space 3 carries out required treatment on the sample to prepare a sample preparation product capable of carrying out polymerase chain reaction, and the sample preparation product is sent to the polymerase chain reaction module 4 through a polymerase chain reaction window; the polymerase chain reaction module 4 can detect sample preparation and transmit detection result signals to the master control cabinet, and the detection result can be checked through a human-computer interaction device.

In order to facilitate the sample to be placed into the cup separating instrument, as shown in fig. 1, in a specific implementation manner, the gene detection pipeline device provided by the embodiment of the utility model further includes:

the artificial sample input door 21 is arranged at one side of the cup separator module 2 opposite to the plate stack module 32;

an automatic sample input window 22 is disposed on a side of the cup separator module 2 opposite to the master control cabinet module 1.

It should be noted that the manual sample input door 21 is used to manually place samples into the cup separating apparatus, and the automatic sample input window 22 may be connected to the automatic sample conveying module 5 (shown in fig. 3), so that samples can be placed into the cup separating apparatus without requiring manual work.

Thus, in one embodiment, the genetic testing pipeline apparatus provided by the embodiments of the present utility model further comprises an automatic sample delivery module 5 coupled to the automatic sample input window 22.

By means of the automated sample delivery module 5, user contact with the sample can be reduced and hazardous contact can be reduced away from the genetic testing pipeline equipment.

In order to accomplish the above-mentioned flow, as shown in fig. 8, in a specific implementation manner, the genetic testing pipeline apparatus provided by the embodiment of the present utility model further includes:

a pallet stack module base 3213;

a film tearing device 323 arranged at one side of the upper space in the plate stack module base 3213;

the film sealing instrument 322 is arranged on the other side of the upper space in the plate stack module base 3213 and is arranged side by side with the film tearing instrument along the direction from the cup separating instrument module 2 to the polymerase chain reaction module 4;

the manipulator 324 is arranged in the plate stack module base 3213 and close to the bottom of the pipetting module, and is arranged between the film tearing instrument and the film sealing instrument;

a plate stack 321, which is arranged below the film tearing instrument or the film sealing instrument at the bottom in the plate stack module substrate 3213;

a camera 325 is disposed on the top cover of the robot in the pallet module 32.

It is readily understood that the pallet module base 3213 refers to the housing of the pallet module.

The camera 325 is used to monitor the motion in the sample pretreatment space. The manipulator 324, the plate stack 321, the film tearing device 323, the film sealing device 322, the pipetting station 311 (shown in fig. 11), and the two extractors 312 can be seen, which is convenient for the user to remotely monitor.

The pallet stack 321 is used for storing deep hole plates. The film tearing instrument 323 and the film sealing instrument 322 are respectively used for tearing or sealing the film of the deep hole plate. The manipulator 324 is a 6-axis manipulator, and is used for moving a sample from a cup separating instrument window to the pipetting station 311, moving consumables on the plate stack 321 to the pipetting station 311 or the cup separating instrument window, moving reagents in the film sealing instrument 322 on the plate stack 321 to the film tearing instrument 323, moving a reagent pore plate which is well torn by the film tearing instrument 323 to the pipetting station 311, moving a sample at the pipetting station 311 to an extractor, moving a nucleic acid sample which is extracted by the extractor to the pipetting station 311, moving a sample preparation prepared by the pipetting station 311 to the film sealing instrument 322, and moving a sample packaged by the film sealing instrument 322 to the window.

The second cup dispenser window 24 has a down-turned louver, and the first cup dispenser window 328 has only a window and no louver. The slide plate in the cup dispenser module 2 can directly push the window plate to the plate stack module 32 and slide to the position above the window plate, and the manipulator 324 can take the cup-separated sample from the slide plate in the cup dispenser module 2. The robot 324 may also place consumables (e.g., orifice plates, gun tips) on the plate stack 321 onto a sled, which is fed into the cup dispenser module 2.

The 6-axis manipulator is a manipulator with 6 servo motors and can be operated by utilizing the rotation and the movement of X, Y, Z axes, so that the layout of each instrument can be expanded from a plane to a space relative to the traditional truss manipulator, the gene detection assembly line equipment provided by the embodiment of the utility model greatly saves the occupied area, the transfer of materials is more time-saving, and the operation efficiency of the equipment is improved.

In order to facilitate maintenance and replacement of the film tearing device and the film sealing device, and taking and placing of the pallet stack reagent, consumable and waste, as shown in fig. 9, in a specific implementation manner, the gene detection assembly line device provided by the embodiment of the utility model further includes: a film tearing device access door 326 and a film sealing device access door 327, which are disposed on an outer side surface opposite to the pipetting module 31 and correspond to the film tearing device 323 and the film sealing device 322; the plate stack reagent and consumable material feeding door 329 is arranged right below the film tearing instrument access door 326 and right below the film sealing instrument access door 327; the trash can access door 3210 is disposed intermediate the dyestripping access door 326 and the sealer access door 327.

As shown in fig. 9, a sliding plate and a sliding rail which move with the film tearing device 323 and the film sealing device 322 are arranged in the plate stack module 32, so that the film tearing device 323 and the film sealing device 322 can move conveniently; as shown in fig. 10, a slide positioning buckle 3212 is further provided in the board stack module 32, so as to facilitate the fixation of the film tearing device 323 and the film sealing device 322.

11-12, in a specific implementation manner, the gene detection pipeline device provided by the embodiment of the utility model further includes:

pipetting module matrix 316;

the extractors 312 are arranged on the inner bottom surface of the pipetting module matrix 316, and the number of the extractors is at least 2, and the extractors are arranged side by side along the direction from the cup separating instrument module 2 to the polymerase chain reaction module 4;

a pipetting station 311 is disposed within the pipetting module matrix 316 above the extractor.

An air passage 313 provided above the pipetting module substrate 316 in contact with the master control cabinet module, and connecting the sample pretreatment space efficient air filter 11 and the pipetting module 31;

an air suction pipe 314, one end of which is connected with the sample pretreatment space high-efficiency air filter 11, and the other end of which is close to the extractor;

as shown in fig. 6, the reagent feeding gate 315 is disposed on an outer side surface of the pipetting module 31 opposite to the plate stack module 32.

The pipetting module base 316 refers to the housing of the pipetting module. The extractor is used for extracting nucleic acid from the sample, and the pipetting station 311 is used for moving the sample into the deep-well plate in the interior of the extractor and adding a reagent to the sample to prepare a sample preparation.

It should be noted that, the air channel 313 may connect the sample pretreatment space hepa filter 11 and the pipetting station 311, and is used for sucking the polluted air in the pipetting station 311 into the hepa filter, and the air channel 313 is tightly matched with the pipetting module 31 and the sample pretreatment space hepa filter 11, so that air is not leaked. As shown in fig. 12, one end of the air suction pipe 314 is close to the extractor 312, and the other end is connected to the sample pretreatment space hepa filter 11, so as to suck the polluted air around the extractor 312 into the hepa filter, and the air suction pipe 314 is tightly matched with the pipetting module 31 and the sample pretreatment space hepa filter 11, so that no air leakage occurs. The reagent input gate 315 is used to store reagents that need to be added to the sample to the pipetting station 311.

In a specific implementation manner, the gene detection pipeline device provided by the embodiment of the utility model further comprises:

as shown in fig. 7, the pcr module substrate 43 is divided into two parts, namely a first pcr module substrate 431 and a second pcr module substrate 432, and the pcr space-efficient air filter 421 is disposed in the upper space of the second pcr module substrate 432;

as shown in fig. 13, the manipulator 412 is disposed in the first pcr module substrate 431 and is close to the second pcr module substrate 432;

the polymerase chain reaction instrument 413, one is arranged on one side of the manipulator 412, which is far away from the second polymerase chain reaction module matrix 432, in the first polymerase chain reaction module matrix 431;

as shown in fig. 14, two pcr machines 422 are further included, and are disposed below the pcr space-efficient air filter 421 in the second pcr module substrate 432 and arranged up and down.

The polymerase chain reaction module substrate 43 is a housing of the polymerase chain reaction module. The manipulator 412 is a 6-axis manipulator, which can greatly save the occupied area, so that the material transfer can save more time, and the operation efficiency of the equipment can be improved. The manipulator 412 may take sample preparation from the second pcr window 411 and feed the pcr instrument 413 or the pcr instrument 422 for detection.

In order to control or receive signals from the cup dispenser module 2, the sample pretreatment space 3, and the apparatus and the manipulator 412 in the polymerase chain reaction module 4, in a specific embodiment, the genetic testing pipeline apparatus provided in the embodiment of the present utility model further includes: a master control cabinet module matrix; the power supply access module, the circuit protection module and the programmable logic controller control module are arranged in the lower space in the main control cabinet module base body; the power supply access module, the circuit protection module and the programmable logic controller control module are sequentially connected.

Because a 6-axis robot is used within the sample pretreatment space 3 and the polymerase chain reaction module 4, the volumes of the sample pretreatment space 3 and the polymerase chain reaction module 4 can be greatly saved, and thus the volumes of the respective modules can be limited for convenience in passing through the elevator door and the room door.

In a specific embodiment, the width of the pipetting module 31, the plate stack module 32, the cup separating instrument module 2, the polymerase chain reaction module 4 and the total control cabinet module 1 of the genetic testing pipeline equipment provided by the embodiment of the utility model is smaller than 1.2 m, and the depth is smaller than 1.8 m.

It should be noted that, according to the investigation, the cargo lift of the genetic testing assembly line device provided by the embodiment of the utility model can be transported, and the width of the cargo lift after opening the door is at least greater than 1.2 meters and the depth is less than 1.8 meters, so that the size of the device is limited to be less than 1.2 meters and the depth is less than 1.8 meters.

In addition, in order to normalize the genetic testing pipeline apparatus, in a specific implementation manner, the genetic testing pipeline apparatus provided by the embodiment of the present utility model:

the plate stack module 32 is consistent with the contour of the contact surface of the cup separator module 2;

the contact surface outline of the pipetting module 31 and the total control cabinet module 1 is consistent;

the contact surface outline of the cup separating instrument module 2 is consistent with that of the master control cabinet module 1;

the polymerase chain reaction module 4 conforms to the contact surface profile of the sample pretreatment space 3.

Therefore, the assembled gene detection assembly line equipment is more similar to a rectangle, so that the gene detection assembly line equipment is more regular and convenient to use.

Although the embodiments of the present utility model are disclosed above, the present utility model is not limited thereto. Various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the utility model, and the scope of the utility model should be assessed accordingly to that of the appended claims.

Claims (9)

1. A genetic testing pipeline apparatus comprising:

the plate stack module is provided with a first cup separating instrument window on one outer side surface and a first polymerase chain reaction window on the other opposite outer side surface;

the cup separating instrument module is detachably arranged on the outer side face of a first cup separating instrument window of the board stack module, and a second cup separating instrument window corresponding to the first cup separating instrument window is arranged on the outer side face of the connection of the cup separating instrument module and the board stack module;

the pipetting module is detachably arranged on the outer side surface of the plate stack module, which is not provided with a window, and the pipetting module is communicated with the plate stack module contact space and is combined into a sample pretreatment space;

the polymerase chain reaction module is detachably arranged on the outer side face of a first polymerase chain reaction window of the plate stack module, and a second polymerase chain reaction window corresponding to the first polymerase chain reaction window is arranged on the outer side face of the connection of the polymerase chain reaction module and the plate stack module;

the master control cabinet module is detachably connected with the cup separating instrument module on one outer side surface and the pipetting module on the other outer side surface;

the air pressure in the cup separating instrument module, the sample pretreatment space and the polymerase chain reaction module is gradually decreased.

2. A genetic testing pipeline apparatus as in claim 1, further comprising:

the sample pretreatment space high-efficiency air filter is arranged in the upper space in the master control cabinet module;

the cup separating instrument high-efficiency air filter is arranged outside and above the cup separating instrument module;

the efficient air filter for the polymerase chain reaction space is arranged in the upper space in the polymerase chain reaction module.

3. The genetic testing pipeline apparatus of claim 1, wherein the cup dispenser module, the sample pretreatment space, and the polymerase chain reaction module are sequentially connected.

4. The genetic testing pipeline apparatus of claim 1, wherein the gas pressure in the cup separation device module, the sample pretreatment space, and the polymerase chain reaction module is less than the standard atmospheric pressure.

5. The genetic testing pipeline apparatus of claim 4, wherein the pallet stack module further comprises:

a pallet stack module base;

the film tearing instrument is arranged at one side of the upper space in the plate stack module base body;

the film sealing instrument is arranged on the other side of the upper space in the plate stack module base body and is arranged side by side with the film tearing instrument along the direction from the cup separating instrument module to the polymerase chain reaction module;

the mechanical arm is arranged in the base body of the plate stack module, close to the bottom of the pipetting module and positioned between the film tearing instrument and the film sealing instrument;

the plate stack is arranged at the inner bottom of the plate stack module base body and is positioned below the film tearing instrument or the film sealing instrument;

the camera is arranged on the top cover above the manipulator in the board stack module.

6. The genetic testing line apparatus of claim 5, wherein the plate stack module further comprises a film tearing apparatus access door and a film sealing apparatus access door disposed on an outer side of the plate stack module substrate opposite the pipetting module, corresponding to the film tearing apparatus and the film sealing apparatus.

7. A genetic testing pipeline apparatus in accordance with claim 2, wherein the pipetting module comprises:

a pipetting module matrix;

the extraction devices are arranged on the inner bottom surface of the matrix of the pipetting module, the number of the extraction devices is at least 2, and the extraction devices are arranged side by side along the direction from the cup separation device module to the polymerase chain reaction module;

the liquid transferring station is arranged above the extractor in the liquid transferring module substrate;

the air channel is arranged above the pipetting module substrate contacted with the master control cabinet module and is used for connecting the sample pretreatment space efficient air filter and the pipetting module;

one end of the air suction pipe is connected with the sample pretreatment space high-efficiency air filter, and the other end of the air suction pipe is close to the extractor;

the reagent input door is arranged on one outer side surface of the pipetting module, which is opposite to the plate stack module.

8. The genetic testing pipeline apparatus of claim 4, wherein the polymerase chain reaction module comprises:

the polymerase chain reaction module matrix is divided into two parts, namely a first polymerase chain reaction module matrix and a second polymerase chain reaction module matrix, and the polymerase chain reaction space efficient air filter is arranged in the upper space in the second polymerase chain reaction module matrix;

the manipulator is arranged in the first polymerase chain reaction module matrix and close to the bottom surface of the second polymerase chain reaction module matrix;

one of the polymerase chain reaction instruments is arranged on one side, away from the second polymerase chain reaction module substrate, of the manipulator in the first polymerase chain reaction module substrate, and the other two are arranged below the space efficient air filter for the polymerase chain reaction in the second polymerase chain reaction module substrate and are distributed up and down.

9. The genetic testing pipeline apparatus of claim 4, wherein the master control cabinet module comprises:

a master control cabinet module matrix;

the power supply access module is arranged in the lower space in the main control cabinet module base body;

the circuit protection module is arranged in the lower space in the main control cabinet module base body;

the PLC control module is arranged in the lower space in the main control cabinet module base body;

the power supply access module, the circuit protection module and the PLC control module are sequentially connected.