CN111575172A

CN111575172A - Gene detection system

Info

Publication number: CN111575172A
Application number: CN202010630335.7A
Authority: CN
Inventors: 朱滨; 邢军芬; 徐建刚
Original assignee: Shanghai Baio Technology Co ltd
Current assignee: Shanghai Hongtan Biotechnology Co.,Ltd.
Priority date: 2020-07-03
Filing date: 2020-07-03
Publication date: 2020-08-25

Abstract

The invention relates to the technical field of gene detection, and discloses a gene detection system. A gene detection system comprising: a control unit and a reagent consumable unit; a tag identification unit; a constant temperature storage unit; the cabinet body is provided with an air inlet and an air outlet, and an operation table for placing operation appliances is arranged inside the cabinet body; the high-efficiency filtering unit is filled at the air outlet of the cabinet body; a gene detection device; and a turnaround unit. According to the gene detection system, the pipetting assembly and other equipment are integrated on the operating platform in the cabinet body, the liquid is pipetted on the operating platform, and other steps are carried out, so that harmful substances generated in the experimental process are filtered by the high-efficiency filtering unit, the pollution of a reagent by pollutants in the environment can be avoided, and the pollution of the reagent related to biological hazards to the environment can also be avoided.

Description

Gene detection system

Technical Field

The invention relates to the technical field of gene detection, in particular to a gene detection system.

Background

The genetic testing is a process of finding abnormality of a specific genetic material by performing testing and analysis using genetic and molecular biological techniques. In recent thirty years, molecular biology technology has been developed rapidly, and gene detection has been widely applied in clinical examination, precise medical treatment, food inspection and quarantine, species identification and the like.

In general, gene detection is based on gene amplification techniques such as PCR, and includes PCR-fluorescent probe method, PCR-hybridization method, gene chip method, and the like. In a complete genetic testing process, multiple steps are involved, such as sample processing, nucleic acid extraction, PCR amplification, molecular hybridization, signal recognition, detection analysis, and the like.

At present, a professional genetic testing system is a process of integrating all steps in a professional testing laboratory and performing genetic testing in the professional testing laboratory. The gene detection process has a long period, and has requirements on the specialties of a detection laboratory and a detection instrument, and also has professional requirements on a gene detection person, and the gene detection person needs to be familiar with the gene detection operation process, to be skilled in mastering precise laboratory instruments such as a PCR instrument and a hybridization instrument, and to perform professional judgment on a detection result.

Based on the above limitations, gene detection requires many steps, requires high manual operation, requires long complicated steps for simultaneous detection, consumes a large amount of manpower and material resources for the case where a plurality of samples need to be detected simultaneously, and has high detection cost. Furthermore, in the genetic diagnosis process, it is necessary to perform a plurality of treatments such as pipetting and mixing of different kinds of liquids using a pipetting module. The construction, use and maintenance costs of a professional examination laboratory are high, the development of a gene diagnosis experiment is limited by space in the existing hospitals, laboratories and other places, and meanwhile, harmful substances such as product aerosol generated in the gene diagnosis process are easy to diffuse and cause pollution.

Disclosure of Invention

The present invention is made in view of the above problems, and it is an object of the present invention to provide a genetic testing system, which integrates a genetic diagnosis sample and a reagent in a cabinet, can be used for genetic diagnosis in a non-professional laboratory, and can prevent pollution caused by diffusion of aerosol and other substances generated during a genetic diagnosis experiment between different areas in the cabinet and pollution of the aerosol to an experimental environment.

Specifically, the present invention provides a gene detection system comprising:

the control unit is used for controlling the action, behavior and function of each part of the gene detection system by collecting data and carrying out analysis and calculation;

the reagent consumable unit comprises reagents and consumables required for completing gene detection, and labels are arranged on the reagents and the consumables;

a tag identification unit which is connected with the control unit in a communication way and identifies information including the types and positions of the reagents and consumables by identifying the tags;

the constant-temperature storage unit is in communication connection with the control unit and is used for storing reagents and consumables;

the cabinet body is in communication connection with the control unit and is provided with an air inlet and an air outlet, the air inlet is positioned below the air outlet, an operation table is arranged inside the cabinet body and is used for placing appliances related to gene detection operation, and the appliances include but are not limited to a liquid-transferring gun, a gun rack, a gun head box, a garbage can, a test tube rack, a vibration instrument, a centrifugal machine and the like;

the efficient filtering unit is filled at the air outlet and used for filtering the gas flowing from the air inlet to the air outlet in a one-way mode;

the gene detection equipment is in communication connection with the control unit and is turned on, turned off and operated according to the instruction of the control unit; and

the turnover unit is in communication connection with the control unit and comprises a mechanical arm with more than four shafts, clamping jaws capable of grabbing reagent tubes, sample tubes, liquid-moving guns and related consumable materials are arranged on the mechanical arm, the mechanical arm acts according to instructions of the control unit, and tasks such as sample adding, liquid moving and the like required by gene detection and tasks such as placing detection materials in gene detection equipment or taking out the detection materials are completed.

Compared with the prior art, the gene detection system provided by the invention has the advantages that the label unit is arranged to identify the labels on the reagent and the consumable, so that the types, the positions and the like of the reagent and the consumable are obtained, the identified information is sent to the control unit, the control unit sends a control command according to the obtained information, and the mechanical arm acts according to the control command to perform operations such as grabbing, position transferring, liquid transferring and the like of the reagent tube. The gene detection system utilizes the control unit to control the action of the mechanical arm, simplifies required auxiliary equipment and instruments, thereby reducing the personnel required for controlling related equipment in the gene detection work, saving human resources and reducing the detection cost.

Will move the integration of equipment such as liquid subassembly on the operation panel of cabinet body inside, carry out the step such as getting of moving of liquid on the operation panel, with the harmful substance via high-efficient filter unit filtering that produces in the experimentation, can avoid reagent by the pollutant pollution in the environment, also can avoid the pollution of the reagent to the environment that relates to biohazard. In addition, the experimental operation of the invention is integrated in a single cabinet body, thereby facilitating the centralized treatment of pollutants generated in the liquid transfer process and the reaction process. Particularly, according to the cabinet body provided by the invention, the single air inlet and the single air outlet limit the one-way path of air flowing inside the cabinet body, after the air flows through the liquid transfer assembly, harmful substances generated in the liquid transfer process can be taken away, on one hand, pollution caused by diffusion of the harmful substances such as aerosol in the cabinet body is avoided, on the other hand, flowing air is filtered and purified by the high-efficiency filtering unit and then is discharged, and the harmful substances are filtered by the high-efficiency filtering unit, so that the air discharged to the outside of the cabinet body is clean.

Only the circulation of the circulation unit is needed to carry out gene detection, and the requirement on the space of the gene detection system is reduced, so that more devices can be correspondingly installed, and the integrity of the gene detection system is improved. Gene detecting system based on arm has advantages such as easy operation is convenient, required manpower resources are few, is convenient for generally use widely. The gene detection system based on the mechanical arm also has the advantages of high working efficiency and low error rate, and can improve the detection efficiency and the detection accuracy of the gene detection system.

In addition, the high-efficiency filter unit is preferably a high-efficiency filter or an ultra-high-efficiency filter with a filtering grade of not less than 99.9%.

Through research on the existing high-efficiency filtering units of various grades, the high-efficiency filter or the ultra-high-efficiency filter can effectively remove all or most types of harmful aerosol and other substances in gene detection experiments, so that excellent experimental space is provided for the biological experiments.

Preferably, the gene diagnosis system further includes a fan for forming a one-way air path that travels toward the air outlet.

According to this preferred scheme, under the drive of fan, the air that gets into the cabinet body from the air intake passes behind work platform's both sides and discharges from the air outlet, and the microorganism or the harmful substance that the circulation air produced because of the experiment above effectively with work platform take away, then microorganism or harmful substance are filtered and are purified by the high efficiency filter unit of air outlet department, improve the purification efficiency of high efficiency filter unit to cabinet body inside and gene diagnosis system external environment.

The air inlet is arranged at the bottom of the cabinet body, and the air outlet is arranged at the top of the cabinet body.

Because the air intake is seted up in the bottom of the cabinet body for the air current can cover the internal wider range of cabinet, avoids or alleviates some corners and can't be covered by the air current and the remaining problem of harmful substance that produces.

One of the air inlet and the air outlet is arranged on the side wall of the cabinet body, and the other one of the air inlet and the air outlet is arranged on the top end surface or the bottom end surface of the cabinet body.

Because one side in air intake and the air outlet is seted up in cabinet side wall, and the side is seted up in cabinet body terminal surface, so the air current need carry on vertical direction to the conversion of horizontal direction or horizontal direction to vertical direction at the in-process that flows to easily the point of transition appears in the flow process, make things convenient for the air current to realize the conversion of laminar flow to torrent or vortex, make the air current can cover the internal wider range of cabinet.

Further, it is preferable that the operation tool placed on the operation table further includes a lid opening device for automatically opening the lid of each reagent container in the reagent container unit.

According to the preferred scheme, the cover opening device is arranged to automatically perform cover opening processing, so that the automation and the intellectualization of the gene detection system are further improved, and the detection efficiency of the gene detection system is improved.

In addition, as preferred, the turnover unit still includes the AGV dolly, and the arm is located the AGV dolly, and the AGV dolly drives the arm and carries out holistic position transfer.

According to the preferred scheme, the AGV trolley can move with the mechanical arm, so that the mechanical arm can be driven to switch positions among different devices such as a constant-temperature storage unit, an operation table and gene detection equipment, and the mechanical arm can also drive the clamped reagent container to switch positions among different devices. The circulation requirement of a gene detection system in a test laboratory can be met by using a small number of circulation units, so that the number of the circulation units is reduced, the cost is saved, and the space is saved.

Further, as preferred, the turnover unit also includes carrier subassembly, including being used for loading the bracket of a plurality of different specification reagent pipes and being used for holding the carrier of consumptive material respectively.

According to the preferred scheme, the reagent containers can be classified and intensively placed by arranging the carrier assembly, the reagent containers can be accurately taken out of the carrier assembly or placed on the carrier assembly by identifying the positions of the reagent containers on the carrier assembly, the reagent containers can be automatically taken out of the carrier assembly and transferred to the position, manual operation is not needed, the efficiency of taking and placing the reagent containers and transferring the position is greatly improved, and the detection efficiency of the gene detection system is correspondingly improved.

Drawings

FIG. 1 is an overall schematic view of a gene assaying system according to an embodiment of the present invention;

FIG. 2 is a schematic structural view of a cabinet according to an embodiment of the present invention;

FIG. 3 is a schematic structural diagram of a transferring unit according to an embodiment of the present invention;

FIG. 4 is a schematic view of the distribution of components on the console in an embodiment of the invention (for simplicity, only the table top of the console is shown);

FIG. 5 is a schematic view of the distribution of components on an AGV according to an embodiment of the present invention (for simplicity, the robotic arm is omitted).

Description of reference numerals:

1. a cabinet body; 1a, an air inlet; 1b, an air outlet; 2. a high efficiency filtration unit; 3. a fan; 10. an operation table; 11. a bearing surface; 11a, a through hole; 12. an outer edge; 100. a control unit; 200. a reagent container unit; 210. a reagent tube; 220. a PCR tube; 230. a sample tube; 300. a tag identification unit; 400. a constant temperature storage unit; 500. an operating implement; 510. a liquid transferring gun; 520. a cartridge for the gun head; 530. a trash can; 540. uncovering equipment; 541. a reagent tube cap screwing machine; 542. a PCR tube cap opener; 560. a reagent tube rack; 570. a pipette rack; 600. a gene detection device; 700. a turnaround unit; 710. a mechanical arm; 720. a first robot arm; 730. a second mechanical arm; 740. an AGV trolley; 750. a carrier assembly; 751. a reagent carrier; 752. a PCR tube holder; 753. eight connected calandrias; 754. a chip cartridge; 755. and (3) a PCR carrier.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings. The structure and the like of the gene assaying system are schematically shown in simplified form in the drawings.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

An embodiment of the present invention provides a gene assaying system, as shown in fig. 1, including a control unit 100, a reagent vessel unit 200, a cabinet 1 having an operation table 10, and a label recognition unit 300 (see fig. 3) communicatively connected to the control unit 100, a constant temperature storage unit 400, an operation tool 500 (see fig. 4), a gene assaying device 600, and a turnaround unit 700. The control unit 100 is an industrial personal computer, and the operation tool 500 is placed on the operation table 10, and includes a pipette gun 510, a gun head box 520, a PCR carrier 755, and the like, as shown in fig. 4. The control unit 100 is connected to the respective devices (the tag identification unit 300, the constant temperature storage unit 400, the operation tool 500, the gene detection device 600, and the circulation unit 700) in the gene detection system by communication, collects information and data, analyzes and calculates the information and the data, controls the operation of the respective devices (the tag identification unit 300, the constant temperature storage unit 400, the operation tool 500, the gene detection device 600, and the circulation unit 700), controls the operation of the gene detection system, and realizes automation and intellectualization of genes in the gene detection system.

The operation table 10 may be an ultra-clean bench on which the operation tool 500 is placed. The liquid moving operation is carried out on the ultra-clean workbench, so that the pollution of the reagent by pollutants in the environment can be avoided. Alternatively, the operation table 10 may be a biosafety cabinet, and the operation tool 500 is placed in the biosafety cabinet. The method can avoid the pollution of reagents related to biohazard to the environment by carrying out pipetting operation in the biological safety cabinet.

By integrating the pipette gun 510, the tip cassette 520, and the PCR carrier 755 on the table top of the same table 10, the space occupied by a plurality of components in the gene detection system is reduced, and the range of operation of the turnaround unit 700 on the table 10 is also reduced, so that the operation program of the turnaround unit 700 can be simplified, and the operation accuracy of the turnaround unit 700 can be improved. Meanwhile, the pipette gun 510, the pipette tip box 520 and the PCR carrier 755 are integrated on the same operation table 10, so that harmful gas generated in the working process of the gene detection system can be treated in a centralized manner, the pollution of the reagent by pollutants in the environment is further avoided, and the pollution of the reagent related to biohazard to the environment is further avoided.

In particular, as shown in fig. 1 and 2, an air inlet 1a and an air outlet 1b are formed in the cabinet 1, the air inlet 1a is located below the air outlet 1b, the operating table 10 is disposed inside the cabinet 1, and the operating devices 500 such as the pipette gun 510, the pipette tip box 520, and the PCR carrier 755 are placed on the operating table 10. The air outlet 1b is filled with a high-efficiency filtering unit 2, and the high-efficiency filtering unit 2 is used for filtering the gas flowing from the air inlet 1a to the air outlet 1b in a single direction.

Will move liquid equipment integration such as subassembly and on the inside operation panel 10 of cabinet body 1, carry out the step such as getting of moving of liquid on operation panel 10, with the harmful substance that produces in the experimentation via the filtration of high-efficient filter unit 2, can avoid reagent by the pollutant pollution in the environment, also can avoid the pollution of the reagent to the environment that relates to biohazard. According to the cabinet body 1 provided by the invention, the single air inlet 1a and the single air outlet 1b limit the one-way path for air to circulate in the cabinet body 1, after the air flows through the liquid transfer assembly, harmful substances generated in the liquid transfer process can be taken away, on one hand, the pollution caused by the diffusion of the harmful substances such as aerosol in the cabinet body 1 is avoided, on the other hand, the flowing air is filtered and purified by the high-efficiency filtering unit 2 and then is discharged, and the harmful substances are filtered by the high-efficiency filtering unit 2, so that the air discharged to the outside of the cabinet body 1 is clean.

Hereinafter, each component of the gene assaying system will be briefly described.

The high-efficiency filtering unit 2 is a high-efficiency filter or an ultra-high-efficiency filter with the filtering grade of not less than 99.9 percent. Through research on the existing high-efficiency filtering units 2 with various grades, the high-efficiency filter or the ultra-high-efficiency filter with the filtering grade of not less than 99.9% can effectively remove all or most types of harmful aerosol and other substances in gene detection experiments, so that excellent experimental space is provided for the biological experiments.

As shown in fig. 2, the table 10 includes a bearing surface 11 in the center of the table 10. The carrying surface 11 is used for carrying the operating device 500, and the carrying surface 11 is provided with a plurality of through holes 11 a. In the preferred embodiment, at least a portion of the through holes 11a are formed in the center of the console 10, and the through holes 11a formed in the center can facilitate the air flow to pass through the center of the working platform, so as to carry away harmful substances such as nucleic acid aerosol generated by the operating device 500 placed on the console 10 during the experiment, thereby improving the coverage of the air flow, and effectively improving the removal efficiency of the harmful substances.

The gene diagnosis system further comprises a fan 3, and the fan 3 is used for forming a one-way air path which advances towards the air outlet 1 b. Under the drive of fan 3, the air that gets into the cabinet body 1 from air intake 1a passes behind work platform's both sides and discharges from air outlet 1b, and the air of circulation effectively takes away the microorganism or the harmful substance that work platform top produced because of the experiment, and then microorganism or harmful substance are filtered and are purified by high-efficient filter unit 2 of air outlet 1b department, improve high-efficient filter unit 2 to the purification efficiency of cabinet body 1 inside and gene diagnosis system external environment.

In addition, the blower 3 is configured to be opened in advance before the cover of the reagent container unit 200 is opened in response to the opening and closing of the cover of the reagent container unit 200 during pipetting, so that the liquid to be pipetted can be exposed to the air and in the air flow, thereby purging the nucleic acid product aerosol on the surface of the pipetting module and other equipment, keeping the pipetting module and other equipment clean, and avoiding interference with experimental results.

In some embodiments, the high efficiency filter unit 2 includes a fan 3, and the fan 3 is configured to drive a gas flow path formed at the air inlet 1a at an air speed of not less than 0.36 m/s. The setting of high-efficient filter unit 2 can hinder the promotion production of gas flow velocity, in order to ensure the efficiency of getting rid of to the harmful substance of internal environment, needs to improve 3 powers of fan. Experiments show that when the air speed at the air inlet 1a is not lower than 0.36m/s, the removal efficiency of harmful substances can be obviously improved. More preferably, the wind speed at the wind inlet 1a is not lower than 0.5 m/s.

In this embodiment, the installation position of the fan 3 is not limited, and may be adjusted as needed, for example, the fan may be installed adjacent to the high efficiency filter or the ultra high efficiency filter of the high efficiency filter unit 2, may be installed adjacent to the air inlet 1a, or may be installed at any other suitable position.

In some embodiments, the fan 3 is further connected with an air flow rate control device, by which the air flow rate inside the cabinet 1 can be adjusted as desired. The air flow rate control device may be, for example, an electrical component that adjusts the power of the fan 3, or may be a mechanical device that adjusts the size of the air outlet of the fan 3, as long as the flow rate of air output by the fan 3 can be controlled as needed.

In some embodiments, the air inlet 1a may be opened at the bottom of the cabinet 1, and the air outlet 1b is opened at the top of the cabinet 1. Because air intake 1a sets up in the bottom of the cabinet body 1 for the air current can cover the bigger scope in the cabinet body 1, avoids or alleviates some corners can't be covered by the air current and the remaining problem of harmful substance that produces.

Of course, in some other embodiments of the present invention, one of the air inlet 1a and the air outlet 1b is opened at a side wall of the cabinet 1, and the other is opened at a top end surface or a bottom end surface of the cabinet 1. Because one of the air inlet 1a and the air outlet 1b is arranged on the side wall of the cabinet body 1, and one of the air inlet and the air outlet is arranged on the end surface of the cabinet body 1, the air flow needs to be converted from the vertical direction to the horizontal direction or from the horizontal direction to the vertical direction in the flowing process, so that the transition point is easy to appear in the flowing process, the laminar flow is converted into the turbulent flow or the vortex flow, and the air flow can cover a larger range in the cabinet body 1.

Referring to fig. 1, the reagent container unit 200 is composed of a plurality of reagent containers including a plurality of reagent tubes 210, PCR tubes 220, and sample tubes 230, wherein labels are disposed on the reagent tubes 210, PCR tubes 220, and sample tubes 230 (the labels are preferably disposed on the outer surfaces of the reagent tubes 210, PCR tubes 220, and sample tubes 230, and are not shown in the figure for simplicity). The label recognition unit 300 recognizes the label to recognize information such as the type and position of each reagent container (i.e., the reagent tube 210, the PCR tube 220, and the sample tube 230). The constant-temperature storage unit 400 is used to store the respective reagent tubes 210, PCR tubes 220, and sample tubes 230, and generally, the reagents in the reagent tubes 210 are stored at a temperature of 2-8 ℃ in the constant-temperature storage unit 400. The manipulator 500 transfers the liquid in each of the reagent tube 210, the PCR tube 220, and the sample tube 230 by using the pipette gun 510, thereby transferring a small amount of the liquid. The gene testing apparatus 600 may be a PCR instrument, a chip tester, etc. for performing PCR amplification, hybridization, signal recognition and reading on the processed sample (blood), and outputting a test report. The transfer unit 700 includes a robot arm 710, and the robot arm 710 is used to transfer the reagent tubes 210, the PCR tube 220, and the sample tube 230, and operate the pipette gun 510.

The plurality of reagent tubes 210 are filled with amplification solutions, amplification reaction solutions, hybridization buffer solutions, hybridization reaction solutions, color developing solutions, etc. required for PCR amplification, hybridization, and color development, respectively.

The workflow of the gene detection system is briefly described as follows: the reagent tubes 210 storing various reagents are stored in the constant temperature storage unit 400. When the gene testing is started, the control unit 100 controls the robot arm 710 to take the reagent tube 210 from the constant-temperature storage unit 400 and then mix different reagents using the pipette gun 510. The robot 710 transfers the reagent tube 210 in which the plurality of reagents are mixed and the PCR tube 220 to which the sample (blood) is added to the gene assaying device 600 through the robot 710 to assay, and then each reagent container after the assay is taken out through the robot 710. The gene assaying device 600 outputs the results of the gene assay.

In the operation process of the robot arm 710, the robot arm is linked with the label recognition unit 300 through the control unit 100, the label recognition unit 300 recognizes information such as the types and positions of the reagent vessels 210 and the PCR vessel 220 in the reagent vessel unit 200, and then transfers the recognized information to the control unit 100, the control unit 100 analyzes and calculates according to the received information, calculates an operation program of the robot arm 710, and sends a control command to the robot arm 710, and the robot arm 710 operates according to the control command.

In the gene detection system according to the present embodiment, the control unit 100, the label recognition unit 300, and the circulation unit 700 are provided, and the label unit can recognize the labels on the reagent tubes 210 and the PCR tubes 220 to obtain the types, positions, and the like of the reagent tubes 210 and the PCR tubes 220, and then transmit the recognized information to the control unit 100, the control unit 100 calculates the operation program of the robot arm 710 based on the obtained information and transmits a control command, and the robot arm 710 operates according to the control command to perform operations such as grasping, position transfer, and liquid transfer of the reagent tubes 210 and the PCR tubes 220. The gene detection system utilizes the control unit 100 to control the action of the mechanical arm 710, automatically performs the work of the gene detection system, and can simplify required auxiliary equipment and instruments, thereby reducing the personnel required for controlling related equipment in the gene detection work, saving human resources and reducing the detection cost. Only the turnover unit 700 is needed for circulation and gene detection, and the requirement on the space of the gene detection system is reduced, so that more devices can be correspondingly installed, and the integrity of the gene detection system is improved. Gene detecting system based on arm 710 has advantages such as easy operation is convenient, required manpower resources are few, is convenient for generally use widely. The gene detection system based on the mechanical arm 710 also has the advantages of high working efficiency and low error rate, and can improve the detection efficiency and the detection accuracy of the gene detection system.

Hereinafter, some devices in the gene assaying system will be described in detail.

The tag recognition unit 300 is a camera or a video camera, the tag recognition unit 300 is disposed on the robot arm 710, and more preferably, the tag recognition unit 300 is embedded on the grasping end of the robot arm 710. The label recognition unit 300 can change positions by the movement of the robot arm 710, recognize labels on the respective reagent containers at multiple angles to acquire information, and improve the accuracy of acquiring information. The label recognition unit 300 is arranged at the grabbing end of the mechanical arm 710, so that the image acquisition of the label recognition unit 300 can be prevented from being blocked due to the arrangement of the mechanical arm 710. The tag identification unit 300 is integrated on the mechanical arm 710, and a transmission mechanism is not separately arranged to drive the tag identification unit 300 to act, so that the number of parts of the gene detection system can be reduced, and the structure of the gene detection system can be simplified.

In another embodiment of the present invention, the tag identification unit 300 may also change its position by using a three-axis linkage mechanism, which is in communication connection with the control unit 100 and operates under the control of the control unit 100 to drive the tag identification unit 300 to operate.

In particular, the tag is a two-dimensional code or a radio frequency tag, which are advantageous. The two-dimensional code can store much information, so that information such as the kind, model, number, time, and the like of the reagent container can be obtained from the label identifying the type of the two-dimensional code. Referring to fig. 3, the robot 710 of the turnaround unit 700 has two robot arms, i.e., a first robot arm 720 and a second robot arm 730, and the label recognition unit 300 also has two robot arms, i.e., a first robot arm 720 and a second robot arm 730, respectively, and is disposed at the ends of the first robot arm 720 and the second robot arm 730, respectively. Through the matching of the label recognition unit 300, the circulation unit 700 and the control unit 100, the first mechanical arm 720 and the second mechanical arm 730 can stably grasp the reagent tube 210 and the PCR tube 220, and the operations of taking and placing the reagent tube 210 and the PCR tube 220 and transferring the positions are accelerated, so that the efficiency of transferring the reagent tube 210 and the PCR tube 220 is improved.

Referring to fig. 4, the manipulation tool 500 placed on the manipulation stage 10 further includes a trash can 530, and the trash can 530 is used to collect tips of the pipette tips 510 after each pipetting. The garbage bin 530 can retrieve the rifle head after the liquid is transferred, and the rifle head after the liquid is transferred is concentrated and is collected and handled, can avoid the rifle head after the liquid is transferred to be laid everywhere and the polluted environment. The garbage can 530 recovers the gun heads in real time, so that the subsequent work of picking up the gun heads can be reduced, the time is saved, and the detection efficiency of the gene detection system is greatly improved.

The manipulation tool 500 placed on the manipulation stage 10 further includes a lid opening device 540 for automatically opening the lid of each of the reagent vessels 210, the PCR vessel 220, and the like in the reagent container unit 200. Through setting up uncapping equipment 540, automatically, open the lid of reagent pipe 210 and PCR pipe 220 etc. and handle uncapping, the automation and the intellectuality that have further improved gene detection system have again been managed 210 and the automation of PCR pipe 220, and gene detection system's efficiency obtains improving. The cap removing apparatus 540 has two kinds, i.e., a reagent tube cap screwing machine 541 for opening and closing the caps of the reagent tubes 210 and the sample tubes 230 and a PCR tube cap removing machine 542 for opening and closing the caps of the PCR tubes 220, because the cap structures of the reagent tubes 210 and the PCR tubes 220 are different.

The handling tool 500 placed on the operation table 10 further includes a reagent tube rack 560 for temporarily storing the reagent tubes 210 during the transfer of the reagent tubes 210.

The handling apparatus 500 placed on the console 10 further includes a pipette rack 570 for holding the pipette guns 510 for storage of the pipette guns 510.

Referring to fig. 1 and 3, the transfer unit 700 further includes an AGV cart 740, the robot arm 710 is disposed on the AGV cart 740, and the AGV cart 740 drives the robot arm 710 to perform overall position transfer. The AGV cart 740 can move with the robot arm 710 to switch positions with the robot arm 710 among different devices such as the constant temperature storage unit 400, the operation table 10, and the gene assaying device 600, and can also switch positions with the robot arm 710 with the reagent vessels 210 and the PCR vessel 220 among different devices. In this embodiment, a transfer unit 700, including an AGV cart 740 and a robotic arm 710 thereon, can satisfy the transfer requirements of a gene testing system in a testing laboratory, thereby reducing the number of transfer units 700, saving cost and space.

Referring to fig. 4 and 5, the turnaround unit 700 further includes a carrier assembly 750, the carrier assembly 750 includes a reagent rack 751 and a PCR tube rack 752 for loading a plurality of reagent tubes 210 and PCR tubes 220, respectively, and the PCR tube rack 752 is used for holding the PCR carrier 755. The carrier assembly 750 further includes an eight-row tube 753 and a chip box 754, wherein the eight-row tube 753 is used for carrying the mixed reagent and sample (blood) and entering the gene testing apparatus 600 for gene testing, and the chip box 754 stores gene chips. By providing the carrier module 750, the reagent cartridge 210, the PCR cartridge 220, the sample cartridge 230, the gene chip, etc. can be classified and collectively placed, by identifying the positions of the reagent vessels 210, the PCR vessel 220, the sample vessel 230, and the gene chip on the carrier module 750, the reagent vessels 210, the PCR vessel 220, the sample vessel 230, and the gene chip can be accurately taken out from the carrier module 750, or the reagent tubes 210, the PCR tubes 220, the sample tubes 230 and the gene chips are put back on the corresponding carrier assemblies 750, so that the taking and the position transfer of the reagent tubes 210, the PCR tubes 220, the sample tubes 230 and the gene chips relative to the carrier assemblies 750 can be automatically realized, manual operation is not needed, the efficiency of the taking and the position transfer of the reagent tubes 210, the PCR tubes 220, the sample tubes 230 and the gene chips is greatly improved, and the detection efficiency of the gene detection system is correspondingly improved.

A label is also provided on the reagent carriage 751, and the label recognition unit 300 is configured to acquire an image of the constant-temperature storage unit 400 removed from the access port, thereby determining the position of the reagent carriage 240. Therefore, the operation of the mechanical arm 710 is convenient, the reagent bracket 751 can be automatically taken and placed, and the taking and placing efficiency of the reagent bracket 751 and the upper reagent tube 210 is improved.

In particular, referring to FIG. 5, there are also empty spaces on the AGV cart 740 to allow placement of reagent trays 751, PCR tube trays 752, eight-row tubes 753, and chip cartridges 754, etc. By the arrangement, the steps of transferring the PCR carrier 755, the eight-row pipe 753 and the chip box 754 can be reduced, the efficiency of driving the mechanical arm 710 to transport at a time by the AGV car 740 is improved, and the stability of the reagent bracket 751, the PCR pipe bracket 752, the eight-row pipe 753 and the chip box 754 in the transportation process is also improved.

In this embodiment, the gene detection device 600 is an integrated machine, which can realize the functions of amplification, hybridization, gene detection, analysis, and the like, and reduce the number of devices such as a PCR instrument, a hybridization instrument, and a recognition instrument, which need to be separately installed.

It will be appreciated by those of ordinary skill in the art that in the embodiments described above, numerous technical details are set forth in order to provide a better understanding of the present application. However, the technical solutions claimed in the claims of the present application can be basically implemented without these technical details and various changes and modifications based on the above-described embodiments. Accordingly, in actual practice, various changes in form and detail may be made to the above-described embodiments without departing from the spirit and scope of the invention.

Claims

1. A gene testing system, comprising:

2. The gene detection system of claim 1, wherein the high-efficiency filter unit is a high-efficiency filter or an ultra-high-efficiency filter with a filter grade of not less than 99.9%.

3. The gene assaying system according to claim 1 or 2, further comprising:

and the fan is used for forming a one-way air path advancing towards the air outlet.

4. The gene detection system of claim 1, wherein the air inlet is formed in the bottom of the cabinet body, and the air outlet is formed in the top of the cabinet body.

5. The gene detecting system according to claim 4, wherein one of the air inlet and the air outlet is provided on a side wall of the cabinet body, and the other one of the air inlet and the air outlet is provided on a top end surface or a bottom end surface of the cabinet body.

6. The gene detection system of claim 1, wherein the tag identification unit is located at an operating end of the turnaround unit robot.

7. The gene assaying system according to claim 1, wherein the operation instrument placed on the operation table further comprises:

and the cover opening device is used for automatically opening the cover of each reagent container in the reagent container unit.

8. The gene detection system of claim 1, wherein the turnaround unit further comprises:

the AGV trolley, the arm is located on the AGV trolley, the AGV trolley drives the arm moves to the assigned position according to the central control system instruction, and the turnover operation is implemented by the arm.

9. The gene detection system of claim 8, wherein the turnaround unit further comprises:

the carrier assembly comprises a bracket and a carrier, wherein the bracket is used for loading a plurality of reagent tubes with different specifications, and the carrier is used for containing consumables.