CN117064011A - Feed additive for preventing diarrhea of weaned pigs as well as preparation method and application thereof - Google Patents
Feed additive for preventing diarrhea of weaned pigs as well as preparation method and application thereof Download PDFInfo
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- CN117064011A CN117064011A CN202311210633.0A CN202311210633A CN117064011A CN 117064011 A CN117064011 A CN 117064011A CN 202311210633 A CN202311210633 A CN 202311210633A CN 117064011 A CN117064011 A CN 117064011A
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- 206010012735 Diarrhoea Diseases 0.000 title claims abstract description 43
- 239000003674 animal food additive Substances 0.000 title claims abstract description 37
- 241000282887 Suidae Species 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 45
- 239000000843 powder Substances 0.000 claims abstract description 28
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims abstract description 27
- 238000002156 mixing Methods 0.000 claims abstract description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000002904 solvent Substances 0.000 claims abstract description 10
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000007873 sieving Methods 0.000 claims abstract description 9
- 238000010298 pulverizing process Methods 0.000 claims abstract description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 13
- 238000002390 rotary evaporation Methods 0.000 claims description 8
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 229940079593 drug Drugs 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 238000003786 synthesis reaction Methods 0.000 abstract description 2
- 230000002155 anti-virotic effect Effects 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 23
- 239000001606 7-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-5-hydroxy-2-(4-hydroxyphenyl)chroman-4-one Substances 0.000 description 12
- DFPMSGMNTNDNHN-ZPHOTFPESA-N naringin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC=2C=C3O[C@@H](CC(=O)C3=C(O)C=2)C=2C=CC(O)=CC=2)O[C@H](CO)[C@@H](O)[C@@H]1O DFPMSGMNTNDNHN-ZPHOTFPESA-N 0.000 description 12
- 229940052490 naringin Drugs 0.000 description 12
- 229930019673 naringin Natural products 0.000 description 12
- 230000000694 effects Effects 0.000 description 7
- 230000036039 immunity Effects 0.000 description 7
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
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- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 238000004090 dissolution Methods 0.000 description 5
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- 239000003795 chemical substances by application Substances 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000011787 zinc oxide Substances 0.000 description 3
- 241000282898 Sus scrofa Species 0.000 description 2
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- 230000000840 anti-viral effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 208000005577 Gastroenteritis Diseases 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- 239000003833 bile salt Substances 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
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- 230000037396 body weight Effects 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
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- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
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- 229960002442 glucosamine Drugs 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
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- 230000007774 longterm Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
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- 239000000126 substance Substances 0.000 description 1
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- 238000010998 test method Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to a feed additive, in particular to a feed additive for preventing diarrhea of weaned pigs, and a preparation method and application thereof, comprising the following steps: s1: dissolving pericarpium Citri Tangerinae powder in extractive solution prepared by mixing anhydrous ethanol and acetic acid to obtain pericarpium Citri Tangerinae powder solution; dissolving chitosan oligosaccharide in an extracting solution formed by mixing absolute ethyl alcohol and acetic acid to obtain a chitosan oligosaccharide solution; s2: mixing pericarpium Citri Tangerinae powder solution with chitosan oligosaccharide solution, removing solvent, pulverizing, and sieving; s3: and (3) uniformly mixing the crushed product obtained in the step (S2) with saccharicterpenin to obtain the feed additive. Compared with the prior art, the invention plays the roles of antivirus and bacteriostasis through raw material mixing, effectively prevents diarrhea of piglets, improves daily gain of weaned piglets, reduces death rate of weaned piglets, and simultaneously solves the problem that the prior art interrupts the use of chemical synthesis medicines for preventing diarrhea of the weaned piglets so as to easily generate environmental pollution.
Description
Technical Field
The invention relates to a feed additive, in particular to a feed additive for preventing diarrhea of weaned pigs, and a preparation method and application thereof.
Background
With the development of modern breeding industry, the scale of specialized and intensive breeding production industry is growing day by day, the early weaning of piglets becomes an important breeding means, and the diarrhea rate of weaned piglets is about 20-30% according to investigation. In general, the diarrhea rate is 0.6% 3-5 days after weaning; diarrhea becomes serious in 8-13 days, the diarrhea rate reaches about 32%, and the death rate caused by the diarrhea can reach 10-20%. Even if the disease is healed, the growth and development of the disease can be seriously affected, the time for leaving the fence is delayed, and huge economic loss is brought to the raisers. The disease is one of the causes of diarrhea in weaned pigs. Common diseases causing diarrhea in weaned pigs are mainly viral and bacterial diseases. The viral diseases mainly comprise transmissible gastroenteritis of swine, epidemic diarrhea, swine fever and the like.
The existing feed additives for diarrhea of piglets are not special and effective, and most of the existing feed additives use chemical synthetic drugs such as zinc oxide along with the forbidden antibiotics, but the long-term use of the feed additives can cause environmental pollution; in addition, some biological agents exist, but microorganisms exist in the microecological agent product, and in the production, transportation and storage processes, the activity of the microorganisms is easily affected by environmental factors such as temperature, moisture, pH value and the like, and the microorganisms are easily damaged by gastric acid, bile salt and digestive enzymes after entering the digestive tract of animals, so that the efficacy of the microbial agent product cannot be exerted, and the application effect of the microbial agent product in production practice is extremely unstable.
Although naringin has the functions of resisting oxidation and improving the immunity and antibacterial capability of animal bodies, naringin extracted by the existing mature naringin extraction technology has low solubility, bitter taste and poor palatability, and the naringin directly mixed into feed can influence the normal feeding of piglets.
Therefore, the preparation method is particularly important for preparing the feed additive which is safe, has no side effect and has stable effect and is used for preventing diarrhea on piglets.
Disclosure of Invention
The invention aims to solve at least one of the problems and provide a feed additive for preventing diarrhea of weaned pigs, a preparation method and application thereof, which solve the problem that the weaned pigs are easy to produce environmental pollution due to diarrhea prevention by adopting chemical synthesis drugs in the prior art, and realize reduction of the use of the feed additive with pollution and reduction of the death rate of the weaned pigs.
The aim of the invention is achieved by the following technical scheme:
the invention discloses a preparation method of a feed additive for preventing diarrhea of weaned pigs, which comprises the following steps:
s1: dissolving pericarpium Citri Tangerinae powder in extractive solution prepared by mixing anhydrous ethanol and acetic acid to obtain pericarpium Citri Tangerinae powder solution; dissolving chitosan oligosaccharide in an extracting solution formed by mixing absolute ethyl alcohol and acetic acid to obtain a chitosan oligosaccharide solution;
s2: mixing pericarpium Citri Tangerinae powder solution with chitosan oligosaccharide solution, removing solvent, pulverizing, and sieving;
s3: and (3) uniformly mixing the crushed product obtained in the step (S2) with saccharicterpenin to obtain the feed additive.
Preferably, in the step S1, the mass ratio of the orange peel powder to the chitosan oligosaccharide is 10:8, the dosage ratio of the orange peel powder to the extracting solution is 10g:200mL, the dosage ratio of chitosan oligosaccharide to the extracting solution is 8g:200mL.
Preferably, in step S1, in the extract solution formed by mixing the anhydrous ethanol and the acetic acid, the volume ratio of the anhydrous ethanol to the acetic acid is 9:1.
preferably, in step S2, after mixing the orange peel powder solution with the chitosan oligosaccharide solution, the pH is adjusted to be alkaline.
Preferably, naOH is used to adjust the pH to 12.0.
Preferably, in step S2, the solvent is removed by means of rotary evaporation and vacuum drying.
It is further preferred that the temperature of rotary evaporation is 45 ℃ to remove most of the solvent in the solution, and then the temperature of vacuum drying is 60 ℃ to completely dry.
Preferably, in step S2, a 100 mesh screen is used for sieving.
Preferably, in step S3, the pulverized product is mixed with saccharicterpenin in equal mass ratio.
The invention discloses a feed additive for preventing diarrhea of weaned pigs, which is prepared by adopting any one of the methods.
The invention in a third aspect discloses an application of the feed additive for preventing diarrhea of weaned pigs, which comprises the following steps: 1000-2500 mass ratio, namely a feed additive: feed = 1:1000-2500 (m: m).
The orange peel powder is rich in naringin, and naringin can be extracted by dissolving the orange peel powder in absolute ethanol and acetic acid; naringin has antioxidant effect, and can improve immunity and resist pathogenic bacteria. The chitosan oligosaccharide is a sugar chain formed by connecting 2-20 glucosamine through beta-1, 4-glycosidic bond, and has good biodegradation, antioxidation and antibacterial activity. The naringin and the chitosan oligosaccharide ligand are combined through covalent bond or hydrogen bond, so that the solubility can be improved, the dissolution and extraction of naringin can be enhanced, and the synergistic enhancement effect can be achieved.
After the orange peel powder and the chitosan oligosaccharide are compatible and dissolved, the pH value is adjusted to be alkaline, so that the sweetness of naringin can be improved, the palatability of naringin is further improved, and the piglets are prevented from interfering with the feed to eat abnormally.
The oligosaccharide in the saccharicterpenin can be selectively utilized by beneficial bacteria such as lactobacillus and bifidobacterium in intestinal tracts, so that the digestion and absorption capacity of the intestinal tracts is improved, the growth of cultured animals is promoted, and the saponin in the saccharicterpenin has good anti-inflammatory and antibacterial effects and also has antiviral effects. The compound of the orange peel powder and the chitosan oligosaccharide is matched with the saccharicterpenin after rotary evaporation, so that the compound has antiviral and antibacterial effects and double-tube effect on diarrhea of piglets.
Compared with the prior art, the invention has the following beneficial effects:
1. the solubility of naringin in the orange peel powder can be effectively improved by utilizing the interaction of the orange peel powder and the chitosan oligosaccharide, so that the antibacterial effect and the immunity improving effect of the orange peel powder and the chitosan oligosaccharide are improved;
2. the components are simple, the use is convenient, and the stability is high;
3. can effectively prevent diarrhea of piglets, improve daily gain of weaned piglets and reduce death rate;
4. the zinc oxide is reduced by the feed additive, so that the pollution to the environment caused by the discharge of the zinc oxide is reduced.
Detailed Description
The invention is described in detail below in connection with specific examples, but in no way limits the invention.
In the examples below, the reagents used, unless otherwise specified, may be conventional commercial products in the art, and the methods employed may be selected by means well known in the art.
Examples
The components are weighed for standby:
orange peel powder: 10g;
absolute ethanol (analytically pure): 500mL;
acetic acid (analytically pure): 500mL;
NaOH：1mol/L；
chitosan oligosaccharide: 8g;
sugar terpene extract: 50g.
The specific use amount is determined according to the required addition amount of the test.
Weighing 10g of orange peel powder in a 500mL beaker, and then adding 200mL of an extraction solution mixed by absolute ethyl alcohol and acetic acid (volume ratio is 9:1) for full dissolution; weighing 8g of chitosan oligosaccharide, adding 200mL of absolute ethyl alcohol and acetic acid (volume ratio is 9:1) into a 500mL beaker, and fully dissolving; mixing the dissolved orange peel powder and chitosan oligosaccharide, regulating the pH value to 12.0 by using NaOH, removing most of the solvent by rotary evaporation of the compound at 45 ℃, drying in vacuum at 60 ℃, and crushing and sieving by a 100-mesh sieve to obtain a product; the crushed product and the saccharicterpenin are mixed according to the weight ratio of 1:1 are used in equal amounts in combination.
Application example
300 weaned piglets in the same batch are selected, and the additive prepared by the embodiment is added according to the proportion of 1: 1000. 1:1500:1:2000 and 1:2500 in mass ratio to the formula feed, and a group of control groups without the feed additive is additionally arranged, wherein the total number of the control groups is 5, each group is divided into 3 parallel tests, and each parallel test is divided into 20; the feeding time is 21d, and diarrhea, weight gain and death and panning of weaned pigs are observed and counted during the feeding period.
The statistical results are shown in table 1, and it can be seen from table 1 that the diarrhea rate of weaned pigs can be effectively reduced by adding the feed additive, wherein 1:2000 was added at a significantly higher rate than the control and 1:1000 groups (P < 0.01), and significantly higher than 1:1500 groups and 1:2500 groups (P < 0.05). 1: the weaned pigs with 2000 addition ratio have the lowest diarrhea number and diarrhea rate and the highest average daily gain.
Thus 1:2000 was added in the optimum ratio.
In table 1, the calculation modes of each item are:
diarrhea rate = number of diarrhea heads of piglets/total number of piglets tested;
average daily gain= (test end body weight-initial body weight)/test days;
mortality = number of dead heads/total days of trial x 100%.
Table 1 statistics of diarrhea status of weaned pigs at different addition ratios
Note that: the acronyms are identical and indicate that there is no significant difference in comparison between groups (P > 0.05); the shoulder letters are different and there is a significant difference in contrast between adjacent presentation groups (P < 0.05); the different and non-adjacent shoulder letters represent a very significant difference (P < 0.01).
Randomly selecting 20 weaned pigs of each group, extracting 10mL of jugular venous blood of the weaned pigs, centrifuging for 10min by a low-temperature centrifugal machine of 3 000r/min to obtain serum, measuring immune indexes including IgG, igA and IgM in the serum by an enzyme-linked immunosorbent assay (ELLSA) method by using a kit, and taking the average value; the kit is purchased from Nanjing to build a bioengineering institute. The measured index data is subjected to statistical analysis by using SPSS22.0 software, and meanwhile, multiple comparison is carried out by combining with Duncan's method, the difference significance among treatments is checked, P <0.05 represents the difference significance, and P <0.01 represents the difference and significance; the test results are all expressed as mean ± standard deviation.
The test results are shown in Table 2, and from Table 2, it can be seen that 1:1000 groups, 1:1500 groups, 1:2000 groups and 1: the 2500 groups had higher IgA, igG and IgM contents than the control group without feed additive, and were rated at 1: the IgA, igG and IgM content of group 2000 was highest, significantly higher than control and 1:1000 groups (P < 0.01), and significantly higher than 1:1500 groups and 1:2500 groups (P < 0.05).
TABLE 2 influence of different addition ratios on serum immunity of weaned pigs
| Group of | Control group | 1:1000 groups | 1:1500 set | 1: group 2000 | 1:2500 group |
| IgA | 0.609±0.004 a | 0.634±0.005 a | 0.684±0.010 b | 0.728±0.009 c | 0.681±0.011 b |
| IgG | 2.247±0.005 a | 2.263±0.004 a | 2.301±0.021 b | 2.389±0.016 c | 2.344±0.012 b |
| IgM | 0.164±0.010 a | 0.170±0.016 a | 0.181±0.031 b | 0.203±0.008 c | 0.184±0.014 b |
Note that: the acronyms are identical and indicate that there is no significant difference in comparison between groups (P > 0.05); the shoulder letters are different and there is a significant difference in contrast between adjacent presentation groups (P < 0.05); the different and non-adjacent shoulder letters represent a very significant difference (P < 0.01).
In conclusion, the experiment shows that the feed additive can reduce diarrhea rate of weaned pigs, improve average daily gain, reduce death and panning rate of weaned pigs, and improve immunity of weaned pigs, and the feed additive is used in a proportion of 1:2000 added in proportion.
Comparison test:
the tests included a control group (1:2000 group), a test 1 group, a test 2 group, and a test 3 group.
The formula components of the test 1 group are 10g orange peel powder in a beaker, then 200mL of extraction liquid mixed by absolute ethyl alcohol and acetic acid (volume ratio is 9:1) is added for full dissolution, naOH is utilized to adjust the pH value to 12.0, then the compound is subjected to rotary evaporation at 45 ℃ to remove most of solvent, vacuum drying is carried out at 60 ℃, and 100-mesh crushing and sieving are utilized to obtain a product; the crushed product and the saccharicterpenin are mixed according to the weight ratio of 1:1 are used in equal amounts in combination.
The formula components of the test group 2 are 8g of chitosan oligosaccharide in a beaker, then 200mL of extraction solution mixed by absolute ethyl alcohol and acetic acid (volume ratio is 9:1) is added for full dissolution, naOH is utilized to adjust the pH value to 12.0, then the compound is subjected to rotary evaporation at 45 ℃ to remove most of solvent, vacuum drying is carried out at 60 ℃, and 100-mesh crushing and sieving are utilized to obtain a product; the crushed product and the saccharicterpenin are mixed according to the weight ratio of 1:1 are used in equal amounts in combination.
Test 3 group weighing 10g orange peel powder in a 500mL beaker, then adding 200mL of an extraction solution mixed by absolute ethyl alcohol and acetic acid (volume ratio is 9:1) for full dissolution; weighing 8g of chitosan oligosaccharide, adding 200mL of absolute ethyl alcohol and acetic acid (volume ratio is 9:1) into a 500mL beaker, and fully dissolving; then mixing the dissolved orange peel powder and chitosan oligosaccharide, regulating the pH value to 12.0 by using NaOH, removing most of the solvent by rotary evaporation of the compound at 45 ℃, drying in vacuum at 60 ℃, and then crushing and sieving by a 100-mesh sieve to obtain the product.
The mixed product of the above test group was prepared according to 1:2000 is added into the formula feed for feeding; test methods and test index measurement methods refer to the above application examples.
Table 3 statistical results of diarrhea status of weaned pigs lacking formulation components
Note that: the acronyms are identical and indicate that there is no significant difference in comparison between groups (P > 0.05); the shoulder letters are different and there is a significant difference in contrast between adjacent presentation groups (P < 0.05); the different and non-adjacent shoulder letters represent a very significant difference (P < 0.01).
As can be seen in table 3, the incidence of feed additives in trial 1 and trial 2 after addition was 33.33% and 36.67%, respectively, the average daily gain was significantly lower than that in the control group (P < 0.01) and significantly lower than that in the trial 1 and 2 groups (P < 0.05) at the lowest of the trial 3 group; the mortality test groups 1, 2 and 3 were significantly lower than the control group (P < 0.01). The invention fully shows that the diarrhea incidence and death rate of weaned pigs can be influenced by the lack of any component, wherein the average daily gain of the pigs of the test 3 group which lack the saccharicterpenin is the lowest, and the saccharicterpenin has better effect of promoting the growth of animals.
TABLE 4 influence of formula component absence on serum immunity of weaned pigs
| Group of | Control group | Test 1 group | Test 2 groups | Test 3 groups |
| IgA | 0.728±0.009 a | 0.601±0.007 b | 0.628±0.008 b | 0.611±0.009 b |
| IgG | 2.389±0.016 a | 2.152±0.004 b | 2.200±0.021 b | 2.179±0.016 b |
| IgM | 0.203±0.008 a | 0.147±0.016 a | 0.165±0.031 b | 0.156±0.008 b |
As can be seen from table 4, the IgA, igG and IgM content of the test groups 1, 2 and 3 lacking either component were significantly lower than the control group, all affecting the immunity of the piglets; any of the components of the additives of the present invention are described as indispensable.
The previous description of the embodiments is provided to facilitate a person of ordinary skill in the art in order to make and use the present invention. It will be apparent to those skilled in the art that various modifications can be readily made to these embodiments and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments, and those skilled in the art, based on the present disclosure, should make improvements and modifications without departing from the scope of the present invention.
Claims (10)
1. The preparation method of the feed additive for preventing diarrhea of weaned pigs is characterized by comprising the following steps:
s1: dissolving pericarpium Citri Tangerinae powder in extractive solution prepared by mixing anhydrous ethanol and acetic acid to obtain pericarpium Citri Tangerinae powder solution; dissolving chitosan oligosaccharide in an extracting solution formed by mixing absolute ethyl alcohol and acetic acid to obtain a chitosan oligosaccharide solution;
s2: mixing pericarpium Citri Tangerinae powder solution with chitosan oligosaccharide solution, removing solvent, pulverizing, and sieving;
s3: and (3) uniformly mixing the crushed product obtained in the step (S2) with saccharicterpenin to obtain the feed additive.
2. The method for preparing the feed additive for preventing diarrhea of weaned pigs according to claim 1, wherein in the step S1, the mass ratio of orange peel powder to chitosan oligosaccharide is 10:8, the dosage ratio of the orange peel powder to the extracting solution is 10g:200mL, the dosage ratio of chitosan oligosaccharide to the extracting solution is 8g:200mL.
3. The method for preparing the feed additive for preventing diarrhea of weaned pigs according to claim 2, wherein in the step S1, the volume ratio of the absolute ethyl alcohol to the acetic acid in the extract solution formed by mixing the absolute ethyl alcohol and the acetic acid is 9:1.
4. the method for preparing a feed additive for preventing diarrhea in weaned pigs according to claim 1, wherein in step S2, the pH is adjusted to be alkaline after mixing the orange peel powder solution with the chitosan oligosaccharide solution.
5. The method for preparing a feed additive for preventing diarrhea in weaned pigs according to claim 4, wherein NaOH is used to adjust the pH to 12.0.
6. The method for preparing a feed additive for preventing diarrhea in weaned pigs according to claim 1, wherein in step S2, the solvent is removed by means of rotary evaporation and vacuum drying.
7. The method for preparing a feed additive for preventing diarrhea in weaned pigs according to claim 1, wherein in step S2, a 100 mesh screen is used for sieving.
8. The method for preparing a feed additive for preventing diarrhea in weaned pigs according to claim 1, wherein in step S3, the pulverized product is mixed with saccharicterpenin in equal mass ratio.
9. A feed additive for preventing diarrhea in weaned pigs, characterized in that it is prepared by the method of any one of claims 1-8.
10. Use of a feed additive for preventing diarrhea in weaned pigs as claimed in claim 9 wherein the feed additive is formulated in a ratio of 1:1000-2500 mass ratio is added into feed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311210633.0A CN117064011A (en) | 2023-09-19 | 2023-09-19 | Feed additive for preventing diarrhea of weaned pigs as well as preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311210633.0A CN117064011A (en) | 2023-09-19 | 2023-09-19 | Feed additive for preventing diarrhea of weaned pigs as well as preparation method and application thereof |
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