CN117016573A - Biological bacteriostat, and preparation method and application thereof - Google Patents
Biological bacteriostat, and preparation method and application thereof Download PDFInfo
- Publication number
- CN117016573A CN117016573A CN202310832333.XA CN202310832333A CN117016573A CN 117016573 A CN117016573 A CN 117016573A CN 202310832333 A CN202310832333 A CN 202310832333A CN 117016573 A CN117016573 A CN 117016573A
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- CN
- China
- Prior art keywords
- extract
- biological
- stirring
- bacteriostat
- mixing
- Prior art date
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- Pending
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- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 claims abstract description 14
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 14
- 241000196324 Embryophyta Species 0.000 claims abstract description 11
- 239000000284 extract Substances 0.000 claims abstract description 11
- -1 N-acetylmuramyl Chemical group 0.000 claims abstract description 9
- 230000001580 bacterial effect Effects 0.000 claims abstract description 8
- FJKROLUGYXJWQN-UHFFFAOYSA-M 4-hydroxybenzoate Chemical compound OC1=CC=C(C([O-])=O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-M 0.000 claims abstract description 7
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 claims abstract description 7
- 229960000458 allantoin Drugs 0.000 claims abstract description 7
- 239000008367 deionised water Substances 0.000 claims abstract description 7
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 7
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 7
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- 239000006000 Garlic extract Substances 0.000 claims abstract description 3
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- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 36
- 238000003756 stirring Methods 0.000 claims description 26
- 238000002156 mixing Methods 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 21
- 239000000047 product Substances 0.000 claims description 14
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- 239000007788 liquid Substances 0.000 claims description 6
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- 238000007689 inspection Methods 0.000 claims description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 4
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract description 4
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- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
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- 241000194017 Streptococcus Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
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- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
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- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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Abstract
The invention discloses a biological bacteriostat, a preparation method and application thereof, and the formula is as follows: 0.1% -0.5% of parahydroxybenzoate, 0.1% -1% of allantoin, 1.5% -3.5% of N-acetylmuramyl hydrolase, 0.05% -1% of salicylic acid, 2% -5% of sage extract, 3% -7 of peppermint extract, 2% -5% of immature bitter orange extract, 1% -3% of coptis extract, 5% -10% of garlic extract, 0.5% -3% of nano silver ion and the balance of deionized water. The disinfectant is added with the components of the natural plants which are screened out, and the components which are known to be proved to be too effective in killing and inhibiting bacterial growth and reproduction, such as biotin, nano metal particles and the like, so that the disinfectant is ensured to be higher in toxicity on the basis of not losing the low pollution coefficient and small risk coefficient of the bacteriostatic agent, and the adverse reaction which is common in the disinfectant and is easy to cause skin intolerance and serious allergic burn is avoided. The biological safety performance of the method ensures that the method can play a role in various environments and application scenes.
Description
Technical Field
The invention belongs to the field of biochemistry, relates to a biological bacteriostat, a preparation method and application thereof, and in particular relates to a safe and stable biological bacteriostat preparation technology applicable to various application scenes.
Background
Bacteria are the most common species in pathogen infection, and can cause lesions in every living aspect, such as skin, respiratory tract, food, vegetation soil, etc. Common bacteria that can cause infection are staphylococcus aureus, haemolytic streptococcus, pseudomonas aeruginosa, escherichia coli, anaerobic bacteria, and the like. The traditional disinfecting article is mainly used for killing pathogenic microorganisms on a transmission medium, so that the pathogenic microorganisms can reach harmless requirements, the pathogenic microorganisms are killed outside an in-vivo environment, the transmission path of infectious diseases is cut off, and the purpose of controlling the infectious diseases is achieved. However, the conventional disinfectant products often have a lot of ignored or accepted damages in use, such as very harsh odor generation, cell mutation caused by long-term contact, skin irritation and even burn, and adverse reactions such as cough, chest distress, shortness of breath and the like after a large amount of inhalation; while more serious ones only require very small doses, by being combined with the evaporation of water vapor in the air, some carcinogens can be released and even directly lead to allergic reactions in the human body. The traditional disinfection method is easy to take effect quickly, and the aim of simple process is widely applied in the field of sanitation. Unlike traditional disinfectant, the bacteriostatic agent is not designed to kill most pathogens in the environment rapidly and effectively at one time, so that the toxicity and potential hazard are much smaller. Bacteriostats act by inhibiting bacterial growth. Bactericides destroy the bacterial cell structure, resulting in bacterial death. The antibacterial agent can kill bacteria, is widely applied to various fields of antibiosis, mildew prevention, disinfection and the like due to high safety and antibacterial effect, and is an ideal antibacterial agent for gynecological lotion, wet tissues, sanitary towels, oral supplies, hand sanitizers, melon and fruit fresh keeping and disinfection, environmental disinfection and the like. While bactericides may control or kill microorganisms, bacteria, fungi, and algae in aqueous systems. The main fields are agricultural bactericides and industrial bactericides. Therefore, the characteristics of low residue and low harm of the bacteriostat, low cleaning strength, strong efficacy of the bacteriostat, overlarge harm and easy dispersion result in two difficulties in selecting the sterilizing articles. The invention aims to provide a design thought, and on the premise of not changing low residue and low harm, the pathogen killing performance of the bacteriostat is improved as much as possible, and the application field and environment of the bacteriostat are widened.
Disclosure of Invention
Aiming at the problem that the disinfectant is easy to cause adverse reactions of intolerance and even serious allergic burn, the invention aims to provide a biological antibacterial agent.
Still another object of the present invention is: a preparation method of the biological bacteriostat is provided.
Yet another object of the present invention is: there is provided the use of the above product.
The invention aims at realizing the following scheme: a biological bacteriostat comprising the following characteristics: the formula of the components is as follows: 0.1% -0.5% of parahydroxybenzoate, 0.1% -1% of allantoin, 1.5% -3.5% of N-acetylmuramyl hydrolase, 0.05% -1% of salicylic acid, 2% -5% of sage extract, 3% -7 of peppermint extract, 2% -5% of immature bitter orange extract, 1% -3% of coptis extract, 5% -10% of garlic extract, 0.5% -3% of nano silver ion and the balance of deionized water.
The invention provides a preparation method of a biological bacteriostat, which comprises the following steps:
crude extraction process of natural plant components: cutting plant components, grinding to powder residue, soaking in ethanol with concentration of 75% or above 2-10 times volume for 2-30 min, filtering, and separating precipitate and filtrate; adding the precipitate into ethanol again, repeating the steps, setting volume gradient as appropriate, gradually increasing ethanol concentration, soaking for longer time as the volume is larger, and finally collecting all filtrate, shaking and mixing.
The treatment process of the extract comprises the following steps: adding natural plant extract into NaAC buffer solution with pH value of 5-8 of 1M, adding precooled 1M ethyl acetate, stirring, mixing well, standing at room temperature for 1 hr, centrifuging at 15000g-20000g for 10-15 min; taking the supernatant, adding ethyl acetate to ensure that the final concentration of the ethyl acetate reaches 50%, uniformly mixing, standing at 4 ℃ for 2 hours, and centrifuging at 15000g for 15-20 minutes; discarding the supernatant, adding distilled water to dissolve the precipitate, adding 1M Tris-HCl buffer solution, mixing according to the proportion of 17:3, uniformly mixing, standing at 4 ℃ for 2 hours, and centrifuging at 15000g for 10 minutes; adjusting the pH value to 5.4-7.8, and diluting by 20 times to obtain natural plant component extraction solution;
adding allantoin, nano silver ions and the balance deionized water into a stirring kettle, heating to 60 ℃, and stirring for 30-45min; cooling the solution in the stirring kettle to room temperature, sequentially adding extracts of crude extracts of herba Salvia officinalis, herba Menthae, fructus Aurantii Immaturus, coptidis rhizoma and Bulbus Allii, and mixing well; adding p-hydroxybenzoate, N-acetylmuramyl polysaccharide hydrolase and salicylic acid, slightly heating the solution to 45 ℃ and shaking at a rotating speed of 60 per minute for 1 hour to obtain the bacteriostatic agent; sampling from the bottom of the stirring tank at 15 minute intervals while stirring, observing the contrast sample and smell, packaging and sealing into small bottles when the contrast sample and smell reach the standards, keeping samples for other quality inspection projects and records, and immediately placing the sealed finished products into a cold storage at 4-10 ℃.
The invention provides an application of a biological antibacterial preparation, wherein the application forms of the preparation include, but are not limited to, gas spray, liquid flow agent, semisolid preparation and solid preparation; the gas preparation is spray and the like, the liquid flow agent is used for surface coating, the semisolid preparation is ointment, and the solid preparation is tablet.
The invention provides application of a biological antibacterial preparation in preparation of sanitary products and skin cleaning products.
The invention provides an application of a biological antibacterial agent in livestock breeding and agricultural environment cultivation.
The invention provides application of a biological antibacterial preparation in preparation of a medicament for treating skin diseases, wherein the skin infection property is bacterial infection.
The innovation and beneficial effects of the optimized special material of the invention are mainly shown in: the designed bacteriostat component design thought is specially aimed at the layer-by-layer improvement of the sterilizing effect of common bacteria such as escherichia coli, staphylococcus aureus and other pathogens, and the screened natural plant components are added, and simultaneously biotin, nano metal particles and other components which are known to be capable of effectively killing and inhibiting bacterial growth are also added, so that the sterilizing agent with higher toxicity, which is easy to cause adverse reactions of skin intolerance and serious allergic burn, is avoided on the basis of not losing the low risk coefficient of the bacteriostat, and is low in pollution coefficient, and chlorine-containing disinfectant, peroxide disinfectant, aldehyde disinfectant, alcohol disinfectant, iodine-containing disinfectant, phenol disinfectant, ethylene oxide, biguanide disinfectant, quaternary ammonium salt disinfectant and the like are avoided. The biological safety performance of the method ensures that the method can play a role in various environments and application scenes.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. The following examples will demonstrate the sterilization efficacy and low hazard residual design features of the present invention using different methods.
Example 1
A biological bacteriostat according to claim 1, prepared by the process of:
crude extraction of natural plant components: weighing 10g of sage, cutting into blocks, grinding to powder slag, adding 2 times of 75% ethanol, soaking for 30 minutes, filtering, and separating precipitate from filtrate; adding the precipitate into 75% ethanol with equal amount, repeating the steps, and finally collecting all filtrate, shaking and mixing uniformly; processing herba Menthae, fructus Aurantii Immaturus, coptidis rhizoma and Bulbus Allii one by the same method, and collecting and storing respectively;
the treatment process of the extract comprises the following steps: adding natural plant extract into NaAC buffer solution with pH value of 5.8 and 1M, adding pre-cooled 1M ethyl acetate, stirring, mixing, standing at room temperature for 1 hr, and centrifuging with 20000g for 10 min; taking the supernatant, adding ethyl acetate to ensure that the final concentration of the ethyl acetate reaches 50%, uniformly mixing, standing at 4 ℃ for 2 hours, and centrifuging at 15000g for 20 minutes; discarding the supernatant, adding distilled water to dissolve the precipitate, adding 1M Tris-HCl buffer solution, mixing according to the proportion of 17:3, uniformly mixing, standing at 4 ℃ for 2 hours, and centrifuging at 15000g for 10 minutes; adjusting the pH value to 5.4-7.8, and diluting by 20 times to obtain natural plant component extraction solution;
adding 1% of allantoin, 3% of nano silver ions and the balance of deionized water into a stirring kettle, heating to 60 ℃, and stirring for 30-45min; cooling the solution in the stirring kettle to room temperature, sequentially adding extracts of herba Salvia officinalis, herba Menthae, fructus Aurantii Immaturus, coptidis rhizoma and Bulbus Allii, and mixing well; then adding 0.5% of parahydroxybenzoate, 3.5% of N-acetylmuramyl hydrolase and 1% of salicylic acid, slightly heating the solution to 45 ℃ and shaking uniformly for 1 hour at a rotating speed of 60 per minute to obtain the bacteriostatic agent; sampling from the bottom of the stirring tank at 15 minute intervals while stirring, observing the contrast sample and smell, packaging and sealing into small bottles when the contrast sample and smell reach the standards, keeping samples for other quality inspection projects and records, and immediately placing the sealed finished products into a cold storage at 4-10 ℃.
Next, a biological bacteriostasis experiment was performed on the stock solution of the bacteriostat prepared in example 1 to verify the performance thereof.
The experimental method is based on the following inspection: GB 15979-2002 sanitary Standard for Disposable sanitary articles, appendix C dissolution antibacterial product antibacterial Property test
Detection environment: temperature: 22.3 ℃, humidity: 39% RH
Antibacterial property test: the test subjects were allowed to act for 5min and the test was repeated 3 times with one additional replicate group per group. The culture medium is trypticase soy peptone agar culture medium
The antibacterial effect of the stock solution of the biological antibacterial agent prepared in the embodiment 1 of the present invention on the selected strain is as follows in table 1:
after 3 repeated experiments of control and an additional group of replication groups, the product has obvious growth inhibition effect on bacteria in 5min, which shows that: the product has antibacterial effect, meets the requirement of GB 15979-2002 'Disposable sanitary product sanitary Standard' annex C4.2, and reaches the standard of the antibacterial rate of the 'Xiao' word size product.
Experimental method 2
The antibacterial effect of the antibacterial agent is tested by an in vitro antibacterial test method.
Experiments were divided into 7 groups, groups 1 to 6 being the bacteriostat prepared in example 1, and group 7 being a blank group. Wherein, the sterile filter paper is respectively smeared with the same amount of bacteriostat of the 1 st to 6 th groups, and is kept stand for 10 to 20min, and the 7 th group blank control group is obtained by soaking the sterile filter paper in normal saline for 10 to 20min.
Preparation of a culture medium: dissolving peptone in distilled water, heating and stirring to obtain liquid culture medium, adding a certain amount of agar into the liquid culture medium, heating and stirring to obtain solid culture medium, wherein peptone and distilled water are subjected to steam sterilization at 120deg.C.
The operation process comprises the following steps: taking a certain amount of fresh bacteria from solid culture medium with inoculating loop, adding liquid culture medium, diluting the liquid culture medium with distilled water 10 times, and diluting with Escherichia coli at a concentration of 3.2X10 6 CFU/g, dilution concentration of Staphylococcus aureus of 6.9X10 6 CFU/g, dilution concentration of white fungus is 5.5X10 6 CFU/g, respectively taking 1mL of the bacterial liquid, uniformly coating the bacterial liquid on an agar culture medium by using a smearing rod, tightly attaching sterilized filter paper with the diameter of 5mm on the culture medium, culturing a culture dish at 37 ℃ for 48 hours, taking out the filter paper to observe whether a paper piece has a bacteriostasis ring, measuring the diameter of the bacteriostasis ring by using a vernier caliper, and uniformly repeating each different strain for 3 times and setting a group of replication groups to evaluate the average bacteriostasis effect of the bacteria according to the presence or absence of the bacteriostasis ring and the diameter of the bacteriostasis ring
Referring to the sensitivity evaluation criteria of the antibacterial drug (sensitivity of more than 20mm, sensitivity of 20-10mm, and drug resistance of less than 10 mm), it is clear from Table 2 that 3 types of bacteria were basically sensitive to the stock solution of the antibacterial agent described in example 1, and were close to the sensitivity.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (7)
1. A biological bacteriostat, characterized in that: the formula of the components is as follows: 0.1% -0.5% of parahydroxybenzoate, 0.1% -1% of allantoin, 1.5% -3.5% of N-acetylmuramyl hydrolase, 0.05% -1% of salicylic acid, 2% -5% of sage extract, 3% -7 of peppermint extract, 2% -5% of immature bitter orange extract, 1% -3% of coptis extract, 5% -10% of garlic extract, 0.5% -3% of nano silver ion and the balance of deionized water.
2. A method of preparing a biological bacteriostat according to claim 1, comprising the steps of:
crude extraction process of natural plant components: cutting plant components, grinding to powder residue, soaking in ethanol with concentration of 75% or above 2-10 times volume for 2-30 min, filtering, and separating precipitate and filtrate; adding the precipitate into ethanol again, repeating the steps, setting the volume gradient as appropriate, gradually increasing the ethanol concentration, increasing the soaking time as the volume is larger, and finally collecting all filtrate, vibrating and mixing uniformly;
the treatment process of the extract comprises the following steps: adding natural plant extract into NaAC buffer solution with pH value of 5-8 of 1M, adding precooled 1M ethyl acetate, stirring, mixing well, standing at room temperature for 1 hr, centrifuging at 15000g-20000g for 10-15 min; taking the supernatant, adding ethyl acetate to ensure that the final concentration of the ethyl acetate reaches 50%, uniformly mixing, standing at 4 ℃ for 2 hours, and centrifuging at 15000g for 15-20 minutes; discarding the supernatant, adding distilled water to dissolve the precipitate, adding 1M Tris-HCl buffer solution, mixing according to the proportion of 17:3, uniformly mixing, standing at 4 ℃ for 2 hours, and centrifuging at 15000g for 10 minutes; adjusting the pH value to 5.4-7.8, and then diluting according to 20 times;
adding allantoin, nano silver ions and the balance deionized water into a stirring kettle, heating to 60 ℃, and stirring for 30-45min; cooling the solution in the stirring kettle to room temperature, sequentially adding extracts of herba Salvia officinalis, herba Menthae, fructus Aurantii Immaturus, coptidis rhizoma and Bulbus Allii, and mixing well; adding p-hydroxybenzoate, N-acetylmuramyl polysaccharide hydrolase and salicylic acid, slightly heating the solution to 45 ℃ and shaking at a rotating speed of 60 per minute for 1 hour to obtain the bacteriostatic agent; sampling from the bottom of the stirring tank at 15 minute intervals while stirring, observing the contrast sample and smell, packaging and sealing into small bottles when the contrast sample and smell reach the standards, keeping samples for other quality inspection projects and records, and immediately placing the sealed finished products into a cold storage at 4-10 ℃.
3. The method for preparing the biological bacteriostat according to claim 2, which is characterized by comprising the following steps:
crude extraction of natural plant components: weighing 10g of sage, cutting into blocks, grinding to powder slag, adding 2 times of 75% ethanol, soaking for 30 minutes, filtering, and separating precipitate from filtrate; adding the precipitate into 75% ethanol with equal amount, repeating the steps, and finally collecting all filtrate, shaking and mixing uniformly; processing herba Menthae, fructus Aurantii Immaturus, coptidis rhizoma and Bulbus Allii one by the same method, and collecting and storing respectively;
the treatment process of the extract comprises the following steps: adding natural plant extract into NaAC buffer solution with pH value of 5.8 and 1M, adding pre-cooled 1M ethyl acetate, stirring, mixing, standing at room temperature for 1 hr, and centrifuging with 20000g for 10 min; taking the supernatant, adding ethyl acetate to ensure that the final concentration of the ethyl acetate reaches 50%, uniformly mixing, standing at 4 ℃ for 2 hours, and centrifuging at 15000g for 20 minutes; discarding the supernatant, adding distilled water to dissolve the precipitate, adding 1M Tris-HCl buffer solution, mixing according to the proportion of 17:3, uniformly mixing, standing at 4 ℃ for 2 hours, and centrifuging at 15000g for 10 minutes; adjusting the pH value to 5.4-7.8, and diluting by 20 times to obtain natural plant component extraction solution;
adding 1% of allantoin, 3% of nano silver ions and the balance of deionized water into a stirring kettle, heating to 60 ℃, and stirring for 30-45min; cooling the solution in the stirring kettle to room temperature, sequentially adding extracts of herba Salvia officinalis, herba Menthae, fructus Aurantii Immaturus, coptidis rhizoma and Bulbus Allii, and mixing well; then adding 0.5% of parahydroxybenzoate, 3.5% of N-acetylmuramyl hydrolase and 1% of salicylic acid, slightly heating the solution to 45 ℃ and shaking uniformly for 1 hour at a rotating speed of 60 per minute to obtain the bacteriostatic agent; sampling from the bottom of the stirring tank at 15 minute intervals while stirring, observing the contrast sample and smell, packaging and sealing into small bottles when the contrast sample and smell reach the standards, keeping samples for other quality inspection projects and records, and immediately placing the sealed finished products into a cold storage at 4-10 ℃.
4. Use of a biological bacteriostasis according to claim 1, wherein the formulation is in the form of a gas spray, a liquid flow, a semi-solid formulation, a solid formulation; the gas preparation is spray, the liquid flow agent is used for surface coating, the semisolid preparation is ointment, and the solid preparation is tablet.
5. Use of a biological bacteriostat according to claim 1 for the preparation of hygiene and skin cleansing products.
6. Use of the biological bacteriostat of claim 1 in livestock breeding, in agricultural environment cultivation.
7. Use of a biological bacteriostat according to claim 1 for the preparation of a medicament for the treatment of dermatological disorders, said dermatological infections being of bacterial nature.
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