CN111296496A - Disinfectant for farm, preparation method and application - Google Patents
Disinfectant for farm, preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The invention relates to the technical field of disinfection for breeding, in particular to a disinfectant for breeding livestock and poultry, a preparation method of the disinfectant, and application of the disinfectant. The disinfectant for the farm provided by the invention comprises Chinese herbal medicine extracts, graphene oxide, sodium hypochlorite, a surfactant, water-soluble chitosan, biguanides, a buffering agent and water. The disinfectant with good stability, good sterilization effect, small toxic and side effects and excellent safety is obtained by combining the Chinese herbal medicine extract and the chemical disinfectant and compounding the Chinese herbal medicine extract and the chemical disinfectant. The Chinese herbal medicine extract adopted by the invention has wide sources, no pungent smell and less irritation to livestock and people. The invention also adopts the graphene oxide and the water-soluble chitosan, which can complex each component and reduce the loss of the sterilization medicine, thereby improving the sterilization effect, prolonging the sterilization time and further effectively improving the sterilization efficiency.
Description
Technical Field
The invention relates to the technical field of disinfection for breeding, in particular to a disinfectant for breeding livestock and poultry, a preparation method of the disinfectant, and application of the disinfectant.
Background
In the process of large-scale cultivation, the disinfection work is particularly important. Especially in livestock and poultry breeding industry, livestock and poultry houses, appliances and the like can attach various viruses and bacteria, especially strong infectious diseases such as avian influenza, African swine fever and the like, and the germs can cause death of a large number of livestock and poultry, can also cause reduction of animal production performance, reduce feed conversion rate, greatly increase production cost and cause huge economic loss, and infectious diseases suffered by some people and livestock also seriously threaten the body health of people. The most effective means for preventing and controlling epidemic diseases are immunization and disinfection. The effective disinfectant is selected to thoroughly kill pathogenic microorganisms and viruses, so that comprehensive and effective epidemic prevention is guaranteed.
The existing disinfectants are various, and common disinfectant products are mainly synthesized by chemical engineering and comprise chlorine-containing disinfectants, peroxide disinfectants, aldehyde disinfectants, alcohol disinfectants, ethylene oxide, polyguanidine disinfectants and the like.
However, although these chemical disinfectants have strong sterilizing power, because these chemicals have poor stability and strong pungent odor, and are easy to cause drug resistance of germs, the sterilizing effect is not ideal. The following patent documents disclose sterilizing solutions for livestock and poultry farms: CN106035404A discloses a sterilizing and disinfecting liquid for livestock and poultry and farms, which is characterized by comprising the following components in parts by weight: 6-14 parts of alkyl aryl sodium sulfonate, 4-6 parts of potassium permanganate, 5-9 parts of coconut oil fatty acid monoethanolamine, 8-10 parts of clove, 8-10 parts of rhizoma atractylodis, 8-10 parts of chrysanthemum indicum, 3-7 parts of eucalyptus leaf volatile oil, 4-8 parts of menthol, 5-9 parts of gypsum, 4-8 parts of sorbic acid, 1-3 parts of polyallyl alcohol, 2-4 parts of ethylene diamine tetraacetic acid and 80-100 parts of deionized water.
The above patent applications adopt Chinese herbal medicines as raw materials, can quickly and effectively sterilize, kill pests and disinfect livestock and poultry and dwellings thereof, has pleasant fragrance, and ensures the cleanness and the asepsis of the livestock and poultry dwellings.
However, the specific bactericidal effect, such as the bacteriostatic rate, is not disclosed in detail. Disclosure of Invention
In order to solve the problems, the invention provides the disinfectant which has the advantages of good stability, high safety, wide sources, low cost, no pollution to the environment and high virus and bacteria killing rate.
The purpose of the invention is realized as follows: a disinfectant comprises Chinese herbal medicine extract, graphene oxide, sodium hypochlorite, surfactant, water-soluble chitosan, biguanides, buffer and water.
The disinfectant comprises the following main raw materials in parts by weight: water: 80-100 parts of Chinese herbal medicine extract: 0.05-0.35 parts of graphene oxide: 0.01-0.03 part, sodium hypochlorite: 50 parts, 0.1-0.5 part of surfactant, water-soluble chitosan: 0.5-1.5 parts of biguanides: 0.1-0.3 part and 1-2 parts of buffering agent.
The Chinese herbal extract comprises at least one of Sargassum thunbergii extract and herba Houttuyniae extract. The sargassum thunbergii extract is mainly used for inhibiting the growth of tumor cells and is applied to the bacteriostasis process of agricultural production, for example, the sargassum thunbergii polyphenol can effectively control the development of gray mold of fruits, and the gray mold incidence rate and the area of disease spots of the fruits treated by the sargassum thunbergii polyphenol are obviously reduced compared with the control. There are few reports or disclosures on whether or not sargassum thunbergii extracts can be used for sterilization and disinfection in the breeding industry. The invention is creatively applied to livestock breeding, and various experiments show that the sterilization and disinfection effect is obvious after the application.
The houttuynia cordata extract has the main effects of clearing away heat and toxic materials, inducing diuresis and reducing edema, has good treatment effect on various inflammations of a human body, and has obvious inhibition effect on several common microorganisms such as staphylococcus aureus, escherichia coli, bacillus subtilis, penicillium, aspergillus niger, saccharomyces cerevisiae and the like. The literature of applying the antibacterial disinfectant to livestock and poultry breeding is disclosed. In the process of livestock and poultry breeding, certain viruses exist besides bacteria and fungi.
The graphene oxide is prepared by a Hummers oxidation method, and the surface of the graphene oxide contains abundant hydrophilic groups such as hydroxyl groups.
The surfactant is at least one of linear alkyl benzene sulfonic acid sodium (LAS), fatty alcohol-polyoxyethylene ether sodium sulfate (AES), fatty alcohol-polyoxyethylene ether ammonium sulfate (AESA), diethanolamide (6501) glycerol monostearate, lignosulfonate, heavy alkylbenzene sulfonate, alkyl sulfonate (petroleum sulfonate), alkyl polyether (PO-EO copolymer) and fatty alcohol-polyoxyethylene (3) ether (AEO-3), preferably, the surfactant is α -olefin sulfonate, the main components of α -olefin sulfonate are olefin sulfonate and hydroxysulfonate, the olefin sulfonate and hydroxysulfonate have the advantages of strong foamability, small irritation to skin, high biodegradation degree and low cost, and the disinfectant can be applied to a disinfectant used for breeding, so that the disinfectant can achieve the purposes of quick and efficient disinfection on one hand, and on the other hand, the disinfectant can effectively kill common pathogenic bacteria or viruses such as SARS, avian influenza and the like on the other hand.
The biguanide is selected from one of polymethylene biguanides, such as polyhexamethylene biguanide.
The buffer is sodium citrate.
The invention also provides a preparation method of the disinfectant, which comprises the following steps:
s1: adding water into a stirrer, then adding graphene oxide, a surfactant and a buffering agent, and starting ultrasonic to disperse so as to fully disperse the raw materials;
s2, adding the Chinese herbal medicine extract into the dispersed material obtained in the step S1, and continuously stirring at a high speed to completely dissolve the material;
s3: slowly adding sodium hypochlorite and a biguanide disinfectant into the system, and keeping the sodium hypochlorite and the biguanide disinfectant in a state of dissolving while stirring;
s4: slowly adding water-soluble chitosan into the stirred material obtained in the step S3, keeping stirring at a low speed, stopping stirring after the water-soluble chitosan is completely dissolved, standing, and obtaining a compound disinfectant after foams of the water-soluble chitosan completely disappear;
preferably, the power of the ultrasound in S1 is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
the rotation speed of high-speed stirring in the S2 is 5000-6000 r/min;
the stirring speed in S3 is 200-300 r/min;
the stirring speed in S4 is 30-40 r/min.
The invention has the beneficial effects that:
(1) the invention combines the Chinese herbal medicine extract with the chemical disinfectant, and obtains the disinfectant with good stability, good sterilization effect, small toxic and side effects and excellent safety;
(2) the Chinese herbal medicine extract adopted by the invention has wide source and no peculiar smell, and has less irritation to livestock and people, but because the Chinese herbal medicine extract is often low in water solubility, in order to improve the water solubility, the surfactant is added, so that the solubility of the Chinese herbal medicine extract is improved, active ingredients in the Chinese herbal medicine extract are more sufficiently dissolved out, and the sterilization effect of the disinfectant is improved;
(3) according to the invention, the addition of the graphene oxide and the water-soluble chitosan can complex all components, and the polyhydroxy structure of the graphene oxide and the porous macromolecular structure of the chitosan are utilized to adsorb the surface of a sprayed object, so that the loss of the sterilization medicine is reduced, the sterilization effect is improved, the sterilization time is prolonged, and the sterilization efficiency is effectively improved.
(3) In order to improve the effect of the prepared disinfectant to the maximum extent, the invention combines a preparation method of a specific disinfectant, in the method, graphene, a surfactant and a buffering agent are dispersed by ultrasonic, then a Chinese herbal medicine extract is added to dissolve the material, the third step is to add chemical disinfection components such as sodium hypochlorite and biguanide disinfectants, and finally water-soluble chitosan is added, the solubility of the material is enhanced through the stepwise addition and the full dispersion in different sequences and batches, the material can be fully dissolved out, the complexation of the material is also enhanced, each component of the system is complexed, and the stability of the prepared disinfectant is improved.
(4) The preparation method is relatively simple in preparation process, and can exert the maximum effect of each component to a great extent, so that the finally obtained product has excellent disinfection and sterilization performance and good stability.
Detailed Description
In order to further understand the present invention, the following description is given in conjunction with specific examples. The invention selects the Chinese herbal medicine extract as one of the components of the disinfectant, the Chinese herbal medicine extract comprises the sargassum thunbergii extract and the houttuynia cordata extract, and the disinfectant has the advantages of wide source, strong bactericidal capability, small side effect and small irritation to people and livestock, and is a very good disinfectant.
Example 1
Taking the preparation of the disinfectant for chicken raising factories as an example, the preparation method comprises the following steps:
s1, adding 100 parts of deionized water into a stirring container, adding 0.01 part of graphene oxide, 0.1 part of α -alkene sulfonate and 1 part of sodium citrate, and then starting ultrasound to disperse to fully disperse the graphene oxide;
the power during ultrasonic dispersion is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
s2, sequentially adding 0.01 part of sargassum thunbergii extract and 0.05 part of houttuynia cordata extract into the dispersed material in the step S1, and continuously stirring at the rotating speed of 5000-;
s3: slowly adding 50 parts of sodium hypochlorite and 0.1 part of polyhexamethylene biguanide disinfectant into the material dissolved in the step S2, and stirring and dissolving at the rotating speed of 200-300 r/min;
s4: slowly adding 0.5 part of water-soluble chitosan into S3, stirring at the rotating speed of 30-40 r/min, stopping stirring after the chitosan is completely dissolved, standing, and obtaining the compound disinfectant after foams completely disappear.
Example 2
Taking preparation of a disinfectant in a pig raising factory as an example, the preparation method comprises the following steps of S1, adding 100 parts of deionized water into a stirring container, then adding 0.02 part of graphene oxide, 0.1 part of fatty alcohol-polyoxyethylene ether sodium sulfate, 0.2 part of α -alkene sulfonate and 1.5 parts of sodium citrate, and then starting ultrasound to disperse to fully disperse the graphene oxide;
the power during ultrasonic dispersion is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
s2, sequentially adding 0.05 part of sargassum thunbergii extract and 0.2 part of houttuynia cordata extract into the dispersed material in the step S1, and continuously stirring at the rotating speed of 5000-;
s3: slowly adding 50 parts of sodium hypochlorite and 0.2 part of polyhexamethylene biguanide disinfectant into the system, and stirring and dissolving at the rotating speed of 200-300 r/min;
s4: slowly adding 1 part of water-soluble chitosan into S3, stirring at the rotating speed of 30-40 r/min, stopping stirring after the chitosan is completely dissolved, standing, and obtaining the compound disinfectant after foams of the chitosan completely disappear.
Example 3
Taking the preparation of the disinfectant for chicken raising factories as an example, the preparation method comprises the following steps:
s1, adding 90 parts of deionized water into a stirring container, adding 0.03 part of graphene oxide, 0.45 part of α -olefin sulfonate, 0.05 part of sodium fatty alcohol-polyoxyethylene ether sulfate and 2 parts of sodium citrate, and then starting ultrasound to disperse the graphene oxide fully;
the power during ultrasonic dispersion is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
s2, sequentially adding 0.1 part of sargassum thunbergii extract and 0.25 part of houttuynia cordata extract into the dispersed material in the step S1, and continuously stirring at the rotating speed of 5000-;
s3: slowly adding 50 parts of sodium hypochlorite and 0.3 part of polyhexamethylene biguanide disinfectant into the system, and stirring and dissolving at the rotating speed of 200-300 r/min;
s4: slowly adding 1.5 parts of water-soluble chitosan into S3, stirring at the rotating speed of 30-40 r/min, stopping stirring after the chitosan is completely dissolved, standing, and obtaining the compound disinfectant after foams completely disappear.
With respect to the disinfectant product of the present invention, the present inventors conducted a number of experiments to finally obtain the product of the present invention, and the present inventors expressed some of the experiments in the form of comparative examples as follows:
comparative example 1
Compared with the example 1, the comparative example 1 is the same as the example 1 except that the Chinese herbal medicine extract is not added, and the specific steps are as follows:
s1: adding 0.01 part of graphene oxide, 0.1 part of fatty alcohol-polyoxyethylene ether sodium sulfate and 1 part of sodium citrate into 100 parts of deionized water, and then starting ultrasonic to disperse so that the graphene oxide is fully dispersed;
the power during ultrasonic dispersion is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
s2: slowly adding 50 parts of sodium hypochlorite and 0.1 part of polyhexamethylene biguanide disinfectant into the dispersed material obtained in the step S1, and stirring and dissolving at the rotation speed of 200-300 r/min;
s3: and (3) slowly adding 0.5 part of water-soluble chitosan into the material dissolved in the step S2, stirring at the rotating speed of 30-40 r/min, stopping stirring after the chitosan is completely dissolved, standing, and obtaining the compound disinfectant after foams of the chitosan completely disappear.
Comparative example 2
Compared with the embodiment 1, the graphene oxide, the sodium fatty alcohol-polyoxyethylene ether sulfate and the sodium citrate are not added in the comparative example 2; the rest is the same as the embodiment 1, and the specific steps are as follows:
s1: adding 100 parts of deionized water, sequentially adding 0.01 part of Sargassum thunbergii extract and 0.05 part of herba Houttuyniae extract, and stirring at a rotation speed of 5000-;
s2: slowly adding 50 parts of sodium hypochlorite and 0.1 part of polyhexamethylene biguanide disinfectant into the system, and stirring and dissolving at the rotating speed of 200-300 r/min;
s3: and (3) slowly adding 0.5 part of water-soluble chitosan into the material dissolved in the step S2, stirring at the rotating speed of 30-40 r/min, stopping stirring after the chitosan is completely dissolved, standing, and obtaining the compound disinfectant after foams of the chitosan completely disappear.
Comparative example 3
In comparison with example 1, comparative example 3, which has the same structure as example 1, is obtained by replacing the sequence of S1 and S2:
s1: adding 100 parts of deionized water, sequentially adding 0.01 part of Sargassum thunbergii extract and 0.05 part of herba Houttuyniae extract, and stirring at a rotation speed of 5000-;
s2, adding 0.01 part of graphene oxide, 0.1 part of fatty alcohol-polyoxyethylene ether sodium sulfate and 1 part of sodium citrate into the material dissolved in the S1, and then starting ultrasound to disperse the graphene oxide fully;
the power during ultrasonic dispersion is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
s3: slowly adding 50 parts of sodium hypochlorite and 0.1 part of polyhexamethylene biguanide disinfectant into the dispersed material obtained in the step S2, and stirring and dissolving at the rotation speed of 200-300 r/min;
s4: slowly adding 0.5 part of water-soluble chitosan into S3, stirring at the rotating speed of 30-40 r/min, stopping stirring after the chitosan is completely dissolved, standing, and obtaining the compound disinfectant after foams completely disappear.
Comparative example 4
The step S3 is used as a first step, and the rest is the same as in example 1, specifically as follows:
s1: slowly adding 50 parts of sodium hypochlorite and 0.1 part of polyhexamethylene biguanide disinfectant into 100 parts of deionized water, and stirring and dissolving at the rotating speed of 200-300 r/min;
s2: adding 0.01 part of graphene oxide, 0.1 part of fatty alcohol-polyoxyethylene ether sodium sulfate and 1 part of sodium citrate into the material of S1, and then starting ultrasonic to disperse so that the graphene oxide is fully dispersed;
the power during ultrasonic dispersion is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
s3, sequentially adding 0.01 part of sargassum thunbergii extract and 0.05 part of houttuynia cordata extract into the dispersed material in the step S2, and continuously stirring at the rotating speed of 5000-;
s4: slowly adding 0.5 part of water-soluble chitosan into S3, stirring at the rotating speed of 30-40 r/min, stopping stirring after the chitosan is completely dissolved, standing, and obtaining the compound disinfectant after foams completely disappear.
Comparative example 5
The same procedures as in example 1 were repeated except that the first step was the step S3, the second step was the step S2, and the third step was the step S1:
s1: slowly adding 50 parts of sodium hypochlorite and 0.1 part of polyhexamethylene biguanide disinfectant into 100 parts of deionized water, and stirring and dissolving at the rotating speed of 200-300 r/min;
s2, sequentially adding 0.01 part of sargassum thunbergii extract and 0.05 part of houttuynia cordata extract into the material dissolved in the step S1, and continuously stirring at the rotating speed of 5000-;
s3: adding 0.01 part of graphene oxide, 0.1 part of fatty alcohol-polyoxyethylene ether sodium sulfate and 1 part of sodium citrate in the step S2, and then starting ultrasound to disperse so that the graphene oxide is fully dispersed;
the power during ultrasonic dispersion is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
s4: and (3) slowly adding 0.5 part of water-soluble chitosan into the material dissolved in the step S3, stirring at the rotating speed of 30-40 r/min, stopping stirring after the chitosan is completely dissolved, standing, and obtaining the compound disinfectant after foams of the chitosan completely disappear.
Comparative example 6
The step S4 is used as the first step, and the rest is the same as the example 1, and the specific steps are as follows:
s1: slowly adding 0.5 part of water-soluble chitosan into 100 parts of deionized water, stirring at a rotating speed of 30-40 r/min, and stopping stirring after the chitosan is completely dissolved;
s2: adding 0.01 part of graphene oxide, 0.1 part of fatty alcohol-polyoxyethylene ether sodium sulfate and 1 part of sodium citrate into the material dissolved in the step S1, and then starting ultrasonic to disperse so that the graphene oxide is fully dispersed;
the power during ultrasonic dispersion is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
s3, sequentially adding 0.01 part of sargassum thunbergii extract and 0.05 part of houttuynia cordata extract into the dispersed material in the step S2, and continuously stirring at the rotating speed of 5000-;
s4: slowly adding 50 parts of sodium hypochlorite and 0.1 part of polyhexamethylene biguanide disinfectant into the material dissolved in the step S2, and stirring and dissolving at the rotating speed of 200-300 r/min; after being dissolved, the compound disinfectant is obtained.
Comparative example 7
In comparative example 7, the houttuynia cordata extract was replaced with the dandelion extract, and the procedure was the same as in example 1, and the specific steps were as follows:
s1: adding 100 parts of deionized water into a stirring container, adding 0.01 part of graphene oxide, 0.1 part of fatty alcohol-polyoxyethylene ether sodium sulfate and 1 part of sodium citrate, and then starting ultrasonic to disperse so that the graphene oxide is fully dispersed;
the power during ultrasonic dispersion is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
s2, sequentially adding 0.01 part of sargassum thunbergii extract and 0.05 part of dandelion extract into the dispersed material in the step S1, and continuously stirring at the rotating speed of 5000-;
s3: slowly adding 50 parts of sodium hypochlorite and 0.1 part of polyhexamethylene biguanide disinfectant into the material dissolved in the step S2, and stirring and dissolving at the rotating speed of 200-300 r/min;
s4: slowly adding 0.5 part of water-soluble chitosan into S3, stirring at the rotating speed of 30-40 r/min, stopping stirring after the chitosan is completely dissolved, standing, and obtaining the compound disinfectant after foams completely disappear.
Comparative example 8
Compared with the example 1, only the step S3 is carried out, namely the compounded disinfectant is obtained.
Comparative example 9
Compared with the example 1, only the material in the S3 is added into the deionized water, and the specific steps are as follows:
s3: slowly adding 50 parts of sodium hypochlorite and 0.1 part of polyhexamethylene biguanide disinfectant into 100 parts of deionized water, and stirring and dissolving at the rotating speed of 200-300 r/min; obtaining the compound disinfectant.
Example 4
1. And (3) testing the disinfection and sterilization effect:
the specific comparison between example 1 and the comparative examples applied to the chicken farm is as follows:
the disinfection method comprises the following steps: spraying the disinfectant according to the dosage of 200 ml per cubic meter; or fumigating with 50 ml of water per cubic meter; after sterilization the air in the chicken house was sampled. The sampling method comprises the following steps: the type of the sampler: TYK-6 type six-level sieve mesh impact type air microorganism sampler; sampling air in different henhouses of the same chicken factory;
the sampling method is that sampling points are arranged at four corners and the center of the henhouse within 1 hour before and after the sterilization, and prepared culture mediums are placed in discs of the sampler one by one. Placing the TYK-6 type six-level sieve mesh impact type air microorganism sampler on a bracket, connecting with a host, starting sampling, wherein each sampling is carried out for 5 minutes, the gas flow is 28L/min, and the sampling amount is 141L; after sampling, the TYK-6 type six-level sieve mesh impact type air microorganism sampler is opened, and the sample is taken out and cultured for 24 hours at 37 ℃ for later use. In the sampling process, when the plate for holding the sample is placed and taken out, the plate is flatly held as much as possible and is quickly placed and taken out, so that the plate is prevented from being infected by the mixed bacteria in the air.
For the culture of the collected bacteria, different bacterial colonies appear after the sampled flat plate is cultured for 24 hours, the escherichia coli presents red bacterial colonies on a culture medium of the escherichia coli, the bacterial colonies with uniform red sizes are selected for microscopic examination, and the escherichia coli is cultured for 24 hours after primary identification; single colonies were picked and inoculated onto slant medium for 24 hours of pure culture. Recording the number of colonies in the culture dish, and substituting the number into the following formula to calculate the total number of bacteria and the number of escherichia coli in the market respectively;
microbial count cfu/m3Either [ colony count on all plates/(sampling time X28L/min)]×1000
The Escherichia coli culture medium is purchased from Shanghai Bohu Biotechnology Co., Ltd;
the manufacturer of the staphylococcus aureus culture medium: qingdao Haibo Biotech limited;
the sterilization rate of escherichia coli and staphylococcus aureus can be calculated by the ratio of the number of bacteria before and after sterilization, and is specifically shown in the following table 1:
TABLE 1 inhibition table of disinfectant against pathogenic microorganism in examples and comparative examples (%)
| Escherichia coli% | Staphylococcus aureus | Salmonella | |
| Example 1 | 99.8 | 99.4 | Not detected out |
| Example 2 | 99.6 | 99.5 | Not detected out |
| Example 3 | 99.3 | 99.2 | Not detected out |
| Comparative example 1 | 78.5 | 81.3 | Not detected out |
| Comparative example 2 | 83.4 | 84.7 | Not detected out |
| Comparative example 3 | 84.2 | 83.8 | Not detected out |
| Comparative example 4 | 82.6 | 87.4 | Not detected out |
| Comparative example 5 | 85.3 | 82.1 | Not detected out |
| Comparative example 6 | 83.6 | 84.8 | Not detected out |
| Comparative example 7 | 89.5 | 91.3 | Not detected out |
| Comparative example 8 | 91.4 | 92.7 | Not detected out |
| Comparative example 9 | 66.8 | 69.3 | Not detected out |
As can be seen from the data in Table 1 above, the disinfectant in the examples of the present invention has a significant inhibition rate against Escherichia coli and Staphylococcus aureus, while the disinfectant in each comparative example has a certain disinfecting effect, but the inhibition rate against the above two strains is much lower than that in the examples of the present invention.
2. Product odor testing
After the bottle cap of the disinfectant prepared in the embodiment is opened, the nose is slightly close to the bottle mouth, the disinfectant has slight Chinese herbal medicine smell and has no obvious irritation, and the disinfectant can obviously smell the irritation smell in the comparative example.
TABLE 2 product odor of examples and comparative examples
| Smell(s) | |
| Practice ofExample 1 | Slight disinfectant taste, no pungent nasal taste |
| Example 2 | Slight disinfectant taste, no pungent nasal taste |
| Example 3 | Slight disinfectant taste, no pungent nasal taste |
| Comparative example 1 | Slight disinfectant taste, no pungent nasal taste |
| Comparative example 2 | Slight disinfectant taste, no pungent nasal taste |
| Comparative example 3 | The disinfectant has a slight pungent taste |
| Comparative example 4 | The disinfectant has a slight pungent taste |
| Comparative example 5 | The disinfectant has a slight pungent taste |
| Comparative example 6 | The disinfectant has a slight pungent taste |
| Comparative example 7 | Slight disinfectant taste, almost no pungent nasal taste |
| Comparative example 8 | The disinfectant has strong smell and pungent taste |
| Comparative example 9 | Pungent disinfectant taste |
From the above odor test results, examples 1 to 3 and comparative examples 1, 2 and 7 had good odor, only had the taste of the disinfectant, and had no pungent odor or almost no pungent odor; comparative examples 3 to 6 had a slightly pungent taste; comparative example 8 was pungent in odor, and comparative example 9 was pungent in odor and had a stronger disinfectant taste. This shows that the product of the invention has better fusion of all components, easily accepted smell, and only has the taste of common disinfectant fluid, no pungent smell or light smell.
3. Stability test
Regarding the detection of product stability, the judgment of appearance is represented by "+" and "-", and the details are as follows:
+ + + + indicates turbidity of the disinfectant, with obvious sediment in the container;
+ indicates that the disinfectant is slightly turbid and there is a small amount of sediment in the container;
+ means that the disinfectant is opaque or not clear and there is a slight deposit in the container;
-indicating that the disinfectant is relatively clear, slightly precipitated, and slightly turbid on shaking;
-means that the disinfectant is transparent, free of any agglutinated precipitate, and free of uniform turbidity upon shaking;
more + indicates less product stability; more indicates better product stability. The products of the examples and comparative examples were stored at different times and the results are given in the following table:
TABLE 3 stability of the products of the examples and comparative examples at different storage times
| 1 month | 3 months old | 6 months old | 12 months old | |
| Example 1 | -- | -- | -- | -- |
| Example 2 | -- | -- | -- | -- |
| Example 3 | -- | -- | -- | -- |
| Comparative example 1 | -- | -- | -- | -- |
| Comparative example 2 | ++ | ++ | ++ | +++ |
| Comparative example 3 | + | + | + | + |
| Comparative example 4 | + | + | + | ++ |
| Comparative example 5 | ++ | ++ | ++ | ++ |
| Comparative example 6 | + | + | + | ++ |
| Comparative example 7 | -- | -- | -- | -- |
| Comparative example 8 | ++ | ++ | ++ | ++ |
| Comparative example 9 | +++ | +++ | +++ | ++++ |
As can be seen from the table, the products of examples 1 to 3 were excellent in stability, and after 12 months of shelf life, the products were still transparent and no turbidity occurred upon shaking.
In comparative example 1, no herbal extract was added; and in the comparative example 7, the Chinese herbal medicine extract is replaced; the result shows that the appearance transparency of the two is good, even after 12 months of storage, the two still have almost no sediment, and the appearance is transparent; this indicates that the Chinese herbal medicine has less influence on the stability of the product;
in comparative example 2, graphene oxide and sodium fatty alcohol polyoxyethylene ether sulfate were not used, and as a result, the stability was poor, which is manifested by turbidity in appearance and precipitation; this demonstrates that graphene oxide and surfactants have a significant effect on improving product stability;
in comparative examples 3, 4, 5 and 6, the feeding sequence of the raw materials is changed, so that the stability of the product is obviously influenced, and the stability of the disinfectants in the comparative examples 3 to 6 is not ideal;
the product of comparative example 8, which does not contain chitosan, shows that the stability is not ideal, because the chitosan has complexation property, and can play a role of sufficient complexation for the whole system, so as to improve the stability of the disinfectant.
In comparative example 9, the stability is the worst, which indicates that the graphene oxide, the surfactant sodium alcohol ether sulfate and the chitosan adopted in the invention have a great influence on improving the product stability.
The invention adds sodium hypochlorite and biguanide disinfectants on the basis of the Chinese herbal medicine extract, and the chemical disinfectants have the characteristics of strong sterilizing capability, no secondary pollution, difficult generation of drug resistance to pathogenic bacteria and broad-spectrum application.
The invention also adopts the graphene oxide and the water-soluble chitosan, the graphene oxide has good sterilization effect on one hand, and has the characteristics of large surface area and strong adsorption capacity on the other hand, the graphene oxide can play a role in complexing sterilization components in the disinfectant, and can play a role in adsorbing a base material in the spraying process, so that the sterilization effect is improved, and the water-soluble chitosan also has the characteristic of strong adsorption capacity, so that the sterilization effect of the bactericide can be greatly improved.
4. And (3) irritation test:
in order to verify the irritation of the product of the invention, the inventor conducts verification through a mouse test, and the specific method is as follows:
adult healthy mice are selected, the male and female parts of the mice are respectively 40, the weight of the mice is about 150, the mice are randomly divided into a complete skin group and a damaged skin group by adopting the self comparison of the same body, the female and male parts of each group are respectively cut off hairs on two sides of a vertebral column of the mice 24 hours before the test, each side is 4cm multiplied by 5cm, the damaged skin is prepared by removing hairs of the mice, disinfecting the skins (hair removal positions) on two sides, cutting the skins with a disinfection operation needle as a 'well' word, the length of a cut is about 2cm, and the damage degrees of the skins on the left side and the right side are basically consistent by taking blood seepage as a degree. The left side was backed with the disinfectant of example 1 of the present invention, and the right side was backed with vehicle (50% by volume ethanol), and each mouse was housed in a cage. The drugs and excipients were washed with warm water 24 hours after application, and the presence or absence of erythema and edema at the application site was continuously observed and recorded at 1 hour, 24 hours, 48 hours and 72 hours, respectively.
Grading standard: according to the guiding principle of preclinical research of new traditional Chinese medicines, the degree of erythema and edema is set to 0-4 groups and irritation evaluation is carried out, and the equal division values of erythema-free, barely visible erythema, obvious visibility, moderate to severe erythema and purplish red erythema with eschar formation are respectively marked as 0, 1, 2, 3 and 4, the equal division values of edema-free, barely visible, visibility (the edge is higher than the surrounding skin), skin uplift is about 1mm, the uplift is clear, the edema uplift is more than 1mm, and the division values of the range expansion are respectively marked as 0, 1, 2, 3 and 4.
The specific score table is as follows:
TABLE 4 reaction of mice with erythema or edema at different time points
The results show that no irritation reaction such as erythema and edema was observed on the intact skin and the injured skin of the mice 48 hours after the single-application irritation, and erythema was observed after 24 hours in the intact skin group and the injured skin group, but the average irritation reaction scores were 0 after 48 hours. This indicates that the disinfectant of the present invention is non-irritating or less irritating.
Through the above experimental tests, the product of the invention has the following characteristics:
1, the sterilization effect is good; the inhibition rate of the bacillus coli and the staphylococcus aureus can reach about 99 percent; 2, the product has good stability, and after 12 months of storage, the product still has stable performances, transparent appearance and almost no precipitate; 3, the smell is not pungent and is easy to be accepted; 4 is less or substantially non-irritating to the skin.
The disinfectant disclosed by the invention adopts plant raw materials such as Chinese herbal medicine extracts, graphene oxide and water-soluble chitosan which enhance the sterilization effect and stability, and α -olefin sulfonate as a foaming agent, so that the contact chance of molecules of a disinfection substance and toxic molecules is enhanced, and the solubility of the disinfectant in the disinfectant is improved.
Claims (10)
1. The disinfectant for the farm is characterized in that main raw materials of the disinfectant comprise Chinese herbal medicine extracts, graphene oxide, sodium hypochlorite, a surfactant, water-soluble chitosan, biguanides, a buffering agent and water.
2. The disinfectant for the farm according to claim 1, wherein the disinfectant comprises the following raw materials in parts by weight: water: 80-100 parts of Chinese herbal medicine extract: 0.05-0.35 parts of graphene oxide: 0.01-0.03 part, sodium hypochlorite: 50 parts, 0.1-0.5 part of surfactant, water-soluble chitosan: 0.5-1.5 parts of biguanides: 0.1-0.3 part and 1-2 parts of buffering agent.
3. The disinfectant for farm use according to claim 1, wherein said herbal extract is at least one of a sargassum thunbergii extract and a houttuynia cordata extract.
4. A disinfectant for farm use according to claim 1, wherein said surfactant is selected from at least one of linear alkyl benzene sulfonic acid sodium, fatty alcohol-polyoxyethylene ether sodium sulfate, fatty alcohol-polyoxyethylene ether ammonium sulfate, diethanol amide stearic acid monoglyceride, lignosulfonate, heavy alkylbenzene sulfonate, alkyl polyether, fatty alcohol-polyoxyethylene ether;
preferably, the water is deionized water;
preferably, the surfactant is α -olefin sulfonate.
5. A disinfectant for farm use according to claim 1, wherein said biguanide is selected from the group consisting of polymethylene biguanides.
6. A disinfectant for farm use according to claim 1, wherein said biguanide is polyhexamethylene biguanide; preferably, the buffer is sodium citrate.
7. The method for preparing a disinfectant for a farm according to claim 1, comprising: the method comprises the following steps:
s1: adding water into a stirrer, then adding graphene oxide, a surfactant and a buffering agent, and starting ultrasonic to disperse so that the raw materials are fully dispersed:
s2, adding the Chinese herbal medicine extract into the dispersed material obtained in the step S1, and continuously stirring at a high speed to completely dissolve the material;
s3: slowly adding sodium hypochlorite and a biguanide disinfectant into the system, and keeping the state of dissolving while stirring:
s4: slowly adding water-soluble chitosan into the stirred material obtained in the step S3, keeping stirring at a low speed, stopping stirring after the water-soluble chitosan is completely dissolved, standing, and obtaining a compound disinfectant after foams of the water-soluble chitosan completely disappear;
preferably, the power of the ultrasound in S1 is 100W, and the frequency is 50 KHz; the ultrasonic treatment time is 2-4 h;
the rotation speed of high-speed stirring in the S2 is 5000-6000 r/min;
the stirring speed in S3 is 200-300 r/min; the stirring speed in S4 is 30-40 r/min.
8. A disinfectant for farm use according to claims 1-6, wherein said disinfectant further comprises at least one of a bactericide and an adsorbent.
9. Use of a disinfectant for farm use according to claims 1-6 in live pig farming.
10. The use of the disinfectant for farms according to claims 1 to 6 in the cultivation of laying hens and broilers.
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| CN112369422A (en) * | 2020-11-16 | 2021-02-19 | 兰州理工大学 | Graphene oxide-trichloroisocyanuric acid compound, preparation method and application |
| CN113559120A (en) * | 2021-07-30 | 2021-10-29 | 桂林清研皓隆新材料有限公司 | Newborn umbilical region care solution and preparation method thereof |
| CN113841636A (en) * | 2021-08-24 | 2021-12-28 | 中国水产科学研究院珠江水产研究所 | Mandarin fish virus-free seedling breeding method |
| WO2023030374A1 (en) * | 2021-09-03 | 2023-03-09 | 中国人民解放军军事科学院军事医学研究院 | Decontamination composition and application thereof |
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| WO2023030374A1 (en) * | 2021-09-03 | 2023-03-09 | 中国人民解放军军事科学院军事医学研究院 | Decontamination composition and application thereof |
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Address after: No. 301-11, No. 1006, Shunfeng Road, hi tech Zone, Jinan City, Shandong Province Applicant after: Shandong huawodeyuan Biotechnology Co.,Ltd. Address before: 250101 411, floor 4, building 1, mass entrepreneurship and innovation base, north section of Gangxing Third Road, high tech Zone, Jinan City, Shandong Province Applicant before: Shandong Jiuhe Weisheng Biotechnology Co.,Ltd. |
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