CN117016557A - Bactericide composition for preventing and treating agaricus bisporus diseases and application thereof - Google Patents
Bactericide composition for preventing and treating agaricus bisporus diseases and application thereof Download PDFInfo
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- CN117016557A CN117016557A CN202310722496.2A CN202310722496A CN117016557A CN 117016557 A CN117016557 A CN 117016557A CN 202310722496 A CN202310722496 A CN 202310722496A CN 117016557 A CN117016557 A CN 117016557A
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- agaricus bisporus
- manganese chloride
- octreotide acetate
- diseases
- arachnoid
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- 201000010099 disease Diseases 0.000 title claims abstract description 58
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 58
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 43
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 41
- 239000000203 mixture Substances 0.000 title claims abstract description 22
- 241000222519 Agaricus bisporus Species 0.000 title claims abstract 21
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims abstract description 48
- 229940099607 manganese chloride Drugs 0.000 claims abstract description 48
- 239000011565 manganese chloride Substances 0.000 claims abstract description 48
- OXOKTPUZOHPXSA-UHFFFAOYSA-L manganese(2+);n-propyl-n-[2-(2,4,6-trichlorophenoxy)ethyl]imidazole-1-carboxamide;dichloride Chemical compound Cl[Mn]Cl.C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl.C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl.C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl.C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl OXOKTPUZOHPXSA-UHFFFAOYSA-L 0.000 claims abstract description 48
- 235000002867 manganese chloride Nutrition 0.000 claims abstract description 43
- 108010016076 Octreotide Proteins 0.000 claims abstract description 42
- 229960001494 octreotide acetate Drugs 0.000 claims abstract description 42
- DEQANNDTNATYII-OULOTJBUSA-N (4r,7s,10s,13r,16s,19r)-10-(4-aminobutyl)-19-[[(2r)-2-amino-3-phenylpropanoyl]amino]-16-benzyl-n-[(2r,3r)-1,3-dihydroxybutan-2-yl]-7-[(1r)-1-hydroxyethyl]-13-(1h-indol-3-ylmethyl)-6,9,12,15,18-pentaoxo-1,2-dithia-5,8,11,14,17-pentazacycloicosane-4-carboxa Chemical compound C([C@@H](N)C(=O)N[C@H]1CSSC[C@H](NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](CC=2C3=CC=CC=C3NC=2)NC(=O)[C@H](CC=2C=CC=CC=2)NC1=O)C(=O)N[C@H](CO)[C@H](O)C)C1=CC=CC=C1 DEQANNDTNATYII-OULOTJBUSA-N 0.000 claims abstract description 41
- 239000000575 pesticide Substances 0.000 claims abstract description 23
- 239000004480 active ingredient Substances 0.000 claims abstract description 10
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 10
- 244000052769 pathogen Species 0.000 claims abstract description 8
- 241000191967 Staphylococcus aureus Species 0.000 claims abstract description 7
- 210000000576 arachnoid Anatomy 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 230000000855 fungicidal effect Effects 0.000 claims 2
- 239000000417 fungicide Substances 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 abstract description 28
- 238000011282 treatment Methods 0.000 abstract description 27
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- 238000011161 development Methods 0.000 abstract description 5
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- 206010059866 Drug resistance Diseases 0.000 abstract description 4
- 231100000419 toxicity Toxicity 0.000 abstract description 4
- 230000001988 toxicity Effects 0.000 abstract description 4
- 208000031888 Mycoses Diseases 0.000 abstract description 2
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 230000003111 delayed effect Effects 0.000 abstract 1
- 230000006806 disease prevention Effects 0.000 abstract 1
- 244000251953 Agaricus brunnescens Species 0.000 description 75
- 230000000694 effects Effects 0.000 description 19
- 238000012360 testing method Methods 0.000 description 17
- 239000003795 chemical substances by application Substances 0.000 description 7
- 230000002265 prevention Effects 0.000 description 7
- 230000002195 synergetic effect Effects 0.000 description 7
- 241000221931 Hypomyces rosellus Species 0.000 description 6
- 239000004563 wettable powder Substances 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- NSJANQIGFSGFFN-UHFFFAOYSA-N octylazanium;acetate Chemical compound CC([O-])=O.CCCCCCCC[NH3+] NSJANQIGFSGFFN-UHFFFAOYSA-N 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000239290 Araneae Species 0.000 description 4
- 239000000945 filler Substances 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- -1 prochloraz-manganese chloride salt Chemical class 0.000 description 4
- 241000554002 Cladobotryum mycophilum Species 0.000 description 3
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- 239000012752 auxiliary agent Substances 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
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- 235000019750 Crude protein Nutrition 0.000 description 2
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- 231100000989 no adverse effect Toxicity 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
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- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000375 suspending agent Substances 0.000 description 2
- 239000004562 water dispersible granule Substances 0.000 description 2
- 239000000080 wetting agent Substances 0.000 description 2
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241001057326 Auricularia heimuer Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001537207 Flammulina Species 0.000 description 1
- 240000003915 Lophatherum gracile Species 0.000 description 1
- 206010028851 Necrosis Diseases 0.000 description 1
- 241000235400 Phycomyces Species 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 235000007685 Pleurotus columbinus Nutrition 0.000 description 1
- 241001533598 Septoria Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
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- 238000003556 assay Methods 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
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- 238000011156 evaluation Methods 0.000 description 1
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- 231100000086 high toxicity Toxicity 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a bactericide composition for preventing and treating agaricus bisporus diseases and application thereof, and belongs to the technical field of edible fungus disease prevention and treatment. The bactericide composition for preventing and treating agaricus bisporus diseases comprises the active ingredients of prochloraz manganese chloride and octreotide acetate, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1-40:1. The pathogen aimed by the composition is the staphylococcus aureus, the sterilizing toxicity can be obviously improved, the synergy and the drug resistance can be delayed under the compound proportion defined by the invention, the growth safety of the agaricus bisporus is ensured, the chemical pesticide dosage is effectively reduced, and a new idea for the development and the scientific use of the medicament is opened up.
Description
Technical Field
The invention belongs to the technical field of edible fungus disease control, and particularly relates to a bactericide composition for controlling agaricus bisporus diseases and application thereof.
Background
Currently, edible fungi that can be artificially cultivated include more than 60 kinds of mushrooms (Lentinus edodes), black fungus (Auricularia heimuer), oyster mushroom (Pleurotus ostreatus), needle mushroom (Flammulina filiformis), agaricus bisporus (Agaricus bisporus), and the like. On the day of the development and steaming of the agaricus bisporus planting industry, diseases on agaricus bisporus (A.bisporus) are more common, the yield is generally reduced by 20% -30% when the diseases occur, and the yield can reach about 50% if the conditions are serious, and even is out of order when the conditions are serious. One of the serious diseases which is difficult to control is a new disease of agaricus bisporus cobweb disease.
The agaricus bisporus arachnoid disease is a new disease caused by the staphylococcus aureus (Cladobotryum mycophilum), and besides the agaricus bisporus, a plurality of edible fungi such as lentinus edodes (L.edodes) can be infected. The downy mycelium generated at the early stage of disease adheres to the soil-covering layer and gradually diffuses from the junction of the soil-covering layer and the fungus handle to the upper part of the fruiting body. The pathogenic bacteria mycelium gradually becomes cotton-like, and a large amount of spores are produced simultaneously, brown spots appear on the fruiting body of the pathogenic agaricus bisporus, and finally the fruiting body is rotted, so that the whole fruiting body can be withered when serious, and the pathogenic agaricus bisporus can be infected in the whole development period of the agaricus bisporus.
At present, the occurrence of the agaricus bisporus arachnoid disease is more serious, but few medicaments are available in the actual production of the agaricus bisporus, mainly because most medicaments inhibit the growth of pathogenic bacteria hyphae and inhibit the growth of the agaricus bisporus hyphae at the same time, thereby adversely affecting the yield and quality of the agaricus bisporus. In addition, when the agaricus bisporus arachnoid disease is prevented and treated by adopting a chemical agent prevention and treatment measure, the single agent has large dosage, is easy to generate drug resistance and has the problem of exceeding the standard of pesticide residues in order to achieve the prevention and treatment effect. Therefore, development of efficient medicaments for preventing and treating agaricus bisporus spider web diseases is needed in production. To relieve the occurrence of drug resistance of single medicament, reduce pesticide residue in agaricus bisporus and ensure the yield and quality of agaricus bisporus.
Disclosure of Invention
Aiming at the problem of lack of an effective and safe medicament for preventing and treating agaricus bisporus arachnoid disease, the invention provides a bactericide composition containing prochloraz manganese chloride and octylamine acetate for preventing and treating agaricus bisporus arachnoid disease, so as to improve the prevention and treatment effect on agaricus bisporus arachnoid disease, delay the resistance of pathogenic bacteria to imidazole bactericides and reduce the prevention and treatment cost.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the bactericide composition for preventing and treating agaricus bisporus diseases comprises prochloraz manganese chloride and octreotide acetate as effective components, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1-40:1.
Based on the scheme, the weight ratio of prochloraz manganese chloride to octreotide acetate is preferably 20:1-30:1, and more preferably 20:1.
The application of the bactericidal composition in preparing pesticides for preventing and controlling agaricus bisporus diseases, wherein the agaricus bisporus diseases are agaricus bisporus arachnoid diseases and/or agaricus bisporus brown rot diseases.
A pesticide for preventing and treating agaricus bisporus arachnoid comprises prochloraz manganese chloride and octreotide acetate, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1-40:1; preferably, the weight ratio of prochloraz-manganese chloride complex to octreotide acetate is 20:1-30:1, and more preferably 20:1.
In the pesticide for preventing and treating the agaricus bisporus cobweb disease, the content of prochloraz manganese chloride and octreotide acetate accounts for 1-80% of the total mass of the pesticide, and is preferably 10.25-52.5%.
The pesticide for preventing and treating the agaricus bisporus arachnoid disease also comprises auxiliary agents which are used for facilitating the use of the active ingredients of prochloraz manganese chloride and octreotide acetate or are uniformly distributed on crops, such as inert fillers, wetting dispersing agents, penetrating agents, emulsifying agents, solvents, antifreezing agents, defoaming agents, warning colors and the like, wherein the auxiliary agents can be solid and liquid additives which are conventionally used in the formula in the prior art.
In the presence of the conventional auxiliary agent, the pesticide can be prepared into one of water aqua, wettable powder, water dispersible granule, suspending agent and emulsion in water.
The pesticide is applied to preventing and treating the agaricus bisporus arachnoid disease caused by the agaricus bisporus arachnoid disease pathogen, wherein the agaricus bisporus arachnoid disease pathogen is staphylococcus aureus.
A method for preventing and treating agaricus bisporus spider web disease comprises spraying agaricus bisporus with pesticide for preventing and treating agaricus bisporus spider web disease; the pesticide for preventing and treating the agaricus bisporus arachnoid comprises the active ingredients of prochloraz manganese chloride and octreotide acetate, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1.
The technical scheme of the invention has the advantages that:
the invention provides a composition for preventing and controlling double-spore mushroom diseases by mixing an environment-friendly bactericide, namely, octylamine acetate and a chemical bactericide, namely, prochloraz-manganese chloride, wherein the pathogen aimed by the composition is lophatherum gracile, and the composition can obviously improve sterilizing toxicity, increase efficiency and delay drug resistance under the compound proportion defined by the invention, is safe for the growth of double-spore mushrooms, effectively reduces the dosage of chemical pesticides, and opens up a new idea for the development and scientific use of medicaments.
Detailed Description
The terms used in the present invention generally have meanings commonly understood by those of ordinary skill in the art unless otherwise indicated.
The invention will be described in further detail below in connection with specific embodiments and with reference to the data. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.
In the following examples, the two spore mushroom arachnoid pathogen used was a fungus septoria (c.mycophilum) (First Report of Cladobotryum mycophilum Causing Cobweb Disease on Agaricus bisporus in china.plant disease,2016,2334) deposited by the department of agriculture, taan city; agaricus bisporus (a.bisporus): s2796, W192, W2000, taian national academy of agricultural sciences.
The adopted medicaments are as follows: prochloraz manganese chloride salt is purchased from Shandong Runfeng chemical industry Co., ltd, and octreotide acetate is purchased from Weifang Wansheng biological pesticide Co., ltd. 50% prochloraz manganese chloride complex wettable powder is purchased from Shanxi Mebang pharmaceutical industry group Co., ltd, and 1.8% octreotide acetate aqueous solution is purchased from Shanxi Seisaku Jiake agricultural chemical Co., ltd.
The virulence regression equation and EC of the medicament to the staphylococcus aureus are calculated by adopting SPSS 25.0 software 50 Values and 95% confidence limits. Safety test and field efficacy test Ducan's method was used to compare the significance of differences between different treatments in each test.
Example 1
The bactericide composition for preventing and treating agaricus bisporus diseases comprises prochloraz manganese chloride and octreotide acetate as effective components, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1.
Example 2
The bactericide composition for preventing and treating agaricus bisporus diseases comprises prochloraz manganese chloride and octreotide acetate as effective components, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 30:1.
Example 3
The bactericide composition for preventing and treating agaricus bisporus diseases comprises prochloraz manganese chloride and octreotide acetate as effective components, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 40:1.
Example 4
A pesticide for preventing and treating agaricus bisporus cobweb disease contains 52.5% prochloraz manganese chloride + octylamine acetate (20:1) as effective component.
The raw materials comprise the following components in parts by weight: 50 parts of prochloraz manganese chloride, 2.5 parts of octreotide acetate, 7 parts of wetting agent and 100 parts of filler.
The preparation method comprises the following steps: the prochloraz manganese chloride, the octreotide acetate, the wetting agent and the filler (any one of white carbon black, kaolin and diatomite) are weighed, active ingredients are firstly adsorbed by the filler, uniformly mixed, and then the mixture is subjected to air current crushing to prepare the wettable powder with the active ingredient content of 52.5%.
The wettable powder can be prepared into water dispersible granules through granulation.
The wettable powder can be processed into a suspending agent by adding water and grinding through sand grinding.
Influence of the Compound bactericide on the growth of the mycelial of the Phycomyces philic and the mycelial of the Agaricus bisporus
The compound bactericide adopts three combinations of prochloraz manganese chloride and octreotide acetate with the weight ratio of 20:1, 30:1 and 40:1. In the plate assay, EC according to single agents 50 Designing the dosing concentration range of the compound bactericide, and designing the test concentration range of the compound bactericide for influencing the growth of the agaricus bisporus arachnoid hyphae to be 0.25-8mg.L -1 The mass concentration of the two single medicines is consistent with that of the compound medicine.
Test concentration design selection agent for influence of compound bactericide on growth of agaricus bisporus mycelium and EC of agaricus bisporus arachnoid pathogen 50 Value, 2 times EC 50 Value and 4 times EC 50 The values were 3 treatment concentrations.
EC of compound bactericide 50 Theoretical value (X) 1 ) And the synergy coefficient (synergistic ratio, SR) are calculated as follows:
X 1 ＝(P A +P B )/(P A /A+P B /B) (1)
wherein P is A The composition is the percentage of single medicine A in the compound bactericide; p (P) B The composition is the percentage of single drug B in the compound bactericide; A. b is EC of two single drugs respectively 50 Value, mg.L -1 。
SR＝X 1 /X 2 (2)
Wherein X is 1 Is a compound bactericide EC 50 Theoretical value, mg.L -1 ；X 2 Is a compound bactericide EC 50 Actual value, mg.L -1 。
The combined action type of the compound bactericide is evaluated by comparing SR, the SR is more than 1.5 and is a synergistic effect, the SR is more than or equal to 0.5 and less than or equal to 1.5 and is an additive effect, and the SR is less than 0.5 and is an antagonistic effect, so that the medicament effect is evaluated.
The indoor toxicity of the tested bactericides to hyphae was tested by a flat-plate hypha growth rate method. Each treatment was set to 5 replicates and the non-dosed group was CK (control).
Hypha growth rate (mm.d) -1 ) Inner-outer circle distance/two scribe interval time;
relative inhibition of hypha growth = (non-dosed group hypha growth rate)/non-dosed group hypha growth rate x 100%.
The results are shown in tables 1 and 2.
TABLE 1 EC of the compounded bactericides on the growth of M.species filaments 50
The inhibition effect of the three concentrations of the compound bactericide on the growth of the staphylococcus aureus, which is measured by using a hypha growth rate method, is shown in table 1, and the prochloraz manganese chloride salt is 20:1 for EC of the staphylococcus aureus 50 The minimum value is 0.496 mg.L -1 . EC of three compound bactericides (20:1, 30:1, 40:1) 50 The values are smaller than that of the two single medicines, and the synergistic effect, the synergistic effect and the additive effect are respectively shown according to the synergistic coefficient, wherein the synergistic coefficient of the prochloraz-manganese chloride salt and the octreotide acetate is the maximum when the weight ratio of the prochloraz-manganese chloride salt to the octreotide acetate is 20:1.
TABLE 2 Effect of the Compound bactericides on the growth of agaricus bisporus mycelium
Note that the different lower case letters in the table for the same column indicate significant differences between the different treatments (P < 0.05).
The results of the effects of the three compound bactericides on the growth of the three agaricus bisporus hyphae are shown in table 2, and the results show that the three compound bactericides have a certain effect on the growth of the agaricus bisporus hyphae, wherein the effects of the ratio of 20:1 and 30:1 on the growth of the agaricus bisporus hyphae are small, and the difference is not obvious compared with a control group. The inhibition effect on the agaricus bisporus mycelium is obviously different from that of a control when the ratio is 40:1, which shows that the compound bactericide in the ratio has obvious inhibition on the growth of the agaricus bisporus mycelium.
Safety determination of compound bactericide on growth of agaricus bisporus fruiting body
On the basis of selecting the optimal proportion in the indoor toxicity measurement, the optimal synergistic proportion of prochloraz-manganese chloride complex and octreotide acetate is selected to be 20:1 for test, and the bactericide test is 1 time, 2 times and 4 times of 3 concentrations of the recommended dosage in the field according to the requirements of pesticide safety evaluation criterion (NY/T1965.1-2010). The results of the test were investigated after 12d by spraying treatment when the mushroom bud was raised from the cover soil. The cells are arranged by adopting random block group, each treatment is repeated for 3 times, and the area of each cell is 2m 2 . Each cell was collected 20 fruit bodies weighed using a five-point sampling method.
The effect of the compound bactericide on the growth of the agaricus bisporus fruiting body is shown in Table 3, and 105 mg.L when prochloraz manganese chloride and octreotide acetate are compounded according to the weight ratio of 20:1 -1 、210mg·L -1 And 420 mg.L -1 Under the three treatment doses, the method has no adverse effect on the yield of the fruiting bodies of the agaricus bisporus, has no obvious difference with a control, does not find the phytotoxicity symptoms such as necrosis, deformity and the like, and shows that the compounding of prochloraz manganese and octreotide acetate has high toxicity on inhibiting the trichoderma bisporus and is safe for the growth of the agaricus bisporus.
TABLE 3 Effect of the Compound bactericides on yield of agaricus bisporus
Note that: the yield is the fresh weight (unit: g) of every 20 fruiting bodies of the agaricus bisporus treated differently; the different lower case letters of the same column in the table indicate that the differences between the different treatments are significant (P < 0.05).
Field efficacy test of compound bactericide on agaricus bisporus cobweb disease
The field spray prevention effect test of the compound bactericide on the agaricus bisporus arachnoid disease is carried out, the selected test field is a mushroom shed for planting agaricus bisporus in successive years, and the agaricus bisporus variety is S2796. The test is divided into 5 agent treatments: the dosage of the active ingredients of prochloraz manganese chloride + octylamine acetate (20:1) is 52.5, 78.75 and 105 mg.L -1 Two single-dose treatment of 50% prochloraz manganese chloride wettable powder 200 mg.L -1 1.8% octyl fungus amine acetate aqua 5 mg.L -1 Setting clear water treatment as control, and applying the drug amount of each treatment to be 250mL/m 2 Spraying water. The cells are arranged by adopting random block group, each treatment is repeated for 3 times, and the area of each cell is 2m 2 。
According to pesticide field efficacy test criterion (NY-T1464.10-2007), the occurrence of diseases is investigated, the number of all the mushrooms in each district is recorded, the incidence of bisporus is recorded, and the control effect of the pesticide is calculated. About 10d after the application is the harvesting period of the agaricus bisporus, and the test results are investigated.
The drug effect calculation method comprises the following steps: p= (CK-PT)/ck×100
Wherein: p-control effect in percent (%);
CK-number of mushrooms (clusters) in the control area;
PT-number of mushrooms (clusters) in the treatment area with the agent.
The culture medium was sprayed when the mushroom buds were in place 10d after the soil was covered, and the test results were investigated 10d later. The results of the field efficacy test are shown in table 4. As can be seen from Table 4, the results of the field efficacy test of the prochloraz manganese chloride and the octreotide acetate compound show that the prochloraz manganese chloride and the octreotide acetate are mixed according to a ratio of 20:1, and the treatment concentration is 105 mg.L -1 The prevention and treatment effect on the agaricus bisporus cobweb disease is best, the prevention and treatment effect can reach 86.37 percent, and the dosage is reduced by half compared with the single application of prochloraz manganese. Prochloraz manganese chloride complex and octylamine acetate 20:1 ratio, and the dosage of the active ingredients is 78.75 and 105 mg.L -1 Is suitable for preventing and treating spider web diseases in agaricus bisporus production. For the brown rot of the bisporous mushroom which occurs in the same period, the proportion of prochloraz manganese chloride to the octreotide acetate is 20:1, and the dosage of the active ingredients is 78.75 and 105 mg.L -1 The control effect is better than 81.5%, and the control effect is good.
Table 4 field efficacy test of the compounded bactericide against agaricus bisporus arachnoid disease
Note that the different lower case letters in the table for the same column indicate significant differences between the different treatments (P < 0.05).
Influence of compound bactericide on nutrient components of agaricus bisporus
Measuring basic nutritional components in the harvested agaricus bisporus fruiting bodies, wherein the measurement of the crude fat content is according to the method of GB/T15674-2009; crude protein content determination according to the method of GB/T15673-2009; the crude polysaccharide content is determined according to the method of NY/T1676-2008.
As is clear from Table 5, prochloraz manganese chloride and octreotide acetate were compounded at a weight ratio of 20:1, at 52.5 mg.L -1 、78.75mg·L -1 And 105 mg.L -1 Compared with CK, the three treatment doses have no adverse effect on the contents of crude fat, crude protein and crude polysaccharide in the agaricus bisporus fruiting body, and the basic nutritional ingredients of the agaricus bisporus are not obviously different between the three treatment concentrations of the compound medicament.
TABLE 5 Effect of Compound sterilant treatment on basic nutritional ingredients of agaricus bisporus
Note that the different lower case letters in the table for the same column indicate significant differences between the different treatments (P < 0.05).
The above description is only a preferred embodiment of the present invention, and is not intended to limit the invention in any way, and any person skilled in the art may make modifications or alterations to the disclosed technical content to the equivalent embodiments. However, any simple modification, equivalent variation and variation of the above embodiments according to the technical substance of the present invention still fall within the protection scope of the technical solution of the present invention.
Claims (10)
1. A bactericide composition for preventing and treating agaricus bisporus diseases is characterized in that the active ingredients are prochloraz manganese chloride and octreotide acetate, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1-40:1.
2. The fungicide composition for controlling agaricus bisporus diseases according to claim 1, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1-30:1.
3. The fungicide composition for controlling agaricus bisporus diseases according to claim 2, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1.
4. The use of the bactericidal composition of claim 1 in the preparation of a pesticide for controlling agaricus bisporus diseases, wherein the agaricus bisporus diseases are agaricus bisporus arachnoid diseases and/or agaricus bisporus brown rot diseases.
5. The pesticide for preventing and treating the agaricus bisporus arachnoid disease is characterized by comprising prochloraz manganese chloride and octreotide acetate, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1-40:1.
6. The pesticide for controlling agaricus bisporus arachnoid disease according to claim 5, wherein the weight ratio of prochloraz manganese chloride salt to octreotide acetate is 20:1-30:1.
7. A pesticide for controlling agaricus bisporus arachnoid disease according to claim 5, wherein the content of prochloraz manganese chloride salt and octreotide acetate accounts for 1-80% of the total mass of the pesticide, preferably 10.25-52.5%.
8. Use of the pesticide according to any one of claims 5 to 7 for controlling agaricus bisporus arachnoid disease caused by agaricus bisporus arachnoid disease pathogen.
9. The use according to claim 8, wherein the agaricus bisporus arachnoid pathogen is staphylococcus aureus.
10. A method for preventing and treating agaricus bisporus arachnoid disease is characterized in that pesticide for preventing and treating agaricus bisporus arachnoid disease is adopted to spray agaricus bisporus; the pesticide for preventing and treating the agaricus bisporus arachnoid comprises the active ingredients of prochloraz manganese chloride and octreotide acetate, wherein the weight ratio of prochloraz manganese chloride to octreotide acetate is 20:1.
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