CN115024331A - Bactericide composition for preventing and treating flammulina velutipes diseases and application thereof - Google Patents
Bactericide composition for preventing and treating flammulina velutipes diseases and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a bactericide composition for preventing and treating flammulina velutipes diseases and application thereof, belonging to the technical field of edible fungus disease prevention and treatment. In the bactericide composition for preventing and treating the flammulina velutipes diseases, the active ingredients are prochloraz-manganese chloride complex and xinjunan acetate, wherein the mass ratio of the prochloraz-manganese chloride complex to the xinjunan acetate is 25:1-100: 1. The pathogen of the composition is the heterocladosporium, and the composition can obviously improve the sterilization toxicity, enhance the effect, delay the drug resistance, effectively reduce the dosage of chemical pesticides, reduce the pesticide residue and environmental pollution under the compounding ratio limited by the invention, and open up a new idea for medicament development and scientific use.
Description
Technical Field
The invention belongs to the technical field of disease control of edible fungi, and particularly relates to a bactericide composition for controlling needle mushroom diseases and application thereof.
Background
In recent years, diseases on Flammulina velutipes become increasingly serious in the steaming day of the growth of Flammulina velutipes (Flammulina filiformis) planting industry, and the yield is generally reduced by 20-30% when the diseases occur. The major disease which is serious and difficult to control is the new disease of the needle mushroom arachnoid disease. The arachnoid disease of the flammulina velutipes has increasingly serious harm in mushroom sheds, and great economic loss is caused to the flammulina velutipes industry. The needle mushroom arachnoid disease is a disease caused by infection of heterotypic dextranum (Cladobotryum variaum), and can also infect various edible fungi such as shiitake mushroom, pleurotus eryngii, pleurotus ostreatus, ganoderma lucidum and the like besides needle mushroom. The moldy mycelium generated at the early stage of the disease adheres to the soil cover and then gradually spreads from the junction of the soil cover and the stipe to the top of the fruiting body. Pathogenic bacteria mycelium is cotton-shaped gradually, a large number of spores are generated simultaneously, diseased needle mushroom fruiting bodies are brown spots and are rotted finally, the whole fruiting bodies can be withered when the disease is serious, and the pathogenic bacteria can infect and attack the needle mushroom in the whole development period. As most medicaments can inhibit the growth of hyphae of large-scale fungi flammulina velutipes while inhibiting the growth of hyphae of pathogenic bacteria, the adverse effects on the yield and the quality of the flammulina velutipes are caused, and no high-efficiency and safe registered medicament is produced at present for preventing and treating the arachnoid disease of the flammulina velutipes.
The prochloraz-manganese chloride salt has the chemical name of N-propyl-N- [2- (2,4, 6-trichlorophenoxy) ethyl ] -1H imidazole-1-formamide-manganese chloride and is prepared by compounding prochloraz and manganese chloride. The prochloraz manganese has multiple functions of systemic absorption, conduction, prevention, protection, treatment and the like, belongs to imidazole bactericides, has the function of inhibiting the biosynthesis of sterol, has good effect of preventing various diseases caused by ascomycetes and imperfect fungi, and is registered in China for preventing and treating diseases such as mushroom brown rot, blister disease and the like.
The Xinjunan acetate is an environment-friendly amino acid high-molecular polymer bactericide. The Xinjunan acetate has a wide sterilization range, has certain sterilization and bacteriostasis effects on fungi, bacteria and virus diseases, has a main sterilization mechanism that ionization is generated in an aqueous solution, and a hydrophilic group of the Xinjunan acetate has strong electropositivity so as to adsorb various pathogens which are usually electronegative and propagate the pathogens, and a film formed by a polymer has the effect of blocking a microorganism ion channel, so that an ideal sterilization effect is achieved. The Xinjunan acetate has no cross resistance with a common bactericide and has the function of promoting the growth of crops.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a bactericide composition for preventing and treating flammulina velutipes diseases and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a bactericide composition for preventing and treating flammulina velutipes diseases comprises the effective components of prochloraz manganese and xinjunan acetate, wherein the mass ratio of the prochloraz manganese to the xinjunan acetate is 25:1-100: 1.
On the basis of the scheme, the mass ratio of the prochloraz manganese salt to the Xinjunan acetate is preferably 25:1-50:1, and more preferably 25: 1.
The bactericide composition for preventing and treating the diseases of the flammulina velutipes is applied to the preparation of pesticides for preventing and treating the diseases of edible fungi, wherein the edible fungi are flammulina velutipes, lentinus edodes, pleurotus eryngii, pleurotus ostreatus or lucid ganoderma.
A pesticide for preventing and treating edible fungus arachnoid diseases comprises the effective components of prochloraz manganese salt and Xinjunan acetate, wherein the weight ratio of the prochloraz manganese salt to the Xinjunan acetate is 25:1-100: 1. Wherein the pathogeny of the edible fungus arachnoid disease aiming at the pesticide for preventing and treating the edible fungus arachnoid disease is heterocladosporium.
The pesticide for preventing and treating the edible fungus arachnoid disease also comprises auxiliary agents which are used for conveniently using or uniformly distributing the effective components of prochloraz-manganese chloride complex and Xinjunan acetate on crops, such as inert fillers, wetting dispersants, penetrants, emulsifiers, solvents, antifreezing agents, antifoaming agents, warning colors and the like, and the auxiliary agents can be solid and liquid additives which are conventionally used in the formula of the prior art.
In the presence of the conventional auxiliary agent, the pesticide can be prepared into any one of wettable powder, water dispersible granules, suspending agents and aqueous emulsion.
The content of the prochloraz manganese salt and the Xinjunan acetate in the pesticide for preventing and treating the edible fungus arachnoid diseases accounts for 1-80 percent of the total mass of the pesticide, and the preferable content is 20.2-52 percent.
The pesticide is applied to preventing and treating the edible fungus arachnoid diseases.
A method for preventing and treating cobweb disease of edible fungi comprises spraying the above pesticide to edible fungi; wherein the pathogen of the edible fungus arachnoid disease is heterocladosporium heterocladospermi.
The bactericidal composition can also be used for preventing and treating agaricus bisporus brown rot, mushroom white rot and the like.
The invention has the following advantages:
the invention provides a composition for preventing and treating edible fungus arachnoid diseases by mixing environment-friendly high molecular polymer Xinjunan acetate and a chemical bactericide prochloraz manganese, aiming at the pathogen of the composition, namely heteroleptic gluglucosacchara, and the Xinjunan acetate and the prochloraz manganese can obviously improve the sterilizing toxicity, increase the effect, delay the drug resistance, effectively reduce the dosage of chemical pesticides and reduce pesticide residue and environmental pollution under the compounding ratio limited by the invention, thereby opening up a new idea for medicament development and scientific use.
Detailed Description
Terms used in the present invention have generally meanings as commonly understood by one of ordinary skill in the art, unless otherwise specified.
The present invention will be described in further detail with reference to the following data in conjunction with specific examples. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.
In the following examples, a raw pesticide of prochloraz manganese was purchased from Shandong Weifang Runfeng chemical Co., Ltd, a mother pesticide of Xinjun acetate was purchased from Weifang Wansheng biopesticide Co., Ltd, a 50% wettable powder of prochloraz manganese was purchased from Shanxi Meibang pharmaceutical industry group Co., Ltd, and a 1.8% aqueous solution of Xinjun acetate was purchased from Shaxi province Xian Jia Ke agricultural chemical Co., Ltd.
The adopted flammulina velutipes arachnoid disease pathogen is cladosporium heterostemona (Cladobotryum variam) (identifying flammulina velutipes arachnoid disease pathogen, Chinese edible fungi, 2019, 38 (01): 76-79.) and is preserved by agricultural academy of sciences in Taian city.
Example 1
A bactericide composition for preventing and treating flammulina velutipes diseases comprises the effective components of prochloraz-manganese chloride complex and Xinjunan acetate, wherein the mass ratio of the prochloraz-manganese chloride complex to the Xinjunan acetate is 25: 1.
Example 2
A bactericide composition for preventing and treating flammulina velutipes diseases comprises the effective components of prochloraz-manganese chloride complex and Xinjunan acetate, wherein the mass ratio of the prochloraz-manganese chloride complex to the Xinjunan acetate is 50: 1.
Example 3
The bactericide composition for preventing and treating the flammulina velutipes diseases comprises the effective components of prochloraz manganese and Xinjunan acetate, wherein the mass ratio of the prochloraz manganese to the Xinjunan acetate is 100: 1.
Combined toxicity of prochloraz-manganese chloride complex and Xinjunan acetate on heteroleptic glume
A hypha growth rate method is adopted to determine indoor toxicity of prochloraz manganese salt and Xinjunan acetate to flammulina velutipes arachnoides heterocladosporium (C.variam) after being used independently and in a mixed manner according to the ratio of 25:1, 50:1 and 100: 1: diluting the medicine to 5-7 concentrations, mixing with PDA culture medium, pouring into 9cm plate, cooling to obtain medicine-containing plate, taking culture medium with equal amount of sterilized water as control, inversely inoculating 7mm cake of Flammulina velutipes Nephila (heterocladosporium), culturing at 28 deg.C until the control grows over 80% of the plate, measuring colony diameter by cross method,growth inhibition rate was calculated and EC was calculated with software 50 The combined toxicity of the two-agent compound on the growth of heterocladosporium wires is evaluated by a Wadley method, and the synergistic coefficient (SR) is calculated. SR > 1.5 is synergistic effect, SR < 0.5 is additive effect, SR < 0.5 is antagonistic effect, and the results are shown in Table 1.
TABLE 1 Combined toxicity of prochloraz-manganese chloride complex and Xinjunan acetate on flammulina velutipes arachnoids germ
| Medicament | Linear regression equation | EC 50 (mg/L) | Coefficient of efficiency (SR) |
| Prochloraz manganese salt (A) | y＝0.562+1.170x | 0.331 | / |
| Xinjunan acetate (B) | y＝1.452+1.033x | 0.039 | / |
| A:B＝25:1 | y＝＝1.291+1.595x | 0.155 | 1.66 |
| A:B＝50:1 | y＝1.05+1.454x | 0.190 | 1.52 |
| A:B＝100:1 | y＝0.939+1.404x | 0.214 | 1.44 |
As can be seen from Table 1: EC of prochloraz-manganese chloride complex and Xinjunan acetate single dose on heterotypic cladosporium 50 Respectively 0.331mg/L and 0.039mg/L, and the EC of the heterocladosporium after the two are mixed according to the mass ratio of 25:1-100:1 50 Respectively being 0.155mg/L, 0.190mg/L and 0.214mg/L, and the synergistic coefficients thereof being 1.66, 1.52 and 1.44, wherein when the prochloraz manganese salt and the Xinjunan acetate are mixed according to the mass ratio of 25:1 and 50:1, the prochloraz manganese salt and the Xinjunan acetate have a synergistic effect on the heteroleptic glume.
Safety of compounding prochloraz-manganese chloride complex and Xinjunan acetate on flammulina velutipes
1. Safety to growth of flammulina velutipes hyphae
Measuring indoor bioactivity of the compound bactericide on Flammulina velutipes mycelium by mycelium growth rate method, dissolving the above components according to indoor toxicity measuring method, diluting with sterile water, and making into 1 × 10 4 And (5) storing the mother liquor of mg/L in a refrigerator at 4 ℃ for a short time for later use.
Taking 45mL of PDA culture medium prepared in advance, quantitatively adding into a 100mL triangular flask sterilized in advance and dried, sequentially adding 5mL of the prepared mother solution from low concentration to high concentration, fully shaking, pouring into 3 culture dishes with the diameter of 9cm in equal amount, and respectively preparing into EC for pathogenic bacteria 50 Value, 2 times EC 50 Value and 4 times EC 50 The plate was plated at 3 treatment concentrations and was ready for use. Treatments without agent were included as blanks, with 3 replicates per treatment. Taking activated needle mushroom mycelia under aseptic condition, and respectively usingThe sterilized punch is used for punching a fungus cake with the diameter of 7mm from the edge of the vigorously growing hypha, inoculating the fungus cake to the center of the prepared medicine-containing flat plate, sealing, and placing the flat plate into a constant-temperature incubator at the temperature of 25 +/-1 ℃ for dark culture. The colony diameter was measured by crosshatch when the blank control hyphae grew to petri dish 3/4 and the growth inhibition was calculated and compared to the drug-free blank control.
The results are shown in table 2: a formula of prochloraz manganese salt (A) and Xinjunan acetate (B) in EC 50 Value, 2 times EC 50 The inhibition effect on the growth of the flammulina velutipes hyphae is not obvious under various treatment concentrations, the growth of the flammulina velutipes hyphae is safe, and the EC is only 4 times that of a 50:1 formula 50 The value treatment has certain inhibition on the growth of hyphae.
TABLE 2 safety of prochloraz-manganese chloride complex (A) and Xinjunan acetate (B) on Flammulina velutipes hyphae
2. Yield influence of Flammulina velutipes
According to the requirements of 'evaluation criteria of pesticide on crop safety' (NY/T1965.1-2010), the bactericide safety test has 3 concentrations which are 1 time, 2 times and 4 times of the field recommended dosage and are all the dosage of the effective components. And (4) when the mushroom buds are fully distributed in the bag head, detaching the bag head for spraying treatment, and investigating the test result after 7 d. The cells are arranged in random block groups, each treatment is repeated for 3 times, and the area of each cell is 2m 2 And collecting 10 cultivation bags in each cell by adopting a five-point sampling method for investigation.
TABLE 3 Effect of Prochloraz manganese salt (A) and Xinjunan acetate (B) on Flammulina velutipes yield
| Medicament | The effective component dosage (mg/L) | Yield (g) |
| A:B＝25:1 | 104 | 625.8bc |
| A:B＝25:1 | 208 | 633.1bc |
| A:B＝25:1 | 416 | 623.8bc |
| A:B＝50:1 | 102 | 617.8bc |
| A:B＝50:1 | 204 | 712.5a |
| A:B＝50:1 | 408 | 702.8a |
| A:B＝100:1 | 101 | 604.7c |
| A:B＝100:1 | 202 | 671.1ab |
| A:B＝100:1 | 404 | 661.0bc |
| Clear water control | —— | 627.5bc |
Note: the yield is the fresh weight (unit: g) of the first tide of Flammulina velutipes in each 10 cultivation bags treated differently
The results are shown in Table 3: compared with the clear water control, the yield of the needle mushroom fruiting body treated by the three formulas of the prochloraz-manganese chloride complex (A) and the Xinjunan acetate (B) in the concentration of 1 time, 2 times and 4 times of the field application dose is not obvious in inhibition effect, no phytotoxicity symptoms such as necrosis and deformity are found, and the yield is promoted to a certain extent by the medium-high dose of 50:1 treatment. The three compound medicaments of the prochloraz-manganese chloride complex and the Xinjunan acetate can be safely used on the flammulina velutipes under the test dosage.
Example 4
A pesticide for preventing and treating edible fungus arachnoid diseases contains 26% of prochloraz manganese salt and Xinjunan acetate (25:1) as effective components.
The raw materials comprise the following components in parts by weight: 25 parts of prochloraz manganese, 1 part of Xinjunan acetate, 7 parts of a wetting agent and the balance of a filling agent to 100 parts.
The preparation method comprises the following steps: weighing prochloraz-manganese chloride complex, Xinjunan acetate, a wetting agent and a filler (any one of white carbon black, kaolin and diatomite), adsorbing the effective ingredients by the filler, uniformly mixing, and then carrying out airflow pulverization to prepare the wettable powder with the content of the effective ingredients of 26%.
The wettable powder can be prepared into water dispersible granules by granulation.
The wettable powder can be processed into a suspending agent by adding water and grinding by sanding.
Example 5
A pesticide for preventing and treating edible fungus arachnoid disease contains 51% of prochloraz manganese salt and Xinjunan acetate (50:1) as effective components.
The raw materials comprise the following components in parts by weight: 50 parts of prochloraz manganese salt, 1 part of Xinjunan acetate, 7 parts of wetting agent and the balance of filler to 100 parts.
The preparation method comprises the following steps: weighing prochloraz-manganese chloride complex, Xinjunan acetate, a wetting agent and a filler (any one of white carbon black, kaolin and diatomite), adsorbing the effective ingredients by the filler, uniformly mixing, and carrying out air flow crushing to prepare the wettable powder with the effective ingredient content of 51%.
Example 6
A pesticide for preventing and treating edible fungus arachnoid disease contains 50.5% of prochloraz-manganese chloride complex and Xinjunan acetate (100: 1).
The raw materials comprise the following components in parts by weight: 50 parts of prochloraz-manganese chloride complex, 0.5 part of Xinjunan acetate, 7 parts of a wetting agent and the balance of a filler to 100 parts.
The preparation method comprises the following steps: weighing prochloraz-manganese chloride complex, Xinjunan acetate, a wetting agent and a filler (any one of white carbon black, kaolin and diatomite), adsorbing the effective ingredients by the filler, uniformly mixing, and then carrying out airflow pulverization to prepare the wettable powder with the effective ingredient content of 50.5%.
Xinjunan acetate and prochloraz manganese chloride complex for field control of flammulina velutipes arachnoid disease
The method is characterized in that the Xinjunan acetate and the Prochloraz manganese salt are respectively processed into wettable powder with the effective components of 26% of Prochloraz manganese salt and Xinjunan acetate (25:1), wettable powder with the effective components of 51% of Prochloraz manganese salt and Xinjunan acetate (50:1) and wettable powder with the effective components of 50.5% of Prochloraz manganese salt and Xinjunan acetate (100:1), and the wettable powder with the effective components of 50% of Prochloraz manganese salt and 1.8% of Xinjunan acetate aqueous solution are used as comparison preparations for preventing and treating the flammulina velutipes arachnoid disease in the field.
Planting needle mushroom in a mushroom shed for continuously planting needle mushroom for many years, wherein the cultivation formula comprises 40% of mixed sawdust and cottonseed hull30 percent of bran, 18 percent of corn flour, 1 percent of sugar, 1 percent of calcium superphosphate, 1 percent of gypsum and 1 percent of lime, a 17.5cm x 33cm plastic bag is used, the filling height is about 15cm, a hole is punched in the middle of a fungus bag to increase the fungus growing speed and consistency, the inoculation hole does not collapse, and the depth and the height are consistent. After filling, the fungus bags are sleeved with plastic collars, cotton plugs are plugged, and a space of about 10cm is reserved between the cotton plugs and the material surface. The prepared fungus bags are uniformly placed in a turnover basket, and cotton plugs of the fungus bags are required to be placed in order. After bagging, the fungus bags are put into a pot for sterilization. And (4) cooling the sterilized fungus bags in a cooling chamber, and maintaining the positive pressure of the cooling chamber by using a high-efficiency air filter to prevent the contamination of mixed fungi. And (4) artificially inoculating 30-100 bags of the strain bags when the strain bags are cooled to below 20 ℃. Culturing the inoculated fungus bag in a culture room at 20-22 deg.C and relative humidity of 65-70% in dark place. The cotton plug of the fungus bag faces upwards during culture. And (3) transferring the needle mushroom bags with developed mushrooms out of the mushroom shed, wherein the temperature in the shed is 8-15 ℃, and the relative air humidity is 85-95%. When primordia appears about 10 days after the opening, spore suspension (1 × 10) of needle mushroom arachnoid disease prepared in advance 6 one/mL) was sprayed into each treatment bag, each bag being sprayed with 2mL-3mL of spore suspension. And (3) folding the bag mouth of the fungus bag downwards to expose the fungus buds after the fungus buds appear for about 3 days, respectively weighing the test concentration required by each cell, carrying out spray treatment on the fungus buds of the needle mushrooms from low concentration to high concentration, keeping the temperature and humidity, covering by using a black film, and collecting 75 cultivation bags in each cell by using a five-point sampling method for investigation.
According to the NY-T1464.10-2007 pesticide field efficacy test criteria, disease occurrence conditions are investigated, the number of all diseased mushrooms in each cell is recorded, the incidence rate of flammulina velutipes is recorded, and the control effect of the pesticide is calculated. And (5) after the application, the harvesting period of the flammulina velutipes is about 7 days, and the test result is investigated. And (4) carrying out variance analysis on the control effect, and comparing whether the average control effect of each medicament has significant difference by adopting a Duncan's new repolarization method.
The drug effect calculation method comprises the following steps: p ═ CK-PT)/CK × 100
In the formula: p-control effect, unit is percentage (%);
CK-number of diseased mushrooms (colonies) in the blank control area; PT-number of diseased mushrooms (fungus mass) in the medicament treatment area.
TABLE 4 prevention effect of combination of Xinjunan acetate and prochloraz manganese on flammulina velutipes arachnoid disease
The results are shown in table 4: compared with 400mg/L of 50% prochloraz-manganese chloride wettable powder used in fields for preventing and treating flammulina velutipes arachnoid diseases, the control effects of 26% prochloraz-manganese chloride + Xinjunan acetate (25:1) wettable powder, 51% prochloraz-manganese chloride + Xinjunan acetate (50:1) wettable powder and 50.5% prochloraz-manganese chloride + Xinjunan acetate (100:1) wettable powder are remarkably higher than the control effects of prochloraz-manganese chloride alone, and the dosage of chemical bactericide prochloraz-manganese chloride 3/4 is reduced by less than that of the chemical bactericide prochloraz-manganese chloride. The control effect of the 26% prochloraz manganese salt and Xinjunan acetate (25:1) wettable powder is the highest, the control effect of the 26% prochloraz manganese salt and Xinjunan acetate (50:1) wettable powder is equivalent to that of the 51% prochloraz manganese salt and Xinjunan acetate (50:1) wettable powder, and the control effect is obviously higher than that of 50.5% prochloraz manganese salt and Xinjunan acetate (100:1) wettable powder treatment and prochloraz manganese salt single treatment. The result shows that the combination of the Xinjunan acetate and the prochloraz manganese can reduce the dosage of the chemical fungicide prochloraz manganese and improve the control effect, and is suitable for field control of the flammulina velutipes arachnoid disease.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (10)
1. The bactericide composition for preventing and treating the flammulina velutipes diseases is characterized by comprising the effective components of prochloraz manganese and Xinjunan acetate, wherein the weight ratio of the prochloraz manganese to the Xinjunan acetate is 25:1-100: 1.
2. The bactericide composition for preventing and treating flammulina velutipes diseases according to claim 1, wherein the weight ratio of the prochloraz manganese salt to the Xinjunan acetate is 25:1-50: 1.
3. The bactericide composition for preventing and treating flammulina velutipes diseases according to claim 2, wherein the weight ratio of the prochloraz manganese salt to the Xinjunan acetate is 25: 1.
4. The use of the fungicide composition for controlling diseases of flammulina velutipes according to claim 1 in the preparation of pesticides for controlling diseases of edible fungi.
5. The use according to claim 4, wherein the edible fungi is needle mushroom, shiitake mushroom, Pleurotus eryngii, Pleurotus ostreatus or Ganoderma lucidum.
6. The pesticide for preventing and treating the edible fungus arachnoid diseases is characterized by comprising the effective components of prochloraz manganese salt and Xinjunan acetate, wherein the weight ratio of the prochloraz manganese salt to the Xinjunan acetate is 25:1-100: 1.
7. A pesticide for controlling edible fungus arachnoid disease according to claim 6, characterized in that the pathogen of the edible fungus arachnoid disease is Heterophyllus heterocladosporium.
8. The pesticide for preventing and treating the arachnoid disease of the edible fungi according to the claim 6 or 7, characterized in that the dosage form of the pesticide is selected from one of wettable powder, water dispersible granules, suspending agents and aqueous emulsion.
9. Use of the pesticide of claim 8 for controlling edible fungus arachnoid diseases.
10. A method for controlling arachnoid disease of edible fungi, characterized in that the edible fungi are sprayed with the pesticide according to claim 8; wherein the pathogen of the edible fungus arachnoid disease is heterocladosporium heterocladospermi.
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| NL2033766B1 (en) * | 2022-06-23 | 2024-01-08 | Tai An Acad Of Agricultural Sciences Tai An Branch Of Shandong Acad Of Agricultural Sciences | Fungicide composition for preventing and controlling disease of agaricus bisporus and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191969A (en) * | 2015-09-15 | 2015-12-30 | 河南科技学院 | Method for preparing special compound bactericide for oyster mushroom cultivation |
| CN105724416A (en) * | 2015-12-18 | 2016-07-06 | 河南科技学院 | Special compound fungicide for oyster mushroom raw compost cultivation and application thereof |
| CN107018799A (en) * | 2017-04-18 | 2017-08-08 | 福州大学 | The application method of Prochloraz during a kind of control planting edible mushroom |
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2022
- 2022-07-22 CN CN202210866256.5A patent/CN115024331A/en active Pending
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191969A (en) * | 2015-09-15 | 2015-12-30 | 河南科技学院 | Method for preparing special compound bactericide for oyster mushroom cultivation |
| CN105724416A (en) * | 2015-12-18 | 2016-07-06 | 河南科技学院 | Special compound fungicide for oyster mushroom raw compost cultivation and application thereof |
| CN107018799A (en) * | 2017-04-18 | 2017-08-08 | 福州大学 | The application method of Prochloraz during a kind of control planting edible mushroom |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL2033766B1 (en) * | 2022-06-23 | 2024-01-08 | Tai An Acad Of Agricultural Sciences Tai An Branch Of Shandong Acad Of Agricultural Sciences | Fungicide composition for preventing and controlling disease of agaricus bisporus and application thereof |
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| LU503702B1 (en) | 2023-09-21 |
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