LU503702B1

LU503702B1 - Fungicide composition for preventing and controlling disease of flammulina filiformis and application thereof

Info

Publication number: LU503702B1
Application number: LU503702A
Authority: LU
Inventors: Yi Kong; Yufei Lan; Qingwei Yu; Xiumei Li; Gang Chen; Qianqian Cong
Original assignee: Tai An Acad Of Agricultural Sciences Tai An Branch Of Shandong Acad Of Agricultural Sciences
Priority date: 2022-06-23
Filing date: 2023-03-21
Publication date: 2023-09-21
Also published as: CN115024331A

Abstract

The present invention discloses a fungicide composition for preventing and controlling a disease of F. filiformis and an application thereof, and belongs to the technical field of prevention and control of diseases of edible fungi. The fungicide composition for preventing and controlling a disease of F. filiformis contains prochloraz manganese salt and xinjunan acetate as active components, where a mass ratio of the prochloraz manganese salt to the xinjunan acetate is 25:1-100:1. A pathogen targeted by the composition is C. varium. The composition at the compounding ratio limited by the present invention can significantly improve fungicidal virulence, enhance efficacy, delay fungicide resistance, effectively reduce the use of chemical pesticides, lower pesticide residue and environmental pollution, and open up new ideas for development and scientific use of chemical agents.

Description

FUNGICIDE COMPOSITION FOR PREVENTING AND CONTROLLING DISEASE 8403702

FLAMMULINA FILIFORMIS AND APPLICATION THEREOF

BACKGROUND OF THE INVENTION

[0001] 1. Technical Field

[0002] The present invention belongs to the technical field of prevention and control of diseases of edible fungi, in particular to a fungicide composition for preventing and controlling a disease of F filiformis and an application thereof.

[0003] 2. Description of Related Art

[0004] In recent years, the cultivation industry of Æ filiformis has been developing rapidly.

The disease on Æfiliformis has become increasingly serious and generally reduces yield by 20%-30%. One of the diseases, which is more harmful and difficult to prevent and control is a new disease, i.e., dactylium of Æ filiformis. The harm of dactylium of Ffiliformis to the mushroom shed has become increasingly serious; as a result, great economic losses are caused to the Ffiliformis industry. The dactylium of Æ filiformis is caused by the infection of Cladobotryum varium. Besides

I filiformis, it can also infect many kinds of edible fungi such as Lentinula edodes, Pleurotus eryngii, Pleurotus ostreatus and Ganoderma lingzhi. At an initial stage of the disease, downy mildew-like mycelia growing out attach to overlying soil and slowly spread from the junction of the overlying soil and the fungal stalk to the top of the fruiting body. The mycelia of the pathogen gradually show cotton floc shape and also produce a large number of spores. The fruiting body of the diseased Æ filiformis has brown spots and finally rots, and in a severe case, the whole fruiting bodies atrophies and the dactylium can infect during the whole development period of Ffiliformis.

Most chemical agents not only inhibit the mycelium growth of the pathogen, but also inhibit the mycelium growth of the macro fungi Æ filiformis, thus adversely affecting the yield and quality of /< filiformis. Therefore, there is no effective and safe registered chemical agent for the prevention and control of dactylium of Æfiliformis in production.

[0005] Prochloraz manganese salt has a chemical name of N-propyl-N - [#503702 (2,4,6-trichlorophenoxy) ethyl] - 1H imidazol-1-formamide - manganese chloride and is formed through compounding of prochloraz and manganese chloride. Prochloraz manganese salt has multiple actions such as internal absorption, conduction, prevention, protection, treatment, etc. It belongs to imidazole fungicides and acts by inhibiting the biosynthesis of sterols. In addition, it has a better prevention effect on many diseases caused by ascomycetes and imperfect fungi, and is registered in China for the prevention and control of diseases such as mushroom brown rot and wet bubble disease.

[0006] Xinjunan acetate is a kind of environment-friendly amino acids polymer fungicide.

Xinjunan acetate has a wide range of sterilization, and has certain sterilization and bacteriostatic effects on fungi, bacteria and virus diseases. Its main sterilization mechanism is to ionize in the aqueous solution, and its hydrophilic group contains strong positive electricity, so as to adsorb various kinds of pathogens that are usually negatively charged and inhibit the propagation of pathogenic bacteria. In addition, a film formed by the polymer has the effect of blocking the microbial ion channel to achieve an ideal sterilization effect. There is no cross-resistance between xinjunan acetate and common fungicides, and xinjunan acetate can promote the growth of crops.

BRIEF SUMMARY OF THE INVENTION

[0007] In view of the problems existing in the prior art, the present invention aims to provide a fungicide composition for preventing and controlling a disease of Æ filiformis and an application thereof.

[0008] In order to achieve the above objective, the present invention adopts the following technical solution:

[0009] A fungicide composition for preventing and controlling a disease of H filiformis contains prochloraz manganese salt and xinjunan acetate as active components, where a mass ratio of the prochloraz manganese salt to the xinjunan acetate is 25:1-100:1.

[0010] On the basis of the above solution, the mass ratio of the prochloraz manganesé 843/702 to the xinjunan acetate is preferably 25:1-50:1, more preferably 25:1.

[0011] The application of the fungicide composition for preventing and controlling the disease of F. filiformis in the preparation of pesticides for preventing and controlling the diseases of edible fungi, where the edible fungi are Æ filiformis, Lentinula edodes, Pleurotus eryngii, Pleurotus ostreatus or Ganoderma lingzhi.

[0012] A pesticide for preventing and controlling edible fungus dactylium contains prochloraz manganese salt and xinjunan acetate as active components, where a weight ratio of the prochloraz manganese salt to the xinjunan acetate is 25:1-100:1. Where the pathogen of edible fungus dactylium targeted by the pesticide for preventing and controlling edible fungus dactylium is

C. varium.

[0013] The pesticide for preventing and controlling edible fungus dactylium further contains auxiliaries for convenient use or uniform distribution of the active components prochloraz manganese salt and xinjunan acetate on crops, such as inert fillers, wetting and dispersing agents, penetrating agents, emulsifiers, solvents, antifreeze agents, defoamers, warning color pastes, and the like, and the auxiliaries can be solid and liquid additives routinely used in formulations of the prior art.

[0014] In the presence of the above conventional auxiliaries, the pesticide can be prepared into any one of a wettable powder, a water dispersible granule, a suspending agent and an water emulsion.

[0015] In the pesticide for preventing and controlling edible fungus dactylium, the content of the prochloraz manganese salt and the xinjunan acetate accounts for 1-80% of a total mass of the pesticide, preferably 20.2-52%.

[0016] The pesticide is applied to preventing and controlling edible fungus dactylium.

[0017] In a method for preventing and controlling edible fungus dactylium, the edible fungi are spray with the above pesticide, where the pathogen of edible fungus dactylium is C.

varium. LU503702

[0018] The fungicide composition can also be used to prevent and control the brown rot of agaricus bisporus, white rot of mushroom, etc.

[0019] The present invention has the following advantages:

[0020] the present invention provides a composition in which an environmentally friendly polymer xinjunan acetate and a chemical fungicide prochloraz manganese salt are mixed to prevent and control edible fungus dactylium. The pathogen targeted by the composition is C. varium. The composition at the compounding ratio, limited by the present invention, of xinjunan acetate and prochloraz manganese salt can significantly improve fungicidal virulence, enhance efficacy, delay fungicide resistance, effectively reduce the use of chemical pesticides, lower pesticide residue and environmental pollution, and open up new ideas for development and scientific use of chemical agents.

DETAILED DESCRIPTION OF THE INVENTION

[0021] The terms used in the present invention, unless otherwise specified, generally have meanings normally understood by those of ordinary skills in the art.

[0022] The present invention is further described in detail in combination with particular embodiments and with reference to data. The following examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention in any way.

[0023] In the following embodiments, the original drug of prochloraz manganese salt was purchased from Shandong Weifang Rainbow Chemical Co., Ltd., the technical concentrate of xinjunan acetate was purchased from Weifang Voelsing Biopesticide Co. Ltd., 50% prochloraz manganese salt wettable powder was purchased from Shanxi Meibang Pharmaceutical Group Co,

Ltd, and 1.8% xinjunan acetate aqueous agent was purchased from Shanxi Xi'an Jiake

Agro-Chemical Co., Ltd.

[0024] The pathogen of F filiformis dactylium is C. varium (identification of the pathogen of I filiformis dactylium, Chinese Edible Fungi, 2019, 38 (01): 76-79.), whid/583702 preserved by the Tai'an Academy of Agricultural Sciences.

[0025] Embodiment 1

[0026] A fungicide composition for preventing and controlling a disease of Æ filiformis 5 contain prochloraz manganese salt and xinjunan acetate as active components, where a mass ratio of the prochloraz manganese salt to the xinjunan acetate was 25:1.

[0027] Embodiment 2

[0028] A fungicide composition for preventing and controlling a disease of Æ filiformis contains prochloraz manganese salt and xinjunan acetate as active components, where a mass ratio ofthe prochloraz manganese salt to the xinjunan acetate was 50:1.

[0029] Embodiment 3

[0030] A fungicide composition for preventing and controlling a disease of Æ filiformis contains prochloraz manganese salt and xinjunan acetate as active components, where a mass ratio of the prochloraz manganese salt to the xinjunan acetate was 100:1.

[0031] Joint toxicity of prochloraz manganese salt + xinjunan acetate to C. varium

[0032] The mycelial growth rate method was used to determine the indoor toxicity of prochloraz manganese salt and xinjunan acetate to C. varium, namely pathogen of FE filiformis dactylium, after prochloraz manganese salt and xinjunan acetate being used independently and mixed at 25:1, 50:1 and 100:1: the chemical agent was diluted to 5-7 concentrations, mixed with

PDA culture medium to obtain a mixture, and thenthe mixture was pour into a 9-cm plate. After cooling, a drug-containing plate was made and was compared with the culture medium containing the same amount of sterile water, then, the 7-mm mycelium cake (C. varium) of pathogen of dactylium of F filiformis was reversely inoculated in the center of the drug-containing plate, and was cultured at 28 °C until the culture medium of the control group accounts for 80% of the plate for determination, the colony diameter was measured with the cross method, the growth inhibition rate was calculated and ECso was calculated with software, the combined toxicity of compounding of the two chemical agents was evaluated for the growth of the mycelium of C. varium by-4f83702

Wadley method, and the synergistic ratio (SR) was calculated. SR > 1.5 indicates the synergistic effect, 0.5 < SR < 1.5 indicates the additive effect, and SR < 0.5 indicates the antagonistic effect.

The results are shown in Table 1.

Table 1 Combined toxicity of Mixed Application of Prochloraz Manganese Salt and Xinjunan

Acetate to Dactylium of FE. filiformis

[0033] It can be seen from Table 1 that ECso of each of prochloraz manganese salt and xinjunan acetate to C. varium is 0.331 mg/L and 0.039 mg/L respectively, and the ECso of prochloraz manganese salt and xinjunan acetate after being mixed according to the mass ratio of 25:1 to 100:1 to C. varium is 0.155 mg/L, 0.190 mg/L and 0.214 mg/L respectively, and their synergistic coefficients are 1.66, 1.52 and 1.44 respectively, wherein, when prochloraz manganese salt and xinjunan acetate are mixed according to the mass ratio of 25:1 and 50:1, they have synergistic effect on C. varium.

[0034] Safety of compounding of prochloraz manganese salt + xinjunan acetate to F filiformis

[0035] 1. Safety to mycelial growth of F. filiformis

[0036] The mycelial growth rate method was used to determine the indoor biological activity of the compound fungicide against the Æ filiformis mycelium. After the various formulations were dissolved according to the indoor toxicity test method, they were diluted with sterile water and prepared into 1 x10* mg/L mother liquor which was stored in a refrigerator at 4 °C for short term and standby application. LU503702

[0037] 45 mL of PDA culture medium prepared in advance was taken and quantitatively added into a 100-mL pre-sterilized and dry triangular flask, 5 mL of the prepared mother liquor was added from low concentration to high concentration, the triangular flask was fully shaken, and then the solution was poured into three culture dishes with a diameter of 9 cm in equal amount, and the culture medium plates with three treatment concentrations of ECso, 2-times ECso value and 4-times

ECso for germs respectively were prepared for standby application. The treatment without chemical agents was set as blank control, and each treatment was repeated three times. Under sterile operation conditions, the activated F. filiformis mycelium was taken, a sterilized punch was respectively used to punch a Æ filiformis cake having the diameter of 7 mm from the edge of the vigorous mycelium, the cake was inoculated in the center of the prepared drug-containing plate, the plate was sealed, and then the plate was put into a constant temperature incubator at 25 + 1 °C for dark culture. When the mycelium of the blank control grew to 3/4 of the culture dish, the cross method was used to measure the colony diameter, the growth inhibition rate was calculated, and the growth inhibition rate was compared with that of the blank control without chemical agents.

[0038] The results are as shown in Table 2: the compound formula of prochloraz manganese salt (A) and xinjunan acetate (B) has no significant difference in inhibiting the mycelial growth of F. filiformis at ECso and 2-times ECso, is safe for the mycelial growth of I filiformis, and only has certain inhibition on the mycelial growth in the treatment of 4-times ECso of the 50:1 formula.

Table 2 Safety of Mixed Application of Prochloraz Manganese Salt (A) and Xinjunan Acetate (B) to Mycelium of FE filiformis [es | ew | =

AB =50:1 0.190 5.31 ab 7505702

AB=501 0580 52706

A:B =100:1 0.214 5.42 ab

A:B =100:1 0.428 5.34 ab

A:B =100:1 0.856 5.29 ab

[0039] 2. Influence to yield of Æ filiformis

[0040] According to the requirements of Guidelines for Crop Safety Evaluation of

Pesticides (NY/T 1965.1-2010), the concentrations of fungicides in safety test were 1, 2 and 4 times of the recommended dose in the field and all were active component dosage. When the mushroom buds were full of the bag head, the bag head was opened to spray the mushroom buds, and the test results were investigated 7 days later. All plots were arranged in randomized blocks, each treatment was repeated 3 times, and the area of each plot was 2 m*. The five-point sampling method was used to collect 10 cultivation bags from each plot for investigation.

Table 3 Influence of Mixed Application of Prochloraz Manganese Salt + Xinjunan Acetate to

Yield of F. filiformis . Dose of active

Note: the yield is the fresh weight of the first-batch mushroom of H filiformis in 10 cultivation bags under different treatments (unit: g)

[0041] The results are as shown in Table 3: the three formulations of compounding prochloraz manganese salt (A) and xinjunan acetate (B) have no significant inhibitory effect ohYf83702 yield of F. filiformis fruiting body compared with the clear water control after treatment of three concentrations of 1-time, 2-times, and 4 times of the applied dose in the field, and no symptom of chemical damage such as necrosis and deformity are found, and the medium and high doses of 50:1 treatment have a certain promotion effect on the yield. The results showed that the three compounded chemical agents of prochloraz manganese salt and xinjunan acetate could be safely used on Æ filiformis at the test dose.

[0042] Embodiment 4

[0043] A pesticide for preventing and controlling edible fungus dactylium contained 26% prochloraz manganese salt and xinjunan acetate as active components, where a weight ratio of the prochloraz manganese salt to the xinjunan acetate was 25:1.

[0044] The raw material composition of the pesticide contained, by weight, 25 parts of the prochloraz manganese salt, 1 parts of the xinjunan acetate, 7 parts of a wetting agent and the balance of a filler, totally 100 parts.

[0045] Preparation method: the prochloraz manganese salt, the xinjunan acetate, the wetting agent and the filler (any one of white carbon black, kaolin and diatomite) were weighed, the active components were first absorbed with the filler and mixed thoroughly, and then the mixture was crushed by air flow to prepare a wettable powder with a content of the active components being 26%.

[0046] The wettable powder can be prepared into a water dispersible granule by granulation.

[0047] The above wettable powder with water can be processed into a suspending agent by sand grinding.

[0048] Embodiment 5

[0049] A pesticide for preventing and controlling edible fungus dactylium contained 51% prochloraz manganese salt and xinjunan acetate as active components, where a weight ratio of the prochloraz manganese salt to the xinjunan acetate was 50:1. LUS03702

[0050] The raw material composition of the pesticide included, by weight, 50 parts of the prochloraz manganese salt, 1 parts of the xinjunan acetate, 7 parts of a wetting agent and the balance of a filler, totally 100 parts.

[0051] Preparation method: the prochloraz manganese salt, the xinjunan acetate, the wetting agent and the filler (any one of silica, kaolin and diatomite) were weighed, the active components were first absorbed with the filler and mixed thoroughly, and then the mixture was crushed by air flow to prepare a wettable powder with a content of the active components being 51%.

[0052] Embodiment 6

[0053] A pesticide for preventing and controlling edible fungus dactylium contained 50.5% prochloraz manganese salt and xinjunan acetate as active components, where a weight ratio of the prochloraz manganese salt to the xinjunan acetate was 100:1.

[0054] The raw material composition of the pesticide included, by weight, 50 parts of the prochloraz manganese salt, 0.5 parts of the xinjunan acetate, 7 parts of a wetting agent and the balance of a filler, totally 100 parts.

[0055] Preparation method: the prochloraz manganese salt, the xinjunan acetate, the wetting agent and the filler (any one of white carbon black, kaolin and diatomite) were weighed, the active components were first absorbed with the filler and mixed thoroughly, and then the mixture was crushed by air flow to prepare a wettable powder with a content of the active components being 50.5%.

[0056] Field control effect of xinjunan acetate and prochloraz manganese salt to the dactylium of Æ filiformis

[0057] Xinjunan acetate and prochloraz manganese salt were processed into wettable powder with active components of 26% prochloraz manganese salt+xinjunan acetate (25:1), 51% prochloraz manganese salt+xinjunan acetate (50:1) and 50.5% prochloraz manganese salt+xinjunan acetate (100:1) respectively, and the commercially available preparation 50% prochloraz mangaréa83702 salt wettable powder and 1.8% xinjunan acetate aqua were used as control to prevent and control the dactylium of Æ filiformis in the field.

[0058] FE filiformis was cultivated in the mushroom shed where F filiformis has been cultivated for many years. The cultivation formula was composed of 40% of weed tree sawdust, 30% of cottonseed hulls, 18% of bran, 8% of corn flour, 1% of sugar, 1% of calcium superphosphate, 1% of gypsum and 1% of lime, a plastic bag with the size of 17.5 cm x 33 cm and a loading height of about 15 cm was used, a fungus pack was perforated in the middle to increase the rate and consistency of spawn runing, and the inoculation holes did not collapse and had consistent depth and height. After loading, the fungus pack was sleeved with a plastic collar and stuffed with a cotton wool batting stopper. A space of about 10 cm was reserved between the cotton wool batting stopper and the material surface. The prepared fungus packs were uniformly placed in a turnover basket, and the cotton wool batting stoppers of the fungus packs shall be neatly placed.

After bagging, the fungus packs were put into the pot for sterilization. The sterilized fungus packs were put into a cooling room for cooling, and a high efficiency air filter was used to maintain the positive pressure of the cooling room to prevent contaminative microbes. When the fungus packs were cooled to below 20 °C, artificial inoculation was carried out on 30-100 fungus packs each time.

After inoculation, the fungus packs were put into a culture room for culture. The culture temperature was 20-22 °C, the relative humidity was 65% - 70%, and the culture was carried out in a dark place. During culture, the cotton wool batting stoppers of the fungus packs were upwards.

The Æ filiformis fungus packs after spawn runing were transferred into the mushroom shed. The temperature in the shed was 8 °C~15 °C, and the relative humidity of the air was 85-95%. About 10 days after opening, when primordia appear, spore suspension (1x10° PCS /mL) of dactylium of F filiformis prepared in advance was sprayed into each treatment fungus pack, and each fungus pack was sprayed with 2 mL to 3 mL of spore suspension. About 3 days after the mushroom bud appears, the opening of the fungus pack was folded downwards to expose the mushroom bud, the test concentration required by each plot was weighed, and the mushroom bud of F filiformis-}W}a83702 sprayed from low concentration to high concentration to maintain temperature and humidity, the mushroom buds were covered with black films, andthe five-point sampling method was used to collect 75 cultivation bags from each plot for investigation.

[0059] According to Guidelines for Field efficacy Trials of Pesticide(NY-T 1464.10-2007), the occurrence of diseases was investigated, the number of diseased mushrooms in each plot was recorded, the incidence of H filiformis was recorded and the prevention and control effects of the chemical agents were calculated. The test results were investigated in the harvest time of FE. filiformis (about 7 days after application). The control effect was analyzed by variance and

Duncan's new range test was used to compare whether there was significant difference in the average control effect of each chemical agent.

[0060] Calculation method of efficacy: P= (CK-PT) / CK x 100.

[0061] In the formula: P refers to prevention and control effect, in a unit of percentage (%).

[0062] CK refers to the number of diseased mushrooms (bacterial mass) in a blank control plot, and PT refers to the number of diseased mushrooms (bacterial mass) in a chemical-agent-treated plot.

Table 4 Control Effect of Compounding of xinjunan acetate and prochloraz manganese salt to

Dactylium of F. filiformis

Dose of active . Control

Incidence

Chemical agent component (%) effect mg/L ° % 50% prochloraz manganese salt wettable powder 1.8% xinjunan acetate saline aqueous agent 6 | 11s | 72.7b 5 — 26% prochloraz manganese salt + xinjunan acetate 25:1) wettable powder 5 — 51% prochloraz manganese salt + xinjunan acetate 85 5 ab 50:1) wettable powder 50.5% prochloraz manganese salt + xinjunan acetate 100:1) wettable powder 101 13.7 17876

Clear water control] oo | 642

[0063] The results are as shown in Table 4: compared with the 50% prochléfad3702 manganese salt wettable powder used in the field at 400 mg/L to prevent and control the dactylium of F filiformis, the control effect of each of 26% prochloraz manganese salt+xinjunan acetate (25:1) wettable powder, 51% prochloraz manganese salt+xinjunan acetate (50:1) wettable powder and 50.5% prochloraz manganese salt+xinjunan acetate (100:1) wettable powder treatment are significantly higher than that of prochloraz manganese salt used independently, and the dosage of the chemical fungicide prochloraz manganese salt was reduced to less than 3/4 when used in a compounding manner. Among them, the control effect of 26% prochloraz manganese salt+xinjunan acetate (25:1) wettable powder is the highest, is equivalent to that of 51% prochloraz manganese salt+xinjunan acetate (50:1) wettable powder, and is significantly higher than that of 50.5% prochloraz manganese salt+xinjunan acetate (100:1) wettable powder and prochloraz manganese salt used independently. This shows that the mixing of xinjunan acetate and prochloraz manganese salt can reduce the dosage of the chemical fungicide prochloraz manganese salt and improve the control effect, and is suitable for the field control of Æ filiformis dactylium.

[0064] The above description is only the preferred examples of the present invention, and is not intended to limit the present invention in other forms, and any person skilled in the art may make use of the technical contents disclosed above to change or modify the equivalent examples with equivalent changes. However, any simple modification, equivalent change and modification made in accordance with the technical essence of the invention without departing from the technical solution of the invention are still within the scope of protection of the technical solutions of the invention.

Claims

CLAIMS LU503702

1. A fungicide composition for preventing and controlling a disease of F. filiformis, containing prochloraz manganese salt and xinjunan acetate as active components, wherein a weight ratio of the prochloraz manganese salt to the xinjunan acetate 1s 25:1-100:1.

2. The fungicide composition for preventing and controlling a disease of Æ filiformis according to claim 1, wherein characterized in that a weight ratio of the prochloraz manganese salt to the xinjunan acetate is 25:1-50:1.

3. The fungicide composition for preventing and controlling a disease of Æ filiformis according to claim 2, characterized in that the weight ratio of the prochloraz manganese salt to the xinjunan acetate is 25:1.

4. An application of the fungicide composition for preventing and controlling the disease of F filiformis in the preparation of pesticides for preventing and controlling the diseases of edible fungi according to claim 1.

5. The application according to claim 4, characterized in that the edible fungi are F. filiformis, Lentinula edodes, Pleurotus eryngii, Pleurotus ostreatus or Ganoderma lingzhi.

6. A pesticide for preventing and controlling edible fungus dactylium, containing prochloraz manganese salt and xinjunan acetate as active components, wherein a weight ratio of the prochloraz manganese salt to the xinjunan acetate is 25:1-100:1.

7. The pesticide for preventing and controlling edible fungus dactylium according to claim 6, characterized in that the pathogen of edible fungus dactylium is C. varium.

8. The pesticide for preventing and controlling edible fungus dactylium according to claim 6 or 7, characterized in that the dosage form of the pesticide can be selected from any one of a wettable powder, a water dispersible granule, a suspending agent and an water emulsion.

9. An application of the pesticide according to claim 8 in preventing and controlling edible fungus dactylium.

10. A method for preventing and controlling edible fungus dactylium, characterized in that the edible fungi are spray with the pesticide according to claim 8, wherein the pathogen of ek#123702 fungus dactylium is C. varium.