CN116998632A - Additive and feed for promoting fish growth under saline-fresh water culture condition - Google Patents

Additive and feed for promoting fish growth under saline-fresh water culture condition Download PDF

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Publication number
CN116998632A
CN116998632A CN202310958363.5A CN202310958363A CN116998632A CN 116998632 A CN116998632 A CN 116998632A CN 202310958363 A CN202310958363 A CN 202310958363A CN 116998632 A CN116998632 A CN 116998632A
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China
Prior art keywords
additive
fresh water
growth
feed
fish
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CN202310958363.5A
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Chinese (zh)
Inventor
张晋
刘鼎瑞
杨佳雨
马泽昊
邹振江
李水生
卢丹琪
张勇
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Sun Yat Sen University
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Sun Yat Sen University
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Priority to CN202310958363.5A priority Critical patent/CN116998632A/en
Publication of CN116998632A publication Critical patent/CN116998632A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses an additive for promoting fish growth under the condition of saline-fresh water culture, which comprises the following components: betaine, linoleic acid and vitamin B1; the additive can play a good role in promoting growth of fishes under the condition of saline-fresh water culture, and remarkably improves the growth performance of the fishes. The invention also discloses a feed for promoting the growth of fish under the condition of saline-fresh water culture; the feed additive has good growth promoting effect on important aquatic economic animals such as grass carp, yellow fin sea bream and the like, and has wide development prospect.

Description

Additive and feed for promoting fish growth under saline-fresh water culture condition
Technical Field
The invention belongs to the technical field of aquaculture, and discloses an additive and a feed for promoting fish growth under a saline-fresh water culture condition.
Background
In recent years, with the development of the aquaculture industry, attempts have been made to use brackish water (referring to water salinity of 1-20 ppt) to cultivate freshwater economic species; moreover, researches show that the edible quality of the freshwater economic fish can be improved by using the freshwater for freshwater culture. However, the conventional freshwater economic fishes are cultivated in the salty freshwater, so that the freshwater fishes cannot adapt to the salinity of the water body, the growth is slowed down and the like, and the production benefit is reduced.
In addition, in the prior art, there are reports about the cultivation of brackish fish such as yellow fin sea bream, tilapia, weever and the like by using salty fresh water.
Therefore, in order to improve the growth performance of fishes under the saline-water culture, the invention hopes to provide an additive and a feed for promoting the growth of fishes under the saline-water culture condition, thereby promoting the health and sustainable development of the aquaculture industry in China.
Disclosure of Invention
One of the purposes of the invention is to provide an additive for promoting the growth of fish under the condition of the saline-fresh water culture, which can effectively improve the growth performance of fish under the condition of the saline-fresh water culture.
The second purpose of the invention is to provide a feed for promoting fish growth under the condition of saline-fresh water culture.
The first object of the invention is achieved by the following technical scheme:
an additive for promoting fish growth under the condition of saline-fresh water culture, which comprises the following components: betaine, linoleic acid and vitamin B1.
The fish comprises freshwater fish and guantitous fish; specifically, the freshwater fish can be grass carp, crucian carp, mandarin fish and other fishes, and the guang-salt fish can be yellow fin sea bream, tilapia, weever and other fishes.
Among the components of the additive, linoleic acid has the functions of promoting growth, reducing cholesterol, resisting oxidation, inhibiting fat accumulation and enhancing the immunity of organisms for aquatic animals; vitamin B1 can promote the growth of aquatic animals and has a protective effect on the livers of the aquatic animals; the betaine has the effects of remarkably improving the osmotic pressure regulating capability of aquatic animals, increasing the weight gain rate and improving the survival rate of young fish.
The inventor finds that the additive containing betaine, linoleic acid and vitamin B1 can exert the effect of promoting growth and simultaneously help fish to better adapt to the culture of salty and fresh water; experiments show that the additive can achieve unexpected excellent effects (particularly, the improvement of the weight gain rate and the specific growth rate) on promoting the growth of fishes according to the saline-fresh water culture conditions, and can greatly reduce the death rate of the saline-fresh water culture freshwater fishes, regardless of the freshwater fishes or the wild-salt fishes.
In addition, the betaine, the linoleic acid and the vitamin B1 are all additives with good safety and have no negative influence on fish and the environment.
As a preferable scheme of the invention, the additive comprises 45-55% of betaine, 44.97-54.97% of linoleic acid and 0.02-0.03% of vitamin B1 by mass percent.
More preferably, the additive comprises, in mass percent, 50% betaine, 49.97% linoleic acid and 0.03% vitamin B1.
The preparation process of the additive comprises the following steps: mixing betaine and vitamin B1, pulverizing, sieving with 80 mesh sieve, and mixing with linoleic acid to obtain the additive.
The second object of the invention is achieved by the following technical scheme:
a feed for promoting fish growth under the condition of saline-fresh water culture, which comprises the additive.
Preferably, the additive is added into the feed in an amount of 1.5-2% by mass percent.
More preferably, the additive is added in an amount of 2% by mass.
Compared with the prior art, the invention has the following beneficial effects:
the invention combines betaine, vitamin B1 and linoleic acid, and has obvious effect on improving the growth performance of fish under the condition of saline-fresh water culture through synergistic effect.
The additive disclosed by the invention can be used for improving the survival rate of freshwater fish, and the growth performance of freshwater fish under the condition of saline-water culture is better than that of freshwater fish under the condition of existing freshwater culture; for the wild salt fish, the additive provided by the invention can be used in the feed to greatly improve the growth performance of the wild salt fish under the saline-fresh water culture.
In addition, the additive has the advantages of safety, no toxicity, stability and growth promotion, and has wide development prospect in the current application state in the aquaculture industry.
Detailed Description
In order to make the technical solutions of the present invention more apparent to those skilled in the art, the following examples will be presented. It should be noted that the following embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited to the following embodiments, and any modifications, substitutions, and combinations made without departing from the spirit and principles of the present invention are included in the scope of the present invention.
The starting materials, reagents or apparatus used in the following examples were obtained from conventional commercial sources unless otherwise specified.
Example 1
The embodiment provides a feed for promoting the growth of grass carp under the condition of saline-fresh water culture, which is prepared by the following steps:
(1) Weighing betaine, linoleic acid and vitamin B1 according to the mass ratio of 45:54.97:0.03; mixing and crushing betaine and vitamin B1, sieving with a 80-mesh sieve, and mixing with linoleic acid to obtain an additive for promoting fish growth under the condition of saline-fresh water culture;
(2) The additive is added into the commercial grass carp compound feed according to the mass ratio of 1.5 percent and is uniformly mixed, so that the feed for promoting the growth of grass carp under the condition of saline-fresh water culture is obtained.
Example 2
The embodiment provides a feed for promoting the growth of the oplegnathus fasciatus under the condition of saline-fresh water culture, which is prepared by the following steps:
(1) Weighing betaine, linoleic acid and vitamin B1 according to the mass ratio of 50:49.97:0.03; mixing and crushing betaine and vitamin B1, sieving with a 80-mesh sieve, and mixing with linoleic acid to obtain an additive for promoting fish growth under the condition of saline-fresh water culture;
(2) The additive is added into the commercial compound feed of the yellow-fin sea bream according to the mass ratio of 2 percent and is uniformly mixed, so that the feed for promoting the growth of the yellow-fin sea bream under the condition of saline-fresh water culture is obtained.
Example 3
The embodiment provides a feed for promoting the growth of tilapia under the condition of saline-fresh water culture, which is prepared by the following steps:
(1) Weighing betaine, linoleic acid and vitamin B1 according to the mass ratio of 50:49.97:0.03; mixing and crushing betaine and vitamin B1, sieving with a 80-mesh sieve, and mixing with linoleic acid to obtain an additive for promoting fish growth under the condition of saline-fresh water culture;
(2) The additive is added into the commercial tilapia compound feed according to the mass ratio of 2 percent and is uniformly mixed, so that the feed for promoting the growth of the tilapia under the condition of saline-fresh water culture is obtained.
Example 4
The embodiment provides a feed for promoting the growth of mandarin fish under the condition of saline-fresh water culture, which is prepared by the following steps:
(1) Weighing betaine, linoleic acid and vitamin B1 according to the mass ratio of 45:54.97:0.03; mixing and crushing betaine and vitamin B1, sieving with a 80-mesh sieve, and mixing with linoleic acid to obtain an additive for promoting fish growth under the condition of saline-fresh water culture;
(2) The additive is added into the commercial mandarin fish compound feed according to the mass ratio of 1.5 percent and is uniformly mixed, so that the feed for promoting the growth of the mandarin fish under the condition of saline-fresh water culture is obtained.
Comparative example 1
This example provides a grass carp feed, which differs from example 1 in that the additive of step (1) consists of the following components in the following proportions: 100% linoleic acid, and step (2) is the same.
Comparative example 2
This example provides a feed for oplegnathus fasciatus, which differs from example 2 in that the additive in step (1) consists of the following components in the following proportions: 100% linoleic acid, and step (2) is the same.
Comparative example 3
The present example provides a tilapia feed, which differs from example 3 in that the additive of step (1) consists of the following components in the following proportions: 100% linoleic acid, and step (2) is the same.
Comparative example 4
The embodiment provides a mandarin fish feed, which is different from embodiment 4 in that the additive in step (1) is composed of the following components in proportion: 100% linoleic acid, and step (2) is the same.
Example 5 grass carp farming test
Selecting grass carp with the same physical health and specification, initial weight of 46.14+ -1.66 g and average body length of 13.87+ -0.86 cm, and dividing 540 grass carp into six test groups;
six test groups were cultivated for 8 weeks in different water salinity (0 and 2 ppt) using the grass carp feed of example 1, comparative example 1 and commercial grass carp compound feed, respectively; each set of experiments was repeated 3 times.
The experimental device consisted of 18 cultivation barrels with a diameter of 1.5m, each cultivation barrel having 30 fish.
During the experiment, monitoring the water quality, discharging pollution every day and ensuring that the dissolved oxygen is sufficient and the PH value is proper; the grass carp feed is fed for 2 times a day (8 am and 6 pm), and the daily feeding amount is 3% of the weight of the grass carp.
The results of the statistical growth data after the cultivation are shown in Table 1.
Weight gain (%) = (end average weight-initial average weight)/initial average weight×100%
Specific growth rate (%) = (ln end average weight-ln initial average weight)/cultivation days×100%
Liver mass ratio (%) = liver weight/end mean weight x 100%
Survival (%) = final/initial number x 100%
TABLE 1 cultivation test results of different grass carp feeds
From the results in table 1, it can be seen that: under the condition of saline-fresh water culture, the growth performance of grass carp is reduced by adopting commercial grass carp compound feed for culture; by adopting the grass carp feed of the embodiment 1 for cultivation, the growth performance of the grass carp under the condition of saline-fresh water cultivation is not reduced, even better than that under the condition of fresh water cultivation, and the survival rate of the grass carp under the condition of saline-fresh water cultivation is improved.
In addition, the grass carp feed of example 1 has a significantly better growth promoting effect on grass carp under both fresh water and salty fresh water aquaculture conditions, although the grass carp feed of comparative example 1 also has a certain growth promoting effect, compared with the commercial grass carp compound feed.
The result shows that the combination of the specific vitamin component (vitamin B1), the specific antioxidant (linoleic acid) and the specific osmotic pressure regulator (betaine) can play a more excellent role, and effectively improve the growth performance and the survival rate of the cultured grass carp under the condition of salty and fresh water; in other words, the additive disclosed by the invention can effectively improve the growth performance and survival rate of traditional freshwater economic fish cultured under the condition of salty freshwater.
Example 6 cultivation test of Pagrus major
Selecting the yellow fin sea bream with healthy constitution, consistent specification, initial weight of 2.42+ -0.18 g and average body length of 3.87+ -0.86 cm, and dividing 540 yellow fin sea bream into six test groups.
The six test groups were cultured in different water salinity (4 and 16 ppt) for 8 weeks using the yellow-fin porgy feed of example 2, comparative example 2, and a commercial yellow-fin porgy compound feed, respectively; each set of experiments was repeated 3 times.
The experimental device consisted of 18 cultivation barrels with a diameter of 1.5m, each cultivation barrel having 30 fish.
During the experiment, the water quality is monitored, pollution is discharged every day, the dissolved oxygen is ensured to be sufficient, and the PH value is proper; the feeding is carried out 2 times a day (8 am and 6 pm), and the daily feeding amount is 3% of the weight of the yellow fin sea bream.
The results of the statistical growth data after the cultivation are shown in Table 2.
Weight gain (%) = (end average weight-initial average weight)/initial average weight×100%
Specific growth rate (%) = (ln end average weight-ln initial average weight)/cultivation days×100%
Liver mass ratio (%) = liver weight/end mean weight x 100%
Survival (%) = final/initial number x 100%
TABLE 2 results of cultivation tests of different feeds for yellow fin sea bream
From the results in table 2, it can be seen that: under the condition of saline-water culture, compared with the commercial compound feed for the yellow-fin porgy, the feed for the yellow-fin porgy in the embodiment 2 has excellent effect on improving the growth performance of the yellow-fin porgy. The results show that the combination of the specific vitamin component (vitamin B1), the specific antioxidant (linoleic acid) and the specific osmotic pressure regulator (betaine) can play a more excellent role in promoting growth under the condition of saline-fresh water culture, and the growth performance of the raised yellow-fin sea bream is greatly improved.
Example 7 Tilapia culture test
Selecting tilapia with the same physical health and specification, initial weight of 46.14+ -1.66 g and average body length of 13.87+ -0.86 cm, and dividing 540 tilapia into six test groups;
the tilapia feed of example 3, comparative example 3 and the commercial tilapia compound feed were cultivated in six test groups at different salinity (0 and 16 ppt) for 8 weeks, respectively; each set of experiments was repeated 3 times.
The experimental device consisted of 18 cultivation barrels with a diameter of 1.5m, each cultivation barrel having 30 fish.
During the experiment, the water quality is monitored, pollution is discharged every day, the dissolved oxygen is ensured to be sufficient, and the PH value is proper; the feeding is carried out 2 times a day (8 am and 6 pm), and the daily feeding amount is 3% of the body weight of the tilapia.
The results of the statistical growth data after the cultivation are shown in Table 3.
Weight gain (%) = (end average weight-initial average weight)/initial average weight×100%
Specific growth rate (%) = (ln end average weight-ln initial average weight)/cultivation days×100%
Liver mass ratio (%) = liver weight/end mean weight x 100%
Survival (%) = final/initial number x 100%
TABLE 3 results of cultivation tests of different tilapia feeds
From the results in table 3, it can be seen that: under the condition of saline-fresh water culture, the growth performance of the tilapia is reduced by adopting the commercial tilapia compound feed for culture; the tilapia feed in the embodiment 3 is adopted for cultivation, so that the growth performance of the tilapia under the condition of salty and fresh water cultivation is not reduced, and is even better than that under the condition of fresh water cultivation; the additive provided by the invention can ensure that tilapia has better growth performance under the saline-fresh water culture condition.
The results show that the combination of the specific vitamin component (vitamin B1), the specific antioxidant (linoleic acid) and the specific osmotic pressure regulator (betaine) can play a more excellent role, and the growth performance of the tilapia fed under the condition of salty and fresh water is greatly improved.
Example 8 Mandarin fish culture test
Selecting mandarin fish with the same physical health and specification, initial weight of 44.08+ -11.23 g and average body length of 12.36+ -0.99 cm, and dividing 540 mandarin fish into six test groups;
the six test groups were cultured for 8 weeks in different water salinity (0 and 3 ppt) using the mandarin fish feed of example 4, comparative example 4, and the commercial mandarin fish compound feed, respectively; each set of experiments was repeated 3 times.
The experimental device consisted of 18 cultivation barrels with a diameter of 1.5m, each cultivation barrel having 30 fish.
During the experiment, monitoring the water quality, discharging pollution every day and ensuring that the dissolved oxygen is sufficient and the PH value is proper; the mandarin fish is fed for 2 times daily (8 am and 6 pm), and the daily feeding amount is 3% of the weight of mandarin fish.
The results of the statistical growth data after the cultivation are shown in Table 4.
Weight gain (%) = (end average weight-initial average weight)/initial average weight×100%
Specific growth rate (%) = (ln end average weight-ln initial average weight)/cultivation days×100%
Liver mass ratio (%) = liver weight/end mean weight x 100%
Survival (%) = final/initial number x 100%
TABLE 4 cultivation test results of different Mandarin fish feeds
From the results in table 4, it can be seen that: under the condition of salty and fresh water culture, the growth performance of mandarin fish is reduced by adopting a commercial mandarin fish compound feed for culture; compared with the commercial compound feed for the mandarin fish, when the mandarin fish feed of the embodiment 4 is adopted for cultivation, the growth performance of the mandarin fish under the saline-fresh water cultivation condition is obviously improved, and the growth performance is even better than that of the mandarin fish cultivated under the fresh water cultivation condition by adopting the commercial compound feed; moreover, the mandarin fish feed of the embodiment 4 improves the survival rate of mandarin fish under the condition of saline-fresh water culture.
In addition, compared with the mandarin fish compound feed sold in the market, the mandarin fish feed of the comparative example 4 has a certain growth promoting effect, but the effect is far inferior to that of the mandarin fish feed of the example 4.
The result shows that the combination of the specific vitamin component (vitamin B1), the specific antioxidant (linoleic acid) and the specific osmotic pressure regulator (betaine) can play a more excellent role, and the growth performance and the survival rate of the cultured mandarin fish under the condition of salty and fresh water are effectively improved; in other words, the additive disclosed by the invention can effectively improve the growth performance and survival rate of traditional freshwater economic fish cultured under the condition of salty freshwater.

Claims (6)

1. An additive for promoting fish growth under the condition of saline-fresh water culture, which is characterized by comprising the following components: betaine, linoleic acid and vitamin B1.
2. The additive of claim 1, wherein the fish comprises freshwater fish and guano fish.
3. The additive according to claim 2, characterized by comprising 45-55% betaine, 44.97-54.97% linoleic acid and 0.02-0.03% vitamin B1 by mass percent.
4. The additive according to claim 1, wherein the preparation process is: mixing betaine and vitamin B1, pulverizing, sieving with 80 mesh sieve, and mixing with linoleic acid to obtain the additive.
5. A feed for promoting fish growth under brackish water farming conditions, comprising the additive of any one of claims 1-4.
6. The feed according to claim 5, wherein the additive is added in an amount of 1.5 to 2% by mass.
CN202310958363.5A 2023-08-01 2023-08-01 Additive and feed for promoting fish growth under saline-fresh water culture condition Pending CN116998632A (en)

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CN202310958363.5A CN116998632A (en) 2023-08-01 2023-08-01 Additive and feed for promoting fish growth under saline-fresh water culture condition

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CN116998632A true CN116998632A (en) 2023-11-07

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