CN116965360A - Method for cultivating fairy worms - Google Patents
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- CN116965360A CN116965360A CN202311146389.6A CN202311146389A CN116965360A CN 116965360 A CN116965360 A CN 116965360A CN 202311146389 A CN202311146389 A CN 202311146389A CN 116965360 A CN116965360 A CN 116965360A
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 241000195633 Dunaliella salina Species 0.000 claims description 5
- 108090000526 Papain Proteins 0.000 claims description 5
- 241000206744 Phaeodactylum tricornutum Species 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
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- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 3
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- 229930003427 Vitamin E Natural products 0.000 claims description 3
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- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims description 3
- 229940083466 soybean lecithin Drugs 0.000 claims description 3
- 230000007480 spreading Effects 0.000 claims description 3
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- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 235000019155 vitamin A Nutrition 0.000 claims description 3
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- 235000019154 vitamin C Nutrition 0.000 claims description 3
- 239000011718 vitamin C Substances 0.000 claims description 3
- 235000019166 vitamin D Nutrition 0.000 claims description 3
- 239000011710 vitamin D Substances 0.000 claims description 3
- 150000003710 vitamin D derivatives Chemical class 0.000 claims description 3
- 235000019165 vitamin E Nutrition 0.000 claims description 3
- 229940046009 vitamin E Drugs 0.000 claims description 3
- 239000011709 vitamin E Substances 0.000 claims description 3
- 229940045997 vitamin a Drugs 0.000 claims description 3
- 229940046008 vitamin d Drugs 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 3
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- 230000004083 survival effect Effects 0.000 abstract description 2
- 238000012364 cultivation method Methods 0.000 abstract 1
- 241000238582 Artemia Species 0.000 description 30
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- 241000287828 Gallus gallus Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- 239000000645 desinfectant Substances 0.000 description 2
- 230000005059 dormancy Effects 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241001672739 Artemia salina Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a method for cultivating fairy worms, and relates to the technical field of fairy worm cultivation. The cultivation method comprises the steps of pond preparation, seed egg incubation, inoculation, cultivation, harvesting and the like. The invention enables the water body of the pond to flow through the water pump, enables the growth and propagation effect of the artemia to be better, solves the DO problem, improves the resistance and the survival rate of the artemia through the use of specific feed, remarkably improves the content of EPA and DHA in the artemia and keeps higher DHA/EPA ratio, and can meet the requirements of different seedlings on various nutrients.
Description
Technical Field
The invention relates to the technical field of fairy shrimp culture, in particular to a method for culturing fairy shrimp.
Background
Artemia, artemia and brine shrimp are also called brine shrimp, and are small crustaceans with wide worldwide distribution and high salt tolerance. The artemia salina has the advantages of easy culture, short growth period, wide temperature and salinity application range, rich nutrition and the like, is a good biological live bait, and is widely used by aquatic animal breeders such as coastal fish, shrimp and the like in China in recent years. With current market demands, artemia have been in a state of supply and demand.
However, in the existing pond culture process of artemia, the problems that DO parameters are not in accordance with the standard, the artemia and the artemia eggs are low in recovery ratio and the like easily occur. Meanwhile, the existing fairy shrimp feed can improve the yield of the fairy shrimp, but has limited improvement on the quality of the fairy shrimp.
Therefore, how to solve the problems of insufficient supply of the artemia at present, improve the yield and quality of the artemia, and solve the problems that DO parameters are not in accordance with the standard, the recovery ratio of the artemia and the artemia eggs is low, etc. is one of the technical problems to be solved.
Disclosure of Invention
In order to solve the problems, the invention provides a method for cultivating the artemia, which remarkably improves the yield and quality of the artemia by utilizing a specific cultivation process and feed.
The method for cultivating the fairy shrimp comprises the following steps:
s1, pond preparation:
building stems of a pond for preparing cultivation, so that the area of the pond is 80-120 mu; before the fairy shrimp is planted, thoroughly evacuating the pond, insolating for 2 weeks, cleaning the bottom mud of the pond, then, delivering water to the pond, wherein the water depth is 1-1.5 m, then, spreading lime on the bottom of the pond, and then, building a water pump in the pond, wherein the water pumping amount of the water pump is not less than 0.5% of the total water amount of the pond per hour;
s2, hatching eggs:
preparing an incubator and sterilizing, and incubating eggs after sterilizing;
s3, inoculating:
inoculating the hatched artemia;
s4, culturing:
continuously starting a water pump to keep pool water continuously flowing during the whole cultivation period, and feeding compound feed after the artemia grow;
s5, harvesting:
the method comprises the steps of catching and processing the artemia, collecting the hatched artemia at ordinary times, and collecting the artemia eggs in autumn.
Further, the incubator comprises an incubation barrel which is made of organic glass or plastic, the incubation barrel is a barrel body with a circular barrel shape at the upper end and a funnel-shaped bottom, and a small air pump is arranged on the barrel body.
Further, the incubator disinfection method specifically comprises the following steps: the incubating barrel and the air tube of the small air pump are washed by water, then the wall of the incubating barrel is disinfected by strong hypochlorous acid solution, and after 1-2 h, the disinfectant is washed by clean water.
Further, the insect egg disinfection method specifically comprises the following steps: placing the eggs in a sterilizing barrel, adding 200mg/kg hypochlorous acid solution to sterilize the eggs, continuously inflating the eggs through a small air pump in the sterilizing process, continuously sterilizing for 20min, and collecting the sterilized eggs by using a filter screen and fully leaching the eggs.
Further, the hatching is carried out in a hatching barrel, the salinity of water in the hatching process is 25-30 per mill, the temperature is 25-30 ℃, the oxygen content is more than or equal to 2mg/L, the pH is more than 8, the illumination intensity of the water surface is 2000lx, the worm egg density is 3g/L, and the water is fully stirred by a small air pump in the hatching process.
Further, the salinity of the inoculation environment is more than 7 Baume degrees, the inoculation temperature is 18-28 ℃, the pH is 7.9-8.9, the inoculation density is 100 nauplii/L, the transparency of the bait culture pond brine is lower than 30cm, and the water depth is 30-70cm.
Further, the inoculation takes a period of high water temperature in the evening, which is beneficial for the nauplii to be revived.
Further, chicken manure is used for fermenting fat water in the initial stage of cultivation, the transparency is 10-20 cm, the water body is kept at about 7 Baume degrees during cultivation, the water body is subjected to ovulation at about 28 ℃ under the water temperature condition, the hatching rate of eggs before 8 months is 5-30%, the hatching rate of eggs at the bottom of 8 months is 30-50%, and the hatching rate of non-hatched eggs through overwintering dormancy is 70-90%.
Further, the cultivation period is carried out for algae cultivation, wherein 3 to 7 months are mainly diatoms, and 7 to 10 months are mainly green algae.
Further, the compound feed comprises the following raw materials in parts by weight: 2-5 parts of vitamin E, 2-5 parts of vitamin A, 32-5 parts of vitamin D, 2-5 parts of vitamin C, 10-15 parts of composite algae powder, 5-10 parts of fish oil, 5-10 parts of soybean lecithin and 20-25 parts of corn powder, wherein the preparation method of the composite feed comprises the following steps: mixing the raw materials in proportion, homogenizing, and spray drying to obtain the compound feed.
Further, the preparation method of the composite algae powder comprises the following steps: mixing the pseudo-microalgae, phaeodactylum tricornutum and Dunaliella salina according to the weight ratio of 2:1:1, adding water, adding papain and cellulase, performing ultrasonic enzymolysis at 25 ℃ for 30min at 500W, and spray drying to obtain the composite algae powder.
Further, the addition amount of the papain and the cellulase is 2-10wt% of the mixed solution.
Further, the weight ratio of the total weight of the pseudo-microalgae, the Phaeodactylum tricornutum and the dunaliella salina to the water is 1:5-10.
Further, the method for harvesting the artemia cysts comprises the steps of scraping or using a small dip net at the shore, and fishing eggs floating on the water surface or in suspension water at the downwind position.
Further, the small dip net collecting method specifically comprises the following steps: the artemia cysts blown to the bank side by wind are collected, a square small dip net is adopted, and the mesh density is 40 meshes at the upper part and 90 meshes at the bottom.
Compared with the prior art, the invention has the beneficial technical effects that:
the invention enables the water body of the pond to flow through the water pump, so that the growth and propagation effects of the fairy shrimp are better, and the DO problem is solved. The invention can improve the resistance of the artemia and the survival rate of the artemia by using specific feed, thereby realizing sustainable cultivation of the artemia. Meanwhile, the feed disclosed by the invention can obviously improve the content of EPA and DHA in the artemia and keep a higher DHA/EPA ratio, and can meet the requirements of different seedlings on various nutrients. The method for culturing the prawns by taking the fairy shrimp cultured by the method as the feed can obviously improve the quality of the prawns and has good economic value.
Detailed Description
The technical scheme provided by the invention is further described below by combining with the embodiment.
Example 1
A method for cultivating fairy worms comprises the following steps:
s1, pond preparation:
building stems of a pond for preparing cultivation, so that the area of the pond is 120 mu; thoroughly evacuating the pond before planting the fairy worms, insolating for 2 weeks, cleaning bottom mud of the pond, then delivering water to the pond, wherein the water depth is 1.5m, then spreading lime at the bottom of the pond, and then building a water pump in the pond, wherein the water pumping amount of the water pump is 0.8% of the total water amount of the pond per hour;
s2, hatching eggs:
preparing an incubator, wherein the incubator comprises an incubation barrel made of organic glass, the incubation barrel is a barrel body with a circular barrel upper end and a funnel-shaped bottom, a small air pump is arranged on the barrel body, the incubation barrel and the small air pump are cleaned by water, the air pipes of the small air pump are then disinfected by strong hypochlorous acid solution, and the disinfectant is flushed by clean water after 2 hours; placing eggs in a sterilizing barrel, adding 200mg/kg hypochlorous acid solution to sterilize the eggs, continuously inflating the eggs through a small air pump in the sterilizing process, continuously sterilizing for 20min, collecting the sterilized eggs through a filter screen, fully leaching the eggs, incubating the eggs after sterilizing the eggs, wherein the salinity of water in the incubating process is 25 per mill, the temperature is 30 ℃, the oxygen content is more than or equal to 2mg/L, the pH=8.5, the illumination intensity of the water surface is 2000lx, the egg density is 3g/L, and fully stirring the water through the small air pump in the incubating process;
s3, inoculating:
inoculating the hatched artemia, wherein the salinity of the inoculation environment is 7 Baume degrees, the inoculation temperature is 25 ℃, the pH is 8.5, the inoculation density is 100 nauplii/L, the brine transparency of the bait culture pond is low and is 27cm, the water depth is 70cm, and the inoculation time is high water temperature in the evening;
s4, culturing:
continuously starting a water pump during the whole cultivation period to keep the pool water continuously flowing, feeding composite feed after the artemia grow, wherein the transparency of the fed water body is 20cm;
in the initial stage of cultivation, chicken manure is used for fermenting fat water, the transparency of the water in the fermentation fat water stage is 15cm, the water is kept at 7 Baume degrees during cultivation, the water is cultivated for about 15 days under the water temperature condition of 28 ℃, the hatching rate of eggs before 8 months is 26%, the hatching rate of eggs at the bottom of 8 months is 45%, the hatching rate of eggs which do not hatch through overwintering dormancy is 87%, and algae cultivation is carried out during cultivation, wherein diatoms are the main in 3-7 months, and green algae are the main in 7-10 months;
further, the compound feed comprises the following raw materials in parts by weight: 2 parts of vitamin E, 2 parts of vitamin A, 32 parts of vitamin D, 2 parts of vitamin C, 15 parts of composite algae powder, 5 parts of fish oil, 5 parts of soybean lecithin and 2 parts of corn flour, wherein the preparation method of the composite feed comprises the following steps: mixing the raw materials in proportion, homogenizing, and spray drying to obtain compound feed;
further, the preparation method of the composite algae powder comprises the following steps: mixing the pseudo-microalgae, phaeodactylum tricornutum and Dunaliella salina according to the weight ratio of 2:1:1, adding water, adding papain and cellulase, performing ultrasonic enzymolysis at 25 ℃ for 30min at 500W, and spray drying to obtain composite algae powder;
further, the addition amount of the papain and the cellulase is 5% wt of the mixed solution;
further, the weight ratio of the total weight of the pseudo-microalgae, the Phaeodactylum tricornutum and the Dunaliella salina to the water is 1:10;
s5, harvesting:
the method comprises the steps of catching and processing the artemia, collecting the hatched artemia at ordinary times, and collecting the artemia eggs in autumn, wherein the artemia eggs are scraped and picked up by a small net at the shore, the eggs floating on the water surface or in suspension water are fished up at the leeward position, the artemia eggs blown to the shore by wind are collected, and the square small net is adopted, wherein the mesh density is 40 meshes at the upper part and 90 meshes at the bottom.
Comparative example 1
The difference from example 1 is that: the composite feed is not subjected to enzymolysis in the preparation process.
Comparative example 2
The difference from example 1 is that: the ultrasonic treatment is not carried out in the preparation process of the compound feed.
Comparative example 3
The difference from example 1 is that: fish oil is not added in the preparation process of the compound feed.
Comparative example 4,
the difference from example 1 is that: the compound feed is replaced by the aquaculture shrimp feed.
Test example 1
The results of the measurement of adult fairy shrimp yield, egg yield, DHA and EPA content in examples and comparative examples are as follows:
sequence number | Adult yield (jin/mu) | Egg yield (jin/mu) | DHA and EPA content (%) |
Example 1 | 2527 | 12.1 | 30.4 |
Comparative example 1 | 2293 | 10.6 | 23.0 |
Comparative example 2 | 2406 | 11.3 | 26.2 |
Comparative example 3 | 2055 | 8.8 | 18.9 |
The principles and embodiments of the present invention have been described herein with reference to specific examples, the description of which is intended only to assist in understanding the methods of the present invention and the core ideas thereof; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (5)
1. The method for cultivating the fairy worms is characterized by comprising the following steps:
s1, pond preparation:
building stems of a pond for preparing cultivation, so that the area of the pond is 80-120 mu; before the fairy shrimp is planted, thoroughly evacuating the pond, insolating for 2 weeks, cleaning the bottom mud of the pond, then, delivering water to the pond, wherein the water depth is 1-1.5 m, then, spreading lime on the bottom of the pond, and then, building a water pump in the pond, wherein the water pumping amount of the water pump is not less than 0.5% of the total water amount of the pond per hour;
s2, hatching eggs:
preparing an incubator and sterilizing, and incubating eggs after sterilizing;
s3, inoculating:
inoculating the hatched artemia;
s4, culturing:
continuously starting a water pump to keep pool water continuously flowing during the whole cultivation period, and feeding compound feed after the artemia grow;
the compound feed comprises the following raw materials in parts by weight: 2-5 parts of vitamin E, 2-5 parts of vitamin A, 32-5 parts of vitamin D, 2-5 parts of vitamin C, 10-15 parts of composite algae powder, 5-10 parts of fish oil, 5-10 parts of soybean lecithin and 20-25 parts of corn meal;
the preparation method of the composite algae powder comprises the following steps: mixing the pseudo-microalgae, phaeodactylum tricornutum and Dunaliella salina according to the weight ratio of 2:1:1, adding water, adding papain and cellulase, performing ultrasonic enzymolysis at 25 ℃ for 30min at 500W, and spray drying to obtain composite algae powder;
s5, harvesting:
the artemia are caught and processed, and the hatched artemia are collected at ordinary times.
2. The method for cultivating fairy shrimp according to claim 1, wherein the incubator comprises an incubation barrel made of organic glass or plastic, the incubation barrel is a barrel body with a circular barrel upper end and a funnel-shaped bottom, and a small air pump is arranged on the barrel body.
3. The method for cultivating fairy shrimp according to claim 1, wherein the egg disinfection method specifically comprises the following steps: placing the eggs in a sterilizing barrel, adding 200mg/kg hypochlorous acid solution to sterilize the eggs, continuously inflating the eggs through a small air pump in the sterilizing process, continuously sterilizing for 20min, and collecting the sterilized eggs by using a filter screen and fully leaching the eggs.
4. The method for cultivating fairy shrimp according to claim 1, wherein the hatching is carried out in a hatching barrel, the salinity of water in the hatching process is 25-30 per mill, the temperature is 25-30 ℃, the oxygen content is not less than 2mg/L, the pH is more than 8, the illumination intensity of the water surface is 2000lx, the egg density is 3g/L, and the water is fully stirred by a small air pump in the hatching process.
5. The method for cultivating fairy shrimp according to claim 1, wherein the salinity of the inoculation environment is above 7 baume degrees, the inoculation temperature is 18-28 ℃, the pH is 7.9-8.9, the inoculation density is 100 nauplii/L, the brine transparency of the bait culture pond is lower than 30cm, and the water depth is 30-70cm.
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105766810A (en) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | Method for indoor low-cost high-density sustainable cultivation of artemia |
CN105942025A (en) * | 2016-05-16 | 2016-09-21 | 宁波高新区科博汇科技有限公司 | Nutritional feed promoting growth of eleutheronema tetradactylum as well as preparation and use methods of nutritional feed |
CN110301385A (en) * | 2019-08-16 | 2019-10-08 | 渤海水产股份有限公司 | A method of cultivation fairy shrimp |
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2023
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105766810A (en) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | Method for indoor low-cost high-density sustainable cultivation of artemia |
CN105942025A (en) * | 2016-05-16 | 2016-09-21 | 宁波高新区科博汇科技有限公司 | Nutritional feed promoting growth of eleutheronema tetradactylum as well as preparation and use methods of nutritional feed |
CN110301385A (en) * | 2019-08-16 | 2019-10-08 | 渤海水产股份有限公司 | A method of cultivation fairy shrimp |
Non-Patent Citations (1)
Title |
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丁昌玲;孙修涛;王飞久;梁洲瑞;汪文俊;李涛;: "超声波酶解法提取鼠尾藻多糖", 渔业科学进展, no. 06, 15 December 2011 (2011-12-15), pages 92 - 98 * |
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