CN116868790A - Method for promoting capillary roots of medicinal magnolia - Google Patents
Method for promoting capillary roots of medicinal magnolia Download PDFInfo
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- CN116868790A CN116868790A CN202310915289.9A CN202310915289A CN116868790A CN 116868790 A CN116868790 A CN 116868790A CN 202310915289 A CN202310915289 A CN 202310915289A CN 116868790 A CN116868790 A CN 116868790A
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- 241000218378 Magnolia Species 0.000 title claims abstract description 115
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000001737 promoting effect Effects 0.000 title claims abstract description 18
- 230000012010 growth Effects 0.000 claims abstract description 65
- 239000002689 soil Substances 0.000 claims abstract description 20
- 239000003630 growth substance Substances 0.000 claims abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 10
- 238000003780 insertion Methods 0.000 claims abstract description 9
- 230000037431 insertion Effects 0.000 claims abstract description 9
- 230000003203 everyday effect Effects 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 241000007358 Magnolia biondii Species 0.000 claims description 21
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 21
- 229930192334 Auxin Natural products 0.000 claims description 11
- 239000002363 auxin Substances 0.000 claims description 11
- 201000010099 disease Diseases 0.000 claims description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 11
- 239000004062 cytokinin Substances 0.000 claims description 10
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims description 10
- 241000238631 Hexapoda Species 0.000 claims description 8
- 241000607479 Yersinia pestis Species 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- VVOAZFWZEDHOOU-UHFFFAOYSA-N magnolol Chemical compound OC1=CC=C(CC=C)C=C1C1=CC(CC=C)=CC=C1O VVOAZFWZEDHOOU-UHFFFAOYSA-N 0.000 claims description 6
- 239000005730 Azoxystrobin Substances 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 5
- WFDXOXNFNRHQEC-GHRIWEEISA-N azoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1OC1=CC(OC=2C(=CC=CC=2)C#N)=NC=N1 WFDXOXNFNRHQEC-GHRIWEEISA-N 0.000 claims description 5
- 235000009566 rice Nutrition 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 5
- 241001673966 Magnolia officinalis Species 0.000 claims description 3
- 239000010903 husk Substances 0.000 claims description 2
- 239000013589 supplement Substances 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 description 31
- 230000002786 root growth Effects 0.000 description 16
- IKCXDZCEWZARFL-FOWTUZBSSA-N azoxystrobin acid Chemical compound CO\C=C(\C(O)=O)C1=CC=CC=C1OC1=CC(OC=2C(=CC=CC=2)C#N)=NC=N1 IKCXDZCEWZARFL-FOWTUZBSSA-N 0.000 description 15
- 230000036541 health Effects 0.000 description 15
- 239000003337 fertilizer Substances 0.000 description 13
- 230000008569 process Effects 0.000 description 7
- 238000002791 soaking Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000002131 composite material Substances 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 230000004720 fertilization Effects 0.000 description 6
- 238000005286 illumination Methods 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 229910052698 phosphorus Inorganic materials 0.000 description 6
- 229910052700 potassium Inorganic materials 0.000 description 6
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 6
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 5
- 239000004114 Ammonium polyphosphate Substances 0.000 description 5
- 235000019826 ammonium polyphosphate Nutrition 0.000 description 5
- 229920001276 ammonium polyphosphate Polymers 0.000 description 5
- 239000003617 indole-3-acetic acid Substances 0.000 description 5
- 241000209094 Oryza Species 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000035882 stress Effects 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 229920002752 Konjac Polymers 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 238000005452 bending Methods 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 235000013312 flour Nutrition 0.000 description 3
- 235000010485 konjac Nutrition 0.000 description 3
- 230000011890 leaf development Effects 0.000 description 3
- 239000011777 magnesium Substances 0.000 description 3
- 229910052749 magnesium Inorganic materials 0.000 description 3
- 235000018343 nutrient deficiency Nutrition 0.000 description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229960004889 salicylic acid Drugs 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 2
- -1 alkyl glycoside Chemical class 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 229930182470 glycoside Natural products 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 229940083466 soybean lecithin Drugs 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000008641 drought stress Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Ecology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Forests & Forestry (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for promoting capillary roots of medicinal magnolia, which belongs to the technical field of forestry biology and comprises the following steps: step S1, preparing magnolia capillary roots as propagation materials; s2, cutting capillary roots to a length of about 10-15 cm, and ensuring that each capillary root end is provided with a node; step S3, inserting the cutting medium into a proper cutting medium, wherein the insertion depth is about 1/3 to 1/2 of the root length; step S4, a growth regulator can be properly smeared on the inserted root; step S5, providing different environments for the magnolia capillary roots; step S6, checking every day and changing or maintaining the current environment according to the requirement; and S7, observing and recording rooting condition and growth state of the capillary roots. According to the invention, through the comparison experiments of different soil formulas and environmental factors, the rooting condition and the growth state of the capillary roots can be observed and recorded, so that the rooting and promotion operation of magnolia is facilitated, and the rooting efficiency of the capillary roots and the promotion effect of the capillary roots are greatly improved.
Description
Technical Field
The invention relates to the technical field of forestry biology, in particular to a method for promoting capillary roots of medicinal magnolia.
Background
The function of plant root system can absorb fertilizer and water in soil to supply to the growth and result of branches and leaves. The more developed the root system of the plant is, the more luxuriant the branches and leaves are, and the greater the capability of resisting natural disasters such as strong wind, heavy rain, flushing of flood and the like is; on the other hand, studies have shown that many medicinal plant root-bark contains anticancer medicinal components. Obviously, the method has great significance for promoting the growth of more roots of plants, healthy growth of the plants and providing more root materials for the development of new anticancer drugs.
The magnolia is an old and beautiful plant, is various, can be used as ornamental plants, has medicinal and traditional medical purposes, and is mostly prepared by inserting the root system of the magnolia into a cutting medium and then growing the root system of the magnolia in a natural environment when promoting capillary roots of the magnolia, so that the rooting effect of the magnolia is poor and the rooting quality is low.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a medicinal magnolia capillary root promoting method, which solves the problems of poor rooting effect and low rooting quality of the existing magnolia.
In order to achieve the above purpose, the invention is realized by the following technical scheme: a method for promoting capillary roots of magnolia officinalis for medical use, comprising the following steps:
step S1, preparing magnolia capillary roots as propagation materials;
s2, cutting capillary roots to a length of about 10-15 cm, and ensuring that each capillary root end is provided with a node;
step S3, inserting the cutting medium into a proper cutting medium, wherein the insertion depth is about 1/3 to 1/2 of the root length;
step S4, a growth regulator can be properly smeared on the inserted root;
step S5, providing different environments for the magnolia capillary roots;
step S6, checking every day and changing or maintaining the current environment according to the requirement;
and S7, observing and recording rooting condition and growth state of the capillary roots.
Preferably, the magnolia in step S1 is specifically magnolia biondii, and it is required to ensure that the selected capillary root is free of plant diseases and insect pests.
Preferably, the cutting medium in the step S3 is two or more of sandy soil, red loam, black soil and rice husk.
Preferably, in the step S4, the growth regulator is one or more of auxin, root growth powder, root growth liquid, azoxystrobin and cytokinin.
Preferably, the magnolia in step S1 is specifically 1 year old, 2 years old or 3 years old.
Preferably, in the step S5, the growth environment of the capillary root of magnolia is specifically waterlogged, and then drought and natural environment are adopted.
Preferably, in the step S5, when the capillary roots of magnolia are flooded, the flooding height needs to be ensured to be 2CM above the soil surface.
Preferably, in the step S5, the flooding time period for the capillary roots of magnolia is 1 day, 2 days, 4 days, 7 days and 10 days, and after the water is drained, the drought is maintained until the leaves wilt, and then the flooding treatment is performed again, so that the cycle of the test is repeated for 30 days until the capillary roots are full of the basin.
Preferably, in the step S5, care should be taken that the seedling water replenishment is not given when the magnolia is drought-maintained.
Preferably, in the step S7, when the magnolol capillary root is observed and recorded, one or more of a chart, a photo and a text is specifically used to record the growth condition and rooting condition of the capillary root.
Compared with the prior art, the method for promoting the capillary roots of the medicinal magnolia has the following beneficial effects:
according to the invention, through the comparison experiments of different soil formulas and environmental factors, the rooting condition and the growth state of the capillary roots can be observed and recorded, and the condition that the capillary roots of the magnolia root are most rapidly rooted in which environment and the condition that the capillary roots are most vigorous in which environment can be known, so that the rooting and promotion operation of the magnolia root can be conveniently carried out, and the rooting efficiency and promotion effect of the capillary roots are greatly improved.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Embodiment one:
the embodiment of the invention provides a method for promoting capillary roots of medicinal magnolia, which comprises the following steps of:
step S1, preparing magnolia capillary roots as propagation materials; prior to preparing the capillary roots of magnolia as propagation material, healthy pest-free capillary roots of magnolia are ensured to be selected. Capillary roots are the younger, thinner root systems in magnolia plants, and are generally softer and more productive.
S2, cutting capillary roots to a length of about 10-15 cm, and ensuring that each capillary root end is provided with a node; the nodes are key parts of plant growth and comprise functions of elongation growth and lateral rooting differentiation.
Step S3, inserting the magnolia capillary roots into a proper cutting medium, wherein sandy soil, red loam, black soil or rice hulls and the like can be used, and the insertion depth is about 1/3 to 1/2 of the root length;
step S4, a growth regulator can be properly smeared on the inserted root; optionally, a growth regulator may be applied during root insertion. The growth regulator can promote plant rooting and growth. The common growth regulator comprises auxin, root growth powder, root growth liquid, azoxystrobin, cytokinin and the like, wherein the auxin is naphthalene acetic acid and indoleacetic acid, and the root growth powder and the root growth liquid contain elements such as nitrogen, phosphorus, potassium, calcium, magnesium and the like besides the auxin; the azoxystrobin acid comprises azoxystrobin acid A1 and azoxystrobin acid A3, and the proportion of the azoxystrobin acid A1 to the azoxystrobin acid A3 in the growth of the magnolia lilacina system is 1 to 5 parts per million; the cytokinin comprises salicylic acid and 2, 4-dichlorophenolic acid, and is also required to be fertilized periodically during the rooting period of magnolia in spring, wherein the fertilizer is a compound fertilizer containing N, P, K, and a composite material of ammonium polyphosphate modified konjak flour is also added in the compound fertilizer, the adding amount of the composite material is 0.6% of the mass of the fertilizer, and the specific fertilization time and fertilization amount are adjusted according to the health state of magnolia observed in the step S6. In another embodiment, the growth regulator comprises 4-10 parts of indoleacetic acid, 2-8 parts of naphthylacetic acid, 1-10 parts of soybean lecithin, 1-4 parts of alkyl glycoside, 3-5 parts of ammonium polyphosphate, 1-7 parts of cytokinin, 2-12 parts of humic acid and 50-100 parts of solvent.
Step S5, providing different environments for the magnolia capillary roots; in the propagation process, water flooding treatment is needed, and after water is drained, the drought state is maintained until the leaves wilt, and then water flooding treatment is needed. This process can be repeated, typically for 30 days, until the capillary root is full of the pot.
When the flooding treatment is carried out, the flooding height needs to be paid attention to, and the flooding height is ensured to be about 2cm above the soil surface so as to avoid excessive soaking. In another preferred embodiment, care is taken not to supplement water to the seedlings when the magnolia is drought, stop artificial watering, and build a canopy in rainy days.
Step S6, checking every day and changing or maintaining the current environment according to the requirement; during rooting and growing of magnolia capillary roots, it is important to maintain proper temperature, humidity and lighting conditions. According to observation and needs, the humidity and the illumination intensity of the cutting medium can be timely adjusted to promote the growth of capillary roots. In another preferred embodiment, the current environment is checked daily and changed or maintained as needed; in the rooting and growing process of the capillary roots of magnolia, keeping the temperature at 15-25 ℃ and the humidity according to the size of the tree, watering once every summer and once every three days in spring and autumn, watering once every week in winter, and directly irradiating natural light, wherein the sunshine time is not less than 6 hours.
The judging basis of the health state of magnolia biondii is specifically as follows:
blade condition: leaf of Magnolia biondii was observed. Healthy leaves should appear bright green without obvious leaf spots, withering, or other abnormal symptoms. If the leaves have the problems of wilting, color changing, leaf edge withering and the like, the effect of the magnolia in spring due to the factors of plant diseases and insect pests, nutrient deficiency or environmental pressure can be indicated.
Flower buds and flowers: magnolia biondii is known for its beautiful flowers. The number and development of flower buds and flowers were observed. Healthy magnolia biondii should have a proper amount of flower buds and flowers, and the flowers should be bright in appearance and intact in petals.
Growth conditions: attention is paid to the overall growth condition of magnolia lilacina. Healthy plants have good growth vigor, the stems are firm and upright, the bending degree of the branches is moderate, and no obvious broken branches or dead parts exist. At the same time, the plants were observed for new growth points and healthy leaf development.
Root system state: while the root system is generally not easily directly observable, healthy magnolia biondii should have a healthy root system. The root system should have adequate growth and no obvious signs of decay or disease. If plants are prone to movement or the root system exhibits unhealthy color or texture, it may be necessary to check the health of the root system.
In a simplified embodiment, the observed growth state includes the growth of the leaves and whether the whole plant is lodged or not, and whether the whole color of the plant is normal or not.
And S7, observing and recording rooting condition and growth state of the capillary roots. The recording can be performed by means of charts, pictures, characters and the like. The rooting condition is observed to comprise the number of roots, the length of the roots and the growth condition of the roots. Observing the growth state includes the growth of the leaves and the health of the whole plant. These observations help to assess success rate during reproduction and capillary root health.
The magnolia in step S1 is specifically magnolia biondii, and it is necessary to ensure that the selected capillary root is free of plant diseases and insect pests.
The magnolia in step S1 is specifically 1 year old.
In the step S5, the growth environment of the capillary roots of the magnolia is specifically waterlogged and then drought, the requirement of the growth environment of the magnolia is specifically that the temperature is controlled between 15 ℃ and 30 ℃, the humidity is controlled between 60% and 80%, the illumination time is between 6 hours and 8 hours, and the method needs to be noted that the capillary roots of the magnolia only act on the root system part of the magnolia after waterlogged, and the normal growth environment of the magnolia is not changed.
In step S5, when the magnolia capillary root is flooded, it is necessary to ensure that the flooding height is 2CM above the land. To avoid excessive soaking.
In the step S5, the flooding time of the capillary roots of the magnolia is respectively 1 day, 2 days, 4 days and 7 days, after the water is drained, drought is maintained until the leaves wilt, and then flooding treatment is carried out again, so that the test period is repeated for 30 days until the capillary roots are full of the basin.
In step S5, care should be taken that the seedling water replenishment is not given any more while the magnolia is being maintained drought.
Embodiment two:
in this embodiment, the method for promoting capillary root of magnolia for medical use is further provided by distinguishing the above embodiments, and includes the following steps:
step S1, preparing magnolia capillary roots as propagation materials; prior to preparing the capillary roots of magnolia as propagation material, healthy pest-free capillary roots of magnolia are ensured to be selected. Capillary roots are the younger, thinner root systems in magnolia plants, and are generally softer and more productive.
S2, cutting capillary roots to a length of about 10-15 cm, and ensuring that each capillary root end is provided with a node; the nodes are key parts of plant growth and comprise functions of elongation growth and lateral rooting differentiation.
Step S3, inserting the magnolia capillary roots into a proper cutting medium, wherein sandy soil, red loam, black soil or rice hulls and the like can be used, and the insertion depth is about 1/3 to 1/2 of the root length;
step S4, a growth regulator can be properly smeared on the inserted root; optionally, a growth regulator may be applied during root insertion. The growth regulator can promote plant rooting and growth. The common growth regulator comprises auxin, root growth powder, root growth liquid, azoxystrobin, cytokinin and the like, wherein the auxin is naphthalene acetic acid and indoleacetic acid, and the root growth powder and the root growth liquid contain elements such as nitrogen, phosphorus, potassium, calcium, magnesium and the like besides the auxin; the azoxystrobin acid comprises azoxystrobin acid A1 and azoxystrobin acid A3, and the proportion of the azoxystrobin acid A1 to the azoxystrobin acid A3 in the growth of the magnolia lilacina system is 1 to 5 parts per million; the cytokinin comprises salicylic acid and 2, 4-dichlorophenolic acid, and is also required to be fertilized periodically during the rooting period of magnolia in spring, wherein the fertilizer is a compound fertilizer containing N, P, K, and a composite material of ammonium polyphosphate modified konjak flour is also added in the compound fertilizer, the adding amount of the composite material is 0.6% of the mass of the fertilizer, and the specific fertilization time and fertilization amount are adjusted according to the health state of magnolia observed in the step S6. In a preferred embodiment, suitable growth regulators include 4-10 parts of indoleacetic acid, 2-8 parts of naphthylacetic acid, 1-10 parts of soybean lecithin, 1-4 parts of alkyl glycoside, 3-5 parts of ammonium polyphosphate, 1-7 parts of cytokinin, 2-12 parts of humic acid and 50-100 parts of solvent.
Step S5, providing different environments for the magnolia capillary roots; in the propagation process, water flooding treatment is needed, and after water is drained, the drought state is maintained until the leaves wilt, and then water flooding treatment is needed. This process can be repeated, typically for 30 days, until the capillary root is full of the pot.
When the flooding treatment is carried out, the flooding height needs to be paid attention to, and the flooding height is ensured to be about 2cm above the soil surface so as to avoid excessive soaking.
Step S6, checking every day and changing or maintaining the current environment according to the requirement; during rooting and growing of magnolia capillary roots, it is important to maintain proper temperature, humidity and lighting conditions. According to observation and needs, the humidity and the illumination intensity of the cutting medium can be timely adjusted to promote the growth of capillary roots.
The judging basis of the health state of magnolia biondii is specifically as follows:
blade condition: leaf of Magnolia biondii was observed. Healthy leaves should appear bright green without obvious leaf spots, withering, or other abnormal symptoms. If the leaves have the problems of wilting, color changing, leaf edge withering and the like, the effect of the magnolia in spring due to the factors of plant diseases and insect pests, nutrient deficiency or environmental pressure can be indicated.
Flower buds and flowers: magnolia biondii is known for its beautiful flowers. The number and development of flower buds and flowers were observed. Healthy magnolia biondii should have a proper amount of flower buds and flowers, and the flowers should be bright in appearance and intact in petals.
Growth conditions: attention is paid to the overall growth condition of magnolia lilacina. Healthy plants have good growth vigor, the stems are firm and upright, the bending degree of the branches is moderate, and no obvious broken branches or dead parts exist. At the same time, the plants were observed for new growth points and healthy leaf development.
Root system state: while the root system is generally not easily directly observable, healthy magnolia biondii should have a healthy root system. The root system should have adequate growth and no obvious signs of decay or disease. If plants are prone to movement or the root system exhibits unhealthy color or texture, it may be necessary to check the health of the root system.
And S7, observing and recording rooting condition and growth state of the capillary roots. The recording can be performed by means of charts, pictures, characters and the like. The rooting condition is observed to comprise the number of roots, the length of the roots and the growth condition of the roots. Observing the growth state includes the growth of the leaves and the health of the whole plant. These observations help to assess success rate during reproduction and capillary root health.
The magnolia in step S1 is specifically magnolia biondii, and it is necessary to ensure that the selected capillary root is free of plant diseases and insect pests.
The magnolia in step S1 is specifically 2 years old.
In the step S5, the growth environment of the capillary roots of the magnolia is specifically waterlogged and then drought, the requirement of the growth environment of the magnolia is specifically that the temperature is controlled between 15 ℃ and 30 ℃, the humidity is controlled between 60% and 80%, the illumination time is between 6 hours and 8 hours, and the method needs to be noted that the capillary roots of the magnolia only act on the root system part of the magnolia after waterlogged, and the normal growth environment of the magnolia is not changed.
In step S5, when the magnolia capillary root is flooded, it is necessary to ensure that the flooding height is 2CM above the land. To avoid excessive soaking.
In the step S5, the flooding time of the capillary roots of the magnolia is respectively 1 day, 2 days, 4 days and 7 days, after the water is drained, drought is maintained until the leaves wilt, and then flooding treatment is carried out again, so that the test period is repeated for 30 days until the capillary roots are full of the basin.
In step S5, care should be taken that the seedling water replenishment is not given any more while the magnolia is being maintained drought.
Embodiment III:
in this embodiment, the method for promoting capillary root of magnolia for medical use is further provided by distinguishing the above embodiments, and includes the following steps:
step S1, preparing magnolia capillary roots as propagation materials; prior to preparing the capillary roots of magnolia as propagation material, healthy pest-free capillary roots of magnolia are ensured to be selected. Capillary roots are the younger, thinner root systems in magnolia plants, and are generally softer and more productive.
S2, cutting capillary roots to a length of about 10-15 cm, and ensuring that each capillary root end is provided with a node; the nodes are key parts of plant growth and comprise functions of elongation growth and lateral rooting differentiation.
Step S3, inserting the magnolia capillary roots into a proper cutting medium, wherein sandy soil, red loam, black soil or rice hulls and the like can be used, and the insertion depth is about 1/3 to 1/2 of the root length;
step S4, a growth regulator can be properly smeared on the inserted root; optionally, a growth regulator may be applied during root insertion. The growth regulator can promote plant rooting and growth. The common growth regulator comprises auxin, root growth powder, root growth liquid, azoxystrobin, cytokinin and the like, wherein the auxin is naphthalene acetic acid and indoleacetic acid, and the root growth powder and the root growth liquid contain elements such as nitrogen, phosphorus, potassium, calcium, magnesium and the like besides the auxin; the azoxystrobin acid comprises azoxystrobin acid A1 and azoxystrobin acid A3, and the proportion of the azoxystrobin acid A1 to the azoxystrobin acid A3 in the growth of the magnolia lilacina system is 1 to 5 parts per million; the cytokinin comprises salicylic acid and 2, 4-dichlorophenolic acid, and is also required to be fertilized periodically during the rooting period of magnolia in spring, wherein the fertilizer is a compound fertilizer containing N, P, K, and a composite material of ammonium polyphosphate modified konjak flour is also added in the compound fertilizer, the adding amount of the composite material is 0.6% of the mass of the fertilizer, and the specific fertilization time and fertilization amount are adjusted according to the health state of magnolia observed in the step S6.
Step S5, providing different environments for the magnolia capillary roots; in the propagation process, water flooding treatment is needed, and after water is drained, the drought state is maintained until the leaves wilt, and then water flooding treatment is needed. This process can be repeated, typically for 30 days, until the capillary root is full of the pot.
When the flooding treatment is carried out, the flooding height needs to be paid attention to, and the flooding height is ensured to be about 2cm above the soil surface so as to avoid excessive soaking.
Step S6, checking every day and changing or maintaining the current environment according to the requirement; during rooting and growing of magnolia capillary roots, it is important to maintain proper temperature, humidity and lighting conditions. According to observation and needs, the humidity and the illumination intensity of the cutting medium can be timely adjusted to promote the growth of capillary roots.
The judging basis of the health state of magnolia biondii is specifically as follows:
blade condition: leaf of Magnolia biondii was observed. Healthy leaves should appear bright green without obvious leaf spots, withering, or other abnormal symptoms. If the leaves have the problems of wilting, color changing, leaf edge withering and the like, the effect of the magnolia in spring due to the factors of plant diseases and insect pests, nutrient deficiency or environmental pressure can be indicated.
Flower buds and flowers: magnolia biondii is known for its beautiful flowers. The number and development of flower buds and flowers were observed. Healthy magnolia biondii should have a proper amount of flower buds and flowers, and the flowers should be bright in appearance and intact in petals.
Growth conditions: attention is paid to the overall growth condition of magnolia lilacina. Healthy plants have good growth vigor, the stems are firm and upright, the bending degree of the branches is moderate, and no obvious broken branches or dead parts exist. At the same time, the plants were observed for new growth points and healthy leaf development.
Root system state: while the root system is generally not easily directly observable, healthy magnolia biondii should have a healthy root system. The root system should have adequate growth and no obvious signs of decay or disease. If plants are prone to movement or the root system exhibits unhealthy color or texture, it may be necessary to check the health of the root system.
And S7, observing and recording rooting condition and growth state of the capillary roots. The recording can be performed by means of charts, pictures, characters and the like. The rooting condition is observed to comprise the number of roots, the length of the roots and the growth condition of the roots. Observing the growth state includes the growth of the leaves and the health of the whole plant. These observations help to assess success rate during reproduction and capillary root health.
The magnolia in step S1 is specifically magnolia biondii, and it is necessary to ensure that the selected capillary root is free of plant diseases and insect pests.
The magnolia in step S1 is specifically 3 years old.
In the step S5, the growth environment of the capillary roots of the magnolia is specifically waterlogged and then drought, the requirement of the growth environment of the magnolia is specifically that the temperature is controlled between 15 ℃ and 30 ℃, the humidity is controlled between 60% and 80%, the illumination time is between 6 hours and 8 hours, and the method needs to be noted that the capillary roots of the magnolia only act on the root system part of the magnolia after waterlogged, and the normal growth environment of the magnolia is not changed.
In step S5, when the magnolia capillary root is flooded, it is necessary to ensure that the flooding height is 2CM above the land. To avoid excessive soaking.
In the step S5, the flooding time of the capillary roots of the magnolia is respectively 1 day, 2 days, 4 days and 7 days, after the water is drained, drought is maintained until the leaves wilt, and then flooding treatment is carried out again, so that the test period is repeated for 30 days until the capillary roots are full of the basin.
In step S5, care should be taken that the seedling water replenishment is not given any more while the magnolia is being maintained drought.
Experimental example:
three groups of basically same magnolia are selected for each year to perform various performance tests, a planting experiment is performed by taking magnolia biondii as an example, the detection of the number of capillary roots and the specific surface area of root systems is performed every month, the medicinal magnolia capillary roots of the above examples are respectively adopted as experimental examples, the medicinal magnolia capillary roots of the prior art are adopted as comparative examples, and a comparative experiment is performed. The results are shown in Table 1 below.
Table 1:
according to experimental data of the table, when the root systems of the magnolia which grow for 1 year and 2 years are promoted, good drainage performance of soil needs to be ensured, meanwhile, when flooding is carried out to promote rooting, the condition that the capillary roots of the magnolia are rotten and the growth inhibition of the capillary roots is prevented from being caused by overlong soaking time of the capillary roots of the magnolia, and meanwhile, according to the magnolia capillary roots of different ages, the required soil environment and the flooding time are different, and the table also shows that after the soil is mixed, the growth speed of the capillary roots of the magnolia and the specific surface area of the root system after growth can be greatly improved.
As the root system of the 1-year-old seedling is not fully developed, the resistance to the adversity stress is weaker. The waterlogging and drought stress can cause the root growth limitation of the nursery stock, and the indexes such as root length, root weight and the like can be adversely affected, so that the promotion effect of capillary roots is negatively affected.
The root system of the 2-year-old nursery stock is relatively developed, and the seedling has certain adaptability to adversity stress. However, continuous waterlogging and drought conditions may cause damage to the root system of the nursery stock, limiting growth rate, and thus affecting capillary root development and promotion effects.
The root system of the 3-year-old seedling is relatively mature and robust, and has good adaptability to adversity stress. Although adversity stress may have some negative impact on root growth and metabolism, 3-year old seedlings are more able to maintain some root function in the adversity environment relative to 1-year old and 2-year old seedlings, and thus may have relatively less impact on capillary root promotion effects.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A method for promoting capillary roots of magnolia officinalis for medical use, which is characterized by comprising the following steps:
step S1, preparing magnolia capillary roots as propagation materials;
s2, cutting capillary roots to a length of about 10-15 cm, and ensuring that each capillary root end is provided with a node;
step S3, inserting the cutting medium into a proper cutting medium, wherein the insertion depth is about 1/3 to 1/2 of the root length;
step S4, a growth regulator can be properly smeared on the inserted root;
step S5, providing different environments for the magnolia capillary roots;
step S6, checking every day and changing or maintaining the current environment according to the requirement;
and S7, observing and recording rooting condition and growth state of the capillary roots.
2. The method for promoting capillary roots of magnolia for medical use according to claim 1, wherein the magnolia in step S1 is specifically magnolia biondii, and it is required to ensure that the selected capillary roots are free from diseases and insect pests.
3. The method for promoting capillary roots of magnolia officinalis according to claim 1, wherein the cutting medium in the step S3 is two or more of sandy soil, red loam, black soil and rice husk.
4. The method for promoting capillary roots of magnolia for medical use according to claim 1, wherein in the step S4, the growth regulator is one or more of auxin, root-growing powder, root-growing liquid, azoxystrobin and cytokinin.
5. The method for promoting capillary roots of magnolia for medical use according to claim 1, wherein said magnolia in step S1 is 1 year old, 2 years old or 3 years old.
6. The method according to claim 1, wherein in step S5, the growth environment of the capillary roots of magnolia is specifically waterlogged and then drought and natural.
7. The method according to claim 6, wherein in step S5, the water flooding is performed to ensure that the flooding height is 2CM (CM) above the ground.
8. The method according to claim 6, wherein in step S5, the capillary roots of magnolia are flooded for 1 day, 2 days, 4 days, 7 days and 10 days, respectively, and after the water is drained, drought is maintained until the leaves wilt, and then flooding is performed again, so that the test period is repeated for 30 days until the capillary roots are full of the pot.
9. A method of promoting capillary roots of magnolia for pharmaceutical use according to claim 8, wherein in step S5, care is taken not to supplement water to the seedlings while maintaining drought in magnolia.
10. The method according to claim 1, wherein in step S7, when the magnolol is observed and recorded, one or more of a chart, a photo, and a text is specifically used to record the growth and rooting of the magnolol.
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