CN116806260A - 具有1,3-岩藻糖基转移酶活性的蛋白和含有岩藻糖的糖质的制造方法 - Google Patents

具有1,3-岩藻糖基转移酶活性的蛋白和含有岩藻糖的糖质的制造方法 Download PDF

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Publication number
CN116806260A
CN116806260A CN202280013837.2A CN202280013837A CN116806260A CN 116806260 A CN116806260 A CN 116806260A CN 202280013837 A CN202280013837 A CN 202280013837A CN 116806260 A CN116806260 A CN 116806260A
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Prior art keywords
dna
fucose
amino acid
sequence
seq
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CN202280013837.2A
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Chinese (zh)
Inventor
杉田智惇
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Purimeno Precision Fermentation Japan Co Ltd
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Kyowa Hakko Bio Co Ltd
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Publication of CN116806260A publication Critical patent/CN116806260A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01065—3-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
CN202280013837.2A 2021-02-08 2022-02-08 具有1,3-岩藻糖基转移酶活性的蛋白和含有岩藻糖的糖质的制造方法 Pending CN116806260A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-018483 2021-02-08
JP2021018483 2021-02-08
PCT/JP2022/004974 WO2022168992A1 (ja) 2021-02-08 2022-02-08 １，３－フコシルトランスフェラーゼ活性を有する蛋白質及びフコース含有糖質の製造法

Publications (1)

Publication Number Publication Date
CN116806260A true CN116806260A (zh) 2023-09-26

Family

ID=82742151

Family Applications (1)

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CN202280013837.2A Pending CN116806260A (zh) 2021-02-08 2022-02-08 具有1,3-岩藻糖基转移酶活性的蛋白和含有岩藻糖的糖质的制造方法

Country Status (5)

Country Link
US (1) US12595499B2 (https=)
EP (1) EP4289958A4 (https=)
JP (1) JPWO2022168992A1 (https=)
CN (1) CN116806260A (https=)
WO (1) WO2022168992A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025180259A1 (zh) * 2024-02-26 2025-09-04 恒睿（青岛）生物科技有限公司 岩藻糖基转移酶多肽及基因修饰细胞

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4477740A1 (en) * 2022-02-09 2024-12-18 Kirin Holdings Kabushiki Kaisha Method for producing oligosaccharide having lewis x skeleton
WO2023182527A1 (ja) * 2022-03-25 2023-09-28 キリンホールディングス株式会社 ラクトジフコテトラオース（ｌｄｆｔ）の製造法
WO2025183209A1 (ja) * 2024-03-01 2025-09-04 協和発酵バイオ株式会社 １，３－フコシルトランスフェラーゼ活性を有する蛋白質及びフコース含有糖質の製造法
WO2025239362A1 (ja) * 2024-05-13 2025-11-20 キリンホールディングス株式会社 フコシル化多糖の製造方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57134500A (en) 1981-02-12 1982-08-19 Kyowa Hakko Kogyo Co Ltd Plasmid pcg1
JPS57183799A (en) 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
JPS5835197A (ja) 1981-08-26 1983-03-01 Kyowa Hakko Kogyo Co Ltd プラスミドｐｃｇ２
IL67510A (en) 1981-12-17 1988-08-31 Kyowa Hakko Kogyo Kk Recombinant vector plasmids autonomously replicable in microorganisms belonging to the genus corynebacterium or brevibacterium and process for the production thereof
JPS63233798A (ja) 1986-10-09 1988-09-29 Kyowa Hakko Kogyo Co Ltd ５′−グアニル酸の製造法
JP2545078B2 (ja) 1987-04-06 1996-10-16 協和醗酵工業株式会社 核酸関連物質の製造法
KR100555161B1 (ko) 1996-09-17 2006-11-30 교와 핫꼬 고교 가부시끼가이샤 당뉴클레오티드류및복합당질의제조법
US7569376B2 (en) * 2002-05-03 2009-08-04 Neose Technologies, Inc. Fucosyltransferase fusion protein
EP2944690B1 (en) 2010-10-11 2017-12-20 Jennewein Biotechnologie GmbH Novel fucosyltransferases and their applications
KR101718681B1 (ko) 2014-06-23 2017-03-22 서울대학교산학협력단 가용성 단백질 발현량 및 활성이 증대된 헬리코박터 파일로리 유래 α-1,3 푸코실 전달효소의 유전자와 단백질 및 α-1,3 푸코실올리고당 생산에의 응용
AU2015315110B2 (en) * 2014-09-09 2021-04-08 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides
ES2856749T3 (es) 2016-10-29 2021-09-28 Chr Hansen Hmo Gmbh Proceso para la producción de oligosacáridos fucosilados
JP2022514743A (ja) * 2018-12-18 2022-02-15 インバイオス エン．フェー． ３－フコシルラクトースの産生およびラクトース変換α－１，３－フコシルトランスフェラーゼ酵素
JP2021018483A (ja) 2019-07-17 2021-02-15 キヤノン株式会社 画像処理装置およびその制御方法
CN116848248A (zh) * 2021-02-08 2023-10-03 协和发酵生化株式会社 具有含有岩藻糖的糖质的转运活性的蛋白和含有岩藻糖的糖质的制造方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025180259A1 (zh) * 2024-02-26 2025-09-04 恒睿（青岛）生物科技有限公司 岩藻糖基转移酶多肽及基因修饰细胞

Also Published As

Publication number Publication date
US12595499B2 (en) 2026-04-07
WO2022168992A1 (ja) 2022-08-11
US20240301456A1 (en) 2024-09-12
EP4289958A4 (en) 2025-07-02
JPWO2022168992A1 (https=) 2022-08-11
EP4289958A1 (en) 2023-12-13

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Address after: 1-8-10 Harumi, Chuo City, Tokyo, Japan

Applicant after: Purimeno Precision Fermentation Japan Co., Ltd.

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Address before: Tokyo, Japan

Applicant before: KYOWA HAKKO BIO Co.,Ltd.

Country or region before: Japan