CN116786504A - Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer - Google Patents
Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer Download PDFInfo
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- CN116786504A CN116786504A CN202311095748.XA CN202311095748A CN116786504A CN 116786504 A CN116786504 A CN 116786504A CN 202311095748 A CN202311095748 A CN 202311095748A CN 116786504 A CN116786504 A CN 116786504A
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- outer ring
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- 239000011324 bead Substances 0.000 title claims abstract description 51
- 238000004140 cleaning Methods 0.000 title claims abstract description 43
- 238000003018 immunoassay Methods 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000007788 liquid Substances 0.000 claims description 180
- 238000006243 chemical reaction Methods 0.000 claims description 121
- 239000007924 injection Substances 0.000 claims description 70
- 238000002347 injection Methods 0.000 claims description 70
- 238000003756 stirring Methods 0.000 claims description 32
- 238000005406 washing Methods 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 3
- 125000006850 spacer group Chemical group 0.000 claims 1
- 230000008569 process Effects 0.000 description 8
- 238000000926 separation method Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000011344 liquid material Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/044—Cleaning involving contact with liquid using agitated containers in which the liquid and articles or material are placed
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
Abstract
The invention relates to the technical field of auxiliary cleaning equipment of an immunoassay detector, in particular to a magnetic bead cleaning and separating device and method of a chemiluminescent immunoassay detector.
Description
Technical Field
The invention relates to the technical field of auxiliary cleaning equipment of an immunoassay detector, in particular to a magnetic bead cleaning and separating device and method of a chemiluminescent immunoassay detector.
Background
Chemiluminescent immunoassay is a technique for quantitatively detecting the concentration of an antigen or antibody based on the intensity of luminescence of a luminescent substance using a chemiluminescent or bioluminescent system as an indicator of an antigen-antibody reaction. In an actual chemiluminescent immunoassay analyzer, antigens or antibodies are generally coated on magnetic beads and react with a sample to be detected, so that the labeling of the sample to be detected is completed, therefore, the magnetic bead cleaning and separation naturally becomes an important preparation step of chemiluminescent immunoassay or a step after detection is completed, and the aggregated magnetic beads need to be scattered in the process of cleaning and separation of the magnetic beads so as to increase the cleaning effect of the magnetic beads, and the detection result is ensured not to be interfered by other substances.
In the existing full-automatic chemiluminescence immunoassay analyzer, each reaction cup rotates along with the periphery of a transfer disc, and is fed in sequence for reaction detection, during cleaning, magnetic beads are gathered and absorbed by each reaction cup through a liquid discharge level and a liquid injection level arranged on the transfer disc, and cleaning liquid is added in a dispersing manner, for example, a full-automatic chemiluminescence immunoassay analyzer magnetic bead cleaning device disclosed in China patent document CN103599898B comprises a reaction cup rotating disc, a lifting needle frame, a magnetic bead adsorption device and a mixing device, and a cleaning needle sequentially performs cleaning liquid injection action and waste liquid absorption action on the reaction cup, so that the pipelined cleaning of the magnetic beads is realized, however, as the liquid discharge level and the liquid injection level are not practical, and the transfer disc is required to be stopped when the liquid is discharged, the reaction cup on a vacant interval position, especially in the stopping interval of the transfer disc, does not generate the effect of full cleaning and separation, so that the efficiency of magnetic bead cleaning and separation is not high.
Disclosure of Invention
Therefore, the invention aims to provide a magnetic bead cleaning and separating device and method for a chemiluminescent immunoassay analyzer, which are used for solving the problem of low efficiency of the magnetic bead cleaning and separating of the conventional chemiluminescent immunoassay analyzer.
Based on the above object, the invention provides a magnetic bead cleaning and separating device of a chemiluminescent immunoassay analyzer, comprising a transfer disc, wherein reaction cups containing magnetic beads are arranged at intervals on the periphery of the transfer disc, the side ends of the transfer disc are respectively provided with a liquid discharge level and a liquid injection level, the liquid injection level is provided with a liquid injection component for injecting cleaning liquid into the reaction cups, the liquid discharge level is provided with a liquid discharge component for pumping the cleaning liquid in the reaction cups, and a magnetic piece arranged at the side end of one side of the reaction cups, and when the reaction cups intermittently rotate to the liquid discharge level and the liquid injection level around the axis of the transfer disc, liquid discharge and liquid injection are respectively carried out:
the bottom end of the reaction cup is connected with a cup column, and in an initial state, the cup column is clamped on the transfer disc;
an outer loop is arranged on the outer side of the transfer disc, a sliding rail for sliding connection of a cup column is arranged on the outer loop, two ends of the outer loop are respectively connected to a liquid discharge position and a liquid injection position, a liquid injection stirring component is arranged at one end of the outer loop connected to the liquid injection position and used for stirring a reaction cup filled with liquid on the liquid injection position onto the outer loop, and a liquid discharge stirring component is arranged at one end of the outer loop connected to the liquid discharge position and used for stirring the reaction cup on the outer loop onto the liquid discharge position;
one side of the outer loop is provided with a revolution driving part for driving the reaction cup to slide along the slide rail, and the outer loop is provided with a rotation driving part which drives the reaction cup to rotate when the reaction cup slides along the slide rail.
Preferably, the discharge level, the injection level and the outer ring are provided with a plurality of sets, respectively.
Preferably, a spacing is provided between adjacent drain and fill levels.
Preferably, be equipped with L shape draw-in groove on transporting the dish, L shape draw-in groove includes along the radial section that the radial direction of transporting the dish set up to and along the circumference section that the circumferential direction of transporting the dish set up, under the initial state, cup post joint in circumference section.
Preferably, the side end of the radial section is elastically connected with a baffle, the connection part of the circumferential section and the radial section is rotationally connected with a rotating plate, under the initial state, the baffle pops up and is used for closing one end of the circumferential section, a cup column is propped against the tail end of the rotating plate, when a reaction cup moves out of a transfer disc, the cup column moves out of the circumferential section and the radial section in sequence, the cup column pushes the baffle, and meanwhile, the rotating plate rotates until the baffle is propped against the baffle, so that the baffle is prevented from popping up, and when the cup column moves into an L-shaped clamping groove again, the rotating plate is pushed to rotate until the cup column moves into the circumferential section, the rotating plate is turned away from the baffle, and the baffle pops up again.
Preferably, the outer side end of the baffle is connected with an inclined plate, and when the rotating plate is abutted against the baffle, the tail end of the inclined plate is abutted against the inner side wall of the radial section.
Preferably, the liquid injection stirring assembly comprises a first push plate connected to the side end of the outer ring channel, and when the reaction cup positioned on the liquid injection level rotates along with the transfer disc, the first push plate pushes the reaction cup to move to the outer ring channel.
Preferably, the flowing back is dialled material subassembly and is connected in the second push pedal of outer loop side including rotating, and transport the disk bottom circumference and arrange and be equipped with the dog, and the second push pedal is including being located the upper push plate portion of outer loop one side top and being located the lower push plate portion of transporting disk one side below, and when transporting the disk rotation, the dog promotes lower push plate portion, drives the second push pedal and rotates to make upper push plate portion stir the reaction cup to the flowing back on.
Preferably, the revolution driving part comprises a swivel which is wound on the periphery of the outer loop, clamping cup pieces are arranged on the swivel along the periphery of the swivel, and the outer side ends of the reaction cups are clamped by the clamping cup pieces and used for driving each reaction cup to slide along the sliding rail.
The invention also provides a magnetic bead cleaning and separating method of the chemiluminescent immunoassay analyzer, which comprises the following steps:
under the initial condition, cup post joint is on transporting the dish, and the transportation dish outside is equipped with outer ring road, and the both ends of outer ring road link up respectively in flowing back position, annotate the liquid level, and after the reaction cup annotating the liquid level on the transportation dish annotates the liquid, dial the reaction cup that annotates the liquid level and go up to outer ring road through annotating liquid stirring subassembly, the reaction cup slides along the slide rail to drive the reaction cup rotation through rotation drive unit, when sliding to the other end of outer ring road, rethread flowing back is dialled the reaction cup on the outer ring road and is dialled to the liquid level on the flowing back through flowing back stirring subassembly, carries out flowing back.
The invention has the beneficial effects that: the reaction cup with annotate liquid level on will annotating the liquid level and dial to outer ring road through annotating liquid material subassembly, the reaction cup slides along the slide rail, and drive the reaction cup rotation through rotation drive division, when sliding to outer ring road's the other end, rethread flowing back is dialled the reaction cup on the outer ring road and is dialled to the drainage liquid level, carry out the flowing back, from this, the reaction cup only flowing back and annotate the liquid the process on the transfer dish, the transfer process between flowing back and the notes liquid, all around outer ring road sliding motion, and constantly rotate in sliding, do benefit to magnetic bead and washing liquid fully dispersion and washing in the reaction cup, and even if at the intermittent type of transferring the dish stall and carry out the drainage notes liquid, do not influence the slip rotation of reaction cup on the outer ring road, when the reaction cup rotates in the card cup spare simultaneously, do benefit to the magnetic bead further separation dispersion in the adsorption cup through the magnetic layer, thereby do benefit to higher magnetic bead separation and cleaning efficiency.
Drawings
In order to more clearly illustrate the invention or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only of the invention and that other drawings can be obtained from them without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic view of the structure of the transfer plate and outer ring of the present invention;
FIG. 3 is a schematic view of the structure of the liquid discharge and injection levels of the present invention;
FIG. 4 is an enlarged schematic view of FIG. 3A in accordance with the present invention;
FIG. 5 is a schematic diagram of the structure of the liquid injection stirring assembly of the present invention when starting stirring the reaction cup;
FIG. 6 is a schematic view of the structure of the cup post of the present invention when it is moved into the radial segment;
FIG. 7 is a schematic view of the structure of the reaction cup of the present invention when it is moved to the outer ring;
FIG. 8 is a schematic structural view of the reaction cup of the present invention when the reaction cup is clamped on the clamping piece;
FIG. 9 is a schematic view of the structure of the liquid discharge stirring assembly of the present invention when the reaction cup starts to be stirred;
fig. 10 is an enlarged schematic view of the present invention at B in fig. 9.
Marked in the figure as:
1. a transfer tray; 2. magnetic beads; 3. a reaction cup; 31. a cup column; 311. a transmission gear; 32. a cup body; 33. a cup sleeve; 4. discharging liquid level; 5. filling level; 6. a magnetic member; 7. an outer loop; 71. a slide rail; 8. a liquid injection stirring assembly; 81. a first push plate; 9. a liquid discharge stirring assembly; 91. a second push plate; 911. an upper push plate part; 912. a lower push plate part; 92. a stop block; 10. a revolution driving part; 101. a swivel; 102. a cup clamping piece; 11. a rotation driving unit; 12. an L-shaped clamping groove; 121. a radial segment; 122. a circumferential section; 13. a baffle; 131. a sloping plate; 14. and (5) rotating the plate.
Detailed Description
The present invention will be further described in detail with reference to specific embodiments in order to make the objects, technical solutions and advantages of the present invention more apparent.
It is to be noted that unless otherwise defined, technical or scientific terms used herein should be taken in a general sense as understood by one of ordinary skill in the art to which the present invention belongs. The terms "first," "second," and the like, as used herein, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that elements or items preceding the word are included in the element or item listed after the word and equivalents thereof, but does not exclude other elements or items. The terms "connected" or "connected," and the like, are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", etc. are used merely to indicate relative positional relationships, which may also be changed when the absolute position of the object to be described is changed.
As shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, a magnetic bead cleaning and separating device of a chemiluminescent immunoassay analyzer comprises a transfer disc 1, reaction cups 3 containing magnetic beads 2 are arranged at intervals on the periphery of the transfer disc 1, a liquid discharge level 4 and a liquid injection level 5 are respectively arranged at the side ends of the transfer disc 1, a liquid injection component for injecting cleaning liquid into the reaction cups 3 is arranged on the liquid injection level 5, a liquid discharge component for extracting the cleaning liquid in the reaction cups 3 is arranged on the liquid discharge level 4, a magnetic piece 6 is arranged at one side end of the reaction cups 3, the reaction cups 3 intermittently rotate around the axis of the transfer disc 1 to the liquid discharge level 4 and the liquid injection level 5, liquid discharge and liquid injection are respectively carried out, cup columns 31 are connected at the bottom end of the reaction cups 3, in an initial state, cup columns 31 are clamped on the transfer disc 1, outer ring 7 are arranged on the outer side of the transfer disc 1, slide rails 71 for the cup columns 31 to be connected, two ends of the outer ring 7 are respectively arranged on the liquid discharge level 4 and the liquid injection level 5, the outer ring 7 are connected on one end of the liquid injection level 5, a liquid injection component 8 is arranged on the liquid discharge component 8, and a liquid injection ring 11 is arranged on one end of the liquid injection 5, the liquid injection ring 7 is used for driving the liquid discharge ring 7 to be connected to the liquid discharge ring 3, and the liquid injection ring 7 is arranged on the liquid injection ring 3 and the liquid injection ring 3 is driven to the liquid injection ring 7 and the liquid injection ring 3 and the liquid ring 3 is driven to the liquid ring 3 and the liquid ring 7 is driven to rotate, and the liquid ring 11 is driven to rotate, and the liquid ring 7 is driven to the liquid ring 3 and the liquid ring 7 is respectively.
The invention is based on the structural design of the existing chemiluminescence immunoassay analyzer, which comprises a transfer disc 1, wherein reaction cups 3 containing magnetic beads 2 are arranged at intervals on the periphery of the transfer disc 1, so that each reaction cup 3 rotates along with the periphery of the transfer disc 1, and is sequentially fed for reaction detection, the side end of the transfer disc 1 is respectively provided with a liquid discharge level 4 and a liquid injection level 5, the liquid injection level 5 is provided with a liquid injection component for injecting cleaning liquid into the reaction cups 3, the liquid discharge level 4 is provided with a liquid discharge component for sucking and separating the cleaning liquid in the reaction cups 3, and a magnetic piece 6 arranged at one side end of the reaction cups 3, the magnetic piece 6 can be selected from the existing magnetic components such as permanent magnets, so that when the reaction cups 3 intermittently rotate to the liquid discharge level 4 around the axis of the transfer disc 1, the liquid injection level 5, the magnetic piece 6 is utilized to adsorb the magnetic beads 2 in the reaction cups 3, the liquid discharge component extracts waste in the reaction cups 3, the liquid injection level 5 is utilized to inject the liquid into the reaction cups 3, and the liquid injection component is beneficial to scattering the magnetic beads 2 to increase the cleaning effect of the magnetic beads 2, and the liquid injection component, the liquid injection component and the liquid injection component are driven by the needle 4 to drive the liquid discharge needle 4 to be in a liquid discharge needle lifting structure to be used for cleaning operation.
In particular, the bottom end of the reaction cup 3 is connected with a cup column 31, in an initial state, the cup column 31 is clamped on the transfer disc 1, an outer ring channel 7 is arranged on the outer side of the transfer disc 1, the outer ring channel 7 is independent of the periphery of the transfer disc 1 and does not rotate along with the transfer disc 1, a sliding rail 71 for sliding connection of the cup column 31 is arranged on the outer ring channel 7, two ends of the outer ring channel 7 are respectively connected with a liquid discharge position 4 and a liquid injection level 5, in particular, one end of the outer ring channel 7 connected with the liquid injection level 5 is provided with a liquid injection stirring component 8, one end of the outer ring channel 7 connected with the liquid discharge position 4 is provided with a liquid discharge stirring component 9, one side of the outer ring channel 7 is provided with a revolution driving part 10 for driving the reaction cup 3 to slide along the sliding rail 71, and therefore, after the liquid injection of the reaction cup 3 of the liquid injection level 5 on the transfer disc 1 is finished, the reaction cup 3 of the liquid injection level 5 is stirred onto the outer ring channel 7 through the liquid injection stirring component 8, the reaction cup 3 slides along the slide rail 71 and drives the reaction cup 3 to rotate through the rotation driving part 11, when the reaction cup 3 slides to the other end of the outer ring channel 7, the reaction cup 3 on the outer ring channel 7 is shifted to the liquid discharge position 4 through the liquid discharge shifting component 9 to perform liquid discharge, therefore, the reaction cup 3 only has the liquid discharge and liquid injection processes on the transfer disc 1, the transfer processes between the liquid discharge and the liquid injection do sliding motion around the outer ring channel 7, the quantity of the liquid discharge position 4, the liquid injection level 5, the liquid injection shifting component 8 and the liquid discharge shifting component 9 on the transfer disc 1 is limited, the reaction cup 3 on the idle interval position still slides and rotates, the rotation is not stopped in the sliding process, the magnetic beads 2 and the cleaning liquid are fully dispersed and cleaned in the reaction cup 3, and even if the transfer disc 1 stops rotating to perform the liquid discharge and injection, the sliding rotation of the reaction cup 3 on the outer ring channel 7 is not influenced, thereby facilitating more efficient separation and cleaning of the magnetic beads 2.
In the embodiment of the invention, as shown in fig. 1, 2, 3, 4 and 5, the liquid discharge position 4, the liquid injection position 5 and the outer ring channel 7 are correspondingly provided with a plurality of groups, for example, as shown in fig. 1 and 2, the liquid discharge position 4, the liquid injection position 5 and the outer ring channel 7 are correspondingly provided with three groups, so that the transfer disc 1 rotates for one circle, namely, the reaction cup 3 on the transfer disc 1 is subjected to three-time repeated liquid discharge and injection cleaning processes, thereby being beneficial to separating and cleaning the magnetic beads 2 more efficiently.
In the embodiment of the invention, as shown in fig. 1, 2, 3, 4 and 5, a spacing position is arranged between the adjacent liquid discharge positions 4 and the liquid injection positions 5, so that the influence of the magnetic piece 6 on the reaction cup 3 on the adjacent liquid injection positions 5 is avoided.
In the embodiment of the present invention, as shown in fig. 1, 2, 3, 4 and 5, the transfer disc 1 is provided with an L-shaped clamping groove 12, the L-shaped clamping groove 12 includes a radial section 121 disposed along the radial direction of the transfer disc 1, and a circumferential section 122 disposed along the circumferential direction of the transfer disc 1, in an initial state, the cup column 31 is clamped in the circumferential section 122, and as the reaction cup 3 rotates along with the transfer disc 1, the reaction cup 3 has a centrifugal force towards the radial direction of the transfer disc 1, so that the cup column 31 is not easy to escape from the circumferential section 122.
In the embodiment of the invention, as shown in fig. 1, 2, 3, 4 and 5, the side end of the radial section 121 is elastically connected with a baffle 13, the connection part of the circumferential section 122 and the radial section 121 is rotationally connected with a rotating plate 14, in particular, the baffle 13 is arranged parallel to the radial section 121, an elastic component such as a spring or the like is connected between the side end of the radial section 121, one end of the rotating plate 14 is rotationally connected at the connection inner angle of the circumferential section 122 and the radial section 121, and a torsion spring is arranged at the connection part, in the initial state, as shown in fig. 3 and 4, the baffle 13 pops up to close one end of the circumferential section 122, further the cup column 31 is prevented from freely escaping from the circumferential section 122, the cup column 31 is propped against the tail end of the rotating plate 14, when the reaction cup 3 moves out of the transfer disc 1, as shown in fig. 5, 6, 7 and 8, the cup column 31 moves out along the circumferential section 122 and the radial section 121 in sequence, the cup column 31 gradually pushes the baffle 13 and compresses the elastic component, meanwhile, the rotating plate 14 rotates under the elastic reset action of the torsion spring of the rotating plate 14 until the cup column 31 moves out of the circumferential section 122 completely, the rotating plate 14 rotates to be flush with the circumferential section 122 and is abutted on the baffle 13, the rotating plate 14 rotates to be in place and vertically abuts on the baffle 13, so that the baffle 13 is prevented from being ejected, and when the cup column 31 moves into the L-shaped clamping groove 12 again, the rotating plate 14 is pushed to rotate until the cup column 31 moves into the circumferential section 122, the baffle 13 is rotated away from the baffle 13 again, and the initial state is recovered.
Preferably, as shown in fig. 1, 2, 3, 4 and 5, the outer end of the baffle 13 is connected with a sloping plate 131, and when the rotating plate 14 abuts against the baffle 13, the tail end of the sloping plate 131 abuts against the inner side wall of the radial section 121, so that the cup column 31 can move into the L-shaped clamping groove 12 stably.
In the embodiment of the present invention, as shown in fig. 1, 2, 3, 4 and 5, the liquid injection and material stirring assembly 8 includes a first push plate 81 connected to a side end of the outer ring 7, specifically, the reaction cup 3 includes a cup body 32 and a cup sleeve 33 sleeved on an outer periphery of the cup body 32, the cup body 32 and the cup sleeve 33 are in a cylindrical shape, and when the reaction cup 3 located on the liquid injection level 5 rotates along with the transfer disc 1, the first push plate 81 pushes the reaction cup 3 along a circumferential outer side end surface of the cup sleeve 33 to move toward the outer ring 7.
In the embodiment of the present invention, as shown in fig. 1, 2, 3, 4 and 5, the liquid discharge stirring assembly 9 includes a second push plate 91 rotatably connected to the side end of the outer ring 7, a torsion spring is disposed at the rotational connection, a stop block 92 is circumferentially arranged at the bottom end of the transfer disc 1, the second push plate 91 includes an upper push plate portion 911 located above one side of the outer ring 7 and a lower push plate portion 912 located below one side of the transfer disc 1, as shown in fig. 9 and 10, when the transfer disc 1 rotates, the stop block 92 pushes the lower push plate portion 912 to drive the second push plate 91 to rotate, so that the upper push plate portion 911 stirs the reaction cup 3 onto the liquid discharge position 4.
In the embodiment of the present invention, as shown in fig. 1, 2, 3, 4 and 5, the revolution driving part 10 includes a rotating ring 101 wound around the outer circumference of the outer ring 7, a clamping cup 102 is arranged on the rotating ring 101 along the circumference thereof, specifically, the rotating ring 101 is driven to rotate by a driving motor at a central position and other devices, the clamping cup 102 is in a splayed shape, the clamping cup 102 is clamped with the outer end of the reaction cup 3 and is used for driving each reaction cup 3 to slide along the slide rail 71, a transmission gear 311 is arranged on the cup column 31, the rotation driving part 11 includes a rack arranged at the side end of the slide rail 71, and when the reaction cup 3 slides along the slide rail 71, the reaction cup 3 is driven to rotate by meshing with the rack through the transmission gear 311.
Preferably, the first push plate 81 is provided with an adjustable inner and outer distance, for example, a plate column is arranged at the side end of the outer ring channel 7, the first push plate 81 is slidably connected to the plate column, and the first push plate 81 is adjusted to slide outwards until the reaction cup 3 is moved away from the transfer disc 1, so that the reaction cup 3 on the outer ring channel 7 is not transferred to the outer ring channel 7 any more, and the reaction cup 3 on the outer ring channel 7 is continuously stirred by the liquid discharge stirring assembly 9 to be completely transferred to the transfer disc 1, thereby realizing automatic feed back detection.
Preferably, the magnetic layer is arranged on the periphery of the cup clamping piece 102, and when the reaction cup 3 rotates in the cup clamping piece 102, the magnetic layer is beneficial to further separating and dispersing the magnetic beads 2 in the adsorption cup.
The invention also provides a magnetic bead cleaning and separating method of the chemiluminescent immunoassay analyzer, which comprises the following steps:
in the initial state, cup post 31 joint is on transfer dish 1, transfer the dish 1 outside is equipped with outer lane 7, outer lane 7's both ends link up respectively in flowing back position 4, annotate liquid level 5, transfer dish 1 is gone up annotating liquid level 5's reaction cup 3 and is annotated liquid level 5 and dial to outer lane 7 through annotating liquid stirring subassembly 8 after the end of annotating liquid, reaction cup 3 slides along slide rail 71, and drive reaction cup 3 rotation through rotation drive unit 11, when sliding to the other end of outer lane 7, rethread flowing back stirring subassembly 9 dials reaction cup 3 on the outer lane 7 to flowing back position 4, carry out the flowing back, thereby, reaction cup 3 is on transfer dish 1 only, flowing back and annotate the transfer process between the liquid, all move around outer lane 7 slip, thereby, flowing back position 4 on transfer dish 1, annotate liquid level 5, annotate liquid stirring subassembly 8, flowing back subassembly 9 quantity limited, reaction cup 3 on the vacant interval position still slides and rotates, and do not stop in the magnetic bead that slides, do not stop in the rotation 2 and dispersion, even if the reaction bead is done in the flowing back in the ring 3, and the reaction cup is not fully is washed down to the magnetic bead is moved to the reaction cup 3, even if the reaction bead is not stopped in the rotation of the circulation cup 3, the intermittent type is more completely washed down, the reaction cup is not washed down in the rotation 3, and is more than the magnetic bead is completely washed.
Those of ordinary skill in the art will appreciate that: the discussion of any of the embodiments above is merely exemplary and is not intended to suggest that the scope of the invention (including the claims) is limited to these examples; the technical features of the above embodiments or in the different embodiments may also be combined within the idea of the invention, the steps may be implemented in any order and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.
The present invention is intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omission, modification, equivalent replacement, improvement, etc. of the present invention should be included in the scope of the present invention.
Claims (10)
1. The utility model provides a chemiluminescent immunoassay appearance's magnetic bead washs separator, includes transportation dish (1), transportation dish (1) periphery interval row has reaction cup (3) that contain magnetic bead (2), transportation dish (1) side is equipped with row liquid level (4) respectively and annotates liquid level (5), annotate be equipped with on liquid level (5) be used for to annotate the liquid subassembly of injecting cleaning liquid in reaction cup (3), be equipped with on liquid level (4) and be used for taking out liquid discharging component of washing liquid in reaction cup (3) to and locate magnetic part (6) of reaction cup (3) one side end, reaction cup (3) are around transportation dish (1) axle center intermittent type rotates to liquid discharging position (4) annotate liquid level (5) time, carry out flowing back and annotating liquid respectively, its characterized in that:
the bottom end of the reaction cup (3) is connected with a cup column (31), and in an initial state, the cup column (31) is clamped on the transfer disc (1);
an outer ring channel (7) is arranged on the outer side of the transfer disc (1), a sliding rail (71) for sliding connection of the cup column (31) is arranged on the outer ring channel (7), two ends of the outer ring channel (7) are respectively connected with the liquid discharge level (4) and the liquid injection level (5), a liquid injection stirring component (8) is arranged at one end of the outer ring channel (7) connected with the liquid injection level (5) and used for stirring a reaction cup (3) with the liquid injected on the liquid injection level (5) onto the outer ring channel (7), and a liquid discharge stirring component (9) is arranged at one end of the outer ring channel (7) connected with the liquid discharge level (4) and used for stirring a reaction cup (3) on the outer ring channel (7) onto the liquid discharge level (4);
one side of the outer ring channel (7) is provided with a revolution driving part (10) for driving the reaction cup (3) to slide along the sliding rail (71), the outer ring channel (7) is provided with a rotation driving part (11), and when the reaction cup (3) slides along the sliding rail (71), the reaction cup (3) is driven to rotate through the rotation driving part (11).
2. The magnetic bead washing and separating device of a chemiluminescent immunoassay analyzer according to claim 1, wherein the liquid discharge level (4), the liquid injection level (5) and the outer ring channel (7) are provided with multiple groups respectively.
3. The magnetic bead washing and separating device of a chemiluminescent immunoassay analyzer according to claim 1 wherein a spacer is provided between the adjacent liquid discharge level (4) and the liquid injection level (5).
4. The magnetic bead cleaning and separating device of a chemiluminescent immunoassay analyzer according to claim 1, wherein the transfer plate (1) is provided with an L-shaped clamping groove (12), the L-shaped clamping groove (12) comprises a radial section (121) arranged along the radial direction of the transfer plate (1), and a circumferential section (122) arranged along the circumferential direction of the transfer plate (1), and in an initial state, the cup post (31) is clamped in the circumferential section (122).
5. The magnetic bead cleaning and separating device of a chemiluminescent immunoassay analyzer according to claim 4, wherein a baffle plate (13) is elastically connected to a side end of the radial segment (121), a rotating plate (14) is rotatably connected to a joint of the circumferential segment (122) and the radial segment (121), in an initial state, the baffle plate (13) is ejected for closing one end of the circumferential segment (122), the cup column (31) is pressed against the end of the rotating plate (14), when the reaction cup (3) moves out of the transfer disc (1), the cup column (31) moves out sequentially along the circumferential segment (122) and the radial segment (121), the cup column (31) pushes the baffle plate (13) while the rotating plate (14) rotates until abutting against the baffle plate (13), and when the cup column (31) moves into the L-shaped clamping groove (12) again, the rotating plate (14) is pushed to rotate until the cup column (31) moves into the circumferential segment (12) again, and the baffle plate (13) is ejected again.
6. The magnetic bead cleaning and separating device of a chemiluminescent immunoassay analyzer according to claim 5, wherein an outer side end of the baffle plate (13) is connected with an inclined plate (131), and when the rotary plate (14) abuts against the baffle plate (13), a tail end of the inclined plate (131) abuts against an inner side wall of the radial section (121).
7. The magnetic bead cleaning and separating device of the chemiluminescent immunoassay analyzer according to claim 1, wherein the liquid injection stirring assembly (8) comprises a first push plate (81) connected to the side end of the outer loop (7), and the first push plate (81) pushes the reaction cup (3) to move towards the outer loop (7) when the reaction cup (3) on the liquid injection level (5) rotates along with the transfer disc (1).
8. The magnetic bead cleaning and separating device of the chemiluminescent immunoassay analyzer according to claim 1, wherein the liquid discharge stirring assembly (9) comprises a second push plate (91) rotatably connected to the side end of the outer ring channel (7), a stop block (92) is circumferentially arranged at the bottom end of the transfer disc (1), the second push plate (91) comprises an upper push plate portion (911) located above one side of the outer ring channel (7) and a lower push plate portion (912) located below one side of the transfer disc (1), and when the transfer disc (1) rotates, the stop block (92) pushes the lower push plate portion (912) to drive the second push plate (91) to rotate, so that the upper push plate portion (911) stirs the reaction cup (3) to the liquid discharge position (4).
9. The magnetic bead cleaning and separating device of the chemiluminescent immunoassay analyzer according to claim 1, wherein the revolution driving part (10) comprises a swivel (101) wound around the outer circumference of the outer ring channel (7), the swivel (101) is provided with clamping cup pieces (102) arranged along the circumferential direction, and the clamping cup pieces (102) are clamped with the outer ends of the reaction cups (3) to drive each reaction cup (3) to slide along the sliding rail (71).
10. A magnetic bead washing and separating method using the magnetic bead washing and separating apparatus of a chemiluminescent immunoassay analyzer according to any one of claims 1-9, comprising the steps of:
under the initial condition, cup post (31) joint is on transporting dish (1), transport the dish (1) outside and be equipped with outer lane (7), the both ends of outer lane (7) link up respectively in flowing back position (4), annotate liquid level (5), after annotate liquid end in reaction cup (3) of annotating liquid level (5) on transporting dish (1), dial reaction cup (3) that annotate liquid level (5) and accomplish to outer lane (7) through annotating liquid stirring subassembly (8), reaction cup (3) slide along slide rail (71), and drive reaction cup (3) rotation through rotation drive part (11), when sliding the other end of outer lane (7), rethread flowing back stirring subassembly (9) dials reaction cup (3) on outer lane (7) to flowing back position (4), carry out the flowing back.
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| CN202311095748.XA CN116786504B (en) | 2023-08-29 | 2023-08-29 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
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| CN202311095748.XA CN116786504B (en) | 2023-08-29 | 2023-08-29 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
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Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5242803A (en) * | 1987-07-17 | 1993-09-07 | Martin Marietta Energy Systems, Inc. | Rotor assembly and assay method |
| WO1995008774A2 (en) * | 1993-09-24 | 1995-03-30 | Abbott Laboratories | Automated continuous and random access analytical system and components thereof |
| JP2002350307A (en) * | 2001-05-25 | 2002-12-04 | Hitachi Ltd | Chemical analysis device and its method |
| CN103760373A (en) * | 2014-02-21 | 2014-04-30 | 中国科学院苏州生物医学工程技术研究所 | Cleaning device in fully-automatic chemiluminescence immunity analyzer |
| CN103920675A (en) * | 2014-04-11 | 2014-07-16 | 中国科学院苏州生物医学工程技术研究所 | Magnetic microsphere cleaning device for full-automatic chemiluminescent immunoassay instrument |
| CN203764601U (en) * | 2014-04-11 | 2014-08-13 | 中国科学院苏州生物医学工程技术研究所 | Magnetic microsphere cleaning device used for fully automatic chemiluminescence immunity analyzer |
| CN107262444A (en) * | 2017-06-27 | 2017-10-20 | 苏州长光华医生物医学工程有限公司 | A kind of analyzer magnetic bead cleaning method |
| CN109107966A (en) * | 2018-09-28 | 2019-01-01 | 基蛋生物科技股份有限公司 | Magnetic bead cleaning device and chemical illumination immunity analysis instrument |
| CN210863764U (en) * | 2019-09-29 | 2020-06-26 | 深圳市新产业生物医学工程股份有限公司 | Cleaning module and sample analyzer with same |
| CN112198330A (en) * | 2020-09-27 | 2021-01-08 | 深圳市国赛生物技术有限公司 | Magnetic bead cleaning and separating device |
| CN112858664A (en) * | 2021-01-14 | 2021-05-28 | 烟台信达威医疗科技有限公司 | Cleaning device of chemiluminescence immunoassay analyzer |
| CN114618849A (en) * | 2020-12-11 | 2022-06-14 | 深圳市帝迈生物技术有限公司 | Cleaning device and cleaning method for magnetic separation reaction cup and sample analysis device |
| CN114643248A (en) * | 2020-12-21 | 2022-06-21 | 基蛋生物科技股份有限公司 | Rotating disc type measuring mechanism, cleaning and measuring device and chemiluminescence immunoassay analyzer |
| CN115475809A (en) * | 2021-06-15 | 2022-12-16 | 杭州深度生物科技有限公司 | Novel magnetic separation wash device |
| CN218885938U (en) * | 2022-11-07 | 2023-04-18 | 南京岚煜生物科技有限公司 | Magnetic separation cleaning module |
-
2023
- 2023-08-29 CN CN202311095748.XA patent/CN116786504B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5242803A (en) * | 1987-07-17 | 1993-09-07 | Martin Marietta Energy Systems, Inc. | Rotor assembly and assay method |
| WO1995008774A2 (en) * | 1993-09-24 | 1995-03-30 | Abbott Laboratories | Automated continuous and random access analytical system and components thereof |
| JP2002350307A (en) * | 2001-05-25 | 2002-12-04 | Hitachi Ltd | Chemical analysis device and its method |
| CN103760373A (en) * | 2014-02-21 | 2014-04-30 | 中国科学院苏州生物医学工程技术研究所 | Cleaning device in fully-automatic chemiluminescence immunity analyzer |
| CN103920675A (en) * | 2014-04-11 | 2014-07-16 | 中国科学院苏州生物医学工程技术研究所 | Magnetic microsphere cleaning device for full-automatic chemiluminescent immunoassay instrument |
| CN203764601U (en) * | 2014-04-11 | 2014-08-13 | 中国科学院苏州生物医学工程技术研究所 | Magnetic microsphere cleaning device used for fully automatic chemiluminescence immunity analyzer |
| CN107262444A (en) * | 2017-06-27 | 2017-10-20 | 苏州长光华医生物医学工程有限公司 | A kind of analyzer magnetic bead cleaning method |
| CN109107966A (en) * | 2018-09-28 | 2019-01-01 | 基蛋生物科技股份有限公司 | Magnetic bead cleaning device and chemical illumination immunity analysis instrument |
| CN210863764U (en) * | 2019-09-29 | 2020-06-26 | 深圳市新产业生物医学工程股份有限公司 | Cleaning module and sample analyzer with same |
| CN112198330A (en) * | 2020-09-27 | 2021-01-08 | 深圳市国赛生物技术有限公司 | Magnetic bead cleaning and separating device |
| CN114618849A (en) * | 2020-12-11 | 2022-06-14 | 深圳市帝迈生物技术有限公司 | Cleaning device and cleaning method for magnetic separation reaction cup and sample analysis device |
| CN114643248A (en) * | 2020-12-21 | 2022-06-21 | 基蛋生物科技股份有限公司 | Rotating disc type measuring mechanism, cleaning and measuring device and chemiluminescence immunoassay analyzer |
| CN112858664A (en) * | 2021-01-14 | 2021-05-28 | 烟台信达威医疗科技有限公司 | Cleaning device of chemiluminescence immunoassay analyzer |
| CN115475809A (en) * | 2021-06-15 | 2022-12-16 | 杭州深度生物科技有限公司 | Novel magnetic separation wash device |
| CN218885938U (en) * | 2022-11-07 | 2023-04-18 | 南京岚煜生物科技有限公司 | Magnetic separation cleaning module |
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|---|---|
| CN116786504B (en) | 2023-11-03 |
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