CN109107966A - Magnetic bead cleaning device and chemical illumination immunity analysis instrument - Google Patents
Magnetic bead cleaning device and chemical illumination immunity analysis instrument Download PDFInfo
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- CN109107966A CN109107966A CN201811141653.6A CN201811141653A CN109107966A CN 109107966 A CN109107966 A CN 109107966A CN 201811141653 A CN201811141653 A CN 201811141653A CN 109107966 A CN109107966 A CN 109107966A
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- 238000004140 cleaning Methods 0.000 title claims abstract description 155
- 239000011324 bead Substances 0.000 title claims abstract description 77
- 239000000126 substance Substances 0.000 title claims abstract description 16
- 238000004458 analytical method Methods 0.000 title claims abstract description 15
- 230000036039 immunity Effects 0.000 title claims abstract description 13
- 238000005286 illumination Methods 0.000 title claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 134
- 239000007788 liquid Substances 0.000 claims abstract description 45
- 238000010521 absorption reaction Methods 0.000 claims abstract description 35
- 239000002699 waste material Substances 0.000 claims abstract description 27
- 238000010926 purge Methods 0.000 claims description 41
- 238000001179 sorption measurement Methods 0.000 claims description 15
- 230000007246 mechanism Effects 0.000 claims description 11
- 238000005213 imbibition Methods 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 6
- 230000004044 response Effects 0.000 claims description 5
- 238000003018 immunoassay Methods 0.000 abstract description 4
- 230000003670 easy-to-clean Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 6
- 229910000831 Steel Inorganic materials 0.000 description 5
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 239000010959 steel Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000000007 visual effect Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000008450 motivation Effects 0.000 description 2
- 230000035807 sensation Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 230000029918 bioluminescence Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
- B08B3/022—Cleaning travelling work
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
- B08B9/28—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by splash, spray, or jet application, with or without soaking
- B08B9/30—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by splash, spray, or jet application, with or without soaking and having conveyors
- B08B9/32—Rotating conveyors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention relates to immunoassays to examine field, in particular to a kind of magnetic bead cleaning device and chemical illumination immunity analysis instrument.The magnetic bead cleaning device includes reaction cup disk, cleaning assembly and magnetic absorption component.The cleaning area of reaction cup disk is arranged in cleaning assembly, for successively drawing the waste liquid and addition cleaning solution cleaning reaction cup of each reaction cup by the cleaning area.Magnetic absorption component is connected to reaction cup disk, and during cleaning assembly needs to draw waste liquid, magnetic bead is adsorbed, and component easy to clean draws waste liquid.The magnetic bead cleaning device adsorbs magnetic bead, waste liquid is drawn, cleaning solution fills three function integrated settings in structure, realizes the streamlined cleaning of magnetic bead.
Description
Technical field
The present invention relates to immunoassays to examine field, in particular to a kind of magnetic bead cleaning device and chemiluminescence
Immunity analysis instrument.
Background technique
Chemiluminescence immunoassay technology is to combine chemiluminescence with immune response, it is established that a kind of ultramicron come
Analytical technology.The technology is using chemistry or bio-luminescence system as the indicator of antigen-antibody reaction, according to shiner
Luminous intensity, can be with the concentration of quantitative detection antigen or antibody.This method has been taken into account the high sensitivity of chemiluminescence analysis and has been resisted
The antigen reactive specificity of body.In practical applications, usually antigen or antibody are coated on magnetic bead using technological means, with
Sample reaction to be detected, while another antigen of luminous substance markers or antibody are also reacted with sample to be tested, are just completed to be measured
The label of sample.The purpose of magnetic bead cleaning is exactly that (non-targeted antibody in sample to be tested is anti-for the substance that will be not bonded on magnetic bead
Original has neither part nor lot in the antibody antigen etc. of the luminous substance markers of reaction) it washes away, to guarantee that testing result is not interfered by other substances.
In current existing Full-automatic chemiluminescence immunoassay analysis meter, waste liquid and addition cleaning solution two are drawn in magnetic bead absorption
Function separates in structure, therefore needs auxiliary device to move back and forth reaction cup between the two positions more, and process is cumbersome, stream
Journey is complicated, is unsuitable for automation cleaning.
Summary of the invention
The purpose of the present invention is to provide a kind of magnetic bead cleaning device, solves magnetic bead cleaning and waste liquid in the prior art and draw
When process it is cumbersome, the problem of process complexity.
Another object of the present invention is to provide a kind of chemical illumination immunity analysis instrument, the chemical illumination immunity analysis instrument energy
Enough realize the streamlined cleaning of magnetic bead.
To achieve the goals above, technical solution used in the embodiment of the present invention is as follows:
A kind of magnetic bead cleaning device, comprising: reaction cup disk is provided with multiple holes for placing response cup on reaction cup disk
Position；Reaction cup disk presses period intermittent rotation；Cleaning assembly, cleaning assembly include aspirating needle group and cleaning board group；Clean board group
It is arranged on reaction cup disk, aspirating needle group can pass through cleaning board group and protrude into absorption waste liquid in reaction cup, clean in board group and be arranged
There is cleaning pipe group；And magnetic absorption component, magnetic absorption component include the magnetic absorption portion and driving portion for adsorbing magnetic bead；
Magnetic absorption portion is connected to reaction cup disk；Driving portion is sequentially connected in magnetic absorption portion；Wherein, driving portion is configurable for working as
When aspirating needle group draws the waste liquid in reaction cup, driving magnetic absorption portion is moved to close to reaction cup direction, and magnetic bead is adsorbed
On the side wall of reaction cup.
In preferred embodiments of the present invention, magnetic absorption portion includes magnet adsorption plate, group of magnets and magnetic receiver；Magnet
Group is connected to magnet adsorption plate, and magnet adsorption plate is connected to magnetic receiver, and driving portion is sequentially connected in magnetic receiver；Group of magnets setting exists
It cleans under board group, and is connected to reaction cup disk, the magnetic bead quilt when driving portion drive magnet seat is close to reaction cup, in reaction cup
It is adsorbed on wall of cup.
In preferred embodiments of the present invention, driving portion is spindle motor, and spindle motor is sequentially connected in magnetic receiver.
In preferred embodiments of the present invention, cleaning board group includes clean plate and purge block；Purge block is arranged in clean plate
On, aspirating needle group passes through purge block；Cleaning pipe group includes the first cleaning pipe and the second cleaning pipe, and the setting of the first cleaning pipe is being cleaned
On plate, the second cleaning pipe is arranged in purge block, and the first cleaning pipe is configurable for cleaning reaction cup；Second cleaning pipe is matched
It is set to for cleaning aspirating needle group.
In preferred embodiments of the present invention, purge block includes the first purge block and the second purge block, in the first purge block
It is provided with the second cleaning pipe.
In preferred embodiments of the present invention, the second cleaning pipe includes pipette and spits liquid pipe, pipette and to spit liquid pipe equal
It is connected in purge block.
In preferred embodiments of the present invention, aspirating needle group includes aspirating needle ontology, driving mechanism and linear guide；It drives
Motivation structure is sequentially connected in aspirating needle ontology；Linear guide is connected to reaction cup disk；Driving mechanism is configurable for driving and inhales
Liquid needle ontology moves up and down along linear guide, and waste liquid is drawn in reaction cup to protrude into.
In preferred embodiments of the present invention, aspirating needle group includes the first aspirating needle group and the second aspirating needle group, and first inhales
Liquid needle group and the second aspirating needle group are successively set at the different hole locations of reaction cup disk；First aspirating needle group is matched with the first purge block
It closes；Second aspirating needle group and the second purge block cooperate.
In preferred embodiments of the present invention, reaction cup disk includes turntable body and driving device, and driving device transmission connects
It is connected to turntable body, to drive turntable body by period intermittent rotation；The circumferential direction of turntable body is provided with slot, multiple hole locations
It is successively set in slot.
A kind of chemical illumination immunity analysis instrument, chemical illumination immunity analysis instrument include above-mentioned magnetic bead cleaning device.
The beneficial effects of the present invention are:
A kind of magnetic bead cleaning device provided by the invention, including reaction cup disk, cleaning assembly and magnetic absorption component.Its
In, multiple hole locations for placing response cup are provided on reaction cup disk, reaction cup disk presses period intermittent rotation.Cleaning assembly
Including aspirating needle group and cleaning board group, cleaning board group is arranged on reaction cup disk, and aspirating needle group can pass through cleaning board group and protrude into
Waste liquid is drawn in reaction cup, is cleaned in board group and is provided with cleaning pipe group.Magnetic absorption component includes the magnetism for adsorbing magnetic bead
Adsorption section and driving portion, magnetic absorption portion are connected to reaction cup disk, and driving portion is sequentially connected in magnetic absorption portion.Wherein, it drives
Portion is configurable for before the waste liquid that aspirating needle group is drawn in reaction cup, drives magnetic absorption portion to the side close to reaction cup
To movement, magnetic bead is adsorbed on the side wall of reaction cup.The magnetic bead cleaning device needs to draw the mistake of waste liquid in cleaning assembly
Cheng Zhong adsorbs magnetic bead, and component easy to clean draws waste liquid.The magnetic bead cleaning device adsorbs magnetic bead, waste liquid is drawn, cleaning
Liquid fills three function integrated settings in structure, realizes the streamlined cleaning of magnetic bead.Its structure is simple, control convenience,
Cleaning quality is improved, guarantees the accuracy of subsequent measurement.
A kind of chemical illumination immunity analysis instrument provided by the invention, chemical illumination immunity analysis instrument include that above-mentioned magnetic bead is clear
Cleaning device.By the way that this magnetic bead cleaning device is arranged, which can be realized the streamlined cleaning of magnetic bead.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the structural schematic diagram at the first visual angle of the magnetic bead cleaning device that first embodiment of the invention provides；
Fig. 2 is the structural schematic diagram at the second visual angle of the magnetic bead cleaning device that first embodiment of the invention provides；
Fig. 3 is the structural schematic diagram at the third visual angle for the magnetic bead cleaning device that first embodiment of the invention provides；
Fig. 4 is the structural schematic diagram at the 4th visual angle of the magnetic bead cleaning device that first embodiment of the invention provides.
Icon: 100- magnetic bead cleaning device；110- reaction cup disk；111- turntable body；112- driving device；The hole 113-
Position；114- cover board；120- cleaning assembly；121- aspirating needle group；122- cleans board group；1221- clean plate；1223- first is cleaned
Block；The second purge block of 1224-；123- cleaning pipe group；The first cleaning pipe of 124-；The second cleaning pipe of 125-；130- magnetic absorption group
Part；131- driving portion；133- magnet adsorption plate；134- group of magnets；135- magnetic receiver；140- aspirating needle group；The first imbibition of 141-
Needle group；142- the second aspirating needle group；143- aspirating needle ontology；144- driving mechanism；145- linear guide；146- position-limit mechanism；
150- reaction cup.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented
The component of example can be arranged and be designed with a variety of different configurations.
Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below claimed
The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
In the description of the embodiment of the present invention, it should be noted that the orientation of the instructions such as term " on ", "inner" or position are closed
System for be based on the orientation or positional relationship shown in the drawings or the invention product using when the orientation usually put or position close
System, is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must have
Specific orientation is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.
In the description of the present invention, it is also necessary to which explanation is unless specifically defined or limited otherwise, term " setting ",
" installation ", " connected ", " connection " shall be understood in a broad sense, for example, it may be fixedly connected, may be a detachable connection or one
Connect to body；It can be directly connected, the connection inside two elements can also be can be indirectly connected through an intermediary.
For the ordinary skill in the art, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
First embodiment
Fig. 1-Fig. 4 is please referred to, the present embodiment provides a kind of magnetic bead cleaning devices 100 comprising reaction cup disk 110, cleaning
Component 120 and magnetic absorption component 130.The cleaning area of reaction cup disk 110 is arranged in cleaning assembly 120, for successively inhaling
Take the waste liquid and addition cleaning solution cleaning magnetic bead of each reaction cup 150 by the cleaning area.Magnetic absorption component 130 connects
It is connected to reaction cup disk 110, during cleaning assembly 120 needs to draw waste liquid, magnetic bead is adsorbed, component easy to clean
120 draw waste liquid.Waste liquid is drawn in 100 magnetic bead of magnetic bead cleaning device absorption and addition cleaning solution cleans two functions in structure
Integrated setting realizes the streamlined cleaning of magnetic bead.
Further, reaction cup disk 110 includes turntable body 111 and driving device 112, and driving device 112 is sequentially connected
In turntable body 111, to drive turntable body 111 by period intermittent rotation.
Further, in the present embodiment, above-mentioned driving device 112 includes motor and gear-driven assembly.Motor passes
Dynamic to be connected to gear-driven assembly, gear-driven assembly is sequentially connected in turntable body 111, thus when the motor rotates, turntable
Ontology 111 rotates the spacing of a hole location 113 every a fixed cycle, makes reaction cup under the drive of gear-driven assembly
150 constantly enter cleaning area.
It should be understood that the fixed cycle that above-mentioned turntable body 111 rotates, is configured according to the actual needs.
Further, the circumferential direction along turntable body 111 is provided with slot, and multiple hole locations 113 are successively set in slot.Multiple holes
Position 113 is used for placing response cup 150.
Further, the reaction solution comprising waste liquid and magnetic bead is contained in reaction cup 150.Multiple reaction cups 150 are successively put
It sets in each hole location 113, reaction cup disk 110 passes through cleaning assembly 120 according to period intermittent rotation, each hole location 113
When, reaction cup disk 110 stops the regular hour, and magnetic absorption component 130 adsorbs the magnetic bead in reaction cup 150, cleaning assembly
120 draw the waste liquid in reaction cup 150, cleaning reaction cup 150 and absorption syringe needle.Then reaction cup disk 110 continues to rotate, next
Reaction cup 150 reaches at cleaning assembly 120 in a hole location 113, realizes streamlined cleaning.
In the present embodiment, above-mentioned hole location 113 includes 45.In other alternative-embodiments, the number of hole location 113
It can select to be arranged according to the actual needs.
Further, cleaning assembly 120 includes aspirating needle group 121 and cleaning board group 122.Board group 122 is cleaned to be arranged anti-
It answers on glass disk 110, aspirating needle group 121 can pass through cleaning board group 122 and protrude into absorption waste liquid in reaction cup 150, clean board group 122
On be provided with cleaning pipe group 123.
Further, cover board 114 is provided on reaction cup disk 110.Cleaning board group 122 is connected to cover board 114, and is located at
The edge of reaction cup disk 110.Region where cleaning board group 122 is the cleaning area of reaction cup disk 110.
Further, cleaning board group 122 includes clean plate 1221 and purge block.Purge block is arranged on clean plate 1221,
Aspirating needle group 121 passes through purge block.
Specifically, in the present embodiment, clean plate 1221 includes two.Two clean plates 1221 are successively set on cleaning area
Domain.Purge block includes the first purge block 1223 and the second purge block 1224.On first clean plate 1221, there are two for setting
One purge block 1223；There are two the second purge blocks 1224 for setting on second clean plate 1221.
Further, cleaning pipe group 123 includes the first cleaning pipe 124 and the second cleaning pipe 125, and the first cleaning pipe 124 is set
It sets on clean plate 1221, the second cleaning pipe 125 is arranged in the first purge block 1223, and the first cleaning pipe 124 is configured as using
In cleaning reaction cup 150；Second cleaning pipe 125 is configurable for cleaning aspirating needle group 121.
Specifically, the first cleaning pipe 124 is steel pipe, and steel pipe is connected in clean plate 1221, in use, cleaning solution passes through steel
Pipe is injected in the reaction cup 150 of 1221 lower section of clean plate, is cleaned to the magnetic bead in reaction cup 150.In the present embodiment,
Setting is there are two the first cleaning pipe 124 on first clean plate 1221, be provided on second clean plate 1,221 one first it is clear
Wash pipe 124.
In other embodiments, the number of the first cleaning pipe 124 of setting can also be selected according to the actual needs.
Further, in the present embodiment, it is all provided in two the first purge blocks 1223 on first clean plate 1221
It is equipped with the second cleaning pipe 125.Second is not provided in two the second purge blocks 1224 on second clean plate 1221
Cleaning pipe 125.Second cleaning pipe 125 includes pipette and spits liquid pipe, pipette and spits liquid pipe and is connected to the first purge block
In 1223.Pipette and to spit liquid pipe be bending steel pipe, an imbibition, one is spat liquid, for carrying out clearly to aspirating needle ontology 143
It washes.
In other embodiments, selection is arranged pipette and spits the quantity of liquid pipe according to the actual needs.
Further, in the present embodiment, aspirating needle group 140 includes the first aspirating needle group 141 and the second aspirating needle group
142, the first aspirating needle group 141 and the second aspirating needle group 142 are successively set at the different hole locations 113 of reaction cup disk 110.First
Aspirating needle group 141 and the first purge block 1223 cooperate；Second aspirating needle group 142 and the second purge block 1224 cooperate.
Further, the first aspirating needle group 141 and the second aspirating needle group 142 include two aspirating needle ontologies 143.One
Aspirating needle ontology 143 and a purge block cooperate.
Further, each aspirating needle ontology 143 can relative response cup disk 110 move up and down, thus draw put
Set the waste liquid in the reaction cup 150 of 110 cleaning area of reaction cup disk.
Further, above-mentioned aspirating needle ontology 143 is under the driving of driving mechanism 144, along about 145 linear guide
Movement.
Specifically, in the present embodiment, above-mentioned driving mechanism 144 is lead screw motor.
Further, to prevent the sliding block of linear guide 145 to be detached from linear guide 145, also with limited position mechanism 146.Into
It ensure that one step that aspirating needle group 140 draws the reliability of waste liquid.
Further, magnetic absorption component 130 includes the magnetic absorption portion for adsorbing magnetic bead and driving portion 131.It is magnetic
Adsorption section is connected to reaction cup disk 110, and driving portion 131 is sequentially connected in magnetic absorption portion.Driving portion 131 is configurable for working as
When aspirating needle group 140 draws the waste liquid in reaction cup 150, driving magnetic absorption portion is moved to the direction close to magnetic bead, by magnetic
Pearl is adsorbed in magnetic absorption portion.
Specifically, in the present embodiment, magnetic absorption portion includes magnet adsorption plate 133, group of magnets 134 and magnetic receiver
135.Further, group of magnets 134 is connected to magnet adsorption plate 133, and magnet adsorption plate 133 is connected to magnetic receiver 135, driving portion
131 transmission connections are in magnetic receiver 135.
Further, the setting of group of magnets 134 is under cleaning board group 122, and is connected to reaction cup disk 110, works as driving portion
When 131 drive magnet seats 135 are close to reaction cup disk 110, group of magnets 134 is adsorbed in reaction cup 150, will be in reaction cup 150
Magnetic bead attracted.
Specifically, in the present embodiment, group of magnets 134 includes three pairs of group of magnets 134, amounts to six roots of sensation arc-shaped magnet.The six roots of sensation
Arc-shaped magnet is adsorbed on magnet adsorption plate 133, and magnet adsorption plate 133 is mounted on magnetic receiver 135.
Further, in the present embodiment, driving portion 131 is lead screw motor.Magnetic receiver 135 can be by lead screw motor band
It is dynamic, it is moved forward and backward along the line slide rail being arranged on magnetic receiver 135 to the direction close to or far from reaction cup disk 110.Specifically
Ground, before aspirating needle protrudes into 150 imbibition of reaction cup, magnetic receiver 135 is moved forward, and magnetic bead is adsorbed on 150 arm of reaction cup；
After aspirating needle completes imbibition return, magnetic receiver 135 leaves reaction cup 150, and cleaning steel pipe sprays cleaning solution and cleans to magnetic bead.
Referring to Fig.1, in the present embodiment, three pairs of arc-shaped magnets above-mentioned, the circular arc constituted can be completed at the same time seven instead
The magnetic bead in cup 150 is answered to adsorb work, number consecutively is one to seven counterclockwise by this seven hole locations 113.Four imbibitions
Needle is located at two, four, six, seven, on four hole locations 113.Reaction cup disk 110 is according to cycle time T, in driving device 112
Under driving, a hole location 113 is rotated every time.In one to No. seven hole location 113 there are when reaction cup 150 to be cleaned, in each week
In time phase T, arc-shaped magnet moves forward, close to 150 outer wall of reaction cup under the driving of driving portion 131 along line slide rail.Reaction
Magnetic bead in cup 150 is attracted on 150 inner wall of reaction cup by magnetic fields.Cycle time T soon at the end of, arc
Magnet retracts initial position.
The process that the magnetic bead cleaning device 100 is cleaned is as follows:
Step1: reaction cup disk 110 is rotated by the period, and reaction cup 150 to be cleaned is first under the rotation of reaction cup disk 110
It is introduced into No.1 position.135 driven part 131 of magnetic receiver drives at this time, moves forward along line slide rail, keeps arc-shaped magnet close
Reaction cup 150.Magnetic bead in reaction cup 150 is adsorbed on 150 wall of reaction cup under the influence of magnetic field, after adsorbing a period of time, is driven
Dynamic portion 131 rotates backward, and so that arc-shaped magnet is left reaction cup 150, returns to original position.
Step2: reaction cup disk 110 is rotated by the period, and reaction cup 150 is sent to No. two positions.Driving portion 131 repeats step1
Middle movement, after reaction cup 150 stays for some time No. two positions, aspirating needle ontology 143 above No. two positions, in driving machine
It under structure 144 acts on, is dropped in reaction cup 150 along linear guide 145, siphons away liquid in reaction cup 150, and backed off after random reaction cup
150.During exiting reaction cup 150, aspirating needle ontology 143 can pass through the first purge block 1223, the first purge block 1223
On the second cleaning pipe 125, one is spat liquid, and an imbibition is clean by the remaining waste-liquid cleaning of 143 outer wall of aspirating needle ontology.It inhales
After liquid needle ontology 143 sets back, magnetic receiver 135 is under the effect of driving portion 131, along line slide rail far from reaction cup 150, reaction
Magnetic bead in cup 150 is no longer affected by magnetic fields.At this point, the first cleaning pipe 124 sprays cleaning solution on 150 side wall of reaction cup, beat
The magnetic bead being adsorbed on 150 wall of reaction cup is dissipated, is cleaned.
Step3: after cleaning, reaction cup disk 110 is rotated by the period, and reaction cup 150 is sent to third place, arc-shaped magnet
It repeats to act in step1
Step4: reaction cup disk 110 continues to rotate by the period, comes No. four positions.Each component actuation on No. four positions with
No. two position movements are identical in step2.
Step5: reaction cup disk 110 is rotated by the period, reaction cup 150 is sent to No. five positions, arc-shaped magnet repeats in step1
Movement.
Step6: reaction cup disk 110 is rotated by the period, and reaction cup 150 is sent to No. six position (second be arranged at No. six positions
It is not provided with the second cleaning pipe 125 in purge block 1224, therefore aspirating needle ontology 143 will not be cleaned).On No. six positions
Aspirating needle ontology 143 drops in reaction cup 150 along linear guide 145 under the effect of driving mechanism 144, siphons away reaction cup 150
Interior liquid, and backed off after random reaction cup 150.After aspirating needle ontology 143 sets back, magnetic receiver 135 driving portion 131 effect under,
Along line slide rail far from reaction cup 150, the magnetic bead in reaction cup 150 is no longer affected by magnetic fields.At this point, the first cleaning pipe 124 to
Cleaning solution is sprayed on 150 side wall of reaction cup, the magnetic bead being adsorbed on 150 wall of reaction cup is broken up, is cleaned.
Step7: reaction cup disk 110 is rotated by the period, and reaction cup 150 is sent to No. seven positions.Arc-shaped magnet is in driving portion 131
Under effect, and close to 150 wall of reaction cup, reaction is gathered by the magnetic bead that the first cleaning pipe 124 is sent forth again under magnetic fields
On 150 walls of cup.After reaction cup 150 stays for some time No. seven positions, aspirating needle ontology 143 above No. seven positions is being driven
Under motivation structure 144 acts on, is dropped in reaction cup 150 along linear guide 145, siphon away liquid in reaction cup 150, and backed off after random is anti-
Cup 150 is answered, the cleaning of magnetic bead is completed.
Second embodiment
The present embodiment provides a kind of chemical illumination immunity analysis instrument, which includes first embodiment
The magnetic bead cleaning device of offer.By the way that this magnetic bead cleaning device is arranged, which can be realized magnetic bead
Streamlined cleaning.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.It should also be noted that similar label and letter exist
Similar terms are indicated in following attached drawing, therefore, once being defined in a certain Xiang Yi attached drawing, are then not required in subsequent attached drawing
It is further defined and explained.
Claims (10)
1. a kind of magnetic bead cleaning device characterized by comprising
Reaction cup disk is provided with multiple hole locations for placing response cup on the reaction cup disk；The reaction cup disk presses the period
Intermittent rotation；
Cleaning assembly, the cleaning assembly include aspirating needle group and cleaning board group；The cleaning board group is arranged in the reaction cup
On disk, the aspirating needle group can be protruded into across the cleaning board group draws waste liquid in the reaction cup, in the cleaning board group
It is provided with cleaning pipe group；And
Magnetic absorption component, the magnetic absorption component include the magnetic absorption portion and driving portion for adsorbing magnetic bead；The magnetic
Property adsorption section is connected to the reaction cup disk；The driving portion transmission connection is in the magnetic absorption portion；
Wherein, the driving portion is configurable for before the aspirating needle group draws the waste liquid in the reaction cup, driving
The magnetic absorption portion is moved to the direction close to the reaction cup, and the magnetic bead is adsorbed on to the side wall of the reaction cup
On.
2. magnetic bead cleaning device as described in claim 1, which is characterized in that
The magnetic absorption portion includes magnet adsorption plate, group of magnets and magnetic receiver；
The group of magnets is connected to the magnet adsorption plate, and the magnet adsorption plate is connected to the magnetic receiver, the driving portion
It is sequentially connected in the magnetic receiver；
The group of magnets is arranged under the cleaning board group, and is connected to the reaction cup disk, when the driving portion drives institute
When stating magnetic receiver close to the reaction cup, the magnetic bead in the reaction cup is attracted on wall of cup.
3. magnetic bead cleaning device as claimed in claim 2, which is characterized in that
The driving portion is spindle motor, and the spindle motor transmission connection is in the magnetic receiver.
4. magnetic bead cleaning device as described in claim 1, which is characterized in that
The cleaning board group includes clean plate and purge block；
The purge block is arranged on the clean plate, and the aspirating needle group passes through the purge block；
The cleaning pipe group includes the first cleaning pipe and the second cleaning pipe, and first cleaning pipe is arranged on the clean plate,
Second cleaning pipe is arranged in the purge block, and first cleaning pipe is configurable for cleaning the reaction cup；Institute
The second cleaning pipe is stated to be configurable for cleaning the aspirating needle group.
5. magnetic bead cleaning device as claimed in claim 4, which is characterized in that
The purge block includes the first purge block and the second purge block,
Second cleaning pipe is provided in first purge block.
6. magnetic bead cleaning device as claimed in claim 5, which is characterized in that
Second cleaning pipe includes pipette and spits liquid pipe, and the pipette and the liquid pipe of spitting are connected to the purge block
It is interior.
7. magnetic bead cleaning device as described in claim 1, which is characterized in that
The aspirating needle group includes aspirating needle ontology, driving mechanism and linear guide；
The driving mechanism transmission connection is in the aspirating needle ontology；The linear guide is connected to the reaction cup disk；
The driving mechanism is configurable for that the aspirating needle ontology is driven to move up and down along the linear guide, to protrude into
It states and draws waste liquid in reaction cup.
8. magnetic bead cleaning device as claimed in claim 5, which is characterized in that
The aspirating needle group includes the first aspirating needle group and the second aspirating needle group, the first aspirating needle group and second imbibition
Needle group is successively set at the different hole locations of the reaction cup disk；
The first aspirating needle group and first purge block cooperate；The second aspirating needle group is matched with second purge block
It closes.
9. such as the described in any item magnetic bead cleaning devices of claim 1-8, which is characterized in that
The reaction cup disk includes turntable body and driving device, the driving device transmission connection in the turntable body, with
Drive the turntable body by period intermittent rotation；The circumferential direction of the turntable body is provided with slot, multiple hole locations according to
It is secondary to be arranged in the slot.
10. a kind of chemical illumination immunity analysis instrument, which is characterized in that the chemical illumination immunity analysis instrument includes such as claim
The described in any item magnetic bead cleaning devices of 1-9.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811141653.6A CN109107966A (en) | 2018-09-28 | 2018-09-28 | Magnetic bead cleaning device and chemical illumination immunity analysis instrument |
| PCT/CN2019/091424 WO2020062946A1 (en) | 2018-09-28 | 2019-06-14 | Magnetic bead cleaning device and chemiluminescence immunoassay analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811141653.6A CN109107966A (en) | 2018-09-28 | 2018-09-28 | Magnetic bead cleaning device and chemical illumination immunity analysis instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109107966A true CN109107966A (en) | 2019-01-01 |
Family
ID=64857187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811141653.6A Pending CN109107966A (en) | 2018-09-28 | 2018-09-28 | Magnetic bead cleaning device and chemical illumination immunity analysis instrument |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN109107966A (en) |
| WO (1) | WO2020062946A1 (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110508548A (en) * | 2019-09-19 | 2019-11-29 | 南京迪格诺斯生物技术有限公司 | A kind of developing room adsorption system of chemiluminescent analyzer |
| CN110672835A (en) * | 2019-09-26 | 2020-01-10 | 合肥运涛光电科技有限公司 | Magnetic separation mechanism for chemiluminescence immunoassay system |
| WO2020062946A1 (en) * | 2018-09-28 | 2020-04-02 | 基蛋生物科技股份有限公司 | Magnetic bead cleaning device and chemiluminescence immunoassay analyzer |
| CN111912975A (en) * | 2019-05-09 | 2020-11-10 | 深圳华迈兴微医疗科技有限公司 | Magnetic separation device and chemiluminescence immunoassay analyzer |
| CN114354910A (en) * | 2021-12-13 | 2022-04-15 | 杭州睿丽科技有限公司 | Multichannel chemiluminescence immunoassay analyzer |
| CN115446032A (en) * | 2022-09-15 | 2022-12-09 | 长沙海柯生物科技有限公司 | Liquid adding cleaning structure and liquid adding cleaning method for liquid sucking needle of immunoassay analyzer |
| CN116786504A (en) * | 2023-08-29 | 2023-09-22 | 江苏三联生物工程股份有限公司 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102279275A (en) * | 2011-06-22 | 2011-12-14 | 深圳市国赛生物技术有限公司 | Integrated apparatus for magnetic stirring, cleaning and separation |
| ES2453441A1 (en) * | 2012-09-05 | 2014-04-07 | Metalquimia, Sa | Method and machine for cleaning needles for injecting fluids into meat products |
| CN203737677U (en) * | 2014-02-24 | 2014-07-30 | 湖南康博生物科技有限公司 | Magnetic bead swimming motility washing device for chemical luminescence immunity analyzer |
| CN205393062U (en) * | 2016-02-24 | 2016-07-27 | 南京诺尔曼生物技术有限公司 | Be applied to chemiluminiscence test's combination and wash needle |
| CN105891113A (en) * | 2014-12-16 | 2016-08-24 | 合肥运涛光电科技有限公司 | Reaction vessel for luminescence tester and cleaning and separating mechanism thereof |
| CN106269753A (en) * | 2016-08-31 | 2017-01-04 | 四川迈克生物医疗电子有限公司 | Reaction cup cleans device and sample analyser |
| CN206450696U (en) * | 2017-01-17 | 2017-08-29 | 江苏柯伦迪医疗技术有限公司 | Automatic clinical chemistry analyzer with air jet system |
| CN206470085U (en) * | 2016-12-22 | 2017-09-05 | 深圳开立生物医疗科技股份有限公司 | A kind of sampling needle flusher and analyzer |
| CN208879198U (en) * | 2018-09-28 | 2019-05-21 | 基蛋生物科技股份有限公司 | Magnetic bead cleaning device and chemical illumination immunity analysis instrument |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001091521A (en) * | 1999-09-21 | 2001-04-06 | Olympus Optical Co Ltd | Autoanalyzer |
| US8795609B2 (en) * | 2007-02-08 | 2014-08-05 | Biokit, S.A. | Magnetic particle washing station |
| JP5507432B2 (en) * | 2010-12-14 | 2014-05-28 | シスメックス株式会社 | Analysis apparatus and analysis method |
| CN203426088U (en) * | 2013-08-22 | 2014-02-12 | 钟伟明 | Magnetic bead washing device |
| CN203432982U (en) * | 2013-09-10 | 2014-02-12 | 博奥赛斯（天津）生物科技有限公司 | Magnetic particle cleaning and separating device of chemiluminescent immunoassay analyzer |
| CN103599898B (en) * | 2013-10-29 | 2015-11-25 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immunoassay analysis meter magnetic bead cleaning device |
| CN109107966A (en) * | 2018-09-28 | 2019-01-01 | 基蛋生物科技股份有限公司 | Magnetic bead cleaning device and chemical illumination immunity analysis instrument |
-
2018
- 2018-09-28 CN CN201811141653.6A patent/CN109107966A/en active Pending
-
2019
- 2019-06-14 WO PCT/CN2019/091424 patent/WO2020062946A1/en active Application Filing
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102279275A (en) * | 2011-06-22 | 2011-12-14 | 深圳市国赛生物技术有限公司 | Integrated apparatus for magnetic stirring, cleaning and separation |
| ES2453441A1 (en) * | 2012-09-05 | 2014-04-07 | Metalquimia, Sa | Method and machine for cleaning needles for injecting fluids into meat products |
| CN203737677U (en) * | 2014-02-24 | 2014-07-30 | 湖南康博生物科技有限公司 | Magnetic bead swimming motility washing device for chemical luminescence immunity analyzer |
| CN105891113A (en) * | 2014-12-16 | 2016-08-24 | 合肥运涛光电科技有限公司 | Reaction vessel for luminescence tester and cleaning and separating mechanism thereof |
| CN205393062U (en) * | 2016-02-24 | 2016-07-27 | 南京诺尔曼生物技术有限公司 | Be applied to chemiluminiscence test's combination and wash needle |
| CN106269753A (en) * | 2016-08-31 | 2017-01-04 | 四川迈克生物医疗电子有限公司 | Reaction cup cleans device and sample analyser |
| CN206470085U (en) * | 2016-12-22 | 2017-09-05 | 深圳开立生物医疗科技股份有限公司 | A kind of sampling needle flusher and analyzer |
| CN206450696U (en) * | 2017-01-17 | 2017-08-29 | 江苏柯伦迪医疗技术有限公司 | Automatic clinical chemistry analyzer with air jet system |
| CN208879198U (en) * | 2018-09-28 | 2019-05-21 | 基蛋生物科技股份有限公司 | Magnetic bead cleaning device and chemical illumination immunity analysis instrument |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020062946A1 (en) * | 2018-09-28 | 2020-04-02 | 基蛋生物科技股份有限公司 | Magnetic bead cleaning device and chemiluminescence immunoassay analyzer |
| CN111912975A (en) * | 2019-05-09 | 2020-11-10 | 深圳华迈兴微医疗科技有限公司 | Magnetic separation device and chemiluminescence immunoassay analyzer |
| CN110508548A (en) * | 2019-09-19 | 2019-11-29 | 南京迪格诺斯生物技术有限公司 | A kind of developing room adsorption system of chemiluminescent analyzer |
| CN110672835A (en) * | 2019-09-26 | 2020-01-10 | 合肥运涛光电科技有限公司 | Magnetic separation mechanism for chemiluminescence immunoassay system |
| CN114354910A (en) * | 2021-12-13 | 2022-04-15 | 杭州睿丽科技有限公司 | Multichannel chemiluminescence immunoassay analyzer |
| CN114354910B (en) * | 2021-12-13 | 2023-08-11 | 杭州睿丽科技有限公司 | Multichannel chemiluminescence immunity analyzer |
| CN115446032A (en) * | 2022-09-15 | 2022-12-09 | 长沙海柯生物科技有限公司 | Liquid adding cleaning structure and liquid adding cleaning method for liquid sucking needle of immunoassay analyzer |
| CN115446032B (en) * | 2022-09-15 | 2023-11-10 | 长沙海柯生物科技有限公司 | Liquid adding and cleaning structure and liquid adding and cleaning method for liquid suction needle of immunity analyzer |
| CN116786504A (en) * | 2023-08-29 | 2023-09-22 | 江苏三联生物工程股份有限公司 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
| CN116786504B (en) * | 2023-08-29 | 2023-11-03 | 江苏三联生物工程股份有限公司 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
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