CN111912975A - Magnetic separation device and chemiluminescence immunoassay analyzer - Google Patents

Magnetic separation device and chemiluminescence immunoassay analyzer Download PDF

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Publication number
CN111912975A
CN111912975A CN201910386204.6A CN201910386204A CN111912975A CN 111912975 A CN111912975 A CN 111912975A CN 201910386204 A CN201910386204 A CN 201910386204A CN 111912975 A CN111912975 A CN 111912975A
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China
Prior art keywords
sliding
rod
sliding sleeve
magnetic
sliding rod
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CN201910386204.6A
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Chinese (zh)
Inventor
李泉
张明星
姜润华
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Shenzhen Huamaixingwei Medical Technology Co ltd
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Shenzhen Huamaixingwei Medical Technology Co ltd
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Priority to CN201910386204.6A priority Critical patent/CN111912975A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302Apparatus specially adapted for immunological test procedures

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  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
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  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The invention belongs to the technical field of chemical analysis devices, and particularly relates to a magnetic separation device and a chemiluminescence immunoassay analyzer. The magnetic separation device comprises: a sliding sleeve; the sliding rod is sleeved in the sliding sleeve; a push rod for pushing the slide rod part to extend out of the sliding sleeve; the locking mechanism is positioned in the sliding sleeve and locks the sliding rod when the sliding rod partially extends out of the sliding sleeve; the elastic resetting piece is arranged between the sliding sleeve and the sliding rod; and the magnetic bead adsorption piece is arranged at one end of the sliding rod. The sliding rod capable of extending in the sliding sleeve is adopted, when the push rod pushes the sliding rod to partially extend out of the sliding sleeve, the locking mechanism enables the sliding rod to be locked, the magnetic bead adsorption part is arranged at one end of the sliding rod and can be abutted to a container filled with the to-be-separated object, and therefore the to-be-separated object is magnetically separated from the to-be-separated object, the structure is more compact, and the operation process is simpler and more flexible.

Description

Magnetic separation device and chemiluminescence immunoassay analyzer
Technical Field
The invention belongs to the technical field of chemical analysis devices, and particularly relates to a magnetic separation device and a chemiluminescence immunoassay analyzer.
Background
In the course of chemiluminescence immunoassay, in order to wash away the labeled antibody/antigen which is not combined in the immunoreaction and obtain an effective luminescence signal carried on the magnetic particle compound, the magnetic particle compound and the reaction solution need to be subjected to solid-liquid phase separation by a magnetic separation technology.
In the existing chemiluminescence immunoassay process, a carrier (such as a reaction cup) filled with an object to be separated and a magnetic separator are usually mutually independent, the reaction cup and the magnetic separator are required to be close to each other when magnetic separation is carried out, and the reaction cup and the magnetic separator are separated after the magnetic separation is finished, so that the instrument structure is relatively complex, the volume is large, and the operation is not flexible enough.
Therefore, designing a magnetic separation device and a chemiluminescence immunoassay analyzer is one of the problems of the skilled in the art.
Disclosure of Invention
The embodiment of the invention provides a magnetic separation device, and aims to solve the technical problems that a magnetic separator and a carrier of an object to be separated are mutually independent, the magnetic separation structure is relatively complex, and the operation is not flexible enough in the prior art.
The embodiment of the present invention is achieved as follows, and a magnetic separation apparatus includes: a sliding sleeve; the sliding rod is sleeved in the sliding sleeve; a push rod for pushing the slide rod part to extend out of the sliding sleeve; the locking mechanism is positioned in the sliding sleeve and locks the sliding rod when the sliding rod partially extends out of the sliding sleeve; the elastic resetting piece is arranged between the sliding sleeve and the sliding rod; and the magnetic bead adsorption piece is arranged at one end of the sliding rod.
Further, the locking mechanism includes: a clamping groove arranged on the sliding rod; the clamping piece is arranged on the sliding sleeve and matched with the clamping groove; the clamping piece is clamped into or separated from the rotary driving component of the clamping groove under the pushing of the push rod.
Furthermore, a first inclined plane is arranged on the clamping piece, the rotary driving component comprises a first toothed part arranged on the sliding rod and a second toothed part arranged on the push rod, a second inclined plane matched with the first inclined plane is arranged on the first toothed part, and a third inclined plane matched with the second inclined plane is arranged on the second toothed part.
Further, the magnetic separation device further comprises a boss arranged on the sliding sleeve, a first sliding groove arranged on the sliding rod and matched with the boss, and a second sliding groove arranged on the push rod and matched with the boss, and the clamping piece is arranged at one end of the boss.
Furthermore, one end of the sliding rod is rotatably provided with a placing part, and the magnetic bead adsorption piece is arranged on the placing part.
Furthermore, the magnetic bead adsorption pieces are one piece, and the middle part of the magnetic bead adsorption pieces is provided with an accommodating cavity matched with the outer contour of the container in which the magnetic beads are placed.
Further, the magnetic bead adsorbs the piece and is two, two the corresponding setting of magnetic bead adsorbs the piece in order to form the clamping part of the container that the magnetic bead was placed to the centre gripping.
Further, the magnetic bead adsorption member is a magnet.
Furthermore, the elastic reset piece is a tension spring, one end of the elastic reset piece is connected with the sliding sleeve, and the other end of the elastic reset piece is connected with the placing part.
The invention also provides a chemiluminescence immunoassay analyzer which comprises host equipment, a container for placing reaction liquid containing magnetic beads and the magnetic separation device, wherein the host equipment is provided with a driving mechanism for driving the push rod to move.
The magnetic separation device has the beneficial effects that the sliding rod capable of stretching in the sliding sleeve is adopted, when the push rod pushes the sliding rod to partially stretch out of the sliding sleeve, the locking mechanism enables the sliding rod to be locked, one end of the sliding rod is provided with the magnetic bead adsorption piece, and the magnetic bead adsorption piece is abutted against a container filled with an object to be separated, so that the magnetic separation device is magnetically separated from the object to be separated; the push rod promotes the slide bar once more, and the slide bar drives the magnetic bead and adsorbs the piece and resets, and the magnetic bead adsorbs the piece and breaks away from with the container, and whole magnetic separation device can with hold integrative installation of carrier, and then install on the equipment that is used for chemical analysis, promotes the push rod through external force in the magnetic separation process and can realize adsorbing and break away from two kinds of states, and structural compacter, operation process is also simple nimble more.
Drawings
FIG. 1 is a schematic view of a magnetic separation apparatus according to an embodiment of the present invention;
FIG. 2 is an exploded view of a magnetic separation device according to an embodiment of the present invention;
FIG. 3 is a schematic view of a magnetic separation device according to an embodiment of the present invention with a slide bar locked;
FIG. 4 is an enlarged view taken at A in FIG. 3;
FIG. 5 is a schematic view of a slide bar in an initial state according to an embodiment of the present invention;
FIG. 6 is a schematic view of a slide bar in an ascending state according to an embodiment of the present invention;
fig. 7 is a schematic structural diagram of a magnetic separation device, a container, and a driving mechanism of a chemiluminescent immunoassay analyzer according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Referring to fig. 1 and 2, a preferred embodiment of a magnetic separator according to the present invention comprises:
the sliding sleeve 1 is provided with a cavity inside; the sliding rod 2 is sleeved in the sliding sleeve 1, and the sliding rod 2 is positioned in the cavity in the sliding sleeve 1 and can freely slide in the cavity in the sliding sleeve 1 in the axial direction; the sliding rod 2 is pushed to partially extend out of the push rod 5 of the sliding sleeve 1, but the sliding rod 2 can not be completely separated from the sliding sleeve 1 under the action of the push rod 5; the locking mechanism is positioned in the sliding sleeve 1 and locks the sliding rod 2 when the sliding rod 2 partially extends out of the sliding sleeve 1; the elastic reset piece 4 is arranged between the sliding sleeve 1 and the sliding rod 2; and a magnetic bead adsorption member 3 provided at one end of the slide bar 2.
By adopting the sliding rod 2 which can be stretched in the sliding sleeve 1, when the push rod 5 pushes the sliding rod 2 to partially extend out of the sliding sleeve 1, the locking mechanism locks the sliding rod 2. And slide bar 2 one end is equipped with magnetic bead adsorption part 3, because slide bar 2 is in the locking state, fixes magnetic bead adsorption part 3 and the container that is equipped with the thing that awaits separating together and forms the structure of relatively fixing, and magnetic bead adsorption part 3 can be contradicted with the container that is equipped with the thing that awaits separating to produce magnetic separation with the thing that awaits separating. In addition, an elastic reset piece 4 is arranged between the sliding sleeve 1 and the sliding rod 2, in the extending process of the sliding rod 2, the elastic reset piece 4 gradually stores force, and when the sliding rod 2 is separated from the locking state, the elastic reset piece 4 enables the sliding rod 2 to reset by utilizing the stored force and retracts to the initial position in the sliding sleeve 1.
Referring to fig. 2-6, in particular, the locking mechanism includes:
a clamping groove 21 is arranged on the sliding rod 2, and the clamping groove 21 can be a groove; the clamping piece 12 is arranged on the sliding sleeve 1 and matched with the clamping groove 21; the rotation driving component can make the clamping piece 12 be clamped into or separated from the clamping groove 21 under the pushing of the push rod 5.
The push rod 5 gradually pushes the slide rod 2 to slide in the slide sleeve 1, and the rotary driving component gradually drives the slide rod 2 to axially rotate along with the slide rod 2 gradually extending out of the slide sleeve 1. When the slide bar 2 rotates and rises to a certain degree, the clamping piece 12 is clamped into the clamping groove 21, the push rod 5 does not give a pushing force to the slide bar 2 at the moment, the slide bar 2 has a tendency of resetting movement, but the clamping piece 12 is clamped into the clamping groove 21 to block the slide bar 2 from resetting, so that the slide bar 2 is locked, and the magnetic bead adsorption piece 3 and the object to be separated act. Subsequently, the push rod 5 continues to give a pushing force to the slide rod 2, the rotary driving component drives the slide rod 2 to rotate again, the clamping piece 12 is separated from the clamping groove 21, and the slide rod 2 is reset under the action of the elastic resetting piece 4.
Specifically, referring to fig. 2-6, the card member 12 is provided with a first inclined surface 121, the rotation driving assembly includes a first tooth-shaped portion 22 provided on the sliding rod 2 and a second tooth-shaped portion 51 provided on the push rod 5, the slot 21 may be a groove between adjacent first tooth-shaped portions 22, the first tooth-shaped portion 22 is provided with a second inclined surface 221 matched with the first inclined surface 121, and the second tooth-shaped portion 51 is provided with a third inclined surface 511 matched with the second inclined surface 221. The above-mentioned fingers may be matched in such a way that the inclination angles of the first inclined surface 121 and the second inclined surface 221 are the same and both are effective, and that the inclination angles of the second inclined surface 221 and the third inclined surface 511 are the same and both are effective.
The push rod 5 pushes the slide rod 2 to ascend, when the slide rod 2 extends out of the sliding sleeve 1 to a certain degree, the push rod 5 stops giving thrust to the slide rod 2, the third inclined surface 511 is tangent to the second inclined surface 221, the second inclined surface 221 slides downwards along the third inclined surface 511 along with the resetting trend, and axially rotates and enters the upper part of the first inclined surface 121, at the moment, the clamping piece 12 enters the clamping groove 21, and locking of the slide rod 2 is achieved.
The push rod 5 pushes the slide bar 2 to ascend again, and the second inclined surface 221 follows the ascending of the first inclined surface 121 when the slide bar 2 partially extends out of the sliding sleeve 1 gradually. And when the second inclined surface 221 leaves the first inclined surface 121, that is, the clamping piece 12 is separated from the clamping groove 21, the elastic reset piece 4 generates elastic reset to drive the sliding rod 2 to axially rotate and reset, and then the sliding rod rapidly retracts to the initial position in the sliding sleeve 1.
Further, referring to fig. 2 and 4, the magnetic separation device further includes a boss 11 disposed on the sliding sleeve 1, a first sliding groove 23 disposed on the sliding rod 2 and matched with the boss 11, and a second sliding groove 52 disposed on the push rod 5 and matched with the boss 11, where the shape of the boss 11 is matched with the shape of the cavity on the first sliding groove 23, and the shape of the boss 11 is matched with the shape of the cavity on the second sliding groove 52. The clamping piece 12 is arranged at one end of the boss 11, and the clamping piece 12 and the boss 11 are integrally arranged. The boss 11 is arranged on the inner wall of the sliding sleeve 1 and extends along the axial direction of the sliding sleeve 1, the first sliding chute 23 is arranged on the sliding rod 2 and extends along the axial direction of the sliding rod 2, and the second sliding chute 52 is arranged on the push rod 5 and extends along the axial direction of the push rod 5.
When the push rod 5 does not push the slide rod 2, the slide rod 2 is located at the initial position, and the boss 11 is located in the first slide groove 23 and the second slide groove 52. Along with the ascending of slide bar 2, boss 11 slides on first spout 23 and second spout 52, and first spout 23 and second spout 52 play the guide effect to boss 11. After the second inclined surface 221 enters the first inclined surface 121, the boss 11 is separated from the first sliding groove 23 and the second sliding groove 52, and the card member 12 at one end of the boss 11 smoothly enters the card slot 21. After the second inclined surface 221 leaves the first inclined surface 121, the boss 11 enters the first sliding groove 23 and the second sliding groove 52, and the slide rod 2 is reset.
Optionally, three bosses 11 are uniformly arranged inside the sliding sleeve 1, and the adjacent bosses 11 are spaced by 120 degrees; three first sliding grooves 23 are uniformly arranged on the sliding rod 2, and the interval between every two adjacent first sliding grooves 23 is 120 degrees; six first tooth-shaped portions 22 are arranged on the sliding rod 2, and one first tooth-shaped portion 22 among the adjacent first tooth-shaped portions 22 is connected with the first sliding groove 23; three second sliding grooves 52 are uniformly arranged on the push rod 5, and the interval between every two adjacent second sliding grooves 52 is 120 degrees; the second tooth-shaped portions 51 are six on the push rod 5, and one of the second tooth-shaped portions 51 is arranged to be connected to the second sliding groove 52 in the adjacent second tooth-shaped portions 51. Therefore, the clamping piece 12 can be ensured to be clamped into or separated from the clamping groove 21 in the rotating process of the sliding rod 2, the clamping piece 12 can be clamped into the clamping groove 21 when the sliding rod 2 rotates for the first time, and the clamping piece 12 can be separated from the clamping groove 21 when the sliding rod 2 rotates for the second time.
Of course, the number of the bosses 11, the first slide grooves 23, and the second slide grooves 52 is not limited to the above, but the number of the bosses, the first slide grooves 23, and the second slide grooves 52 is the same. The first tooth portion 22 and the second tooth portion 51 are not limited to the above, but the numbers of the first tooth portion and the second tooth portion are the same. Also, the number of bosses 11 is half of the number of first teeth 22.
Further, referring to fig. 2, one end of the slide bar 2 is rotatably provided with a mounting portion 6, and the magnetic bead adsorption member 3 is disposed on the mounting portion 6. The arrangement part 6 which is rotatably connected with the slide bar 2 is arranged, so that the magnetic bead adsorption piece 3 arranged on the arrangement part 6 keeps still when the slide bar 2 is pushed by the push rod 5 to rotate in the telescopic process. In this embodiment, the magnetic attraction member is a magnet.
Specifically, referring to fig. 2, the slide rod 2 reset member is a tension spring, one end of which is connected to the sliding sleeve 1, and the other end of which is connected to the mounting portion 6. The slide bar 2 resets and is connected with settling part 6, can avoid slide bar 2 rotatory time, and slide bar resets 4 and is twisted round by slide bar 2 drive.
In this embodiment, the magnetic bead adsorbing member 3 may be a single member, and a receiving cavity matching with the outer contour of the container 7 in which the magnetic beads are placed is formed in the middle of the member. Thus, the magnetic bead adsorption member 3 can be ensured to be in contact with the container 7 more comprehensively, and the magnetic beads in the container 7 can be better separated from the reaction solution.
In addition, referring to fig. 2, the magnetic bead adsorbing member 3 may also be two, and the two magnetic bead adsorbing members 3 are correspondingly disposed to form a clamping portion for clamping the container 7 with the magnetic beads. In this embodiment, the mounting portion 6 can be made of two elastic pieces or elastic plastic materials, so that the magnet can be in close contact with the outer wall of the container 7 to better adsorb the magnetic beads in the container 7, and the magnetic beads and the reaction solution can be better separated. Wherein, the two placing parts 6 can be separated or integrally arranged on the slide bar 2 to form a clamping part for clamping the reaction cup, and the magnetic bead adsorption part 3 is arranged at one side of the two placing parts 6 contacted with the container 7.
Referring to fig. 7, the present invention further provides a chemiluminescence immunoassay analyzer, which comprises a host device, a container 7 for containing a magnetic bead reaction solution, and the magnetic separation device, wherein the host device is provided with a driving mechanism 8 for driving the sliding rod 2 to partially extend out of the sliding sleeve 1, and the specific implementation is that the driving mechanism 8 drives the push rod 5 to move, and the push rod 5 pushes the sliding rod 2 to partially extend out of the sliding sleeve 1. The slide bar 2 of the magnetic separation device is driven to stretch by adopting the driving mechanism 8, so that automatic production is realized.
In the present embodiment, the driving mechanism 8 may be one of a motor and a cylinder.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims (10)

1. A magnetic separation device, comprising:
a sliding sleeve;
the sliding rod is sleeved in the sliding sleeve;
a push rod for pushing the slide rod part to extend out of the sliding sleeve;
the locking mechanism is positioned in the sliding sleeve and locks the sliding rod when the sliding rod partially extends out of the sliding sleeve;
the elastic resetting piece is arranged between the sliding sleeve and the sliding rod; and
and the magnetic bead adsorption piece is arranged at one end of the sliding rod.
2. A magnetic separation device according to claim 1 wherein the locking mechanism comprises:
a clamping groove arranged on the sliding rod;
the clamping piece is arranged on the sliding sleeve and matched with the clamping groove;
the clamping piece is clamped into or separated from the rotary driving component of the clamping groove under the pushing of the push rod.
3. The magnetic separator according to claim 2, wherein the latch has a first inclined surface, the rotary driving assembly includes a first tooth portion provided on the sliding rod and a second tooth portion provided on the push rod, the first tooth portion has a second inclined surface matching the first inclined surface, and the second tooth portion has a third inclined surface matching the second inclined surface.
4. The magnetic separation device of claim 3, further comprising a boss disposed on the sliding sleeve, a first sliding groove disposed on the sliding rod and matching with the boss, and a second sliding groove disposed on the push rod and matching with the boss, wherein the clip is disposed at one end of the boss.
5. A magnetic separation device according to any of claims 1 to 4 wherein one end of the slide bar is rotatably provided with a mounting portion, and the magnetic bead adsorption member is disposed in the mounting portion.
6. A magnetic separation device according to claim 5 wherein the magnetic bead adsorbing members are provided in one piece with a receiving chamber in the middle thereof which matches the outer contour of the vessel in which the magnetic beads are placed.
7. The magnetic separation device of claim 5, wherein the magnetic bead adsorption member is provided in two pieces, and the two pieces of the magnetic bead adsorption member are disposed in correspondence to each other to form a holding portion for holding a container in which magnetic beads are placed.
8. The magnetic separation device of claim 5 wherein the magnetic bead adsorbent member is a magnet.
9. A magnetic separator as claimed in claim 5, wherein said resilient return member is a tension spring having one end connected to said sleeve and the other end connected to said mounting portion.
10. A chemiluminescent immunoassay analyzer comprising a host device, a container for containing a reaction solution containing magnetic beads, and the magnetic separation device of any one of claims 1 to 9, wherein the host device is provided with a driving mechanism for driving the push rod to move.
CN201910386204.6A 2019-05-09 2019-05-09 Magnetic separation device and chemiluminescence immunoassay analyzer Pending CN111912975A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910386204.6A CN111912975A (en) 2019-05-09 2019-05-09 Magnetic separation device and chemiluminescence immunoassay analyzer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910386204.6A CN111912975A (en) 2019-05-09 2019-05-09 Magnetic separation device and chemiluminescence immunoassay analyzer

Publications (1)

Publication Number Publication Date
CN111912975A true CN111912975A (en) 2020-11-10

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN203658373U (en) * 2013-12-16 2014-06-18 深圳市亚辉龙生物科技有限公司 Magnetic separation device
CN205301341U (en) * 2015-12-23 2016-06-08 深圳雷杜生命科学股份有限公司 Anticollision formula self - cleaning , mixing and separator
CN208303355U (en) * 2018-02-08 2019-01-01 成都深迈瑞医疗电子技术研究院有限公司 Magnetic bead cleans separator and chemical illumination immunity analysis instrument
CN109107966A (en) * 2018-09-28 2019-01-01 基蛋生物科技股份有限公司 Magnetic bead cleaning device and chemical illumination immunity analysis instrument
US20190022667A1 (en) * 2016-01-19 2019-01-24 Shanxi Zdgsy Bio-Scientific Co., Ltd. Multifunctional biological substance separation device
CN210347657U (en) * 2019-05-09 2020-04-17 深圳华迈兴微医疗科技有限公司 Magnetic separation device and chemiluminescence immunoassay analyzer

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN203658373U (en) * 2013-12-16 2014-06-18 深圳市亚辉龙生物科技有限公司 Magnetic separation device
CN205301341U (en) * 2015-12-23 2016-06-08 深圳雷杜生命科学股份有限公司 Anticollision formula self - cleaning , mixing and separator
US20190022667A1 (en) * 2016-01-19 2019-01-24 Shanxi Zdgsy Bio-Scientific Co., Ltd. Multifunctional biological substance separation device
CN208303355U (en) * 2018-02-08 2019-01-01 成都深迈瑞医疗电子技术研究院有限公司 Magnetic bead cleans separator and chemical illumination immunity analysis instrument
CN109107966A (en) * 2018-09-28 2019-01-01 基蛋生物科技股份有限公司 Magnetic bead cleaning device and chemical illumination immunity analysis instrument
CN210347657U (en) * 2019-05-09 2020-04-17 深圳华迈兴微医疗科技有限公司 Magnetic separation device and chemiluminescence immunoassay analyzer

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