CN212646714U - Chemiluminescence immunoassay reaction disc with multiple reaction modes and analyzer - Google Patents

Abstract

The utility model relates to a technical field is made to full-automatic chemiluminescence immunoassay appearance, specific theory is rational in infrastructure, the reliable operation, can satisfy the chemiluminescence immunoassay reaction dish and the analysis appearance that have many reaction pattern of multi-mode detection demand, be equipped with the ring disk body, set up the circular orbit recess on the disk body, set up the annular on its characterized in that disk body respectively and hatch track and annular function track, set up on the annular function track and be used for realizing adding the sample respectively or adding reagent or mixing or washing or measuring or uninstalled function position, still be equipped with at least one lane change position on the annular function track, set up on the lane change position and be used for realizing current reaction cup transform function track or hatch orbital tongs mechanism, the utility model discloses compare with prior art, have rational in infrastructure, the reliable operation, extensive applicability, advantage that is showing such as detection efficiency height.

Description

Chemiluminescence immunoassay reaction disc with multiple reaction modes and analyzer

The technical field is as follows:

the utility model relates to a technical field is made to full-automatic chemiluminescence immunoassay appearance, specific theory be one kind rational in infrastructure, reliable operation, can satisfy the chemiluminescence immunoassay reaction dish and the analysis appearance that have many reaction pattern of multi-mode detection demand.

Background art:

at present, the requirements of a full-automatic chemiluminescence immunoassay analyzer on the precision and the stability of a detection result are higher and higher, and the detection result has very important functions of guiding medical treatment and operation rescue, so the detection speed directly influences the clinical treatment efficiency. Although the existing equipment realizes the integration of the detection assembly, the detection mode is single, which is mainly because the incubation mechanism, the washing mechanism and the measuring mechanism of the existing equipment are distributed on a single-track turntable, so that each detection sample can be detected only by all the mechanisms. And to different detection demands, there often exist reagent and add the difference that the number of times is different, sample washing number of times is different, and traditional equipment needs to rotate the carousel many times to accomplish to the detection demand that the module setting mode is different on detection mode and the monorail carousel, leads to the problem such as detection time is long, and the testing result is inaccurate.

The invention content is as follows:

the utility model discloses to the shortcoming and not enough that exist among the prior art, provide a rational in infrastructure, reliable operation, can satisfy the chemiluminescent immunoassay dish and the analysis appearance that have many reaction pattern of multi-mode detection demand.

The utility model discloses can reach through following measure:

the utility model provides a chemiluminescence immunoassay reaction dish with many reaction patterns, is equipped with annular disk body, sets up annular track recess on the disk body, sets up annular incubation track and annular function track on its characterized in that disk body respectively, sets up the function position that is used for realizing adding the sample respectively or adding reagent or mixing or washing or measurement or uninstallation on the annular function track, still is equipped with at least one lane change position on the annular function track, sets up the orbital tongs mechanism that is used for realizing present reaction cup transform function track or incubation on the lane change position.

Track and annular function track concentric setting are hatched to the annular, and the preferred annular function track is located the annular and hatches the track outside, is convenient for set up various functional assembly in the disk body outside.

The track lower part is hatched to the annular is equipped with the zone of heating, and the zone of heating is preferred to be constituteed by 7 zones of heating to there is corresponding different heating environment that provides.

Be equipped with on the track is hatched to the annular and hatch the transfer rack, be equipped with function transfer rack on the annular function track, hatch transfer rack and function transfer rack and be the annular, and all set up the reaction cup slotted hole that two above equidistance distribute, wherein reaction cup slotted hole is located track recess top, hatches transfer rack and function transfer rack and is rotated around the carousel centre of a circle by motor drive through gear drive, drives the reaction cup and follows respectively and hatch track and function orbital motion.

Function transfer rack can adopt following structure operation: the side surface of the functional transmission rack is provided with continuous saw teeth meshed with a functional motor gear, and an output shaft of the functional motor drives the functional motor gear to rotate so as to continuously drive the functional transmission rack to operate; furthermore, at least one function transmission auxiliary gear meshed with the outer ring saw teeth of the function transmission rack is arranged on the outer side of the annular disk body, the function transmission auxiliary gear is fixed on the outer side of the turntable through a shaft, and more than two function transmission paying-out gears which are symmetrically distributed are preferably arranged, so that the deviation rectification correction can be performed on the running of the annular function transmission rack.

The incubation transmission rack of the utility model can be driven to rotate by adopting an independent incubation transmission motor, and also can be driven to rotate by a functional motor through a gear transmission mechanism by arranging continuous saw teeth on the outer ring of the incubation transmission rack; further, at least one incubation transmission auxiliary gear is arranged on the inner side of the incubation transmission rack, and the incubation transmission auxiliary gear is meshed with the continuous saw teeth of the inner ring of the incubation transmission rack and is fixed on the annular turntable through a gear shaft and used for correcting the running of the incubation transmission rack.

Set up position sensor on hatching the transfer rack for detect its motion position information, also set up position sensor on the function transfer rack, be used for detecting the motion information of function rack.

The utility model discloses be equipped with two sets of lane change tongs mechanisms on the annular turntable, be used for respectively snatching the reaction cup on the track to the function track, and snatch the reaction cup on the function track to hatching the track.

The utility model discloses it is preferred set gradually on the annular carousel and load district, first adding appearance district, first mixing district, first lane change tongs district, first washing district, second and add appearance district, second mixing district, second lane change tongs district, second washing district, third mixing district, measurement district, uninstallation district.

Annular carousel upper portion lock annular apron.

The utility model also provides a chemiluminescence immunoassay appearance with many reaction pattern, its characterized in that is equipped with above-mentioned annular carousel.

The measuring area of the utility model is provided with a measuring mechanism; the washing district is equipped with the washing mechanism, the mixing district is equipped with the mixing mechanism, the uninstallation district is equipped with consumptive material uninstallation mechanism.

The utility model discloses a set up mutually independent incubation track and function track, make whole equipment can satisfy the demand of many reaction modes, wherein the demand that 7 kinds of reaction modes can be satisfied at least to this scheme, is respectively: loading a reaction cup, adding a sample, adding a reagent 1, uniformly mixing 1, changing a channel 1, incubating, changing a channel 2, washing 2, uniformly mixing 3, measuring and unloading; (2) loading a reaction cup, adding a sample, adding a reagent 1, uniformly mixing 1, changing the channel 1, incubating, changing the channel 1, washing 2, uniformly mixing 3, measuring, unloading (3) loading the reaction cup, adding the sample, adding the reagent 1, uniformly mixing 1, changing the channel 1, incubating, changing the channel 1, washing 2, uniformly mixing 3, measuring and unloading; (4) loading a reaction cup, adding a sample, adding a reagent 1, uniformly mixing 1, changing the channel 1, incubating, changing the channel 1, washing the reagent 2, washing 2, uniformly mixing 3, measuring, unloading (5) loading the reaction cup, adding the reagent 1, uniformly mixing 1, changing the channel 1, incubating, changing the channel 1, washing 1, changing the channel 2, incubating, washing 2, uniformly mixing 3, measuring and unloading; (6) loading a reaction cup, adding a sample, adding a reagent 1, uniformly mixing 1, changing a channel 1, incubating, changing a channel 1, adding a reagent 2, changing a channel 2, incubating, changing a channel 2, washing 2, uniformly mixing 3, measuring and unloading; (7) loading a reaction cup, loading a sample, adding a reagent 1, uniformly mixing 1, changing a channel 1, incubating, changing a channel 1, washing 1, a reagent 2, changing a channel 2, incubating, changing a channel 2, washing 2, uniformly mixing 3, measuring and unloading.

Compared with the prior art, the utility model, have rational in infrastructure, reliable operation, extensive applicability, detection efficiency height and be showing advantage.

Description of the drawings:

fig. 1 is a schematic structural diagram of the ring-shaped turntable of the present invention.

Fig. 2 is a partially enlarged view of fig. 1.

Fig. 3 is a schematic structural diagram of an embodiment of the present invention.

Fig. 4 is a schematic view of a transfer rack according to the present invention.

Fig. 5 is a schematic view of a driving mode of the transfer rack for incubation according to the present invention.

Fig. 6 is a schematic view of a correction mode of the middle incubation transfer rack of the present invention.

Fig. 7 is a schematic view of a heating structure of the middle incubation track of the present invention, wherein fig. 7(a) is a schematic view of a division of a heating zone, and fig. 7(b) is a schematic view of a heating layer structure.

Fig. 8 is a schematic structural diagram of a functional position for loading a medium function transmission rack according to the present invention.

Fig. 9(a) is a cross-sectional view of the measurement site, and fig. 9(b) is a plan view of the measurement site.

Fig. 10 is a schematic structural view of a baffle bottom plate.

Fig. 11 is a schematic view of the structure of the measuring mechanism.

Fig. 12 is a schematic diagram of the structure of the unloading mechanism, wherein fig. 12(a) is a schematic diagram of the structure of the unloading mechanism, fig. 12(b) is a schematic diagram of the unloading paddle in an unloading initial state, and fig. 12(c) is a schematic diagram of the structure of the unloading paddle in unloading.

Fig. 13 is a schematic diagram showing a structure of a washing mechanism in example 2, fig. 13(a) is a schematic diagram showing a structure of a washing mechanism in example 2, fig. 13(b) is a schematic diagram showing a structure of a liquid injection and suction combination needle, and fig. 13(c) is a schematic diagram showing a structure of a magnet mounting plate.

FIG. 14 is a schematic view showing the structure of a washing mechanism in example 5, FIG. 14(a) is a schematic view showing the whole of the washing mechanism, FIG. 14(b) is a schematic view showing the distribution of magnets in each functional position of a washing zone in example 5, and FIG. 14(c) is a plan view showing each functional position of the washing zone in example 5.

FIG. 15 is a schematic structural view of a kneading mechanism in embodiment 3, FIG. 15(a) is a schematic structural view of the kneading mechanism, and FIG. 15(b) is a schematic structural view of an electric motor and a rotating shaft in the kneading mechanism; FIG. 15(c) is a schematic view of another embodiment of the kneading mechanism; FIG. 15(d) is a schematic structural view of a kneading sleeve in the kneading mechanism, and FIG. 15(e) is a sectional view of FIG. 15(d) taken along the A-A direction.

Reference numerals: the plate body 1, the incubation track 2, the functional track 3, the external temperature zone 4, the internal temperature zone 5, the water outlet 6, the incubation transmission rack 7, the functional transmission rack 8, the reaction cup slot hole 9, the functional motor gear 10, the functional motor 11, the functional transmission auxiliary gear 12, the position sensor 13, the incubation motor 14, the baffle lifting slot 15, the cup position 16 to be detected, the detection cup position 17, the detected cup position 18, the photon counter 19, the waste liquid position 20, the incubation transmission auxiliary gear 21, the lane change gripper mechanism 22, the loading zone 23, the first sample adding zone 24, the first lane change gripper zone 25, the first washing zone 26, the second sample adding zone 27, the second blending zone 28, the second lane change gripper zone 29, the second washing zone 30, the third blending zone 31, the measuring zone 32, the unloading zone 33, the first blending zone 34, the loading position 35, the loading position sensor 36, the light blocking bottom plate 37, the waste liquid cup position 38, the functional motor gear 10, the functional motor gear 11, the functional, An annular cover plate 39, a linear lifting motor 40, a linear lifting motor fixed bottom plate 41, a fixed side plate 42, a light barrier position blocking plate 43, a position sensor 44, a left end blocking plate 45, a right end blocking plate 46, a left detection cup position blocking plate 47, a right detection cup position blocking plate 48, an annular boss 49, a liquid storage tank 50, a photon counter 51, a photon counter fixed plate 52, a magnet mounting plate 53, a magnet mounting groove 54, a magnet 55, a pre-adsorption magnet mounting groove 56, a lifting needle frame 57, a liquid absorption and injection combined needle 58, a liquid absorption needle 59, a liquid injection needle 60, a reaction cup 61, a bottom plate 62, a vertical plate 63, a pressing plate 64, a split tooth 65, a pre-adsorption region 66, an adsorption region 67, a non-filling region 68, a blending sleeve 69, a rotating shaft 70, a plug pin 71, a spiral groove hole 72, an eccentric hole 73, a motor 74, an inner layer sleeve 75, an outer layer sleeve, Diversion trench 80, position sensor 81, support ring 82, water guide hole 83 and magnet 84.

The specific implementation mode is as follows:

the present invention will be further described with reference to the accompanying drawings and examples.

As shown in the attached drawings 1 and 2, the utility model provides a chemiluminescence immunoassay reaction disc with multiple reaction modes, which is provided with an annular disc body 1, an annular track groove is arranged on the disc body 1, an annular incubation track 2 and an annular function track 3 are respectively arranged on the disc body, a function position which is respectively used for adding samples or reagents or uniformly mixing or washing or measuring or unloading is arranged on the annular function track 3, at least one lane changing position is also arranged on the annular function track 3, and a gripper mechanism which is used for realizing the function changing track 3 of the current reaction cup or the incubation track 2 is arranged on the lane changing position; the annular incubation track 2 and the annular functional track 3 are arranged concentrically, and preferably, the annular functional track 3 is positioned on the outer side of the annular incubation track 2, so that various functional components can be conveniently arranged on the outer side of the disc body 1; as shown in fig. 7, a bottom heating layer 7(b) is arranged at the lower part of the annular incubation track 2, the heating layer preferably consists of 7 heating zones which can be divided into an outer temperature zone 4 and an inner temperature zone 5 respectively, and a water outlet 6 is arranged; thereby providing different heating environments in a targeted manner.

As shown in fig. 4, 5, 6, be equipped with on the track 2 is hatched to the annular and hatch transfer rack 7, be equipped with function transfer rack 8 on the annular function track 3, hatch transfer rack 7 and function transfer rack 8 are the annular, and all set up the reaction cup slotted hole 9 that two above equidistance distribute, wherein reaction cup slotted hole 9 is located track recess top, hatches transfer rack 7 and function transfer rack 8 and is rotated around the 1 centre of a circle of disk body by motor drive through gear drive, drives the reaction cup and moves along hatching track 2 and function track 3 respectively.

As shown in fig. 8, the function transfer rack 8 of the present invention can operate with the following structure: the side surface of the functional transmission rack 8 is provided with continuous saw teeth meshed with the functional motor gear 10, and an output shaft of the functional motor 11 drives the functional motor gear 10 to rotate so as to continuously drive the functional transmission rack 8 to operate; furthermore, at least one function transmission auxiliary gear 12 meshed with the outer ring saw teeth of the function transmission rack 8 is arranged on the outer side of the annular disc body 1, the function transmission auxiliary gear 12 is fixed on the outer side of the disc body 1 through a shaft, preferably more than two function transmission paying-out gears 12 which are symmetrically distributed are arranged, and deviation rectification correction can be carried out on the operation of the annular function transmission rack 8.

As shown in fig. 5, the incubation transmission rack 7 of the present invention can be driven by a separate incubation transmission motor 14, or can be driven by a functional motor to rotate through a gear transmission mechanism by arranging continuous saw teeth on the outer ring of the incubation transmission rack 7; further, at least one incubation conveying auxiliary gear 21 is provided inside the incubation conveying rack 7, and the incubation conveying auxiliary gear 21 is engaged with the inner ring continuous saw teeth of the incubation conveying rack 7 and fixed on the annular turntable through a gear shaft for correcting the operation of the incubation conveying rack 7.

Incubate and set up position sensor 13 on the transfer rack 7 for detect its motion position information, also set up position sensor 13 on the function transfer rack 8, be used for detecting the motion information of function rack.

The utility model discloses be equipped with two sets of lane-changing tongs mechanisms 22 on the ring disk body 1, be used for respectively snatching the reaction cup on the track 2 of will hatching and snatch to function track 3 and snatch the reaction cup on the function track 3 to hatching track 2.

The utility model discloses it is preferred to be in set gradually loading district 23, first sample application district 24, first mixing district 34, first lane changing tongs district 25, first washing district 26, second sample application district 27, second mixing district 28, second lane changing tongs district 29, second washing district 30, third mixing district 31, measurement area 32, uninstallation district 33 on the ring-shaped plate body 1, wherein as shown in figure 8, for loading position 35, load and set up frame loading auxiliary stand and load position sensor 36 on the position 35 at the reaction cup slotted hole that loading district 23 corresponds.

Annular disk body 1 goes up lock annular apron 39.

The utility model also provides a chemiluminescence immunoassay appearance with many reaction pattern, its characterized in that is equipped with above-mentioned annular disk body 1.

The measuring area of the utility model is provided with a measuring mechanism; the washing district is equipped with the washing mechanism, the mixing district is equipped with the mixing mechanism, the uninstallation district is equipped with consumptive material uninstallation mechanism.

Example 1:

as shown in fig. 9 to 11, the utility model sets a detection position at the functional track 3 corresponding to the measurement area, the detection position is composed of a cup position 16 to be detected, a detection cup position 17 and a detected cup position 18 which are sequentially arranged along the conveying direction of the reaction cup, baffle lifting slots 15 penetrating through the annular turntable body 1 are respectively arranged on the functional track 3 corresponding to the cup position 16 to be detected, the detection cup position 17 and the detected cup position 18, and the cup position 16 to be detected, the detection cup position 17 and the detected cup position 18 are respectively arranged between two adjacent baffle lifting slots 15; a photon counter fixing hole communicated with the functional track 3 is formed in the annular disc body 1 corresponding to the detection cup position 17, and the photon counter 19 extends into the fixing hole to count reaction cups passing through the detection cup position 17; as shown in fig. 10, a baffle lifting mechanism is arranged below the annular disc body 1, the baffle lifting mechanism is provided with a light blocking bottom plate 37, the upper surface of the light blocking bottom plate 37 is respectively provided with a detection baffle for separating the detection position and the to-be-detected cup position 16, the detection cup position 17 and the detected cup position 18, and a linear lifting motor 40 for driving the light blocking bottom plate to lift is further arranged; the detection baffles in the baffle lifting mechanism are respectively inserted into two ends of the detection position along the baffle lifting slot 15 and between the cup position 16 to be detected, the cup position 17 to be detected and the detected cup position 18, the light blocking bottom plate 37 seals the bottom of the annular disk body 1, the annular cover plate 39 seals the upper part of the annular disk body 1, and the groove type functional track 3 corresponding to the detection position of the annular disk body 1 forms a darkroom.

In this embodiment, the reaction cup 61 has an annular boss at its rim for supporting the reaction cup on the reaction cup slot of the transmission rack, and moves forward along the functional track, sequentially passes through the cup position 16 to be detected, the cup position 17 to be detected, the cup position 18 to be detected, and the waste liquid cup position 38, the baffle plate lifting mechanism is provided with a linear lifting motor 40 fixed at the lower side of a linear lifting motor fixing bottom plate 41, the linear lifting motor fixing bottom plate 41 is provided with a shaft hole, an output shaft of the linear lifting motor 40 extends out through the shaft hole and is connected with a light blocking bottom plate 37, a fixing side plate 42 connected with the annular plate body is arranged at the upper side of the linear lifting motor fixing bottom plate 41, a light blocking plate position blocking plate 43 for limiting the downward moving position of the light blocking bottom plate 37 and the detection baffle plate is arranged on the fixing side plate 42, and a position sensor 44 for detecting the light blocking bottom plate and the baffle plate position is further arranged, can be connected and fixed through a position sensor fixing plate connected with the fixed side plate;

in order to reduce the impact of the light blocking bottom plate 37 when the detection baffle is inserted, a spring is arranged between the top disc of the linear lifting motor 40 and the light blocking bottom plate, wherein a shaft sleeve is arranged in the center of the bottom of the light blocking bottom plate 37, a spring is arranged in the shaft sleeve, an output shaft of the linear lifting motor 40 extends into the shaft sleeve, and the top disc at the front end of the output shaft is contacted with the spring;

as shown in fig. 10, the detection baffles on the upper surface of the light-blocking bottom plate in the baffle elevating mechanism include a left end baffle 45 and a right end baffle 46 which are positioned at two ends and used for sealing two ends of the detection position, and further include a left detection cup position baffle 47 and a right detection cup position baffle 48 which are positioned between the left end baffle 45 and the right end baffle 46 and used for sealing two ends of the detection cup position, wherein the four detection baffles have the same height, and when the baffle elevating mechanism ascends to the highest point and the detection baffles are respectively inserted into two ends of the detection position and between the to-be-detected cup position, the detection cup position and the detected cup position along the baffle elevating slot, the detection baffles are flush with the surface of the annular disk body, so as to form a flat working surface which is beneficial; in order to further improve the light blocking effect on the detection cup position, the cross sections of the left detection cup position baffle 47 and the right detection cup position baffle 48 are symmetrically arranged in an L shape, and the bent part of the left detection cup position baffle/the right detection cup position baffle is close to the photon counter end and is folded outwards to the position far away from the reaction cup, so that the detection cup position forms an independent small darkroom inside under the sealing of the upper cover, the light blocking bottom plate and the detection baffle, and photons cannot enter the darkroom;

the upper surface of the light blocking bottom plate in the baffle lifting mechanism is provided with the double annular bosses 49 around the four detection baffles to form a reversed-square cofferdam, and the double annular grooves matched with the double annular bosses are arranged at the corresponding positions on the bottom surface of the annular turntable disc body to improve the fitting degree of the light blocking bottom plate and the bottom surface of the annular disc body, further improve the light blocking effect and prevent excitation liquid from mistakenly hitting and overflowing; further, a liquid storage groove 50 is arranged at a position between the left detection cup position baffle and the right detection cup position baffle on the upper surface of the light blocking bottom plate and used for temporarily storing the mistakenly-beaten excitation liquid or the low-falling waste liquid to avoid polluting other parts of the equipment.

Set up camera lens glass mount in the photon counter fixed orifices of seting up on the annular turntable lateral wall to set up photon counter camera lens glass, photon counter 51 stretches into in the fixed orifices, and fixes through the photon counter fixed plate, sets up O type circle between photon counter 51 and the fixed orifices, and photon counter fixed plate 52 is connected fixedly with fixed curb plate.

The utility model discloses a set up by linear elevator motor driven baffle elevating system, according to the data acquisition of position sensor position, control the bottom plate that is in the light and the detection baffle up-and-down motion on the bottom plate that is in the light, after reaction cup transport mechanism will await measuring the reaction cup and send into the detection cup position, bottom plate and detection baffle in the light rise, bottom plate and detection baffle in the light form a darkroom environment with annular disk body and upper cover, and form independent closed darkroom respectively to the three reaction cups in detecting the position, utilize photon counter to carry out the reading to the reaction cup that detects the cup position, two reaction cups adjacent with this reaction cup are all isolated outside independent darkroom this moment, therefore the luminescent material in the adjacent reaction cup can not produce the interference to the testing result; in addition, in order to realize good sealing, the two components are required to be absolutely and tightly matched, and in order to reduce the impact on the linear motor, the top disc of the linear lifting motor is matched with the spring to be contacted with the light blocking bottom plate, so that the flexible sealing of the light blocking bottom plate is realized; the upper limit and the lower limit of the light barrier lifting mechanism are realized by arranging the light barrier position blocking piece; by arranging the lens glass, the photon counter is effectively protected.

Example 2:

washing mechanism adopts following structure:

as shown in fig. 13, the washing area is provided with a magnetic bead adsorption mechanism, a magnet mounting plate 53 is arranged in the magnetic bead adsorption mechanism, the magnet mounting plate 53 is mounted on the side surface of the functional track 3, more than two magnet mounting grooves 54 are formed in the magnet mounting plate 53, two magnets 55 are arranged in each magnet mounting groove 54 in parallel up and down, and the magnets 55 face the reaction cups passing through the functional track 3; the magnetic pole directions of the two magnets 55 positioned in the same magnet mounting groove 54 are reversed, and the magnetic attraction directions of the two magnets positioned at the same height in the adjacent magnet mounting grooves 54 are also reversed; further correspond on the magnet mounting panel or the function track lateral wall that the entrance in washing district corresponds and adsorb processing initial position department and still be equipped with in advance and adsorb magnet mounting groove 56, magnet of installation in advance in the magnet mounting groove 56.

The utility model discloses correspond adsorption treatment initial position department on magnet mounting panel 53, rotate the direction of advance along ring disk body 1, the parallel two magnet mounting grooves 56 that adsorb in advance that set up respectively in every magnet mounting groove that adsorb in advance for before the washing process begins, carry out adsorption treatment in advance to the magnetic bead in the reaction cup, improve and adsorb the cleaning performance.

A lifting needle frame 57 is further arranged on the annular cover plate 39 on the upper side of the tray body 1 in the washing area, the magnetic bead adsorption mechanism is arranged on the side surface of the functional track 3, and the lifting support 57 is arranged above the functional track; it annotates liquid imbibition combination needle 58 to be equipped with at least two sets of notes on the lift needle frame 57, it comprises a imbibition needle 59 and an notes liquid needle 60 to annotate liquid imbibition combination needle 58, wherein imbibition needle 59 lower extreme needle point position is less than notes liquid needle 60 lower extreme needle point position, and annotates the needle point of liquid needle 60 to the direction of being close to imbibition needle 59 is buckled, thereby when guaranteeing to absorb the waste liquid, the needle point of imbibition needle can reach the interior sample bottom of reaction cup, fully siphons away impurity, and when pouring into the washing liquid, the needle point of annotating the liquid needle is located imbibition needle point top, the washing liquid that the notes liquid needle injected can wash the imbibition needle, in order to avoid polluting different samples.

Further, the liquid suction needle 59 and the liquid injection needle 60 are arranged on one side of the reaction cup deviating from the magnet adsorption mechanism through the lifting needle frame 57, so that the liquid suction needle is ensured to descend to the opposite side of the reaction cup where magnetic beads are adsorbed and agglomerated when sucking liquid, and the magnetic beads are prevented from losing.

The magnet mounting plate 53 comprises a vertical plate and a bottom plate 62, the vertical plate 63 and the bottom plate 62 are vertically and fixedly connected, the cross section of the vertical plate is L-shaped, more than two U-shaped grooves for placing magnets are formed in the side face of the vertical plate 63, the opening direction of each U-shaped groove is upward, a pressing plate 64 for closing the openings of the more than two U-shaped grooves is arranged at the top of the vertical plate, the pressing plate is fixedly connected with the top of the vertical plate through screws, and the bottom plate is provided with splicing teeth for being embedded with a; two magnets which are arranged side by side up and down are arranged in a U-shaped groove on the side face of the vertical plate, and an antirust iron plate is arranged on the outer side of each magnet and used for strengthening magnetism; the vertical plate and the bottom plate are both in an arc shape which is matched with the inner side of the functional track on the tray body, the corresponding position of the washing area on the inner wall of the functional track of the tray body is provided with an installation gap which is used for assembling the magnet installation plate 53 and is communicated with the functional track, and the installation gap is provided with an installation groove which is matched with the assembling teeth 65.

In the using process, the reaction cup tray body 1 drives more than two reaction cups for containing samples to be detected to move forward according to the set direction, the driving mechanism for driving the reaction cup rotating disc to rotate can drive the reaction cup rotating disc to advance one reaction cup position at a time, when the washing treatment is started, the reaction cup firstly enters the pre-adsorption treatment area under the drive of the rotating disk, the single magnet in the pre-adsorption magnet installation groove on the magnet installation plate carries out pre-adsorption treatment on the magnetic beads in the reaction cup, thereby leading the magnetic beads originally scattered in the whole space of the reaction cup to be close to the side where the magnetic bead adsorption mechanism is positioned, when the rotating disc drives the reaction cup to enter the double-particle magnet adsorption area, the magnetic beads are quickly adsorbed on the cup wall of the reaction cup close to one side of the magnet, and at the moment, due to the adoption of double-particle magnet adsorption, the magnetic beads in the reaction cup are flatly paved on the cup wall of the reaction cup, so that the agglomeration phenomenon is effectively reduced, and the influence of impurities wrapped by multi-layer agglomerated magnetic beads on the cleaning effect is avoided; meanwhile, the lifting needle frame is driven by the needle frame driving mechanism to lift to complete the injection of the cleaning liquid and the absorption of the waste liquid, wherein the needle point position of the lower end of the liquid suction needle is lower than the needle point position of the lower end of the liquid injection needle, and the needle point of the liquid injection needle is bent towards the direction close to the liquid suction needle, so that when the waste liquid is absorbed, the needle point of the liquid suction needle can reach the bottom of the sample in the reaction cup to fully absorb impurities, when the cleaning liquid is injected, the needle point of the liquid injection needle is positioned above the needle point of the liquid suction needle, and the cleaning liquid injected by the liquid injection needle can flush the liquid suction needle, so that different; in addition, the liquid suction needle and the liquid injection needle are arranged on one side of the reaction cup above the magnet adsorption mechanism through the lifting support, so that the liquid suction needle is ensured to descend to the opposite side of the reaction cup where magnetic beads are adsorbed and agglomerated when sucking liquid, and the magnetic beads are prevented from losing.

Compared with the prior art, the utility model, under the prerequisite that does not increase the washing number of times, can effectively improve the cleaning performance to reduce the loss of magnetic bead, have rational in infrastructure, easy and simple to handle, advantages that reliable operation etc. are showing.

Example 3:

mixing mechanism adopts following structure:

as shown in fig. 15, a blending mechanism is fixed on the functional track 3 corresponding to the blending region, so that a blending sleeve in the blending mechanism is positioned below a reaction cup in the functional track 3 of the blending region; the blending sleeve 69 is sleeved outside the rotating shaft 70, a bolt 71 is arranged on the rotating shaft 70, a spiral groove hole 72 arranged around the barrel is formed in the barrel of the blending sleeve 69, and two ends of the bolt 71 are located in the spiral groove hole 72; the upper end opening of the blending sleeve 69 is used for accommodating the reaction cup 61, and the inner diameter circle and the outer diameter circle of the upper end opening of the blending sleeve are eccentrically arranged so as to form an eccentric hole 73; the shaft is connected to the motor output shaft and the motor 74 drives the shaft to rotate the latch.

In order to obtain adjustable rotational inertia, the blending sleeve 69 adopts a double-layer sleeve structure, the double-layer sleeve is made of different materials, the inner-layer sleeve 75 is made of PPS plastic, and the outer-layer sleeve 76 is made of a metal sleeve; the double-layer sleeve structure can ensure that the rotational inertia of the whole mixing sleeve is adjustable, and the excessive or small rotational inertia is avoided.

The spiral slot 72 of the utility model is located in the middle lower part of the mixing sleeve 69, so that when the rotating shaft rotates, the bolt drives the mixing sleeve to move, and drives the mixing sleeve to ascend along the rotating shaft through the rotation inertia, at the moment, the bolt on the rotating shaft gradually rotates to the low position along the high position of the spiral slot, the upper port of the mixing sleeve ascends and holds the lower end of the current reaction cup in the upper port, the motor 74 continues to rotate positively, the rotating shaft continues to rotate, the mixing sleeve is driven to rotate and vibrate the reaction cup located at the upper port of the mixing sleeve 69, and the vibration mixing is completed; when the motor 74 rotates reversely, the rotating shaft rotates reversely, and the bolt rotates to a high position along the low position of the spiral groove hole to drive the blending sleeve to fall.

The utility model is provided with a bracket, the bracket comprises a base 77 and a mixing sleeve bracket 78, the mixing sleeve bracket 78 is connected with the base through a vertical plate 79, the base 77 is provided with a shaft hole, a motor 74 is positioned below the base, and a motor output shaft extends out through the shaft hole to be connected with a rotating shaft above the base; the mixing sleeve support 78 is positioned above the base and is provided with a through hole for accommodating the mixing sleeve, and a gap is formed between the wall of the through hole and the outer wall of the mixing sleeve; because the sample to be processed is liquid, so there is the risk of spilling, consequently further set up guiding gutter 80 on the upper surface of mixing sleeve support for to the liquid of spilling water conservancy diversion, avoid liquid to get into inside the equipment.

The utility model is also provided with an in-place detection circuit which is used for detecting whether the mixing sleeve 2 falls back to the base or not, because the device is used in the continuous detection equipment, after the mixing is finished, the mixing sleeve falls back to the bottom of the rotating shaft on the base, the reaction cup is separated from the upper port of the mixing sleeve, then enters the next stage of working procedure through a conveying mechanism, and the problem of cup clamping can be caused if the mixing sleeve can not fall back in time, the whole detection working procedure can be interrupted, the sample to be detected and the equipment can be damaged, in order to avoid the problem, the position sensor 81 fixed on the base is arranged in the in-place detection circuit of the utility model, the position sensor detects whether the blending sleeve falls back to the lowest position or not, if the blending sleeve falls back, the motor is not started to rotate reversely, otherwise, the motor is controlled to rotate reversely through the motor control circuit, and therefore the rotating shaft drives the bolt to lower the uniformly mixing sleeve onto the base.

The utility model discloses the mixing sleeve outside is equipped with support ring 82, support ring 82 can adopt boss or the annular plate that sets up around mixing sleeve outer wall to realize, and the support ring is located mixing sleeve upper portion and the external diameter is greater than the external diameter of mixing sleeve support upper through-hole, is used for supporting mixing sleeve on mixing sleeve support's through-hole under quiescent condition, and can rotate the start-up in-process, and the through-hole upper wall on mixing sleeve support takes place the interaction force, impels mixing sleeve to take place relative rotation, and then rotatory rising; the annular plate is preferably adopted, the annular plate not only can play a supporting effect, but also can prevent a liquid sample in the mixing sleeve, which is used for placing the reaction cup, from pouring into the sleeve, and corrosion of equipment is avoided.

The epaxial bolt adopts the POM plastics that have self-lubricating characteristic to make, is the bar, sets up the horizontal bolt hole that link up the pivot in the pivot, inserts the bolt downthehole with the bolt, because the device can rotate along with the motor is high-speed at the operation in-process, in order to avoid the bolt to be thrown away, further sets up the fixed orifices along pivot length direction setting in the pivot, the fixed orifices link up with horizontal bolt slotted hole, will compress tightly a fixed orifices of impressing and offset with the bolt in the fixed orifices, fixed orifices upper end reuse screw locking.

The utility model discloses further set up the water guide hole 83 that is used for discharging the discharge overflow at mixing sleeve upper portion lateral wall.

The utility model discloses in order to avoid the reaction cup in the mixing sleeve to be thrown away, can set up the roof on mixing sleeve upper portion.

The utility model discloses carry out the mixing to the chemiluminescence immunoassay sample, because the active ingredient in this type of sample is carried by the magnetic bead, in order to avoid the magnetic bead to reunite in a large number, can set up two magnets relatively at mixing sleeve support's upper surface, wherein two magnet 84 are close to the magnetic pole of mixing sleeve one end opposite, thereby make the reaction cup at the in-process of mixing, pass the magnetic field repeatedly, the magnetic bead in the reaction cup is under the effect of magnetic field magnetic force, form relative motion with the reaction cup, thereby improve the mixing effect.

Compared with the prior art, the utility model provides the shaking sleeve with adjustable rotational inertia, which can flexibly select the rotating speed of the shaking sleeve after being lifted, and can drive the shaking sleeve to be quickly lifted under the condition of low acceleration; the blending effect is flexibly adjusted under the condition of not changing the motor drive; and can be compatible with a plurality of motors, has good mixing effect and basically eliminates bubbles.

Example 4:

consumptive material uninstallation mechanism adopts following structure:

as shown in attached figure 12, the cup body of the reaction cup in the utility model is in a flat-mouth strip shape, furthermore, the cross section of the cup body of the reaction cup is in a rectangle shape, in the conveying process, the main body part of the reaction cup body is accommodated on the functional track, the cup wall with larger area of the reaction cup body faces to one side provided with the photon counter, wherein the transverse width of the reaction cup slotted hole 9 on the function transmission rack 8 is larger than the length of the cup mouth of the reaction cup, the width in the moving direction is less than the length of the mouth of the reaction cup, the arrangement mode is used for facilitating the unloading of the waste reaction cup, an unloading mechanism is arranged on the unloading area of the functional track 3, the reaction cup unloading mechanism comprises an unloading motor 53 and an unloading shifting sheet 54, wherein, a motor shaft hole is arranged on the cover body above the unloading area disc body, and an unloading motor 53 is fixed above the top cover of the unloading area and is connected with an unloading shifting sheet 54 positioned below the unloading top cover along the motor shaft hole; the unloading shifting piece 54 is fixedly connected with the motor through a cotter pin, the unloading shifting piece 54 can be provided with a first shifting boss 55 and a second shifting boss 56 which are respectively positioned at two sides of the reaction cup, when unloading, the motor drives the unloading shifting piece to rotate 90 degrees, and the first shifting boss 55 and the second shifting boss 56 simultaneously shift the upper port of the reaction cup 57 to rotate; further, the unloading motor is fixed on the unloading top cover through the motor base, further, an unloading sensor can be arranged on the unloading top cover, whether the reaction cup to be unloaded exists on the current unloading position or not is detected through the unloading sensor, if the reaction cup to be unloaded exists on the current unloading position, the unloading motor is controlled to execute rotation action through the motor control circuit, otherwise, unloading is not carried out, and the overall processing efficiency is improved.

A discharging channel 58 for guiding the reaction cup falling from the reaction cup slotted hole 9 into the waste bucket is arranged below the corresponding discharging position on the functional track 3, and the waste bucket is arranged below the discharging channel.

Example 5:

washing mechanism adopts two side washing modes, adopts following structure:

as shown in fig. 14, the two-sided washing mechanism is provided with a first magnetic bead adsorption mechanism and a second magnetic bead adsorption mechanism separately arranged at two sides of the functional track 3 of the washing area, wherein both the first magnetic bead adsorption mechanism and the second magnetic bead adsorption mechanism are provided with magnets, and the magnets in the first magnetic bead adsorption mechanism and the second magnetic bead adsorption mechanism are asymmetrically arranged relative to the functional track.

All be equipped with the magnet mounting panel 53 that is used for fixed magnet in first magnetic bead adsorption apparatus structure/second magnetic bead adsorption apparatus structure, magnet mounting panel 53 sets up the magnet mounting groove towards one side of function track 3, preferably set up magnet mounting groove 54 more than two, be used for installing two sets of magnet more than respectively, thereby accomplish many times magnetism and inhale at the orbital in-process of sample through washing district function, magnet mounting groove on the magnet mounting panel is seted up for function track asymmetry in first magnetic bead adsorption apparatus structure and the second magnetic bead adsorption apparatus structure, thereby make the sample by the crisscross absorption of both sides magnetic force adsorption apparatus structure, guarantee that the last absorption back magnetic bead fully scatters, carry out next absorption again, avoid magnetic bead layering parcel impurity.

A plurality of sample reaction cups 61 containing samples to be processed continuously pass through the functional track 3, a liquid sucking/injecting needle assembly is further arranged above the functional track 3, more than two reaction cups 61 in the figure continuously run from right to left, a pre-adsorption area 3 passes through firstly, a single magnet can be arranged at a pre-adsorption position in the pre-adsorption area 3, two groups of pre-adsorption positions are continuously arranged in the pre-adsorption area 3, and each group corresponds to the unit displacement distance (advancing step length of the reaction cups driven by a transmission rack) of the reaction cups 2 one by one;

an adsorption area 67 is arranged behind the pre-adsorption area 66, the magnet mounting plate 53 in the adsorption area 67 is filled with adsorption positions of double magnets, the adsorption positions are also continuously arranged in more than two, a non-filling area 68 is arranged on the magnet mounting plate 53 on the same side with the adsorption positions, and a filling area is arranged on the magnet mounting plate on the other side (the magnet mounting plate 53 on the other side corresponding to the adsorption areas and the pre-adsorption areas is a non-filling area), at the moment, the full-filling mode in FIG. 14 can be adopted, but a non-full-filling mode can be further selected, namely, the magnet mounting positions, close to the entering direction of the reaction cup, in the more than two magnet mounting positions on the magnet mounting plate 53 on the other side can be in a non-filling state, and the structure can further improve the dispersion effect of the wrapped impurities;

the magnet mounting plate on the side where the pre-adsorption region 66 is provided in the drawing is provided with an adsorption region 67 again on the other side of the non-filling region 68, and more than two sets of double-magnet filling positions are provided in the adsorption region 67.

Through the multiple alternate adsorption, the impurities wrapped in the sample are fully removed; in addition, in the alternative adsorption process, more than two continuous magnet installation positions (no matter filling areas or non-filling areas) are guaranteed by the turning of each magnetic adsorption, and by means of the structure, the magnetic force conflict of adjacent magnets can be effectively avoided, and the adsorption reliability of magnetic beads of adjacent areas is guaranteed.

The magnet mounting plate structure is similar to that of the embodiment 2, more than two magnet mounting grooves are formed in the magnet mounting plate structure, two magnets are arranged in each magnet mounting groove 11 side by side up and down, the magnetic pole directions of the two magnets positioned in the same magnet mounting groove are reversed, and the magnetic attraction directions of the two magnets positioned at the same height in the adjacent magnet mounting grooves are also reversed; because double-particle magnet adsorption is adopted, the magnetic beads in the reaction cup are flatly paved on the cup wall of the reaction cup, the agglomeration phenomenon is effectively reduced, and the influence of impurities wrapped by multi-layer agglomerated magnetic beads on the cleaning effect is avoided;

the washing mechanism in the embodiment is particularly suitable for a reaction rotary disk type chemiluminescence immunoassay analyzer, wherein a functional track through which a sample passes is arranged on the reaction rotary disk; two pre-adsorption magnet mounting grooves are arranged in parallel at the position, corresponding to the adsorption treatment starting position, on the magnet mounting plate along the rotating advancing direction of the rotating disc of the reaction cup, and a magnet is arranged in each pre-adsorption magnet mounting groove and used for performing pre-adsorption treatment on magnetic beads in the reaction cup before the washing process starts, so that the adsorption cleaning effect is improved; compared with the prior art, can carry out both sides to the pending sample of process in the function track and adsorb many times to when effectively solving current unilateral and adsorbing, the impurity of parcel can not fully scatter the problem that the impurity that causes can't clear away between the magnetic bead, have rational in infrastructure, easy and simple to handle etc. and show advantage.

Claims (10)

1. The utility model provides a chemiluminescence immunoassay reaction dish with many reaction patterns, is equipped with annular disk body, sets up annular track recess on the disk body, sets up annular incubation track and annular function track on its characterized in that disk body respectively, sets up the function position that is used for realizing adding the sample respectively or adding reagent or mixing or washing or measurement or uninstallation on the annular function track, still is equipped with at least one lane change position on the annular function track, sets up the orbital tongs mechanism that is used for realizing present reaction cup transform function track or incubation on the lane change position.

2. The chemiluminescent immunoassay reaction plate with multiple reaction modes according to claim 1, wherein the annular incubation track and the annular functional track are concentrically arranged, and the annular functional track is positioned outside the annular incubation track.

3. The chemiluminescent immunoassay reaction plate with multiple reaction modes of claim 1, wherein the lower part of the circular incubation track is provided with a heating layer, and the heating layer is composed of 7 heating zones.

4. The chemiluminescent immunoassay reaction tray with multiple reaction modes according to claim 1, wherein the annular incubation track is provided with an incubation transmission rack, the annular functional track is provided with a functional transmission rack, the incubation transmission rack and the functional transmission rack are both annular and are provided with more than two reaction cup slots distributed at equal intervals, wherein the reaction cup slots are positioned above the track groove, and the incubation transmission rack and the functional transmission rack are driven by a motor via a gear transmission mechanism to rotate around the center of the rotary table to drive the reaction cups to move along the incubation track and the functional track respectively.

5. The chemiluminescent immunoassay reaction tray of claim 4, wherein the functional transmission rack operates with the following structure: the side surface of the function transmission rack is provided with continuous saw teeth meshed with the gear of the function motor, and the output shaft of the function motor drives the gear of the function motor to rotate so as to continuously drive the function transmission rack to operate.

6. The chemiluminescent immunoassay reaction plate with multiple reaction modes of claim 4, wherein the incubation transmission rack can be driven to rotate by a single incubation transmission motor, or by a gear transmission mechanism driven to rotate by a functional motor by arranging continuous saw teeth on the outer ring of the incubation transmission rack.

7. The chemiluminescent immunoassay reaction plate with multiple reaction modes as claimed in claim 1, wherein two sets of lane-changing gripping mechanisms are provided on the annular turntable for gripping the cuvettes on the incubation track to the functional track and gripping the cuvettes on the functional track to the incubation track respectively.

8. The chemiluminescent immunoassay reaction tray with multiple reaction modes according to claim 1, wherein a loading area, a first sample adding area, a first blending area, a first lane changing hand grip area, a first washing area, a second sample adding area, a second blending area, a second lane changing hand grip area, a second washing area, a third blending area, a measuring area and an unloading area are sequentially arranged on the annular tray body.

9. A chemiluminescent immunoassay analyzer having multiple modes of reaction characterized by an annular disk as claimed in any one of claims 1 to 8.

10. The chemiluminescent immunoassay analyzer having multiple reaction modes of claim 9, wherein the measuring region is provided with a measuring mechanism; the washing district is equipped with the washing mechanism, the mixing district is equipped with the mixing mechanism, the uninstallation district is equipped with consumptive material uninstallation mechanism.

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