CN114643248A - Rotating disc type measuring mechanism, cleaning and measuring device and chemiluminescence immunoassay analyzer - Google Patents
Rotating disc type measuring mechanism, cleaning and measuring device and chemiluminescence immunoassay analyzer Download PDFInfo
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- CN114643248A CN114643248A CN202011518444.6A CN202011518444A CN114643248A CN 114643248 A CN114643248 A CN 114643248A CN 202011518444 A CN202011518444 A CN 202011518444A CN 114643248 A CN114643248 A CN 114643248A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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Abstract
The invention relates to a rotating disc type measuring mechanism and application thereof, comprising: the transfer disc is used for transferring the reaction cups to corresponding operation positions, the operation positions are distributed in the circumferential direction of the transfer disc and at least comprise reaction cup taking and placing positions and light measuring positions, and reaction cup taking and placing position openings are formed in the top wall of the outer shell corresponding to the reaction cup taking and placing positions; the detection mechanism is correspondingly arranged at the light measurement position and is used for detecting the luminous intensity of the reaction cup in the light measurement position; and the shading device is correspondingly arranged at the position of the reaction cup taking and placing port and can completely shield or open the reaction cup taking and placing port. The invention realizes the measurement of the luminous intensity on the transfer disc, and the transfer disc is completely formed into a closed whole through the shading device, thereby avoiding the external light from entering the measurement position to the maximum extent and ensuring the accuracy of the measurement result.
Description
Technical Field
The invention relates to the field of in-vitro diagnosis, measurement and cleaning, in particular to a rotating disc type measuring mechanism, a cleaning and measuring device and a chemiluminescence immunoassay analyzer.
Background
A chemiluminescence immunoassay analyzer is an analysis method established by combining luminescence analysis and immunoreaction, and magnetic particle chemiluminescence is adopted at present, namely a sample to be detected and magnetic particles are added into a reaction cup for reaction, then magnetic separation and cleaning are carried out to clean other interference substances except the magnetic particles and target analytes in the reaction cup, and enzymatic chemiluminescence substrates (acid excitation liquid and alkaline excitation liquid) are added for measurement.
Among the prior art, chemiluminescence immunoassay appearance measurement function realizes setting up measurement mechanism alone or realizing on hatching the dish, and these two kinds all need lean on additional arm to move the reaction cup to corresponding operation position, and the integrated level is low, and the structure is numerous and diverse, and is bulky.
Disclosure of Invention
The invention aims to provide a rotating disc type measuring mechanism which is high in measuring accuracy and simplifies a sample analysis structure.
The technical scheme adopted by the invention is as follows: a rotating disc measuring mechanism comprising:
the bottom plate is provided with an outer shell;
the transfer disc is arranged in the outer shell and used for transferring the reaction cups to corresponding operation positions, the operation positions are distributed in the circumferential direction of the transfer disc and at least comprise reaction cup taking and placing positions and light measuring positions, and reaction cup taking and placing position openings are formed in the top wall of the outer shell corresponding to the reaction cup taking and placing positions;
the detection mechanism is correspondingly arranged at the light measurement position and is used for detecting the luminous intensity of the reaction cup in the light measurement position;
and the shading device is correspondingly arranged at the position of the reaction cup taking and placing port and can completely shield or open the reaction cup taking and placing port.
Further, the shade device includes:
the light shielding sheet rotates to completely shield or open the reaction cup taking and placing opening;
and the driving unit drives the shading sheet to rotate.
Further, the shade device further includes:
the coded disc is driven by the driving unit and synchronously rotates with the light shielding sheet, and a notch is formed in the outer edge of the coded disc;
the first detection optocoupler is arranged at the outer edge of the coded disc, and when the first detection optocoupler is shielded by a flange on one side of the notch, the anti-dazzling screen completely shields or opens the reaction cup taking and placing opening.
Furthermore, a light measuring through hole is formed in the side wall of the shell, and the detection mechanism is installed at the light measuring through hole and passes through the light measuring through hole to detect the luminous intensity of the reaction cup in the light measuring position.
The invention also provides a cleaning and measuring device which comprises the rotating disc type measuring device.
The invention also provides a chemiluminescence immunoassay analyzer which comprises the rotating disc type measuring mechanism or the cleaning measuring device.
The light-shading device has the advantages that the light-emitting intensity on the transfer disc is measured, the transfer disc is completely formed into a closed whole through the light-shading device, external light is prevented from entering a light-measuring position to the maximum extent, and the accuracy of a measuring result is guaranteed.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious to those skilled in the art that other drawings can be obtained according to the drawings without any inventive exercise.
FIG. 1 is a schematic view of a completely shielding structure of a reaction cup pick-and-place opening of a cleaning and measuring device;
FIG. 2 is a schematic view of a completely open structure of a reaction cup pick-and-place opening of a cleaning and measuring device;
FIG. 3 is a cross-sectional view of a cleaning measuring device;
FIG. 4 is a schematic view of a light shielding device;
FIG. 5 is a schematic view of the structure of the kneading mechanism;
FIG. 6 is a cross-sectional view of the homogenizing mechanism;
FIG. 7 is a bottom view of the kneading mechanism;
1-a bottom plate; 2-an outer shell; 3-a transfer tray; 4-a detection mechanism; 5-taking and placing position openings of the reaction cups; 6-shading device, 7-liquid injection mechanism; 8-a liquid discharge mechanism; 9-a blending device; 10-a reaction cup; 11-an acid addition mechanism; 12-an alkali adding mechanism; 101-a lifting motor; 102-a screw rod; 103-a lifting plate; 104-a guide mechanism; 201-a base; 202-a top cover; 203-rolling bearings; 204-a gear disc; 61-shading sheet; 62-a drive unit; 63-code disc; 64-detecting the optical coupler I; 901-eccentric wheel; 902-a drive mechanism; 903-mounting the housing; 904-a vibration seat; 905-mounting holes; 906-card slot; 907-optical coupling sheet; 908-detecting optocoupler two.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the specific embodiments of the present invention and the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention. The technical solutions provided by the embodiments of the present invention are described in detail below with reference to the accompanying drawings.
Example 1
Referring to fig. 1-2, a turntable type measuring mechanism includes: the device comprises a bottom plate 1, an outer shell 2, a transfer disc 3, a detection mechanism 4 and a shading device 6;
the bottom plate 1 is provided with an outer shell;
the transfer disc 3 is arranged in the outer shell 2 and used for transferring the reaction cups to corresponding operation positions, the operation positions are distributed in the circumferential direction of the transfer disc and at least comprise reaction cup taking and placing positions and light measuring positions; the operation position can be all operation steps required before measurement, including sample addition, reagent addition, cleaning and even mixing and the like.
As shown in fig. 3, the outer casing 2 is made of opaque material and is used for enclosing the transfer tray 3 therein, in this embodiment, as shown in fig. 3, the outer casing includes a base 201 disposed on a bottom plate and a top cover 202 covering the base, a hollow cavity is disposed on the upper portion of the base, the transfer tray 3 is disposed in the hollow cavity of the base 201, one end of a rotating shaft passes through the base and is connected to a gear tray 204, the gear tray 204 is connected to a motor through a belt, the other end passes through the base 201 and is connected to the transfer tray 3, the rotating shaft is connected to the base 201 through a rolling bearing 203, and the transfer tray has a plurality of reaction cup placing holes; when the motor rotates, the base is fixed, and the motor rotates to drive the transfer disc 3 to transfer the reaction cup to a corresponding operation position.
The detection mechanism 4 is correspondingly arranged at the light measurement position and is used for detecting the luminous intensity of the reaction cups in the light measurement position, and a reaction cup taking and placing position opening 5 is also arranged at the position, corresponding to the reaction cup taking and placing position, of the top wall of the outer shell 2; the utility model discloses a light measuring device, including shell, detection mechanism 4, photometry through-hole is seted up on the shell lateral wall, detection mechanism 4 install in photometry through-hole department and pass through it is right to photometry through-hole the luminous intensity of reaction cup detects in the photometry position.
And the shading device 6 is correspondingly arranged at the position of the reaction cup taking and placing port and can completely shield or open the reaction cup taking and placing port.
Specifically, as shown in fig. 4, the shade 6 includes: a light shielding sheet 61, a driving unit 62, a code wheel 63 and a first detection optical coupler 64.
The light shielding sheet 61 rotates to completely shield or open the reaction cup taking and placing opening; the specific shape of the light-shielding sheet 61 is not limited, and may be square, triangular or fan-shaped, and in the present embodiment, as shown in fig. 1, the shape of the light-shielding sheet is fan-shaped.
The driving unit 62 drives the light shielding sheet 61 to rotate, the specific structure of the driving unit 62 is not limited, and the driving unit can be an existing stepping motor as long as the driving unit can drive the light shielding sheet 61 to rotate forward and backward.
The coded disc 63 is driven by the driving unit 62 and rotates synchronously with the light shielding sheet 61, and a notch 65 is formed in the outer edge of the coded disc; the coded disc 63 and the light shielding sheet 61 are fixed on the rotating shaft of the stepping motor.
The first detection optocoupler 64 is arranged at the outer edge of the coded disc 63, the first detection optocoupler 64 detects the rotation angle of the coded disc 63 according to whether the coded disc is reset to the first detection optocoupler, namely the rotation angle of the stepping motor, when the first detection optocoupler 64 is shielded by a flange at one side of the notch 65, the coded disc is successfully reset, and when the first detection optocoupler is shielded by a flange at one side of the notch, the light shielding sheet can be in a state of completely shielding or opening the access opening of the reaction cup; in this embodiment, when the detection optocoupler one 64 is shielded by the rib on one side of the notch 65, it indicates that the coded disc is successfully reset, and at this time, the light shielding sheet 61 completely opens the reaction cup taking and placing port 5; when measurement is needed, in order to form a completely closed measurement environment, the stepping motor rotates by a preset angle, the light shielding sheet 61 completely shields the reaction cup taking and placing opening 5, after the measurement is finished, the stepping motor rotates reversely by the same angle, and the light shielding sheet 61 completely opens the reaction cup taking and placing opening 5.
Example 2
A wash measuring device, as in fig. 1-3, including foretell carousel formula measuring device, the operation position still includes washs the position, adds sour position and adds the alkali level, it includes a plurality of notes liquid levels and a plurality of liquid levels of arranging to wash the position, a plurality of notes liquid levels with a plurality of liquid levels of arranging are located the reaction cup and are got to put between the position and the measurement position, add sour position with add the alkali level in proper order be located wash the position with measure between the position, wash measuring device still includes:
a magnetic member disposed at a side of a transfer path of the transfer tray; the specific magnetic part is arranged on the inner side or the outer side of a transfer path of the transfer disc according to the cleaning effect, so that the magnetic beads of the reaction cup are respectively gathered on the corresponding operation positions to one side of the magnetic part, and the magnetic beads are fully scattered and effectively cleaned in the transfer process of the reaction cup.
The liquid injection mechanism 7 is correspondingly arranged at the liquid injection level and is used for injecting cleaning liquid into the reaction cup at the liquid injection level; the liquid injection mechanism 7 is fixed at the top cover liquid injection level;
the liquid discharge mechanism 8 is correspondingly arranged at the liquid discharge level and is used for discharging the liquid in the reaction cup at the liquid discharge level, and the liquid discharge mechanism 8 is driven by the lifting mechanism to vertically lift so as to discharge the liquid in the reaction cup at the liquid discharge level;
the acid adding mechanism 11 is correspondingly disposed at the acid adding position, and the specific structure is not limited as long as the acidic excitation liquid can be injected into the reaction cup at the acid adding position.
The alkali adding mechanism 12 is correspondingly disposed at the alkali adding position, and the specific structure is not limited as long as the alkali exciting liquid can be injected into the reaction cup at the alkali adding position.
In this embodiment, the acid adding mechanism 11 and the alkali adding mechanism 12 are respectively fixed at the acid adding position and the alkali adding position of the top cover.
It is specific, wash the position and include three notes liquid level and three row liquid level, three notes liquid level and three flowing back are located the reaction cup and get put the position and add between the acid level and along transporting a set circumference distribution in turn, annotate liquid mechanism 7 and include three notes liquid needle, it is three annotate the liquid needle respectively with three it corresponds the setting to annotate the liquid position, drainage mechanism 8 includes three row liquid needle, three row liquid needle respectively with the flowing back position corresponds the setting.
Elevating system install in transport a set top, including elevator motor 101, lead screw 102 and lifter plate 103, the drainage needle all be fixed in on the lifter plate, lead screw 102 upper end links to each other with elevator motor 101, elevator motor 101 drives lead screw 102 and rotates, and then realizes the vertical lift motion of liquid injection needle.
Further, the lifting mechanism 10 further comprises two guide mechanisms 104 which are symmetrically arranged, so that the lifting of the lifting plate is more stably realized, the guide mechanism 10 comprises a guide shaft and a guide shaft sleeve, the lower part of the guide shaft is fixed with the top cover, and the upper part of the guide shaft is fixed with the lifting plate through the guide shaft sleeve.
The invention integrates the magnetic bead cleaning, separating and measuring into a whole, is realized on a transfer disc, and has high integrated automation degree and small volume.
In order to better promote the reaction, the rotating disc type measuring mechanism further comprises a blending device 9 which is correspondingly arranged at the acid adding position and used for blending the reaction cups in the acid adding position.
Specifically, as shown in fig. 4 to 7, the blending device 9 includes: an eccentric wheel 901, a driving mechanism 902, a mounting shell 903 and a vibration seat 904;
the driving mechanism 902 drives the eccentric wheel 901 to rotate; the structure of the driving mechanism 902 may not be limited, in this embodiment, the driving mechanism 902 is a driving motor, and a rotating shaft of the driving motor is connected with the eccentric wheel 901;
the mounting shell 903 is fixed on the bottom plate 1, and the mounting shell 903 and the bottom plate 1 form a limiting frame;
one end of the oscillating base 904 is provided with a mounting hole 905 for accommodating the eccentric wheel 901; the other end of the oscillation seat 904 penetrates through the limiting frame and is provided with a clamping groove 906, the clamping groove 906 extends into the outer shell 2, the lower portion of the reaction cup 10 in the acid adding position is inserted into the clamping groove 906, a gap is reserved between the side wall of the clamping groove 906, the eccentric wheel 901 can drive the oscillation seat 904 to reciprocate in the limiting frame 906 when rotating in the mounting hole 905, and when the oscillation seat 904 reciprocates, the side walls on two sides of the clamping groove 906 can impact the reaction cup.
The specific structure of the mounting case 903 is not limited as long as a limiting frame can be formed with the bottom plate 1; the oscillating seat is limited up, down, front and back, and only the oscillating seat is allowed to move left and right;
in the embodiment provided by the invention, the oscillation seat 904 penetrates through the limiting frame, one end with a mounting hole 905 is positioned in the mounting shell 906, the other end with a clamping groove 906 extends out of the mounting shell 903, the external dimension of the middle section of the oscillation seat is the same as the dimension of the limiting frame, the oscillation seat 904 is limited up and down and back and forth, and only the oscillation seat 904 is allowed to reciprocate left and right;
further, in order to more stably realize the reciprocating motion of the oscillating base 904, in this embodiment, the mounting hole 905 is disposed in the center of the end of the oscillating base 904, the mounting hole 905 is shaped like a kidney, the width of the kidney-shaped mounting hole is the same as the diameter of the eccentric 901, and the width of the clamping groove is not greater than 2 times the distance from the rotation point of the eccentric to the central point.
When the reaction cup works, when the eccentric wheel 901 drives the vibration seat 904 to move rightwards in the limiting frame 906, the left side wall of the clamping groove 906 can impact the reaction cup, and when the eccentric wheel drives the vibration seat 904 to move leftwards in the limiting frame 906, the right side wall of the clamping groove 906 can impact the reaction cup, so that liquid in the reaction cup forms turbulent flow, and the reaction of the liquid in the reaction cup is promoted.
The blending device further comprises a light coupling piece 907 and a second detection optocoupler 908, the second detection optocoupler 908 is connected to the end wall of the oscillating base 904 with the mounting hole, and the second detection optocoupler 908 is used for detecting whether the oscillating base 904 impacts the reaction cup according to whether the light coupling piece 907 resets to the second detection optocoupler 908.
The reaction cup 10 is placed in the reaction cup taking and placing position of the transfer disc 3 through the reaction cup taking and placing opening 5, and a liquid injection needle injects cleaning liquid into the reaction cup along with the rotation of the transfer disc 3 to the liquid injection level, so that the cleaning of the reaction cup is realized; the reaction cup 10 continues to rotate along with the transfer disc 3 to reach a liquid discharge level, and the liquid discharge needle vertically moves downwards to be inserted into the reaction cup to suck the liquid in the reaction cup; the primary liquid beating and absorbing is primary cleaning; repeating the liquid beating and liquid suction for three times to realize three times of cleaning; after the three times of cleaning, the reaction cup rotates along with the transfer disc 3 to add an acid position, acid excitation liquid is injected into the reaction cup, the mixing mechanism mixes the reaction cup at the moment, the reaction cup rotates along with the transfer disc 3 to an alkali adding position, then alkaline excitation liquid is injected into the reaction cup, meanwhile, the shading mechanism completely shades the shading port, and the detection mechanism starts to measure light.
Example 3
A chemiluminescence immunoassay analyzer comprises the cleaning and measuring device.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A carousel formula measuring mechanism which characterized in that includes:
the transfer disc is used for transferring the reaction cups to corresponding operation positions, the operation positions are distributed in the circumferential direction of the transfer disc and at least comprise reaction cup taking and placing positions and light measuring positions;
the transfer disc is arranged in the outer shell, and a reaction cup taking and placing position opening is formed in the top wall of the outer shell, corresponding to the reaction cup taking and placing position;
the detection mechanism is correspondingly arranged at the light measurement position and is used for detecting the luminous intensity of the reaction cup in the light measurement position;
and the shading device is correspondingly arranged at the position of the reaction cup taking and placing port and can completely shield or open the reaction cup taking and placing port.
2. The rotating disc measuring mechanism of claim 1, wherein the light shield comprises:
the light shielding sheet rotates to completely shield or open the reaction cup taking and placing opening;
and the driving unit drives the shading sheet to rotate.
3. The rotating disc measuring mechanism of claim 2, wherein the light shield further comprises:
the coded disc is driven by the driving unit and synchronously rotates with the light shielding sheet, and a notch is formed in the outer edge of the coded disc;
the first detection optocoupler is arranged at the outer edge of the coded disc, and when the first detection optocoupler is shielded by a flange on one side of the notch, the shading sheet completely shields or opens the reaction cup taking and placing opening.
4. The rotating disc type measuring mechanism according to claim 1, wherein a light measuring through hole is formed on a side wall of the housing, and the detecting mechanism is installed at the light measuring through hole and detects the light intensity of the reaction cup in the light measuring position through the light measuring through hole.
5. A cleaning and measuring device according to claim 1 and including said carousel-type measuring means.
6. A chemiluminescent immunoassay analyzer comprising the carousel measuring device of any one of claims 1 to 4 or the wash measuring device of claim 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011518444.6A CN114643248A (en) | 2020-12-21 | 2020-12-21 | Rotating disc type measuring mechanism, cleaning and measuring device and chemiluminescence immunoassay analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011518444.6A CN114643248A (en) | 2020-12-21 | 2020-12-21 | Rotating disc type measuring mechanism, cleaning and measuring device and chemiluminescence immunoassay analyzer |
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| Publication Number | Publication Date |
|---|---|
| CN114643248A true CN114643248A (en) | 2022-06-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011518444.6A Pending CN114643248A (en) | 2020-12-21 | 2020-12-21 | Rotating disc type measuring mechanism, cleaning and measuring device and chemiluminescence immunoassay analyzer |
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| CN (1) | CN114643248A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116786504A (en) * | 2023-08-29 | 2023-09-22 | 江苏三联生物工程股份有限公司 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
-
2020
- 2020-12-21 CN CN202011518444.6A patent/CN114643248A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116786504A (en) * | 2023-08-29 | 2023-09-22 | 江苏三联生物工程股份有限公司 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
| CN116786504B (en) * | 2023-08-29 | 2023-11-03 | 江苏三联生物工程股份有限公司 | Magnetic bead cleaning and separating device and method for chemiluminescent immunoassay analyzer |
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