CN116688020A

CN116688020A - Pharmaceutical composition for treating wind-heat type common cold and preparation method thereof

Info

Publication number: CN116688020A
Application number: CN202310716570.XA
Authority: CN
Inventors: 蓝晓步; 卢兴; 秦艳娥; 刘平亮; 黄仕孙; 植国繁; 王佑文
Original assignee: First Peoples Hospital of Nanning
Current assignee: First Peoples Hospital of Nanning
Priority date: 2023-06-16
Filing date: 2023-06-16
Publication date: 2023-09-05

Abstract

The invention discloses a pharmaceutical composition for treating wind-heat type common cold and a preparation method thereof, wherein the pharmaceutical composition comprises the following components in parts by mass: 5-15 parts of Japanese dock root bark, 20-30 parts of trigeminal bitter, 20-30 parts of roughhaired holly root, 8-12 parts of hickory nut and 5-9 parts of cordate houttuynia. Extracting under heating by mixing with water, filtering, concentrating, standing, filtering to remove precipitate, adding appropriate amount of pure water, adding sucrose 300g, glycyrrhrizae radix fluid extract 25ml, mentholum 0.3g, benzoic acid 0.5g, and ethylparaben 0.5g into each 1000ml of medicinal liquid, boiling, stirring, and sterilizing. Can be used for treating wind-heat type common cold, and has the advantages of small side effect, convenient administration, rapid drug effect and good anti-inflammatory effect.

Description

Pharmaceutical composition for treating wind-heat type common cold and preparation method thereof

Technical Field

The invention belongs to the technical field of medicines, and in particular relates to a pharmaceutical composition for treating wind-heat type common cold and a preparation method thereof.

Background

Wind-heat cold usually occurs in summer and autumn, and is a superficiality syndrome caused by pathogenic wind-heat invading the exterior and lung qi failing to control. Patients often present with symptoms of fever, slight aversion to wind-cold, sore throat, headache, swelling and pain of tonsils, yellow nasal discharge, nasal obstruction, cough with yellow sputum or sticky sputum, thirst with a desire for drink, superficial and rapid pulse, thin tongue fur Bai Weihuang. At present, a plurality of medicines for treating wind-heat type common cold are available in the market, and all the medicines have the self treatment characteristics. Some therapeutic formulas have strong clearing and descending properties and good antipyretic effect, but are bitter and cold in nature, and are not suitable for patients with weak body or deficiency-cold spleen and stomach, such as children and the elderly, for example, yinqiao Jiedu san; although the granule has small side effects, the granule is prepared into granule by hot water, and has troublesome administration and slow onset of action after administration, such as radix Isatidis granule, GANMAOLING granule, etc. In addition, common cold of patients often accompanies various inflammations, and although most of the existing common cold contains a certain anti-inflammatory component, there is room for improvement in the actual anti-inflammatory effect.

Disclosure of Invention

The invention provides a pharmaceutical composition for treating wind-heat type common cold and a preparation method thereof, which aim to solve the defects in the prior art.

In order to solve the technical problems, the invention adopts the following technical scheme:

the invention provides a pharmaceutical composition for treating wind-heat type common cold, which is prepared from the following components in parts by mass:

5-15 parts of Japanese dock root bark, 20-30 parts of trigeminal bitter, 20-30 parts of roughhaired holly root, 8-12 parts of hickory nut and 5-9 parts of cordate houttuynia.

Preferably, the pharmaceutical composition is prepared from the following components in parts by mass:

10 parts of Japanese dock root bark, 25 parts of trigeminal bitter, 25 parts of roughhaired holly root, 10 parts of Indian screwtree root and 7 parts of heartleaf houttuynia herb.

Of the above-mentioned components,

the bark of Japanese dock root is bark and root bark of Japanese dock root, the Japanese dock root is plant of Centipeda of Araliaceae, the shape is arbor or shrub, the height is 2-15 meters, and the chest diameter can reach more than 30 cm; the branches are thick and strong, wrinkles are formed when the branches are dry, the young branches are dense, star-shaped, short and soft, and the hairs are gradually thinned after a while. Root bark, root and leaf are used as medicines, and can be collected all the year round, and the root bark and root bark are cleaned, sliced and dried in the sun for standby.

The evodia genus plant of Rutaceae family is characterized by evergreen shrub or small arbor, up to 3 m, and bitter taste of whole plant. The bark is grey or grey, smooth and has yellowish pores. The stems are thick and multi-branched. 3, the cotyledon is pairs of leaves, and the length of a petiole is 3-18 cm; the small leaf paper is in a rectangular round needle shape, the length is 6-12 cm, the width is 2-6 cm, the tip end is long, the base part is gradually narrowed to form a short handle, the whole edge or irregular shallow wave shape is provided with glandular spots, and the two sides are smooth and hairless. Taking root and leaf as medicine, collecting all year round, cleaning root, slicing and sun drying; and drying the leaves in the shade.

The holly is characterized in that fallen leaves of shrubs and young branches are scattered with a plurality of white holes, which are similar to the scalene. She Husheng it is made up by using membranous material, oval or messy ellipse, its front end is pointed or sharp, its base portion is circular or wide wedge-shaped, its edge has fine saw tooth flower white or yellowish green, parthenogenesis strain, male flower single body or 2-3 cluster leaf axillary, female flower single body is formed into leaf axillary, sepal, petal and stamen are usually 4, sometimes 5-6; superior to the ovary. The root and leaf are used as medicines, can be collected all the year round, cleaned, sliced and dried for standby.

The plant of the genus Hibiscus of the family Firmiaceae is in the form of small shrubs, about 1 meter higher. The stems are upright and branched, the stem skin is tough and tingling, and the small branches are densely covered with grey yellow short villi. The single leaves are mutually grown, the leaf stems are about half cm long, the leaf stems are round and needle-shaped, the length is 2.5-8 cm, the width is 1.1-3 cm, the tip is slightly pointed or blunt, the base is wide and wedge-shaped, the whole edge is full, the upper surface is nearly free of hair or loose and grown star-shaped soft hair, the lower surface is densely covered by gray yellow star-shaped hair to be in a gray green color, and the main pulse is 3. Extracting the inflorescence peduncles from the armpit of summer leaves, clustering a plurality of flowers on the inflorescence peduncles, and forming a basal part with a narrow-tip bract; calyx tube-shaped, and fur; the corolla is about 8mm long, petals 5 are unequal in length, long round, light purple, the front end is nearly round and slightly concave, and 2 ear-shaped protrusions are arranged near the base; 10 stamens, 5 degenerated stamens, and a column of stamens co-formed with the gynoecia. The plastic fruit is oval and slightly similar to sesame fruits, has a length of approximately 2 cm, is densely covered with star-shaped tricholoma matsutake, and is 5 cracks after being cooked. The root or whole plant can be used as medicine, and can be cleaned, sliced and dried in the sun for later use in summer and Qiu Caiwa.

Herba Houttuyniae is dry aerial part of houttuynia cordata of Saururaceae, and has a shape of flat cylindrical stem, distortion, length of 20-35 cm, and diameter of 0.2-0.3 cm; the surface is brown yellow, has a plurality of longitudinal edges, has obvious sections, and has residual fibrous roots on the lower section; is crisp and easy to break. She Husheng the blades are curled and contracted, flattened and heart-shaped, and have the length of 3-5 cm and the width of 3-4.5 cm; the tip is tapered and the whole edge is formed; the upper surface is dark yellow-green to dark brown, and the lower surface is gray-green or gray-brown; the petiole is slender, and the base and the supporting leaf are combined to form a sheath shape. The spike grows top and is yellow brown. The grating has fishy smell and slightly astringent taste. Can be picked up and cut when the stem and leaf are luxuriant and the flower is rich in ears in summer, the impurities are removed, and the flower is dried in the sun for standby.

The invention also provides a preparation method of the pharmaceutical composition based on the proportion of the raw materials.

The manufacturing method comprises the following steps:

(1) Weighing cortex Momordicae Charantiae, radix Tripterygii Wilfordii, flos Ilicis Purpureae, radix Rhododendri mollis and herba Houttuyniae in proportion;

(2) And (3) weighing the materials in the step (1), mixing, adding water, heating and extracting, filtering the obtained extracting solution, concentrating, standing and filtering out precipitate. Adding pure water, sucrose, glycyrrhrizae radix fluid extract, mentholum, benzoic acid, and ethylparaben into the filtrate, boiling, and stirring.

Compared with the prior art, the invention has the following beneficial effects:

1. the side effect is small, and the toxicity of the medicinal material components is small, so that the medicinal composition prepared by adopting the medicinal material components is also small, has small side effect on human bodies when being used for treating cold, and is suitable for weak people such as children and the elderly;

2. the medicine liquid obtained by the preparation method can be directly taken orally, and the unnecessary trouble of taking with water is reduced.

3. The medicine effect is fast, and the medicine liquid is obtained by the extraction method in the preparation of the medicine, so that the medicine has the advantage of fast medicine effect.

4. The anti-inflammatory effect is better, and in practical application, the anti-inflammatory effect of the pharmaceutical composition is better than that of other cold medicines.

Detailed Description

According to the theory of traditional Chinese medicine, cold and heat are abnormal, warm and cool and lose joints, the cold is not the main cause of cold in the age, wind-heat cold is the pathogenic factor of wind-heat invading lung defenses and the pathogenic factor struggling with each other, so fever is serious, aversion to cold is light, and the symptoms are that the lung and stomach stagnate heat and the warm pathogenic factor are damp. The invention provides a pharmaceutical composition for treating wind-heat type common cold, which is prepared by extracting components of Japanese dock root bark, trigeminal bitter, roughhaired holly root, hickory nut and cordate houttuynia with water.

Wherein, the Japanese dock root Pi Xingliang has sweet taste and can induce sweat, relieve exterior syndrome, dispel wind and remove dampness so as to remove dampness; the three parts of the roughhaired holly root, the roughhaired holly root and the Indian screwtree root have cold property and simultaneously have the effects of clearing heat and detoxicating so as to strengthen the effects of ventilating and dispersing lung and clearing heat. Meanwhile, the radix sophorae flavescentis, the radix clematidis and the radix sesami Indicae can promote the production of body fluid, quench thirst, relieve sore throat and detumescence, and can assist in dispersing dampness and relieving exterior syndrome so as to penetrate through membranogen. Herba Houttuyniae is pungent and cold in nature, has the effects of clearing heat and detoxicating, resolving carbuncles and expelling pus, and inducing diuresis and treating stranguria, and the decanoyl acetaldehyde component contained in the herba Houttuyniae has definite antibacterial and antiviral effects. In addition, licorice or licorice fluid extract may be added, licorice has the neutralizing effect and the effect of regulating the functions of the other drugs, so that the heat-relieving herbs can relieve the heat, the cold-relieving herbs can relieve the cold, and the cold-heat phase can be smoothed, so that it has the auxiliary effect. All the medicines in the whole formula have the effects of relieving exterior syndrome, eliminating pathogenic factors, relieving sore throat, dispelling wind, removing dampness, detoxifying and strengthening body resistance.

The local wind-heat cold patients in the south are moist in climate, and often accompanied with symptoms of no rising of body heat and tiredness of spirit and tiredness of damp-heat, and the duck foot bark has the effects of dispelling wind and removing dampness, so the traditional Chinese medicine composition is more suitable for the wind-heat cold patients in the south compared with other cold medicines.

The technical solution of the present invention is further described below with reference to specific embodiments, and it should be understood that the specific embodiments described herein are only for explaining the present invention and are not limiting the present invention.

Example 1:

the medicine composition for treating wind-heat type common cold is prepared from the following components in parts by mass: 5 parts of Japanese dock root bark, 20 parts of trigeminal bitter, 20 parts of roughhaired holly root, 8 parts of Indian screwtree root and 5 parts of heartleaf houttuynia herb.

The preparation method of the pharmaceutical composition for treating wind-heat type common cold comprises the following steps:

(1) Weighing the bark of the Japanese dock root, the trigeminal bitter, the holly bark, the wild sesame and the cordate houttuynia in proportion, specifically, in the embodiment, weighing 50g of the Japanese dock root bark, 200g of the trigeminal bitter, 200g of the holly bark, 80g of the wild sesame and 50g of the cordate houttuynia;

(2) Heating the medicine materials weighed in the step (1) with water, decocting for two times, wherein the weight of each water is 4 times of the weight of the medicine materials weighed, the time of each decoction is 2 hours, mixing the two decoctions, and filtering;

concentrating the obtained filtrate to 700mL, standing for 24 hours, and filtering out precipitate;

adding a proper amount of pure water into the obtained filtrate to obtain 1000mL of liquid medicine, adding 300g of sucrose, 25mL of liquorice fluid extract, 0.3g of menthol, 0.5g of benzoic acid and 0.5g of ethylparaben, wherein the sucrose is directly added or the sucrose is boiled into 70% syrup and then added;

boiling the liquid medicine for 10 minutes, uniformly stirring the liquid medicine, and detecting intermediate in the process, wherein the detection indexes comprise relative density, the relative density value is 1.04-1.1, and the liquid medicine is characterized by brown liquid, slightly cool gas, bitter taste and slightly sweet;

and finally, sub-packaging the liquid medicine, and sterilizing the liquid medicine.

Example 2:

the medicine composition for treating wind-heat type common cold is prepared from the following components in parts by mass: 15 parts of Japanese dock root bark, 30 parts of trigeminal bitter, 30 parts of roughhaired holly root, 12 parts of Indian screwtree root and 9 parts of heartleaf houttuynia herb.

(1) Weighing the bark of the Japanese dock root, the trigeminal bitter, the holly bark, the wild sesame and the cordate houttuynia in proportion, specifically, in the embodiment, 150g of the Japanese dock root bark, 300g of the trigeminal bitter, 300g of the holly bark, 120g of the wild sesame and 90g of the cordate houttuynia;

(2) Decocting the materials in water for 2 hr, mixing the decoctions, and filtering;

concentrating the filtrate to 700mL, standing for 24 hours, and filtering out precipitate;

adding a proper amount of pure water into the obtained filtrate to obtain 1000mL of liquid medicine, and adding 300g of sucrose, 25mL of licorice fluid extract, 0.3g of menthol, 0.5g of benzoic acid and 0.5g of ethylparaben;

boiling for 10 minutes, uniformly stirring the liquid medicine, and detecting intermediate in the process, wherein the detection indexes comprise relative density, the relative density value is 1.04-1.1, and the liquid medicine is brown liquid, slightly cool, bitter and slightly sweet;

Example 3:

the medicine composition for treating wind-heat type common cold is prepared from the following components in parts by mass: 10 parts of Japanese dock root bark, 25 parts of trigeminal bitter, 25 parts of roughhaired holly root, 10 parts of Indian screwtree root and 7 parts of heartleaf houttuynia herb.

(1) Weighing the bark of the Japanese dock root, the trigeminal bitter, the holly bark, the wild sesame and the cordate houttuynia in proportion, specifically, in the embodiment, weighing 100kg of the bark of the Japanese dock root, 250kg of the trigeminal bitter, 250kg of the holly bark, 100kg of the wild sesame and 70kg of the cordate houttuynia;

(2) Mixing the medicinal materials, placing into an extraction tank, adding water, heating under normal pressure, reflux extracting twice, wherein the weight of each water is 5 times of the weight of the weighed medicinal materials, mixing the two extractive solutions, and soaking the medicinal materials in water for 2 hours before extraction to ensure that the medicinal materials can fully contact with water;

filtering with 100 mesh duplex filter, pumping filtrate into double-effect concentrator, concentrating filtrate to 700L with the double-effect concentrator at 75deg.C for one-effect concentration and 58 deg.C for two-effect concentration, standing for 24 hr, filtering with frame plate machine or centrifuge, and filtering to remove precipitate;

adding proper amount of pure water into the obtained filtrate to obtain 1000L of medicinal liquid, and adding 300kg of sucrose, 25L of Glycyrrhrizae radix fluid extract, 0.3kg of menthol, 0.5kg of benzoic acid and 0.5kg of ethylparaben;

boiling for 10 minutes, uniformly stirring the liquid medicine, and detecting intermediate, wherein the detection indexes comprise relative density, the relative density value is 1.04-1.1, and the liquid medicine is brown liquid, slightly cool, bitter and slightly sweet;

bottling the liquid medicine by using an oral liquid pot bundling machine, filling 100mL of the liquid medicine into each bottle, placing the filled liquid medicine into a sterilizing cabinet, introducing steam into the sterilizing cabinet for sterilizing for 1 hour, and finally printing a label and pasting the label outside the medicine bottle.

Example 4:

the medicine composition for treating wind-heat type common cold is prepared from the following components in parts by mass: 5 parts of Japanese dock root bark, 25 parts of trigeminal bitter, 30 parts of roughhaired holly root, 12 parts of Indian screwtree root and 7 parts of heartleaf houttuynia herb.

(1) Weighing the bark of the Japanese dock root, the trigeminal bitter, the holly bark, the wild sesame and the cordate houttuynia in proportion, specifically, in the embodiment, weighing 50kg of the Japanese dock root, 250kg of the trigeminal bitter, 300kg of the holly bark, 120kg of the wild sesame and 70kg of the cordate houttuynia;

(2) Mixing the medicinal materials, placing into an extraction tank, adding water, heating under normal pressure, reflux extracting twice, wherein the weight of each water is 4 times of the weight of the weighed medicinal materials, mixing the two extractive solutions, and soaking the medicinal materials in water for 2 hours before extraction to ensure that the medicinal materials can fully contact with water;

filtering with 100 mesh duplex filter, pumping filtrate into double-effect concentrator, concentrating filtrate to 700L with the double-effect concentrator at 83 deg.C for two-effect concentration at 68 deg.C, standing for 24 hr, filtering with frame plate machine or centrifuge, and filtering to remove precipitate;

Example 5:

the medicine composition for treating wind-heat type common cold is prepared from the following components in parts by mass: 15 parts of Japanese dock root bark, 20 parts of trigeminal bitter, 20 parts of roughhaired holly root, 8 parts of Indian screwtree root and 9 parts of heartleaf houttuynia herb.

(1) Weighing the Japanese dock root bark, the trigeminal bitter, the holly bark, the wild sesame and the cordate houttuynia in proportion, specifically, in the embodiment, 150kg of the Japanese dock root bark, 200kg of the trigeminal bitter, 200kg of the holly bark, 80kg of the wild sesame and 90kg of the cordate houttuynia;

(2) Mixing the medicinal materials, placing into an extraction tank, adding water, heating under normal pressure, reflux extracting twice, wherein the weight of each water is 6 times of the weight of the weighed medicinal materials, mixing the two extractive solutions, and soaking the medicinal materials with water for 2 hours before extraction to ensure that the medicinal materials can fully contact water;

filtering with 100 mesh duplex filter, pumping filtrate into double-effect concentrator, concentrating filtrate to 700L with the double-effect concentrator at 78deg.C for two-effect concentration at 63deg.C, standing for 24 hr, filtering with frame plate machine or centrifuge, and filtering to remove precipitate;

Example 6:

the medicine composition for treating wind-heat type common cold is prepared from the following components in parts by mass: 10 parts of Japanese dock root bark, 30 parts of trigeminal bitter, 25 parts of roughhaired holly root, 10 parts of Indian screwtree root and 5 parts of heartleaf houttuynia herb.

(1) Weighing the bark of the Japanese dock root, the trigeminal bitter, the holly bark, the wild sesame and the cordate houttuynia in proportion, specifically, in the embodiment, weighing 100kg of the bark of the Japanese dock root, 300kg of the trigeminal bitter, 250kg of the holly bark, 100kg of the wild sesame and 50kg of the cordate houttuynia;

filtering with 100 mesh duplex filter, pumping filtrate into double-effect concentrator, concentrating filtrate to 700L with the temperature of first-effect concentration of 80deg.C and second-effect concentration of 65deg.C, standing for 24 hr, filtering with frame plate machine or centrifuge, and filtering to remove precipitate;

The effect of the pharmaceutical composition for treating wind-heat type common cold in the present invention is demonstrated by animal experiments as follows.

Test example:

1. a subject:

50 KM mice in the university of Guangxi medical science animal experiment center are in half of the male and female animals.

2. Drug and reagent:

pharmaceutical compositions of the invention, dexamethasone.

3. Test protocol:

randomly dividing a model control group, a dexamethasone control group (the administration dosage concentration is 0.2mL/10 g), a high-dose group of the pharmaceutical composition of the invention (the administration dosage concentration is 0.4mL/10 g), a medium-dose group of the pharmaceutical composition of the invention (the administration dosage concentration is 0.2mL/10 g) and a low-dose group of the pharmaceutical composition of the invention (the administration dosage concentration is 0.1mL/10 g) according to the weight and the sex, wherein the dexamethasone control group is taken as a positive group;

the mice in the pharmaceutical composition group of the invention are continuously irrigated with stomach for one week, irrigated with stomach once a day, and the dosage of the pharmaceutical composition group is 20mL according to the concentration, the model group and the dexamethasone control group are given with the same physiological saline, and finally the dexamethasone control group is injected with 20mL of dexamethasone according to the concentration;

3.1 swelling of the auricle of mice caused by croton oil

After the last administration for 30min, smearing the soybean oil on both sides of the right ear of the mouse to cause acute inflammation, wherein the left ear is not treated to be non-inflammatory;

4h later, killing the mice, cutting two ears along auricles, chiseling out the same-sized lugs at the same part by using an 8mm diameter puncher, and analyzing and comparing the differences among groups by using the weight of an electronic balance;

wherein ear swelling = right ear weight-left ear weight;

ear swelling inhibition = (average ear swelling degree of model group-average ear swelling degree of dosing group)/average ear swelling degree of model group x 100%;

3.2 swelling of the toe of mice caused by carrageenan

After 30min of last dose, 25 μl of 2% carrageenan was injected into the right toe of the mice to cause inflammation, and the left foot was not treated as a non-inflammatory foot;

4 hours later, the mice are sacrificed, the left and right feet are cut off along the ankle joints, and then the weight of the electronic balance is used for analyzing and comparing the differences among groups;

wherein, foot swelling = right foot weight-left foot weight;

foot swelling inhibition = (average foot swelling degree of model group-average foot swelling degree of dosing group)/average foot swelling degree of model group x 100%;

3.3 detection of inflammatory influencing factors in serum

After the last administration for 30min, local inflammation is carried out on the mice;

killing mice after 4 hours, taking out the whole lung, homogenizing in a homogenizer according to the weight (g) of the lung to normal saline (ml) =1:9, centrifuging (4 ℃,3000rpm/min,30 min) in a test tube after homogenizing, and sucking the supernatant to be used in a refrigerator at-20 ℃; the double antibody sandwich ABC-ELISA method is adopted, the detection procedure is carried out according to the instruction of a kit, and the contents of lung tissues and serum inflammatory factors TNF-alpha, IL-1 beta, IL-6, IL-10, SOD and MDA of each group of mice are measured.

4. Test record:

the auricle swelling degree of the model group of 4.1 is 11.87 & gt 10mg, which shows that the molding of the auricle swelling caused by the babble oil is successful. Wherein, the swelling inhibition rate of the dexamethasone group reaches 84.30%; the swelling inhibition rates of the high, medium and low dose groups of the pharmaceutical composition are 19.88%, 10.86% and 2.43% respectively. Compared with the model group, the dexamethasone group can obviously inhibit auricle swelling degree, and the difference has statistical significance (P < 0.05). The difference of the high, medium and low dose groups of the pharmaceutical composition has no statistical significance (P is more than 0.05), but the swelling inhibition rate of auricles increases with the increase of the dose;

effect of different drugs on the auricle swelling degree of KM mice

Note that: comparison to model set: * P <0.05.

4.2 toe swelling of the model group was 44.09mg by measuring toe weight, showing successful molding of carrageenan-induced toe swelling. Wherein, the swelling inhibition rate of the positive group reaches 67.78%; the swelling inhibition rates of the high, medium and low dose groups of the pharmaceutical composition are 34.48%, 23.30% and 21.49%, respectively. Compared with a model group, the dexamethasone group can obviously reduce toe swelling degree in high, medium and low dosage groups, and the difference has statistical significance (P is less than 0.05), so that the result shows that the pharmaceutical composition has anti-inflammatory effect and is in dose-effect relationship;

effect of different drugs on the degree of toe swelling in KM mice

Note that: the numbers indicate that the differences between the group and the model group are statistically significant (P < 0.05)

4.3 compared with model group, dexamethasone group value is lower than under detection line, which shows that its content is obviously lower than that of model group (P < 0.05), and the medicine composition has no obvious effect on every administration group, and the difference has no statistical significance (P > 0.05). The medicine composition has no obvious effect on the serum INF-alpha;

effect of different drugs on TNF- α content in KM mice

Note that: comparison to model set: * P <0.05.

4.4 except the model group, the IL-1 beta content measured by the dexamethasone group and the high-low dosage group of the pharmaceutical composition in the invention is lower than the lowest concentration of an ELISA standard curve measured under the same experimental condition, which shows that each administration group possibly has an inhibiting effect on serum IL-1 beta inflammatory factors;

effect of different drugs on the IL-1 beta content of KM mice

Note that: comparison to model set: * P <0.05.

4.5 compared with the model group, the serum IL-6 content of the dexamethasone positive group and the high-low dose group of the pharmaceutical composition is obviously reduced, and the difference has statistical significance (P < 0.05). The result shows that the pharmaceutical composition has obvious down-regulating effect on IL-6 level of inflammatory mice;

effect of different drugs on the IL-6 content of KM mice

Note that: comparison to model set: * P <0.05.

4.6, compared with the model group, each administration group has no obvious effect on the IL-10 content, is statistically meaningless (P > 0.05), and shows that each administration group has no obvious regulation effect on the IL-10 level of inflammatory mice;

effect of different drugs on KM mouse IL-10

Note that: comparison to model set: * P <0.05.

4.7 compared with the model group, the administration group has no obvious effect on the serum SOD content of inflammatory mice and has no statistical significance (P is more than 0.05);

influence of different drugs on mouse superoxide dismutase (SOD)

Note that: comparison to model set: * P <0.05.

4.8 compared with the model group, each administration group can obviously reduce MDA content, the difference has statistical significance (P is less than 0.05), and the result shows that the pharmaceutical composition has a certain antioxidation effect;

influence of different drugs on the Malondialdehyde (MDA) content of mice

Note that: comparison to model set: * P <0.05.

5. Conclusion of the test:

the experiment adopts a model that croton oil stimulates auricles of mice and carrageenan is injected into toes of the mice to cause local swelling of the mice to study the anti-inflammatory effect of the medicine, and the result shows that the medicine composition has a certain anti-inflammatory effect. The detection of each factor is carried out by a method of taking materials after one week of administration and 4 hours of local inflammatory stimulation, and the result of the experiment shows that the pharmaceutical composition can obviously inhibit the expression of IL-6 and IL-1 beta and has no obvious inhibition effect on INF-alpha and IL-10; the pharmaceutical composition of the invention can increase SOD content to different degrees (the difference has no statistical significance P >0.05 compared with a model group) but can obviously reduce MDA content. The test results show that the pharmaceutical composition has a certain anti-inflammatory effect, and the action mechanism is possibly related to inhibiting the expression of IL-6 and IL-1 beta and reducing the MDA content.

The test result also shows that the positive control medicament dexamethasone has no obvious inhibition effect on IL-10 and can not improve the SOD content. The possible reasons are that the levels of inflammatory factors are different in different phases of the inflammatory response, and the time of blood withdrawal in mice may be a phase in which the expression levels of inflammatory factors are not significantly changed.

Typical cases:

case 1: wang Mou female, 65 years old, abnormal thyroid function examination, upper respiratory tract infection (common cold). Headache due to common cold and limb weakness. The prescription prescribed by doctors contains cold medicines, wherein the cold medicines are the medicine composition in the embodiment, 20mL of the medicine composition is taken three times a day, after 2 days of taking, headache symptoms are improved, and after 10 days, the cold symptoms are cleared.

Case 2: liu Mou male, 69 years old, senile cataract (both eyes), nasopharyngeal malignant tumor, arterial stent post-implantation state. Acute nasopharyngitis (common cold), sore throat, dry mouth, nasal obstruction, and tired body. The prescription prescribed by doctors contains cold medicines, wherein the cold medicines are the medicine composition in the embodiment, 20mL of the medicine composition is taken three times a day each time, and after 3 days of taking, the symptoms of pharyngalgia are relieved, and after two weeks of taking, the symptoms of pharyngalgia, dry mouth and body fatigue disappear, and the symptoms of nasal obstruction are relieved.

Case 3: hu Mou male, 60 years old, tympanic membrane perforation (left tympanic membrane perforation), viral influenza. Headache, nasal obstruction, fatigue, slight aversion to cold. The prescription prescribed by doctors contains cold medicines, wherein the cold medicines are the medicine composition in the embodiment, 20mL of the medicine composition is taken three times a day each time, and after 2 days of taking, the nasal obstruction symptoms are relieved, and the headache-free feeling and the physical fatigue symptoms are recovered.

Case 4: yang Mou female, 38 years old, gestation time of 32 weeks zero 2 days, iron deficiency, pharyngitis, acute upper respiratory infection, bronchitis (common cold). Itching of the throat, cough and nasal obstruction. The prescription prescribed by doctors contains cold medicines, wherein the cold medicines are the medicine composition in the embodiment, and are taken for 20mL each time, three times a day, and after taking for 4 days, symptoms of throat itching and nasal obstruction disappear, and cough symptoms are relieved.

Case 5: liu Mou it is suitable for women, and is suitable for patients with 1 year old for 9 months and 25 days, acute bronchitis, dyspepsia, hypocalcemia, acute upper respiratory infection, acute pharyngitis (common cold), cough for 3 days, fever, and nasal discharge. The prescription prescribed by doctors contains cold medicines, wherein the cold medicines are the medicine composition in the embodiment, 20mL of the medicine composition is taken every time, the medicine composition is taken three times a day, the medicine composition is taken for 2 days and is burned back, and the cold symptoms are cured after 10 days.

The foregoing disclosure is illustrative of the present invention and is not to be construed as limiting the scope of the invention, which is defined by the appended claims.

Claims

1. The pharmaceutical composition for treating the wind-heat type common cold is characterized by being prepared from the following components in parts by mass: 5-15 parts of Japanese dock root bark, 20-30 parts of trigeminal bitter, 20-30 parts of roughhaired holly root, 8-12 parts of hickory nut and 5-9 parts of cordate houttuynia.

2. The pharmaceutical composition for treating wind-heat type common cold according to claim 1, wherein the composition is prepared from the following components in parts by mass: 10 parts of Japanese dock root bark, 25 parts of trigeminal bitter, 25 parts of roughhaired holly root, 10 parts of Indian screwtree root and 7 parts of heartleaf houttuynia herb.

3. A method of preparing a pharmaceutical composition for the treatment of wind-heat type common cold according to claim 1 or 2, comprising the steps of:

(2) And (3) weighing the materials in the step (1), mixing, adding water, heating and extracting.

4. The method for preparing a pharmaceutical composition for treating wind-heat type common cold according to claim 3, wherein the step (2) comprises filtering the obtained extract, concentrating, standing, and filtering out the precipitate.

5. The method for preparing a pharmaceutical composition for treating wind-heat type common cold according to claim 4, wherein the step (2) comprises concentrating the filtrate by using a double effect concentrator, wherein the temperature of the first effect concentration is 73-83 ℃ and the temperature of the second effect concentration is 58-68 ℃.

6. The method for preparing a pharmaceutical composition for treating wind-heat type common cold according to claim 4, wherein the step (2) comprises adding a proper amount of pure water into the obtained filtrate, adding 300g of sucrose, 25mL of licorice fluid extract, 0.3g of menthol, 0.5g of benzoic acid and 0.5g of ethylparaben into each 1000mL of medicinal liquid, and then boiling and stirring the medicinal liquid uniformly.

7. The method for preparing a pharmaceutical composition for treating wind-heat type common cold according to claim 6, wherein the step (2) comprises detecting a liquid medicine intermediate, wherein the detection index comprises a relative density, and the relative density value is 1.04-1.1.

8. The method for preparing a pharmaceutical composition for treating wind-heat type common cold according to claim 3, wherein the weight of the water added during the extraction in the step (2) is 4-6 times of the weight of the weighed medicine.

9. The method for preparing a pharmaceutical composition for treating wind-heat type common cold according to claim 3, wherein the heating extraction in the step (2) is decoction extraction or reflux extraction.

10. The method for preparing a pharmaceutical composition for treating wind-heat type common cold according to claim 3, wherein the step (2) comprises soaking the medicinal material in water before the heating and extraction.