CN105816548A - Traditional Chinese medicinal composition for treating wind-heat type common colds, and preparation method thereof - Google Patents
Traditional Chinese medicinal composition for treating wind-heat type common colds, and preparation method thereof Download PDFInfo
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- CN105816548A CN105816548A CN201610121853.XA CN201610121853A CN105816548A CN 105816548 A CN105816548 A CN 105816548A CN 201610121853 A CN201610121853 A CN 201610121853A CN 105816548 A CN105816548 A CN 105816548A
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Abstract
The invention relates to a traditional Chinese medicinal composition for treating wind-heat type common colds. A preparation method of the traditional Chinese medicinal composition comprises the following steps: respectively adding water to Elephantopus scaber L., Herba Lysimachiae, Microcos paniculata Linn, licorice root, holly root, Helicteres angustifolia and licorice root to extract, and adsorbing obtained extracts with AB-8 type macroporous resin; respectively adding ethanol to Herb of Triquetrous Tadehagi and Herba Lysimachiae to extract, and adsorbing obtained extracts with D101 macroporous resin; adding water to Dicliptera chinensis, Merremia hederacea and Mussaenda pubescens to extract; and adding ethanol to Ammophila Arenaria, Centella asiatica, Elephantopus scaber L. and Microcos paniculata Linn to extract, carrying out ultramicro crushing on holly root to form finest powder, and mixing and drying the finest powder with all above extracts to obtain the traditional Chinese medicinal composition. Animal pharmacodynamic experiment and clinic test results prove that the traditional Chinese medicinal composition has better fever relieving, pain easing, inflammation prevention and throat disinhibiting effects than traditional Chinese medicinal compositions in the prior art.
Description
Technical field
The present invention relates to the Chinese medicine composition for the treatment of flu, be specifically related to a kind of Chinese medicine composition treating anemopyretic cold and preparation method thereof.
Background technology
Flu is common clinical, frequently-occurring disease, and 90% caused by virus infects.The traditional Chinese medical science is thought, be mainly affected by the cold, wind heat or time row epidemic disease poison caused by, thus flu is divided into anemofrigid cold, anemopyretic cold, summer-heat damp cold and influenza four class, the symptom of each of which is different, discusses according to modern doctor, and anemopyretic cold is mainly heresy criminal's table of wind heat, lung qi is become estranged caused, mainly show as aversion to cold, heating, head-body-pain, parched throat or pharyngalgia, the symptoms such as nasal obstruction, flows turbid nasal discharge, floating and rapid pulse.Application tcm treatment according to syndrome differentiation, it is possible to treating both the principal and secondary aspects of a disease, than Western medicine, original in style.Anemopyretic cold is preferably controlled with wind-dispelling heat-dissipating, and heat-clearing and toxic substances removing is main, uses Method of Relieving Exterior Syndrome with Pungent-Cool Drugs to give suitable treatment.
Proving according to modern study, many relieving the exterior syndrome heat-clearing and detoxifying herbs also have certain antiviral and improve the effect of body immunity.The feature of Western medicine coldrex is that specific aim is relatively strong, effect is very fast in terms of relief of symptoms, and shortcoming is big multipair viral without therapeutical effect and antiinflammation, i.e. cure the symptoms, not the disease in institute Weihe.Compared with Western medicine, dialectical ask originally be treatment by Chinese herbs flu advantage, it can shorten the course of disease, also have positive role in terms of preventing cold.
In 12 taste medical materials, the character of active component is different, it has been disclosed that compound recipe Herba Lysimachiae heat-clearing granule single due to extracting method, cause substantial amounts of active component cannot extract completely, or extraction ratio be low, affects the performance of drug effect.
Accordingly, it is desirable to provide a kind of active component extraction ratio that can improve medicine, to improve the preparation method of pharmaceutical effectiveness.
Summary of the invention
It is an object of the invention to provide the Chinese medicine composition of the treatment anemopyretic cold of a kind of determined curative effect.
A kind of Chinese medicine composition treating anemopyretic cold that the present invention provides, the preparation method of its active component comprises the following steps:
(1) following weight portion medical material is weighed: Radix Helicteris 350-400 part, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 350-400 part, Herba Kalimeridis grass 350-400 part, Herba Centellae 350-400 part, Herba Diclipterae Chinensis 230-300 part, hedge hurdle net 230-300 part, Radix Mussaendae 230-300 part, Elephantopus scaber L. 230-300 part, Herba Lysimachiae 230-300 part, Folium Microcoris paniculatae 230-300 part, Radix Glycyrrhizae 230-300 part, Flos Ilicis Asprellae 500-700 part；
(2) Radix Helicteris, Radix Glycyrrhizae are taken, respectively after water soaking, heating extraction, to filter, filtrate concentrates, and obtains Radix Helicteris, liquorice beverage extracting solution；Respectively with after AB-8 type absorption with macroporous adsorbent resin, first it is washed with deionized water post, then is 20%, 40%, 60%, 80% ethanol by percent by volume successively and dehydrated alcohol carries out eluting, discard water elution part, collecting ethanol elution part, merge, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution；
(3) Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae are taken, adding percent by volume respectively is that 50-70% soak with ethanol post-heating extracts, and filters, filtrate recycling ethanol, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, use D101 macroporous resin adsorption respectively, be first washed with deionized water post, then 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol, dehydrated alcohol carry out eluting successively, discard water elution part, collecting ethanol elution part, merge, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution；
(4) take Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after soaking, supersound extraction, filter, merging filtrate, concentrate, obtain water extracting liquid；
(5) take Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 50-70% soak with ethanol, supersound extraction, filter, merging filtrate, reclaim ethanol, obtain alcohol extraction concentrated solution；
(6) take Flos Ilicis Asprellae, be ground into fine powder with super micron mill；With step 2), 3) 4,5) gained concentrated solution, mix homogeneously, to obtain final product.
Preferably, step 2) described Radix Helicteris, Radix Glycyrrhizae concentrated solution preparation process be as follows:
Take Radix Helicteris, Radix Glycyrrhizae, it is separately added into medical material weight portion 8～20 times of purified water, after soaking 0.5～1h, heating extraction 2～3 times, extraction time 1～3h, filter, it is 1～2g/ml that filtrate is concentrated into medicinal liquid crude drug concentration, obtains Radix Helicteris, liquorice beverage extraction concentrated solution, respectively with AB-8 type absorption with macroporous adsorbent resin, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2～4BV/h；First with 2～4BV deionization washings, eluting is carried out the most successively with 3～5BV 20% ethanol, 3～5BV 40% ethanol, 3～5BV 60% ethanol, 2～4BV 80% ethanol, 2～4BV dehydrated alcohol, elution speed is 2～3BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution.
Preferably, step 2) described Radix Helicteris, Radix Glycyrrhizae concentrated solution preparation process be as follows:
Take Radix Helicteris, Radix Glycyrrhizae, it is separately added into 12 times of purified water of medical material weight portion, after soaking 0.5h, heating extraction 2～3 times, extraction time 1～1.5h, filter, it is 1.5g/ml that filtrate is concentrated into crude drug concentration, obtains Radix Helicteris, liquorice beverage extraction concentrated solution, respectively with AB-8 type absorption with macroporous adsorbent resin, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2BV/h；First wash with 2BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 2BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution.
Preferably, step 3) described Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution preparation process be as follows:
Take Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae, it is separately added into the 50-70% ethanol of medical material weight portion 10～20 times, after soaking 30～60min, temperature 60-80 DEG C, heating extraction 2～3 times, time is 1.5～3h, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 1～2mg/mL, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, using D101 macroporous resin adsorption respectively, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2～3BV/h；First with 2～4BV deionization washings, eluting is carried out the most successively with 3～5BV 20% ethanol, 3～5BV 40% ethanol, 3～5BV 60% ethanol, 2～4BV 80% ethanol, 2～4BV dehydrated alcohol, elution speed is 3～4BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution.
Preferably, step 3) described Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution preparation process be as follows:
Take Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae, it is separately added into 60% ethanol of medical material weight portion 12 times, after soaking 40min, heating extraction 2 times, extraction time is 1.5～2h, temperature 60 C, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 1～2mg/mL, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, using D101 macroporous resin adsorption respectively, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2BV/h；First wash with 3BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 3BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution.
Preferably, step 4) described Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae concentrated solution preparation process be as follows:
Taking Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after adding medical material weight portion 8～20 times of purified water immersions 1～2h, supersound extraction 2～3 times, ultrasonic power is 150-200W, the time 30～90min, temperature 80-100 DEG C, filters, merging filtrate, concentrates, obtains water extracting liquid.
Preferably, step 4) described Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae concentrated solution preparation process be as follows:
Taking Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after adding 15 times of purified water immersion 1h of medical material weight portion, supersound extraction 3 times, ultrasonic power is 150W, time 60min, temperature 90 DEG C, filters, merging filtrate, concentrates, obtains water extracting liquid.
Preferably, step 5) described Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae concentrated solution preparation process be as follows:
Take Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add medical material weight portion 10～20 times of 50-70% ethanol, after soaking 30～60min, supersound extraction 2～3 times, ultrasonic power is 150-200W, the time 30～60min, temperature 60-80 DEG C, filters, merging filtrate, reclaims ethanol, obtains alcohol extraction concentrated solution.
Preferably, step 5) described Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae concentrated solution preparation process be as follows:
Taking Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 12 times of 60% ethanol of medical material weight portion, after soaking 40min, supersound extraction 2 times, ultrasonic power is 150W, time 45min, temperature 60 C, filters, merging filtrate, reclaims ethanol, obtains alcohol extraction concentrated solution.
Preferably, the preparation method of its active component of this Chinese medicine composition active component comprises the following steps:
(1) following weight portion medical material is weighed: Radix Helicteris 384 parts, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 384 parts, Herba Kalimeridis grass 384 parts, Herba Centellae 384 parts, Herba Diclipterae Chinensis 256 parts, 256 parts of hedge hurdle net, Radix Mussaendae 256 parts, Elephantopus scaber L. 256 parts, Herba Lysimachiae 256 parts, Folium Microcoris paniculatae 256 parts, 256 parts of Radix Glycyrrhizae, Flos Ilicis Asprellae 572 parts；
(2) Radix Helicteris, Radix Glycyrrhizae are taken, it is separately added into 12 times of purified water of medical material weight portion, after soaking 0.5h, heating, extract 3 times, extraction time is 1～1.5h, filters, and it is 1.5g/ml that filtrate is concentrated into crude drug concentration, obtain Radix Helicteris, liquorice beverage extracts concentrated solution, respectively with AB-8 type absorption with macroporous adsorbent resin, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2BV/h；First wash with 2BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 2BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution；
(3) Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae are taken, it is separately added into 60% ethanol of medical material weight portion 12 times, after soaking 40min, heating extraction 2 times, time is 1.5～2h, temperature 60 C, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 1～2mg/mL, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, using D101 macroporous resin adsorption respectively, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2BV/h；First wash with 3BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 3BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution；
(4) taking Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after adding 15 times of purified water immersion 1h of medical material weight portion, supersound extraction 3 times, ultrasonic power is 150W, time 60min, temperature 90 DEG C, filters, merging filtrate, concentrates, obtains water extracting liquid；
(5) take Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 12 times of 60% ethanol of medical material weight portion, after soaking 40min, supersound extraction 2 times, ultrasonic power is 150W, time 45min, temperature 60 C, filters, merging filtrate, reclaims ethanol, obtains alcohol extraction concentrated solution；
(6) take Flos Ilicis Asprellae, be ground into fine powder with super micron mill；With step 2), 3) 4,5) gained concentrated solution mixs homogeneously, to obtain final product.
Present invention also offers the preparation containing above-mentioned Chinese medicine composition, said preparation is separately made by Chinese medicine composition or is made up of Chinese medicine composition and pharmaceutically acceptable carrier.
Described preparation is solid preparation or liquid preparation, and solid preparation is tablet, capsule or granule；Liquid preparation is mixture or oral liquid.
Described pharmaceutically acceptable carrier is in lactose, starch, dextrin, mannitol, sucrose, hydroxypropyl methylcellulose, cross-linking sodium carboxymethyl cellulose, low-substituted hydroxypropyl cellulose, microcrystalline Cellulose, micropowder silica gel, pregelatinized Starch, sorbitol, magnesium stearate, Pulvis Talci, hydroxypropyl methylcellulose, low-substituted hydroxypropyl cellulose, sodium benzoate, potassium sorbate, sucrose or aspartame, monosodium glutamate, Mel, orange flavor one or more.
The preparation method of Chinese medicinal composition preparation of the present invention, the method can use conventional formulation technique to be prepared as various common dosage forms.
Such as:
Method 1: Chinese medicinal composition capsules agent of the present invention
Take Chinese medicine composition preparation process 6) in gained Chinese medicine composition, be dried to dried cream powder, add capsule and commonly use adjuvant, production method is prepared into the capsule of Chinese medicine composition of the present invention routinely.
Method 2: Chinese medicine composition tablet of the present invention
Take Chinese medicine composition preparation process 6) in gained Chinese medicine composition, be dried to dried cream powder, add tablet and commonly use adjuvant, production method is prepared into the tablet of Chinese medicine composition of the present invention routinely.
Method 3: Chinese medicinal composition granules of the present invention
Take Chinese medicine composition preparation process 6) in gained Chinese medicine composition, be dried to dried cream powder, add granule and commonly use adjuvant, production method is prepared into the granule of Chinese medicine composition of the present invention routinely.
Method 4: Chinese medicine composition mixture of the present invention
Take Chinese medicine composition preparation process 6) in gained Chinese medicine composition, add mixture commonly use adjuvant, production method is prepared into the mixture of Chinese medicine composition of the present invention routinely.
Method 5: Chinese medicine composition oral liquid of the present invention
Take Chinese medicine composition preparation process 6) in gained Chinese medicine composition, add oral liquid commonly use adjuvant, production method is prepared into the oral liquid of Chinese medicine composition of the present invention routinely.
Method 6: Chinese medicine composition syrup of the present invention
Take Chinese medicine composition preparation process 6) in gained Chinese medicine composition, add syrup commonly use adjuvant, production method is prepared into the syrup of Chinese medicine composition of the present invention routinely.
Present invention also offers above-mentioned Chinese medicine composition or preparation and improve the application of therapeutic effect when preparation treatment anemopyretic cold disease.
Chinese medicine composition effective ingredient disclosed in this invention is to be prepared from by raw materials such as Radix Helicteris, Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Kalimeridis grass, Herba Centellae, Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, Elephantopus scaber L., Herba Lysimachiae, Folium Microcoris paniculatae, Radix Glycyrrhizae, Flos Ilicis Asprellae, Radix Helicteris, Radix Glycyrrhizaes.Inventor finds during preparation research for many years, identical drug dose, different extraction processes prepare the active component of gained, also bigger difference and emphasis can be there is in its pharmacological action, for this, applicant combines pharmacological evaluation to the Chinese medicine composition of the present invention and has carried out a series of technical study, sum up the process for extracting of the present invention, and experiments verify that such improvement brings drug effect potentiation: when the Chinese medicine composition of extraction purification gained is used for treating anemopyretic cold, it is antipyretic, analgesia, antiinflammatory, sore-throat relieving effect is far superior to the existing extractive technique of identical prescription, clinical efficacy shows, the extract of the present invention is compared with prior art, analgesia, antiinflammatory, there were significant differences for sore-throat relieving curative effect, there is significance progress.
Owing to every taste Chinese crude drug all contains many active component, the most not yet there is any a herb purely and simply to be analyzed and understand all the components, different Chinese medicine combines when extracting with different extracting modes, due to influencing each other and acting between solvent, temperature and heterogeneity, extremely complex chemical reaction can be produced.It is the most unilateral for by prior art, certain taste medical material only being detected wherein one, two taste effective ingredient, and the change indicator of part effective ingredient cannot really judge the curative effect change of compound Chinese patent medicine.Therefore, contrast different extracting mode quality in terms of certain therapeutic use by effect experiment at present, be to best embody out the method that whether reasonable extracting mode is.
We contrast antipyretic, the analgesia of different extracting modes, antiinflammatory, sore-throat relieving curative effect by rat paw edema, mice acetic acid induced pain and mice granuloma induced by implantation of cotton pellets zoopery.
Inventor has carried out a large amount of systematic research experiment to the extracting method of each medical material of the present invention with the excellent effect of medicine, by medical material is split as many groups, extract by different methods, finally obtain the extractive technique scheme of the present invention, make the medical material of same recipe, given play to therapeutic purposes more clearly, the effect of more remarkable treatment effect.
A kind of Chinese medicine composition treating anemopyretic cold that the present invention provides has the advantage that
1, Chinese medicine composition of the present invention is antipyretic, analgesia, antiinflammatory, sore-throat relieving effect are obvious, compare with matched group, each administration group can reduce rat paw edema caused by Carrageenan, the writhing incubation period extending acetic acid induced mice and reduce the writhing number of times of mice in 15min, reduce the weight of mice granuloma induced by implantation of cotton pellets, be respectively provided with obvious inhibiting effect.
2, the present invention compares with comparative example, there is pole significant difference (P ＜ 0.01) or significant difference (P ＜ 0.05).Test of pesticide effectiveness result shows, the present invention provide Chinese medicine composition is antipyretic, analgesia, antiinflammatory, sore-throat relieving effect are better than prior art.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
If no special instructions, the concentration of alcohol in the present invention is percent by volume.
Embodiment 1:
(1) following weight portion medical material is weighed: Radix Helicteris 384g, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 384g, Herba Kalimeridis grass 384g, Herba Centellae 384g, Herba Diclipterae Chinensis 256g, hedge hurdle net 256g, Radix Mussaendae 256g, Elephantopus scaber L. 256g, Herba Lysimachiae 256g, Folium Microcoris paniculatae 256g, Radix Glycyrrhizae 256g, Flos Ilicis Asprellae 572g；
(2) Radix Helicteris, Radix Glycyrrhizae are taken, it is separately added into 12 times of purified water of medical material weight portion, after soaking 0.5h, heating, extract 3 times, extraction time 1.5h, 1h, 1h respectively, filter, and it is 1.5g/ml that filtrate is concentrated into crude drug concentration, obtain Radix Helicteris, liquorice beverage extracts concentrated solution, respectively with AB-8 type absorption with macroporous adsorbent resin, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2BV/h；First wash with 2BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 2BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution；
(3) Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae are taken, it is separately added into 60% ethanol of medical material weight portion 12 times, after soaking 40min, heating extraction 2 times, time is respectively 2h, 1.5h, temperature 60 C, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 2mg/mL, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, using D101 macroporous resin adsorption respectively, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2BV/h；First wash with 3BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 3BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution；
(4) taking Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after adding 15 times of purified water immersion 1h of medical material weight portion, supersound extraction 3 times, time 60min, ultrasonic power is 150W, temperature 90 DEG C, filters, merging filtrate, concentrates, obtains water extracting liquid；
(5) take Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 12 times of 60% ethanol of medical material weight portion, after soaking 40min, supersound extraction 2 times, ultrasonic power is 150W, time 45min, temperature 60 C, filters, merging filtrate, reclaims ethanol, obtains alcohol extraction concentrated solution；
(6) take Flos Ilicis Asprellae, be ground into fine powder with super micron mill；With step 2), 3) 4,5) gained concentrated solution mixs homogeneously, to obtain final product.
Embodiment 2:
(1) following weight portion medical material is weighed: Radix Helicteris 350g, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 350g, Herba Kalimeridis grass 350g, Herba Centellae 350g, Herba Diclipterae Chinensis 230g, hedge hurdle net 230g, Radix Mussaendae 230g, Elephantopus scaber L. 230g, Herba Lysimachiae 230g, Folium Microcoris paniculatae 230g, Radix Glycyrrhizae 230g, Flos Ilicis Asprellae 500g；
(2) Radix Helicteris, Radix Glycyrrhizae are taken, it is separately added into 8 times of purified water of medical material weight portion, after soaking 1h, heating, extract 3 times, extraction time 3h, 2h, 1h respectively, filter, and it is 1g/ml that filtrate is concentrated into crude drug concentration, obtain Radix Helicteris, liquorice beverage extracts concentrated solution, respectively with AB-8 type absorption with macroporous adsorbent resin, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2BV/h；First wash with 2BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 2BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution；
(3) Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae are taken, it is separately added into 50% ethanol of medical material weight portion 10 times, after soaking 30min, heating extraction 2 times, time is respectively 3h, 1.5h, temperature 60 C, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 1mg/mL, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, using D101 macroporous resin adsorption respectively, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2BV/h；First wash with 3BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 3BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of the ethanol elution variable concentrations of variable concentrations, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution；
(4) taking Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after adding 8 times of purified water immersion 1h of medical material weight portion, supersound extraction 3 times, each time 30min, ultrasonic power is 150W, temperature 90 DEG C, filters, merging filtrate, concentrates, obtains water extracting liquid；
(5) take Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 10 times of 50% ethanol of medical material weight portion, after soaking 30min, supersound extraction 2 times, ultrasonic power is 150W, and each time is 30min, temperature 80 DEG C, filters, merging filtrate, reclaims ethanol, obtains alcohol extraction concentrated solution；
(6) take Flos Ilicis Asprellae, be ground into fine powder with super micron mill；Combining step 2), 3) 4,5) gained concentrated solution, add Flos Ilicis Asprellae fine powder mix homogeneously, to obtain final product.
Embodiment 3:
(1) following weight portion medical material is weighed: Radix Helicteris 350g, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 350g, Herba Kalimeridis grass 350g, Herba Centellae 350g, Herba Diclipterae Chinensis 230g, hedge hurdle net 230g, Radix Mussaendae 230g, Elephantopus scaber L. 230g, Herba Lysimachiae 230g, Folium Microcoris paniculatae 230g, Radix Glycyrrhizae 230g, Flos Ilicis Asprellae 500g；
(2) Radix Helicteris, Radix Glycyrrhizae are taken, it is separately added into 20 times of purified water of medical material weight portion, after soaking 1h, heating, extract 3 times, extraction time 3h, 2h, 1h respectively, filter, and it is 2g/ml that filtrate is concentrated into crude drug concentration, obtain Radix Helicteris, liquorice beverage extracts concentrated solution, respectively with AB-8 type absorption with macroporous adsorbent resin, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 4BV/h；First wash with 4BV deionization, eluting is carried out the most successively with 5BV 20% ethanol, 5BV 40% ethanol, 5BV 60% ethanol, 4BV 80% ethanol, 4BV dehydrated alcohol, elution speed is 3BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution；
(3) Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae are taken, it is separately added into 70% ethanol of medical material weight portion 20 times, after soaking 60min, heating extraction 3 times, time is respectively 3h, 2h, 1.5h, temperature 80 DEG C, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 2mg/mL, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, using D101 macroporous resin adsorption respectively, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 3BV/h；First wash with 4BV deionization, eluting is carried out the most successively with 5BV 20% ethanol, 5BV 40% ethanol, 5BV 60% ethanol, 4BV 80% ethanol, 4BV dehydrated alcohol, elution speed is 4BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of the ethanol elution variable concentrations of variable concentrations, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution；
(4) taking Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after adding 20 times of purified water immersion 2h of medical material weight portion, supersound extraction 2 times, each time 30min, ultrasonic power is 150W, temperature 100 DEG C, filters, merging filtrate, concentrates, obtains water extracting liquid；
(5) take Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 10 times of 50% ethanol of medical material weight portion, after soaking 30min, supersound extraction 2 times, ultrasonic power is 150W, and each time is 30min, temperature 60 C, filters, merging filtrate, reclaims ethanol, obtains alcohol extraction concentrated solution；
(6) take Flos Ilicis Asprellae, be ground into fine powder with super micron mill；With step 2), 3) 4,5) gained concentrated solution mixs homogeneously, to obtain final product.
Comparative example 1:
With reference to disclosed compound recipe Herba Lysimachiae heat-clearing granule methods of extraction and preparation, specific as follows:
(1) Radix Helicteris 384g, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 384g, Herba Kalimeridis grass 384g, Herba Centellae 384g, Herba Diclipterae Chinensis 256g, hedge hurdle net 256g, Radix Mussaendae 256g, Elephantopus scaber L. 256g, Herba Lysimachiae 256g, Folium Microcoris paniculatae 256g, Radix Glycyrrhizae 256g, Flos Ilicis Asprellae 572g are weighed.
(2) ten purified water adding 12 times simply such as Flos Ilicis Asprellae is ground into fine powder, remaining Radix Helicteris decoct 2 times, and each 2 hours, decocting liquid filtered, filtrate merges, and being concentrated into relative density is 1.17-1.20 (80 DEG C), mixes with above-mentioned fine powder and appropriate dextrin, granule processed, is dried, to obtain final product.
Comparative example 2: Quan Shuiti
(1) following weight portion medical material is weighed: Radix Helicteris 350g, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 350g, Herba Kalimeridis grass 350g, Herba Centellae 350g, Herba Diclipterae Chinensis 230g, hedge hurdle net 230g, Radix Mussaendae 230g, Elephantopus scaber L. 230g, Herba Lysimachiae 230g, Folium Microcoris paniculatae 230g, Radix Glycyrrhizae 230g, Flos Ilicis Asprellae 500g；
(2) add the purified water of medical material gross weight 12 times, after soaking 0.5h, heating, extract 3 times, extraction time 1.5h, 1h, 1h respectively, to filter, it is 3.6g/ml that filtrate is concentrated into crude drug concentration, obtains water extracting liquid.
Comparative example 3: full alcohol extraction
(1) following weight portion medical material is weighed: Radix Helicteris 350g, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 350g, Herba Kalimeridis grass 350g, Herba Centellae 350g, Herba Diclipterae Chinensis 230g, hedge hurdle net 230g, Radix Mussaendae 230g, Elephantopus scaber L. 230g, Herba Lysimachiae 230g, Folium Microcoris paniculatae 230g, Radix Glycyrrhizae 230g, Flos Ilicis Asprellae 500g；
(2) adding 60% ethanol of medical material gross weight 12 times, after soaking 40min, heating extraction 2 times, the time is respectively 2h, 1.5h, temperature 60 C, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 3.6g/mL.
Pharmacodynamic experiment
1 experiment material
1.1 animal
Kunming mouse, SPF level, male and female half and half, body weight 18～22g, 60；Wistar rat, SPF level, male, body weight 180～220g, 60, provided by Guangxi Medical University's Experimental Animal Center.
1.2 given the test agent and reagent
Embodiment 1, embodiment 2, embodiment 3；The sample of comparative example 1, comparative example 2, comparative example 3 preparation；Acetic acid；Carrageenin；Rotundine Tablets.
1.3 experimental apparatus
Electronic balance；Slide gauge；GF-D800 semi-automatic biochemical analyzer；Hot plate induced pain device；LRH-250A biochemical cultivation case；Biological microscope；96 hole Microtitration plates；CO2Incubator；Micro sample adding appliance；Inverted microscope.
2 experimental techniques
The impact of rat paw edema caused by 2.1 Carrageenan
Take SPF level Wistar rat 80, male, body weight 180～220g, it is randomly divided into 8 groups, often group 10.Three groups give embodiment 1, embodiment 2, embodiment 3 respectively, and three groups give comparative example 1, comparative example 2, comparative example 3 respectively, and positive controls gives prednisone, blank group gives equal-volume distilled water, it is administered volume and is 10ml/kg, be given daily 1 time, continuous 7 days.Last i.e. measures the thickness at each Mus right metapedes sole of the foot labelling position as normal value using slide gauge after being administered, and it is subcutaneous so that scorching to inject each Mus right metapedes sole of the foot with 1% carrageenin 50 μ l.After Yu Zhiyan 1,3,6h be measured in the same method the thickness at each Mus right metapedes sole of the foot labelling position respectively as causing value after inflammation, so that value and the difference of normal value are as swelling value after cause inflammation after inflammation.
2.2 impacts on mice acetic acid induced pain
Take SPF level Kunming mouse 80, male and female half and half, body weight 18～22g, be randomly divided into 8 groups, often group 10.The sample that gavage gives embodiment 1-3 and prepared by comparative example 1-3 respectively, positive controls gives rotundine, and blank group gives the distilled water of same volume.Being administered after 1h, every mouse peritoneal injects 0.7% glacial acetic acid, 0.1ml/10g, the writhing incubation period of mice and the writhing response number of times of mice in 15min after record injection.
2.3 impacts on mouse hot-plate induced pain
Take SPF level Kunming mouse some, female, 18～22g, test first 1 day and measure pain threshold with the hot plate induced pain device that baseplate temp is 55 ± 0.5 DEG C, choose pain threshold mice in the range of 5～60s 80.Test same day, then it is measured in the same method pain threshold based on pain threshold 1 time, it is divided into 8 groups by pain threshold, often group 10.The sample that three groups give embodiment 1-3 respectively and prepared by comparative example 1-3, positive controls gives rotundine, and blank group gives equal-volume distilled water, is administered volume and is 20ml/kg.After being administered 0.5,1,1.5,2,3h be measured in the same method the pain threshold of each Mus, after medicine, value deducts the difference of basic value and rises in value as the threshold of pain after medicine.
2.4 mice cotton balls implants experiment
Experiment terminates first 8 days, the hair at the axillary fossa of mice both sides is sloughed with depilator, mice makees otch under ether light anaesthesia aseptic condition, by cotton balls of having weighed, through autoclaving, each cotton balls plus ampicillin 1mg/0.1mL again, after 50 DEG C of oven for drying, implant mice both sides axillary fossa subcutaneous, blank group it is randomly divided into after bundle, positive group and 3 embodiments and 3 comparative example test medicine groups, blank group gives distilled water, positive drug group gives aspirin, the sample that therapeutic component does not give embodiment 1-3 and prepared by comparative example 1-3, every day gavage 1 time, gavage 30d continuously.Experiment terminates the same day again to a given the test agent, and after 1 hour, disconnected neck puts to death mice, peels off and take out cotton balls granulation tissue, weighs, deduct raw cotton ball weight, be granuloma net amount in 60 DEG C of baking ovens after being dried 1 hour.Relatively each dosage group and blank group granuloma weight.
3 statistical procedures
Experimental data uses SPSS11.0 to carry out statistical procedures, continuous data represents with x ± s, compares the t inspection using two sample averages between group, and between many groups, sample average compares employing variance analysis, result, as shown in table 1-4, thinks there is significant difference with P ＜ 0.05.
4 results and analysis
The impact of rat paw edema caused by 4.1 Carrageenan the results are shown in Table 1
The impact of rat paw edema caused by Carrageenan respectively organized by table 1
Note: comparing with blank group, * represents that P ＜ 0.05, * * represent P ＜ 0.01.
Being shown by table 1 result, after embodiment 1-3 group, cause inflammation 1h of comparative example 1-3 group, 3h, 6h, rat paw edema value is all significantly lower than blank group (P < 0.05 or P < 0.01)；And embodiment 1-3 group indices is substantially better than comparative example 1-3 group.
4.2 the results are shown in Table 2 to the impact of mice acetic acid induced pain
The effect that Dichlorodiphenyl Acetate causes mouse writhing to react respectively organized by table 2
Note: comparing with blank group, * represents that P ＜ 0.05, * * represent P ＜ 0.01.
Being shown by table 2 result, embodiment 1-3 group, the writhing of comparative example 1-3 group are all considerably longer than blank group (P < 0.05 or P < 0.01) incubation period；And the 15min writhing number of times of each administration group is all less than blank group (P < 0.05 or P < 0.01), embodiment 1-3 group indices is substantially better than comparative example 1-3 group.
4.3 the results are shown in Table 3 to the impact of mouse hot-plate induced pain
The impact of Dichlorodiphenyl Acetate induced mice peritoneum inflammatory exudation respectively organized by table 3
Note: comparing with blank group, * represents that P ＜ 0.05, * * represent P ＜ 0.01.
Shown by table 3 result, embodiment 1-3 group, comparative example 1-3 group OD value all significantly lower than blank group (P < 0.05 or P < 0.01)；And the suppression ratio of each administration group is all less than blank group (P < 0.05 or P < 0.01), embodiment 1-3 group indices is substantially better than comparative example 1-3 group.
4.4 the results are shown in Table 4 to the impact of mice granuloma induced by implantation of cotton pellets
Table 4 each embodiment group on the impact of mice granuloma induced by implantation of cotton pellets (N=10)
Note: comparing with blank group, Δ represents that P ＜ 0.05, Δ Δ represent P ＜ 0.01；Comparing with model group, * represents that P ＜ 0.05, * * represent P ＜ 0.01.
Being shown by table 4 result, compared with matched group, embodiment 1-3 group, the granuloma net amount of comparative example 1-3 group are all decreased obviously, and have significant difference (P < 0.05 or P < 0.01) through statistical disposition；And each embodiment 1-3 group indices is substantially better than comparative example 1-3 group.
Conclusion: compound recipe money grass particle has good antipyretic-antalgic, antiinflammatory, sore-throat relieving effect at treatment anemopyretic cold.Experimental result shows, each test medicine group has obvious inhibiting effect to rat paw edema caused by Carrageenan, and compared with matched group, the therapeutic effect of embodiment 1-3 group relatively comparative example 1-3 group is the most notable (P < 0.01), shows that the present invention has obvious antiinflammatory action；Each test medicine group all can extend writhing incubation period of acetic acid induced pain mice and reduce 15min in the writhing number of times of mice, have pole significant difference (P < 0.05 or P < 0.01), result shows, the present invention has obvious analgesic activity；Compared with matched group, each test medicine group the most substantially reduces the weight of mice granuloma induced by implantation of cotton pellets, and the therapeutic effect the most notable (P < 0.01) of embodiment 1-3 group relatively comparative example 1-3 group, and result shows, the present invention has obvious sore-throat relieving effect.Embodiment group all has good curative effect with comparative example group at treatment anemopyretic cold, but the therapeutic effect of embodiment group is the most notable, relatively matched group is respectively provided with significant difference (P < 0.01), test result indicate that, medicine of the present invention has the most antipyretic, analgesia, antiinflammatory, sore-throat relieving effect.
Although, use general explanation, detailed description of the invention and test, the present invention is described in detail, but on the basis of the present invention, can make some modifications or improvements it, and this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (10)
1. the Chinese medicine composition treating anemopyretic cold, it is characterised in that the preparation method of its active component comprises the following steps:
(1) following weight portion medical material is weighed: Radix Helicteris 350-400 part, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 350-400 part, Herba Kalimeridis grass 350-400 part, Herba Centellae 350-400 part, Herba Diclipterae Chinensis 230-300 part, hedge hurdle net 230-300 part, Radix Mussaendae 230-300 part, Elephantopus scaber L. 230-300 part, Herba Lysimachiae 230-300 part, Folium Microcoris paniculatae 230-300 part, Radix Glycyrrhizae 230-300 part, Flos Ilicis Asprellae 500-700 part；
(2) Radix Helicteris, Radix Glycyrrhizae are taken, respectively after water soaking, heating extraction, to filter, filtrate concentrates, and obtains Radix Helicteris, liquorice beverage extracting solution；Respectively with after AB-8 type absorption with macroporous adsorbent resin, first it is washed with deionized water post, then is 20%, 40%, 60%, 80% ethanol by percent by volume successively and dehydrated alcohol carries out eluting, discard water elution part, collecting ethanol elution part, merge, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution；
(3) Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae are taken, adding percent by volume respectively is that 50-70% soak with ethanol post-heating extracts, and filters, filtrate recycling ethanol, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, use D101 macroporous resin adsorption respectively, be first washed with deionized water post, then 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol, dehydrated alcohol carry out eluting successively, discard water elution part, collecting ethanol elution part, merge, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution；
(4) take Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after soaking, supersound extraction, filter, merging filtrate, concentrate, obtain water extracting liquid；
(5) take Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 50-70% soak with ethanol, supersound extraction, filter, merging filtrate, reclaim ethanol, obtain alcohol extraction concentrated solution；
(6) take Flos Ilicis Asprellae, be ground into fine powder with super micron mill；With step 2), 3) 4,5) gained concentrated solution, mix homogeneously, to obtain final product.
The Chinese medicine composition for the treatment of anemopyretic cold the most according to claim 1, it is characterised in that step 2) described Radix Helicteris, Radix Glycyrrhizae concentrated solution preparation process be as follows:
Take Radix Helicteris, Radix Glycyrrhizae, it is separately added into 8 ~ 20 times of purified water of medical material weight portion, after soaking 0.5 ~ 1h, heating extraction 2 ~ 3 times, extraction time 1 ~ 3h, filter, it is 1 ~ 2g/ml that filtrate is concentrated into medicinal liquid crude drug concentration, obtains Radix Helicteris, liquorice beverage extraction concentrated solution, respectively with AB-8 type absorption with macroporous adsorbent resin, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2 ~ 4 BV/h；First with 2 ~ 4 BV deionization washings, eluting is carried out the most successively with 3 ~ 5BV 20% ethanol, 3 ~ 5BV 40% ethanol, 3 ~ 5BV 60% ethanol, 2 ~ 4BV 80% ethanol, 2 ~ 4BV dehydrated alcohol, elution speed is 2 ~ 3 BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution.
The Chinese medicine composition for the treatment of anemopyretic cold the most according to claim 2, it is characterised in that step 2) described Radix Helicteris, Radix Glycyrrhizae concentrated solution preparation process be as follows:
Take Radix Helicteris, Radix Glycyrrhizae, it is separately added into 12 times of purified water of medical material weight portion, after soaking 0.5h, heating extraction 2 ~ 3 times, extraction time 1 ~ 1.5h, filter, it is 1.5g/ml that filtrate is concentrated into crude drug concentration, obtains Radix Helicteris, liquorice beverage extraction concentrated solution, respectively with AB-8 type absorption with macroporous adsorbent resin, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2 BV/h；First wash with 2BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 2 BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution.
The Chinese medicine composition for the treatment of anemopyretic cold the most according to claim 1, it is characterised in that Herba Desmodii Triquetri (Herba Tadehagi Triquetri) described in step 3), Herba Lysimachiae concentrated solution preparation process are as follows:
Take Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae, it is separately added into the 50-70% ethanol of medical material weight portion 10 ~ 20 times, after soaking 30 ~ 60min, temperature 60-80 DEG C, heating extraction 2 ~ 3 times, time is 1.5 ~ 3h, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 1 ~ 2mg/mL, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, using D101 macroporous resin adsorption respectively, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2 ~ 3 BV/h；First wash with 2 ~ 4BV deionization, eluting is carried out the most successively with 3 ~ 5BV 20% ethanol, 3 ~ 5BV 40% ethanol, 3 ~ 5BV 60% ethanol, 2 ~ 4BV 80% ethanol, 2 ~ 4BV dehydrated alcohol, elution speed is 3 ~ 4 BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution.
The Chinese medicine composition for the treatment of anemopyretic cold the most according to claim 4, it is characterised in that Herba Desmodii Triquetri (Herba Tadehagi Triquetri) described in step 3), Herba Lysimachiae concentrated solution preparation process are as follows:
Take Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae, it is separately added into 60% ethanol of medical material weight portion 12 times, after soaking 40min, heating extraction 2 times, extraction time is 1.5 ~ 2h, temperature 60 C, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 1 ~ 2mg/mL, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, using D101 macroporous resin adsorption respectively, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2 BV/h；First wash with 3BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 3 BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution.
The Chinese medicine composition for the treatment of anemopyretic cold the most according to claim 1, it is characterised in that Herba Diclipterae Chinensis described in step 4), hedge hurdle net, Radix Mussaendae concentrated solution preparation process are as follows:
Taking Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after adding 8 ~ 20 times of purified water immersion 1 ~ 2h of medical material weight portion, supersound extraction 2 ~ 3 times, ultrasonic power is 150-200W, each time 30 ~ 90min, temperature 80-100 DEG C, filters, merging filtrate, concentrates, obtains water extracting liquid.
The Chinese medicine composition for the treatment of anemopyretic cold the most according to claim 6, it is characterised in that Herba Diclipterae Chinensis described in step 4), hedge hurdle net, Radix Mussaendae concentrated solution preparation process are as follows:
Taking Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after adding 15 times of purified water immersion 1h of medical material weight portion, supersound extraction 3 times, ultrasonic power is 150W, time 60min, temperature 90 DEG C, filters, merging filtrate, concentrates, obtains water extracting liquid.
The Chinese medicine composition for the treatment of anemopyretic cold the most according to claim 1, it is characterised in that Herba Kalimeridis grass described in step 5), Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae concentrated solution preparation process are as follows:
Take Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 10 ~ 20 times of 50-70% ethanol of medical material weight portion, after soaking 30 ~ 60min, supersound extraction 2 ~ 3 times, ultrasonic power is 150-200W, time is 30 ~ 60min every time, temperature 60-80 DEG C, filters, merging filtrate, reclaim ethanol, obtain alcohol extraction concentrated solution.
The Chinese medicine composition for the treatment of anemopyretic cold the most according to claim 8, it is characterised in that Herba Kalimeridis grass described in step 5), Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae concentrated solution preparation process are as follows:
Taking Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 12 times of 60% ethanol of medical material weight portion, after soaking 40min, supersound extraction 2 times, ultrasonic power is 150W, time 45min, temperature 60 C, filters, merging filtrate, reclaims ethanol, obtains alcohol extraction concentrated solution.
The Chinese medicine composition for the treatment of anemopyretic cold the most according to claim 1, it is characterised in that the preparation method of its active component comprises the following steps:
(1) following weight portion medical material is weighed: Radix Helicteris 384 parts, Herba Desmodii Triquetri (Herba Tadehagi Triquetri) 384 parts, Herba Kalimeridis grass 384 parts, Herba Centellae 384 parts, Herba Diclipterae Chinensis 256 parts, 256 parts of hedge hurdle net, Radix Mussaendae 256 parts, Elephantopus scaber L. 256 parts, Herba Lysimachiae 256 parts, Folium Microcoris paniculatae 256 parts, 256 parts of Radix Glycyrrhizae, Flos Ilicis Asprellae 572 parts；
(2) Radix Helicteris, Radix Glycyrrhizae are taken, it is separately added into 12 times of purified water of medical material weight portion, after soaking 0.5h, heating, extract 3 times, extraction time is 1 ~ 1.5h, filters, and it is 1.5g/ml that filtrate is concentrated into crude drug concentration, obtain Radix Helicteris, liquorice beverage extracts concentrated solution, respectively with AB-8 type absorption with macroporous adsorbent resin, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2 BV/h；First wash with 2BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 2 BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Radix Helicteris, Radix Glycyrrhizae concentrated solution；
(3) Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae are taken, it is separately added into 60% ethanol of medical material weight portion 12 times, after soaking 40min, heating extraction 2 times, time is 1.5 ~ 2h, temperature 60 C, filters, and filtrate recycling ethanol to concentration is with crude drug gauge 1 ~ 2mg/mL, obtain Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae extracting solution, using D101 macroporous resin adsorption respectively, medicinal liquid upper column quantity is 5g/g based on crude drug amount/dried resin, and loading speed is 2 BV/h；First wash with 3BV deionization, eluting is carried out the most successively with 3BV 20% ethanol, 3BV 40% ethanol, 3BV 60% ethanol, 2BV 80% ethanol, 2BV dehydrated alcohol, elution speed is 3 BV/h, discard water elution part, collect ethanol elution part, merging the ethanol elution of variable concentrations, concentrating under reduced pressure obtains Herba Desmodii Triquetri (Herba Tadehagi Triquetri), Herba Lysimachiae concentrated solution；
(4) taking Herba Diclipterae Chinensis, hedge hurdle net, Radix Mussaendae, after adding 15 times of purified water immersion 1h of medical material weight portion, supersound extraction 3 times, ultrasonic power is 150W, time 60min, temperature 90 DEG C, filters, merging filtrate, concentrates, obtains water extracting liquid；
(5) taking Herba Kalimeridis grass, Herba Centellae, Elephantopus scaber L., Folium Microcoris paniculatae, add 12 times of 60% ethanol of medical material weight portion, after soaking 40min, supersound extraction 2 times, ultrasonic power is 150W, time 45min, temperature 60 C, filters, merging filtrate, reclaims ethanol, obtains alcohol extraction concentrated solution；
(6) take Flos Ilicis Asprellae, be ground into fine powder with super micron mill；With step 2), 3) 4,5) gained concentrated solution mixs homogeneously, to obtain final product.
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Application publication date: 20160803 |