CN116676138A - Oncidium essential oil for cigarettes and preparation method and application thereof - Google Patents
Oncidium essential oil for cigarettes and preparation method and application thereof Download PDFInfo
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- CN116676138A CN116676138A CN202310911864.8A CN202310911864A CN116676138A CN 116676138 A CN116676138 A CN 116676138A CN 202310911864 A CN202310911864 A CN 202310911864A CN 116676138 A CN116676138 A CN 116676138A
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- oncidium
- essential oil
- distillation
- cigarettes
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- 241000190074 Oncidium Species 0.000 title claims abstract description 112
- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 99
- 239000000341 volatile oil Substances 0.000 title claims abstract description 76
- 238000002360 preparation method Methods 0.000 title claims abstract description 41
- 238000000199 molecular distillation Methods 0.000 claims abstract description 42
- 238000001256 steam distillation Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims description 46
- 244000063299 Bacillus subtilis Species 0.000 claims description 33
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 33
- 108090000790 Enzymes Proteins 0.000 claims description 33
- 102000004190 Enzymes Human genes 0.000 claims description 33
- 229940088598 enzyme Drugs 0.000 claims description 33
- 238000004821 distillation Methods 0.000 claims description 31
- 239000000203 mixture Substances 0.000 claims description 24
- 239000000843 powder Substances 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 21
- 238000009833 condensation Methods 0.000 claims description 14
- 230000005494 condensation Effects 0.000 claims description 14
- 101710130006 Beta-glucanase Proteins 0.000 claims description 10
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 10
- 102000004142 Trypsin Human genes 0.000 claims description 10
- 108090000631 Trypsin Proteins 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 229940059442 hemicellulase Drugs 0.000 claims description 10
- 108010002430 hemicellulase Proteins 0.000 claims description 10
- 239000012588 trypsin Substances 0.000 claims description 10
- 239000000287 crude extract Substances 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- -1 pectase Proteins 0.000 claims description 7
- 238000000874 microwave-assisted extraction Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 244000284012 Vetiveria zizanioides Species 0.000 claims description 4
- 235000007769 Vetiveria zizanioides Nutrition 0.000 claims description 4
- 108010059820 Polygalacturonase Proteins 0.000 claims description 3
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000011324 bead Substances 0.000 abstract description 38
- 230000001953 sensory effect Effects 0.000 abstract description 24
- 239000000779 smoke Substances 0.000 abstract description 21
- 239000003205 fragrance Substances 0.000 abstract description 17
- 238000004880 explosion Methods 0.000 abstract description 11
- 230000000391 smoking effect Effects 0.000 abstract description 8
- 241000208125 Nicotiana Species 0.000 abstract description 6
- 235000002637 Nicotiana tabacum Nutrition 0.000 abstract description 6
- 230000007794 irritation Effects 0.000 abstract description 6
- 239000002131 composite material Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 19
- 238000000605 extraction Methods 0.000 description 14
- QUKGYYKBILRGFE-UHFFFAOYSA-N benzyl acetate Chemical compound CC(=O)OCC1=CC=CC=C1 QUKGYYKBILRGFE-UHFFFAOYSA-N 0.000 description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 11
- 239000002360 explosive Substances 0.000 description 10
- 238000002347 injection Methods 0.000 description 10
- 239000007924 injection Substances 0.000 description 10
- 238000013441 quality evaluation Methods 0.000 description 7
- OOCCDEMITAIZTP-QPJJXVBHSA-N (E)-cinnamyl alcohol Chemical compound OC\C=C\C1=CC=CC=C1 OOCCDEMITAIZTP-QPJJXVBHSA-N 0.000 description 6
- WRMNZCZEMHIOCP-UHFFFAOYSA-N 2-phenylethanol Chemical compound OCCC1=CC=CC=C1 WRMNZCZEMHIOCP-UHFFFAOYSA-N 0.000 description 6
- UWKAYLJWKGQEPM-UHFFFAOYSA-N 3,7-dimethylocta-1,6-dien-3-yl acetate Chemical compound CC(C)=CCCC(C)(C=C)OC(C)=O UWKAYLJWKGQEPM-UHFFFAOYSA-N 0.000 description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- 229940007550 benzyl acetate Drugs 0.000 description 6
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 description 6
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 description 6
- VAMXMNNIEUEQDV-UHFFFAOYSA-N methyl anthranilate Chemical compound COC(=O)C1=CC=CC=C1N VAMXMNNIEUEQDV-UHFFFAOYSA-N 0.000 description 6
- KVWWIYGFBYDJQC-UHFFFAOYSA-N methyl dihydrojasmonate Chemical compound CCCCCC1C(CC(=O)OC)CCC1=O KVWWIYGFBYDJQC-UHFFFAOYSA-N 0.000 description 6
- 230000009172 bursting Effects 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 206010013911 Dysgeusia Diseases 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- CBOQJANXLMLOSS-UHFFFAOYSA-N ethyl vanillin Chemical group CCOC1=CC(C=O)=CC=C1O CBOQJANXLMLOSS-UHFFFAOYSA-N 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- 235000011187 glycerol Nutrition 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 description 3
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 description 3
- VPKMGDRERYMTJX-CMDGGOBGSA-N 1-(2,6,6-Trimethyl-2-cyclohexen-1-yl)-1-penten-3-one Chemical compound CCC(=O)\C=C\C1C(C)=CCCC1(C)C VPKMGDRERYMTJX-CMDGGOBGSA-N 0.000 description 3
- YCIXWYOBMVNGTB-UHFFFAOYSA-N 3-methyl-2-pentylcyclopent-2-en-1-one Chemical compound CCCCCC1=C(C)CCC1=O YCIXWYOBMVNGTB-UHFFFAOYSA-N 0.000 description 3
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 description 3
- 239000005770 Eugenol Substances 0.000 description 3
- BJIOGJUNALELMI-ONEGZZNKSA-N Isoeugenol Natural products COC1=CC(\C=C\C)=CC=C1O BJIOGJUNALELMI-ONEGZZNKSA-N 0.000 description 3
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 description 3
- OOCCDEMITAIZTP-UHFFFAOYSA-N allylic benzylic alcohol Natural products OCC=CC1=CC=CC=C1 OOCCDEMITAIZTP-UHFFFAOYSA-N 0.000 description 3
- WUOACPNHFRMFPN-UHFFFAOYSA-N alpha-terpineol Chemical compound CC1=CCC(C(C)(C)O)CC1 WUOACPNHFRMFPN-UHFFFAOYSA-N 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- BJIOGJUNALELMI-ARJAWSKDSA-N cis-isoeugenol Chemical compound COC1=CC(\C=C/C)=CC=C1O BJIOGJUNALELMI-ARJAWSKDSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- SQIFACVGCPWBQZ-UHFFFAOYSA-N delta-terpineol Natural products CC(C)(O)C1CCC(=C)CC1 SQIFACVGCPWBQZ-UHFFFAOYSA-N 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- XSNQECSCDATQEL-UHFFFAOYSA-N dihydromyrcenol Chemical compound C=CC(C)CCCC(C)(C)O XSNQECSCDATQEL-UHFFFAOYSA-N 0.000 description 3
- 229930008394 dihydromyrcenol Natural products 0.000 description 3
- 229960002217 eugenol Drugs 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- ZNJFBWYDHIGLCU-HWKXXFMVSA-N jasmonic acid Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-HWKXXFMVSA-N 0.000 description 3
- 229930007744 linalool Natural products 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 229940102398 methyl anthranilate Drugs 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- SATCULPHIDQDRE-UHFFFAOYSA-N piperonal Chemical compound O=CC1=CC=C2OCOC2=C1 SATCULPHIDQDRE-UHFFFAOYSA-N 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 229940116411 terpineol Drugs 0.000 description 3
- BJIOGJUNALELMI-UHFFFAOYSA-N trans-isoeugenol Natural products COC1=CC(C=CC)=CC=C1O BJIOGJUNALELMI-UHFFFAOYSA-N 0.000 description 3
- 235000005744 Bacillus subtilis subsp subtilis Nutrition 0.000 description 2
- 241000948854 Bacillus subtilis subsp. subtilis Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000011218 binary composite Substances 0.000 description 2
- 238000005422 blasting Methods 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- MLUCVPSAIODCQM-NSCUHMNNSA-N crotonaldehyde Chemical compound C\C=C\C=O MLUCVPSAIODCQM-NSCUHMNNSA-N 0.000 description 2
- MLUCVPSAIODCQM-UHFFFAOYSA-N crotonaldehyde Natural products CC=CC=O MLUCVPSAIODCQM-UHFFFAOYSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- RGXWDWUGBIJHDO-UHFFFAOYSA-N ethyl decanoate Chemical compound CCCCCCCCCC(=O)OCC RGXWDWUGBIJHDO-UHFFFAOYSA-N 0.000 description 2
- 229940073505 ethyl vanillin Drugs 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000006187 pill Substances 0.000 description 2
- BBEAQIROQSPTKN-UHFFFAOYSA-N pyrene Chemical compound C1=CC=C2C=CC3=CC=CC4=CC=C1C2=C43 BBEAQIROQSPTKN-UHFFFAOYSA-N 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 239000011206 ternary composite Substances 0.000 description 2
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 2
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 2
- 235000012141 vanillin Nutrition 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- FMMWHPNWAFZXNH-UHFFFAOYSA-N ERM-AC051 Natural products C1=C2C3=CC=CC=C3C=C(C=C3)C2=C2C3=CC=CC2=C1 FMMWHPNWAFZXNH-UHFFFAOYSA-N 0.000 description 1
- 241000218397 Magnolia x soulangeana Species 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 244000290333 Vanilla fragrans Species 0.000 description 1
- 235000009499 Vanilla fragrans Nutrition 0.000 description 1
- 235000012036 Vanilla tahitensis Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthrene Natural products C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 229960005150 glycerol Drugs 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 229960004063 propylene glycol Drugs 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24D—CIGARS; CIGARETTES; TOBACCO SMOKE FILTERS; MOUTHPIECES FOR CIGARS OR CIGARETTES; MANUFACTURE OF TOBACCO SMOKE FILTERS OR MOUTHPIECES
- A24D3/00—Tobacco smoke filters, e.g. filter-tips, filtering inserts; Filters specially adapted for simulated smoking devices; Mouthpieces for cigars or cigarettes
- A24D3/02—Manufacture of tobacco smoke filters
- A24D3/0204—Preliminary operations before the filter rod forming process, e.g. crimping, blooming
- A24D3/0212—Applying additives to filter materials
- A24D3/0216—Applying additives to filter materials the additive being in the form of capsules, beads or the like
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Fats And Perfumes (AREA)
Abstract
The application provides a oncidium essential oil for cigarettes, a preparation method and application thereof, and relates to the technical field of tobacco essence. According to the application, the enzyme-bacterium composite treatment is combined with microwave-assisted steam distillation and molecular distillation for the first time, so that the high-quality oncidium essential oil suitable for cigarettes is obtained. In addition, the oncidium essential oil provided by the application has good stability and can be stored for a long time, and the oncidium essential oil prepared into the explosion beads can be used in cigarettes to effectively improve the fragrance of the cigarettes, reduce the irritation of smoke, enrich and improve the smoking feeling, obviously improve the sensory quality of the cigarettes and reduce the harm of the cigarettes, and is suitable for high-quality cigarettes.
Description
Technical Field
The application relates to the field of tobacco essence, in particular to a oncidium essential oil for cigarettes, and a preparation method and application thereof.
Background
With the increasing attention of people to the relationship between smoking and health, a series of tar reduction measures have been adopted at home and abroad, so that the health risk of consumers can be reduced, but new problems such as light smoke, lack of fragrance and the like are brought.
The aragonite belongs to the multiple stem aerial orchids, has unique flower shape and higher ornamental value, the flower color is pure yellow, magenta and pink, or has brown patterns and spots, and the inflorescence is more varied, and is provided with one or two, ten or hundreds of flowers which are upright. At present, most of researches on the oncidium at home and abroad are the volatile component identification and tissue culture researches. The method has little research on the extraction of the oncidium essence, and no report on the extraction method of the oncidium essential oil suitable for cigarettes exists at present.
Disclosure of Invention
The application aims to provide a oncidium essential oil for cigarettes, and a preparation method and application thereof. The preparation process of the oncidium essential oil provided by the application not only can improve the extraction rate of the oncidium essential oil, but also can effectively reduce the content of harmful substances in smoke, and can obviously reduce the irritation of the smoke and improve the sensory quality and quality of the cigarette when the oncidium essential oil is applied to the cigarette in a bead-bursting mode after proper flavoring.
In one aspect, the application provides a method for preparing a oncidium essential oil, which comprises the following steps:
crushing the oncidium petals to 40-100 meshes to obtain oncidium powder;
step two, mixing the oncidium powder with water, adding an enzyme preparation and bacillus subtilis bacterial liquid, and treating 2-8 h at the temperature of 30-60 ℃ and the pH value of 4-7 to obtain oncidium treatment liquid; the enzyme preparation is selected from one or more of hemicellulase, pectinase, trypsin, acid xylanase and beta-glucanase;
performing microwave-assisted steam distillation on the oncidium treatment liquid, heating to 80-100 ℃ with microwave extraction power of 250W, distilling and extracting for 30-90 min, collecting distillate for separating liquid, and reserving upper-layer fractions to obtain a oncidium crude extract;
and step four, carrying out primary and secondary molecular distillation on the crude extract of the oncidium, and merging the primary molecular distillation light component and the secondary molecular distillation light component to obtain the oncidium essential oil.
Preferably, the microwave-assisted steam distillation is carried out, the temperature is heated to 100 ℃, and the distillation and extraction are carried out for 70 min.
Preferably, the fourth step further comprises a step of inactivation, wherein the inactivation is performed at 100 ℃ for 5 minutes.
Furthermore, the enzyme preparation is hemicellulase, pectase, trypsin, acid xylanase and beta-glucanase with the enzyme activity ratio of (1-9) (2-6) (2-5) (1-5).
Preferably, the enzyme preparation is hemicellulase, pectinase, trypsin, acid xylanase and beta-glucanase with the enzyme activity ratio of 5:3:3:2:4.
More preferably, the enzyme preparation is a food grade liquid enzyme preparation having an enzyme activity of 5-20 mu/g.
Further, the addition amount of the enzyme preparation is 1-1.5% of the mass of the powder of the oncidium, and the addition amount of the bacillus subtilis strain liquid is 0.1-0.5% of the mass of the powder of the oncidium.
Preferably, the addition amount of the enzyme preparation is 1% of the mass of the vetiver powder, and the addition amount of the bacillus subtilis strain liquid is 0.1% of the mass of the vetiver powder.
Further, the bacillus subtilis in the bacillus subtilis liquid is bacillus subtilis CICC24406, and the bacillus subtilis liquid OD 600 The value is 3-4.
Preferably, the bacillus subtilis in the bacillus subtilis liquid is bacillus subtilis CICC24406 and bacillus subtilis liquid OD 600 The value is 3.
The bacillus subtilis is bacillus subtilis [ (]Bacillus subtilis subsp. Subtilis) CICC24406, which is purchased from China center for type culture Collection of microorganisms.
Further, the mass ratio of the oncidium powder to the water is 1 (8-20).
Preferably, the mass ratio of the oncidium powder to the water is 1:10.
Further, the distillation pressure of the primary molecular distillation is 0.1-10 Pa, the sample injection temperature is 60-70 ℃, the distillation temperature is 100-130 ℃, the condensation temperature is 20-40 ℃, the distillation pressure of the secondary molecular distillation is 0.1-10 Pa, the sample injection temperature is 50-70 ℃, the distillation temperature is 150-170 ℃, and the condensation temperature is 60-70 ℃.
Preferably, the distillation pressure of the primary molecular distillation is 5 Pa, the sample introduction temperature is 60 ℃, the distillation temperature is 130 ℃, the condensation temperature is 25 ℃, the distillation pressure of the secondary molecular distillation is 5 Pa, the sample introduction temperature is 50 ℃, the distillation temperature is 160 ℃, and the condensation temperature is 60 ℃.
According to the application, the method of combining the enzyme-bacteria composite preparation with microwave-assisted steam distillation is adopted for the first time to extract the oncidium essential oil, so that the extraction efficiency of the oncidium essential oil is effectively improved, and the problem of overlong microwave-assisted steam distillation extraction time is solved by firstly adopting the enzyme bacteria to treat the oncidium powder. Moreover, the obtained oil has special creamy fragrance, and has good fragrance. The application further adopts molecular distillation to further process and obtain the high-quality oncidium essential oil. The high-quality oncidium essential oil has the characteristics of good stability and rich fragrance, is suitable for the manufacturing process of cigarette bursting beads, and can reduce the content of harmful substances in smoke while improving the smoking experience of a user.
In a preferred embodiment, the preparation method of the Chinese magnolia essential oil comprises the following steps:
crushing the oncidium petals to 40-100 meshes to obtain oncidium powder;
mixing the oncidium powder with water according to a mass ratio of 1 (8-20), adding an enzyme preparation with a mass ratio of 1% -1.5% of the oncidium powder and 0.1% -0.5% of bacillus subtilis liquid, wherein the enzyme preparation comprises hemicellulase, pectase, trypsin, acid xylanase and beta-glucanase with an enzyme activity ratio of (1-9) (2-6) (2-5) (1-5), and treating 2-8 h under the conditions that the temperature is 30-60 ℃ and the pH is 4-7 to obtain the oncidium treatment liquid;
step three, carrying out microwave-assisted steam distillation on the oncidium treatment liquid under normal pressure, heating to 80-100 ℃ with microwave extraction power of 250W, distilling and extracting for 30-90 min, collecting distillate for liquid separation, and reserving upper-layer fractions to obtain a oncidium crude extract;
and fourthly, carrying out primary and secondary molecular distillation on the crude extract of the oncidium, wherein the distillation pressure of the primary molecular distillation is 0.1-10 Pa, the sample injection temperature is 60-70 ℃, the distillation temperature is 100-130 ℃, the condensation temperature is 20-40 ℃, the distillation pressure of the secondary molecular distillation is 0.1-10 Pa, the sample injection temperature is 50-70 ℃, the distillation temperature is 150-170 ℃, the condensation temperature is 60-70 ℃, and the high-quality oncidium essential oil is obtained by inactivating the primary molecular distillation light component and the secondary molecular distillation light component at 100 ℃ for 5 min after the primary molecular distillation light component and the secondary molecular distillation light component are combined.
The preparation method of the bacillus subtilis bacterial liquid comprises the following steps: inoculating Bacillus subtilis CICC24406 into 50 mL LB liquid medium, culturing at 20-40deg.C and 100-200 r/min for 24-72 hr, and OD 600 The value is 3-4.
On the other hand, the application also provides the oncidium essential oil prepared by the method.
In another aspect, the application also provides an essential oil composition, which comprises the oncidium essential oil.
Preferably, the essential oil composition further comprises any one or more of the following components: linalool, dihydromyrcenol, phenethyl alcohol, terpineol, cinnamyl alcohol, benzyl acetate, jasmonate, eugenol, methyl anthranilate, methyl dihydrojasmonate, methyl ionone, isoeugenol, heliotropin, dihydrojasmone, linalool acetate, benzyl acetate, vanillin, ethyl vanillin, propylene glycol, glycerol, caprylocaprate.
In a preferred embodiment, the composition comprises the following components in percentage by mass:
80% -85% of oncidium essential oil, 0.1% -1.5% of linalool, 0.1% -3.0% of dihydromyrcenol, 0.05% -0.2% of phenethyl alcohol, 0.1% -2.5% of terpineol, 0.05% -0.6% of cinnamyl alcohol, 0.1% -3.5% of benzyl acetate, 0.1% -2.2% of jasmonate, 0.1% -4.5% of eugenol, 0.1% -5.5% of methyl anthranilate, 0.05% -0.4% of methyl dihydrojasmonate, 0.05% -0.5% of methyl ionone, 0.1% -1.5% of isoeugenol, 0.1% -1.5% of heliotropin, 0.1% -1.5% of dihydrojasmone, 0.1% -1.5% of linalool acetate, 0.1% -1.5% of benzyl acetate, 0.01% -0.05% of vanillin, 0.01% -0.05% of ethyl decanoate, 0.1% -10% of glycerol and 0.15% of glycerin.
On the other hand, the application also provides the application of the oncidium essential oil or the essential oil composition in preparing cigarettes.
In a preferred embodiment, the obtained oncidium essential oil prepared by the method and/or the composition can be directly added into tobacco shreds, filter sticks, cigarette paper, forming paper and/or tipping gum.
More preferably, the oncidium essential oil and/or the composition are injected into the explosion beads and then added into the filter stick of the cigarette.
The explosive beads, also called flavor drop pills, brittle capsules and the like, refer to a small liquid bead embedded in a cigarette filter tip, liquid with different types of flavors is wrapped in the small liquid bead, and the explosive bead is an emerging material in the cigarette. Before smoking, a smoker can pinch and explode the cigarette to flow out the liquid in the beads, enrich the taste of the cigarette and improve the smoking quality of the cigarette. Although a certain number and variety of bead-blasted cigarettes exist in the market at present, the flavor substances in the bead-blasted cigarettes often lack of targeted design, have poor harmony with smoke and have great influence on the aroma characteristics of the cigarettes, so that the practical application is limited.
According to the application, the oncidium essential oil and various aromatic substances are combined into the essential oil composition for the cigarette bead blasting for the first time, so that the problems of lack of targeted design and poor smoke harmony of the existing bead blasting are solved.
In another aspect, the present application also provides a pop-up bead comprising the composition described above; preferably, the explosive bead contains 10-30 μl of the composition; more preferably, the diameter of the explosion beads is 2-4 mm, the weight is 10-25 mg/granule, the roundness is less than 0.15 mm, and the crushing pressure value is 1-1.5 kgf.
In a preferred embodiment, the composition is present in the explosive beads in an amount of 10 to 25. Mu.L/particle; the diameter of the explosive beads is 2.5-3.5 mm, more preferably 2.5 mm, 2.6 mm, 3.0 mm, 3.45 mm, 3.5 mm, preferably 10.5-24.2 mg per particle, and the crushing pressure value is preferably 1.3 kgf.
In actual production operation, specific parameters such as the shape, the size, the specification, the crushing pressure value and the like of the explosion beads can be adjusted according to the specification and the size of the cigarette filter stick to which the explosion beads are added, and the explosion beads can be prepared by using tools, equipment or machines for producing corresponding specifications and the like.
In another aspect, the application also provides a cigarette, which contains the oncidium essential oil or the essential oil composition.
Preferably, the oncidium essential oil and/or the essential oil composition is/are applied to cut tobacco, filter sticks and/or cigarette paper of the cigarettes; or, the filter stick of the cigarette contains at least one explosion bead containing the oncidium essential oil and/or the essential oil composition.
In a preferred embodiment, the bead may be added to a plain cigarette, a binary composite cigarette, and/or a ternary composite cigarette; the location of the bursting beads in the cigarette filter rod is not limited, and is preferably 12-16 mm, more preferably 15 mm, from the lip end; one or more explosion beads can be added into each cigarette.
Preferably, the cigarette has a specification of not limited, and can be a thin, medium or conventional cigarette, or a common cigarette, a binary composite cigarette and/or a ternary composite cigarette, wherein the circumference of the cigarette is preferably 15-30 mm, more preferably 17 mm, 20 mm, 24 mm, 24.3 mm and 27 mm.
The application has the following beneficial effects:
1. the application provides a preparation method of oncidium essential oil suitable for cigarettes, which combines enzyme and fungus compound treatment with microwave-assisted steam distillation and molecular distillation for the first time, and can extract oncidium essential oil with high efficiency and obtain high-quality oncidium essential oil suitable for cigarettes;
2. the oncidium essential oil provided by the application can be stored for a long time, has excellent stability, can be prepared into burst beads for use in cigarettes, can effectively improve the fragrance of the cigarettes, reduce the irritation of smoke, enrich and improve the smoking feeling, obviously improve the sensory quality of the cigarettes, reduce the harm of the cigarettes, and is suitable for high-quality cigarettes.
Detailed Description
In order to more clearly illustrate the general concept of the present application, the following detailed description is given by way of example. In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present application. It will be apparent, however, to one skilled in the art that the application may be practiced without one or more of these details. In other instances, well-known features have not been described in detail in order to avoid obscuring the application.
The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer.
Wherein, hemicellulase, pectase, trypsin, acid xylanase and beta-glucanase in the enzyme preparation are all purchased from Norwestine (China) biotechnology Co., ltd; bacillus subtilis @Bacillus subtilis subsp. Subtilis) CICC24406 is purchased from China center for type culture Collection of Industrial microorganisms; preparing explosive beads by using a capsule dripping pill machine for cigarettes, which is provided by the Kataibo Xin pharmaceutical machinery Co-Ltd and has the model of DWJJ; the method comprises the steps of (1) putting explosive beads into a cigarette filter stick by adopting a JK-S1 flavor capsule input device provided by Korean positive light practical company; a cigarette was prepared using a cigarette rolling machine model ZJ17 from Changde tobacco machinery Co.Ltd.
In the following embodiments, unless specified otherwise, the reagents or apparatus used are conventional products available commercially without reference to the manufacturer.
In the following embodiments, the cigarette bursting beads are preferably prepared by a split-liquid dropping method, which includes the steps of dropping after cutting off the glue column by using the shearing force of the opposite impact of cooling oil, and specifically may be: the wall material and core liquid in the dropping process are cut off by utilizing the shearing force formed by two cooling oil with different incidence angles in the solenoid, and are condensed into balls by the action of surface tension in the cooling oil, and then the balls are manufactured into the explosion ball finished product after the procedures of deoiling, drying, screening and the like.
The sensory quality of the cigarette disclosed by the application is referred to in GB 5606.4-2005 part 4: the cigarette sensory quality judgment standard in the sensory technology requirements comprises the following steps: gloss, aroma, harmony, miscellaneous gases, irritation and aftertaste.
Example 1 Single factor experiment for extracting essential oil from Vanilla by enzyme-bacteria Complex formulation
In the embodiment, the combination of enzyme and fungus compound preparation treatment and microwave-assisted steam distillation is adopted for the first time to extract the oncidium essential oil. The specific extraction method comprises the following steps:
washing the oncidium flower petals, removing impurities, airing, and crushing to 60 meshes in liquid nitrogen to obtain oncidium powder; mixing the oncidium powder with distilled water, adding an enzyme preparation and bacillus subtilis zymotic fluid, and treating 5 h at 35 ℃ and pH of 4 to obtain oncidium treatment fluid; placing the oncidium treatment liquid in a round bottom flask, connecting a condensing device and a collecting device, performing microwave-assisted steam distillation under normal pressure, setting the microwave extraction power to be 250W, collecting distillate after distillation and extraction for 70 min, separating the distillate in a separating funnel, and reserving the upper layer fraction to obtain oncidium essential oil, wherein the extraction time is calculated according to the time that the content of the oncidium essential oil is not increased, and the rest conditions are shown in Table 2.
The preparation method of the bacillus subtilis bacterial liquid comprises the following steps: inoculating Bacillus subtilis CICC24406 into 50 mL LB liquid medium, culturing at 37deg.C and 150 r/min to OD 600 When the value is 3-4 (about 48 h), the biological activity of the bacillus subtilis in the bacterial liquid is higher.
The extraction rate of the oncidium essential oil is calculated as shown in the following table 2.
Wherein: y is the extraction rate of the oncidium essential oil,%; m is the mass of dried oncidium, g; m is m 1 G, for receiving the mass of the dish; m is m 2 G for receiving the quality of the dish and the oncidium essential oil.
And, 10 evaluators evaluate the fragrance of the obtained oncidium essential oil at the detection port of the sniffer, the fragrance intensity is recorded, the intensity is specifically classified into four grades of 0-3, the final score is obtained by comprehensive score of the evaluators, the specific intensity grade is shown in table 1, and the test result is shown in table 2.
TABLE 1
TABLE 2
Note that: the enzyme preparation proportion is the enzyme activity ratio; the addition amount of the enzyme preparation and the addition amount of the bacterial liquid are the addition amount of the oncidium per 100 g.
From the results in table 2, it is demonstrated in this example that the combination of enzyme and bacterial complex treatment and microwave assisted steam distillation can be used to extract the essential oil of oncidium.
Preferably, hemicellulase, pectase, trypsin, acid xylanase and beta-glucanase with the enzyme activity ratio of 5:3:3:2:4 are selected as enzyme preparations, the addition amount of the hemicellulase, pectase, trypsin, acid xylanase and beta-glucanase is 1% of the mass of the powder of the oncidium, the addition amount of the bacillus subtilis zymotic bacteria liquid is 0.1% of the mass of the powder of the oncidium, and when the microwave-assisted steam distillation collection temperature is 100 ℃, the extraction rate of the oncidium essential oil is highest, the extraction time is relatively short, and the odor intensity is high.
Example 2 Single factor experiment for extracting essential oil of oncidium by molecular distillation
However, in the actual operation process, the essential oil aroma prepared in the embodiment 1 is still difficult to mask smoke when being applied to cigarettes, and the aroma is not strong enough. In addition, the oncidium essential oil has the problem of poor stability during long-term storage, and a method for preparing a bead explosion product stably stored in a growth period is unavailable.
The molecular distillation method was selected in this example to solve the above-mentioned problems. The molecular distillation treatment is continued on the basis of the preferred conditions of the example 1 to obtain the high-quality oncidium essential oil, and the specific operation comprises the following steps:
the product under the preferred conditions of the embodiment 1 is added into molecular distillation equipment for primary molecular distillation and secondary molecular distillation, wherein the primary molecular distillation is carried out under the conditions of different distillation pressures and distillation temperatures, the sample injection temperature is 60 ℃, the condensation temperature is 25 ℃, the secondary molecular distillation is carried out under the conditions of different distillation pressures and distillation temperatures, the sample injection temperature is 50 ℃, the condensation temperature is 60 ℃, and the primary molecular distillation light component and the secondary molecular distillation light component are combined, so that the high-quality oncidium essential oil is obtained. The odor intensity was determined as described in example 1. The stability of the oncidium was measured by allowing the oncidium essential oil to stand at normal temperature and pressure for 1 month, and the system stability was observed, and the observation results are shown in table 3.
TABLE 3 Table 3
As can be seen from the results in table 3, in this example, it is demonstrated that in the process of extracting the essential oil from the oncidium by using the enzyme-bacteria complex preparation in combination with the microwave-assisted steam distillation method and the molecular distillation method, the optimal preparation condition in the molecular distillation method is that the first-order molecular distillation is performed under the conditions of a distillation pressure of 5 Pa, a sample injection temperature of 60 ℃, a distillation temperature of 130 ℃, a condensation temperature of 25 ℃, and a second-order molecular distillation is performed under the conditions of a distillation pressure of 5 Pa, a sample injection temperature of 50 ℃, a distillation temperature of 160 ℃, and a condensation temperature of 60 ℃. The obtained oncidium essential oil has good stability, strong fragrance, obvious cream sweet fragrance and suitability for preparing cigarette pop beads.
Example 3 experiment for physical and chemical Properties detection and sensory quality evaluation of cigarette
Test example 1
The experimental example provides a preparation method of the oncidium essential oil, which comprises the following steps:
washing the oncidium petals, removing impurities, airing, and crushing in liquid nitrogen to 60 meshes to obtain oncidium powder;
mixing the oncidium powder and distilled water according to a mass ratio of 1:10, stirring to form slurry, adding 1 g enzyme preparation and 100 mu L of bacillus subtilis bacterial liquid into each 100g oncidium powder, wherein the enzyme preparation is a compound enzyme containing hemicellulase, pectase, trypsin, acid xylanase and beta-glucanase with an enzyme activity ratio of 5:3:3:2:4, and treating 5 h under the conditions that the temperature is 35 ℃ and the pH value is 4 to obtain oncidium treatment liquid;
placing the oncidium treatment liquid in a round bottom flask, connecting a condensing device and a collecting device, performing microwave-assisted steam distillation under normal pressure, setting the microwave extraction power to be 250W, observing that oily fractions are distilled when the oncidium treatment liquid is heated to 100 ℃, collecting distillate after distillation and extraction for 70 min, separating the distillate in a separating funnel, and reserving the upper fraction to obtain the oncidium crude extract;
and step four, adding the crude extract of the oncidium into molecular distillation equipment to carry out primary and secondary molecular distillation, wherein the primary molecular distillation is carried out under the conditions of a distillation pressure of 5 Pa, a sample injection temperature of 60 ℃, a distillation temperature of 130 ℃ and a condensation temperature of 25 ℃, the secondary molecular distillation is carried out under the conditions of a distillation pressure of 5 Pa, a sample injection temperature of 50 ℃, a distillation temperature of 160 ℃ and a condensation temperature of 60 ℃, and the primary molecular distillation light component and the secondary molecular distillation light component are combined and inactivated at 100 ℃ for 5 min to obtain the high-quality oncidium essential oil.
The bacillus subtilis is bacillus subtilis [ (]Bacillus subtilis subsp. Subtilis) CICC24406, which is purchased from China center for type culture Collection of microorganisms.
The preparation method of the bacillus subtilis bacterial liquid comprises the following steps: inoculating bacillus subtilis CICC24406 into50 mL LB liquid medium, 37 ℃,150 r/min, 48 h of culture, OD 600 The value is 3.
The composition containing the oncidium essential oil comprises the following components in percentage by mass:
85% of oncidium essential oil, 0.5% of linalool, 0.5% of dihydromyrcenol, 0.1% of phenethyl alcohol, 0.5% of terpineol, 0.1% of cinnamyl alcohol, 0.5% of benzyl acetate, 0.5% of jasmonate, 0.5% of eugenol, 0.5% of methyl anthranilate, 0.1% of methyl dihydrojasmonate, 0.1% of methyl ionone, 0.1% of isoeugenol, 0.1% of heliotropin, 0.1% of dihydrojasmone, 0.1% of linalool acetate, 0.1% of benzyl acetate, 0.05% of vanillin, 0.01% of ethyl vanillin, 0.2% of propylene glycol, 5.17% of glycerol and 5.17% of caprylic and capric glyceride.
A bursting bead comprising the composition, prepared by using the split-liquid drop method. Wherein the content of the composition in the explosive beads is 15 mu L/grain, the diameter of the explosive beads is 3 mm, the weight of the explosive beads is 20 mg/grain, the roundness is less than 0.15 mm, and the crushing pressure value is 1.3 kgf.
The cigarette containing the explosion beads is prepared according to the existing cigarette production process. The cigarette is a common cigarette, a circumference 17, mm, a burst bead is placed in a cigarette filter stick, and the burst bead is placed in the middle section of the cigarette filter stick, specifically 15.0 mm away from a lip end.
Test example 2
The test example differs from test example 1 only in that no enzymatic treatment was performed.
Test example 3
The test example differs from test example 1 only in that no bacterial liquid was added.
Test example 4
The test example differs from test example 1 only in that the microwave-assisted steam distillation was replaced by molecular ultrasound-assisted steam distillation.
Test example 5
The test example differs from test example 1 only in that molecular petroleum ether leaching is used instead of molecular distillation.
Comparative example 1
This comparative example differs from test example 1 only in that the composition does not include the oncidium essential oil.
Cigarette physicochemical property detection experiment
The cigarettes of test examples 1 to 5 and comparative example 1 were measured for the content of harmful components in mainstream smoke before and after burst beads were not broken, respectively, and the cigarette hazard evaluation index, i.e., H value (two decimal places) was calculated, and the specific results obtained are shown in table 4.
The calculation method of the cigarette hazard evaluation index (H value) is shown in the following formula.
Wherein: h is a cigarette hazard evaluation index; x is X CO The measured value of the release amount of CO in the main stream smoke of the cigarette; x is X HCN The measured value of the HCN release amount in the main stream smoke of the cigarettes; x is X NH3 Is NH in main stream smoke of cigarette 3 A measured value of the release amount; x is X B[a]P Benzo [ a ] in main stream smoke of cigarette]Actual measurement of pyrene release amount; x is X Phenol (P) Is the actual measurement value of the release amount of phenol in the main stream smoke of the cigarettes; x is X Crotonaldehyde Is the measured value of the release amount of crotonaldehyde in the main stream smoke of cigarettes; x is X NNK Is the actual measurement value of NNK release amount in main stream smoke of cigarettes.
Table 4 evaluation of cigarette hazard in each example
From the data in Table 4, the cigarettes of test example 1 were significantly reduced in the presence of seven harmful components before and after bead bursting.
The conclusion shows that the cigarette containing the composition taking the oncidium essential oil as the main component can effectively reduce the harmful components of main stream smoke generated by burning tobacco during smoking, improve the quality of the cigarette and reduce the harm to human health.
Sensory quality evaluation experiment
33 professional sensory evaluators were randomly drawn to perform sensory quality evaluation on cigarettes obtained in each example. The sensory quality of the cigarette disclosed by the application is referred to in GB 5606.4-2005 part 4: the cigarette sensory quality judgment standard in the sensory technology requirements comprises the following steps: the gloss, fragrance, harmony, miscellaneous odor, irritation and aftertaste, and specific evaluation criteria are shown in Table 5.
Table 5 criteria for judging the sensory quality of cigarettes
The sensory quality of each example cigarette was scored by a professional sensory evaluator according to the sensory quality evaluation criteria of the cigarettes in table 5, and the average value was taken as the final score, and the sensory quality evaluation scoring results of each example cigarette are shown in table 6.
Table 6 sensory quality evaluation of cigarettes obtained in each example
As can be seen from the data in table 6, the cigarettes obtained in test example 1 have a higher multiple score in sensory quality evaluation than those of comparative example 1, indicating that the sensory quality of the cigarettes according to the embodiment of the application is significantly improved. As can be seen from the specific feedback description of the professional sensory evaluator, the sensory feel of the cigarette obtained in test example 1 during smoking is mainly represented by: the richness, fineness and thickness of the smoke are obviously improved, the smoke is more intense and is thoroughly emitted, the fusion degree is better, the fragrance of flower fragrance, sweet fragrance and fruit fragrance is prominent, no stimulation and impurity gas are generated, the harmony is good, the smoke fineness is high, the mouth is soft, the sweet taste is obviously enhanced, and the aftertaste is still clean.
The conclusion shows that when the pop beads prepared from the composition taking the oncidium essential oil as the main component provided by the application are applied to cigarettes, the sensory quality of the cigarettes can be obviously improved, the fragrance is improved, the aftertaste is improved, the irritation and miscellaneous gases of the cigarettes are obviously reduced, and the strong and unique oncidium fragrance and sweetness are shown, which are not possessed by the cigarettes containing other additives.
The foregoing is merely exemplary of the present application and is not intended to limit the present application. Various modifications and variations of the present application will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the application are to be included in the scope of the claims of the present application.
Claims (10)
1. A method for preparing a oncidium essential oil, comprising the steps of:
crushing the oncidium petals to 40-100 meshes to obtain oncidium powder;
step two, mixing the oncidium powder with water, adding an enzyme preparation and bacillus subtilis bacterial liquid, and treating 2-8 h at the temperature of 30-60 ℃ and the pH value of 4-7 to obtain oncidium treatment liquid; the enzyme preparation is selected from one or more of hemicellulase, pectinase, trypsin, acid xylanase and beta-glucanase;
performing microwave-assisted steam distillation on the oncidium treatment liquid, heating to 80-100 ℃ with microwave extraction power of 250W, distilling and extracting for 30-90 min, collecting distillate for separating liquid, and reserving upper-layer fractions to obtain a oncidium crude extract;
and step four, carrying out primary and secondary molecular distillation on the crude extract of the oncidium, and merging the primary molecular distillation light component and the secondary molecular distillation light component to obtain the oncidium essential oil.
2. The preparation method according to claim 1, wherein the enzyme preparation is hemicellulase, pectase, trypsin, acid xylanase and beta-glucanase with an enzyme activity ratio of (1-9): (2-6): (2-5): (2-5): (1-5).
3. The preparation method of claim 1, wherein the enzyme preparation is added in an amount of 1-1.5% of the mass of the powder of the vetiver, and the bacillus subtilis liquid is added in an amount of 0.1-0.5% of the mass of the powder of the vetiver.
4. The preparation method of claim 1, wherein the bacillus subtilis in the bacillus subtilis liquid is bacillus subtilis CICC24406 and bacillus subtilis liquid OD 600 The value is 3-4.
5. The preparation method according to claim 1, wherein the mass ratio of the oncidium powder to the water is 1 (8-20).
6. The process of claim 1, wherein the primary molecular distillation is carried out at a distillation pressure of 0.1 to 10 Pa, a sample introduction temperature of 60 ℃ to 70 ℃, a distillation temperature of 100 ℃ to 130 ℃, a condensation temperature of 20 ℃ to 40 ℃, a distillation pressure of 0.1 to 10 Pa, a sample introduction temperature of 50 ℃ to 70 ℃, a distillation temperature of 150 ℃ to 170 ℃, and a condensation temperature of 60 ℃ to 70 ℃.
7. The oncidium essential oil prepared by the method as claimed in any one of claims 1-6.
8. An essential oil composition, wherein the essential oil composition comprises the oncidium essential oil of claim 7.
9. Use of the oncidium essential oil of claim 7 or the essential oil composition of claim 8 in the preparation of cigarettes.
10. A cigarette comprising the oncidium essential oil of claim 7 or the essential oil composition of claim 8.
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| CN106262654A (en) * | 2016-08-11 | 2017-01-04 | 广州市名花香料有限公司 | Cheese powdered flavor and preparation method thereof |
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