CN116584485A - Sterilization composition for preventing and treating gray mold - Google Patents
Sterilization composition for preventing and treating gray mold Download PDFInfo
- Publication number
- CN116584485A CN116584485A CN202310455929.2A CN202310455929A CN116584485A CN 116584485 A CN116584485 A CN 116584485A CN 202310455929 A CN202310455929 A CN 202310455929A CN 116584485 A CN116584485 A CN 116584485A
- Authority
- CN
- China
- Prior art keywords
- famoxadone
- agent
- difenoconazole
- thiophanate
- boscalid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 25
- 230000001954 sterilising effect Effects 0.000 title description 3
- 238000004659 sterilization and disinfection Methods 0.000 title description 3
- 239000005760 Difenoconazole Substances 0.000 claims abstract description 28
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 claims abstract description 27
- PCCSBWNGDMYFCW-UHFFFAOYSA-N 5-methyl-5-(4-phenoxyphenyl)-3-(phenylamino)-1,3-oxazolidine-2,4-dione Chemical compound O=C1C(C)(C=2C=CC(OC=3C=CC=CC=3)=CC=2)OC(=O)N1NC1=CC=CC=C1 PCCSBWNGDMYFCW-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000005740 Boscalid Substances 0.000 claims abstract description 26
- 239000005772 Famoxadone Substances 0.000 claims abstract description 26
- 239000005842 Thiophanate-methyl Substances 0.000 claims abstract description 26
- 229940118790 boscalid Drugs 0.000 claims abstract description 26
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 25
- WYEMLYFITZORAB-UHFFFAOYSA-N boscalid Chemical compound C1=CC(Cl)=CC=C1C1=CC=CC=C1NC(=O)C1=CC=CN=C1Cl WYEMLYFITZORAB-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000006013 carbendazim Substances 0.000 claims abstract description 25
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical compound COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims abstract description 25
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 24
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000004480 active ingredient Substances 0.000 claims abstract description 10
- 239000003899 bactericide agent Substances 0.000 claims abstract description 10
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 28
- 240000003768 Solanum lycopersicum Species 0.000 claims description 28
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 240000008067 Cucumis sativus Species 0.000 claims description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 3
- 208000031888 Mycoses Diseases 0.000 claims description 3
- 241000209140 Triticum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 239000012752 auxiliary agent Substances 0.000 claims description 3
- 241000221785 Erysiphales Species 0.000 claims description 2
- 241000233679 Peronosporaceae Species 0.000 claims description 2
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 2
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 2
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 2
- 239000002518 antifoaming agent Substances 0.000 claims description 2
- 239000011230 binding agent Substances 0.000 claims description 2
- 239000002270 dispersing agent Substances 0.000 claims description 2
- 239000003995 emulsifying agent Substances 0.000 claims description 2
- 239000000945 filler Substances 0.000 claims description 2
- 230000000855 fungicidal effect Effects 0.000 claims description 2
- 239000000417 fungicide Substances 0.000 claims description 2
- 239000003755 preservative agent Substances 0.000 claims description 2
- 230000002335 preservative effect Effects 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 239000003381 stabilizer Substances 0.000 claims description 2
- 239000002562 thickening agent Substances 0.000 claims description 2
- 239000000080 wetting agent Substances 0.000 claims description 2
- 206010017533 Fungal infection Diseases 0.000 claims 1
- 241000219095 Vitis Species 0.000 claims 1
- 230000009418 agronomic effect Effects 0.000 claims 1
- 208000024386 fungal infectious disease Diseases 0.000 claims 1
- 244000052616 bacterial pathogen Species 0.000 abstract description 12
- 239000000575 pesticide Substances 0.000 abstract description 8
- 230000002195 synergetic effect Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 6
- 230000002401 inhibitory effect Effects 0.000 abstract description 5
- 206010059866 Drug resistance Diseases 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 239000000447 pesticide residue Substances 0.000 abstract description 4
- 238000013329 compounding Methods 0.000 abstract description 3
- ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Chemical compound C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 description 32
- -1 sesquiterpene lactone compound Chemical class 0.000 description 24
- 229940074386 skatole Drugs 0.000 description 16
- 239000003814 drug Substances 0.000 description 10
- 241000123650 Botrytis cinerea Species 0.000 description 9
- 150000001875 compounds Chemical class 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 8
- 230000004071 biological effect Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- 230000001018 virulence Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 239000013043 chemical agent Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 206010034133 Pathogen resistance Diseases 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000223600 Alternaria Species 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 235000000832 Ayote Nutrition 0.000 description 1
- 241001290235 Ceratobasidium cereale Species 0.000 description 1
- 239000005747 Chlorothalonil Substances 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 239000005759 Diethofencarb Substances 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000005867 Iprodione Substances 0.000 description 1
- 241000233616 Phytophthora capsici Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- HATRDXDCPOXQJX-UHFFFAOYSA-N Thapsigargin Natural products CCCCCCCC(=O)OC1C(OC(O)C(=C/C)C)C(=C2C3OC(=O)C(C)(O)C3(O)C(CC(C)(OC(=O)C)C12)OC(=O)CCC)C HATRDXDCPOXQJX-UHFFFAOYSA-N 0.000 description 1
- 244000272739 Vitis cinerea Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- LNJNFVJKDJYTEU-UHFFFAOYSA-N diethofencarb Chemical compound CCOC1=CC=C(NC(=O)OC(C)C)C=C1OCC LNJNFVJKDJYTEU-UHFFFAOYSA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- ONUFESLQCSAYKA-UHFFFAOYSA-N iprodione Chemical compound O=C1N(C(=O)NC(C)C)CC(=O)N1C1=CC(Cl)=CC(Cl)=C1 ONUFESLQCSAYKA-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- QXJKBPAVAHBARF-BETUJISGSA-N procymidone Chemical compound O=C([C@]1(C)C[C@@]1(C1=O)C)N1C1=CC(Cl)=CC(Cl)=C1 QXJKBPAVAHBARF-BETUJISGSA-N 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 229930009674 sesquiterpene lactone Natural products 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating gray mold. The bactericidal composition is prepared by compounding the active ingredients of the famoxadone with thiophanate-methyl, boscalid, carbendazim or difenoconazole, wherein the mass ratio of the famoxadone to the thiophanate-methyl, boscalid, carbendazim or difenoconazole is 1-100:100-1. The invention has good synergistic effect on inhibiting the growth of gray mold pathogenic bacteria hypha after the active ingredient of the famoxadone is compounded with thiophanate-methyl, boscalid, carbendazim or difenoconazole, and compared with the single active ingredient, the invention can improve the control effect on gray mold, is beneficial to reducing the pesticide application dosage, delaying the generation of pathogenic bacteria drug resistance, reducing pesticide residues, ensuring the yield and quality, and can provide support for developing a novel bactericide for efficiently controlling gray mold.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating gray mold.
Background
The gray mold of the tomato is a disease caused by Botrytis cinerea (Botrytis cinerea), mainly damages fruits, can damage parts such as leaves and stems, can cause rotten seedlings, rotten fruits and the like, and seriously affects the yield and quality of the tomato. Because of the lack of gray mold resistant varieties of tomatoes, the control of gray mold of tomatoes still takes chemical agents as main materials, and pesticides registered in the control of gray mold of tomatoes in China mainly comprise procymidone, chlorothalonil, iprodione, carbendazim, thiophanate-methyl, diethofencarb and the like. Although the chemical agent can rapidly and effectively prevent and treat diseases, the problems of pesticide residues, pathogen resistance, environmental pollution and the like are increasingly prominent due to unscientific continuous use of a large amount of chemical agents, wherein the prevention and treatment effect is reduced year by year due to the pathogen resistance. In order to delay the development of drug resistance of pathogenic bacteria and ensure the yield and quality of tomatoes, development of a novel bactericide for efficiently preventing and treating gray mold is urgently needed.
The skyloctone is sesquiterpene lactone compound widely existing in plant of genus skyloctonia of family Compositae, has distribution in root, fruit and aerial parts of plant of genus skyloctonia, has been separated from 3 plants of genus skylocrataegus, herba Alternantherae sessilis and genus skylocrataegus, and has molecular formula C 15 H 20 O 3 Colorless prismatic crystals (ethyl acetate) having the following structural formula:
the antibacterial activity of the TIANMINGGANOTONGZONE (Han Xingshuai et al, university of North agriculture and forestry science and technology, journal of 2014, volume 42, 8) discloses that TIANMINGGANOTONGONE has antibacterial activity against Rhizoctonia cerealis, wheat take-off pathogen, alternaria aestiva, phytophthora capsici, apple anthracnoseThe bacterial strain, cucumber anthracnose, apple dry rot, pumpkin fusarium wilt, tomato gray mold and tomato leaf mold mycelium growth have stronger inhibition effect, and the EC50 is 4.8947-43.8569mg/L, wherein the EC on the tomato gray mold is that 50 30.5520mg/L.
The compounding of pesticide active ingredients with different action mechanisms is an effective and quick way for developing new pesticide products and preventing and controlling pest and disease resistance at present. The applicant entrusts the teacher of the Guangxi Zhuang national academy of agricultural sciences to compound and screen the medicament for preventing and treating the gray mold of tomatoes, and finds that synergy is generated when the famoxadone is compounded with thiophanate-methyl, boscalid, carbendazim or difenoconazole, and at present, no related report of the compounding of the famoxadone with thiophanate-methyl, boscalid, carbendazim or difenoconazole is yet seen.
Disclosure of Invention
The invention aims to provide a bactericidal composition for preventing and treating gray mold, which has a synergistic effect on inhibiting the growth of pathogenic bacterial hyphae of the gray mold of tomatoes when active components are compounded, can improve the preventing and treating effect on the gray mold of tomatoes, and can provide support for developing a novel bactericide for efficiently preventing and treating the gray mold.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the first aim of the invention is to provide a sterilization composition, the active ingredients of which are compounded by famoxadone and thiophanate-methyl, boscalid, carbendazim or difenoconazole, wherein the mass ratio of the famoxadone to the thiophanate-methyl, boscalid, carbendazim or difenoconazole is 1-100:100-1.
Preferably, the mass ratio of the famoxadone to the thiophanate-methyl is 1-39:15-1.
Preferably, the mass ratio of the famoxadone to the boscalid is 1-9:40-1.
Preferably, the mass ratio of the famoxadone to the carbendazim is 1-10:10-1.
Preferably, the mass ratio of the famoxadone to the difenoconazole is 1-60:19-1.
A second object of the present invention is to provide a bactericide which is prepared from the bactericidal composition of claim 1 and an agronomically acceptable auxiliary agent consisting of one or more of a filler, a wetting agent, an emulsifying agent, a binder, a disintegrant, a dispersant, a thickener, a preservative, an antifoaming agent, an antifreezing agent, a solvent and a stabilizer.
Preferably, the active ingredient accounts for 0.75-75% of the total mass of the bactericide.
A third object of the present invention is to provide the use of the fungicidal composition or the fungicidal agent for controlling fungal diseases of crops.
Preferably, the crop fungal disease includes tomato gray mold, tomato anthracnose, wheat powdery mildew, cucumber downy mildew and grape anthracnose.
Compared with the prior art, the invention has the following beneficial effects:
the invention has good synergistic effect on inhibiting the growth of the pathogenic bacteria hyphae of the gray mold of the tomato after the active ingredient of the famoxadone is compounded with the thiophanate-methyl, the boscalid, the carbendazim or the difenoconazole, and compared with the single active ingredient, the invention can improve the control effect on the gray mold of the tomato, is beneficial to reducing the pesticide application dosage, delaying the generation of the drug resistance of the pathogenic bacteria, reducing the pesticide residue in the tomato, ensuring the yield and the quality of the tomato, and can provide support for developing a novel bactericide for efficiently controlling the gray mold.
Detailed Description
The invention will be better understood from the following examples. However, it will be readily appreciated by those skilled in the art that the description of the embodiments is provided for illustration only and should not limit the invention as described in detail in the claims.
Examples: nannochloride ketone compound indoor biological activity test
1. Test strain: collecting tomato gray mold disease leaves in a tomato test base of Guangxi Zhuang national academy of agricultural sciences, separating and purifying in a laboratory, identifying pathogenic bacteria as Botrytis cinerea, and storing in PDA slant culture medium for use.
Pda medium: 200g of peeled potatoes, 20g of glucose, 18g of agar and 1000mL of distilled water.
3. Test agent
98% of the crude drug of Nannochloride ketone (Shanghai Yuan leaf Biotechnology Co., ltd.)
95% thiophanate-methyl (Anhui Guangxin Agrochemical Co., ltd.)
98% boscalid (Ningxia Gri Fine chemical Co., ltd.)
98% carbendazim (Jiangsu Huifeng biological agriculture Co., ltd.)
95% difenoconazole technical (Zhejiang Tianfeng bioscience Co., ltd.)
Dissolving each test agent with dimethyl sulfoxide, diluting with 0.1% Tween-80 to obtain single-agent mother solution, and setting multiple groups of ratios, wherein each single-agent and each group of ratio mixture have 5 mass concentration gradients according to an equal ratio method for use.
4. Test method (refer to "NY/T1156.2-2006 laboratory biological assay criteria for Agents section 2: petri dish method for inhibiting growth of pathogenic fungi)
Under the aseptic condition, adding 9mL of pre-melted PDA culture medium into an aseptic conical flask, sequentially quantitatively sucking 1mL of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, fully shaking uniformly, pouring into a culture dish with the diameter of 9cm, and preparing a medicine-containing flat plate with corresponding concentration; treatments with 0.1% tween-80 in water were used as blank, and 10 replicates were set for each treatment.
Cutting bacterial cake at the colony edge of the tested bacterial strain by using a puncher with the diameter of 5mm, inoculating the bacterial cake to the centers of a medicine-containing flat plate and a blank control flat plate, enabling the mycelium surface to face upwards, covering a dish cover, placing the dish cover in a constant temperature cabinet at 25 ℃ for culturing for 4d, measuring the colony diameter by using a crisscross method, and calculating the mycelium growth inhibition rate of different treatments.
5. Data analysis: performing data statistical analysis by DPS software, and linearly regressing with the value of the disinfectant concentration logarithmic value x and the value of the corresponding hypha growth inhibition rate logarithmic value y to obtain a virulence regression equation and the virulence EC of the medicament on the target pathogen 50 Values and co-toxicity coefficients (CTCs) were calculated according to the grand cloud Pei method.
In the above formula: ati—the measured virulence index of the mixture; s- -EC of standard pharmaceutical agent 50 The unit is mg/L; m- -EC of mixture 50 The unit is mg/L.
TTI＝TI A ×P A +TI B ×P B
In the above formula: TTI- -theoretical toxicological index of the mixture; TI (TI) A -a toxicity index of the agent; p (P) A - -A the percentage of agent in the blend in percent (%); TI (TI) B -a toxicity index of the agent; p (P) B The percentage content of the agent in the mixture is shown as percentage (%).
In the above formula: ctc—co-toxicity coefficient; ati—actual measured virulence index of the mixture; tti—the theoretical toxicity index of the mixture.
6. Measurement results
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-4.
Table 1 determination of indoor biological Activity of famoxadone and thiophanate-methyl Complex against Botrytis cinerea pathogen
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Nannochloride lactone ketone | 43.77 | 100.00 | -- | -- |
| Thiophanate-methyl | 19.31 | 226.67 | -- | -- |
| Nannochloride lactone ketone 1: thiophanate-methyl 15 | 14.03 | 311.97 | 218.75 | 142.61 |
| Nannochloride lactone ketone 1: thiophanate-methyl 7 | 11.49 | 380.94 | 210.84 | 180.68 |
| Nannochloride lactone ketone 1: thiophanate-methyl3 | 8.44 | 518.60 | 195.00 | 265.95 |
| Nannochloride lactone ketone 1: thiophanate-methyl 1 | 17.92 | 244.25 | 163.34 | 149.54 |
| Skatole 3: thiophanate-methyl 1 | 22.04 | 198.59 | 131.67 | 150.83 |
| Skatole 7: thiophanate-methyl 1 | 25.87 | 169.19 | 115.83 | 146.06 |
| Skatole 15: thiophanate-methyl 1 | 31.21 | 140.24 | 107.92 | 129.96 |
| Skatole 30: thiophanate-methyl 1 | 22.48 | 194.71 | 104.09 | 187.06 |
| Skatole 39: thiophanate-methyl 1 | 26.50 | 165.17 | 103.17 | 160.10 |
As shown in Table 1, after the compound of the famoxadone and the thiophanate-methyl, the mass ratio is 1-39: the co-toxicity coefficients of the total bacterial strain and the total bacterial strain of the botrytis cinerea are more than 120 in 15-1, and the total bacterial strain show synergistic effect.
Table 2 determination of indoor biological Activity of famoxadone and boscalid in combination against Botrytis cinerea pathogen
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Nannochloride lactone ketone | 43.77 | 100.00 | -- | -- |
| Boscalid | 3.29 | 1330.40 | -- | -- |
| Nannochloride lactone ketone 1: boscalid 40 | 2.61 | 1677.01 | 1300.39 | 128.96 |
| Nannochloride lactone ketone 1: boscalid 30 | 2.32 | 1886.64 | 1290.70 | 146.17 |
| Nannochloride lactone ketone 1: boscalid 15 | 1.99 | 2199.50 | 1253.50 | 175.47 |
| Nannochloride lactone ketone 1: boscalid 7 | 1.34 | 3266.42 | 1176.60 | 277.62 |
| Nannochloride lactone ketone 1: boscalid 3 | 1.16 | 3773.28 | 1022.80 | 368.92 |
| Nannochloride lactone ketone 1: boscalid 1 | 2.70 | 1621.11 | 715.20 | 226.67 |
| Skatole 3: boscalid 1 | 3.52 | 1243.47 | 407.60 | 305.07 |
| Skatole 7: boscalid 1 | 4.85 | 902.47 | 253.80 | 355.59 |
| Skatole 9: boscalid 1 | 6.89 | 635.27 | 223.04 | 284.82 |
As can be seen from table 2, after the compounded of the famoxadone and boscalid, the mass ratio is 1-9: the co-toxicity coefficient of the compound in 40-1 to the pathogenic bacteria of the gray mold of the tomato is more than 120, and the compound has synergistic effect.
Table 3 determination of indoor biological Activity of famoxadone and carbendazim complex against Botrytis cinerea pathogenic bacteria
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Nannochloride lactone ketone | 43.77 | 100.00 | -- | -- |
| Carbendazim | 8.39 | 521.69 | -- | -- |
| Nannochloride lactone ketone 1: carbendazim 10 | 7.39 | 592.29 | 483.36 | 122.54 |
| Nannochloride lactone ketone 1: carbendazim 7 | 6.54 | 669.27 | 468.98 | 142.71 |
| Tianming essenceEster ketone 1: carbendazim 3 | 4.09 | 1070.17 | 416.27 | 257.09 |
| Nannochloride lactone ketone 1: carbendazim 1 | 9.25 | 473.19 | 310.85 | 152.23 |
| Skatole 3: carbendazim 1 | 3.12 | 1402.88 | 205.42 | 682.92 |
| Skatole 7: carbendazim 1 | 5.35 | 818.13 | 152.71 | 535.74 |
| Skatole 10: carbendazim 1 | 4.18 | 1047.13 | 138.34 | 756.95 |
As shown in Table 3, after the compound of the famoxadone and the carbendazim, the mass ratio is 1-10: the co-toxicity coefficient of the compound in 10-1 to the pathogenic bacteria of the botrytis cinerea is more than 120, and the compound has synergistic effect.
Table 4 determination of indoor biological Activity of famoxadone and difenoconazole Complex against Botrytis cinerea pathogen
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Nannochloride lactone ketone | 43.77 | 100.00 | -- | -- |
| Difenoconazole | 1.46 | 2997.95 | -- | -- |
| Nannochloride lactone ketone 1: difenoconazole 19 | 0.93 | 4706.45 | 2853.05 | 164.96 |
| Nannochloride lactone ketone 1: difenoconazole 15 | 1.12 | 3908.04 | 2816.82 | 138.74 |
| Nannochloride lactone ketone 1: difenoconazole 7 | 0.54 | 8105.56 | 2635.70 | 307.53 |
| Nannochloride lactone ketone 1: difenoconazole 3 | 1.45 | 3018.62 | 2273.46 | 132.78 |
| Nannochloride lactone ketone 1: difenoconazole 1 | 1.73 | 2530.06 | 1548.97 | 163.34 |
| Skatole 3: difenoconazole 1 | 3.33 | 1314.41 | 824.49 | 159.42 |
| Skatole 7: difenoconazole 1 | 5.49 | 797.27 | 462.24 | 172.48 |
| Skatole 15: difenoconazole 1 | 9.16 | 477.84 | 281.12 | 169.98 |
| Skatole 30: difenoconazole 1 | 10.67 | 410.22 | 193.48 | 212.02 |
| Skatole 60: difenoconazole 1 | 16.45 | 266.08 | 147.51 | 180.38 |
As shown in Table 4, after the compound of the famoxadone and the difenoconazole, the mass ratio is 1-60: the co-toxicity coefficients of the 19-1 to the tomato gray mold pathogenic bacteria are all more than 120, and the synergistic effect is shown.
In conclusion, after being compounded with thiophanate-methyl, boscalid, carbendazim or difenoconazole, the fenhexamid-methyl and difenoconazole have a good synergistic effect on inhibiting the growth of pathogenic bacteria hyphae of gray mold of tomatoes, compared with single effective components, the compound preparation has the advantages of improving the control effect on gray mold of tomatoes, being beneficial to reducing the pesticide application dosage, delaying the generation of drug resistance of pathogenic bacteria, reducing pesticide residues in tomatoes, ensuring the yield and quality of tomatoes and providing support for developing novel bactericides for efficiently controlling gray mold.
The foregoing is merely illustrative of the preferred embodiments of this invention and modifications, obvious to those skilled in the art, may be made without departing from the principles of this invention and are within the scope of this invention.
Claims (9)
1. The bactericidal composition is characterized in that the active ingredients of the bactericidal composition are compounded by famoxadone and thiophanate-methyl, boscalid, carbendazim or difenoconazole, and the mass ratio of the famoxadone to the thiophanate-methyl, boscalid, carbendazim or difenoconazole is 1-100:100-1.
2. The bactericidal composition of claim 1, wherein the mass ratio of the famoxadone to thiophanate-methyl is 1-39:15-1.
3. The bactericidal composition of claim 1, wherein the mass ratio of famoxadone to boscalid is 1-9:40-1.
4. The bactericidal composition of claim 1, wherein the mass ratio of the famoxadone to the carbendazim is 1-10:10-1.
5. The bactericidal composition of claim 1, wherein the mass ratio of the famoxadone to difenoconazole is 1-60:19-1.
6. The bactericide is characterized by being prepared from the bactericidal composition of claim 1 and an auxiliary agent acceptable in agronomic science, wherein the auxiliary agent consists of one or more of a filler, a wetting agent, an emulsifying agent, a binder, a disintegrating agent, a dispersing agent, a thickening agent, a preservative, a defoaming agent, an antifreezing agent, a solvent and a stabilizing agent.
7. The bactericide according to claim 6, characterized in that the active ingredient accounts for 0.75-75% of the total mass of the bactericide.
8. Use of a fungicidal composition according to any one of claims 1 to 5 or a fungicidal agent according to any one of claims 6 to 7 for controlling fungal diseases of crops.
9. The use according to claim 8, characterized in that the crop mycosis diseases include tomato gray mold, tomato anthracnose, wheat powdery mildew, cucumber downy mildew and grape anthracnose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310455929.2A CN116584485A (en) | 2023-04-25 | 2023-04-25 | Sterilization composition for preventing and treating gray mold |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310455929.2A CN116584485A (en) | 2023-04-25 | 2023-04-25 | Sterilization composition for preventing and treating gray mold |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116584485A true CN116584485A (en) | 2023-08-15 |
Family
ID=87589049
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310455929.2A Pending CN116584485A (en) | 2023-04-25 | 2023-04-25 | Sterilization composition for preventing and treating gray mold |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116584485A (en) |
-
2023
- 2023-04-25 CN CN202310455929.2A patent/CN116584485A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN116584485A (en) | Sterilization composition for preventing and treating gray mold | |
| CN116267928A (en) | Sterilization composition containing bronopol | |
| CN104705324A (en) | Sterilizing composition containing zhongshengmycin and chitosan oligosaccharide | |
| CN112438259A (en) | Bactericide composition for preventing and treating white disease of foxtail millet | |
| CN114946869B (en) | Macadimia nut recession disease control agent for disease resistance breeding | |
| CN114391551B (en) | Novel fertilizer special for strawberries | |
| CN113994972B (en) | Composition for treating gummosis of nectarines | |
| CN115024332B (en) | Pesticide composition for preventing and treating pepper anthracnose | |
| CN114732013B (en) | Macadimia nut southern blight bactericide based on molecular disease resistance mechanism | |
| CN114847297B (en) | Pesticide composition for preventing and treating rice diseases | |
| CN115039772B (en) | Biopesticide composition for preventing and treating rust disease of cane shoots | |
| CN115413671B (en) | Sterilization composition for preventing and treating jackfruit rot | |
| CN115918665B (en) | Wheat seed dressing agent for preventing and treating wheat stem-based rot | |
| CN116019116B (en) | Pesticide powder and application method thereof | |
| CN116508753A (en) | Sterilization composition and bactericide for preventing and treating gray mold of eggplant and application of sterilization composition and bactericide | |
| CN114467950B (en) | Sterilization composition for preventing and treating taro epidemic disease | |
| CN115968880A (en) | Bactericidal composition for preventing and treating wheat powdery mildew | |
| CN116210700A (en) | Sterilization composition for preventing and treating strawberry anthracnose | |
| CN116458511A (en) | Composition containing polyoxin and difenoconazole | |
| CN105519548A (en) | Azoxystrobin and tricyclazole compounded suspension and preparation method thereof | |
| CN116267957A (en) | Biopesticide for preventing and treating pineapple heart rot | |
| CN115943962A (en) | Bactericidal composition for preventing and treating wheat sharp eyespot | |
| CN117099790A (en) | Sterilization composition for preventing and treating wheat stem-based rot | |
| CN115777726A (en) | Seed treatment composition containing paenibacillus polymyxa KN-03 and application thereof | |
| CN117044733A (en) | Biopesticide for preventing and treating anthracnose of avocado |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20240223 Address after: No. 5 Guangfa Road, Xiaoxi Town, Pinghe County, Zhangzhou City, Fujian Province, 363799 Applicant after: Fujian Wotu Mouzhen Agricultural Technology Co.,Ltd. Country or region after: China Address before: 541003 No. 53, Weirentou Village, Bantang Village, Ertang Township, Xiangshan District, Guilin City, Guangxi Applicant before: Guangxi Jisheng Biotechnology Group Co.,Ltd. Country or region before: China |