CN116530408A - Breeding method of highland barley variety with high dietary fiber and high beta-glucan waxy property - Google Patents
Breeding method of highland barley variety with high dietary fiber and high beta-glucan waxy property Download PDFInfo
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- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
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- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 235000021313 oleic acid Nutrition 0.000 description 1
- 229960002969 oleic acid Drugs 0.000 description 1
- 229940098695 palmitic acid Drugs 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
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- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4624—Hordeum vulgarus [barley]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Physiology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a biological detection technology, in particular to a breeding method of highland barley varieties with high dietary fibers and high beta-glucan waxy properties. The method comprises the following steps: s1 hybridization F1 generation: preparing hybrid combination by selecting sweet and reclaimed No. 5 of waxy highland barley variety as female parent (sweet and recognized wheat 2012001) and short highland barley (ZDM 07171) as male parent, preparing hybrid single spike 5 spikes, mixing and harvesting, then performing F1 generation south propagation and generation adding, mixing and sowing and mixing and harvesting; s2, south propagation F2 generation planting: planting the south-propagation F2 generation, and selecting long spikes and large black single spikes; s3, obtaining homozygous single plants: planting single spike, extracting microspores for microspore culture to obtain regenerated plants, transplanting the regenerated plants into a field to obtain homozygous single plants, and carrying out southern reproduction and seed reproduction on the obtained homozygous single plants; and obtaining a strain with high content of beta-glucan compared with the control reclamation 5 total dietary fiber through identification test and strain comparison test breeding. The cultivation time is short, and the highland barley variety with high dietary fiber and high beta-glucan waxy property is obtained.
Description
Technical Field
The invention relates to a biological detection technology, in particular to a breeding method of highland barley varieties with high dietary fibers and high beta-glucan waxy properties.
Background
Highland barley, also called naked barley, has the characteristics of high protein, high fiber, high vitamin, low fat and low sugar; is rich in 18 amino acids, and has rich functional components such as linoleic acid, palmitic acid, oleic acid and linolenic acid, gamma-aminobutyric acid, flavone, tocotrienol, tocopherol, resistant starch and the like; the beta-glucan content is 50 times that of wheat. With the pursuit of people on functional diet, the content of highland barley functional components becomes the main concern of food processors, the average content of beta-glucan in highland barley varieties in production is 5.03%, the average content of dietary fibers is 20.32%, amylopectin accounts for about 70% of total starch, the content of functional components is low, and amylopectin accounts for low total starch proportion, so that the highland barley addition amount in highland barley processed products is low, and the functional utility of highland barley products is further reduced. The traditional crossbreeding method has long breeding years, so that a new breeding method is applied to quality breeding work to accelerate the breeding of highland barley varieties special for processing high dietary fibers and high beta-glucan waxy.
Disclosure of Invention
The invention aims to provide a breeding method of highland barley varieties with high dietary fiber and high beta-glucan waxy quality, which aims to solve the problems of the prior art that the service time is long, and the highland barley varieties special for processing the high dietary fiber and the high beta-glucan waxy quality are insufficient.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a breeding method of highland barley varieties with high dietary fiber and high beta-glucan waxy quality comprises the following steps:
s1 hybridization F1 generation: preparing hybrid combination by selecting sweet and reclaimed No. 5 of waxy highland barley variety as female parent (sweet and recognized wheat 2012001) and short highland barley (ZDM 07171) as male parent, preparing hybrid single spike 5 spikes, mixing and harvesting, then performing F1 generation south propagation and generation adding, mixing and sowing and mixing and harvesting;
s2, south propagation F2 generation planting: planting the south-propagation F2 generation, and selecting long spikes and large black single spikes;
s3, obtaining homozygous single plants: planting single spike, extracting microspores for microspore culture to obtain regenerated plants, transplanting the regenerated plants into a field to obtain homozygous single plants, and carrying out southern reproduction and seed reproduction on the obtained homozygous single plants;
s4, identification test: selecting microspore culture strain for identification test, and introducing black highland barley with good property into strain comparison test by contrast and reclamation No. 5;
s5 strain comparison test: and breeding to obtain a strain with higher total dietary fiber content and higher beta-glucan content than the control reclamation 5.
Preferably, the specific steps for obtaining the regenerated plant in the step S3 are as follows: selecting a single spike to extract microspores, detecting microspore activity, and carrying out microspore dark culture on the microspores by using an N6 induction culture medium at 25 ℃ to obtain callus; transferring the callus to a 2/3MS differentiation culture medium, and culturing at 25 ℃ under illumination for 12 hours to obtain green seedlings; and when the green seedlings formed by differentiation grow to 1.5-2.0cm, transplanting the green seedlings to a 1/2MS rooting culture medium in time, and carrying out differentiation culture at the culture temperature and illumination to obtain regenerated plants.
Preferably, the specific step of transplanting the field in the step S3 to obtain the homozygous individual plant is as follows: and (3) opening a culture bottle cap overnight for hardening seedlings, then carrying out water culture for 2 weeks by using Hoagland nutrient solution, and transplanting the seedlings into a field to obtain a naturally doubled microspore culture stable homozygous single plant.
Preferably, in the S4 identification test, highland barley materials with the proportion of amylopectin accounting for more than 90 percent of total starch, the content of beta-glucan being more than 6 percent and the content of dietary fiber being more than 20 percent are selected to enter the identification test.
Preferably, in the S5 strain comparison test, 8 parts of black highland barley with the yield higher than that of the control sweet 5 No. 10%, good grain properties, the total starch proportion of more than 90%, the beta-glucan content of more than 6% and the dietary fiber content of more than 20% are selected to enter the strain comparison test.
Compared with the prior art, the invention has at least one of the following beneficial effects: the cultivation time is short, and the highland barley variety with high dietary fiber and high beta-glucan waxy property is obtained.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The following list a few examples:
the black highland barley with waxy property (the proportion of amylopectin is more than or equal to 95 percent of total starch), high beta-glucan (the content of beta-glucan is more than or equal to 5 percent) is selected as a female parent, the highland barley with high amylopectin proportion (the proportion of amylopectin is more than or equal to 80 percent) and high dietary fiber (the content of dietary fiber is more than or equal to 20 percent) is selected as a male parent, and the hybrid combination is prepared to obtain F1 generation seeds. F1 generation is planted into a single row, selfed (south propagation and generation adding), and mixed and collected in the whole row. F2 generation mixed sowing, sowing 10 rows of 1.5m rows, ensuring enough variation of F2 generation, and selecting long spike and large black single spike. The F2 generation is preferably selected as a single spike for microspore culture, the F2 generation single spike is planted into a single line of 1.5m, when the flag leaf distance is 0.5-2.0cm, the single spike is selected for microspore extraction, the microspore activity is detected, and the microspore dark culture is carried out by using an N6 induction culture medium at 25 ℃ to obtain callus; transferring the callus to a 2/3MS differentiation culture medium, and culturing at 25 ℃ under illumination for 12 hours to obtain green seedlings; when the green seedlings formed by differentiation grow to 1.5-2.0cm, transplanting the green seedlings to a 1/2MS rooting culture medium in time, and carrying out differentiation culture at the same time of culture temperature and illumination to obtain regenerated plants; and (3) opening a culture bottle cap overnight for hardening seedlings, then carrying out water culture by using Hoagland nutrient solution for about 2 weeks, and transplanting the seedlings into a field to obtain a naturally doubled microspore culture stable homozygous single plant. The single plant is planted into 1.5m plant lines, mature seeds are harvested, the proportion of amylopectin to total starch, the content of beta-glucan and dietary fiber are measured, black highland barley plants with the proportion of amylopectin to total starch being more than 90%, the content of beta-glucan being more than 6% and the content of dietary fiber being more than 20% are selected to go into an identification test, then yield, field lodging resistance and disease resistance evaluation and quality detection are carried out, and finally black waxy highland barley lines with good grain properties, high dietary fiber and high beta-glucan are selected to be used as special highland barley varieties for processing. The following is a detailed operation:
(1) In 2013, the content of amylopectin, beta-glucan and dietary fiber is measured on a sweet and cultivated No. 5 (sweet and cultivated wheat 2012001) of a self-bred waxy highland barley variety and 18 parts of highland barley introduced, and germplasm resources with high beta-glucan (the beta-glucan content is more than or equal to 5%), high amylopectin ratio (the proportion of the amylopectin to the total starch is more than or equal to 80%) and high dietary fiber (the dietary fiber content is more than or equal to 20%) are screened out to serve as parents.
(2) In 2014, waxy highland barley variety sweet 5 is selected as a female parent, high amylopectin ratio (amylopectin is more than or equal to 80% of total starch), high dietary fiber (dietary fiber content is more than or equal to 20%) resources short-burst highland barley (ZDM 07171) are selected as male parent to prepare hybrid combination, and hybrid single spike 5 is prepared, mixed harvest is carried out, and the number is 1408; f1 generation south propagation generation adding is carried out in Yunnan unit 10 months in the same year, mixed sowing is carried out, the line length is 1.5m, and the whole line is mixed and collected;
(3) In 2015, planting south-propagation F2 generation, mixedly sowing 10 rows with the row length of 1.5m, ensuring enough variation of F2 generation, and selecting long-spike and large-grain black single spike;
(4) 8 months in 2015, single spikes are planted, 1 spike is planted for 1 row (row length is 1.5 m), microspores are extracted for microspore culture in 12 months in 2016, regenerated plants are obtained, and homozygous single plants are obtained after transplanting in fields; and 10 months in 2016, 1 plant is planted for 1 row, the single row harvest number 1408B-1 to 1408B-50 is measured, the proportion of amylopectin to total starch, the content of beta-glucan and dietary fiber is measured, 23 parts of highland barley materials with the proportion of amylopectin to total starch being more than 90%, the content of beta-glucan being more than 6% and the content of dietary fiber being more than 20% are selected to enter an identification test.
(5) In 2017, identification tests are carried out on the 2016-year-preferred microspore culture strain, and 8 parts of black highland barley with the yield higher than that of the control sweet reclamation No. 5 by 10%, good grain properties, the total starch proportion of more than 90%, the beta-glucan content of more than 6% and the dietary fiber content of more than 20% are selected to enter the strain comparison test through yield, field lodging resistance and disease resistance evaluation and quality detection.
(6) In 2018-2019, strain comparison tests are carried out, the test area of a cell is 2 multiplied by 5m < 2 >, the sowing amount per mu is 35 ten thousand grains, the cell length is 5m, and 656 grains are sown in each row. And through comparison of the yield and the quality, the strain 1408B-7 with the yield increased by 51.0 percent, the crude starch content of 49.8 percent, the amylopectin content of 98.7 percent, the total dietary fiber content of 25.1 percent and the beta-glucan content of 8.40 percent is finally obtained through breeding.
(7) And in 2020-2021, taking part in Gansu province area test, and finally breeding to obtain strain 1408B-7 with yield increased by 25.9% compared with that of the control Gansu No. 5, and performing variety registration with tentative name Gansu glutinous No. 3.
Reference in the specification to a number of illustrative embodiments means that a particular structure or method described in connection with the embodiments is included in at least one embodiment described generally herein. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment. Further, when a structure is described in connection with any one embodiment, it is intended that such structure or method be implemented in connection with other embodiments to fall within the scope of the invention.
Claims (5)
1. A breeding method of highland barley varieties with high dietary fiber and high beta-glucan waxy quality is characterized by comprising the following steps: the method comprises the following steps:
s1 hybridization F1 generation: preparing hybridization combination by taking sweet highland barley variety sweet 5 (sweet wheat 2012001) as female parent and short highland barley (ZDM 07171) as male parent, preparing hybridization single spike 5 spikes, mixing and harvesting, then performing F1 generation south propagation and generation addition, mixing and harvesting after sowing;
s2, south propagation F2 generation planting: planting the south-propagation F2 generation, and selecting long spikes and large black single spikes;
s3, obtaining homozygous single plants: planting single spike, extracting microspores for microspore culture to obtain regenerated plants, transplanting the regenerated plants into a field to obtain homozygous single plants, and carrying out southern reproduction and seed reproduction on the obtained homozygous single plants;
s4, identification test: selecting microspore culture strain for identification test, and introducing black highland barley with good property into strain comparison test by contrast and reclamation No. 5;
s5 strain comparison test: and breeding to obtain a strain with higher total dietary fiber content and higher beta-glucan content than the control reclamation 5.
2. The method for breeding highland barley varieties with high dietary fiber and high beta-glucan waxy quality according to claim 1, which is characterized in that: the specific steps for obtaining the regenerated plants in the step S3 are as follows: selecting a single spike to extract microspores, detecting microspore activity, and carrying out microspore dark culture on the microspores by using an N6 induction culture medium at 25 ℃ to obtain callus; transferring the callus to a 2/3MS differentiation culture medium, and culturing at 25 ℃ under illumination for 12 hours to obtain green seedlings; and when the green seedlings formed by differentiation grow to 1.5-2.0cm, transplanting the green seedlings to a 1/2MS rooting culture medium in time, and carrying out differentiation culture at the culture temperature and illumination to obtain regenerated plants.
3. The method for breeding highland barley varieties with high dietary fiber and high beta-glucan waxy quality according to claim 2, which is characterized in that: the specific steps of transplanting the field in the step S3 to obtain the homozygous single plant are as follows: and (3) opening a culture bottle cap overnight for hardening seedlings, then carrying out water culture for 2 weeks by using Hoagland nutrient solution, and transplanting the seedlings into a field to obtain a naturally doubled microspore culture stable homozygous single plant.
4. The method for breeding highland barley varieties with high dietary fiber and high beta-glucan waxy quality according to claim 1, which is characterized in that: in the S4 identification test, highland barley materials with the proportion of amylopectin accounting for more than 90 percent of total starch, the content of beta-glucan more than 6 percent and the content of dietary fiber more than 20 percent are selected to enter the identification test.
5. The method for breeding highland barley varieties with high dietary fiber and high beta-glucan waxy quality according to claim 1, which is characterized in that: in the S5 strain comparison test, 8 parts of black highland barley with the yield higher than that of the control reclaimed No. 5 by 10%, good grain properties, the total starch proportion of more than 90%, the beta-glucan content of more than 6% and the dietary fiber content of more than 20% are selected to enter the strain comparison test.
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| CN202310550455.XA CN116530408A (en) | 2023-05-16 | 2023-05-16 | Breeding method of highland barley variety with high dietary fiber and high beta-glucan waxy property |
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Citations (5)
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| CA2887960A1 (en) * | 2014-04-10 | 2015-10-10 | The United States Of America, As Represented By The Secretary Of Agricture | Barley mutant lines having grain with ultra-high beta glucan content |
| CN106234204A (en) * | 2015-10-23 | 2016-12-21 | 中国科学院成都生物研究所 | A kind of breeding method of high beta glucan content Semen avenae nudae |
| CN112273230A (en) * | 2020-10-29 | 2021-01-29 | 上海市农业科学院 | Method capable of remarkably increasing callus yield of Simala 22 microspores of highland barley variety |
| CN114303953A (en) * | 2022-01-06 | 2022-04-12 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | High-efficiency breeding method of high-yield highland barley |
| CN114467742A (en) * | 2022-03-01 | 2022-05-13 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | Breeding method of black-grain highland barley |
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| CA2887960A1 (en) * | 2014-04-10 | 2015-10-10 | The United States Of America, As Represented By The Secretary Of Agricture | Barley mutant lines having grain with ultra-high beta glucan content |
| CN106234204A (en) * | 2015-10-23 | 2016-12-21 | 中国科学院成都生物研究所 | A kind of breeding method of high beta glucan content Semen avenae nudae |
| CN112273230A (en) * | 2020-10-29 | 2021-01-29 | 上海市农业科学院 | Method capable of remarkably increasing callus yield of Simala 22 microspores of highland barley variety |
| CN114303953A (en) * | 2022-01-06 | 2022-04-12 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | High-efficiency breeding method of high-yield highland barley |
| CN114467742A (en) * | 2022-03-01 | 2022-05-13 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | Breeding method of black-grain highland barley |
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| 科创中国: ""糯青稞新品种甘垦糯3号"", Retrieved from the Internet <URL:https://www.kczg.org.cn/fruit/detail?id=566437> * |
| 陈晓静;颜伟;陈和;陈健;沈会权;周汝琴;陶红;乔海龙;臧慧;: "食用糯性裸大麦研究进展", 大麦与谷类科学, no. 03, pages 17 - 19 * |
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