CN106797884B - A kind of selection of high oleic acid Silk channel injection - Google Patents
A kind of selection of high oleic acid Silk channel injection Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The present invention relates to peanut breeding technical fields, and in particular to a kind of selection of high oleic acid Silk channel injection.Selection of the invention, comprising the following steps: (1) again by obtaining the high oleic acid strain 8-153 that high oleic acid content is not less than 80% by generation selfing, directed screening after being hybridized female parent 1 with male parent 1；(2) again by obtaining totally 11 plants of high oleic acid strain that high oleic acid content is not less than 80% by generation selfing, directed screening after high oleic acid strain 8-153 being hybridized with another male parent 2；(3) again by obtaining high oleic acid peanut new germ plasm by generation selfing, directed screening after 11 plant height oleic acid peanut strains being hybridized with another male parent 3 respectively；The peanut new germ plasm that selects using this breeding technique while having the characteristics that oleic acid content is high, linoleic acid content is low, high yield, kernel percent are higher, resistance to bacterial wilt, disease-resistanting leaf spot, Resistant Gene To Rust.
Description
[technical field]
The present invention relates to peanut breeding technical fields, and in particular to a kind of selection of high oleic acid Silk channel injection.
[background technique]
Peanut is the main oil crops in China and industrial crops, and main fatty acid is oleic acid and linoleic acid in peanut,
In common peanut, the content of oleic acid is about 45%, and linoleic content is about 35%.Wherein, linoleic acid is to cause grease corrupt
Rotten basic reason, and oleic acid can reduce blood of human body total cholesterol and harmful low density lipoprotein cholesterol, but to having
The high-density lipoprotein cholesterol of benefit does not influence then, is of great significance in terms of reducing the heart disease risks such as coronary heart disease；Separately
Outside, oleic acid be not easy to be oxidized, product shelf-stable, it may have important industrial economy value.The vegetable oil of high oleic acid content is in city
It is widely appreciated by consumers on, is such as known as the olive oil of " liquid golden " good reputation, oleic acid content is up to 80% or more, and there are also camellias
Oil, oleic acid content is also very high, and nutrition circle usually calls oleic acid " safe fats acid ", and the content of oleic acid is to evaluate edible oil product
The one of the important signs that of matter.In recent ten years, improving the oleic acid content of peanut, reducing the linoleic acid content of peanut is always peanut
The research hotspot of breeder's improvement peanut quality.Further, since peanut is that the most important industrial crops in China and oil plant are made
One of object, high yield are always the main target of peanut breeding.
Peanut high-oleic acid breeding research is just carried out early in the 1980s in the U.S..1987, Norden etc. was from right
Oleic acid content 80% or so high oleic acid germplasm " F435 " (Norden et is had found in the fatty acid analysis of peanut breeding material
al,1987).With " F435 " for high oleic acid parent, Florida, US agricultural experiment station is existed by sexual hybridization, backcrossing means
Nineteen ninety-five has been bred as high oleic acid peanut varieties " Sunoleic95R " (Gorbet and Knauft 1997) earliest.Subsequent
In 10 years or so times, it is new that U.S.'s breeding units or company have been bred as more than 10 a high oleic acid peanuts such as " SunOliec97 " in succession again
Kind (Chen Jing, 2011).After domestic peanut high-oleic acid breeding research probably starts from 2000, including Guangxi Academy of Agricultural Sciences's economy
Some research institutions, the country including crop research institute introduce high oleic acid Silk channel injection from the U.S. in succession and carry out research on utilization.2003
Year Yu Shanlin etc. obtains a collection of high oleic acid mutant using method of radiating, is that parent educated in 2009 using " SP1098 " therein
At high oleic acid new varieties " flower educates 32 " and pass through Shandong Province's crop varietal approval committee.Kaifeng academy of agricultural sciences in
Incubation high oleic acid kinds " opening agriculture H03-3 " in 2006 simultaneously pass through Anhui Province's cultivar identification (Chen Jing, 2011).Cut-off 2015, I
State be bred as in succession 18 high oleic acid new peanut varieties (Wang Chuantang, 2015；Wang Chuantang, 2016).But peanut in south China
It is still reported so far without the incubation of high oleic acid peanut varieties in producing region.It is local that southern ecoclimatic condition is mainly suitable for plantation
The Spanish type kind of breeding, it is inclined in locally plantation common manifestation result quoted from the peanut varieties of northern China or foreign countries
Less, yield is unstable, later period early ageing, disease resistance difference etc., it is difficult in the upper directly application of production.Guangxi Academy of Agricultural Sciences's warp in 2001
Crop research institute of helping introduces high oleic acid kind " Sunoleic95R " from the U.S., it is found after planting experimentally, and there is also above-mentioned similar
Problem, thus determine breeding improvement is localized to it, by with mixing breed in region, select be suitble in south China
The Pearl Type high oleic acid peanut new germ plasm of peanut producing region plantation.
[summary of the invention]
Goal of the invention of the invention is: for high oleic acid kind " Sunoleic95R " production present in locally planting experimentally
Measure low and other character defects, select the plantation of suitable one's respective area and with high oleic acid, low linoleic acid and high yield, kernel percent compared with
Height, the strong Pearl Type high oleic acid peanut new germ plasm of field Resistance to bacterial wilt, leaf spot resistance, rust resistance.
To achieve the goals above, The technical solution adopted by the invention is as follows:
A kind of selection of high oleic acid Silk channel injection, the described method comprises the following steps:
(1) female parent 1 is hybridized with male parent 1, obtains first generation filial generation F1, the seed of F1 generation is rejected into the mixed receipts of pseudostationary
After be selfed to obtain F2 generation, will F2 generation seed be selfed after obtain F3 generation, will F3 generation seed be selfed after obtain F4 for seed；
Oleic acid content analysis is carried out for kernel to the F4, and filters out the Kernel of Peanut that oleic acid content is not less than 80%, obtains high oil
Sour peanut strain 8-153；
(2) the high oleic acid peanut strain 8-153 of step (1) is hybridized to obtain with the male parent 2 of another high-yield disease resisting another
One filial generation F ' 1 is selfed to obtain F ' 2 generation seed, carries out oil to the F ' 2 generation kernel after 1 generation of F ' is rejected the mixed receipts of pseudostationary
Acid content analysis, and filter out totally 11 plants of high oleic acid peanut strain that oleic acid content is not less than 80%；The 11 plant height oleic acid flower
Raw strain is respectively as follows: high oleic acid strain 14, high oleic acid strain 16, high oleic acid strain 107, high oleic acid strain 109, high oleic acid strain
150, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain 425, high oleic acid strain 586, high oleic acid strain 902 and height
Oleic acid strain 1083；
(3) 11 plant height oleic acid peanut strains of step (2) are hybridized to obtain with the male parent 3 of another high-yield disease resisting respectively
Filial generation F " 1, by F " seed in 1 generation is selfed to obtain F after rejecting the mixed receipts of pseudostationary " 2 generations, by F, " in 2 generations, were selfed to obtain
In F " 2 generations and F " 3 generations, are carried out mixed planting and obtain 4 generation of F " by 3 generation of F ", are rejected F " 4 generations were selfed to obtain F " 5 after pseudostationary
Generation；F " 5 generations were selfed to obtain F " 6 generations；Oleic acid content analysis is carried out to the F " 6 generation kernel and carries out strain comparison, is selected
Elite plant strain enters identification garden, the yield and quality qualification test of 2 years multiple spots is then carried out, to obtain high oleic acid peanut novel species
Matter.
Further, described maternal 1 is Shanyou 162, and the male parent 1 is Sunleic95R, and the male parent 2 is Guangdong oil 13.
Further, the male parent 3 is profound oil 75.
Further, the male parent 3 is Guangdong oil 45.
Further, oleic acid content analysis is carried out to F4 generation and/or F ' 2 generation kernel in step (1) and/or step (2)
Method is high resolution gas chromatography method.
Further, the method for carrying out oleic acid content analysis to F " 6 generation kernel in step (3) is near-infrared analysis method.
Above-mentioned parent material source:
" Sunleic95R " is the height oil introduced in 2001 from the U.S. by industrial crops research institute, Guangxi Academy of Agricultural Sciences
Sour kind (oleic acid content 80% or so) (Gorbet and Knauft 1997)；
" Shanyou 162 " is to be bred as (Zheng Yixiong, 2005) by Shantou City, Guangdong Province Institute of agricultural sciences；
" Guangdong oil 13 " and " Guangdong oil 45 " be by Crop Institute, Guangdong Academy of Agricultural Sciences be bred as (Hong Yanbin, 2011；Lee is few
Hero, 2010)；
" profound oil 75 " is to be bred as (Wang Yun, 2011) by Zhanjiang, Guangdong Province Institute of agricultural sciences.
The beneficial effects of the present invention are:
1, the present invention is carried out using the kind " Sunleic95R " of local high yield, the peanut varieties of high disease resistance and introduction
Sexual hybridization, in hybrid process, other than the good germplasm materials of M8003 line, also by 2 sexual hybridizations to filial generation into
Row improvement, further improves the genetic recombination ability of peanut, realizes the complementation of merit, effectively increases and realizes breeding mesh
Target success rate further shortens breeding time, meanwhile, so that the new germ plasm selected is suitable for south China ecology gas
Time condition, reaches that oleic acid content is high, linoleic acid content is low and high yield, and kernel percent is higher, and field Resistance to bacterial wilt, leaf spot are anti-
Property, rust resistance is strong and the advantage of suitable one's respective area plantation.
2, in breeding process, the present invention analyzes peanut high-oil acid content using nondestructive NIR technology,
The technical operation is simple, accuracy is high, finding speed is fast, is able to satisfy the requirement of breeding.
[Detailed description of the invention]
Fig. 1 is the family tree of breeding method of the present invention.
[specific embodiment]
Below with reference to embodiment, the invention will be further described.
Embodiment 1:
A kind of selection of high oleic acid Silk channel injection as shown in Figure 1, method includes the following steps:
In (1) 2003 year spring, by maternal 1 (Shanyou 162: having the characteristics that high-yield disease resisting) and male parent 1, (SunOliec95R is
Applicant passes through the high oleic acid peanut varieties that 948 projects are introduced from the U.S., analyzes its oil through oil analysis test center, the Ministry of Agriculture
Acid content 64.14%, linoleic acid 15.86%, oil/Asia are hybridized than 4.04), obtain first generation filial generation F1, and the spring in 2004 will
The seed of F1 generation is selfed to obtain F2 generation after rejecting the mixed receipts of pseudostationary, and F2 generation all single plants in harvest individually harvest；2004
Autumn in year will obtain F3 generation after the seed selfing in F2 generation, and the spring in 2005 will obtain F4 for seed after the seed selfing in F3 generation；To described
F4 carries out oleic acid content analysis (analysis method are as follows: high resolution gas chromatography method) for kernel, and filters out oleic acid content and be not less than
80% Kernel of Peanut, and acquisition high oleic acid peanut strain 8-153 (compared with former high oleic acid parent " Sunleic95R ", " 8-153 "
Plant shape straightened it is vertical, pod also become larger become it is more, but its yield compared with local main breed also big gap)；
(2) 2006 year autumn, by (the Guangdong oil of male parent 2 of the high oleic acid peanut strain 8-153 of step (1) and another high-yield disease resisting
13: having the characteristics that high-yield disease resisting) hybridized to obtain another filial generation F ' spring in 1,2007,1 generation of F ' is rejected into the mixed receipts of pseudostationary
After be selfed to obtain F ' 2 generation seed, F ' 2 withholds all single plants when obtaining and individually harvests；Oleic acid is carried out to the F ' 2 generation kernel to contain
Amount analysis (analysis method are as follows: high resolution gas chromatography method), and the Kernel of Peanut that oleic acid content is not less than 80% is filtered out, obtain 11
Plant height oleic acid peanut strain；The 11 plant height oleic acid peanut strain is respectively as follows: high oleic acid strain 14, high oleic acid strain 16, high oil
Sour strain 107, high oleic acid strain 109, high oleic acid strain 150, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain
425, high oleic acid strain 586, high oleic acid strain 902 and high oleic acid strain 1083 are (in 2008-2009, to the height of above-mentioned acquisition
Oleic acid peanut strain carries out strain and variety test of lines, finds the yield traits of these high oleic acid peanut strains or not enough manages
Think, pod is few and universal shorter)；
(3) 2010 spring, (the present embodiment was only explanation of giving one example, above-mentioned by the high oleic acid peanut strain 425 of step (2)
11 plant height oleic acid peanut strains: it is high oleic acid strain 14, high oleic acid strain 16, high oleic acid strain 107, high oleic acid strain 109, high
Oleic acid strain 150, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain 425, high oleic acid strain 586, high oleic acid strain
It is that 902 and high oleic acid strain 1083 are able to satisfy requirement of the invention) (profound oil 75: there is height with the male parent 3 of another high-yield disease resisting
Produce disease-resistant feature) filial generation F " 1 is obtained, by F " seed in 1 generation is selfed to obtain F after rejecting the mixed receipts of pseudostationary " 2 generations, by F " 2
In generation, is selfed to obtain 3 generation of F ", and F " 2 generations and F " 3 generations are carried out mixed planting and obtain 4 generation of F ", is rejected 4 generation of F " after pseudostationary
It is selfed to obtain 5 generation of F "；In F " 5 generations were selfed to obtain F " 6 generations, (in the Breeding Process in F " 1 generation-F " 6 generations, eliminate result
Then the single plant relatively fewer, economical character is relatively poor is planted again)；Oleic acid content point is carried out to the F " 6 generation kernel
Analysis (analysis method are as follows: near-infrared analysis method) simultaneously carries out strain comparison, selects elite plant strain to enter identification garden, then carries out 2 years
The yield and quality qualification test of multiple spot obtains high oleic acid peanut new germ plasm 1 (12 autumn strain/5-3).
Embodiment 2
A kind of selection of high oleic acid Silk channel injection as shown in Figure 1, method includes the following steps:
In (1) 2003 year spring, by maternal 1 (Shanyou 162: having the characteristics that high-yield disease resisting) and male parent 1, (SunOliec95R is
Applicant passes through the high oleic acid peanut varieties that 948 projects are introduced from the U.S., analyzes its oil through oil analysis test center, the Ministry of Agriculture
Acid content 64.14%, linoleic acid 15.86%, oil/Asia are hybridized than 4.04), obtain first generation filial generation F1, and the spring in 2004 will
The seed of F1 generation is selfed to obtain F2 generation after rejecting the mixed receipts of pseudostationary, and F2 generation all single plants in harvest individually harvest；2004
Autumn in year will obtain F3 generation after the seed selfing in F2 generation, and the spring in 2005 will obtain F4 for seed after the seed selfing in F3 generation；To described
F4 carries out oleic acid content analysis (analysis method are as follows: high resolution gas chromatography method) for kernel, and filters out oleic acid content and be not less than
80% Kernel of Peanut, and acquisition high oleic acid peanut strain 8-153 (compared with former high oleic acid parent " Sunleic95R ", " 8-153 "
Plant shape straightened vertical, pod also become larger become it is more, but its yield compared with local main breed also big gap)；
(2) 2006 year autumn, by (the Guangdong oil of male parent 2 of the high oleic acid peanut strain 8-153 of step (1) and another high-yield disease resisting
13: having the characteristics that high-yield disease resisting) hybridized to obtain another filial generation F ' spring in 1,2007,1 generation of F ' is rejected into the mixed receipts of pseudostationary
After be selfed to obtain F ' 2 generation seed, F ' 2 withholds all single plants when obtaining and individually harvests；Oleic acid is carried out to the F ' 2 generation kernel to contain
Amount analysis (analysis method are as follows: high resolution gas chromatography method), and the Kernel of Peanut that oleic acid content is not less than 80% is filtered out, obtain 11
Plant height oleic acid peanut strain；The 11 plant height oleic acid peanut strain is respectively as follows: high oleic acid strain 14, high oleic acid strain 16, high oil
Sour strain 107, high oleic acid strain 109, high oleic acid strain 150, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain
425, high oleic acid strain 586, high oleic acid strain 902 and high oleic acid strain 1083 are (in 2008-2009, to the height of above-mentioned acquisition
Oleic acid peanut strain carries out strain and variety test of lines, finds the yield traits of these high oleic acid peanut strains or not enough manages
Think, pod is few and universal shorter)；
(3) 2010 spring, (the present embodiment was only explanation of giving one example, above-mentioned by the high oleic acid peanut strain 16 of step (2)
11 plant height oleic acid peanut strains: it is high oleic acid strain 14, high oleic acid strain 16, high oleic acid strain 107, high oleic acid strain 109, high
Oleic acid strain 150, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain 425, high oleic acid strain 586, high oleic acid strain
It is that 902 and high oleic acid strain 1083 are able to satisfy requirement of the invention) and (the Guangdong oil 45: the spy with high-yield disease resisting of another male parent 3
Point) filial generation F " 1 is obtained, by F " seed in 1 generation is selfed to obtain F after rejecting the mixed receipts of pseudostationary " 2 generations, by F, " progress in 2 generations is certainly
Friendship obtains 3 generation of F ", and F " 2 generations and F " 3 generations are carried out mixed planting and obtain 4 generation of F ", be selfed by 4 generation of F " after rejecting pseudostationary
" 5 generations to F；F " 5 generations were selfed to obtain F " 6 generations (in the Breeding Process in F " 1 generation-F " 6 generations, eliminate result it is relatively fewer,
Then the relatively poor single plant of economical character is planted again)；Oleic acid content analysis (analysis side is carried out to the F " 6 generation kernel
Method are as follows: near-infrared analysis method) and strain comparison is carried out, it selects elite plant strain to enter identification garden, then carries out the yield of 2 years multiple spots
Quality identification test, obtains high oleic acid peanut new germ plasm 2 (12 autumn strain/1-3) and new germ plasm 3 (12 autumn strain/1-8).
Above-mentioned new germ plasm 2 has following characteristic after measured:
The time of infertility about 125 days or so, plant type was uprightly compact, and growth potential is strong, in blade, leaf color green.Each economical character
And economic characters species test average value are as follows: the high 60.8cm of stem, long branch 62.1cm, total branch amount 6.5 are 4.7, fruitful branch, single
Strain number of results 13.3, plumpness 87.17%, double a kind of fruit, such as apple, pear, etc. rates 79.3%, all kinds of fruits weight 186.4g, hundred benevolence weight 63.6g, kilogram fruit number
668, kernel percent 60.22%.
Plant experimentally comparative test:
Comparative test 1:
Test year and place: 2013；Nanning Wuming County.
Test specimen: the new germ plasm 2 (12 obtained in the new germ plasm 1 (12 autumn strain/5-3) that is obtained in embodiment 1, embodiment 2
Autumn strain/1-3) and new germ plasm 3 (12 autumn strain/1-8).
Control sample: Shanyou 523.
Cultural technique: selection is sunny, and soil layer is deep, is test block rich in organic matter and calcareous sandy loam, presses
It is planted experimentally according to unified standard and field management.Plantation is planted using " playing furrow duplicate rows double grains " mode.Specification: furrow are 80 centimetres wide
(33 centimetres of row ditch, furrow face are 47 centimeters wide), furrow are 13-16 centimetres high, every two row of drill seeding kind, two 23-27 centimetres of line-spacings, hill spacing 13-
16 centimetres, every bunch planting kind 2.Sowing each sample Kernel of Peanut is equal in weight per acre.
Field management: it is carried out by unified standard, heavy dressing base manure, in due course top dressing.Mu basal dressing: phosphate fertilizer (45 kilograms)+peanut
Bran (20 kilograms)+pigs and cattle excrement (130 kilograms)+plant ash (75) kilogram+farmyard manure (4000 kilograms), after mixing stack retting is decomposed,
65% spreads fertilizer over the fields and makees base fertilizer, and 35% makees lid seed manure.After planting timely herbicide spraying.When 3-4 piece true leaf, applied according to seedling growing way is suitable
6.5 kilograms of urea.Needle ridging is met after blooming 15 days, imposes calcium, potash fertilizer (25 kilograms of 25 kilograms+plant ash of lime).
Interpretation of result: contained by the oleic acid in Ministry of Agriculture's oil plant and quality of item supervision and inspection center analysis test seed benevolence
Amount, counts and calculates per mu yield, concrete outcome see the table below 1.
1 test result statistical form of table
As can be seen that the oleic acid content of new germ plasm 1, new germ plasm 2 and new germ plasm 3 is all apparently higher than pair from upper 1 result of table
According to kind, the Pod Yield of new germ plasm 2 is more slightly higher than control kind, and new germ plasm 1, the Pod Yield of new germ plasm 3 are more slightly lower than control kind.
In order to further carry out stability and universality test to new germ plasm 2, the present invention has carried out further examination
Test research:
Comparative test 2:
Test year: 2014 and 2015.
Test site: Guangxi region: including Nanning, Guilin, the North Sea, He Prefecture, Liuzhou, Pingnan County and other places；National south
Square piece: including Fujian Nanan, gaozhou,guangdong and other places.
Test specimen: new germ plasm 2 obtained in embodiment 2 (12 autumn strain/1-3).
Control sample: Shanyou 523 and sweet osmanthus 21.
Cultural technique: selection is sunny, and soil layer is deep, is test block rich in organic matter and calcareous sandy loam, presses
It is planted experimentally according to unified standard and field management.Plantation is planted using " playing furrow duplicate rows double grains " mode.Specification: furrow are 80 centimetres wide
(33 centimetres of row ditch, furrow face are 47 centimeters wide), furrow are 13-16 centimetres high, every two row of drill seeding kind, two 23-27 centimetres of line-spacings, hill spacing 13-
16 centimetres, every bunch planting kind 2.Sowing each sample Kernel of Peanut is equal in weight per acre.
Field management: it is carried out by unified standard, heavy dressing base manure, in due course top dressing.Mu basal dressing: phosphate fertilizer (45 kilograms)+peanut
Bran (20 kilograms)+pigs and cattle excrement (130 kilograms)+plant ash (75) kilogram+farmyard manure (4000 kilograms), after mixing stack retting is decomposed,
65% spreads fertilizer over the fields and makees base fertilizer, and 35% makees lid seed manure.After planting timely herbicide spraying.When 3-4 piece true leaf, applied according to seedling growing way is suitable
6.5 kilograms of urea.Needle ridging is met after blooming 15 days, imposes calcium, potash fertilizer (25 kilograms of 25 kilograms+plant ash of lime).
Interpretation of result:
(1) it is averaged crude fat by the seed benevolence of Ministry of Agriculture's oil plant and quality of item supervision and inspection center measurement new germ plasm 2
Content；And it measures the average oleic acid content in fatty acid constituents, average linoleic acid content and the sub- ratio of average oil, concrete outcome and sees below
Table 2.
(2) according to national southern piece and Guangxi peanut variety regional Disease Resistance Identification, the sick nursery of new germ plasm 2 and artificial
Inoculated identification disease resistance, concrete outcome see the table below 3.
(3) average pod production of new germ plasm 2 is counted and calculates, concrete outcome see the table below 4.
Average crude fat, oleic acid, linoleic acid content and oil/Asia ratio of 2 new germ plasm 2 of table
By upper table 2 it is found that the average oleic acid content of new germ plasm 2 is apparently higher than control kind；Average linoleic acid content is obviously low
In control kind；Average oil is sub- than being apparently higher than control kind.
The disease resistance of 3 new germ plasm 2 of table tests table
By upper table 3 it is found that Resistance to bacterial wilt, leaf spot resistance and the rust resistance of new germ plasm 2 are superior to control kind.
The average pod production statistical form of 4 new germ plasm 2 of table
By upper table 4 it is found that the average pod production of new germ plasm 2 is suitable with control kind.
In conclusion carrying out peanut breeding using method of the invention, high oleic acid kind can effectively improve
" Sunoleic95R " the defects of existing low output, disease-resistant difference, can select suitable one's respective area plantation when Guangxi is planted
And with high oleic acid, low linoleic acid and high yield, kernel percent is higher, and field Resistance to bacterial wilt, leaf spot resistance, rust resistance are equal
Strong Pearl Type high oleic acid peanut new germ plasm.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim.
Claims (4)
1. it is a kind of improve Suno leic95R high oleic acid Silk channel injection selection, which is characterized in that the method includes with
Lower step:
(1) female parent 1 is hybridized with male parent 1, obtains first generation filial generation F1, it is laggard that the seed of F1 generation is rejected the mixed receipts of pseudostationary
Row selfing obtains F2 generation, obtains F3 generation after the seed in F2 generation is selfed, and obtains F4 for seed after the seed in F3 generation is selfed；To institute
It states F4 and carries out oleic acid content analysis for kernel, and filter out the Kernel of Peanut that oleic acid content is not less than 80%, obtain high oleic acid flower
Raw strain 8-153；
(2) the high oleic acid peanut strain 8-153 of step (1) is hybridized to obtain another son with the male parent 2 of another high-yield disease resisting
For F ' 1, it is selfed to obtain F ' 2 generation seed after 1 generation of F ' is rejected the mixed receipts of pseudostationary, oleic acid is carried out to the F ' 2 generation kernel and is contained
Amount analysis, and filter out totally 11 plants of high oleic acid peanut strain that oleic acid content is not less than 80%；The 11 plant height oleic acid peanut strain
System be respectively as follows: high oleic acid strain 14, high oleic acid strain 16, high oleic acid strain 107, high oleic acid strain 109, high oleic acid strain 150,
High oleic acid strain 212, high oleic acid strain 421, high oleic acid strain 425, high oleic acid strain 586, high oleic acid strain 902 and high oleic acid
Strain 1083；
(3) by 11 plant height oleic acid peanut strains of step (2) respectively hybridized to obtain with the male parent 3 of another high-yield disease resisting it is another
Filial generation F " 1, by F " seed in 1 generation is selfed to obtain F after rejecting the mixed receipts of pseudostationary " 2 generations, by F, " in 2 generations, were selfed to obtain
In F " 2 generations and F " 3 generations, are carried out mixed planting and obtain 4 generation of F " by 3 generation of F ", are rejected F " 4 generations were selfed to obtain F " 5 after pseudostationary
Generation；F " 5 generations were selfed to obtain F " 6 generations；Oleic acid content analysis is carried out to the F " 6 generation kernel and carries out strain comparison, is selected
Elite plant strain enters identification garden, the yield and quality qualification test of 2 years multiple spots is then carried out, to obtain high oleic acid peanut novel species
Matter；
Described maternal 1 is Shanyou 162, and the male parent 1 is Suno leic95R, and the male parent 2 is Guangdong oil 13；
The male parent 3 is Guangdong oil 45.
2. a kind of selection for improveing Suno leic95R high oleic acid Silk channel injection according to claim 1, feature
It is, the male parent 3 is profound oil 75.
3. a kind of selection for improveing Suno leic95R high oleic acid Silk channel injection according to claim 1, feature
It is, the method for carrying out oleic acid content analysis to F4 generation and/or F ' 2 generation kernel in step (1) and/or step (2) is efficient air
Phase chromatography.
4. a kind of selection for improveing Suno leic95R high oleic acid Silk channel injection according to claim 1, feature
It is, the method for carrying out oleic acid content analysis to F " 6 generation kernel in step (3) is near-infrared analysis method.
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| CN110089419A (en) * | 2019-05-21 | 2019-08-06 | 广东省农业科学院作物研究所 | A kind of breeding method of high-oleic acid high-yield peanut |
| CN110367116B (en) * | 2019-06-17 | 2022-03-11 | 广东省农业科学院作物研究所 | High-oleic acid peanut hybridization breeding method |
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