CN116491432A - Cerebral palsy model incubator - Google Patents
Cerebral palsy model incubator Download PDFInfo
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- CN116491432A CN116491432A CN202310764898.9A CN202310764898A CN116491432A CN 116491432 A CN116491432 A CN 116491432A CN 202310764898 A CN202310764898 A CN 202310764898A CN 116491432 A CN116491432 A CN 116491432A
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- 206010008129 cerebral palsy Diseases 0.000 title claims abstract description 72
- 239000007789 gas Substances 0.000 claims abstract description 41
- 238000010438 heat treatment Methods 0.000 claims abstract description 34
- 238000007789 sealing Methods 0.000 claims abstract description 26
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 15
- 239000001301 oxygen Substances 0.000 claims abstract description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 6
- 230000004308 accommodation Effects 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 230000004323 axial length Effects 0.000 claims description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 3
- 239000001569 carbon dioxide Substances 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 abstract description 13
- 206010021143 Hypoxia Diseases 0.000 abstract description 11
- 230000007954 hypoxia Effects 0.000 abstract description 10
- 238000000465 moulding Methods 0.000 abstract description 6
- 238000004891 communication Methods 0.000 abstract description 4
- 238000010171 animal model Methods 0.000 description 13
- 238000012258 culturing Methods 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 238000000034 method Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
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- 238000006213 oxygenation reaction Methods 0.000 description 3
- 241000700159 Rattus Species 0.000 description 2
- RKTYLMNFRDHKIL-UHFFFAOYSA-N copper;5,10,15,20-tetraphenylporphyrin-22,24-diide Chemical compound [Cu+2].C1=CC(C(=C2C=CC([N-]2)=C(C=2C=CC=CC=2)C=2C=CC(N=2)=C(C=2C=CC=CC=2)C2=CC=C3[N-]2)C=2C=CC=CC=2)=NC1=C3C1=CC=CC=C1 RKTYLMNFRDHKIL-UHFFFAOYSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
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- 238000012795 verification Methods 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 101710138657 Neurotoxin Proteins 0.000 description 1
- 208000030886 Traumatic Brain injury Diseases 0.000 description 1
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- 210000004556 brain Anatomy 0.000 description 1
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- 208000029028 brain injury Diseases 0.000 description 1
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- 239000002581 neurotoxin Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/0047—Air-conditioning, e.g. ventilation, of animal housings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- General Health & Medical Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application discloses a cerebral palsy model incubator, which comprises a case body, wherein a culture chamber is formed in the case body, and the culture chamber is filled with low-oxygen mixed gas; one or more push-pull channels are formed in the side wall of the culture chamber, a culture bin is arranged in each push-pull channel, each culture bin comprises a model accommodating groove, end covers are respectively arranged at two ends of each model accommodating groove, sealing elements are arranged on the end covers, one end of each culture bin extends into the culture chamber along the push-pull channel, and the other end of each culture bin seals the push-pull channel through the sealing element on the other end of each culture bin; the model accommodating groove is in gas communication with the culture chamber through the mesh holes on the mesh hole cover arranged on the model accommodating groove, so that the frequent opening and closing of the door does not influence the gas environment in each culture bin, the problem that the animal is oxygenated in certain time periods of continuous constant hypoxia after the frequent opening and closing of the door of the three-gas incubator is used in the prior art is solved, and the success rate of molding the cerebral palsy model is improved; in addition, the bottom of the model accommodating groove is provided with a heating pad, so that the molding temperature can be ensured to be constant.
Description
Technical Field
The application relates to the technical field of animal cerebral palsy model modeling for medical research, in particular to a cerebral palsy model incubator for animal cerebral palsy model modeling.
Background
Cerebral palsy, abbreviated as cerebral palsy, is a non-progressive brain injury syndrome caused by various reasons from prenatal to postnatal brain development stage, and is a main disease causing limb disability of children. The WHO reports that 5 per mill of neonates generate cerebral palsy every year worldwide, and the morbidity in the part of China is 0.5 per mill to 2 per mill. At present, the pathogenesis of cerebral palsy is not clear. There is no effective therapeutic means for cerebral palsy patients clinically.
In order to clarify the pathogenesis of the cerebral palsy and effectively prevent and treat the cerebral palsy, a large number of cerebral palsy animal models are manufactured at home and abroad. The mouse is very similar to human beings due to nerve anatomy, has proper size and small brain volume, is convenient for observing pathological changes and biochemical changes of brain tissues, is economical, and is widely applied to research of cerebral palsy models.
The existing cerebral palsy model preparation method mainly comprises five types of ischemic and anoxic types, infectious types, neurotoxin types, brain trauma types and multi-method combination types. The ischemia and hypoxia model is a relatively common cerebral palsy animal model, and three modeling modes are mainly adopted: unilateral carotid ligation, bilateral carotid ligation, uterine artery ligation.
The most suitable environment for modeling cerebral palsy model is to place animals in an anoxic device with constant temperature of 37 ℃ and continuously introduce 6 to 10 percent of O into the anoxic device 2 And 92% N 2 The molding is performed, so the key factors for molding are constant temperature and oxygen concentration.
There is no special purpose on the marketIn the cerebral palsy model modeling culture device, a three-gas incubator for culturing microorganisms or cells is mainly used for simulating an anoxic environment, culture conditions suitable for cerebral palsy model culture are manufactured by using the three-gas incubator, and specifically, two gases are introduced into the three-gas incubator: 8% O 2 And 92% N 2 The internal temperature was set at 37 ℃. The laboratory three-gas incubator is expensive, but has large capacity, and can be used for placing a plurality of manufactured models, so that a plurality of three-gas incubators are purchased for culturing cerebral palsy models in a common laboratory.
The inventor finds in practice that the manufactured cerebral palsy model can be put into a three-gas incubator, so that the three-gas incubator can be frequently opened and closed according to the placing requirement at different time intervals, the gas content of the three-gas incubator at the time can be influenced by opening and closing the door each time, the culture environment of the cerebral palsy model at the time is destroyed, the gas content in the incubator can be automatically regulated by the three-gas incubator after the three-gas incubator, the original setting environment is restored, a large amount of air enters the incubator at the time of opening and closing the door, the rats re-inhale oxygen, the original anoxic state is interrupted, the modeling success rate of the cerebral palsy model is reduced, and the uniformity of experiments is also greatly reduced.
In this regard, the inventors have made a verification test, and the verification result is as follows: when the door is opened and closed for the first time, the anoxic environment in the three-gas incubator is restored to the original 8 percent O 2 And 92% N 2 The required time is 3-5 min; the second time of opening and closing the door is longer, 5-8 min is needed, the most suitable hypoxia time for cerebral palsy modeling is 1.2-1.5 h, and the best hypoxia environment is restored to occupy at least one tenth of the time. Therefore, frequent opening and closing of the door inevitably leads to inconsistent and failed experimental results, and has a great influence on the success rate of the experiment.
In addition to the oxygen concentration, the temperature effect is also critical, and the optimum cerebral palsy modeling temperature is 37 ℃, and when the temperature is 36 ℃, the result is different from the standard cerebral palsy modeling result. The inventors made a related study: 6 SD rats (Latin school name: sprague-Dawley) with age of 7 days were subjected to relevant pathological examination at 25 ℃ for 6 hours, and no essential ischemia symptoms of cerebral palsy animals were found, and the influence of temperature on the experiment was extremely large.
Therefore, the inventor realizes that the existing laboratory incubator is high in price and limited in number of installed models, and the problem that the culture condition of the model at the moment of opening the door is destroyed by frequently opening and closing the door also exists in the using process, so that animals are oxygenated in certain time periods with constant hypoxia, and the oxygen quantity of the frequently opened and closed door is not controlled sufficiently, so that the temperature loss is serious. Because of the defects, the consistency difference of the behaviours and pathological results of the animals after the animals are molded by using the existing incubator is large. Therefore, the existing laboratory incubator has a plurality of defects in use, and the success rate of cerebral palsy model modeling, the experimental uniformity and the experimental result are affected.
Therefore, a new solution is needed to solve the problems existing in the prior art.
Disclosure of Invention
The application provides a cerebral palsy model incubator for solve current cerebral palsy model modeling and cultivate the instrument and can be passive make the animal keep invariable oxygen deficiency's certain time quantum oxygenation in the in-service use, lead to current cerebral palsy model modeling low success rate's problem.
In order to achieve the above object, the present application provides the following technical solutions:
the application provides a cerebral palsy model incubator, which comprises a box body, wherein a culture cavity is formed in the box body, a ventilation port communicated with the culture cavity is arranged on the wall of the box body, and a sensor for detecting the concentration of a gas component is arranged in the culture cavity;
one or more push-pull channels are formed in the side wall of the culture chamber and used for accommodating the culture bin, the culture bin comprises a model accommodating groove, end covers are respectively arranged at two ends of the model accommodating groove, sealing elements are arranged on the end covers, one end of the culture bin extends into the culture chamber along the push-pull channels, and the other end of the culture bin is accommodated in the push-pull channels and seals the push-pull channels through the sealing elements;
a bracelet is arranged on an end cover accommodated in the push-pull channel; an end cover extending into the culture chamber is provided with an electric connection assembly, the bottom of the model accommodating groove is provided with a heating pad, an electric wire of the heating pad is connected with one end of the electric connection assembly, and the other end of the electric connection assembly is connected with a power wire;
the top of the model accommodating groove is detachably connected with a mesh cover, and the model accommodating groove is communicated with the culture chamber through meshes on the mesh cover.
In the above technical scheme, a controller is arranged on the box body, and the sensor is connected with the controller through signals.
Further, the sensor comprises an oxygen concentration detection sensing module and a carbon dioxide concentration detection sensing module.
Further, a switch door is arranged on the box body and is rotationally connected with the edge of the side wall provided with the push-pull channel; the switch door comprises an outer door leaf and an inner door leaf, the inner door leaf is a transparent door plate, a display screen is arranged on the outer wall of the outer door leaf, which is away from the inner door leaf, the display screen is connected with the controller, and the concentration value of the gas component detected by the sensor is displayed on the display screen.
Further, a strip-shaped groove cavity is formed in the model accommodating groove, a heating pad is arranged in the groove cavity, and a waterproof pad is arranged on the heating pad.
Further, the thickness range of the side wall provided with the push-pull channel is one third to one half of the axial length of the culture bin.
Further, the two end covers on the culture bin are the same in size and shape; the end cover is a circular plastic end cover, the end cover and the model accommodating groove are integrally formed, a rubber ring is sleeved on the end cover, and the end cover seals the push-pull channel through the rubber ring on the end cover; the shape and the size of the push-pull channel are matched with those of the end cover sleeved with the rubber ring.
Further, the two end covers on the culture bin are the same in size and shape; the two end covers are cylindrical rubber blocks, clamping grooves matched with the end parts of the model accommodating grooves are formed in the rubber blocks, and the end parts of the model accommodating grooves are embedded in the clamping grooves; a power-on assembly is embedded in the rubber block, plug wire holes are formed in two ends of the power-on assembly, and the electric wires of the heating pad and the power supply electric wires are correspondingly connected with one plug wire hole respectively.
Further, the size of the mesh hole cover is adaptively connected with the groove top of the model accommodating groove, one side of the mesh hole cover is rotationally connected with the groove wall of the model accommodating groove, a rotating connecting seat is arranged at the edge of the groove wall, which is close to the groove top, and a rotating shaft which is adaptively connected with the rotating connecting seat is arranged at one side of the mesh hole cover; the other side of the mesh hole cover is provided with a buckle plate, and the wall of the groove is provided with a clamping seat matched with the buckle plate.
Further, the cross-sectional area of the mold receiving groove is smaller than the cross-sectional area of the end cover.
Further, one end of the push-pull channel is provided with an extension channel, one end of the extension channel is connected to the inner wall of the culture chamber, and the extension channel is communicated with the push-pull channel and forms a moving track of the culture bin; the extension channel is a pipeline with a semicircular cross section, which is connected with the end part of the push-pull channel.
Further, a temperature sensor is arranged in the model accommodating groove and connected with a controller, and the controller is used for controlling the heating state of the heating pad according to the target set temperature and the actually measured temperature.
Further, a water bath tray is arranged in the culture chamber, and a temperature sensor for monitoring the real-time temperature of the culture chamber is arranged on the inner wall of the culture chamber.
Further, the model accommodating groove is of a cuboid thin-shell structure, and the length, the width and the height of the model accommodating groove are respectively 50cm, 25cm and 20cm.
Further, the end cover is cylindrical, the diameter of the end cover is 30cm, and the height of the end cover is 5cm.
The application provides a cerebral palsy model incubator, which comprises a case body, wherein a culture chamber is formed in the case body, and the culture chamber is filled with low-oxygen mixed gas; one or more push-pull channels are formed in the side wall of the culture chamber, a culture bin is arranged in each push-pull channel, each culture bin comprises a model accommodating groove, end covers are respectively arranged at two ends of each model accommodating groove, sealing elements are arranged on the end covers, one end of each culture bin extends into the culture chamber along the push-pull channel, and the other end of each culture bin is accommodated in the push-pull channel and seals the push-pull channel through the sealing element on the push-pull channel; each culture bin is an independent bin for culturing the cerebral palsy model, so that the frequent opening and closing of the door does not affect the gas environment in each culture bin, and the problem of low success rate of modeling of the traditional cerebral palsy model caused by oxygenation of animals in certain time periods of continuous constant hypoxia after the frequent opening and closing of the door of the traditional three-gas incubator is solved; in addition, an end cover extending into the culture chamber is provided with an electric connection assembly, the bottom of the model accommodating groove is provided with a heating pad, an electric wire of the heating pad is connected with one end of the electric connection assembly, and the other end of the electric connection assembly is connected with a power wire; the top of model accommodation groove can be dismantled and be connected with the mesh lid, and the model accommodation groove is through mesh and the cultivate cavity gas intercommunication on the mesh lid, and the mesh lid can prevent that animal model from running out. Therefore, the cerebral palsy model incubator provided by the application can keep the constant temperature of the animal model in the model accommodating groove, ensure the constant modeling temperature and improve the modeling success rate of the cerebral palsy model.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. It should be understood that the specific shape and configuration shown in the drawings should not be considered in general as limiting upon the practice of the present application; for example, based on the technical concepts and exemplary drawings disclosed herein, those skilled in the art have the ability to easily make conventional adjustments or further optimizations for the add/subtract/assign division, specific shapes, positional relationships, connection modes, dimensional scaling relationships, etc. of certain units (components).
FIG. 1 is a schematic structural view of a part of the structure of a cerebral palsy model incubator provided in the present application, showing 9 push-pull channels, in which only the two push-pull channels of the uppermost row are inserted with culture bins, and the culture bins are completely inserted into the push-pull channels;
FIG. 2 is a schematic structural view of a cerebral palsy model incubator according to an embodiment of the present disclosure, where the schematic structural view shows a state in which two culture chambers are completely pulled out of a push-pull channel, and is mainly used to show a mounting relationship between the culture chambers and the push-pull channel, and no mesh hole cover is shown on the culture chambers in the figure;
FIG. 3 is a schematic cross-sectional view of a cerebral palsy model incubator according to one embodiment of the present application, mainly illustrating the positional relationship between an extension channel and a push-pull channel;
FIG. 4 is a schematic view of the structure of a culture chamber of the cerebral palsy model incubator provided in the present application, in which the mesh cover is not shown, according to one embodiment.
Reference numerals illustrate:
1. a case; 2. a culture chamber; 3. a push-pull channel; 4. an extension channel; 5. a culture bin; 51. a model accommodating groove; 52. an end cap; 53. a mesh cover; 54. a bracelet; 6. an outer door leaf; 7. an inner door leaf; 8. and (5) a water bath tray.
Detailed Description
The present application is further described in detail below with reference to the attached drawings.
In the description of the present application: unless otherwise indicated, the meaning of "a plurality" is two or more. The terms "first," "second," "third," and the like in this application are intended to distinguish between the referenced objects without a special meaning in terms of technical connotation (e.g., should not be construed as emphasis on degree or order of importance, etc.). The expressions "comprising", "including", "having", etc. also mean "not limited to" (certain units, components, materials, steps, etc.).
The terms such as "upper", "lower", "left", "right", "middle", and the like, as referred to in this application, are generally used for convenience in visual understanding with reference to the drawings, and are not intended to be an absolute limitation of the positional relationship in actual products. Such changes in relative positional relationship are considered to be within the scope of the present description without departing from the technical concepts disclosed herein.
Example 1
In order to solve the problems in the prior art, the application provides a cerebral palsy model incubator, see fig. 1 and 3, which comprises a box body 1, wherein a culture chamber 2 for culturing an experimental animal cerebral palsy model is formed in the box body 1, and a hypoxia mixed gas is filled in the culture chamber 2 to manufacture an anoxic environment. One or more push-pull channels 3 are arranged on the side wall of the culture chamber 2, a culture bin 5 is accommodated in each push-pull channel 3, each culture bin 5 is a separate bin for culturing a cerebral palsy model, and a sealing piece for sealing the push-pull channel 3 is arranged on each culture bin 5. Therefore, whether the side wall provided with the push-pull channel 3 is provided with the opening and closing door of the box body 1 or not, the anoxic environment of the incubator is not affected. Therefore, the cerebral palsy model incubator solves the problem that in the prior art, the problem that the modeling result is affected by oxygenation of animals in certain time periods of continuous constant hypoxia caused by frequent door opening and closing is solved.
The cerebral palsy model incubator provided by the application can be provided with a plurality of culture bins 5, so that one or more experimental animal models in the same batch can be stored. In an embodiment, the number can be further carried out on each cultivating bin 5, corresponding indicator lamps are arranged on each cultivating bin 5, the indicator lamps of the cultivating bins 5 with the models are on, the indicator lamps of the empty cultivating bins 5 are not on, and the experimenters can know the service conditions of each cultivating bin 5 according to the indicator lamps.
The culture chamber 2 of the cerebral palsy model incubator provided by the application is tetragonal. In one embodiment, a plurality of push-pull channels 3 may be provided on each side wall of the culture chamber 2 to increase the rate of accommodation of the culture compartment 5 of the cerebral palsy model incubator. Of course, a plurality of push-pull channels 3 may be provided on a certain side wall of the culture chamber 2, and the culture chamber may be manufactured according to the user's needs during the manufacturing process.
The utility model provides a be provided with the port of ventilating that communicates with cultivateing cavity 2 on the box 1 case wall of cerebral palsy model incubator, be provided with sealed lid on the port of ventilating, seal the port of ventilating with sealed lid after the inflation. In this example, the mixed gas of low oxygen introduced into the culture chamber 2 through the introduction port was composed of 6% oxygen and 94% nitrogen.
The utility model provides a be provided with the sensor that is used for detecting gaseous component concentration in the culture chamber 2 of cerebral palsy model incubator, the sensor includes oxygen concentration detection sensing module and carbon dioxide concentration detection sensing module. A controller may be provided on the housing 1, with the sensor being in signal communication with the controller. The controller may convert the signal detected by the sensor into a corresponding concentration value.
In this embodiment, a door can be provided on the edge of the side wall provided with the push-pull channel 3, the door can be turned on and off relative to the side wall, a display screen can be provided on the door, the display screen is connected with the controller, the concentration value of the gas component detected by the sensor is displayed on the display screen, and alarm information can be displayed on the display screen, for example, when the concentration value of the gas is not in the range of the target concentration value, alarm prompt can be performed.
In one embodiment, see fig. 1, the door comprises an outer door leaf 6 and an inner door leaf 7, the inner door leaf 7 being a transparent door leaf, a display being provided on the outer wall of the outer door leaf 6 facing away from the inner door leaf 7. The transparent door plate has the functions of insulation and secondary sealing to a certain extent, and can be a glass door. The outer door leaf 6 not only plays a role in sealing but also plays a role in data display.
In this embodiment, referring to fig. 4, the culture chamber 5 accommodated in the push-pull channel 3 includes a model accommodating groove 51, two ends of the model accommodating groove 51 are respectively provided with an end cover 52, the end cover 52 is provided with a sealing member, one end of the culture chamber 5 extends into the culture chamber 2 along the push-pull channel 3, and the other end is accommodated in the push-pull channel 3 and seals the push-pull channel 3 by the sealing member thereon; a bracelet 54 is arranged on the end cover 52 accommodated in the push-pull channel 3; the end cover 52 extending into the culture chamber 2 is provided with an electric connection assembly, the bottom of the model accommodating groove 51 is provided with a heating pad, an electric wire of the heating pad is connected with one end of the electric connection assembly, and the other end of the electric connection assembly is connected with a power wire. The heating pad can keep the constant temperature of the culture bin 5 according to the set temperature. Preferably, a temperature sensor may be disposed in the mold accommodating groove 51, and the temperature sensor is connected to a controller, and the controller may control the heating state of the heating pad according to the target set temperature and the measured temperature, for example, control the heating pad to continue heating, or control the heating pad to stop heating. During the experiment, the heating pad can be preheated.
To prevent the experimental animal model from escaping, referring to fig. 3, a mesh cover 53 is detachably coupled to the top of the model receiving groove 51, and the model receiving groove 51 is in gas communication with the culture chamber 2 through the mesh of the mesh cover 53.
Therefore, the culture bin 5 provided by the application can be in gas communication with the culture chamber 2 through the mesh cover 53, and the push-pull channel 3 can be sealed through the sealing piece on the end cover 52, so that the gas in the culture chamber 2 can not leak when the culture bin 5 is pulled.
In this embodiment, a strip-shaped groove cavity is formed in the mold accommodating groove 51 formed in the culture bin 5, a heating pad is arranged in the groove cavity, and a waterproof pad is arranged on the heating pad. The thickness of the side wall provided with the push-pull channel 3 ranges from one third to one half of the axial length of the culture bin 5.
One end of the push-pull channel 3 is provided with an extension channel 4, one end of the extension channel 4 is connected to the inner wall of the culture chamber 2, and the extension channel 4 is communicated with the push-pull channel 3 and forms a moving track of the culture bin 5; the extension channel 4 is a pipe with a semicircular cross section connected to the end part of the push-pull channel 3.
The two end caps 52 on the culture bin 5 are the same in size and shape, so that the two end caps 52 are matched with the push-pull channel 3 in the push-pull process of the culture bin 5, and the push-pull channel 3 is sealed.
In one embodiment, the end cover 52 is a circular plastic end cover 52, the end cover 52 and the model accommodating groove 51 are integrally formed, at this time, the model accommodating groove 51 and the end cover 52 are both plastic pieces, a rubber ring is sleeved on the end cover 52, and the end cover 52 seals the push-pull channel 3 through the rubber ring thereon; the shape and size of the push-pull channel 3 are adapted to the shape and size of the end cap 52 provided with a rubber ring.
A plastic end cover 52 can be integrally provided with a hand ring 54, so that the cultivating cabin 5 is conveniently pushed and pulled; the other plastic end cap 52 may be provided with a mounting groove for mounting the electrical connection assembly, and after the electrical connection assembly is mounted, in order to realize sealing of the end cap 52, a sealing ring may be disposed at a place where the electrical connection assembly and the end cap 52 are mounted.
In another embodiment, both end caps 52 are cylindrical rubber blocks, and the rubber blocks are formed with clamping grooves adapted to the ends of the mold accommodating grooves 51, and the ends of the mold accommodating grooves 51 are embedded in the clamping grooves. A power-on assembly is embedded in the rubber block, plug wire holes are formed in two ends of the power-on assembly, and the electric wires of the heating pad and the power supply electric wires are correspondingly connected with one plug wire hole respectively. A hand ring 54 can be integrally arranged on the other rubber block, so that the push-pull culture bin 5 is convenient.
In this embodiment, the size of the mesh cover 53 is adaptively connected with the top of the mold accommodating groove 51, one side of the mesh cover 53 is rotationally connected with the groove wall of the mold accommodating groove 51, the edge of the groove wall, which is close to the top of the groove, is provided with a rotational connecting seat, and one side of the mesh cover 53 is provided with a rotating shaft adaptively connected with the rotational connecting seat; the other side of the mesh hole cover 53 is provided with a buckle plate, and the slot wall is provided with a clamping seat matched with the buckle plate. The connection structure is designed mainly to fix the mesh cover 53 and prevent animals from coming out of the model accommodating groove 51. Of course, the connection structure of the mesh cover 53 and the mold receiving groove 51 may be changed as long as the opening and locking are facilitated.
In order to achieve sealing of the end cap 52 and installation of the mold receiving groove 51, the cross-sectional area of the mold receiving groove 51 is smaller than that of the end cap 52.
In a preferred embodiment, the mold accommodating groove 51 has a rectangular thin shell structure, and the entire length, width and height of the mold accommodating groove 51 are 50cm, 25cm and 20cm, respectively, and a rectangular groove cavity is formed in the mold accommodating groove 51. The end cap 52 is cylindrical, the diameter of the end cap 52 is 30cm, and the height of the end cap 52 is 5cm. Of course, the dimensions of the model receiving groove 51 and the end cap 52 can be adjusted according to the size of the animal model.
In another embodiment, referring to fig. 4, the mold receiving groove 51 may be a semi-cylindrical shell structure with the same diameter as the end cap, and a rectangular parallelepiped groove cavity is formed in the semi-cylindrical mold receiving groove 51. The shape of the mold accommodating groove 51 in the culture chamber in the present application may be any shape as long as it is suitable for the purpose of culturing and molding.
The cerebral palsy model incubator that this application provided when using, preheat the heating pad earlier, then open the switch door of incubator, drag bracelet 54 on the end cover 52 and pull out culture bin 5, when culture bin 5 was pulled out to push-and-pull passageway 3 port position, sealing member on the end cover 52 can play sealed effect, prevent inside gas escape outside gas entering the inside, then put into model accommodation groove 51 with experimental animals after, cover the wire hole cover 53, inwards push in culture bin 5, push-and-pull passageway 3 can be plugged up to end cover 52, prevent gas escape or get into cultivate cavity 2, then take out experimental animals after waiting for the stipulation time.
The cerebral palsy model incubator provided by the application can be customized to a proper size according to requirements, such as a small incubator 1 capable of accommodating a plurality of experimental animal models, or a middle incubator 1 capable of accommodating tens or more experimental animal models, and the like. Compared with a three-gas incubator used in the laboratory at present, the incubator provided by the application can carry out sealing culture on one or more experimental animal models in the same batch, when a new experimental action model needs sealing culture, the incubator can be additionally arranged in other culture bins 5, so that the problem that the existing high-capacity three-gas incubator can oxygenate in a certain time period when an experimenter opens and closes a door to ensure that the animal is continuously and constantly anoxic after sealing culture, the anoxic state and the temperature are always kept constant, the molding success rate of a cerebral palsy model is improved, and the high consistency of experimental operation is improved.
Example two
Compared with the cerebral palsy model incubator provided in the first embodiment, the cerebral palsy model incubator provided in the first embodiment is filled with the hypoxia mixed gas consisting of 8% of oxygen and 92% of nitrogen.
Example III
Compared with the cerebral palsy model incubator provided in the first embodiment, the cerebral palsy model incubator provided in the first embodiment is filled with the hypoxia mixed gas consisting of 10% of oxygen and 90% of nitrogen.
Example IV
According to the embodiment, based on the structure of the cerebral palsy model incubator provided by the first embodiment, the water bath tray 8 is arranged in the culture chamber 2, the temperature sensor for monitoring the real-time temperature of the culture chamber 2 is arranged on the inner wall of the culture chamber 2, and the temperature sensor is connected with the controller.
In order to realize autonomous placement of the water bath tray 8, the side walls of the culture chamber may be configured to be of a door type structure capable of being opened or closed, for example, the tetragonal culture chamber 2 has four side walls, a top wall and a bottom wall, any one of the four side walls may be configured to be a door capable of being opened or closed, a rotary door may be configured to be a sliding door, and sealing structures such as sealing strips are required to be arranged at the peripheral edges of the door to ensure the air tightness of the culture chamber 2.
The water bath tray 8 provided in this embodiment may be placed in the culture chamber 2 at the time of cytological experiments.
In this embodiment, the bottom of the water bath tray 8 may be provided with a heating plate, the heating plate is connected to a controller, and the controller may control whether the heating plate heats water in the water bath tray 8 according to the temperature monitored by the temperature sensor.
In this embodiment, a water-adding pipe may be further disposed on the side wall of the culture chamber 2, a sealing ring is disposed between the water-adding pipe and the side wall, and a sealing cover is disposed at the end portion of the water-adding pipe exposed out of the culture chamber 2.
In this embodiment, the cerebral palsy model incubator with the water bath tray 8 can be used for culturing cerebral palsy models and performing cytology experiments.
In a preferred embodiment, the mold accommodating groove is of a cuboid thin-shell structure, and the overall length, width and height of the mold accommodating groove are respectively 50cm, 25cm and 20cm. The end cover is cylindrical, the diameter of the end cover is 30cm, and the height of the end cover is 5cm. When cytology experiments are needed, the design length and the height can be adapted to 150cm 2 Cell culture flasks.
Any combination of the technical features of the above embodiments may be performed (as long as there is no contradiction between the combination of the technical features), and for brevity of description, all of the possible combinations of the technical features of the above embodiments are not described; these examples, which are not explicitly written, should also be considered as being within the scope of the present description.
The foregoing has outlined and detailed description of the present application in terms of the general description and embodiments. It should be appreciated that numerous conventional modifications and further innovations may be made to these specific embodiments, based on the technical concepts of the present application; but such conventional modifications and further innovations may be made without departing from the technical spirit of the present application, and such conventional modifications and further innovations are also intended to fall within the scope of the claims of the present application.
Claims (10)
1. The cerebral palsy model incubator is characterized by comprising a box body, wherein a culture cavity is formed in the box body, a ventilation port communicated with the culture cavity is formed in the wall of the box body, and a sensor for detecting the concentration of a gas component is arranged in the culture cavity;
one or more push-pull channels are formed in the side wall of the culture chamber and used for accommodating the culture bin, the culture bin comprises a model accommodating groove, end covers are respectively arranged at two ends of the model accommodating groove, sealing elements are arranged on the end covers, one end of the culture bin extends into the culture chamber along the push-pull channels, and the other end of the culture bin is accommodated in the push-pull channels and seals the push-pull channels through the sealing elements;
a bracelet is arranged on an end cover accommodated in the push-pull channel; an end cover extending into the culture chamber is provided with an electric connection assembly, the bottom of the model accommodating groove is provided with a heating pad, an electric wire of the heating pad is connected with one end of the electric connection assembly, and the other end of the electric connection assembly is connected with a power wire;
the top of model accommodation groove can be dismantled and be connected with the mesh lid, the model accommodation groove is through mesh on the mesh lid and cultivate cavity gas intercommunication.
2. The cerebral palsy model incubator according to claim 1, wherein a controller is arranged on the incubator body, and the sensor is in signal connection with the controller;
the sensor comprises an oxygen concentration detection sensing module and a carbon dioxide concentration detection sensing module;
the box body is provided with a switch door which is rotationally connected with the edge of the side wall provided with the push-pull channel;
the switch door comprises an outer door leaf and an inner door leaf, the inner door leaf is a transparent door plate, a display screen is arranged on the outer wall of the outer door leaf, which is away from the inner door leaf, the display screen is connected with a controller, and the concentration value of the gas component detected by the sensor is displayed on the display screen.
3. The cerebral palsy model incubator according to claim 1, wherein a strip-shaped groove cavity is formed in the model accommodating groove, the heating pad is arranged in the groove cavity, and a waterproof pad is arranged on the heating pad;
the thickness range of the side wall provided with the push-pull channel is one third to one half of the axial length of the culture bin.
4. The cerebral palsy model incubator of claim 1, wherein both of the end caps on the incubator's bin are the same size and shape;
the end cover is a round plastic end cover, the end cover and the model accommodating groove are integrally formed, a rubber ring is sleeved on the end cover, and the end cover seals the push-pull channel through the rubber ring on the end cover;
the shape and the size of the push-pull channel are matched with those of the end cover sleeved with the rubber ring.
5. The cerebral palsy model incubator of claim 1, wherein both of the end caps on the incubator's bin are the same size and shape; the two end covers are cylindrical rubber blocks, clamping grooves matched with the end parts of the model accommodating grooves are formed in the rubber blocks, and the end parts of the model accommodating grooves are embedded in the clamping grooves;
and one of the rubber blocks is embedded with an electric connection assembly, two ends of the electric connection assembly are respectively provided with a plug wire hole, and the electric wires of the heating pad and the power supply electric wires are respectively and correspondingly connected with one plug wire hole.
6. The cerebral palsy model incubator according to claim 1, wherein the size of the mesh cover is adaptively connected with the groove top of the model accommodating groove, one side of the mesh cover is rotationally connected with the groove wall of the model accommodating groove, a rotating connecting seat is arranged at the edge of the groove wall, which is close to the groove top, and a rotating shaft which is adaptively connected with the rotating connecting seat is arranged at one side of the mesh cover; the other side of the wire hole cover is provided with a buckle plate, and the slot wall is provided with a clamping seat matched with the buckle plate.
7. The cerebral palsy model incubator of claim 1, wherein the cross-sectional area of the model receiving groove is smaller than the cross-sectional area of the end cap;
one end of the push-pull channel is provided with an extension channel, one end of the extension channel is connected to the inner wall of the culture chamber, and the extension channel is communicated with the push-pull channel and forms a moving track of the culture bin; the extension channel is a pipeline with a semicircular cross section, which is connected to the end part of the push-pull channel.
8. The cerebral palsy model incubator according to claim 2, wherein a temperature sensor is disposed in the model accommodating groove, the temperature sensor is connected to a controller, and the controller is configured to control a heating state of the heating pad according to a target set temperature and an actually measured temperature.
9. The cerebral palsy model incubator according to claim 1, wherein a water bath tray is arranged in the culture chamber, and a temperature sensor for monitoring the real-time temperature of the culture chamber is arranged on the inner wall of the culture chamber.
10. The cerebral palsy model incubator according to claim 1, wherein the model accommodating groove is of a cuboid thin-shell structure, and the length, width and height of the model accommodating groove are respectively 50cm, 25cm and 20cm;
the end cover is cylindrical, the diameter of the end cover is 30cm, and the height of the end cover is 5cm.
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| CN113088451A (en) * | 2021-04-21 | 2021-07-09 | 云南中医药大学 | Biological incubator for experiments |
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| CN209024556U (en) * | 2018-10-29 | 2019-06-25 | 代玉红 | Test-tube baby's Embryo Culture case |
| CN210127244U (en) * | 2019-04-19 | 2020-03-06 | 广州医科大学附属第一医院 | Cell oxygen deficiency incubator |
| CN110628707A (en) * | 2019-11-01 | 2019-12-31 | 浙江大学 | Culture method for improving in vitro survival rate of mammalian embryo |
| CN112852609A (en) * | 2021-02-03 | 2021-05-28 | 周桂珍 | Lactic acid bacteria separation screening culture system |
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