CN116391810A - Fermented ration for reducing weight of cats and preparation method thereof - Google Patents
Fermented ration for reducing weight of cats and preparation method thereof Download PDFInfo
- Publication number
- CN116391810A CN116391810A CN202310492558.5A CN202310492558A CN116391810A CN 116391810 A CN116391810 A CN 116391810A CN 202310492558 A CN202310492558 A CN 202310492558A CN 116391810 A CN116391810 A CN 116391810A
- Authority
- CN
- China
- Prior art keywords
- parts
- fermented
- pumpkin
- eurotium cristatum
- food
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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Classifications
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
- A23K50/42—Dry feed
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
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- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
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- A—HUMAN NECESSITIES
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C—CHEMISTRY; METALLURGY
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention provides a fermented daily ration for cat weight loss and a preparation method thereof, wherein pumpkin is fermented by using Eurotium cristatum, and other raw materials and additive components are mixed, so that the physique of a pet can be improved, the occurrence of obesity is reduced, and the physical health of the pet is ensured. The pumpkin fermented by the Eurotium cristatum is added, so that the cat food has a certain weight-losing effect, and the pumpkin contains more dietary fibers, can soften feces to a certain extent, prevent constipation, promote intestinal absorption and the like. The animal protein content is high, the nutrition is rich, no grain is added, and the intestinal absorption of the pet is facilitated. The low-temperature baking requires that the product is kept at the core temperature of 90 ℃ and is baked slowly in an oven, so that the process can avoid a great loss of nutrition of fresh food materials, retain the original taste and flavor of the food materials, and does not need to additionally spray nutritional supplements and food inducers.
Description
Technical Field
The invention provides fermented ration for losing weight of cats and a preparation method thereof, relates to cat food and a preparation method thereof, and belongs to the technical field of animal feed production.
Background
With the development of economy in China, GDP (gas dynamic pressure) is improved year by year, pet economy is vigorously developed, and a large number of researches show that obesity of pet cats can seriously affect the appearance and animal welfare of the pet cats, reduce the quality of life, and have a plurality of actual and serious health risks, and can have a plurality of adverse effects on the health and the service life of the pet cats. Obesity itself has a close relationship with the occurrence of many diseases including osteoarticular diseases, diabetes, cardiopulmonary diseases, urinary system diseases, reproductive system diseases, metabolic diseases, tumors (breast tumors, transitional cell carcinoma), dermatological diseases, and anesthetic complications. In developed countries, veterinary communities have formed consensus on being a significant health problem for obesity in pets. With the intensive research of obesity problems, more and more people consider that obesity problems have become the first killer threatening the health of pets. The U.S. national chief researcher Ernie Ward doctor explicitly states that: "currently, the pet obesity problem is the greatest threat to the health of us pets.
Eurotium cristatum (Eurotium cristatum, E.cristatum) is also known as golden flower fungus, belongs to the phylum ascomycota, the order Ascomycetes, the order Aspergillus, the family Aspergillus, the genus Eurotium, is a dominant strain obtained by the process of "growing flowers" of Fuzhuan tea under certain temperature and humidity conditions. In the fermentation process of Fuzhuan tea, eurotium cristatum can secrete a plurality of extracellular enzymes, so that the fermented product has special flavor and color. The Eurotium cristatum not only can improve the color, fragrance and taste of Fuzhuan tea, but also can reduce cholesterol, resist oxidation and reduce blood sugar. Researches show that the Eurotium cristatum has the activities of resisting oxidation, inhibiting bacteria, reducing blood fat, producing enzymes and the like, and the fermentation of the Eurotium cristatum can improve the biological activity of a fermented product. Studies show that the fermentation product of the Eurotium cristatum contains lipid-lowering substances and can relieve the chronic kidney disease of high-fat rats by relieving skeletal muscle insulin resistance; enzymes in the fermentation liquor have an inhibiting effect on the activity of lipase, so that the digestion and metabolism of fat are prevented, and the effect of losing weight is achieved; in addition, the fermentation product obtained by the Eurotium cristatum has certain anti-inflammatory potential and anti-tumor activity.
At present, the technical development of China on the pet staple food is very different, but the development of functional cat food, especially cat food with the weight-losing effect is less.
Disclosure of Invention
The invention aims to provide a fermented ration for reducing weight of cats and a preparation method thereof, wherein pumpkin is fermented by using Eurotium cristatum, and other raw materials and additive components are mixed, so that the physique of the pets can be improved, the occurrence of obesity is reduced, and the physical health of the pets is ensured.
In order to achieve the above object, the present invention provides the following solutions:
the invention provides a fermented ration for cat weight loss and a preparation method thereof, wherein the fermented ration comprises the following raw material components in parts by weight:
comprises 20-25 parts of frozen chicken breast, 15-20 parts of frozen chicken liver, 10-15 parts of frozen chicken heart, 3-5 parts of beef, 10-20 parts of salmon, 3-5 parts of black carp, 1-3 parts of green-mouth shellfish, 10-20 parts of pumpkin fermented by Eurotium cristatum, 8-10 parts of eggs, 0.1-0.3 part of cranberry powder, 0.1-0.5 part of vitamins, 0.05-0.1 part of food-grade preservative, 1-5 parts of cod-liver oil and 0.1-0.3 part of food additive.
The preparation method of the eurotium cristatum fermented pumpkin comprises the following steps:
sequentially mixing maltose culture medium and agar powder, adding into distilled water, mixing and sterilizing to obtain maltose agar culture medium, inoculating Eurotium cristatum into the maltose agar culture medium, culturing at 25-30deg.C for 3-7 days, scraping Aspergillus cristatus spores from flat plate with sterilized cell scraper after Eurotium cristatum forms ascospores, counting with blood cell counting method to obtain 10 7 -10 9 Peronosporangium coronatine spore suspension of/mL.
Peeling fructus Cucurbitae Moschatae, removing seeds, pulverizing, sealing, sterilizing with 121deg.C high pressure steam for 20min, and making into fructus Cucurbitae Moschatae culture medium. Inoculating the Eurotium cristatum suspension into a pumpkin culture medium according to the inoculation amount of 1-2% by weight, fermenting at the constant temperature of 30 ℃ for 15 days, and finishing the fermentation.
According to the physiological structure and eating habit of the pet cat, the animal source raw material accounts for more than 70% in the daily ration formula provided by the application, so that the physiological requirement of the pet cat can be well met, and furthermore, the pumpkin fermented by the Eurotium cristatum can play the roles of resisting oxidation, inhibiting bacteria, reducing blood fat and the like, and the dietary fiber in the pumpkin can promote intestinal absorption, soften excrement and improve the excrement quantity.
The invention also discloses a fermented ration for reducing weight of cats and a preparation method thereof, and the specific steps are as follows:
s1, pretreatment of raw materials: firstly, preparing ingredients according to the proportion of raw materials, thawing the frozen chicken breast, the frozen chicken liver and the frozen chicken heart at the temperature of-4-4 ℃ for 8-10 hours; chopping chicken breast, chicken heart, chicken liver, beef, salmon, black carp and the like into paste after thawing, adding eggs into the paste, and obtaining a raw material mixture by pumpkin, cranberry powder and cod liver oil fermented by Eurotium cristatum;
s2, forming: mixing and stirring the raw material mixture, the food-grade preservative and the food additive uniformly to obtain a final mixture, injecting the final mixture into a mold for molding, and placing the final mixture into an oven for preliminary dehydration at the temperature of 85-90 ℃ for 1-2h to obtain granular semi-finished cat food;
s3, shaping: and (3) carrying out secondary dehydration on the semi-finished feed, wherein the temperature is 75-80 ℃, the time period is 6-7 hours, and the moisture content of the semi-finished feed is monitored in real time, and the drying is finished when the moisture content is lower than 10%.
Furthermore, the drying oven adopts a fan circulation air supply mode, the air circulation is uniform and efficient, the air source drives the wind wheel to pass through the heater by the circulation air supply motor, hot air is sent out, then passes through the air channel to the inner chamber of the drying oven, and the used air is sucked into the air channel to become the air source for recirculation, heating and use, so that the indoor temperature uniformity is ensured; when the temperature value swings due to door opening and closing actions, the air supply circulation system quickly restores the operation state until the set temperature value is reached.
Compared with the prior art, the invention can obtain the following technical effects: the pumpkin fermented by the Eurotium cristatum is added, so that the cat food has a certain weight-losing effect, and the pumpkin contains more dietary fibers, can soften feces to a certain extent, prevent constipation, promote intestinal absorption and the like. The animal protein content is high, the nutrition is rich, no grain is added, and the intestinal absorption of the pet is facilitated. The low-temperature baking requires that the product is kept at the core temperature of 90 ℃ and is baked slowly in an oven, so that the process can avoid a great loss of nutrition of fresh food materials, retain the original taste and flavor of the food materials, and does not need to additionally spray nutritional supplements and food inducers.
Description of the embodiments
In order to make the objects, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1
The invention also provides a preparation method of the eurotium cristatum fermented pumpkin, which comprises the following steps:
(1) Activation of Eurotium cristatum: sequentially mixing maltose culture medium and agar powderProportion 13: 5) Adding into distilled water, mixing, sterilizing, making into maltose agar medium, and mixing Eurotium cristatum according to 1×10 6 CFU is inoculated into the maltose agar culture medium, and the culture is inverted and carried out for 3 to 7 days at the constant temperature of 25 to 30 ℃.
(2) Preparation of a eurotium cristatum spore suspension: after the ascospores are formed by the ascomycetes coronarissis, the ascomycetes coronarissis spores are scraped from the flat plate by using a sterilized cell scraper, and counted by a blood cell counting method to prepare 107-109/mL of ascomycetes coronarissis spore suspension.
(3) Preparation of pumpkin culture medium: peeling fructus Cucurbitae Moschatae, removing seeds, pulverizing, sealing, sterilizing with 121deg.C high pressure steam for 20min, and making into fructus Cucurbitae Moschatae culture medium.
(4) Inoculating and fermenting: inoculating the Eurotium cristatum suspension into a pumpkin culture medium according to the inoculation amount of 1-2% by weight, fermenting at the constant temperature of 25-30 ℃ for 15 days, and finishing the fermentation.
Example 2
The invention provides a fermented ration for reducing weight of cats, which comprises the following raw materials in parts by weight:
20 parts of frozen chicken breast, 17 parts of frozen chicken liver, 13 parts of frozen chicken heart, 5 parts of beef, 15 parts of salmon, 4 parts of black carp, 2 parts of green-mouth shellfish, 15 parts of pumpkin fermented by Eurotium cristatum, 8 parts of eggs, 0.2 part of cranberry powder, 0.5 part of vitamins, 0.1 part of food-grade preservative, 1 part of cod-liver oil and 0.3 part of food additive.
The additive component is formed by mixing potassium iodide, taurine, sodium chloride and calcium carbonate in equal proportion; the vitamin is prepared by mixing vitamin A acetate, vitamin D3, DL-alpha-tocopherol acetate, vitamin K1, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, nicotinic acid, D-calcium pantothenate, cyanocobalamine, folic acid and D-biotin in equal proportion; the food-grade preservative comprises tea polyphenol, sodium citrate and potassium sorbate in a proportion of 1:1:1, and mixing the materials in proportion.
A preparation method of fermented ration for reducing weight of cats comprises the following steps:
s1, pretreatment of raw materials: firstly, preparing ingredients according to the proportion of raw materials, thawing the frozen chicken breast, the frozen chicken liver and the frozen chicken heart at the temperature of-4-4 ℃ for 8-10 hours; after thawing, chicken breast, chicken heart, chicken liver, beef, salmon, black carp and the like are chopped into meat paste, and eggs are added into the meat paste, so that pumpkin, cranberry powder and cod liver oil fermented by Eurotium cristatum prepared in example 1 are obtained to obtain a raw material mixture.
S2, forming: mixing and stirring the raw material mixture, the food-grade preservative and the food additive uniformly to obtain a final mixture, injecting the final mixture into a mold for shaping, putting the final mixture into an oven for preliminary dehydration at the temperature of 85-90 ℃ for 1-2 hours, so that the final mixture has certain hardness, and obtaining granular semi-finished daily ration for facilitating subsequent processing.
S3, shaping: demolding the semi-finished feed, performing secondary dehydration at 75-80 ℃ for 6-7h, monitoring the moisture content in real time, taking out and cooling to room temperature after the drying is finished, and packaging to obtain the daily ration.
Example 3
The invention provides a fermented ration for reducing weight of cats, which comprises the following raw materials in parts by weight:
25 parts of frozen chicken breast, 15 parts of frozen chicken liver, 10 parts of frozen chicken heart, 3 parts of beef, 17 parts of salmon, 5 parts of black carp, 3 parts of green-mouth shellfish, 10 parts of pumpkin fermented by Eurotium cristatum, 9 parts of eggs, 0.2 part of cranberry powder, 0.5 part of vitamins, 0.1 part of food-grade preservative, 2 parts of cod-liver oil and 0.3 part of food additive.
The additive component is formed by mixing potassium iodide, taurine, sodium chloride and calcium carbonate in equal proportion; the vitamin is prepared by mixing vitamin A acetate, vitamin D3, DL-alpha-tocopherol acetate, vitamin K1, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, nicotinic acid, D-calcium pantothenate, cyanocobalamine, folic acid and D-biotin in equal proportion; the food-grade preservative comprises tea polyphenol, sodium citrate and potassium sorbate in a proportion of 1:1:1, and mixing the materials in proportion.
A preparation method of fermented ration for reducing weight of cats comprises the following steps:
s1, pretreatment of raw materials: firstly, preparing ingredients according to the proportion of raw materials, thawing the frozen chicken breast, the frozen chicken liver and the frozen chicken heart at the temperature of-4-4 ℃ for 8-10 hours; after thawing, chicken breast, chicken heart, chicken liver, beef, salmon, black carp and the like are chopped into meat paste, and eggs are added into the meat paste, so that pumpkin, cranberry powder and cod liver oil fermented by Eurotium cristatum prepared in example 1 are obtained to obtain a raw material mixture.
S2, forming: mixing and stirring the raw material mixture, the food-grade preservative and the food additive uniformly to obtain a final mixture, injecting the final mixture into a mold for shaping, putting the final mixture into an oven for preliminary dehydration at the temperature of 85-90 ℃ for 1-2 hours, so that the final mixture has certain hardness, and obtaining granular semi-finished daily ration for facilitating subsequent processing.
S3, shaping: demolding the semi-finished feed, performing secondary dehydration at 75-80 ℃ for 6-7h, monitoring the moisture content in real time, taking out and cooling to room temperature after the drying is finished, and packaging to obtain the daily ration.
Example 4
The invention provides a fermented ration for reducing weight of cats, which comprises the following raw materials in parts by weight:
23 parts of frozen chicken breast, 13 parts of frozen chicken liver, 13 parts of frozen chicken heart, 4 parts of beef, 10 parts of salmon, 3 parts of black carp, 5 parts of green-mouth shellfish, 20 parts of pumpkin fermented by Eurotium cristatum, 8 parts of eggs, 0.2 part of cranberry powder, 0.5 part of vitamins, 0.1 part of food-grade preservative, 1 part of cod-liver oil and 0.3 part of food additive.
The additive component is formed by mixing potassium iodide, taurine, sodium chloride and calcium carbonate in equal proportion; the vitamin is prepared by mixing vitamin A acetate, vitamin D3, DL-alpha-tocopherol acetate, vitamin K1, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, nicotinic acid, D-calcium pantothenate, cyanocobalamine, folic acid and D-biotin in equal proportion; the food-grade preservative comprises tea polyphenol, sodium citrate and potassium sorbate in a proportion of 1:1:1, and mixing the materials in proportion.
A preparation method of fermented ration for reducing weight of cats comprises the following steps:
s1, pretreatment of raw materials: firstly, preparing ingredients according to the proportion of raw materials, thawing the frozen chicken breast, the frozen chicken liver and the frozen chicken heart at the temperature of-4-4 ℃ for 8-10 hours; after thawing, chicken breast, chicken heart, chicken liver, beef, salmon, black carp and the like are chopped into meat paste, and eggs are added into the meat paste, so that pumpkin, cranberry powder and cod liver oil fermented by Eurotium cristatum prepared in example 1 are obtained to obtain a raw material mixture.
S2, forming: mixing and stirring the raw material mixture, the food-grade preservative and the food additive uniformly to obtain a final mixture, injecting the final mixture into a mold for shaping, putting the final mixture into an oven for preliminary dehydration at the temperature of 85-90 ℃ for 1-2 hours, so that the final mixture has certain hardness, and obtaining granular semi-finished daily ration for facilitating subsequent processing.
S3, shaping: demolding the semi-finished feed, performing secondary dehydration at 75-80 ℃ for 6-7h, monitoring the moisture content in real time, taking out and cooling to room temperature after the drying is finished, and packaging to obtain the daily ration.
Experimental example 1
10 cats with the weight of 3-4kg and the age of one year are selected, and the cats are equally divided into two groups, namely a control group and a test group, wherein the control group is fed with common full-price daily ration sold in the market, and the test group is fed with the fermented daily ration.
The initial weight of each group of cats was counted before the start of the test, the cats were fed free water during the test, the weight change of each group of cats was counted after one month, and the average weight was calculated. The results are shown in Table 1.
Table 1 average initial body weight, end body weight for each group.
The experimental result shows that the daily ration prepared by the invention has a certain weight-losing effect and can be suitable for the weight losing of cats. Cats fed the control diet all had a certain amount of weight gain. And through blood index detection, the working cats are in a healthy state.
Claims (3)
1. A fermented ration for reducing weight of cats is characterized by being mainly prepared from the following substances in parts by weight:
20-25 parts of frozen chicken breast, 15-20 parts of frozen chicken liver, 10-15 parts of frozen chicken heart, 3-5 parts of beef, 10-20 parts of salmon, 3-5 parts of black carp, 1-3 parts of green-mouth shellfish, 10-20 parts of pumpkin fermented by Eurotium cristatum, 8-10 parts of eggs, 0.1-0.3 part of cranberry powder, 0.1-0.5 part of vitamins, 0.05-0.1 part of food-grade preservative, 1-5 parts of cod-liver oil and 0.1-0.3 part of food additive.
2. The method for preparing fermented ration for weight loss in cats according to claim 1, comprising the following steps:
s1, pretreatment of raw materials: firstly, preparing ingredients according to the proportion of raw materials, thawing the frozen chicken breast, the frozen chicken liver and the frozen chicken heart at the temperature of-4-4 ℃ for 8-10 hours; chopping chicken breast, chicken heart, chicken liver, beef, salmon, black carp and the like into paste after thawing, adding eggs into the paste, and obtaining a raw material mixture by pumpkin, cranberry powder and cod liver oil fermented by Eurotium cristatum;
s2, forming: mixing and stirring the raw material mixture, the food-grade preservative and the food additive uniformly to obtain a final mixture, injecting the final mixture into a mold for molding, and placing the final mixture into an oven for preliminary dehydration at the temperature of 85-90 ℃ for 1-2h to obtain granular semi-finished cat food;
s3, shaping: and (3) carrying out secondary dehydration on the semi-finished feed, wherein the temperature is 75-80 ℃, the time period is 6-7 hours, and the moisture content of the semi-finished feed is monitored in real time, and the drying is finished when the moisture content is lower than 10%.
3. The method for preparing fermented daily ration for reducing weight of cats according to claim 1, wherein the method for preparing the pumpkin fermented by Eurotium cristatum is as follows:
(1) Activation of Eurotium cristatum: sequentially mixing a maltose culture medium and agar powder according to a proportion of 13: mixing at a ratio of 5, adding into distilled water, mixing, sterilizing, and making into maltose agar medium, mixing Eurotium cristatum at a ratio of 1×10 6 Inoculating CFU into the maltose agar culture medium, and culturing at 25-30deg.C for 3-7 days;
(2) Preparation of a eurotium cristatum spore suspension: after the ascospores are formed by the ascomycetes coronarissis, scraping the ascomycetes coronarissis spores from the flat plate by using sterilized cell scraping, and counting by using a blood cell counting method to prepare 107-109/mL ascomycetes coronarissis spore suspension;
(3) Preparation of pumpkin culture medium: peeling fructus Cucurbitae Moschatae, removing seeds, pulverizing, sealing, sterilizing with 121deg.C high pressure steam for 20min, and making into fructus Cucurbitae Moschatae culture medium;
(4) Inoculating and fermenting: inoculating the Eurotium cristatum suspension into a pumpkin culture medium according to the inoculation amount of 1-2% by weight, fermenting at the constant temperature of 25-30 ℃ for 15 days, and finishing the fermentation.
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CN104886303A (en) * | 2015-04-07 | 2015-09-09 | 安徽农业大学 | Preparation method of solid black tea beverage |
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CN112544810A (en) * | 2020-12-11 | 2021-03-26 | 杭州电子科技大学 | Cat food meeting nutritional requirements for growth of kittens and preparation method thereof |
CN115024421A (en) * | 2022-05-31 | 2022-09-09 | 上海奇偌宠物用品有限公司 | High-protein low-temperature baked cat food and preparation method thereof |
CN115968977A (en) * | 2022-12-26 | 2023-04-18 | 河南牧业经济学院 | Pet food for losing weight and preparation method thereof |
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CN104886303A (en) * | 2015-04-07 | 2015-09-09 | 安徽农业大学 | Preparation method of solid black tea beverage |
CN112205534A (en) * | 2020-10-12 | 2021-01-12 | 麦仕宠物食品(上海)有限公司 | Special nutritional food for improving obesity of sterilized cats and preparation method thereof |
CN112544810A (en) * | 2020-12-11 | 2021-03-26 | 杭州电子科技大学 | Cat food meeting nutritional requirements for growth of kittens and preparation method thereof |
CN115024421A (en) * | 2022-05-31 | 2022-09-09 | 上海奇偌宠物用品有限公司 | High-protein low-temperature baked cat food and preparation method thereof |
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