CN116348770A - 异天冬氨酸的检测方法 - Google Patents

异天冬氨酸的检测方法 Download PDF

Info

Publication number
CN116348770A
CN116348770A CN202180067521.7A CN202180067521A CN116348770A CN 116348770 A CN116348770 A CN 116348770A CN 202180067521 A CN202180067521 A CN 202180067521A CN 116348770 A CN116348770 A CN 116348770A
Authority
CN
China
Prior art keywords
protein
isoasp
peptide
fragmentation
mass spectrometry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180067521.7A
Other languages
English (en)
Chinese (zh)
Inventor
J·O-T·许
I·D·G·坎普萨诺
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of CN116348770A publication Critical patent/CN116348770A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
CN202180067521.7A 2020-10-01 2021-09-30 异天冬氨酸的检测方法 Pending CN116348770A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063086557P 2020-10-01 2020-10-01
US63/086,557 2020-10-01
US202163218186P 2021-07-02 2021-07-02
US63/218,186 2021-07-02
PCT/US2021/052783 WO2022072583A1 (en) 2020-10-01 2021-09-30 Methods of detecting isoaspartic acid

Publications (1)

Publication Number Publication Date
CN116348770A true CN116348770A (zh) 2023-06-27

Family

ID=78621972

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180067521.7A Pending CN116348770A (zh) 2020-10-01 2021-09-30 异天冬氨酸的检测方法

Country Status (10)

Country Link
US (1) US20230366889A1 (https=)
EP (1) EP4222500B1 (https=)
JP (1) JP2023546796A (https=)
CN (1) CN116348770A (https=)
AU (1) AU2021351498A1 (https=)
CA (1) CA3196920A1 (https=)
ES (1) ES3059573T3 (https=)
MX (1) MX2023003862A (https=)
TW (1) TWI907533B (https=)
WO (1) WO2022072583A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114144668B (zh) * 2019-07-17 2025-02-11 株式会社岛津制作所 异天冬氨酸的分析方法和质谱分析装置

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190073452A1 (en) * 2015-09-25 2019-03-07 Bioanalytix, Inc. Method for determining the in vivo comparability of a biologic drug and a reference drug
JP6665740B2 (ja) * 2016-09-13 2020-03-13 株式会社島津製作所 ペプチド解析方法、ペプチド解析装置、及びペプチド解析用プログラム
CN110779979A (zh) * 2019-10-09 2020-02-11 南昌大学 一种基于寡糖异构体组成差异鉴别蜂蜜糖浆掺假的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DEGRAAN-WEBER NICK等: "Distinguishing Aspartic and lsoaspartic Acids in Peptides by Several Mass Spectrometric Fragmentation Methods", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 9 September 2016 (2016-09-09), pages 2041 - 2053 *
LI YI等: "Isomerization of Asp–Asp Motif in Model Peptides and a Monoclonal Antibody Fab Fragment", JOURNAL OF PHARMACEUTICAL SCIENCES, 31 March 2013 (2013-03-31), pages 947 - 959 *

Also Published As

Publication number Publication date
EP4222500B1 (en) 2025-11-12
WO2022072583A1 (en) 2022-04-07
TWI907533B (zh) 2025-12-11
MX2023003862A (es) 2023-04-14
AU2021351498A1 (en) 2023-05-04
EP4222500C0 (en) 2025-11-12
CA3196920A1 (en) 2022-04-07
EP4222500A1 (en) 2023-08-09
TW202223377A (zh) 2022-06-16
ES3059573T3 (en) 2026-03-23
JP2023546796A (ja) 2023-11-08
AU2021351498A9 (en) 2025-03-27
US20230366889A1 (en) 2023-11-16

Similar Documents

Publication Publication Date Title
Jansson Strategies for analysis of isomeric peptides
JP5903051B2 (ja) ポリペプチドの配列バリアントの決定方法
US9181326B2 (en) Analysis of ubiquitinated polypeptides
Pham et al. De novo proteomic sequencing of a monoclonal antibody raised against OX40 ligand
Liu et al. Quantitation of asparagine deamidation by isotope labeling and liquid chromatography coupled with mass spectrometry analysis
WO2021180063A1 (en) Methods for detecting and quantifying biological modifications in antibodies
US11352418B2 (en) Analysis of ubiquitinated polypeptides
TWI907533B (zh) 異天冬胺酸之檢測方法
Wu et al. Localizing Isomerized Residue Sites in Peptides with Tandem Mass Spectrometry
US20240053359A1 (en) Native microfluidic ce-ms analysis of antibody charge heterogeneity
US20220326252A1 (en) Electron transfer dissociation and mass spectrometry for improved protein sequencing of monoclonal antibodies
JP2023530712A (ja) 未処理ｃ末端リジンを正確に測定するための重ペプチドアプローチ
KR20170071849A (ko) Lc-ms를 이용하는 항체 약물의 혈액 내 정량방법
JP2024540180A (ja) Ｍｓベースのプロテオミクスのためのジスルフィドスクランブルを防止するための方法
Gan Salt Free HILIC-MS/MS as a Comprehensive and Complementary Site-Specific Glycosylation Tool in Therapeutic Proteins, Expanding Structural Resolution of Glycan Isomers
EP4355771A1 (en) Anti-ubiquitination antibodies and methods of use
EP4402476A1 (en) Method to prevent sample preparation-induced disulfide scrambling in non-reduced peptide mapping
Du et al. MS Analysis of Biological Drugs, Proteins, and Peptides

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination