CN116334013A - 具有改进的特性的ω-羟化酶相关的融合多肽变体 - Google Patents

具有改进的特性的ω-羟化酶相关的融合多肽变体 Download PDF

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Publication number
CN116334013A
CN116334013A CN202210871940.2A CN202210871940A CN116334013A CN 116334013 A CN116334013 A CN 116334013A CN 202210871940 A CN202210871940 A CN 202210871940A CN 116334013 A CN116334013 A CN 116334013A
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seq
amino acid
cyp153a
fusion polypeptide
hybrid fusion
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CN202210871940.2A
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Chinese (zh)
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A·席尔默
B·朱
E·波波瓦
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Genomatica Inc
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Genomatica Inc
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
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    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
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    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
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    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01005—Lysophospholipase (3.1.1.5)
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    • C12Y301/02—Thioester hydrolases (3.1.2)
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)

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  • Oil, Petroleum & Natural Gas (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202210871940.2A 2015-12-15 2016-12-13 具有改进的特性的ω-羟化酶相关的融合多肽变体 Pending CN116334013A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
PCT/EP2015/079832 WO2017101987A1 (en) 2015-12-15 2015-12-15 Omega-hydroxylase-related fusion polypeptide variants with improved properties
EPPCT/EP2015/079832 2015-12-15
CN201680081302.3A CN108699536B (zh) 2015-12-15 2016-12-13 具有改进的特性的ω-羟化酶相关的融合多肽变体
PCT/US2016/066405 WO2017106205A1 (en) 2015-12-15 2016-12-13 Omega-hydroxylase-related fusion polypeptide variants with improved properties

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CN202210871940.2A Pending CN116334013A (zh) 2015-12-15 2016-12-13 具有改进的特性的ω-羟化酶相关的融合多肽变体
CN201680081302.3A Active CN108699536B (zh) 2015-12-15 2016-12-13 具有改进的特性的ω-羟化酶相关的融合多肽变体
CN202210859052.9A Pending CN116334012A (zh) 2015-12-15 2016-12-13 具有改进的特性的ω-羟化酶相关的融合多肽变体

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CN202210859052.9A Pending CN116334012A (zh) 2015-12-15 2016-12-13 具有改进的特性的ω-羟化酶相关的融合多肽变体

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EP (2) EP3390620B1 (enExample)
JP (2) JP6957469B2 (enExample)
KR (2) KR102840377B1 (enExample)
CN (3) CN116334013A (enExample)
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CA (1) CA3008836A1 (enExample)
ES (1) ES2908400T3 (enExample)
MX (1) MX2018007298A (enExample)
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MY203897A (en) 2013-06-14 2024-07-23 Genomatica Inc Methods of producing omega-hydroxylated fatty acid derivatives
US11441130B2 (en) 2014-06-16 2022-09-13 Genomatica, Inc. Omega-hydroxylase-related fusion polypeptides with improved properties
WO2017101987A1 (en) * 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
WO2019217226A1 (en) * 2018-05-10 2019-11-14 REG Life Sciences, LLC Multifunctional fatty acid derivatives and biosynthesis thereof
KR102516485B1 (ko) 2018-10-31 2023-04-03 삼성전자주식회사 폴더블 하우징을 포함하는 전자 장치

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SI3390620T1 (sl) 2022-05-31
KR20250117480A (ko) 2025-08-04
CA3008836A1 (en) 2017-06-22
US20190002845A1 (en) 2019-01-03
ES2908400T3 (es) 2022-04-29
JP2022002535A (ja) 2022-01-11
JP6957469B2 (ja) 2021-11-02
AU2016370490A1 (en) 2018-06-21
WO2017101987A1 (en) 2017-06-22
EP4026899A1 (en) 2022-07-13
US11384341B2 (en) 2022-07-12
US10787648B2 (en) 2020-09-29
MX2018007298A (es) 2019-01-10
BR112018012193A2 (pt) 2018-12-04
EP3390620B1 (en) 2022-01-05
US20210230562A1 (en) 2021-07-29
MY196584A (en) 2023-04-19
US12180515B2 (en) 2024-12-31
KR20180132604A (ko) 2018-12-12
KR102840377B1 (ko) 2025-08-01
EP3390620A1 (en) 2018-10-24
AU2016370490B2 (en) 2023-01-05
CN116334012A (zh) 2023-06-27
CN108699536B (zh) 2022-08-02
MY194277A (en) 2022-11-25
US20230159901A1 (en) 2023-05-25
JP7458358B2 (ja) 2024-03-29
WO2017106205A1 (en) 2017-06-22
CN108699536A (zh) 2018-10-23
JP2018537111A (ja) 2018-12-20

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