CN116333750A - Nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil and preparation method thereof - Google Patents

Nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil and preparation method thereof Download PDF

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CN116333750A
CN116333750A CN202211725193.8A CN202211725193A CN116333750A CN 116333750 A CN116333750 A CN 116333750A CN 202211725193 A CN202211725193 A CN 202211725193A CN 116333750 A CN116333750 A CN 116333750A
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algae
nitrogen
saline
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soil
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霍书豪
丁胜杰
朱菲菲
潘纲
贺朝文
胡新娟
崔溢
刘伟
穆罕默德
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Jiangsu University
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Abstract

The invention belongs to the technical field of agricultural biological fertilizers, and particularly relates to a nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil and a preparation method thereof. The modifier comprises, by weight, 10-120 parts of nitrogen fixation blue algae and 2-30 parts of auxiliary agents; the nitrogen-fixing blue algae comprise small Shan Qi algae and nitrogen-fixing anabaena; the nitrogen-fixing blue algae is algae liquid or algae powder, the auxiliary agent is phosphate-dissolving bacteria and phosphate-accumulating bacteria, or the auxiliary agent is filler and adhesive. The active biological modifier is prepared based on nitrogen fixation blue algae optimization and recombination, and can be used for improving nitrogen sources and phosphorus sources in saline-alkali soil, so that the aim of improving nitrogen and phosphorus which can be absorbed by plants in the saline-alkali soil is fulfilled, the loss of nitrogen and phosphorus in the soil is reduced, the soil-land junction condition is improved, and the utilization rate of the soil is enhanced; meanwhile, the method has the advantages of green, environmental protection, economy and the like; can also be used for preparing the nitrogen-phosphorus bioactive compound fertilizer for the saline-alkali soil paddy field and rape, and meets the requirement of agricultural production on the diversification of fertilizer functions.

Description

Nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil and preparation method thereof
Technical Field
The invention belongs to the technical field of agricultural biological fertilizers, and particularly relates to a nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil and a preparation method thereof.
Background
When the salt content in the soil is greater than the normal growth level of the plant, it is called saline-alkali soil. When the saline-alkali content exceeds 0.6%, it is called heavy saline-alkali soil. The data show that the world saline-alkali soil area is 124.95 hundred million mu, and 15 hundred million mu of saline-alkali soil exists in China, wherein 8653 ten thousand mu of saline-alkali soil is heavy saline-alkali soil, and the total area of the saline-alkali soil is more than 20%; the damage of soil salinization is very large, the natural growth of trees and the agricultural productivity are adversely affected, and the ecological benefit is drastically reduced. Therefore, how to effectively and practically improve the heavy saline-alkali soil is imperative.
The large-scale long-term use of chemical fertilizers and pesticides causes pollution to soil, water and agricultural products, and simultaneously causes killing of microorganisms which depend on plant growth in the soil, thus causing imbalance of ecological balance of farmlands in high-yield areas of a plurality of crops, increasing control of plant diseases and insect pests, increasing serious problems of farmland pollution, water pollution, agricultural product quality reduction and the like, and also causing irreversible influence on the environment. The influence of the microbial fertilizer on the soil and fruit quality of the kiwi fruit orchard is researched through experiments, and although the microbial fertilizer has a certain improvement effect, the microbial fertilizer is a single flora and has a slower improvement speed, and part of strains have an inhibition effect on the soil property.
The active microalgae fertilizer can improve the microbial ecosystem of soil, improve fertility, reduce fertilizer use and help crops to maximally absorb and utilize nutrient substances. The microalgae has strong ammonia nitrogen utilization capability, can utilize ammonia nitrogen in vivo to synthesize amino acid without consuming energy, and the amino acid is absorbed by root systems and enters plants to synthesize protein. Meanwhile, secretion of microalgae extracellular matters can help growth of soil bacteria.
About 30% of farmlands worldwide are deficient in phosphorus. Saline-alkali soil crop productivity increases more depending on phosphorus than other nutrient elements or environmental factors. The importance of enhancing the recycling of phosphorus is self-evident. However, excessive application of conventional phosphate fertilizers can also adversely affect the soil, including in particular (1) excessive phosphorus nutrition can promote a significant increase in respiration of the crop, thereby consuming sugar and energy stored in the crop. (2) After excessive application of the superphosphate, insoluble zinc phosphate salt can be formed in a large amount in soil, so that obvious zinc deficiency symptoms appear in crops; after the excessive application of alkaline phosphate fertilizers such as calcium, magnesium, phosphorus and the like, soil can be alkalized, so that the effectiveness of zinc is reduced, and the absorption of zinc by crops is affected. (3) excessive phosphorus application can cause the crops to lose silicon. Silicon has a very positive effect on the growth and high yield of many crops. Among them, the rice silicon deficiency is sometimes caused by a large amount of phosphorus application. (4) When the phosphate fertilizer is excessively applied, the phenomenon of strong phosphorus and weak molybdenum often occurs between phosphorus and molybdenum, so that crops cannot absorb and utilize the phosphorus and the molybdenum. (5) Phosphate fertilizers are mainly derived from phosphate ores, which contain a plurality of impurities. Including cadmium, lead, fluorine and other harmful elements. Thus, excessive application of phosphate fertilizer can cause the accumulation of harmful elements in the soil.
Disclosure of Invention
According to the problems existing in the prior art at present, the invention aims to provide a preparation method of a nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil, and the fertilizer can effectively fix nitrogen and capture phosphorus, so that nitrogen and phosphorus in the soil are improved, and nutrient absorption of crops is promoted.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the nitrogen fixation algae floc active phosphorus biological soil conditioner for the saline-alkali soil comprises, by weight, nitrogen fixation blue algae and auxiliary agents, wherein the nitrogen fixation blue algae is 10-120 parts, and the auxiliary agents are 2-30 parts; the nitrogen-fixing blue algae comprise small Shan Qi algae and nitrogen-fixing anabaena;
the nitrogen fixation blue algae is algae liquid or algae powder; the auxiliary agent is divided into a first auxiliary agent and a second auxiliary agent; the first auxiliary agent is phosphate solubilizing bacteria and phosphate accumulating bacteria; the second auxiliary agent is filler and adhesive.
1. Liquid preparation:
(1) When the soil conditioner is a liquid preparation, the nitrogen-fixing blue algae is a small Shan Qi algae culture solution and a nitrogen-fixing anabaena culture solution; the auxiliary agent is selected from a first auxiliary agent which is phosphate-solubilizing bacteria and phosphate-accumulating bacteria; the small Shan Qi algae culture solution and the nitrogen-fixing anabaena culture solution are algae solutions cultured to a logarithmic phase, and the concentrations of the small Shan Qi algae culture solution and the nitrogen-fixing anabaena culture solution are 0.3 g/L-0.5 g/L;
according to the weight parts, the algae culture solution of Shan Qi is 10-20 parts, the nitrogen fixation anabaena culture solution is 10-20 parts, the phosphate solubilizing bacteria is 1-10 parts, and the phosphate accumulating bacteria is 1-5 parts;
further, the phosphate solubilizing bacteria comprise one or more of bacillus megaterium (Bacillus megateriumde), bacillus mucilaginosus (Bacillus mucilaginosus) and bacillus azotemmaus (Bacillus azotoformans).
Wherein, the bacillus megatherium can degrade indissolvable phosphorus-containing compounds in soil, has nitrogen fixation and synergism when being mixed with bacillus sphaericus (Bacillus sphaericus) for culture, can improve soil fertility, and increases yield and income; bacillus mucilaginosus can promote the transformation of ineffective phosphorus and potassium in soil, increase the supply of phosphorus and potassium in soil, improve the crop yield, and also can produce carbonic anhydrase, thereby having a certain effect on the fixation of carbon dioxide. The nitrogen fixing bacillus has strong nitrogen fixing capability and strong competitive adaptability.
Further, the small Shan Qizao (Tolypothrix tenuis): the abnormal cells contain rich nitrogen fixation enzymes, and are places for fixing nitrogen of blue algae; anabaena (Anabaena azotica): besides rich nitrogen fixation enzymes, the main thing is that the nitrogen fixation enzymes can dissolve phosphorus in soil, increase effective phosphorus in soil and reduce the need of plants for additional phosphorus.
Further, the phosphorus accumulating bacteria include one or more of Acinetobacter sp, aeromonas sp and Pseudomonas sp.
Wherein the Acinetobacter can form polyphosphate in cells, and then can degrade the polyphosphate in the cells to release phosphorus under the conditions of vigorous metabolism and anaerobism; aeromonas can form polyphosphate inclusions and degrade organic matter; the polyphosphate content of Pseudomonas from logarithmic growth phase to stationary growth phase increases with the increase of the cultivation time under aerobic conditions.
Further, the culture solution of the small Shan Qi algae and the culture solution of the nitrogen-fixing anabaena are both culture solutions which are cultured to the logarithmic phase, and the culture mediums are both BG11-N culture mediums; the culture time of the small Shan Qi algae is 10-14 days, and the culture time of the nitrogen-fixing anabaena is 7-12 days; the culture conditions are as follows: 120r/min, maintaining the temperature at 25deg.C, and light intensity at 2500lux, and illuminating in the whole course.
(2) The invention provides a preparation method of the nitrogen fixation algae floc active phosphorus biological soil conditioner liquid preparation for saline-alkali soil, which comprises the following steps:
mixing the small Shan Qi algae culture solution and the nitrogen-fixing anabaena culture solution to obtain a mixed blue algae solution, adjusting the pH, and then adding phosphate-dissolving bacteria and phosphate-accumulating bacteria to obtain the nitrogen-fixing algae floc active phosphorus biological soil conditioner for the saline-alkali soil.
Further, the pH of the mixed blue algae solution is adjusted to 7-7.5.
2. Solid preparation:
(1) When the soil conditioner is a solid preparation, the nitrogen-fixing blue algae is algae powder of the chlorella Shan Qi and the nitrogen-fixing anabaena; the auxiliary agent is selected from a second auxiliary agent which is a filler and an adhesive;
according to the weight parts, the algae powder of the bifidobacterium is 20-40 parts, the algae powder of the nitrogen fixation anabaena is 50-80 parts, the filler is 10-20 parts, and the adhesive is 5-10 parts;
further, the filler is one or more of bentonite, diatomite, vermiculite and kaolin.
Further, the algae powder of the nitrogen-fixing blue algae is nitrogen-fixing Anabaena (Anabaena azotica) and small Shan Qizao (Tolypothrix tenuis).
Further, the adhesive is one or more of hydroxymethyl cellulose, sodium hydroxymethyl cellulose, methyl cellulose, ethyl cellulose and gum arabic.
Further, the algae used by the algae powder of the small bifidobacterium and the algae powder of the nitrogen-fixing anabaena are the small Shan Qi algae and the nitrogen-fixing anabaena which are cultivated to the logarithmic phase; the culture mediums are BG11-N culture mediums; the culture time of the small Shan Qi algae is 10-14 days, and the culture time of the nitrogen-fixing anabaena is 7-12 days; the culture conditions are as follows: 120r/min, maintaining the temperature at 25deg.C, and light intensity at 2500lux, and illuminating in the whole course.
(2) The invention provides a preparation method of an active phosphorus biological soil conditioner for nitrogen fixation algae flocs in saline-alkali soil, which comprises the following steps:
respectively grinding the dried nitrogen-fixing anabaena and the small Shan Qizao to obtain powder, putting the powder into a coating machine, then adding an adhesive and a filler, adding the adhesive according to 15-20% of the weight of the filler, and uniformly coating the filler on the algae powder to obtain pelleted algae powder, namely the nitrogen-fixing algae floc active phosphorus biological soil conditioner for saline-alkali soil.
Application: the nitrogen fixation algae floc active phosphorus biological soil conditioner prepared by the invention is applied to the saline-alkali soil to promote nitrogen sources and phosphorus sources.
Further, the growth cycle of the rice in the rice planting process comprises a seedling stage, a turning green stage, a tillering stage, a spike stage and a maturing stage, wherein the phosphorus-catching nitrogen-fixing double-effect algae floc active biological fertilizer needs to be added in one of the following stages.
After planting, the blue algae does not need to be harvested, dies naturally and is released into the soil, and the organic matter content of the soil can be increased.
Compared with the traditional fertilizer, the application has at least the following effective effects:
(1) The added nitrogen-fixing anabaena has rich nitrogen-fixing enzyme capable of fixing nitrogen in atmosphere to synthesize amino acid and protein, so as to increase the requirement of plant on nitrogen, and the nitrogen-fixing principle is N 2 +8e - +16Mg ATP+8H + →2NH 3 +H 2 +16Mg ADP+16P i . It can also dissolve phosphorus in soil, increase effective phosphorus in soil, reduce the need of plant for additional phosphorus, and its principle of dissolving phosphorus is Ca 3 (PO 4 ) 2 +C n O n H＝H + +2PO 4 3- +C n O n - +3Ca 2+ . In addition, the Extracellular Polymer (EPS) with abundant polysaccharide as the main component can adsorb heavy metals in soil, thereby achieving the purposes of improving the physical and chemical properties of the soil and improving the fertilizer and water retention capacity of the soil.
(2) The added small Shan Qizao belongs to the filamentous blue algae and contains special metamorphosis nutrition cells-dysmorphism cells. The abnormal cells contain rich nitrogen fixation enzymes, and are places for fixing nitrogen of blue algae; the special-shaped cells must form a coating layer on the original cell wall in the differentiation forming process to prevent oxygen from entering, so as to ensure that a micro-oxygen environment is formed inside the special-shaped cells, and protect the nitrogen fixation enzyme from being deactivated, thereby being capable of directly absorbing nitrogen in the atmosphere in an oxygen-enriched environment and reducing the nitrogen into NH 4 + 。
Therefore, the preparation method of the saline-alkali soil nitrogen fixation algae floc active phosphorus biological soil conditioner provided by the application can improve soil fertility (nitrogen source and phosphorus source), increase the organic matter content of the soil, and reduce the dependence on traditional fertilizers and pollution caused by the dependence; provides an effective method for improving the yield and quality of grains (rice).
Drawings
FIG. 1 is a graph comparing soil phosphorus available under different treatment groups.
FIG. 2 is a graph comparing soil ammonium nitrogen under different treatment groups.
FIG. 3 is a graph comparing soil inorganic phosphorus morphology under different treatment groups, wherein (a) is Al-P; (b) is Fe-P; (c) is Ca-P.
FIG. 4 is a graph comparing soil organophosphorus morphology in different treatment groups, wherein (a) is active organophosphorus, (b) is moderately active organophosphorus, (c) is moderately stable organophosphorus, and (d) is highly stable organophosphorus.
Detailed Description
The features and specific objects and functions of the present invention will be further described by way of examples, with the understanding that the present invention is applicable to both the present invention and the following detailed description. It should be noted that the following examples and drawings only illustrate the present invention in further detail, but do not limit the scope of the present invention to the examples. Any simple modification, similar modification, or similar substitution without resorting to inventive faculty by those skilled in the art, without departing from the spirit and principles of this invention, shall fall within the scope of this invention.
In the following implementation examples:
small Shan Qizao (FACHB-185) and nitrogen-fixing anabaena (FACHB-119) were purchased from the fresh water algae seed stock of the national academy of sciences; the phosphate-solubilizing bacteria (bacillus megaterium ACCC 04314) and the phosphate-accumulating bacteria (acinetobacter ACCC 60234) are purchased from China center for type culture collection.
The BG11-N culture medium comprises citric acid 6mg/L and ferric ammonium citrate 6mg/L, caCl 2 ·2H 2 O 36mg/L、K 2 HPO 4 ·3H 2 O 40mg/L、MgSO 4 ·7H 2 O 75mg/L、Na 2 CO 3 20mg/L、Na 2 EDTA·2H 2 O1 mg/L, trace metal solution 1mL/L (H) 3 BO 3 2.86g/L、MnCl 2 ·4H 2 O 1.86g/L、ZnSO 4 ·7H 2 O 0.22g/L、Na 2 MoO 4 .2H 2 O0.39g/L、CuSO 4 .5H 2 O 0.08g/L、Co(NO 3 ) 2 .6H 2 O0.05 g/L) and pH was adjusted to 7.1 with 1mol/L NaOH or HCl solution.
Example 1:
(1) Activating small Shan Qizao by inoculating the Bifidobacterium strain purchased from the fresh water algae strain library of China academy of sciences into 100mL triangular flask containing 50mL of liquid BG11-N medium under aseptic condition; placing on a shaking table, shaking at 120r/min, maintaining the temperature at 25deg.C, and illuminating with 2500lux light. Measuring the dry weight of the strain to judge the concentration change, culturing for 14 days, growing to a logarithmic growth phase, inoculating the strain to a 500mL triangular flask containing 250mLBG11-N culture medium according to the concentration of 0.3g/L, continuously placing the strain on a shaking table to shake at the speed of 120r/min, maintaining the temperature at 25 ℃, the light intensity at 2500lux, and continuously culturing for 14 days to the logarithmic growth phase under the whole-course illumination to obtain activated small Shan Qizao;
(2) Culturing by column aeration of small Shan Qizao, namely transferring activated Bifidobacterium oxydans into column tube containing 500mL BG11-N culture medium for amplification culture, wherein the inoculation concentration is 0.3g/L; culturing in an illumination incubator for 10 days at 25deg.C with illumination setting 2500lux and air ventilation of 0.6L/min to obtain a culture solution of small Shan Qi algae with concentration of 0.3g/L for final experiment;
(3) Activating nitrogen-fixing anabaena, inoculating nitrogen-fixing anabaena purchased from a fresh water algae seed warehouse of the academy of sciences of China into a 100mL triangular flask containing 50mL of liquid BG11-N culture medium, placing the triangular flask on a shaking table to shake at a speed of 120r/min, keeping the temperature at 25 ℃, and carrying out light intensity 2500lux and whole-course illumination; detecting biomass increase by a dry weight measurement method, culturing for 12 days, inoculating the anabaena with nitrogen fixation to a 500mL triangular flask containing 250mL of BG11-N culture medium according to the concentration of 0.4g/L when the anabaena grows to the logarithmic growth phase, maintaining the temperature at 25 ℃, and obtaining the activated anabaena with nitrogen fixation by illumination in the whole process and continuous culture for 12 days under the light intensity of 2500 lux;
(4) The nitrogen-fixing anabaena column type aeration culture, namely inoculating the nitrogen-fixing anabaena activated in the step (3) into a column tube containing 500mL of BG11-N culture medium for expansion culture, placing the column tube into an illumination incubator for 7 days, setting illumination to 2500lux and air ventilation to 0.6L/min, and obtaining nitrogen-fixing anabaena culture solution which is required to grow to a logarithmic phase in a final experiment, wherein the concentration is 0.4g/L;
the invention provides an active phosphorus biological soil conditioner for nitrogen fixation algae flocs in saline-alkali soil, which comprises the following raw materials, by weight, 10 parts of bacillus megatherium, 20 parts of nitrogen fixation anabaena culture solution, 20 parts of small Shan Qi algae culture solution and 5 parts of acinetobacter.
(5) A method for preparing a liquid preparation of an active phosphorus biological soil conditioner for fixing nitrogen algae flocs in saline-alkali soil comprises the following steps of mixing a small Shan Qi algae culture solution and a nitrogen-fixing anabaena culture solution to obtain a mixed blue algae solution, adjusting the pH to 7, and then adding bacillus megaterium and Acinetobacter to obtain the active phosphorus-catching and nitrogen-fixing double-effect algae flocs liquid conditioner.
Setting a comparison experiment: A. distilled water is applied; B. applying BG11-N medium; C. applying a liquid modifying agent;
and (3) detecting soil properties, namely respectively placing the rice field surface layer with the same mass in a glass square jar to air-dry the soil, and then respectively applying distilled water, BG11-N culture medium and liquid modifier according to 25% of the soil mass. A certain amount of distilled water was applied every 3 days.
The soil was examined for inorganic phosphorus, organic phosphorus, available phosphorus, ammonium nitrogen every 5 days from the day of the start of the experiment. The detection method can be referred to the method for analyzing soil agricultural chemistry of the main code Lu Rukun.
Analysis of results: as can be seen from FIGS. 1-2, the application of the liquid modifier increases the effective phosphorus and ammonium nitrogen content of the soil as compared to the application of distilled water and BG11-N medium. Among them, it can be seen from FIG. 3 that the contents of Al-P and Fe-P are mainly increased in the form of inorganic phosphorus, and Fe-P is a main inorganic phosphorus component in the content, which is probably because of the climatic reasons of the present study test site and the weakly acidic test soil resulting in a higher content of Fe-Al ions in the soil. After the phosphate fertilizer is applied into the soil, the phosphate fertilizer is firstly converted into Fe-P and Al-P, wherein part of Al-P is also continuously converted into Fe-P. The Ca-P content increases slowly, probably because plant rhizomes remaining in the soil directly absorb part of Ca-P. As can be seen from fig. 4, for the organophosphorus component, the application of phosphorus increases the organophosphorus content of each component, mainly by increasing the active organophosphorus and the medium active organophosphorus content, mainly because the application of phosphorus increases the activity of soil microorganisms and thus increases their immobilization and thus increases the organophosphorus content.
Example 2:
respectively activating and culturing the nitrogen-fixing anabaena and the small Shan Qi algae according to the operations of the steps (1) - (4) of the example 1 to obtain a small Shan Qi algae culture solution with the concentration of 0.3g/L and a nitrogen-fixing anabaena culture solution with the concentration of 0.4g/L; then taking out the nitrogen-fixing anabaena and the small Shan Qizao, naturally air-drying and grinding into powder to obtain the algae powder of the nitrogen-fixing anabaena and the algae powder of the small bifidobacterium;
the invention provides an active phosphorus biological soil conditioner of nitrogen fixation algae flocs in saline-alkali soil, which comprises the following raw materials, by weight, 80 parts of algae powder of nitrogen fixation anabaena, 30 parts of algae powder of small bifidobacterium, 10 parts of kaolin and 5 parts of adhesive hydroxymethyl cellulose.
A preparation method of solid pelleting algae powder of nitrogen fixation algae floc active phosphorus biological soil conditioner in saline-alkali soil comprises the following steps: the algae powder of the nitrogen-fixing anabaena and the algae powder of the small bifidobacterium are put into a coating machine, the algae powder and the small anabaena are bonded together by adding adhesive hydroxymethyl cellulose, filler kaolin is continuously added at a constant speed, and an adhesive is added according to 20 percent of the weight of the filler, so that the filler is uniformly coated on the algae powder, and the pelleted algae powder is obtained.
Three sets of experiments were set up: A. distilled water is applied; B. applying BG11-N medium; C. the pelleted algae powder is applied.
And (3) detecting soil properties, namely respectively placing the rice field surface layer air-dried soil with the same mass in a glass square jar, and then applying the following components according to 25% of the soil mass: A. distilled water; B. BG11-N medium; C. the algae powder is pelleted.
Detecting inorganic phosphorus form, organic phosphorus form, available phosphorus and ammonium nitrogen of soil every 5 days from the beginning of the experiment; the detection method is described in the main code of Lu Rukun, namely "soil agrochemical analysis method".
Analysis of results: compared with the application of distilled water and BG11-N culture medium, the influence of the application of the pelleted algae powder on the nitrogen and phosphorus of the soil is consistent with the trend of the application of the liquid modifier in the embodiment 1, so that the nitrogen and phosphorus content of the soil is obviously improved; however, the content of the dry algae powder is slightly lower than that of the liquid modifier, and the reason is probably that the dry algae powder can not be activated until the dry algae powder absorbs water, so that the nitrogen and phosphorus content of the soil can be increased.
Description: the above embodiments are only for illustrating the present invention and not for limiting the technical solution described in the present invention; thus, while the invention has been described in detail with reference to the various embodiments described above, it will be understood by those skilled in the art that the invention may be modified or equivalents; all technical solutions and modifications thereof that do not depart from the spirit and scope of the present invention are intended to be included in the scope of the appended claims.

Claims (10)

1. The nitrogen fixation algae floc active phosphorus biological soil conditioner for the saline-alkali soil is characterized by comprising, by weight, 10-120 parts of nitrogen fixation algae and 2-30 parts of auxiliary agents; the nitrogen-fixing blue algae comprise small Shan Qi algae and nitrogen-fixing anabaena; the nitrogen fixation blue algae is algae liquid or algae powder; the auxiliary agent is a first auxiliary agent or a second auxiliary agent; the first auxiliary agent is phosphate solubilizing bacteria and phosphate accumulating bacteria; the second auxiliary agent is filler and adhesive.
2. The nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil according to claim 1, wherein when the nitrogen fixation algae is algae liquid, a first auxiliary agent is selected; the nitrogen-fixing blue algae is a small Shan Qi algae culture solution and a nitrogen-fixing anabaena culture solution, and the concentrations of the small Shan Qi algae culture solution and the nitrogen-fixing anabaena culture solution are 0.3 g/L-0.5 g/L;
according to the weight parts, the algae culture solution of Shan Qi is 10-20 parts, the nitrogen fixation anabaena culture solution is 10-20 parts, the phosphate solubilizing bacteria is 1-10 parts, and the phosphate accumulating bacteria is 1-5 parts;
the phosphate solubilizing bacteria comprise one or more of bacillus megatherium, bacillus mucilaginosus and bacillus azotems; the phosphorus accumulating bacteria comprise one or more of Acinetobacter genus, aeromonas genus and pseudomonas genus.
3. The nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil, according to claim 2, wherein the small Shan Qi algae culture solution and the nitrogen fixation anabaena culture solution are culture solutions which are cultivated to a logarithmic phase, and the culture mediums are BG11-N culture mediums; the culture time of the small Shan Qi algae is 10-14 days, and the culture time of the nitrogen-fixing anabaena is 7-12 days; the culture conditions are as follows: 120r/min, maintaining the temperature at 25deg.C, and light intensity at 2500lux, and illuminating in the whole course.
4. The nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil according to claim 1, wherein when the nitrogen fixation algae is algae powder, the second auxiliary agent is selected; the nitrogen-fixing blue algae is algae powder of small Shan Qi algae and nitrogen-fixing anabaena;
the weight portions of the components are 20-40 portions of the algae powder of the small bifidobacterium, 50-80 portions of the algae powder of the nitrogen fixation anabaena, 10-20 portions of the filler and 5-10 portions of the adhesive.
5. The nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil according to claim 4, wherein the filler is one or more of bentonite, diatomite, vermiculite and kaolin; the adhesive is one or more of hydroxymethyl cellulose, sodium hydroxymethyl cellulose, methyl cellulose, ethyl cellulose and gum arabic.
6. The nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil according to claim 4, wherein algae powder of the small bifidobacterium and algae powder of the nitrogen fixation anabaena are small Shan Qi algae and nitrogen fixation anabaena which are cultivated to a logarithmic phase; the culture mediums are BG11-N culture mediums; the culture time of the small Shan Qi algae is 10-14 days, and the culture time of the nitrogen-fixing anabaena is 7-12 days; the culture conditions are as follows: 120r/min, maintaining the temperature at 25deg.C, and light intensity at 2500lux, and illuminating in the whole course.
7. The preparation method of the nitrogen fixation algae floc active phosphorus biological soil conditioner for the saline-alkali soil according to claim 2, wherein the mixed blue algae solution is obtained by mixing a small Shan Qi algae culture solution and a nitrogen fixation anabaena culture solution, and after adjusting the pH, the phosphorus-dissolving bacteria and the phosphorus-accumulating bacteria are added to obtain the nitrogen fixation algae floc active phosphorus biological soil conditioner for the saline-alkali soil.
8. The method for preparing the nitrogen fixation algae floc active phosphorus biological soil conditioner for the saline-alkali soil according to claim 7, wherein the pH of the mixed blue algae solution is adjusted to 7-7.5.
9. The method for preparing the nitrogen-fixing algae floc active phosphorus biological soil conditioner for the saline-alkali soil according to claim 4, wherein the dried nitrogen-fixing algae floc active phosphorus biological soil conditioner for the saline-alkali soil is prepared by respectively grinding dry nitrogen-fixing algae and small Shan Qizao to obtain powder, putting the powder into a coating machine, adding an adhesive and a filler, and adding an adhesive according to 15-20% of the weight of the filler to uniformly wrap the filler on the algae powder, thereby obtaining the pelleted algae powder, namely the nitrogen-fixing algae floc active phosphorus biological soil conditioner for the saline-alkali soil.
10. Use of the nitrogen fixation algae floc active phosphorus biological soil conditioner according to any one of claims 1-6 in saline-alkali soil to promote nitrogen sources and phosphorus sources.
CN202211725193.8A 2022-12-30 2022-12-30 Nitrogen fixation algae floc active phosphorus biological soil conditioner for saline-alkali soil and preparation method thereof Pending CN116333750A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117865755A (en) * 2024-03-12 2024-04-12 内蒙古阿尔格生命科学有限公司 Saline-alkali soil modifier and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117865755A (en) * 2024-03-12 2024-04-12 内蒙古阿尔格生命科学有限公司 Saline-alkali soil modifier and preparation method thereof
CN117865755B (en) * 2024-03-12 2024-05-14 内蒙古阿尔格生命科学有限公司 Saline-alkali soil modifier and preparation method thereof

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