CN117865755B - Saline-alkali soil modifier and preparation method thereof - Google Patents
Saline-alkali soil modifier and preparation method thereof Download PDFInfo
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- CN117865755B CN117865755B CN202410280698.0A CN202410280698A CN117865755B CN 117865755 B CN117865755 B CN 117865755B CN 202410280698 A CN202410280698 A CN 202410280698A CN 117865755 B CN117865755 B CN 117865755B
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- saline
- alkali soil
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- oyster shell
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- 239000002689 soil Substances 0.000 title claims abstract description 126
- 239000003513 alkali Substances 0.000 title claims abstract description 93
- 238000002360 preparation method Methods 0.000 title claims abstract description 57
- 239000003607 modifier Substances 0.000 title claims abstract description 52
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 70
- 238000002156 mixing Methods 0.000 claims abstract description 50
- 239000002131 composite material Substances 0.000 claims abstract description 42
- 239000011159 matrix material Substances 0.000 claims abstract description 29
- 238000007873 sieving Methods 0.000 claims abstract description 25
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000003124 biologic agent Substances 0.000 claims abstract description 18
- 239000011591 potassium Substances 0.000 claims abstract description 18
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 18
- 239000010440 gypsum Substances 0.000 claims abstract description 17
- 229910052602 gypsum Inorganic materials 0.000 claims abstract description 17
- 241000609240 Ambelania acida Species 0.000 claims abstract description 16
- 244000060011 Cocos nucifera Species 0.000 claims abstract description 16
- 235000013162 Cocos nucifera Nutrition 0.000 claims abstract description 16
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims abstract description 16
- 239000010905 bagasse Substances 0.000 claims abstract description 16
- 235000019837 monoammonium phosphate Nutrition 0.000 claims abstract description 16
- 239000006012 monoammonium phosphate Substances 0.000 claims abstract description 16
- 238000000227 grinding Methods 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 239000010903 husk Substances 0.000 claims abstract description 3
- 241000192542 Anabaena Species 0.000 claims description 59
- 238000003756 stirring Methods 0.000 claims description 55
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 53
- 238000005286 illumination Methods 0.000 claims description 44
- 229920002261 Corn starch Polymers 0.000 claims description 42
- 239000008120 corn starch Substances 0.000 claims description 42
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 41
- 239000012265 solid product Substances 0.000 claims description 41
- 239000007788 liquid Substances 0.000 claims description 39
- 239000012263 liquid product Substances 0.000 claims description 37
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 36
- 239000008367 deionised water Substances 0.000 claims description 34
- 229910021641 deionized water Inorganic materials 0.000 claims description 34
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 28
- 238000012258 culturing Methods 0.000 claims description 28
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 26
- 239000012881 co-culture medium Substances 0.000 claims description 26
- 239000000843 powder Substances 0.000 claims description 26
- 238000005406 washing Methods 0.000 claims description 25
- 238000001035 drying Methods 0.000 claims description 24
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 24
- 241000194107 Bacillus megaterium Species 0.000 claims description 23
- 238000001694 spray drying Methods 0.000 claims description 23
- 241000237502 Ostreidae Species 0.000 claims description 22
- 235000020636 oyster Nutrition 0.000 claims description 22
- 239000011248 coating agent Substances 0.000 claims description 19
- 238000000576 coating method Methods 0.000 claims description 19
- 239000000126 substance Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 16
- 235000011187 glycerol Nutrition 0.000 claims description 14
- 229910052757 nitrogen Inorganic materials 0.000 claims description 14
- 239000000725 suspension Substances 0.000 claims description 14
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 13
- 230000004913 activation Effects 0.000 claims description 13
- 229960002713 calcium chloride Drugs 0.000 claims description 13
- 239000001110 calcium chloride Substances 0.000 claims description 13
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 13
- 238000003501 co-culture Methods 0.000 claims description 13
- 238000002791 soaking Methods 0.000 claims description 13
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 12
- JYLNVJYYQQXNEK-UHFFFAOYSA-N 3-amino-2-(4-chlorophenyl)-1-propanesulfonic acid Chemical compound OS(=O)(=O)CC(CN)C1=CC=C(Cl)C=C1 JYLNVJYYQQXNEK-UHFFFAOYSA-N 0.000 claims description 12
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 12
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 12
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 claims description 12
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 12
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 12
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 12
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 claims description 12
- 229940052299 calcium chloride dihydrate Drugs 0.000 claims description 12
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 12
- 229960004642 ferric ammonium citrate Drugs 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000004021 humic acid Substances 0.000 claims description 12
- 239000004313 iron ammonium citrate Substances 0.000 claims description 12
- 235000000011 iron ammonium citrate Nutrition 0.000 claims description 12
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 12
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 12
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 12
- AWNVVAMWLMUZOZ-UHFFFAOYSA-J magnesium;disodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Mg+2].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O AWNVVAMWLMUZOZ-UHFFFAOYSA-J 0.000 claims description 12
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 claims description 12
- 230000007935 neutral effect Effects 0.000 claims description 12
- 229910000160 potassium phosphate Inorganic materials 0.000 claims description 12
- 235000011009 potassium phosphates Nutrition 0.000 claims description 12
- 239000000661 sodium alginate Substances 0.000 claims description 12
- 235000010413 sodium alginate Nutrition 0.000 claims description 12
- 229940005550 sodium alginate Drugs 0.000 claims description 12
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 12
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 claims description 12
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 11
- 240000007817 Olea europaea Species 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 10
- 239000012190 activator Substances 0.000 claims description 2
- 239000003516 soil conditioner Substances 0.000 claims 4
- 230000000694 effects Effects 0.000 abstract description 18
- 235000015097 nutrients Nutrition 0.000 abstract description 12
- 230000000813 microbial effect Effects 0.000 abstract description 5
- 230000001105 regulatory effect Effects 0.000 abstract description 4
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 230000006870 function Effects 0.000 abstract description 2
- 230000001276 controlling effect Effects 0.000 description 50
- 238000011081 inoculation Methods 0.000 description 37
- 238000012360 testing method Methods 0.000 description 34
- 241000186660 Lactobacillus Species 0.000 description 31
- 229940039696 lactobacillus Drugs 0.000 description 31
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 29
- 241000193830 Bacillus <bacterium> Species 0.000 description 27
- 239000001963 growth medium Substances 0.000 description 20
- 239000006228 supernatant Substances 0.000 description 20
- 241000196324 Embryophyta Species 0.000 description 17
- 244000005700 microbiome Species 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 14
- 238000009631 Broth culture Methods 0.000 description 10
- 241000195493 Cryptophyta Species 0.000 description 10
- 241000208818 Helianthus Species 0.000 description 10
- 238000001914 filtration Methods 0.000 description 10
- 230000012010 growth Effects 0.000 description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 239000002245 particle Substances 0.000 description 10
- 238000004321 preservation Methods 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 235000003222 Helianthus annuus Nutrition 0.000 description 9
- 239000012299 nitrogen atmosphere Substances 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 9
- 238000004659 sterilization and disinfection Methods 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 8
- 238000004806 packaging method and process Methods 0.000 description 8
- 150000003839 salts Chemical class 0.000 description 8
- 241000235342 Saccharomycetes Species 0.000 description 7
- 230000009286 beneficial effect Effects 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000004310 lactic acid Substances 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 230000007547 defect Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 4
- 238000003973 irrigation Methods 0.000 description 4
- 230000002262 irrigation Effects 0.000 description 4
- 230000035699 permeability Effects 0.000 description 4
- 230000008439 repair process Effects 0.000 description 4
- 230000035558 fertility Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000005067 remediation Methods 0.000 description 3
- 239000002344 surface layer Substances 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 239000003673 groundwater Substances 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000051 modifying effect Effects 0.000 description 2
- 235000015816 nutrient absorption Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- FZUJWWOKDIGOKH-UHFFFAOYSA-N sulfuric acid hydrochloride Chemical compound Cl.OS(O)(=O)=O FZUJWWOKDIGOKH-UHFFFAOYSA-N 0.000 description 2
- 240000002900 Arthrospira platensis Species 0.000 description 1
- 235000016425 Arthrospira platensis Nutrition 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000193417 Brevibacillus laterosporus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000222175 Diutina rugosa Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Plant Pathology (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The application relates to the technical field of soil treatment, and particularly discloses a saline-alkali soil modifier and a preparation method thereof. The saline-alkali soil modifier is prepared by mixing, grinding and sieving 20-30 parts of desulfurized gypsum, 40-50 parts of coconut husk and 40-50 parts of bagasse, adding 8-12 parts of a composite biological agent, 15-25 parts of oyster shell matrix, 1-3 parts of monoammonium phosphate and 0.5-2 parts of potassium humate, and uniformly mixing; the raw materials of the formula are selected and combined, so that the saline-alkali soil modifier product has various functions of repairing the saline-alkali soil; by adopting the formula raw materials, the proportion is strictly regulated and controlled, and the raw materials are synergistic, so that various nutrients are provided, the soil structure is improved, the microbial activity is promoted, the acidity and alkalinity of the soil are regulated, and the effect and efficiency of saline-alkali soil restoration are further improved.
Description
Technical Field
The application relates to the technical field of soil treatment, in particular to a saline-alkali soil modifier and a preparation method thereof.
Background
The saline-alkali soil is a soil which contains more salt and causes whitening of the surface layer of the soil, hardening of the soil and influence or block normal growth of plants, and has the characteristics of high salt content, high pH value, easy hardening of the surface layer, poor air permeability and water permeability, low nutrient content and the like. The formation of saline-alkali soil is usually the result of the combined action of natural conditions, including drought, high evaporation capacity, high groundwater level, etc., and human factors, including improper irrigation management, improper land utilization, poor drainage, excessive application of fertilizer, etc.
Saline-alkali soil remediation is a comprehensive process involving multiple disciplines such as soil science, environmental engineering and botanics. The existing repair technology mainly comprises a physical repair technology, a chemical repair technology, a biological repair technology and the like, and specifically comprises the following steps:
1. The physical restoration technology comprises deep ploughing, water conservancy drainage and the like, wherein the deep ploughing is to reduce the salt content in surface soil through deep ploughing of soil; the method is simple and easy to implement, has obvious effect, can damage the soil structure, and is only suitable for the condition that the salt accumulation on the surface layer is more. The water conservancy drainage is realized by establishing an effective drainage system to accelerate the washing of salt and the lowering of underground water level; the method can fundamentally solve the problem of soil salinization, but has the defects of high construction cost, too dependence on local water resource conditions and the like.
2. The modifying agents such as gypsum, sulfate and the like are applied in the chemical restoration technology to replace sodium ions in soil, reduce the pH value of the soil, and can effectively reduce the saline-alkali degree of the soil, but the modifying action period on the saline-alkali soil is long, and the environment is negatively affected after long-term application. The method for acidizing the saline-alkali soil is to utilize acidic substances to reduce the pH value of the soil, so that the soil environment is not beneficial to accumulation of salt; the method is quick and effective, but the soil is extremely easy to be over-acidified due to improper operation, and side effects are generated on the environment.
3. Bioremediation techniques include planting saline-alkali tolerant plants, applying microbial remediation agents, and the like; the method has the advantages of being environment-friendly, capable of simultaneously realizing soil restoration and biomass energy production, but has the defects of slow restoration process, high requirement on plant variety selection and the like. The microbial remediation agent is used for improving the saline-alkali soil condition by utilizing specific microbial flora, and the method has the defects of sustainability, small influence on environment, complex technical implementation, great influence on the effect by external environmental factors and the like.
Aiming at the limitations of the saline-alkali soil restoration technology, how to seek a more effective and comprehensive restoration solution with strong restoration performance, realizing sustainable development and utilization of the saline-alkali soil, is an important challenge facing the current agriculture and environmental science fields, and based on the statement, the application provides a saline-alkali soil modifier and a preparation method thereof.
Disclosure of Invention
The application provides a saline-alkali soil modifier and a preparation method thereof, aiming at solving the problems of great technical implementation difficulty, high implementation cost and the like of the existing saline-alkali soil restoration technology.
In a first aspect, the application provides a preparation method of a saline-alkali soil improver, which adopts the following technical scheme:
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
S11, after the anabaena and the mixed bacteria are respectively pre-cultured, co-culturing in a co-culture medium, and after the culture period is finished, centrifugally collecting a solid product and a liquid product;
s12, adding deionized water into corn starch, stirring and mixing to obtain a suspension, adding a liquid product, glycerol and calcium chloride into the suspension, stirring and mixing, and adding acid liquor I to adjust the pH value to 5.7-6.8 to obtain a coating liquid;
S13, adding the solid product into the wrapping liquid, stirring and dispersing uniformly, and then spray-drying to obtain a composite biological preparation;
S2, preparing oyster shell matrixes:
S21, recovering fresh oyster shells, washing with water, drying, crushing and sieving to obtain oyster shell powder;
S22, performing heat activation treatment on oyster shell powder to obtain an activator;
s23, soaking the activated substance in acid liquor II, washing with water to be neutral, and drying to constant weight to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
mixing, grinding and sieving the desulfurized gypsum, the coconut chaff and the bagasse, and adding the composite biological agent, the oyster shell matrix, the monoammonium phosphate and the potassium humate to be uniformly mixed to obtain the saline-alkali soil modifier.
Preferably, the anabaena in the step S11 is at least one of anabaena sui, anabaena rhodochrous, anabaena azofor, anabaena bloom, lei Mo anabaena, spirulina, polytropic anabaena and anabaena like.
Preferably, the mixed bacteria in the step S11 include yeast, lactobacillus and bacillus.
Preferably, the bacillus is at least one of bacillus licheniformis, bacillus megaterium and bacillus laterosporus.
Preferably, the co-culture medium in step S11 comprises the following components: according to 1L, 20-24g of glucose, 15-18g of calcium chloride dihydrate, 9-11g of sodium carbonate, 2-5g of ferric ammonium citrate, 2-4g of sodium alginate, 1-3g of ammonium sulfate, 1-2g of magnesium sulfate heptahydrate, 1.5-2g of manganese chloride tetrahydrate, 0.5-1g of potassium phosphate, 0.5-1g of dipotassium hydrogen phosphate, 0.3-0.7g of disodium magnesium ethylenediamine tetraacetate, 0.2-0.5g of sodium permanganate, 0.1-0.3g of zinc sulfate heptahydrate and the balance of deionized water.
Preferably, the co-culture conditions in step S11 are as follows: the culture temperature is 25-33 ℃, the illumination intensity is 2000-3000 lux, the illumination period is 14h:10h (light: dark), the shaking table speed is 120-160rpm, and the culture time is 24h.
Preferably, the acid solution I in the step S12 is humic acid solution with mass concentration of 8-12%.
Preferably, the spray drying temperature in the step S13 is 30-50 ℃.
Preferably, the heat activation treatment conditions in the step S22 are as follows: the temperature is 400-500 ℃ and the time is 2-4h.
Preferably, the acid solution II in the step S23 is a citric acid solution with a mass concentration of 3-7%.
Preferably, the raw materials used in the step S3 are as follows: the composite biological agent comprises, by weight, 20-30 parts of desulfurized gypsum, 40-50 parts of coconut husk, 40-50 parts of bagasse, 8-12 parts of a composite biological agent, 15-25 parts of oyster shell matrix, 1-3 parts of monoammonium phosphate and 0.5-2 parts of potassium humate.
In a second aspect, the application also provides a saline-alkali soil modifier, which adopts the following technical scheme:
A saline-alkali soil modifier is prepared by the preparation method.
In summary, the application has the following beneficial effects:
1. according to the application, the solid product and the liquid product are obtained by pre-culturing and co-culturing the anabaena and the mixed bacteria, and the pre-culturing and co-culturing modes can ensure that the obtained composite biological preparation contains more kinds of beneficial microorganisms, thereby improving the functionality and effect of the saline-alkali soil modifier. Specifically, the beneficial microorganisms can decompose organic substances, provide nutrient elements required by plants, improve the structure and fertility of soil, and enhance the water retention capacity and air permeability of the soil; beneficial microorganisms can form symbiotic relation with plant root systems, promote the growth and development of plants, and improve the stress resistance and nutrient absorption capacity of the plants; the beneficial microorganisms can secrete antibacterial substances and compete with pathogenic microorganisms for nutrients, so that the growth of the pathogenic microorganisms is inhibited, and plant diseases of saline-alkali soil are reduced.
2. According to the application, after the nitrogen-fixing anabaena, candida rugosa, lactobacillus plantarum and bacillus megatherium are respectively pre-cultured, co-culture is carried out; by configuring the co-culture medium, on one hand, nutrients required by microorganisms can be provided, the growth and the propagation of the microorganisms are promoted, the quantity and the activity of the microorganisms are increased, and then the metabolites of the microorganisms are optimized, and the promotion effect on plant growth and saline-alkali soil restoration is increased; on the other hand, the co-culture medium can provide favorable growth conditions for target microorganisms, reduce growth competition of other microorganisms, further help maintain physiological state and stability of the microorganisms, and improve activity and functional performance of the microorganisms.
3. According to the application, corn starch solution, liquid product, glycerin and calcium chloride are mixed, pH is regulated by adding acid solution to prepare the coating solution, and the coating solution is used for coating the solid product, so that microorganisms can be protected, the survival rate of the solid product in the saline-alkali soil restoration process is increased, and the defect that the existing bioremediation effect is greatly influenced by external environmental factors is overcome; the liquid product after co-culture centrifugation contains a plurality of nutrient substances, so that additional nutrition can be provided for the solid product, the solid product is better adhered to the coating liquid, and the growth and activity of the solid product are promoted; in addition, the microorganisms in the liquid product and the microorganisms in the solid product can also interact to form a synergistic effect, so that the functionality and effect of the final saline-alkali soil improvement product are further enhanced; the addition of the glycerol and the calcium chloride in the coating liquid can promote the intermolecular crosslinking, increase the viscosity and stability of the coating liquid, and enable the coating liquid to be more easily coated on the surface of a solid product.
4. The oyster shell is rich in mineral substances and microelements, can provide nutrients required by plants, and promotes plant growth and restoration of saline-alkali soil; according to the application, oyster shell is recovered, subjected to heat activation treatment and then acid soaking is carried out to obtain oyster shell matrix, so that the solubility and absorbability of oyster shell can be obviously improved, and the nutrition supply effect of oyster shell on plants and the restoration effect of saline-alkali soil are improved.
5. The formula of the application is selected and combined to ensure that the saline-alkali soil modifier product has various functions of repairing the saline-alkali soil; the desulfurized gypsum contains rich sulfur elements and calcium elements, can neutralize the alkalinity of soil, improve the acid-base balance of the saline-alkali soil and promote the growth and development of plant root systems; the coconut chaff and the bagasse are rich in organic matters and organic acids, so that the soil structure can be improved, the water retention and air permeability of the soil can be increased, and the fertility and improvement effect of the saline-alkali soil can be improved; the monoammonium phosphate can provide nitrogen and phosphorus elements required by plants, promote the growth and development of the plants and increase the resistance of the plants to saline-alkali stress; the potassium humate contains organic acid and potassium element, can improve soil structure, promote plant root system development and nutrient absorption, and improve plant adaptability to saline-alkali environment; by adopting the formula, the application strictly adjusts and controls the proportion, and the various raw materials have synergistic effect, so that various nutrients are provided, the soil structure is improved, the microbial activity is promoted, the acidity and alkalinity of the soil are regulated, and the effect and efficiency of saline-alkali soil restoration are further improved.
Detailed Description
The present application will be described in further detail with reference to examples.
Examples 1-5 provide a saline-alkali soil improver and a preparation method thereof.
Example 1
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
Adding 20g of glucose, 15g of calcium chloride dihydrate, 9g of sodium carbonate, 2g of ferric ammonium citrate, 2g of sodium alginate, 1g of ammonium sulfate, 1g of magnesium sulfate heptahydrate, 1.5g of manganese chloride tetrahydrate, 0.5g of potassium phosphate, 0.5g of dipotassium hydrogen phosphate, 0.3g of disodium magnesium ethylenediamine tetraacetate, 0.2g of sodium permanganate and 0.1g of zinc sulfate heptahydrate into 500ml of deionized water, stirring and dissolving, continuously adding deionized water until the total amount is 1L, and carrying out high-pressure sterilization at 121 ℃ for 15min to obtain a co-culture medium for later use;
controlling the inoculation ratio of anabaena strain to be 5:100 (g/ml), the inoculation ratio of yeast thallus to be 2:100 (g/ml), the inoculation ratio of lactobacillus thallus to be 2:100 (g/ml), the inoculation ratio of bacillus thallus to be 1:100 (g/ml), firstly inoculating anabaena strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h to 10h (light: dark), the shaking table speed to be 150rpm, inoculating the yeast thallus, the lactobacillus thallus and the bacillus thallus after shake culture for 24h, keeping the culture condition unchanged, and centrifuging for 15min under the condition of 1000rpm after continuing to co-culture for 10d, and collecting solid products and liquid products;
S12, adding deionized water at 58 ℃ into corn starch according to a feed-liquid ratio of 1:14, stirring and mixing uniformly to obtain a suspension, keeping a heat preservation stirring state, adding liquid product with the mass of 0.5 times of the corn starch, glycerol with the mass of 0.1 time of the corn starch and calcium chloride with the mass of 0.03 time of the corn starch, stirring and mixing uniformly, and adding humic acid solution with the mass concentration of 8% to adjust the pH value to 5.7 to obtain a coating liquid;
S13, adding the solid product into the wrapping liquid according to the mass ratio of 1:3, uniformly stirring and dispersing, controlling the spray drying temperature to be 30 ℃, and performing spray drying to obtain a composite biological preparation with the particle size of 40 meshes;
S2, preparing oyster shell matrixes:
S21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 40-mesh sieve to obtain oyster shell powder;
S22, placing oyster shell powder at 400 ℃ under nitrogen atmosphere, and performing heat activation treatment for 4 hours to obtain an activated product;
S23, adding citric acid solution with the mass concentration of 3% into the activated substance, soaking for 5 hours, filtering, washing to be neutral, and drying to be constant weight at the temperature of 110 ℃ to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
mixing and grinding 20 parts of desulfurized gypsum, 40 parts of coconut chaff and 40 parts of bagasse, sieving with a 10-mesh sieve, adding 8 parts of a composite biological agent, 15 parts of oyster shell matrix, 1 part of monoammonium phosphate and 0.5 part of potassium humate, uniformly mixing, and sub-packaging to obtain the saline-alkali soil modifier.
Example 2
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
Adding 20g of glucose, 15g of calcium chloride dihydrate, 9g of sodium carbonate, 2g of ferric ammonium citrate, 2g of sodium alginate, 1g of ammonium sulfate, 1g of magnesium sulfate heptahydrate, 1.5g of manganese chloride tetrahydrate, 0.5g of potassium phosphate, 0.5g of dipotassium hydrogen phosphate, 0.3g of disodium magnesium ethylenediamine tetraacetate, 0.2g of sodium permanganate and 0.1g of zinc sulfate heptahydrate into 500ml of deionized water, stirring and dissolving, continuously adding deionized water until the total amount is 1L, and carrying out high-pressure sterilization at 121 ℃ for 15min to obtain a co-culture medium for later use;
controlling the inoculation ratio of anabaena strain to be 5:100 (g/ml), the inoculation ratio of yeast thallus to be 2:100 (g/ml), the inoculation ratio of lactobacillus thallus to be 2:100 (g/ml), the inoculation ratio of bacillus thallus to be 1:100 (g/ml), firstly inoculating anabaena strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h to 10h (light: dark), the shaking table speed to be 150rpm, inoculating the yeast thallus, the lactobacillus thallus and the bacillus thallus after shake culture for 24h, keeping the culture condition unchanged, and centrifuging for 15min under the condition of 1000rpm after continuing to co-culture for 10d, and collecting solid products and liquid products;
S12, adding deionized water at 58 ℃ into corn starch according to a feed-liquid ratio of 1:14, stirring and mixing uniformly to obtain a suspension, keeping a heat preservation stirring state, adding liquid product with the mass of 0.5 times of the corn starch, glycerol with the mass of 0.1 time of the corn starch and calcium chloride with the mass of 0.03 time of the corn starch, stirring and mixing uniformly, and adding humic acid solution with the mass concentration of 8% to adjust the pH value to 5.7 to obtain a coating liquid;
S13, adding the solid product into the wrapping liquid according to the mass ratio of 1:3, uniformly stirring and dispersing, controlling the spray drying temperature to be 30 ℃, and performing spray drying to obtain a composite biological preparation with the particle size of 40 meshes;
S2, preparing oyster shell matrixes:
S21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 40-mesh sieve to obtain oyster shell powder;
S22, placing oyster shell powder at 400 ℃ under nitrogen atmosphere, and performing heat activation treatment for 4 hours to obtain an activated product;
S23, adding citric acid solution with the mass concentration of 3% into the activated substance, soaking for 5 hours, filtering, washing to be neutral, and drying to be constant weight at the temperature of 110 ℃ to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
Mixing 22 parts of desulfurized gypsum, 42 parts of coconut chaff and 42 parts of bagasse, grinding, sieving with a 10-mesh sieve, adding 9 parts of a composite biological agent, 18 parts of oyster shell matrix, 1.5 parts of monoammonium phosphate and 0.8 part of potassium humate, uniformly mixing, and subpackaging to obtain the saline-alkali soil modifier.
Example 3
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
22g of glucose, 16.5g of calcium chloride dihydrate, 10g of sodium carbonate, 3.5g of ferric ammonium citrate, 3g of sodium alginate, 2g of ammonium sulfate, 1.5g of magnesium sulfate heptahydrate, 1.8g of manganese chloride tetrahydrate, 0.8g of potassium phosphate, 0.8g of dipotassium hydrogen phosphate, 0.5g of disodium magnesium ethylene diamine tetraacetate, 0.3g of sodium permanganate and 0.2g of zinc sulfate heptahydrate are added into 500ml of deionized water to be stirred and dissolved, and then the mixture is continuously added with deionized water until the total amount is 1L, and the mixture is autoclaved at 121 ℃ for 15min to obtain a co-culture medium for standby;
controlling the inoculation ratio of anabaena strain to be 5:100 (g/ml), the inoculation ratio of yeast thallus to be 2:100 (g/ml), the inoculation ratio of lactobacillus thallus to be 2:100 (g/ml), the inoculation ratio of bacillus thallus to be 1:100 (g/ml), firstly inoculating anabaena strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h to 10h (light: dark), the shaking table speed to be 150rpm, inoculating the yeast thallus, the lactobacillus thallus and the bacillus thallus after shake culture for 24h, keeping the culture condition unchanged, and centrifuging for 15min under the condition of 1000rpm after continuing to co-culture for 10d, and collecting solid products and liquid products;
S12, adding deionized water at 63 ℃ into corn starch according to a feed-liquid ratio of 1:16, stirring and mixing uniformly to obtain a suspension, keeping a heat preservation stirring state, adding liquid product with the mass of 0.6 times of the corn starch, glycerol with the mass of 0.2 times of the corn starch and calcium chloride with the mass of 0.04 times of the corn starch, stirring and mixing uniformly, and adding humic acid solution with the mass concentration of 10% to adjust the pH value to 6.2 to obtain a coating liquid;
s13, adding the solid product into the wrapping liquid according to the mass ratio of 1:3.5, uniformly stirring and dispersing, controlling the spray drying temperature to be 40 ℃, and performing spray drying to obtain a composite biological preparation with the particle size of 50 meshes;
S2, preparing oyster shell matrixes:
s21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 50-mesh sieve to obtain oyster shell powder;
s22, placing oyster shell powder at 450 ℃ under nitrogen atmosphere, and performing heat activation treatment for 3 hours to obtain an activated product;
S23, adding citric acid solution with the mass concentration of 5% into the activated substance, soaking for 4 hours, filtering, washing to be neutral, and drying to be constant weight at the temperature of 110 ℃ to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
mixing 25 parts of desulfurized gypsum, 45 parts of coconut chaff and 45 parts of bagasse, grinding, sieving with a 20-mesh sieve, adding 10 parts of a composite biological agent, 20 parts of oyster shell matrix, 2 parts of monoammonium phosphate and 1.2 parts of potassium humate, uniformly mixing, and packaging to obtain the saline-alkali soil modifier.
Example 4
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
Taking 24g of glucose, 18g of calcium chloride dihydrate, 11g of sodium carbonate, 5g of ferric ammonium citrate, 4g of sodium alginate, 3g of ammonium sulfate, 2g of magnesium sulfate heptahydrate, 2g of manganese chloride tetrahydrate, 1g of potassium phosphate, 1g of dipotassium hydrogen phosphate, 0.7g of disodium magnesium ethylenediamine tetraacetate, 0.5g of sodium permanganate and 0.3g of zinc sulfate heptahydrate, adding into 500ml of deionized water, stirring and dissolving, continuing to add deionized water until the total amount is 1L, and carrying out high-pressure sterilization at 121 ℃ for 15min to obtain a co-culture medium for later use;
controlling the inoculation ratio of anabaena strain to be 5:100 (g/ml), the inoculation ratio of yeast thallus to be 2:100 (g/ml), the inoculation ratio of lactobacillus thallus to be 2:100 (g/ml), the inoculation ratio of bacillus thallus to be 1:100 (g/ml), firstly inoculating anabaena strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h to 10h (light: dark), the shaking table speed to be 150rpm, inoculating the yeast thallus, the lactobacillus thallus and the bacillus thallus after shake culture for 24h, keeping the culture condition unchanged, and centrifuging for 15min under the condition of 1000rpm after continuing to co-culture for 10d, and collecting solid products and liquid products;
s12, adding deionized water at the temperature of 68 ℃ into corn starch according to the feed-liquid ratio of 1:18, stirring and mixing uniformly to obtain a suspension, keeping the state of heat preservation and stirring, adding liquid product with the mass of 0.7 times of the corn starch, glycerol with the mass of 0.3 times of the corn starch and calcium chloride with the mass of 0.05 times of the corn starch, stirring and mixing uniformly, and adding humic acid solution with the mass concentration of 12% to adjust the pH value to 6.8 to obtain a coating liquid;
S13, adding the solid product into the wrapping liquid according to the mass ratio of 1:4, uniformly stirring and dispersing, controlling the spray drying temperature to be 50 ℃, and performing spray drying to obtain a composite biological preparation with the particle size of 60 meshes;
S2, preparing oyster shell matrixes:
s21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 60-mesh sieve to obtain oyster shell powder;
s22, placing oyster shell powder at a temperature of 500 ℃ under a nitrogen atmosphere, and performing heat activation treatment for 2 hours to obtain an activated product;
S23, adding citric acid solution with the mass concentration of 7% into the activated substance, soaking for 3 hours, filtering, washing to be neutral, and drying to be constant weight at the temperature of 110 ℃ to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
Mixing 28 parts of desulfurized gypsum, 48 parts of coconut chaff and 48 parts of bagasse, grinding, sieving with a 30-mesh sieve, adding 11 parts of a composite biological agent, 22 parts of oyster shell matrix, 2.5 parts of monoammonium phosphate and 1.6 parts of potassium humate, uniformly mixing, and subpackaging to obtain the saline-alkali soil modifier.
Example 5
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
Taking 24g of glucose, 18g of calcium chloride dihydrate, 11g of sodium carbonate, 5g of ferric ammonium citrate, 4g of sodium alginate, 3g of ammonium sulfate, 2g of magnesium sulfate heptahydrate, 2g of manganese chloride tetrahydrate, 1g of potassium phosphate, 1g of dipotassium hydrogen phosphate, 0.7g of disodium magnesium ethylenediamine tetraacetate, 0.5g of sodium permanganate and 0.3g of zinc sulfate heptahydrate, adding into 500ml of deionized water, stirring and dissolving, continuing to add deionized water until the total amount is 1L, and carrying out high-pressure sterilization at 121 ℃ for 15min to obtain a co-culture medium for later use;
controlling the inoculation ratio of anabaena strain to be 5:100 (g/ml), the inoculation ratio of yeast thallus to be 2:100 (g/ml), the inoculation ratio of lactobacillus thallus to be 2:100 (g/ml), the inoculation ratio of bacillus thallus to be 1:100 (g/ml), firstly inoculating anabaena strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h to 10h (light: dark), the shaking table speed to be 150rpm, inoculating the yeast thallus, the lactobacillus thallus and the bacillus thallus after shake culture for 24h, keeping the culture condition unchanged, and centrifuging for 15min under the condition of 1000rpm after continuing to co-culture for 10d, and collecting solid products and liquid products;
s12, adding deionized water at the temperature of 68 ℃ into corn starch according to the feed-liquid ratio of 1:18, stirring and mixing uniformly to obtain a suspension, keeping the state of heat preservation and stirring, adding liquid product with the mass of 0.7 times of the corn starch, glycerol with the mass of 0.3 times of the corn starch and calcium chloride with the mass of 0.05 times of the corn starch, stirring and mixing uniformly, and adding humic acid solution with the mass concentration of 12% to adjust the pH value to 6.8 to obtain a coating liquid;
S13, adding the solid product into the wrapping liquid according to the mass ratio of 1:4, uniformly stirring and dispersing, controlling the spray drying temperature to be 50 ℃, and performing spray drying to obtain a composite biological preparation with the particle size of 60 meshes;
S2, preparing oyster shell matrixes:
s21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 60-mesh sieve to obtain oyster shell powder;
s22, placing oyster shell powder at a temperature of 500 ℃ under a nitrogen atmosphere, and performing heat activation treatment for 2 hours to obtain an activated product;
S23, adding citric acid solution with the mass concentration of 7% into the activated substance, soaking for 3 hours, filtering, washing to be neutral, and drying to be constant weight at the temperature of 110 ℃ to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
Mixing 30 parts of desulfurized gypsum, 50 parts of coconut chaff and 50 parts of bagasse, grinding, sieving with a 30-mesh sieve, adding 12 parts of a composite biological agent, 25 parts of oyster shell matrix, 3 parts of monoammonium phosphate and 2 parts of potassium humate, uniformly mixing, and packaging to obtain the saline-alkali soil modifier.
In order to verify the comprehensive performance of the saline-alkali soil improver in examples 1 to 5 of the present application, the applicant set comparative examples 1 to 5, specifically as follows:
comparative example 1
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
Adding 20g of glucose, 15g of calcium chloride dihydrate, 9g of sodium carbonate, 2g of ferric ammonium citrate, 2g of sodium alginate, 1g of ammonium sulfate, 1g of magnesium sulfate heptahydrate, 1.5g of manganese chloride tetrahydrate, 0.5g of potassium phosphate, 0.5g of dipotassium hydrogen phosphate, 0.3g of disodium magnesium ethylenediamine tetraacetate, 0.2g of sodium permanganate and 0.1g of zinc sulfate heptahydrate into 500ml of deionized water, stirring and dissolving, continuously adding deionized water until the total amount is 1L, and carrying out high-pressure sterilization at 121 ℃ for 15min to obtain a co-culture medium for later use;
Controlling the inoculation ratio of the anabaena strain, the saccharomycete strain, the lactic acid bacteria strain and the bacillus strain to be 10:100 (g/ml), respectively inoculating the anabaena strain, the saccharomycete strain, the lactic acid bacteria strain and the bacillus strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h:10h (light: dark), the shaking table speed to be 150rpm, keeping the culture condition unchanged after shaking culture for 24h, continuously culturing the anabaena strain for 10d, continuously culturing the saccharomycete strain, the lactic acid bacteria strain and the bacillus strain for 9d, centrifuging for 15min under the condition of 1000rpm after the respective culture is finished, and collecting and obtaining anabaena solid products and anabaena liquid products, saccharomycete solid products and saccharomycete liquid products, lactic acid bacteria solid products and lactic acid bacteria liquid products, bacillus solid products and bacillus liquid products;
S12, blending the anabaena solid product, the saccharomycete solid product, the lactobacillus solid product and the bacillus solid product according to a mass ratio of 5:2:2:1 to obtain a total solid product; blending the anabaena liquid product, the saccharomycete liquid product, the lactobacillus liquid product and the bacillus liquid product according to the mass ratio of 5:2:2:1 to obtain a total liquid product;
S13, adding deionized water with the mass concentration of 8% into corn starch according to the feed-liquid ratio of 1:14, stirring and mixing uniformly to obtain a suspension, keeping the state of heat preservation and stirring, adding 0.5 times of total liquid product with the mass of corn starch, 0.1 time of glycerol with the mass of corn starch and 0.03 time of calcium chloride with the mass of corn starch, stirring and mixing uniformly, and adding a humic acid solution with the mass concentration of 8% to adjust the pH value to 5.7 to obtain a coating liquid;
S14, adding the total solid product into the wrapping liquid according to the mass ratio of 1:3, uniformly stirring and dispersing, controlling the spray drying temperature to be 30 ℃, and performing spray drying to obtain a composite biological preparation with the particle size of 40 meshes;
S2, preparing oyster shell matrixes:
S21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 40-mesh sieve to obtain oyster shell powder;
S22, placing oyster shell powder at 400 ℃ under nitrogen atmosphere, and performing heat activation treatment for 4 hours to obtain an activated product;
S23, adding citric acid solution with the mass concentration of 3% into the activated substance, soaking for 5 hours, filtering, washing to be neutral, and drying to be constant weight at the temperature of 110 ℃ to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
mixing and grinding 20 parts of desulfurized gypsum, 40 parts of coconut chaff and 40 parts of bagasse, sieving with a 10-mesh sieve, adding 8 parts of a composite biological agent, 15 parts of oyster shell matrix, 1 part of monoammonium phosphate and 0.5 part of potassium humate, uniformly mixing, and sub-packaging to obtain the saline-alkali soil modifier.
Comparative example 2
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
Adding 20g of glucose, 15g of calcium chloride dihydrate, 9g of sodium carbonate, 2g of ferric ammonium citrate, 2g of sodium alginate, 1g of ammonium sulfate, 1g of magnesium sulfate heptahydrate, 1.5g of manganese chloride tetrahydrate, 0.5g of potassium phosphate, 0.5g of dipotassium hydrogen phosphate, 0.3g of disodium magnesium ethylenediamine tetraacetate, 0.2g of sodium permanganate and 0.1g of zinc sulfate heptahydrate into 500ml of deionized water, stirring and dissolving, continuously adding deionized water until the total amount is 1L, and carrying out high-pressure sterilization at 121 ℃ for 15min to obtain a co-culture medium for later use;
controlling the inoculation ratio of anabaena strain to be 5:100 (g/ml), the inoculation ratio of yeast thallus to be 2:100 (g/ml), the inoculation ratio of lactobacillus thallus to be 2:100 (g/ml), the inoculation ratio of bacillus thallus to be 1:100 (g/ml), firstly inoculating anabaena strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h to 10h (light: dark), the shaking table speed to be 150rpm, inoculating the yeast thallus, the lactobacillus thallus and the bacillus thallus after shake culture for 24h, keeping the culture condition unchanged, and centrifuging for 15min under the condition of 1000rpm after continuing to co-culture for 10d, and collecting solid products and liquid products;
S12, adding deionized water at 58 ℃ into corn starch according to a feed-liquid ratio of 1:14, stirring and mixing uniformly to obtain a suspension, keeping a heat preservation stirring state, adding glycerol with the mass of 0.1 times of that of the corn starch and calcium chloride with the mass of 0.03 times of that of the corn starch, stirring and mixing uniformly, and adding humic acid solution with the mass concentration of 8% to adjust the pH value to 5.7 to obtain a coating liquid;
s13, adding a liquid product with the mass of 0.5 times of that of the corn starch into the wrapping liquid, adding a solid product, stirring and dispersing uniformly, controlling the spray drying temperature to be 30 ℃, and performing spray drying to obtain a composite biological agent with the particle size of 40 meshes, wherein the mass of the solid product is controlled to be 1:3 in the total amount ratio of the liquid product to the wrapping liquid;
S2, preparing oyster shell matrixes:
S21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 40-mesh sieve to obtain oyster shell powder;
S22, placing oyster shell powder at 400 ℃ under nitrogen atmosphere, and performing heat activation treatment for 4 hours to obtain an activated product;
S23, adding citric acid solution with the mass concentration of 3% into the activated substance, soaking for 5 hours, filtering, washing to be neutral, and drying to be constant weight at the temperature of 110 ℃ to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
mixing and grinding 20 parts of desulfurized gypsum, 40 parts of coconut chaff and 40 parts of bagasse, sieving with a 10-mesh sieve, adding 8 parts of a composite biological agent, 15 parts of oyster shell matrix, 1 part of monoammonium phosphate and 0.5 part of potassium humate, uniformly mixing, and sub-packaging to obtain the saline-alkali soil modifier.
Comparative example 3
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
Adding 20g of glucose, 15g of calcium chloride dihydrate, 9g of sodium carbonate, 2g of ferric ammonium citrate, 2g of sodium alginate, 1g of ammonium sulfate, 1g of magnesium sulfate heptahydrate, 1.5g of manganese chloride tetrahydrate, 0.5g of potassium phosphate, 0.5g of dipotassium hydrogen phosphate, 0.3g of disodium magnesium ethylenediamine tetraacetate, 0.2g of sodium permanganate and 0.1g of zinc sulfate heptahydrate into 500ml of deionized water, stirring and dissolving, continuously adding deionized water until the total amount is 1L, and carrying out high-pressure sterilization at 121 ℃ for 15min to obtain a co-culture medium for later use;
controlling the inoculation ratio of anabaena strain to be 5:100 (g/ml), the inoculation ratio of yeast thallus to be 2:100 (g/ml), the inoculation ratio of lactobacillus thallus to be 2:100 (g/ml), the inoculation ratio of bacillus thallus to be 1:100 (g/ml), firstly inoculating anabaena strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h to 10h (light: dark), the shaking table speed to be 150rpm, inoculating the yeast thallus, the lactobacillus thallus and the bacillus thallus after shake culture for 24h, keeping the culture condition unchanged, and centrifuging for 15min under the condition of 1000rpm after continuing to co-culture for 10d, and collecting solid products and liquid products;
S12, adding deionized water at 58 ℃ into corn starch according to a feed-liquid ratio of 1:14, stirring and mixing uniformly to obtain a suspension, keeping a heat preservation stirring state, adding a liquid product with the mass of 0.5 times of the corn starch and glycerol with the mass of 0.13 times of the corn starch, stirring and mixing uniformly, and adding a humic acid solution with the mass concentration of 8% to adjust the pH value to 5.7 to obtain a coating liquid;
S13, adding the solid product into the wrapping liquid according to the mass ratio of 1:3, uniformly stirring and dispersing, controlling the spray drying temperature to be 30 ℃, and performing spray drying to obtain a composite biological preparation with the particle size of 40 meshes;
S2, preparing oyster shell matrixes:
S21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 40-mesh sieve to obtain oyster shell powder;
S22, placing oyster shell powder at 400 ℃ under nitrogen atmosphere, and performing heat activation treatment for 4 hours to obtain an activated product;
S23, adding citric acid solution with the mass concentration of 3% into the activated substance, soaking for 5 hours, filtering, washing to be neutral, and drying to be constant weight at the temperature of 110 ℃ to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
mixing and grinding 20 parts of desulfurized gypsum, 40 parts of coconut chaff and 40 parts of bagasse, sieving with a 10-mesh sieve, adding 8 parts of a composite biological agent, 15 parts of oyster shell matrix, 1 part of monoammonium phosphate and 0.5 part of potassium humate, uniformly mixing, and sub-packaging to obtain the saline-alkali soil modifier.
Comparative example 4
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
Adding 20g of glucose, 15g of calcium chloride dihydrate, 9g of sodium carbonate, 2g of ferric ammonium citrate, 2g of sodium alginate, 1g of ammonium sulfate, 1g of magnesium sulfate heptahydrate, 1.5g of manganese chloride tetrahydrate, 0.5g of potassium phosphate, 0.5g of dipotassium hydrogen phosphate, 0.3g of disodium magnesium ethylenediamine tetraacetate, 0.2g of sodium permanganate and 0.1g of zinc sulfate heptahydrate into 500ml of deionized water, stirring and dissolving, continuously adding deionized water until the total amount is 1L, and carrying out high-pressure sterilization at 121 ℃ for 15min to obtain a co-culture medium for later use;
controlling the inoculation ratio of anabaena strain to be 5:100 (g/ml), the inoculation ratio of yeast thallus to be 2:100 (g/ml), the inoculation ratio of lactobacillus thallus to be 2:100 (g/ml), the inoculation ratio of bacillus thallus to be 1:100 (g/ml), firstly inoculating anabaena strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h to 10h (light: dark), the shaking table speed to be 150rpm, inoculating the yeast thallus, the lactobacillus thallus and the bacillus thallus after shake culture for 24h, keeping the culture condition unchanged, and centrifuging for 15min under the condition of 1000rpm after continuing to co-culture for 10d, and collecting solid products and liquid products;
S12, adding deionized water at 58 ℃ into corn starch according to a feed-liquid ratio of 1:14, stirring and mixing uniformly to obtain a suspension, keeping a heat preservation stirring state, adding liquid product with the mass of 0.5 times of the corn starch, glycerol with the mass of 0.1 time of the corn starch and calcium chloride with the mass of 0.03 time of the corn starch, stirring and mixing uniformly, and adding humic acid solution with the mass concentration of 8% to adjust the pH value to 5.7 to obtain a coating liquid;
S13, adding the solid product into the wrapping liquid according to the mass ratio of 1:3, uniformly stirring and dispersing, controlling the spray drying temperature to be 30 ℃, and performing spray drying to obtain a composite biological preparation with the particle size of 40 meshes;
S2, preparing oyster shell matrixes:
S21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 40-mesh sieve to obtain oyster shell powder;
s22, adding citric acid solution with the mass concentration of 3% into oyster shell powder, soaking for 5 hours, filtering, washing to be neutral, and drying to constant weight at the temperature of 110 ℃ to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
mixing and grinding 20 parts of desulfurized gypsum, 40 parts of coconut chaff and 40 parts of bagasse, sieving with a 10-mesh sieve, adding 8 parts of a composite biological agent, 15 parts of oyster shell matrix, 1 part of monoammonium phosphate and 0.5 part of potassium humate, uniformly mixing, and sub-packaging to obtain the saline-alkali soil modifier.
Comparative example 5
The preparation method of the saline-alkali soil modifier comprises the following preparation steps:
s1, preparing a composite biological preparation:
s11, controlling the initial algae density to be 1.8X10 3 cells/mL, taking nitrogen fixation anabaena FACHB-119, pre-culturing with 100mL of BG11 culture medium HB8793, controlling the culture temperature to be 25 ℃, the illumination intensity to be 2800 lux, the illumination period to be 12h:12h (light: dark), shaking the shaking table at 180rpm for 72h, centrifuging for 5min under the condition of 8000rpm, discarding the supernatant, and collecting anabaena strain;
Pre-culturing Candida olive CICC 31860, lactobacillus plantarum ACCC 11095 and Bacillus megaterium CICC 10055 with 100ml broth culture medium A-HB8409 at 32deg.C and shaking culture rate of 120rpm for 24 hr, centrifuging at 8000rpm for 5min, discarding supernatant, and collecting yeast thallus, lactobacillus thallus and Bacillus megaterium thallus;
Adding 20g of glucose, 15g of calcium chloride dihydrate, 9g of sodium carbonate, 2g of ferric ammonium citrate, 2g of sodium alginate, 1g of ammonium sulfate, 1g of magnesium sulfate heptahydrate, 1.5g of manganese chloride tetrahydrate, 0.5g of potassium phosphate, 0.5g of dipotassium hydrogen phosphate, 0.3g of disodium magnesium ethylenediamine tetraacetate, 0.2g of sodium permanganate and 0.1g of zinc sulfate heptahydrate into 500ml of deionized water, stirring and dissolving, continuously adding deionized water until the total amount is 1L, and carrying out high-pressure sterilization at 121 ℃ for 15min to obtain a co-culture medium for later use;
controlling the inoculation ratio of anabaena strain to be 5:100 (g/ml), the inoculation ratio of yeast thallus to be 2:100 (g/ml), the inoculation ratio of lactobacillus thallus to be 2:100 (g/ml), the inoculation ratio of bacillus thallus to be 1:100 (g/ml), firstly inoculating anabaena strain into a co-culture medium, controlling the culture temperature to be 30 ℃, the illumination intensity to be 2200 lux, the illumination period to be 14h to 10h (light: dark), the shaking table speed to be 150rpm, inoculating the yeast thallus, the lactobacillus thallus and the bacillus thallus after shake culture for 24h, keeping the culture condition unchanged, and centrifuging for 15min under the condition of 1000rpm after continuing to co-culture for 10d, and collecting solid products and liquid products;
S12, adding deionized water at 58 ℃ into corn starch according to a feed-liquid ratio of 1:14, stirring and mixing uniformly to obtain a suspension, keeping a heat preservation stirring state, adding liquid product with the mass of 0.5 times of the corn starch, glycerol with the mass of 0.1 time of the corn starch and calcium chloride with the mass of 0.03 time of the corn starch, stirring and mixing uniformly, and adding humic acid solution with the mass concentration of 8% to adjust the pH value to 5.7 to obtain a coating liquid;
S13, adding the solid product into the wrapping liquid according to the mass ratio of 1:3, uniformly stirring and dispersing, controlling the spray drying temperature to be 30 ℃, and performing spray drying to obtain a composite biological preparation with the particle size of 40 meshes;
S2, preparing oyster shell matrixes:
S21, recovering fresh oyster shells, washing with water at normal temperature, drying at 60 ℃ until the weight is constant, crushing the dried oyster shells, and sieving with a 40-mesh sieve to obtain oyster shell powder;
S22, adding citric acid solution with the mass concentration of 3% into oyster shell powder, soaking for 5 hours, filtering, washing to be neutral, and drying to be constant weight at the temperature of 110 ℃ to obtain pretreated oyster shell powder;
s23, placing the pretreated oyster shell powder at 400 ℃ under the nitrogen atmosphere, and performing heat activation treatment for 4 hours to obtain an oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
mixing and grinding 20 parts of desulfurized gypsum, 40 parts of coconut chaff and 40 parts of bagasse, sieving with a 10-mesh sieve, adding 8 parts of a composite biological agent, 15 parts of oyster shell matrix, 1 part of monoammonium phosphate and 0.5 part of potassium humate, uniformly mixing, and sub-packaging to obtain the saline-alkali soil modifier.
Saline-alkali soil improvement test
In order to verify the saline-alkali soil improving effect of the saline-alkali soil improving agent in the embodiments 1-5 and the comparative examples 1-5, scientific basis is provided for popularization and application of the saline-alkali soil improving agent by carrying out field test demonstration.
1. Overview of the test site
The experimental land is located on a Daralte Wang Aizhao village saline-alkali soil comprehensive utilization technological innovation experimental demonstration base of Zhenxi village, and is yellow and south shore, and the area belongs to continental arid and semiarid climates, the annual average temperature is 7 ℃, the total annual precipitation amount is 350mm, and the experimental land is mainly concentrated in 7-9 months, the frost-free period is 140d, the altitude 1132m, the groundwater level is 2-3m and the annual sunshine hours are more than 2806 hours. The soil salinity type is mainly soda salinized soil and sulfate-chloride salinized soil, and the pH and the alkalization degree are generally higher. The test soil is clay loam, belongs to soda chloride sulfate saline soil, and has the basic properties shown in the following tables 1 and 2:
TABLE 1 soil salinity characterization
| Hierarchy of layers | CO3 2-（g/kg） | HCO3 -（g/kg） | Ca2+（g/kg） | Mg2+（g/kg） | Cl-（g/kg） | SO4 2-（g/kg） | K++ Na+（g/kg） | Full salt (g/kg) |
| 0-20cm | 0.00 | 0.58 | 0.12 | 0.15 | 1.09 | 2.49 | 1.69 | 6.12 |
| 20-40cm | 0.00 | 1.05 | 0.20 | 0.13 | 1.36 | 2.75 | 1.99 | 7.48 |
TABLE 2 basic physicochemical Properties of soil
| Hierarchy of layers | Bulk density (g/cm 3) | Cation exchange capacity (cmol/kg) | pH | Organic matter (g/kg) | Total nitrogen (g/kg) | Alkaline hydrolysis nitrogen (mg/kg) | Quick-acting phosphorus (mg/kg) | Quick-acting potassium (mg/kg) |
| 0-20cm | 1.43 | 12.68 | 8.95 | 12.09 | 0.58 | 31.60 | 12.22 | 174.00 |
| 20-40cm | 1.55 | 9.61 | 9.12 | 8.28 | 0.37 | 18.24 | 2.90 | 133.67 |
2. Test design
2.1 The application is provided with 11 test lands, the area of each test land is 10 mu, the test lands are respectively marked as test lands 1-5, comparison test lands 1-5 and comparison test lands 1-5, the saline-alkali soil modifier prepared by the embodiment 1-5 and the comparison example 1-5 is used for fertilization, and the comparison test lands are respectively fertilized by adopting nitrogen-phosphorus-potassium organic fertilizers (N: P: K=15: 15, total nutrients are 45%).
2.2 The tested crops are sunflower (variety: same day No. 5), the large row spacing is 100cm, the small row spacing is 40cm, the plant spacing is 60cm, seedlings 1588 plants are reserved in acre, the irrigation time is 0, the times are 0, and the irrigation quantity is 0m 3/acre.
The fertilizer for the test lands is applied once along with the furrow irrigation at 30 kg/mu in 20 days of 5 months, and unified field management measures are carried out on each test land; sunflower was grown on day 6, 15, and harvested on day 10, 12; and collecting soil samples of each test site in layers of 0-20cm and 20-40cm during harvesting.
3. Test results and analysis
3.1 Detailed observations were made during each growth period of sunflower, and the observations and recordings were shown in table 3 below:
TABLE 3 Table for observing and recording the growth period of sunflower in different test sites
As can be seen from the data shown in table 3: the difference of the observation and record data of the sunflowers in the seedling stage of each test site is not obvious, and the main reason is that the sunflower in the seedling stage has small fertilizer requirement, and the nutrients are mainly provided by soil foundation soil fertility; whereas sunflower plant height in trials 1-5 was significantly higher than in the control trials 1-5 and the control trials during flowering and maturity.
3.2 Sampling and yield measurement were performed at day 9 and 30, and yield-related data are shown in table 4 below:
TABLE 4 sunflower yield recordings from different trials
| Test floor | Hundred weight/g | Mu yield/kg/mu |
| Test floor 1 | 14.38 | 175.62 |
| Test floor 2 | 14.43 | 176.09 |
| Test floor 3 | 15.61 | 182.04 |
| Test floor 4 | 14.90 | 178.62 |
| Test floor 5 | 14.77 | 177.78 |
| Comparative test floor 1 | 9.73 | 130.03 |
| Comparative test floor 2 | 10.45 | 138.39 |
| Comparative test floor 3 | 11.52 | 150.81 |
| Comparative test floor 4 | 12.26 | 157.28 |
| Comparative test floor 5 | 12.61 | 160.06 |
| Control test land | 11.62 | 152.69 |
As can be seen from the data shown in table 4: the saline-alkali soil modifier obtained in examples 1-5 of the present application has far better effect on sunflower yield and quality than comparative examples 1-5. Compared with the control test, the yield of the test land is improved by 15.01 percent, the hundred-grain weight is improved by 23.75 percent, and the yield and the quality of the sunflower are obviously improved.
3.3 Soil samples of each test site were collected and the soil nutrient parameters were recorded as follows in table 5:
Table 5 soil nutrient Change Table for each test site
As can be seen from the data shown in table 5: the saline-alkali soil modifier obtained in the embodiments 1-5 of the application can obviously improve soil nutrients, reduce total salt content, and greatly improve saline-alkali soil restoration effect compared with the saline-alkali soil modifier obtained in the comparative embodiments 1-5.
The present embodiment is only for explanation of the present application and is not to be construed as limiting the present application, and modifications to the present embodiment, which may not creatively contribute to the present application as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present application.
Claims (7)
1. The preparation method of the saline-alkali soil modifier is characterized by comprising the following preparation steps:
s1, preparing a composite biological preparation:
S11, after the anabaena and the mixed bacteria are respectively pre-cultured, co-culturing in a co-culture medium, and after the culture period is finished, centrifugally collecting a solid product and a liquid product;
S12, adding deionized water into corn starch, stirring and mixing to obtain a suspension, adding a liquid product, glycerol and calcium chloride into the suspension, stirring and mixing, and adding acid liquor I to adjust the pH value to 5.7-6.8 to obtain a coating liquid;
S13, adding the solid product into the wrapping liquid, stirring and dispersing uniformly, and then spray-drying to obtain a composite biological preparation;
s2, preparing oyster shell matrix
S21, recovering fresh oyster shells, washing with water, drying, crushing and sieving to obtain oyster shell powder;
S22, performing heat activation treatment on oyster shell powder to obtain an activator;
s23, soaking the activated substance in acid liquor II, washing with water to be neutral, and drying to constant weight to obtain oyster shell matrix;
s3, preparing a saline-alkali soil modifier:
Mixing, grinding and sieving the desulfurized gypsum, the coconut chaff and the bagasse, and adding the composite biological agent, the oyster shell matrix, the monoammonium phosphate and the potassium humate to be uniformly mixed to obtain the saline-alkali soil modifier;
the anabaena in the step S11 is nitrogen fixation anabaena; the mixed bacteria are candida olive, lactobacillus plantarum and bacillus megaterium.
2. The method for preparing a saline-alkali soil improver according to claim 1, wherein the co-culture medium in the step S11 comprises the following components: according to 1L, 20-24g of glucose, 15-18g of calcium chloride dihydrate, 9-11g of sodium carbonate, 2-5g of ferric ammonium citrate, 2-4g of sodium alginate, 1-3g of ammonium sulfate, 1-2g of magnesium sulfate heptahydrate, 1.5-2g of manganese chloride tetrahydrate, 0.5-1g of potassium phosphate, 0.5-1g of dipotassium hydrogen phosphate, 0.3-0.7g of disodium magnesium ethylenediamine tetraacetate, 0.2-0.5g of sodium permanganate, 0.1-0.3g of zinc sulfate heptahydrate and the balance of deionized water.
3. The method for preparing a saline-alkali soil improver according to claim 1, wherein the co-culture conditions in the step S11 are as follows: the culture temperature is 25-33 ℃, the illumination intensity is 2000-3000 lux, the illumination period is 14h light/10 h dark, the shaking table speed is 120-160rpm, and the culture time is 24h.
4. The method for preparing a saline-alkali soil conditioner according to claim 1, wherein the acid solution I in the step S12 is humic acid solution with a mass concentration of 8-12%.
5. The method for preparing a saline-alkali soil conditioner according to claim 1, wherein the acid solution II in the step S23 is a citric acid solution with a mass concentration of 3-7%.
6. The method for preparing a saline-alkali soil improver according to claim 1, wherein the raw materials used in the step S3 are as follows: the composite biological agent comprises, by weight, 20-30 parts of desulfurized gypsum, 40-50 parts of coconut husk, 40-50 parts of bagasse, 8-12 parts of a composite biological agent, 15-25 parts of oyster shell matrix, 1-3 parts of monoammonium phosphate and 0.5-2 parts of potassium humate.
7. A saline-alkali soil conditioner characterized by being prepared by the method for preparing the saline-alkali soil conditioner according to any one of claims 1 to 6.
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