CN116297427A - Exosome concentration detection kit - Google Patents
Exosome concentration detection kit Download PDFInfo
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- CN116297427A CN116297427A CN202310224846.2A CN202310224846A CN116297427A CN 116297427 A CN116297427 A CN 116297427A CN 202310224846 A CN202310224846 A CN 202310224846A CN 116297427 A CN116297427 A CN 116297427A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides an exosome concentration detection kit, which comprises a detection kit body, wherein the detection kit body comprises a kit body, a cover plate, a detection platform, a reagent storage mechanism and a sample injection mechanism, the detection platform is arranged in the kit body, the reagent storage mechanism is arranged on one side of the detection platform, a reagent storage bin is arranged in the reagent storage mechanism, the sample injection mechanism is arranged on the other side of the detection platform, the sample storage bin is arranged in the sample injection mechanism, the exosome concentration detection kit is used for arranging the detection platform in the sample injection mechanism, when the detection is used, the mixing process can be completed by directly synchronously injecting working solution on the side and exosome solution samples, the detection flow is simplified, the detection efficiency is improved, independent test tubes in each row can be sequentially injected through a puncture assembly, and the two solution amounts in each independent test tube can reach preset standards.
Description
Technical Field
The invention relates to the technical field of exosome detection, in particular to an exosome concentration detection kit.
Background
Exosomes refer to small vesicles comprising complex RNAs and proteins, particularly disc vesicles with diameters of 40-100 nm. Exosomes are considered as specifically secreted vesicles that are involved in intercellular communication, and research into exosomes helps to understand how they are used in the development of minimally invasive diagnostics.
The detection process of the exosome concentration is based on the principle of measuring the protein concentration by bca method: after working solution is mixed with an exosome solution sample in a contact way, cu < 2+ > is reduced into Cu < + >, BCA chelated Cu < + > is used as a color developing agent by using a biuret principle under alkaline conditions, bluish violet color is generated, absorption peaks exist at 562nm, and monovalent Cu < + > and the protein are in dose correlation.
After absorbance is measured to the mixed solution, substituting into standard curve, can calculate sample concentration, this in-process needs to use corresponding exosome concentration detection kit, among the prior art, directly store the inside at the kit with the working solution, take out and use on independent testing platform during the use, this process is wasted time and energy, the kit also only can play the effect of packing and accomodating, the functional singleness, can't assist the mixed process in the follow-up measurement in-process, on the other hand, after the survey is accomplished, need with the help of outside storage device to testing platform's sealed preservation process, the test cost has been increased.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the exosome concentration detection kit for solving the problems in the background art, is convenient to use, can realize a high-efficiency and accurate test process, and effectively reduces the waste of reagents or samples.
In order to achieve the above object, the present invention is realized by the following technical scheme: the utility model provides an exosome concentration detection kit, includes the detection kit body, the detection kit body includes box body, apron, testing platform, reagent storage mechanism and sample injection mechanism, the internally mounted of box body has testing platform, reagent storage mechanism is installed to one side of testing platform, reagent storage mechanism's inside is provided with reagent storage storehouse, sample injection mechanism is installed to testing platform's opposite side, sample injection mechanism's inside is provided with sample storage storehouse, a plurality of independent test tubes are installed to testing platform's bottom, the side top of box body is through pivot and apron part swing joint, the shutoff subassembly is installed to the inboard of apron, guide sleeve is all installed at reagent storage mechanism and sample injection mechanism's top, every guide sleeve's inside is all installed puncture subassembly, reagent storage storehouse or sample storage storehouse's inside is inserted to puncture subassembly's bottom.
Further, the testing platform comprises an independent test tube and a pore plate, wherein the independent test tube is arranged at the bottom of the pore plate, a tube orifice is arranged at the top of the independent test tube, an injection opening is formed in the inner wall of the independent test tube, and two ends of the pore plate are respectively fixed with the reagent storage mechanism and the sample injection mechanism to form a whole.
Further, independent test tubes are provided with a plurality of, and the interval between every adjacent independent test tubes is the same, the top of orificial top flushes with the top of orifice plate, the side of orifice plate is laminated with the inner wall of box body mutually.
Further, the shutoff subassembly includes seal post and rubber conical surface, the rubber conical surface sets up at the top of seal post, the seal post inserts inside every independent test tube that corresponds through the upset of apron, and the filling port covers through the surface of seal post and shelters from.
Further, reagent storage mechanism includes reagent storage storehouse and first baffle, first baffle fixed mounting is in the inside of reagent storage storehouse, and is provided with independent storage cavity between two adjacent first baffles, the side of reagent storage storehouse is connected with reagent pipeline.
Further, the reagent conveying pipeline penetrates out of the side edge of the reagent storage bin, and the reagent conveying pipeline is communicated with the plurality of injection opening parts along the inner side of the pore plate.
Further, sample injection mechanism includes sample storage storehouse and second baffle, the inside at sample storage storehouse is installed to the second baffle, and is provided with independent storage cavity between two adjacent second baffles, every puncture subassembly is all installed at the top of independent storage cavity, and sealing membrane has all been laid to the bottom of every independent storage cavity.
Further, the side of first baffle is all fixed as wholly with the inner wall of reagent storage storehouse, and the inside of reagent storage storehouse is filled with the working solution, be provided with the clearance between the inner wall of second baffle's top and sample storage storehouse, sample injection hole is installed at the one end top of sample storage storehouse, anti-overflow appearance chamber is installed to the other end of sample storage storehouse, anti-overflow appearance chamber's top is linked together with the inside of sample storage storehouse through anti-overflow pipeline.
Further, the puncture assembly comprises a push rod and a puncture cone block, wherein the push block is arranged at the top of the push rod, the push rod penetrates through the guide sleeve, and the puncture cone block is arranged at the bottom end of the push rod.
Further, a limiting baffle is arranged at the joint of the puncture cone block and the bottom of the ejector rod, and the bottom of each puncture assembly is aligned with the middle position of the sealing film.
The invention has the beneficial effects that: the invention discloses an exosome concentration detection kit, which comprises a detection kit body, wherein the detection kit body comprises a box body, a cover plate, a plugging assembly, a detection platform, a reagent storage mechanism, a sample injection mechanism, an orifice plate, an independent test tube, a tube orifice, a reagent storage bin, a sample storage bin, a puncture assembly, a sample injection hole, an anti-overflow cavity, an independent storage cavity, a first partition plate, an indication mark, a reagent conveying pipeline, a sealing film, a push rod, a push block, a guide sleeve, a limit baffle, a puncture cone block, a second partition plate, a guide plate, a sample conveying pipeline, an anti-overflow pipeline, a rubber conical surface, a sealing column and an injection hole.
1. This exosome concentration detection kit sets up testing platform inside, when detecting and using, directly pours into testing platform's inside with the inside working solution of the reagent storage mechanism of side into to with the synchronous injection of exosome solution sample can accomplish mixing process, detection flow has simplified, has improved the efficiency of detection, and has reduced the degree of difficulty of experimental operation, is convenient for popularize and use.
2. This exosome concentration detection kit can pour into the solution of reagent storage mechanism and sample injection mechanism inside into testing platform through control puncture subassembly in, can pour into the independent test tube of every row in proper order through this structure to ensure that the two kinds of solution volume of the inside final injection of every independent test tube reach preset standard all the time, thereby improved the accuracy of final detection, and reduce the extracorporal solution sample or the extracorporal working solution's of wastage condition.
3. This exosome concentration detection kit installs a plurality of seal posts in the apron inboard, and the testing platform of cooperation bottom can be after accomplishing the detection, closes through closing the apron, can carry out the shutoff with the mouth of pipe at every independent test tube top on the testing platform, and then in subsequent removal in-process, can avoid inside mixed solution of every independent test tube to spill and leak, has improved the leakproofness, and the operation is high-efficient convenient.
Drawings
FIG. 1 is a schematic diagram showing the configuration of an exosome concentration detection kit according to the present invention;
FIG. 2 is a schematic structural diagram of a detection platform part of an exosome concentration detection kit according to the present invention;
FIG. 3 is a cross-sectional view of a portion of a reagent storage mechanism of an exosome concentration detection kit of the present invention;
FIG. 4 is a side cross-sectional view of a portion of a sample injection mechanism of an exosome concentration detection kit according to the present invention;
FIG. 5 is a partial enlarged view of a detection platform of an exosome concentration detection kit according to the present invention;
in the figure: 1. a case body; 2. a cover plate; 3. a plugging assembly; 4. a detection platform; 5. a reagent storage mechanism; 6. a sample injection mechanism; 7. an orifice plate; 8. an independent test tube; 9. a pipe orifice; 10. a reagent storage bin; 11. a sample storage bin; 12. a puncture assembly; 13. a sample injection hole; 14. an anti-overflow cavity; 15. an independent storage cavity; 16. a first separator; 17. an indication mark; 18. a reagent delivery conduit; 19. a sealing film; 20. a push rod; 21. a pushing block; 22. a guide sleeve; 23. a limit baffle; 24. puncturing the cone block; 25. a second separator; 26. a deflector; 27. a sample delivery conduit; 28. an overflow prevention pipe; 29. a rubber conical surface; 30. a sealing column; 31. an injection port.
Detailed Description
The invention is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
Referring to fig. 1 to 5, the present invention provides a technical solution: the utility model provides an exosome concentration detection kit, includes the detection kit body, the detection kit body includes box body 1, apron 2, testing platform 4, reagent storage mechanism 5 and sample injection mechanism 6, the internally mounted of box body 1 has testing platform 4, reagent storage mechanism 5 is installed to one side of testing platform 4, reagent storage mechanism 5's inside is provided with reagent storage storehouse 10, sample injection mechanism 6 is installed to the opposite side of testing platform 4, sample injection mechanism 6's inside is provided with sample storage storehouse 11, a plurality of independent test tubes 8 are installed to testing platform 4's bottom, the side top of box body 1 is through pivot and apron 2 part swing joint, shutoff subassembly 3 is installed to the inboard of apron 2, guide sleeve 22 is all installed at reagent storage mechanism 5 and sample injection mechanism 6's top, every guide sleeve 22's inside is all installed puncture subassembly 12, puncture subassembly 12's bottom inserts reagent storage storehouse 10 or sample storage storehouse 11's inside. The exosome concentration detection kit mainly detects the principle of protein concentration by bca method: in alkaline environments, proteins complex with cu2+ and reduce cu2+ to cu1+. BCA and Cu < 1+ > are combined to form a stable purple blue compound, a high light absorption value is obtained at 562nm and is in direct proportion to the protein concentration, and the protein concentration can be measured, wherein BCA working solution is selected as the working solution, a large amount of Cu < 2+ > and BCA ingredients are contained in the working solution, and an effective alkaline environment is provided, so that a purple blue color can be formed after a working solution reagent is mixed with a sample, and the concentration of an exosome can be represented by judging the depth of the color.
Specifically, the exosome concentration detection kit integrates the detection platform 4, the reagent storage mechanism 5 and the sample injection mechanism 6 in the interior, the working solution required for detection is directly injected into the reagent storage bin 10 during production, and in the subsequent detection experiment, the sealing film 19 at the bottom of the reagent storage mechanism 5 is pierced by manually triggering the piercing assembly 12 at the top, so that the internal working solution can be automatically moved to the detection platform 4 along the reagent conveying pipeline 18 at the bottom, each independent test tube 8 in the detection platform 4 is filled, then the exosome solution sample to be detected in the exterior is injected into the sample injection mechanism 6, the quantitative process is realized in the process, finally, the exosome solution sample after the quantitative process can be conveyed into the interior of each independent test tube 8 along the sample conveying pipeline 27 through the piercing assembly 12 on the sample injection mechanism 6, and the mixing process between the sample and the working solution can be completed, and the final detection aim is realized.
After the mixing process is finished, standing is carried out for 30 minutes, at this time, the solution in each independent test tube 8 forms a purple blue state, and the external colorimetric plate is colorized under the state, so that the concentration condition of an exosome can be preliminarily judged, the mixed solution can be put into an ELISA analyzer, colorimetry is carried out under the wavelength of 562nm, and the concentration of the exosome can be displayed through the output light absorption value.
In this embodiment, the detection platform 4 includes an independent test tube 8 and an orifice 7, the independent test tube 8 is installed at the bottom of the orifice 7, the top of the independent test tube 8 is provided with a tube orifice 9, an injection port 31 is provided on the inner wall of the independent test tube 8, two ends of the orifice 7 are respectively fixed with the reagent storage mechanism 5 and the sample injection mechanism 6 into a whole, the independent test tube 8 is provided with a plurality of adjacent independent test tubes 8, the interval between every two adjacent independent test tubes 8 is the same, the top of the tube orifice 9 is flush with the top of the orifice 7, the side edge of the orifice 7 is attached to the inner wall of the box body 1, the plugging assembly 3 includes a sealing column 30 and a rubber conical surface 29, the rubber conical surface 29 is arranged at the top of the sealing column 30, the sealing column 30 is inserted into the corresponding independent test tube 8 through the overturn of the cover plate 2, the injection port 31 is covered and shielded by the surface of the sealing column 30, a plurality of sealing columns 30 are arranged on the inner side of the cover plate 2 and matched with the detection platform 4 at the bottom, after detection is completed, the pipe orifice 9 at the top of each independent test tube 8 on the detection platform 4 can be plugged by closing the cover plate 2, so that in the subsequent moving process, the mixed solution inside each independent test tube 8 can be prevented from leaking, the tightness is improved, the operation is efficient and convenient, and in particular, the working solution reagent and the exosome solution sample are respectively injected into the inside of each independent test tube 8 on the pore plate 7 through the reagent storage mechanism 5 and the sample injection mechanism 6, the mixing can be completed, the cover plate 2 is closed in the moving process of the whole detection platform 4, the top of each independent test tube 8 can be plugged by utilizing each sealing column 30, avoiding the situation of scattering and leakage in the transferring process.
In this embodiment, the reagent storage mechanism 5 includes a reagent storage bin 10 and a first partition 16, the first partition 16 is fixedly installed inside the reagent storage bin 10, and an independent storage cavity 15 is provided between two adjacent first partitions 16, a reagent delivery pipe 18 is connected to a side edge of the reagent storage bin 10, the reagent delivery pipe 18 penetrates out of the side edge of the reagent storage bin 10, and the reagent delivery pipe 18 is respectively communicated with a plurality of injection ports 31 along an inner side of the orifice 7, specifically, when the injection of the working fluid reagent is performed, the push block 21 is directly pressed to move the puncture assembly 12 downward, and finally, a sealing film 19 at the bottom of the reagent storage bin 10 is punctured, so that the working fluid reagent enters into the inside of the reagent delivery pipe 18 along the guide plate 26, and finally enters into the inside of each independent test tube 8.
In this embodiment, sample injection mechanism 6 includes sample storage storehouse 11 and second baffle 25, second baffle 25 installs in the inside of sample storage storehouse 11, and is provided with independent storage cavity 15 between two adjacent second baffles 25, every puncture subassembly 12 is all installed at the top of independent storage cavity 15, and the sealing membrane 19 has all been laid to the bottom of every independent storage cavity 15, the side of first baffle 16 is all fixed for whole with the inner wall of reagent storage storehouse 10, and the inside of reagent storage storehouse 10 is filled with the working solution, be provided with the clearance between the inner wall of sample storage storehouse 11 at the top of second baffle 25, sample injection hole 13 is installed at the one end top of sample storage storehouse 11, spill proof chamber 14 is installed to the other end of sample storage storehouse 11, spill proof chamber 14's top is linked together with the inside of sample storage storehouse 11 through spill proof pipeline 28, sets up inside with testing platform 4, and when detecting the use, directly injects the inside working solution of reagent storage mechanism 5 of side into testing platform to the inside of testing platform to the volume, and will be mixed with the inner wall of reagent storage storehouse 10 and the volume, and the sample injection hole 13 has been realized to the sample storage cavity is realized to the sample storage storehouse 11 after the sample storage storehouse is moved to the sample storage storehouse 11, and the sample injection hole is moved to the sample injection hole is realized to the sample storage cavity is moved to the sample storage storehouse 11, and the sample injection hole is moved to the sample storage cavity is moved to the sample storage chamber is of sample storage storehouse 11 to the sample storage chamber is of the sample storage storehouse is of the sample volume 13.
In this embodiment, the puncture assembly 12 includes a push rod 20 and a puncture cone block 24, the push rod 21 is installed at the top of the push rod 20, the push rod 20 passes through the guide sleeve 22, the puncture cone block 24 is installed at the bottom end of the push rod 20, a limit baffle 23 is disposed at the connection position between the puncture cone block 24 and the bottom of the push rod 20, the bottom of each puncture assembly 12 is aligned with the middle position of the sealing membrane 19, the solutions inside the reagent storage mechanism 5 and the sample injection mechanism 6 can be injected into the detection platform 4 by controlling the puncture assemblies 12, the independent test tubes 8 of each column can be sequentially injected through the structure, and the two types of solutions finally injected inside each independent test tube 8 always reach the preset standard, so that the accuracy of final detection is improved, the waste condition of external secretion solution samples or working solutions is reduced, the puncture assembly 12 is clamped and kept fixed through the inner wall of the guide sleeve 22 when not in use, the downward pressure is directly applied, the sealing membrane 19 is punctured by the puncture cone block 24 at the bottom, and then the solution can be pulled upwards again, and the solution inside the independent storage cavity 15 can be discharged upwards.
While the fundamental and principal features of the invention and advantages of the invention have been shown and described, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (10)
1. The utility model provides an exosome concentration detection kit, includes detection kit body, its characterized in that: the utility model provides a detection kit body includes box body (1), apron (2), testing platform (4), reagent storage mechanism (5) and sample injection mechanism (6), internally mounted of box body (1) has testing platform (4), reagent storage mechanism (5) are installed to one side of testing platform (4), the inside of reagent storage mechanism (5) is provided with reagent storage storehouse (10), sample injection mechanism (6) are installed to the opposite side of testing platform (4), the inside of sample injection mechanism (6) is provided with sample storage storehouse (11), a plurality of independent test tubes (8) are installed to the bottom of testing platform (4), the side top of box body (1) is through pivot and apron (2) part swing joint, shutoff subassembly (3) are installed to the inboard of apron (2), guide sleeve (22) are all installed at the top of reagent storage mechanism (5) and sample injection mechanism (6), every guide sleeve (22) inside all installs puncture subassembly (12), puncture subassembly (12) bottom insert to detect reagent storage storehouse (10) or sample concentration detection method to the inside of body (48) is carried out to the concentration of protein (bca).
2. The exosome concentration detection kit according to claim 1, wherein: the detection platform (4) comprises an independent test tube (8) and a pore plate (7), wherein the independent test tube (8) is arranged at the bottom of the pore plate (7), a pipe orifice (9) is arranged at the top of the independent test tube (8), an injection opening (31) is formed in the inner wall of the independent test tube (8), and two ends of the pore plate (7) are respectively fixed with the reagent storage mechanism (5) and the sample injection mechanism (6) to form a whole.
3. An exosome concentration detection kit according to claim 2, wherein: independent test tube (8) are provided with a plurality of, and the interval between every adjacent independent test tube (8) is the same, the top of mouth of pipe (9) flushes with the top of orifice plate (7), the side of orifice plate (7) is laminated with the inner wall of box body (1) mutually.
4. A kit for detecting the concentration of exosomes according to claim 3, characterized in that: the plugging assembly (3) comprises a sealing column (30) and a rubber conical surface (29), the rubber conical surface (29) is arranged at the top of the sealing column (30), the sealing column (30) is inserted into each corresponding independent test tube (8) through the overturning of the cover plate (2), and the injection port (31) is covered and shielded through the surface of the sealing column (30).
5. An exosome concentration detection kit according to claim 2, wherein: the reagent storage mechanism (5) comprises a reagent storage bin (10) and first partition boards (16), wherein the first partition boards (16) are fixedly installed inside the reagent storage bin (10), an independent storage cavity (15) is formed between two adjacent first partition boards (16), and a reagent conveying pipeline (18) is connected to the side edge of the reagent storage bin (10).
6. The exosome concentration detection kit according to claim 5, wherein: the reagent conveying pipeline (18) penetrates out of the side edge of the reagent storage bin (10), and the reagent conveying pipeline (18) is respectively communicated with a plurality of injection ports (31) along the inner side of the orifice plate (7).
7. The exosome concentration detection kit according to claim 5, wherein: sample injection mechanism (6) are including sample storage storehouse (11) and second baffle (25), the inside in sample storage storehouse (11) is installed to second baffle (25), and is provided with independent storage cavity (15) between two adjacent second baffles (25), every puncture subassembly (12) are all installed at the top of independent storage cavity (15), and sealing membrane (19) have all been laid to the bottom of every independent storage cavity (15), the bottom outside of sample storage storehouse (11) is connected with a plurality of sample conveying pipeline (27), every the outside of independent storage cavity (15) all is carved with instruction marking (17).
8. The exosome concentration detection kit according to claim 7, wherein: the side of first baffle (16) is all fixed as whole with the inner wall of reagent storage storehouse (10), and the inside of reagent storage storehouse (10) is filled with the working solution, be provided with the clearance between the inner wall of top and sample storage storehouse (11) of second baffle (25), sample injection hole (13) are installed at the one end top of sample storage storehouse (11), anti-overflow appearance chamber (14) are installed at the other end of sample storage storehouse (11), the top of anti-overflow appearance chamber (14) is linked together through anti-overflow pipeline (28) with the inside of sample storage storehouse (11), every the bottom of sealing membrane (19) all is provided with guide plate (26).
9. The exosome concentration detection kit according to claim 7, wherein: the puncture assembly (12) comprises a push rod (20) and a puncture cone block (24), wherein a push block (21) is arranged at the top of the push rod (20), the push rod (20) penetrates through the guide sleeve (22), and the puncture cone block (24) is arranged at the bottom end of the push rod (20).
10. The exosome concentration detection kit according to claim 9, wherein: the connection part of the puncture cone block (24) and the bottom of the ejector rod (20) is provided with a limit baffle (23), the bottom of each puncture assembly (12) is aligned with the middle position of the sealing film (19), and the ejector rod (20) is clamped through an anti-slip layer on the inner wall of the guide sleeve (22) and then is kept fixed.
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| CN202310224846.2A CN116297427A (en) | 2023-03-10 | 2023-03-10 | Exosome concentration detection kit |
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