CN213414908U

CN213414908U - Gene detection kit suitable for MassARRAY platform

Info

Publication number: CN213414908U
Application number: CN202021617182.4U
Authority: CN
Inventors: 黄新文; 王文君; 沈亚平; 朱琳; 胡真真; 杨建滨
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2020-08-06
Filing date: 2020-08-06
Publication date: 2021-06-11
Anticipated expiration: 2030-08-06

Abstract

The utility model provides a gene testing kit suitable for MassARRAY platform, including lid and box body, the box body upper strata is the holding tank, and the lower floor is the cooling cavity, and the holding tank separates into sample processing groove and categorised reagent standing groove again, is equipped with the reagent patchhole in the categorised reagent standing groove, is equipped with flexible elasticity button in the reagent patchhole, and the cooling cavity is located the holding tank under. This kit passes through the change of coolant control reagent standing groove and sample processing groove temperature in the use, and reagent standing groove and sample processing groove divide the setting side by side through sealing the baffle simultaneously, improve the convenience of experiment. The utility model relates to a reasonable in design, through the coating of the different colours that sets up on the categorised reagent standing groove inner wall, can obviously distinguish a plurality of independent application of sample in-process used reagent, make things convenient for follow-up experiment operation to through set up flexible elasticity button in reagent patchhole, distinguish the reagent pipe of use and unused, reduce the error rate of application of sample.

Description

Gene detection kit suitable for MassARRAY platform

Technical Field

The utility model belongs to the technical field of it is biological, a gene detection kit suitable for MassARRAY platform is related to.

Background

The MassARRAY time-of-flight mass spectrum biochip platform integrates the high sensitivity of a PCR technology, the high throughput of a chip technology and the high accuracy of a mass spectrum technology, and is widely applied to the fields of genetic analysis, molecular diagnosis, cancer research, pharmacy and the like. The main experimental steps based on the MassARRAY platform comprise: PCR reaction, SAP digestion reaction, UEP extension, resin purification and on-machine detection can support 96-hole and 384-hole chips and carry out high-throughput detection on a plurality of samples. At present, the reagent pipe in the present reagent box based on MassARRAY platform can not guarantee stability, and the reagent box rocks and can make the reagent pipe roll-off, and the function singleness does not set up the cold liquid of livestock and keeps warm to the reagent, when detecting a plurality of samples, can't avoid the condition of makeing mistakes of application of sample in-process, and sample processing process lacks certain convenience simultaneously, consequently, need design the reagent box based on MassARRAY platform and solve above problem.

Disclosure of Invention

Aiming at the defects of the prior art, the utility model solves the problem of providing a cooling device with cooling function, avoid making mistakes a gene testing kit suitable for MassARRAY platform, comprises a box body and a box cover, the lid is connected with one side of box body through rotating the hinge, the box body upper strata is the holding tank, the lower floor is the cooling cavity, the centre of holding tank separates through the closure plate, the right side of closure plate is the sample processing groove, the left side is equipped with a plurality of categorised reagent standing grooves, every categorised reagent standing groove passes through the partition plate and separates, be equipped with a plurality of reagent patchholes in the categorised reagent standing groove respectively, the cooling cavity is filled with the coolant liquid, the cooling cavity is located the holding tank under, the outside all around and the bottom of cooling cavity are equipped with the heat preservation cavity, the longitudinal section of heat preservation cavity is the U-shaped structure, the inside heat preservation that is equipped with of heat preservation cavity. The closed clapboard is fixedly connected with the sample processing groove and the classification reagent holding groove.

Further, a sponge pad is adhered to the inner wall of the box cover.

Furthermore, the reagent insertion holes are respectively provided with a telescopic elastic button.

Further, the inner walls of the plurality of classification reagent placing grooves are provided with coatings with different colors.

Furthermore, the box cover and the box body are both rectangular structures.

Further, the box cover is connected with the box body through a box cover buckle.

The utility model discloses a set up the cooling cavity under the holding tank of box body to pour into the coolant liquid in the cooling cavity, so can control reagent standing groove and sample processing groove as far as possible in the change of use in-process temperature, reagent standing groove and sample processing groove separate the setting side by side through sealing the baffle simultaneously, have improved the convenience of experiment, the utility model discloses a set up the coating of different colours on the inner wall of categorised reagent standing groove, can obviously distinguish the used reagent of a plurality of independent application of sample in-process, convenient follow-up experiment operation. In addition, the telescopic elastic button is arranged in the reagent inserting hole, the bottom of the reagent tube is in contact with the telescopic elastic button in actual operation, the bottom of the reagent tube is abutted to the telescopic elastic button by pressing the reagent tube, the reagent tube is lifted under the action of the spring, the reagent tube which is pressed and not pressed is different in height, the used and unused reagent tubes are distinguished, and the error rate of sample adding is reduced.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a cross-sectional view of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples.

Example 1

Referring to fig. 1-2, a gene detection kit suitable for a MassARRAY platform, comprising a box body 1 and a box cover 2, wherein the box cover 2 is connected with one side of the box body 1 through a rotating hinge 21, the upper part of the box body 1 is provided with an accommodating groove 17, the lower part of the box body is provided with a cooling cavity 14, the middle of the accommodating groove 17 is separated by a closed partition plate 4, the right side of the closed partition plate 4 is provided with a sample processing groove 11, the left side of the closed partition plate 4 is provided with a plurality of classification reagent placing grooves 12, each classification reagent placing groove 12 is separated by a partition plate 6, a plurality of reagent inserting holes 13 are respectively arranged in the classification reagent placing grooves 12, a cooling liquid is filled in the cooling cavity 14, the cooling cavity 14 is positioned under the accommodating groove 17, the heat preservation cavity 15 is arranged on the outer side and the bottom of the periphery of the cooling cavity 14, the longitudinal section of the heat preservation.

The two ends of the closed partition plate 4 are respectively fixedly connected with the accommodating groove 17 and the classification reagent placing groove 12 in a closed manner; the lower end of the closed partition plate 4 is connected with the bottom of the accommodating groove 17 in a closed mode.

The inner wall of the box cover 2 is stuck with a spongy cushion 3.

Further preferably, the reagent insertion holes 13 are respectively provided with a telescopic elastic button 5 therein, and the telescopic elastic button 5 can be referred to a structure of the prior art, such as a pressing structure of a pen core, and the like, and the test tube can be lifted by pressing the telescopic elastic button 5.

Further preferably, the inner walls of the plurality of sorting reagent placing grooves 12 are provided with coatings of different colors, so as to distinguish different types of reagents.

Further, both the box cover 2 and the box body 1 are rectangular structures. The front side of the box cover 2 is connected with the box body buckle 18 on the front side of the box body 1 through a box cover buckle 22.

The utility model discloses a set up cooling cavity 14 under the holding tank of box body 1, and pour into the cooling cavity 14 into coolant liquid, so can control categorised reagent standing groove 12 and sample processing groove 11 as far as possible in the change of use in temperature, categorised reagent standing groove 12 and sample processing groove 11 carry out the parallel partition setting through sealed baffle 4 simultaneously, have improved the convenience of experiment, the utility model discloses through set up the coating of different colours on the inner wall of categorised reagent standing groove 12, can obviously distinguish the reagent that a plurality of independent application of sample processes used, make things convenient for follow-up experiment operation, in addition through set up flexible elasticity button 5 in reagent patchhole 13, during actual operation, through pressing reagent pipe, make reagent pipe bottom support and press flexible elasticity button 5, reagent pipe rises because of the effect of the spring, thereby appear reagent pipe of different height, and reagent tubes and reagents which are used and unused are distinguished, so that the error rate of sample adding is reduced.

Example 2

The utility model discloses an use, the user at first opens lid 2, select a certain classification reagent standing groove 12, press the reagent pipe of placing in the reagent patchhole 13 wherein lightly, make reagent socle portion touch flexible elasticity button 5, these reagent pipes pop out the take the altitude because of the effect of spring, then get this reagent pipe that risees in batches to sample processing groove 11 and operate, place this reagent pipe of application of sample in original reagent patchhole 13 again after the completion, this reagent pipe in batches is higher than other unprocessed reagent pipes, thereby the clear differentiation is used and is not used reagent pipe. Follow-up experiment application of sample process is similar, and the reagent pipe that will use like this is higher than unused reagent pipe by a certain height, gives the operator in order to indicate whether the reagent in this reagent pipe has been used, even the operation is interrupted, also can not appear repeat application of sample or leak misoperation such as application of sample to reduce the error rate.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A gene detection kit suitable for a MassARRAY platform is characterized by comprising a box body (1) and a box cover (2), wherein the box cover (2) is connected with one side of the box body (1) through a rotating hinge (21), the upper layer of the box body (1) is provided with a containing groove (17), the lower layer of the box body is provided with a cooling cavity (14), the middle of the containing groove (17) is separated through a closed clapboard (4), the right side of the closed clapboard (4) is provided with a sample processing groove (11), the left side of the closed clapboard is provided with a plurality of classification reagent placing grooves (12), each classification reagent placing groove (12) is separated through a partition board (6), a plurality of reagent inserting holes (13) are respectively arranged in each classification reagent placing groove (12), a telescopic elastic button (5) is arranged in each reagent inserting hole (13), the cooling cavity (14) is positioned under the containing groove (17), the outer side and the bottom of the periphery of the cooling, the longitudinal section of the heat-insulating cavity (15) is of a U-shaped structure, and a heat-insulating layer (16) is arranged inside the heat-insulating cavity (15).

2. The gene detection kit suitable for the MassARRAY platform as claimed in claim 1, wherein a sponge pad is adhered to the inner wall of the box cover (2).

3. The gene detection kit applicable to the MassARRAY platform as claimed in claim 1, wherein the heat insulation layer (16) is made of calcium silicate material.

4. The gene detection kit suitable for the MassARRAY platform as claimed in claim 1, wherein the inner wall of the classification reagent placing groove (12) is provided with coatings of different colors.

5. The gene detection kit suitable for the MassARRAY platform as claimed in claim 1, wherein the box body (1) and the box cover (2) are rectangular.

6. The gene detection kit suitable for the MassARRAY platform, according to claim 1, wherein the box cover (2) is connected with the box body buckle (18) through a box cover buckle (22).

7. The gene detection kit suitable for the MassARRAY platform as claimed in claim 1, wherein the cooling cavity (14) is filled with a cooling liquid.