CN113462538A - Anti-pollution nucleic acid detection device - Google Patents
Anti-pollution nucleic acid detection device Download PDFInfo
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- CN113462538A CN113462538A CN202110732456.7A CN202110732456A CN113462538A CN 113462538 A CN113462538 A CN 113462538A CN 202110732456 A CN202110732456 A CN 202110732456A CN 113462538 A CN113462538 A CN 113462538A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 72
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 72
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 72
- 238000001514 detection method Methods 0.000 title abstract description 39
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 101
- 238000006243 chemical reaction Methods 0.000 claims abstract description 82
- 238000007789 sealing Methods 0.000 claims abstract description 52
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical group [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 6
- 229910052782 aluminium Inorganic materials 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 230000000712 assembly Effects 0.000 claims description 2
- 238000000429 assembly Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 29
- 230000008569 process Effects 0.000 abstract description 18
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 description 17
- 238000003199 nucleic acid amplification method Methods 0.000 description 17
- 239000000243 solution Substances 0.000 description 12
- 239000000047 product Substances 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 239000011324 bead Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 239000002985 plastic film Substances 0.000 description 3
- 229920006255 plastic film Polymers 0.000 description 3
- 229920001296 polysiloxane Polymers 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000004411 aluminium Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 2
- 229940069446 magnesium acetate Drugs 0.000 description 2
- 235000011285 magnesium acetate Nutrition 0.000 description 2
- 239000011654 magnesium acetate Substances 0.000 description 2
- 238000007885 magnetic separation Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
- B01L3/50825—Closing or opening means, corks, bungs
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses an anti-pollution nucleic acid detection device, which comprises a plurality of reagent bins, reaction bins and connecting bins, wherein each reagent bin is provided with a reagent storage cavity and a reagent bin opening communicated with the reagent storage cavity, and a sealing assembly is connected to each reagent bin and used for sealing the reagent bin opening; the reaction bin is provided with a reaction cavity for storing a basic reagent; each install respectively on the reagent storehouse connect the storehouse, connect the storehouse and have the linking channel, the linking channel with the reaction chamber intercommunication, the linking channel still extends to seal assembly, sliding connection has the puncture piece on the connection storehouse, the puncture piece is used for the puncture seal assembly is in order to realize the linking channel with the chamber intercommunication is stored to the reagent. The anti-pollution nucleic acid detection device can reduce environmental pollution, reduce operation process and improve detection efficiency.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to an anti-pollution nucleic acid detection device.
Background
In the field of nucleic acid detection, fluorescence PCR and isothermal PCR are commonly used.
The fluorescent PCR is classified into a hands-free method and a magnetic bead method. The hands-free fetching method mainly comprises the following steps: nucleic acid extraction (opening a reaction tube) → PCR amplification, and adding a buffer, a template, primers, a probe and the like in portions (opening the reaction tube three times) → loading. The magnetic bead method mainly comprises the following steps: adding lysis solution (opening a reaction tube) → adding nucleic acid extracting solution (opening the reaction tube) → adding magnetic beads (opening the reaction tube) → adding isopropanol, centrifuging to remove supernatant (opening the reaction tube) → adding washing solution I, centrifuging to remove supernatant (opening the reaction tube) → adding washing solution II, centrifuging to remove supernatant (opening the reaction tube) → placing on a magnetic frame, airing (opening the reaction tube) → adding eluent, shaking, mixing, incubating (opening the reaction tube) → performing magnetic separation, taking the supernatant, and storing in a new centrifugal tube (opening the reaction tube) → PCR amplification → machine.
Constant temperature PCR is classified into a hands-free method and a magnetic bead method. The hands-free fetching method mainly comprises the following steps: nucleic acid extraction (reaction tube opening) → PCR amplification, base reagent, buffer, template, and magnesium acetate are added in portions (reaction tube opening divided by three) → diluent addition (reaction tube opening) → product detection (diluent and amplification product mixed solution, reaction tube opening) → test strip detection (reaction tube opening). The magnetic bead method mainly comprises the following steps: adding lysis solution (opening a reaction tube) → adding nucleic acid extracting solution (opening the reaction tube) → adding magnetic beads (opening the reaction tube) → adding isopropanol, centrifuging to discard a supernatant (opening the reaction tube) → adding washing solution I, centrifuging to discard a supernatant (opening the reaction tube) → adding washing solution II, centrifuging to discard a supernatant (opening the reaction tube) → placing on a magnetic frame, airing (opening the reaction tube) → adding eluent, shaking, mixing, incubating (opening the reaction tube) → performing magnetic separation, taking the supernatant, and storing in a new centrifugal tube (opening the reaction tube) → PCR amplification → test strip detection (opening the reaction tube).
Therefore, in the traditional nucleic acid detection method, the reaction tube needs to be opened repeatedly to add the reagent in the process of nucleic acid amplification after nucleic acid extraction, the reaction tube is opened repeatedly in the nucleic acid amplification process, the environment is easily polluted, and the reaction tube is polluted by partial components in the environment, so that false positive appears in the subsequent detection and diagnosis. In addition, the traditional nucleic acid detection method has complicated operation process and low detection efficiency.
Disclosure of Invention
Therefore, it is necessary to provide an anti-pollution nucleic acid detecting apparatus for solving the problems that in the conventional nucleic acid detecting method, the reaction tube needs to be repeatedly opened to add reagents such as basic reagent and buffer solution, which easily causes environmental and reaction tube pollution, the operation process is complicated, and the detection efficiency is low.
A contamination-preventive nucleic acid detecting device comprising:
the reagent storage device comprises a plurality of reagent bins, a plurality of sealing assemblies and a plurality of control modules, wherein each reagent bin is provided with a reagent storage cavity and a reagent bin opening communicated with the reagent storage cavity, and each reagent bin is connected with one sealing assembly which is used for sealing the reagent bin opening;
the reaction bin is provided with a reaction cavity for storing a basic reagent; and
connect the storehouse, each install respectively on the reagent storehouse connect the storehouse, connect the storehouse and have the interface channel, the interface channel with the reaction chamber intercommunication, the interface channel still extends to seal assembly, sliding connection has the puncture piece on the connection storehouse, the puncture piece is used for the puncture seal assembly is in order to realize the interface channel with the chamber intercommunication is stored to the reagent.
In one embodiment, the connecting bin is arranged at the opening of the reagent bin and covers the sealing component.
In one embodiment, a plurality of the connecting bins are connected to form an integral structure.
In one embodiment, the connecting bin is provided with a connecting groove, a sealing cover is detachably connected to the connecting bin, the sealing cover is connected to the connecting bin and forms the connecting channel with the connecting groove, and the puncturing piece penetrates through the sealing cover and forms a dynamic seal with the sealing cover.
In one embodiment, the outer surface of the sealing cover is provided with a sunken yielding groove, and the puncture piece is partially positioned in the yielding groove, partially penetrates through the bottom surface of the yielding groove and extends into the connecting channel.
In one embodiment, the part of the puncture piece positioned in the abdicating groove protrudes out of the notch of the abdicating groove.
In one embodiment, the parts of the puncture piece located in the receding groove and the parts of the puncture piece located in the connecting channel are both connected with a limiting piece, and the limiting piece protrudes out of the outer peripheral surface of the puncture piece.
In one embodiment, the end of the piercing member facing into the connecting channel is pointed.
In one embodiment, the sealing component is an aluminum film, a plastic plug or a rubber silicone plug.
In one embodiment, the reagent bins are distributed around the reaction bin, the reaction bin is located at one side of the connection bin, and the reagent bin is located at the other side of the connection bin.
According to the anti-pollution nucleic acid detection device, multiple reagents can be stored in each reagent bin independently, the reagents in each reagent bin cannot influence each other, the reaction tube does not need to be opened in the amplification process after nucleic acid extracting solution is injected, after nucleic acid amplification reaction is completed, the reaction tube is opened again to transfer an amplification product to a test strip from the reaction tube, visual detection is carried out through the transparent window, and results are judged and read. Because the reaction tube does not need to be opened in the amplification process after the nucleic acid extracting solution is injected, the environment cannot be polluted, and the pollution of the amplification product to the environment is avoided. In addition, the operation process is reduced without opening the reaction tube in the amplification process, and the detection efficiency is improved. In addition, the operation process is reduced without opening the reaction tube in the amplification process, and the detection efficiency is improved. Above-mentioned anti-pollution nucleic acid detection device is when using, after adding the nucleic acid extract product, the action of sequent addition reagent to need press the puncture piece can, puncture piece puncture behind the seal assembly that corresponds for the reagent chamber in the reagent storehouse that corresponds communicates with being connected, the reagent that corresponds can get into the reaction intracavity through interface channel, need not to open the reaction storehouse, whole in-process only need press the puncture piece can.
Above-mentioned anti-pollution nucleic acid detection device will be a plurality of connect the storehouse and connect and be the integral type structure for the kit structure is compacter.
Above-mentioned anti-pollution nucleic acid detection device has set up the sealed lid of detachable connection, convenient to detach.
Above-mentioned anti-pollution nucleic acid detection device is provided with sunken groove of stepping down on the closing cap to with the puncture piece part be located the inslot of stepping down, puncture piece part is accomodate in the inslot of stepping down and has been reduced the puncture piece and has been exposed in the region of outside, and the groove of stepping down also can effectively protect the puncture piece. In addition, the sealing cover is provided with the abdicating groove, so that the material cost of the sealing cover can be reduced, and the material cost of the whole anti-pollution nucleic acid detection device is reduced.
Above-mentioned anti-pollution nucleic acid detection device sets up the puncture piece and is located the part salient in the groove of stepping down makes a groove's notch, and the operator of convenience presses, because the outstanding part of puncture piece forms the convex part for sealed lid, the operator easily touches the puncture piece, accurate application of force.
Above-mentioned anti-pollution nucleic acid detection device is located at the puncture piece the part in groove of stepping down and being located part in the interface channel all is provided with the locating part, can prevent effectively that the puncture piece from pressing excessively, avoids the puncture piece to drop from the closing cap simultaneously, and the setting of locating part has effectively restricted the home range of puncture piece for the sealing member is at its puncture within range activity.
Above-mentioned anti-pollution nucleic acid detection device is in the puncture piece orientation tip in the interface channel is sharp column structure, the puncture of being convenient for, and the sealed subassembly can be punctured fast to sharp column structure, and the user need not excessive application of force, only needs gently one to press can realize sealed subassembly's puncture.
Above-mentioned anti-pollution nucleic acid detection device sets up seal assembly and is aluminium membrane, plastic film, plastic plug or rubber silica gel stopper, and is sealed effectual on the one hand, and is with low costs, and on the other hand, the puncture is easy, and the user need not excessive application of force, only needs gently one to press and can realize seal assembly's puncture.
Above-mentioned anti-pollution nucleic acid testing device will be a plurality of the reagent storehouse distribute in around the reaction storehouse, the reaction storehouse is located connect one side in storehouse, the reagent storehouse is located connect the opposite side in storehouse, promoted whole anti-pollution nucleic acid testing device's structure harmony.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the application, and that other drawings can be derived from these drawings by a person skilled in the art without inventive effort.
For a more complete understanding of the present application and its advantages, reference is now made to the following descriptions taken in conjunction with the accompanying drawings. Wherein like reference numerals refer to like parts in the following description.
FIG. 1 is a schematic view of an antipollution nucleic acid detecting apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic cross-sectional view of a nucleic acid detecting apparatus according to an embodiment of the present invention.
Description of the reference numerals
10. An anti-contamination nucleic acid detection device; 100. a reagent bin; 101. a reagent storage chamber; 102. the opening of the reagent bin; 200. a reaction bin; 201. a reaction chamber; 300. a connecting bin; 301. a connecting channel; 400. a seal assembly; 500. a sealing cover; 510. a yielding groove; 600. a piercing member; 710. and a limiting member.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The embodiment of the application provides an anti-pollution nucleic acid detection device 10 to solve the problems that the pollution to the environment and the reaction tube is easily caused by repeatedly opening the reaction tube in the traditional nucleic acid detection method, the operation process is complicated, and the detection efficiency is low. The following description will be made with reference to the accompanying drawings.
The contamination-preventive nucleic acid detecting device 10 provided by the embodiment of the present application is exemplarily shown in fig. 1, and fig. 1 is a schematic structural view of the contamination-preventive nucleic acid detecting device 10 provided by the embodiment of the present application. The contamination-preventive nucleic acid detecting device 10 of the present application can be used for nucleic acid detection.
In order to more clearly explain the structure of the contamination-preventive nucleic acid detecting apparatus 10, the contamination-preventive nucleic acid detecting apparatus 10 will be described below with reference to the drawings.
Referring to fig. 1 and 2, fig. 1 is a schematic structural view of a contamination-preventive nucleic acid detecting apparatus 10 according to an embodiment of the present invention. FIG. 2 is a schematic cross-sectional view of the contamination-preventive nucleic acid detecting apparatus 10 according to one embodiment of the present invention. Illustratively, a contamination-preventive nucleic acid detecting device 10 includes a reagent cartridge 100, a reaction cartridge 200, and a connecting cartridge 300.
The reagent cartridge 100 is provided with a plurality of reagent cartridges 100, each reagent cartridge 100 is provided with a reagent storage cavity 101 and a reagent cartridge opening 102 communicated with the reagent storage cavity 101, a sealing assembly 400 is connected to each reagent cartridge 100, and each sealing assembly 400 is used for sealing the reagent cartridge opening 102. Referring to fig. 1, the number of reagent chambers 100 is four. It will be appreciated that in other embodiments, the number of reagent cartridges 100 may be other numbers, such as two, three, etc.
The reaction chamber 200 has a reaction chamber 201 for storing a base reagent. Different kinds of reagents may be stored in different reaction chambers 200, or the same reagent may be stored in a part of the reaction chambers 200. Specifically, the storage type of the reaction chamber 200 can be selected according to actual needs.
Each reagent cartridge 100 is provided with a connecting cartridge 300. The connecting bin 300 has a connecting channel 301. The connection channel 301 communicates with the reaction chamber 201. The connecting channel 301 also extends to the sealing assembly 400, and the cartridge 300 has the piercing member 600 slidably attached thereto. The piercing member 600 is used to pierce the sealing assembly 400 to enable the connecting channel to communicate with the reagent storage chamber 101.
In one embodiment, the connection cartridge 300 is mounted at the reagent cartridge opening 102 and covers the seal assembly 400.
In one embodiment, referring to fig. 1, a plurality of connecting chambers 300 are connected to form an integrated structure. The anti-pollution nucleic acid detecting device 10 connects the plurality of connecting bins 300 to form an integrated structure, so that the structure of the kit is more compact.
In one embodiment, referring to fig. 1 and 2, the connecting bin 300 has connecting slots. The connecting bin 300 is detachably connected with a sealing cover 500. The sealing cover 500 is connected to the connecting bin 300 and forms a connecting channel 301 with the connecting groove, and the puncturing element 600 penetrates through the sealing cover 500 and forms a dynamic seal with the sealing cover 500. The anti-pollution nucleic acid detecting device 10 is provided with the sealing cover 500 which is detachably connected, so that the device is convenient to disassemble.
In one embodiment, the sealing cover 500 may be inserted into a notch of the coupling groove. In another embodiment, the sealing cover 500 may be further screw-coupled to a notch of the coupling groove.
In one embodiment, as shown in fig. 1 and 2, a concave relief groove 510 is formed on an outer surface of the sealing cover 500. The piercing member 600 is partially located in the relief groove 510 and partially penetrates through the groove bottom surface of the relief groove 510 and extends into the connecting channel 301. The anti-contamination nucleic acid detecting device 10 is provided with the recessed recess 510 on the sealing cover 500, and the piercing member 600 is partially located in the recess 510, and the piercing member 600 is partially accommodated in the recess 510, thereby reducing the area of the piercing member 600 exposed to the outside, and the recess 510 can also effectively protect the piercing member 600. In addition, the provision of the avoiding groove 510 in the sealing cover 500 can reduce the material cost of the sealing cover 500 and the whole contamination-preventive nucleic acid detecting apparatus 10.
In one embodiment, as shown in fig. 1 and 2, the portion of the piercing element 600 located in the relief groove 510 protrudes from the notch of the relief groove 510. The anti-pollution nucleic acid detecting device 10 is provided with the portion of the puncture piece 600 located in the receding groove 510 protruding out of the notch of the receding groove 510, so that the operator can press the device conveniently, and the portion of the puncture piece 600 protruding out forms a convex portion relative to the sealing cover 500, so that the operator can easily touch the puncture piece 600 and apply force accurately.
In one embodiment, the sealing cover 500 may be made of a rubber material or a silicone material. The sealing cover 500 made of rubber material or silica gel material can improve the sealing effect between the sealing cover and the notch of the receding groove 510, and avoid affecting the sealing performance of the connecting channel 301.
In one embodiment, referring to fig. 2, the portion of the piercing member 600 located in the relief groove 510 and the portion located in the connecting channel 301 are connected to a stopper 710. Stopper 710 protrudes from the outer peripheral surface of piercing member 600. The above-mentioned anti-pollution nucleic acid detecting device 10 is provided with the stopper 710 at the portion of the piercing member 600 located in the avoiding groove 510 and the portion located in the connecting channel 301, and can effectively prevent the piercing member 600 from being pressed excessively, and simultaneously avoid the piercing member 600 from falling off from the sealing cover 500, and the stopper 710 effectively limits the range of motion of the piercing member 600, so that the sealing member moves in the range of piercing thereof.
In one embodiment, as shown in FIG. 2, the end of piercing element 600 facing into connecting channel 301 is pointed. Above-mentioned anti-pollution nucleic acid detecting device 10 is sharp column structure towards the tip in the interface channel 301 at puncture piece 600, the puncture of being convenient for, and the sealed subassembly 400 can be punctured fast to sharp column structure, and the user need not excessive application of force, only needs gently one to press the puncture that can realize sealed subassembly 400.
In one embodiment, the sealing member 400 is an aluminum film, a plastic plug, or a rubber silicone plug. The seal assembly 400 is not limited to the above, and the seal assembly 400 may have other configurations, which are not exhaustive. Above-mentioned anti-pollution nucleic acid detecting device 10 sets up seal assembly 400 and is aluminium membrane, plastic film, plastic plug or rubber silica gel stopper, and is sealed effectual on the one hand, and is with low costs, and on the other hand, the puncture is easy, and the user need not excessive application of force, only needs gently one to press the puncture that can realize seal assembly 400. For example, in one particular embodiment, the seal assembly 400 is an aluminum film. In another embodiment, the seal assembly 400 is a plastic film.
In one embodiment, referring to fig. 1, a plurality of reagent chambers 100 are distributed around the reaction chamber 200, the reaction chamber 200 is located at one side of the connection chamber 300, and the reagent chamber 100 is located at the other side of the connection chamber 300. Above-mentioned anti-pollution nucleic acid testing device 10 distributes a plurality of reagent storehouses 100 around reaction storehouse 200, and reaction storehouse 200 is located the one side of connecting storehouse 300, and reagent storehouse 100 is located the opposite side of connecting storehouse 300, has promoted whole anti-pollution nucleic acid testing device 10's structure harmony.
Referring to fig. 1, fig. 1 shows four reagent chambers 100, the four reagent chambers 100 are distributed in two rows and two columns, four connecting chambers 300 on the four reagent chambers 100 are distributed in two rows and two columns, the four connecting chambers 300 are located at the same height, and the reaction chamber 200 is connected to the center positions of the four connecting chambers 300, when machining is performed, the reagent chambers 100 and the corresponding connecting chambers 300 can be integrally formed, the four sealing covers 500 and the reaction chamber 200 can be integrally formed, and the connecting chambers 300 can be connected to the corresponding sealing covers 500.
The working principle of the contamination-preventive nucleic acid detecting apparatus 10 described above is as follows:
the liquid reagent 1, the solid reagent 1, the liquid reagent 2, and the liquid reagent 3 are stored in the reagent cartridge 100. Wherein, the liquid reagent 1 can be selected from magnesium acetate, the solid reagent 1 can be selected from freeze-dried powder, and the components of the freeze-dried powder are as follows: hot start Taq enzyme + dNTPs. The liquid reagent 2 is a reaction buffer. Liquid reagent 3 is template and primer. When the reagent in the reagent cartridge 100 needs to be added, the corresponding puncture piece 600 needs to be pressed. The reagents in the respective reagent cartridges 100 do not interfere with each other.
Above-mentioned anti-pollution nucleic acid detection device 10 can independently keep multiple reagent respectively in each reagent storehouse 100, and the reagent in each reagent storehouse 100 can not influence each other, and in the amplification process after injecting the nucleic acid extract, can realize need not to open the reaction tube, and after the nucleic acid amplification reaction is accomplished, open the reaction tube again and transfer the amplification product to the test paper strip from the reaction tube, through the visual detection of transparent window to judge and read the result. Because the reaction tube does not need to be opened in the amplification process after the nucleic acid extracting solution is injected, the environment cannot be polluted, and the pollution of the amplification product to the environment is avoided. In addition, the operation process is reduced without opening the reaction tube in the amplification process, and the detection efficiency is improved. In addition, the operation process is reduced without opening the reaction tube in the amplification process, and the detection efficiency is improved. Above-mentioned anti-pollution nucleic acid detection device 10 is when using, after adding the nucleic acid extract product, the action of subsequent addition reagent to need press puncture piece 600 can, puncture piece 600 puncture corresponding seal assembly 400 after for the reagent chamber and the connection intercommunication in corresponding reagent storehouse 100, corresponding reagent can be through connecting channel 301 entering reaction chamber 201 in, need not to open reaction storehouse 200, the whole in-process only need press puncture piece 600 can.
In the foregoing embodiments, the descriptions of the respective embodiments have respective emphasis, and for parts that are not described in detail in a certain embodiment, reference may be made to related descriptions of other embodiments.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. An antipollution nucleic acid detecting device, comprising:
the reagent storage device comprises a plurality of reagent bins, a plurality of sealing assemblies and a plurality of control modules, wherein each reagent bin is provided with a reagent storage cavity and a reagent bin opening communicated with the reagent storage cavity, and each reagent bin is connected with one sealing assembly which is used for sealing the reagent bin opening;
the reaction bin is provided with a reaction cavity for storing a basic reagent; and
connect the storehouse, each install respectively on the reagent storehouse connect the storehouse, connect the storehouse and have the interface channel, the interface channel with the reaction chamber intercommunication, the interface channel still extends to seal assembly, sliding connection has the puncture piece on the connection storehouse, the puncture piece is used for the puncture seal assembly is in order to realize the interface channel with the chamber intercommunication is stored to the reagent.
2. The contamination-preventive nucleic acid detecting device according to claim 1, wherein the connection cartridge is mounted at the reagent cartridge opening and covers the seal assembly.
3. The contamination-preventive nucleic acid detecting apparatus according to claim 1, wherein a plurality of the connecting chambers are connected in an integrated structure.
4. The device according to claim 1, wherein said connecting chamber has a connecting groove, said connecting chamber is detachably connected with a sealing cover, said sealing cover is connected to said connecting chamber and forms said connecting channel with said connecting groove, said piercing member is inserted into said sealing cover and forms a dynamic seal with said sealing cover.
5. The device according to claim 4, wherein a recessed groove is provided on the outer surface of said sealing cap, and said piercing member is partially located in said groove and partially penetrates the bottom surface of said groove and extends into said connecting channel.
6. The contamination-preventive nucleic acid detecting apparatus according to claim 5, wherein a portion of the piercing member located in the avoiding groove protrudes from a notch of the avoiding groove.
7. The contamination-preventive nucleic acid detecting apparatus according to claim 5, wherein a stopper is attached to each of the portion of the piercing member located in the receding groove and the portion located in the connecting channel, the stopper protruding from an outer peripheral surface of the piercing member.
8. The contamination-preventive nucleic acid detecting apparatus according to any one of claims 1 to 7, wherein the end of the piercing member facing into the connecting channel is in a pointed structure.
9. The contamination-preventive nucleic acid detecting device according to any one of claims 1 to 7, wherein the sealing member is an aluminum film, a plastic plug or a rubber silica gel plug.
10. The device according to any one of claims 1 to 7, wherein a plurality of said reagent compartments are arranged around said reaction compartment, said reaction compartment being located on one side of said connecting compartment and said reagent compartment being located on the other side of said connecting compartment.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110732456.7A CN113462538A (en) | 2021-06-29 | 2021-06-29 | Anti-pollution nucleic acid detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110732456.7A CN113462538A (en) | 2021-06-29 | 2021-06-29 | Anti-pollution nucleic acid detection device |
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| CN202110732456.7A Pending CN113462538A (en) | 2021-06-29 | 2021-06-29 | Anti-pollution nucleic acid detection device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113897277A (en) * | 2021-10-15 | 2022-01-07 | 圣湘生物科技股份有限公司 | Reaction apparatus and control method thereof |
| CN114700128A (en) * | 2022-06-07 | 2022-07-05 | 至美时代生物智能科技(北京)有限公司 | Micro-fluidic chip |
-
2021
- 2021-06-29 CN CN202110732456.7A patent/CN113462538A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113897277A (en) * | 2021-10-15 | 2022-01-07 | 圣湘生物科技股份有限公司 | Reaction apparatus and control method thereof |
| CN113897277B (en) * | 2021-10-15 | 2023-12-01 | 圣湘生物科技股份有限公司 | Reaction apparatus and control method thereof |
| CN114700128A (en) * | 2022-06-07 | 2022-07-05 | 至美时代生物智能科技(北京)有限公司 | Micro-fluidic chip |
| CN114700128B (en) * | 2022-06-07 | 2022-08-12 | 至美时代生物智能科技(北京)有限公司 | Micro-fluidic chip |
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