CN211235556U

CN211235556U - Rapid detection kit for dopamine in biological sample

Info

Publication number: CN211235556U
Application number: CN201922347254.1U
Authority: CN
Inventors: 刘士蒙; 吕昌银
Original assignee: Nanhua University
Current assignee: Nanhua University
Priority date: 2019-12-24
Filing date: 2019-12-24
Publication date: 2020-08-11
Anticipated expiration: 2029-12-24

Abstract

The utility model discloses a short-term test kit of dopamine among biological sample, comprises a box bod, one side of box body upper end articulates there is the lid, one side embedding that the box body up end is close to articulated department has the color comparison dish, it has the colorimetric range that one row of equidistance distributes to distribute on the color comparison dish, the standing groove that three equidistance distributes is seted up to one side that the color comparison dish was kept away from to the box body up end, and is three place NaAc-HAc buffer liquid bottle, TMB solution reagent bottle and Bromelain-MnO in respectively in the standing groove₂The middle part of the upper end face of the box body and one side close to the colorimetric disc are provided with a placing seat hole, a detection box is placed in the placing seat hole, the upper end face of the detection box is provided with a plurality of detection slotted holes distributed at equal intervals, and the upper end face of the detection box is provided with a plurality of detection slotted holes distributed at equal intervalsOne side that detects box up end and be close to the standing groove articulates there is the lid. The utility model discloses easy and simple to handle, detect fast, use cost low in the use, be favorable to popularizing and applying.

Description

Rapid detection kit for dopamine in biological sample

Technical Field

The utility model relates to a dopamine detects technical field, especially relates to a rapid detection kit of dopamine in biological sample.

Background

Dopamine (DA), which is a direct product of dopa decarboxylase acting on dopa, is metabolized into tyrosine-levodopa-DA-norepinephrine, and finally, catecholamine oxidized methyl translocase and monoamine oxidase are subjected to enzymatic hydrolysis to lose activity. Is an indispensable nerve conduction substance in human body and is widely existed in mammal body. Has important roles in human functional metabolism, cardiovascular system, central nervous system, kidney system and endocrine system. If the concentration of dopamine in the brain is abnormal, mental diseases such as Parkinson's disease and schizophrenia can be caused. At present, many methods for detecting dopamine content have been reported, such as electrochemical method, high performance liquid chromatography, fluorescence spectroscopy, capillary electrophoresis, and the like. These methods have the disadvantages of high sensitivity and high specificity, but also require expensive instruments, are expensive, complicated in procedure, and long in time. In order to meet the increasing requirements of dopamine detection in the fields of clinical diagnosis, biological analysis and the like, a kit for rapidly detecting dopamine in a biological sample is provided.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a rapid detection kit for dopamine in a biological sample.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a quick detect reagent box of dopamine in biological sample, includes the box body, one side of box body upper end articulates there is the lid, one side embedding that the box body up end is close to articulated department has the color comparison dish, it has the colorimetric range that a row of equidistance distributes to distribute on the color comparison dish, the one side that the color comparison dish was kept away from to the box body up end is seted up the standing groove that three equidistance distributes, and is three place NaAc-HAc buffer liquid bottle, TMB solution reagent bottle and Bromelain-MnO in the standing groove respectively₂The solution bottle, the middle part of box body up end and be close to one side of colour comparison dish and seted up and place the seat hole, place the downthehole detection box that has placed of seat, the up end of detection box sets up the detection slotted hole that a plurality of equidistance distribute, one side that the up end of detection box is close to the standing groove articulates there is the lid.

Preferably, the sucking discs are installed at four corners of the outer lower surface of the box body, the lower surfaces of two ends of one side, away from the hinged position, of the box cover are provided with cover closing heads, two ends, away from one side of the hinged position, of the upper end face of the box body correspond to the cover closing heads and are provided with cover closing holes, and the middle of the lower surface of one side, away from the hinged position, of the box cover is provided with an opening groove.

Preferably, the colorimetric regions are blue, and the blue colors between the colorimetric regions are sequentially arranged from deep to light.

Preferably, the NaAc-HAc buffer solution bottle is filled with NaAc-HAc buffer solution, the TMB solution reagent bottle is filled with TMB solution, and the Bromelain-MnO is filled with the TMB solution₂Bromelain-MnO is contained in the solution bottle₂And (3) solution.

Preferably, the upper part of the box body corresponding to the placing groove is provided with a limiting rubber block.

Preferably, when the cover body and the detection box are closed, the upper surface of the cover body is flush with the upper end face of the box body, and the accommodating groove is formed in the inner surface of the box cover corresponding to the cover body.

The utility model provides a quick detect reagent box of dopamine among biological sample, beneficial effect lies in: in the using process, the scheme is to put a NaAc-HAc buffer solution bottle, a TMB solution reagent bottle and a Bromelain-MnO₂The corresponding liquid in the solution bottle is quantitatively injected into the detection slot, the biological sample which needs to be subjected to dopamine detection after pretreatment is placed in the detection slot, the color of the solution in the detection slot is further observed and compared with the colorimetric area in the colorimetric disc, the detection of the dopamine and the dopamine content in the biological sample can be realized through comparison, and the whole kit is simple and convenient to operate, rapid in detection and low in use cost in the use process, and is favorable for popularization and application.

Drawings

Fig. 1 is a top view of the open lid of a rapid dopamine detection kit for biological samples according to the present invention;

fig. 2 is a front view of the fast dopamine detecting kit for biological samples according to the present invention with an open lid;

fig. 3 is a front view of the rapid dopamine detection kit for biological samples according to the present invention with a closed lid;

fig. 4 is a top view of a case body of a rapid dopamine detection kit for a biological sample according to the present invention;

fig. 5 is a front view of a detection box of the rapid dopamine detection kit for biological samples according to the present invention.

In the figure: the kit comprises a kit body 1, a covering hole 101, a sucker 102, a kit cover 2, a covering head 201, an opening groove 202, a placing groove 3, a NaAc-HAc buffer solution bottle 301, a TMB solution reagent bottle 302 and Bromelain-MnO₂ Solution bottle 303, place seat hole 4, detect box 5, detect slotted hole 501, lid 6, color comparison dish 7, than color zone 8, spacing rubber piece 9, holding tank 10.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-5, a rapid detection kit for dopamine in biological samples comprises a box body 1, a box cover 2 is hinged to one side of the upper end of the box body 1, a colorimetric disc 7 is embedded into one side, close to the hinged part, of the upper end face of the box body 1, a row of colorimetric areas 8 are distributed on the colorimetric disc 7 and are distributed at equal intervals, the colorimetric areas 8 are blue, and the blue between the colorimetric areas 8 are sequentially distributed from deep to light.

Three equidistantly distributed placing devices are arranged on one side of the upper end surface of the box body 1 away from the colorimetric disc 7A NaAc-HAc buffer solution bottle 301, a TMB solution reagent bottle 302 and Bromelain-MnO are respectively arranged in the three placing grooves 3₂The solution bottle 303, the NaAc-HAc buffer solution bottle 301 are filled with NaAc-HAc buffer solution, the TMB solution reagent bottle 302 is filled with TMB solution and Bromelain-MnO₂Bromelain-MnO is contained in the solution bottle 303₂The pH value of the concentration of NaAc-HAc buffer solution is 4.75, and the concentration is 100 mmol/L; the concentration of the TMB solution is 5 mmol/L; Bromelain-MnO₂The concentration of the solution was 5. mu. mol/L.

The middle part of 1 up end of box body and the one side that is close to colorimetric disc 7 have been seted up and have been placed seat hole 4, place and have placed detection box 5 in the seat hole 4, the up end of detection box 5 sets up the detection slotted hole 501 that a plurality of equidistance distribute, one side that detection box 5 up end is close to standing groove 3 articulates there is lid 6, lid 6 and detection box 5 are fashionable to cover, the upper surface of lid 6 and the up end looks parallel and level of box body 1, and the internal surface of lid 2 corresponds lid 6 and has seted up holding tank 10, it can follow and place seat hole 4 and go up dismantlement to detect box 5 in the in-process that uses, be convenient for clear up after the dismantlement, detect box 5 relatively at lid 6 and cover and close and lid 2 relative box body 1 carries out under the state that covers and close, holding tank 10 can hold.

Sucking disc 102 is installed in the four corners of the outside lower surface of box body 1, the lower surface at the both ends of keeping away from one side of articulated department on lid 2 is equipped with the lid and closes head 201, the both ends that articulated department one side was kept away from to 1 up end of box body correspond the lid and close head 201 and seted up lid and close hole 101, keep away from the middle part of articulated department one side lower surface on lid 2 and seted up opening groove 202, under the state that the kit does not use, lid 2 is the lid and closes the state for box body 1, lid is closed first 201 card and is established in lid and closes hole 101 this moment, and then can make lid between lid 2 and the box body 1 close and be the steady state, sucking disc 102's setting can make the kit present the steady.

The upper part of the box body 1 corresponding to the placing groove 3 is provided with a limiting rubber block 9, and when a NaAc-HAc buffer solution bottle 301, a TMB solution reagent bottle 302 and Bromelain-MnO are taken as the components₂When the solution bottle 303 is placed in the corresponding placing groove 3, the limit rubber block 9 can be used for corresponding NaAc-HAc buffer solution bottle 301, TMB solution reagent bottle 302 and Bromelain-MnO₂The solution bottle 303 is fixed and limited, so that a NaAc-HAc buffer solution bottle 301, a TMB solution reagent bottle 302 and Bromelain-MnO are further enabled to be arranged₂The solution bottle 303 assumes a stable state in the placement tank 3.

In summary, the following steps: the utility model discloses in the in-process of using for with NaAc-HAc buffer solution bottle 301, TMB solution reagent bottle 302, Bromelain-MnO₂The corresponding liquid in the solution bottle 303 is quantitatively injected into the detection slot 501, and Bromelain-MnO is added under the injection of NaAc-HAc buffer solution₂The solution can catalyze 3,3 ', 5, 5' -tetramethylbenzidine in the TMB solution to generate a blue oxTMB product, the mixed solution in the detection slot 501 is blue, the pretreated biological sample needing dopamine detection is placed in the detection slot 501, and the dopamine in the biological sample can catalyze MnO in the mixed solution₂Reduction to Mn²⁺Ions, thereby making Bromelain-MnO₂The catalytic effect of the solution disappears, namely the solution in the detection slot 501 changes color, the color of the solution in the detection slot 501 is further observed and compared with the colorimetric area 8 in the colorimetric disc 7, the judgment of the dopamine in the biological sample and the semi-quantitative detection of the dopamine content can be realized through the comparison, and the whole kit is simple and convenient to operate, quick to detect and low in use cost in the use process, and is favorable for popularization and application.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a quick detect reagent box of dopamine among biological sample, includes box body (1), one side of box body (1) upper end articulates there is lid (2), its characterized in that, one side embedding that box body (1) up end is close to articulated department has colorimetric disc (7), it has color comparison district (8) that one row of equidistance distributes to distribute on colorimetric disc (7), box body (1) up endOne side far away from the colorimetric disc (7) is provided with three placing grooves (3) which are distributed at equal intervals, and three NaAc-HAc buffer solution bottles (301), TMB solution reagent bottles (302) and Bromelain-MnO are respectively placed in the placing grooves (3)₂Solution bottle (303), the middle part of box body (1) up end and the one side of being close to colorimetric disc (7) have been seted up and have been placed seat hole (4), place and have been placed detection box (5) in seat hole (4), the up end of detection box (5) is seted up detection slotted hole (501) that a plurality of equidistance distribute, one side that detection box (5) up end is close to standing groove (3) articulates there is lid (6).

2. The kit for rapidly detecting dopamine in a biological sample according to claim 1, wherein four corners of the outer lower surface of the box body (1) are provided with suckers (102), the lower surfaces of two ends of one side of the box cover (2) far away from the hinge joint are provided with cover heads (201), two ends of one side of the upper end surface of the box body (1) far away from the hinge joint are provided with cover holes (101) corresponding to the cover heads (201), and the middle part of the lower surface of one side of the box cover (2) far away from the hinge joint is provided with an opening groove (202).

3. The kit for rapidly detecting dopamine in biological samples according to claim 1, wherein the colorimetric regions (8) are blue, and the blue colors between the colorimetric regions (8) are sequentially arranged from deep to light.

4. The kit for rapidly detecting dopamine in biological samples according to claim 1, wherein the NaAc-HAc buffer solution bottle (301) contains NaAc-HAc buffer solution, the TMB solution reagent bottle (302) contains TMB solution, and the Bromelain-MnO is₂Bromelain-MnO is contained in the solution bottle (303)₂And (3) solution.

5. The kit for rapidly detecting dopamine in a biological sample according to claim 1, wherein a limiting rubber block (9) is arranged on the upper portion of the portion, corresponding to the placement groove (3), of the box body (1).

6. The kit for rapidly detecting dopamine in a biological sample according to claim 1, wherein when the cover body (6) and the detection box (5) are covered, the upper surface of the cover body (6) is flush with the upper end surface of the box body (1), and the inner surface of the box cover (2) is provided with a containing groove (10) corresponding to the cover body (6).