CN212301398U

CN212301398U - Exosome protein concentration detection device

Info

Publication number: CN212301398U
Application number: CN202020804434.8U
Authority: CN
Inventors: 董鸣; 何慧琼; 沈通
Original assignee: Nanjing Qiyi Technology Co ltd
Current assignee: Nanjing Qiyi Technology Co ltd
Priority date: 2020-05-14
Filing date: 2020-05-14
Publication date: 2021-01-05
Anticipated expiration: 2030-05-14

Abstract

The utility model relates to an exosome protein concentration detection technical field just discloses an exosome protein concentration detection device, including magnetic force-electrochemical sensor box, the front side activity of magnetic force-electrochemical sensor box is pegged graft and is had the sample storage pool, the connecting pipe has been seted up to the front side surface in sample storage pool, it has the sample to keep in the pipe to peg graft in the inside of connecting pipe, the upper portion fixed mounting that the sample kept in the pipe has break-make switch one, the top fixedly connected with connecting seat that the sample kept in the pipe, the top fixed connection of connecting seat has horizontal reagent pipe. Through the intercommunication between the sample pipe of keeping in of slip piston control horizontal reagent pipe and each part, after all filling the exosome that waits to detect in every sample pipe of keeping in, push back piston to on-off switch two places, open all on-off switch one simultaneously this moment, can realize getting into the exosome that the sample inslot portion detected and get into simultaneously with measuring to reach the effect that synchronous with measuring the sample assurance detection precision.

Description

Exosome protein concentration detection device

Technical Field

The utility model relates to an exosome protein concentration detection technical field specifically is an exosome protein concentration detection device.

Background

The exosome is an extracellular nanoscale vesicle formed by cells through a series of regulation processes such as endocytosis, fusion and efflux, and the diameter of the vesicle is about fifty to one hundred fifty nanometers. Exosomes can carry proteins, transport RNA, and play important roles in intercellular substance and information transduction. Research shows that the exosome protein is expressed in a large amount in exosomes of pancreatic cancer and breast cancer cells, and the content of the exosome protein is in direct proportion to the size of a tumor. The exosome protein detection can be used for clinical cancer diagnosis and treatment monitoring, and has important significance, the exosome protein can be detected by combining an immunomagnetic bead technology and an electrochemical sensor detection technology at present, but the addition process of a sample cannot achieve synchronous and same-amount addition of the sample when a magnetic-electrochemical sensor detects, so that the detected protein concentration is not accurate enough.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The not enough to prior art, the utility model provides an exosome protein concentration detection device possesses synchronous same volume and adds the sample and guarantee to detect the precision advantage, has solved current detection method and can not realize synchronous same volume and add the problem that the sample guaranteed to detect the precision.

(II) technical scheme

For realizing above-mentioned synchronous same amount of interpolation sample assurance detection precision purpose, the utility model provides a following technical scheme: the exosome protein concentration detection device comprises a magnetic force-electrochemical sensor box body, wherein a sample storage pool is movably inserted into the front side of the magnetic force-electrochemical sensor box body, a connecting pipe is arranged on the front side surface of the sample storage pool, a sample temporary storage pipe is inserted into the connecting pipe, a first on-off switch is fixedly mounted on the upper portion of the sample temporary storage pipe, a connecting seat is fixedly connected to the top of the sample temporary storage pipe, a horizontal reagent pipe is fixedly connected to the top of the connecting seat, a piston is movably connected to the inner wall of the horizontal reagent pipe, a push rod is fixedly connected to the right end of the piston, a baffle disc is fixedly connected to the right end of the push rod, a second on-off switch is fixedly connected to the left end of the horizontal reagent pipe, a liquid flow pipe is fixedly connected to the left end of the second, the left end top fixedly connected with support of magnetic force-electrochemical sensor box, the top fixedly connected with solution bucket of support, the top fixedly connected with motor of solution bucket, the bottom fixedly connected with of motor rotates the post, the outside fixedly connected with stirring rod that rotates the post, the observation window has been seted up to the left side of solution bucket fixedly, the left end front side fixed mounting of magnetic force-electrochemical sensor box has the ampere meter, the right side fixed mounting of magnetic force-electrochemical sensor box has the data line interface, the louvre has been seted up to the magnetic force-electrochemical sensor box side at data line interface top, the inside top fixedly connected with sample cell in sample storage pool.

Preferably, the magnetic-electrochemical sensor box is externally connected with a power supply for power supply, the right side of the magnetic-electrochemical sensor box is in signal connection with a computer through a data line interface, and a detection result generated by the magnetic-electrochemical sensor box is displayed through the computer connected to the right end of the data line interface.

Preferably, the sample storage pool is of a pull type, the temporary sample storage tube is hermetically connected with the connecting tube through a movable connecting mechanism, the bottom of the temporary sample storage tube is of a right-angle bent shape with a curved surface, and the on-off switch I controls the on-off of the temporary sample storage tube.

Preferably, the piston is made of rubber, the outer wall of the piston is in tight sliding connection with the inner wall of the horizontal reagent tube, and the length of the push rod is equal to that of the inner tube wall part of the horizontal reagent tube.

Preferably, a buffer gasket is arranged between the bottom of the bracket and the top of the magnetic-electrochemical sensor box, and the width of the magnetic-electrochemical sensor box at the position where the bracket is arranged on the horizontal plane is longer than that of the magnetic-electrochemical sensor box at the right end of the bracket.

Preferably, the front end of the sample cell is fixedly communicated with the rear end of the connecting pipe, the rear end of the sample cell is respectively connected with the positive electrode and the negative electrode which are connected with a power supply through electric leads, and the interior of the sample cell is hermetically connected.

(III) advantageous effects

Compared with the prior art, the utility model provides an exosome protein concentration detection device possesses following beneficial effect:

1. this exosome protein concentration detection device, place inside the solution bucket through the exosome sample that will handle, through two intercommunication liquid flow tubes of on-off switch and horizontal reagent pipe, it is inside to make the exosome that treats detects to pass through on-off switch two by liquid flow tube and vertical connecting pipe entering horizontal reagent intraduct, then keep in the intercommunication between the pipe through the sample of sliding piston control horizontal reagent pipe and each part, keep in the pipe and all fill in every sample and wait to detect the exosome after, the back pushes away piston to on-off switch two departments, open all on-off switch one simultaneously this moment, can realize getting into the exosome that the inside detection of sample cell was measured simultaneously with quantitative entering, thereby reach the effect that synchronous same volume adds the sample and guarantee to detect the precision.

2. This exosome protein concentration detection device, through keeping in the pipe with the sample and designing into right angle bending form, then keep in pipe and connecting pipe through swing joint mechanism connection sample, install horizontal reagent pipe in the position far away of the front side of magnetic force-electrochemical sensor box, make between its and the magnetic force-electrochemical sensor box interval one end distance, when needs are cleaned sample storage pool inside, only need keep in the pipe with the sample and dismantle, can realize the sample and keep in the separation between pipe and the sample storage pool, thereby reached and carried out convenient clear effect to sample storage pool inside.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic top view of the present invention;

FIG. 3 is a schematic view of the structure of the sample of the present invention;

fig. 4 is a schematic side view of the present invention.

In the figure: the device comprises a magnetic force-electrochemical sensor box body, a 2-sample storage pool, a 3-connecting pipe, a 4-sample temporary storage pipe, a 5-on-off switch I, a 6-connecting seat, a 7-horizontal reagent pipe, an 8-piston, a 9-push rod, a 10-baffle disc, an 11-on-off switch II, a 12-fluid pipe, a 13-vertical connecting pipe, a 14-bracket, a 15-solution barrel, a 16-motor, a 17-rotating column, an 18-stirring rod, a 19-observation window, a 20-ammeter, a 21-data line interface, a 22-heat dissipation hole and a 23-sample tank.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, an exosome protein concentration detection device comprises a magnetic-electrochemical sensor box 1, wherein the magnetic-electrochemical sensor box 1 is externally connected with a power supply, the right side of the magnetic-electrochemical sensor box 1 is in signal connection with a computer through a data line interface 21, and a detection result generated by the magnetic-electrochemical sensor box 1 is displayed through the computer connected to the right end of the data line interface 21. The magnetic-electrochemical sensor box body 1 belongs to electroanalytical chemical equipment, stable power supply needs to be provided in the detection process, so that the probability of faults can be reduced by directly connecting a power supply, because the volume capacity of the magnetic-electrochemical sensor box body 1 is limited, huge data signals generated by a sample groove 23 need strong computing power of a computer to analyze the data signals, and in order to reduce the volume of the magnetic-electrochemical sensor box body 1, only a computing part can be completed by an external computer. The front side of the magnetic-electrochemical sensor box body 1 is movably inserted with a sample storage pool 2, the sample storage pool 2 is of a pull type, meanwhile, the sample temporary storage tube 4 is connected with the connecting tube 3 in a sealing mode through a movable connecting mechanism, the bottom of the sample temporary storage tube 4 is in a right-angle bent shape of a curved surface, and the on-off switch I5 controls the on-off of the sample temporary storage tube 4. Because the inside sample groove 23 of sample storage pool 2 needs clean after carrying out the primary detection, so design into the pull formula with sample storage pool 2, the inside of conveniently pulling out magnetic force-electrochemical sensor box 1 with sample storage pool 2 cleans the sample groove 23 at its top, at the in-process with the outside pull of sample storage pool 2, if sample temporary storage pipe 4 can form the fender to the outer removal of sample storage pool 2 with its fixed connection, so the connection between quiet sample temporary storage pipe 4 and connecting pipe 3 designs into swing joint mechanism, convenient dismantlement. Connecting pipe 3 has been seted up to the front side surface of sample storage pool 2, it has sample to keep in pipe 4 to peg graft in the inside of connecting pipe 3, the upper portion fixed mounting that the sample kept in pipe 4 has on-off switch 5, the sample keeps in the top of pipe 4 and is passed through welded fastening and be connected with connecting seat 6, welded fastening is passed through at the top of connecting seat 6 and is connected with horizontal reagent pipe 7, the inner wall swing joint of horizontal reagent pipe 7 has piston 8, piston 8 is the rubber material, and inseparable sliding connection between piston 8's the outer wall and the inner wall of horizontal reagent pipe 7, the length of push rod 9 equals the length of the inside pipe wall part of horizontal reagent pipe. Through the slip of piston 8 on horizontal reagent pipe 7 inner wall, can control whether can receive the exosome that waits to detect from horizontal reagent pipe 7 in the sample pipe 4 of keeping in, need not all sample groove 23 all to contact the exosome when detecting occasionally, so control the break-make between horizontal reagent pipe 7 and the sample pipe 4 of keeping in through piston 8, because the gliding scope of piston 8 is the scope of the length of whole horizontal reagent pipe 7, so the requirement of the scope of the length of horizontal reagent pipe 7 needs to be satisfied to the length of push rod 9. The right end of the piston 8 is connected with a push rod 9 through welding and fixing, the right end of the push rod 9 is connected with a baffle disc 10 through welding and fixing, the left end of the horizontal reagent tube 7 is connected with an on-off switch II 11 through welding and fixing, the left end of the on-off switch II 11 is connected with a liquid flow tube 12 through welding and fixing, the left end of the liquid flow tube 12 is connected with a vertical connecting tube 13 through welding and fixing, the top of the left end of the magnetic-electrochemical sensor box 1 is connected with a support 14 through welding and fixing, a buffer gasket is installed between the bottom of the support 14 and the top of the magnetic-electrochemical sensor box 1, and the width of the magnetic-electrochemical sensor box 1 at the installation position of the support 14 on the horizontal plane is longer than that. In order to prevent the motor 16 from generating mechanical vibration to damage the housing 1 of the magnetomechanical electrochemical sensor during rotation, a buffer gasket is installed between the bottom of the bracket 14 and the top of the housing 1 of the magnetomechanical electrochemical sensor to reduce the propagation of vibration and protect the housing 1 of the magnetomechanical electrochemical sensor, and the horizontal reagent tube 7 is installed to extend to the front side of the housing 1 of the magnetomechanical electrochemical sensor, so that the installation positions of the fluid tube 12 and the vertical connecting tube 13 need to extend the width of the housing 1 of the magnetomechanical electrochemical sensor where the solution barrel 15 is installed to the front side of the other housing 1 of the magnetomechanical electrochemical sensor in order to match the change of the extended position. The top of the bracket 14 is fixedly connected with a solution barrel 15 through welding, the top of the solution barrel 15 is fixedly connected with a motor 16 through welding, the bottom of the motor 16 is fixedly connected with a rotating column 17 through welding, the outside of the rotating column 17 is fixedly connected with a stirring rod 18 through welding, the left side of the solution barrel 15 is fixedly provided with an observation window 19, the front side of the left end of the magnetic-electrochemical sensor box 1 is fixedly provided with an ammeter 20, the right side of the magnetic-electrochemical sensor box 1 is fixedly provided with a data line interface 21, the side surface of the magnetic-electrochemical sensor box 1 at the top of the data line interface 21 is provided with a radiating hole 22, the top inside of the sample storage pool 2 is fixedly connected with a sample tank 23 through welding, the front end of the sample tank 23 is fixedly communicated with the rear end of the connecting pipe 3, and the rear end of, the interior of the sample tank 23 is hermetically connected. The sample tank 23 is used for collecting exosomes to be analyzed and detected flowing through the connecting pipe 3, and then concentration data of exosome proteins is collected through a sensor in the sample tank 23 for analysis, wherein the exosomes are stored in the sample tank 23 in the process, so that the tightness of the sample tank 23 needs to be maintained, the exosomes are prevented from leaking, measured data is prevented from deviating, and the precision of the measuring result is reduced.

The working principle is that before detection, a sample to be detected is added into the solution barrel 15 for early treatment, then the on-off switch II 11 is opened to realize the connection of the passages among the solution barrel 15, the vertical connecting pipe 13, the liquid flow pipe 12 and the horizontal reagent pipe 7, then the piston 8 is made to slide to the right end of the horizontal reagent pipe 7 along the inner wall of the horizontal reagent pipe 7 by sliding the piston 8, at the moment, the sample to be detected in the horizontal reagent pipe 7 can enter the temporary sample storage pipe 4 for temporary storage, after the sample to be detected is filled in all the temporary sample storage pipes 4, the piston 8 is made to slide leftwards to the on-off switch II 11, so that the sample in each sample storage pipe 4 is at the same height for temporary storage, then the on-off switch I5 is opened simultaneously to realize the communication between the temporary sample storage pipe 4 and the connecting pipe 3, and the exosome to be detected is sent into the sample, therefore, the purpose of synchronously adding samples in the same amount to ensure the detection precision is achieved.

To sum up, this exosome protein concentration detection device, place inside solution bucket 15 through the exosome sample that will handle, communicate flow tube 12 and horizontal reagent pipe 7 through on-off switch two 11, make the exosome that detects enter horizontal reagent pipe 7 through flow tube 12 and vertical connecting pipe 13 inside through on-off switch two 11, then through the intercommunication between horizontal reagent pipe 7 and the sample temporary storage pipe 4 of each part of slip piston 8 control, after all filling up the exosome that detects in every sample temporary storage pipe 4, push back piston 8 to on-off switch two 11 departments, open all on-off switch one 5 simultaneously this moment, can realize entering 23 inside exosomes that detect of sample groove and simultaneously the same volume entering, thereby reach the effect that synchronous same volume adds the sample and guarantee the detection precision. Through keeping in the pipe 4 with the sample and designing right angle bending form, then keep in pipe 4 and connecting pipe 3 through swing joint mechanism connection sample, install horizontal reagent pipe 7 in the position far away of the front side of magnetic force-electrochemical sensor box 1, make it and magnetic force-electrochemical sensor box 1 between the one end distance of interval, when needs are cleaned 2 insides in the sample storage pool, only need keep in the pipe 4 with the sample and dismantle, can realize that the sample keeps in the separation between pipe 4 and the sample storage pool 2, thereby reached and carried out convenient clear effect to 2 insides in the sample storage pool.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an exosome protein concentration detection device, includes magnetism-electrochemical sensor box (1), its characterized in that: the sample storage pool (2) is movably inserted into the front side of the magnetic-electrochemical sensor box body (1), a connecting pipe (3) is arranged on the front side surface of the sample storage pool (2), a sample temporary storage pipe (4) is inserted into the connecting pipe (3), a first on-off switch (5) is fixedly mounted on the upper portion of the sample temporary storage pipe (4), a connecting seat (6) is fixedly connected to the top of the sample temporary storage pipe (4), a horizontal reagent pipe (7) is fixedly connected to the top of the connecting seat (6), a piston (8) is movably connected to the inner wall of the horizontal reagent pipe (7), a push rod (9) is fixedly connected to the right end of the piston (8), a baffle disc (10) is fixedly connected to the right end of the push rod (9), a second on-off switch (11) is fixedly connected to the left end of the horizontal reagent pipe (7), and a liquid flow pipe (12) is fixedly connected to, the left end of the liquid flow pipe (12) is fixedly connected with a vertical connecting pipe (13), the top of the left end of the magnetic-electrochemical sensor box body (1) is fixedly connected with a support (14), the top of the support (14) is fixedly connected with a solution barrel (15), the top of the solution barrel (15) is fixedly connected with a motor (16), the bottom of the motor (16) is fixedly connected with a rotating column (17), the outer part of the rotating column (17) is fixedly connected with a stirring rod (18), the left side of the solution barrel (15) is fixedly provided with an observation window (19), the front side of the left end of the magnetic-electrochemical sensor box body (1) is fixedly provided with an ammeter (20), the right side of the magnetic-electrochemical sensor box body (1) is fixedly provided with a data line interface (21), and the side of the magnetic-electrochemical sensor box body (1) at the top of the data line interface (21) is provided with, the top of the interior of the sample storage pool (2) is fixedly connected with a sample groove (23).

2. The apparatus for detecting the concentration of an exosome protein according to claim 1, characterized in that: the magnetic-electrochemical sensor box body (1) is externally connected with a power supply for power supply, the right side of the magnetic-electrochemical sensor box body (1) is in signal connection with a computer through a data interface, and a detection result generated by the magnetic-electrochemical sensor box body (1) is displayed through the computer connected with the right end of a data line interface (21).

3. The apparatus for detecting the concentration of an exosome protein according to claim 1, characterized in that: the sample storage pool (2) is of a drawing type, meanwhile, the sample temporary storage tube (4) is connected with the connecting tube (3) in a sealing mode through a movable connecting mechanism, the bottom of the sample temporary storage tube (4) is in a right-angle bending shape of a curved surface, and the on-off switch I (5) controls the on-off of the sample temporary storage tube (4).

4. The apparatus for detecting the concentration of an exosome protein according to claim 1, characterized in that: the piston (8) is made of rubber, the outer wall of the piston (8) is in tight sliding connection with the inner wall of the horizontal reagent tube (7), and the length of the push rod (9) is equal to that of the inner tube wall of the horizontal reagent tube (7).

5. The apparatus for detecting the concentration of an exosome protein according to claim 1, characterized in that: a buffer gasket is arranged between the bottom of the support (14) and the top of the magnetic-electrochemical sensor box body (1), and the width of the magnetic-electrochemical sensor box body (1) at the installation position of the support (14) on the horizontal plane is longer than that of the magnetic-electrochemical sensor box body (1) at the right end on the horizontal plane.

6. The apparatus for detecting the concentration of an exosome protein according to claim 1, characterized in that: the front end of the sample groove (23) is fixedly communicated with the rear end of the connecting pipe (3), the rear end of the sample groove (23) is respectively connected with the anode and the cathode which are connected with a power supply through electric leads, and the interior of the sample groove (23) is hermetically connected.