CN112611862A - Nano enzyme chromatography reagent card box - Google Patents

Abstract

The invention relates to the technical field of reagent card boxes, and discloses a nano enzyme chromatography reagent card box which comprises a card box body and a fixed groove formed in the card box body, wherein chromatography test paper is fixedly connected in the fixed groove, a buffer liquid groove, a sample groove and a mixing cavity are arranged on the card box body, a mixing mechanism is arranged in the mixing cavity, and the mixing cavity is simultaneously communicated with the buffer liquid groove, the sample groove and the fixed groove; the inside of the buffer liquid groove is detachably connected with a buffer liquid bag, a puncturing mechanism for puncturing the buffer liquid bag is arranged in the buffer liquid groove, and a driving mechanism for driving the mixing cavity to directionally flow with the buffer liquid groove, the sample groove and the fixed groove is arranged in the card box body; the card box body is detachably connected with a storage mechanism for storing and preparing color developing liquid. Puncture mechanism punctures the buffer solution package in this application for buffer solution and sample automatic mixing utilize storage mixing mechanism to prepare color development liquid simultaneously, have solved among the prior art and have utilized reagent card box to carry out the problem that the operation is complicated and the detection precision is low when nanometer enzyme immunoassay.

Description

Nano enzyme chromatography reagent card box

Technical Field

The invention relates to the technical field of reagent card boxes, in particular to a nano enzyme chromatography reagent card box.

Background

In the nano enzyme chromatographic immunoassay, firstly, a sample and a buffer solution are diluted according to a certain proportion, then the diluted sample is dripped into a sample groove on a reagent card box, and meanwhile, a color developing solution is dripped into a color developing window in the reagent card box, so that the diluted sample is contacted with chromatographic test paper in the reagent card box and develops color under the action of the color developing solution, and a detection reaction result is obtained.

In the existing reagent card box, when carrying out the immunoassay of the nano enzyme chromatography, a buffer solution is required to be manually dripped into a sample to dilute the sample, and the dilution ratio of the sample is required to be strictly controlled during the detection so as to improve the detection precision; meanwhile, in the nanoenzyme immunoassay, in order to maximize the color development effect of the color development solution, the color development solution is generally prepared in a conventional manner. Therefore, in the case of a hospital or other occasions with high detection efficiency requirements, when the color developing solution is prepared manually and the sample is diluted by using the buffer solution, the ratio of the sample to the buffer solution is easily controlled, so that the detection result is prone to be deviated; meanwhile, when the color developing solution is prepared manually, not only is the efficiency low and cannot meet the requirements of places with higher detection frequency, but also the accurate addition amount of the solution A and the solution B cannot be ensured in the preparation process, so that the color developing effect of the color developing solution formed by mixing the solution A and the solution B is poor.

Disclosure of Invention

The invention aims to provide a nano enzyme chromatography reagent card box to solve the problems of complex operation and low detection precision when the reagent card box is used for carrying out nano enzyme immunoassay in the prior art.

In order to achieve the purpose, the invention adopts the following technical scheme: a nanometer enzyme chromatography reagent card box comprises a card box body and a fixing groove formed in the card box body, wherein chromatography test paper is fixedly connected in the fixing groove, a buffer liquid groove, a sample groove and a mixing cavity are formed in the card box body, a mixing mechanism is arranged in the mixing cavity, and the mixing cavity is simultaneously communicated with the buffer liquid groove, the sample groove and the fixing groove; the inside of the buffer liquid groove is detachably connected with a buffer liquid bag, a puncturing mechanism for puncturing the buffer liquid bag is arranged in the buffer liquid groove, and a driving mechanism for driving the mixing cavity to directionally flow with the buffer liquid groove, the sample groove and the fixed groove is arranged in the card box body; the card box body is detachably connected with a storage mechanism for storing and preparing color developing liquid.

The principle of the scheme is as follows: when the nano enzyme chromatography detection is required, firstly, a sample is dripped into a sample groove, then a buffer liquid bag is placed in the buffer liquid groove, the buffer liquid bag is punctured by a puncturing mechanism, so that the buffer liquid flows into the buffer liquid groove, then the buffer liquid in the buffer liquid groove and the sample in the sample groove are driven by a driving mechanism to enter a mixing cavity, the buffer liquid and the sample entering the mixing cavity are automatically and uniformly mixed by the mixing mechanism, and a sample to be detected (hereinafter referred to as mixed liquid) after the sample and the buffer liquid are mixed is obtained; then, continuously driving the mixed solution in the mixing cavity to the fixed groove by using the driving mechanism, so that the mixed solution is contacted with the chromatography test paper, and thus completing the sample adding of the mixed solution; meanwhile, the liquid A and the liquid B which are respectively stored are uniformly mixed by using a storage mixing mechanism to obtain color development liquid, finally, the color development liquid is drained to the color development window by using a drainage mechanism, the color development liquid is contacted with the chromatographic test paper in the fixed groove, and the chromatographic test paper shows a detection color under the color development action of the color development liquid, so that a detection result is obtained.

The beneficial effect of this scheme lies in:

1. the operation is simple and convenient: compared with the prior art that the buffer solution and the sample need to be manually mixed, the operation is complex. When carrying out immunoassay in this application, only need add the sample cell with the sample, and place the buffer solution package in the buffer solution groove, can make buffer solution and sample automatic mixing to the automatic chromatography test paper department of carrying to the fixed slot in the mixed liquid that will obtain, needn't artifical manual dropwise add, whole testing process is more simple and convenient, and the efficiency of detection is also higher.

2. The detection precision can be improved: compare in prior art artifical manual mix mixed liquid with buffer solution and sample to manual will mix on liquid dropwise add to the chromatography test paper, the dilution ratio of mixed liquid is difficult to control, and the difficult accurate control of dropwise add volume when artifical manual dropwise add mixed liquid simultaneously. In this application, buffer solution is the prestore in the buffer solution package, consequently can the volume of buffer solution in the reasonable control buffer solution package, simultaneously through the size of reasonable sample groove that sets up, can be so that the proportion of buffer solution and sample obtains accurate control to it is higher to make testing result's precision.

3. Buffer solution convenient to store: in the application, the buffer solution is stored in the buffer solution bag, and the buffer solution bag is punctured by the puncturing mechanism only when detection is needed, so that on one hand, the amount of the buffer solution can be controlled by setting the capacity of the buffer solution bag, and the dilution ratio of the buffer solution and a sample can be accurately controlled in the immunodetection process; on the other hand, with buffer solution storage in the buffer solution package, can avoid buffer solution to be contaminated, can also avoid liquid evaporation in the buffer solution to influence buffer solution self concentration simultaneously to at the immunodetection in-process, can control the dilution ratio of buffer solution and sample more accurately, be favorable to promoting immunodetection's precision.

4. The composition of the color developing liquid can be accurately controlled: in the application, the liquid A and the liquid B are separately stored in the storage mixing mechanism, so that the storage amounts of the liquid A and the liquid B can be accurately controlled, the color developing liquid is accurately prepared during the chromatographic detection of the nano enzyme, and the detection accuracy is effectively improved.

5. The liquid A and the liquid B can be effectively protected: in the application, the liquid A and the liquid B are stored separately, and the liquid A and the liquid B are mixed only when detection is needed, so that the current preparation of the color developing liquid is realized, and the detection effect is better.

6. The color developing solution can be accurately dripped: compared with the prior art in which the color developing solution needs to be manually dripped, the dripping process is easy to cause the condition of leakage or inaccurate dripping, the detection effect is poor, and the color developing solution is wasted. In this application, when needs detect, be to store mixing mechanism lug connection to the card box body on, after A liquid and B liquid mix and form the color development liquid, directly utilize drainage mechanism with the drainage of color development liquid to the color development window in, ensured the accurate dropwise add of color development liquid to make the precision of testing result higher.

Preferably, as an improvement, store mixed mechanism and include storage bottle and the puncture cover of swing joint on the storage bottle, the storage bottle can be dismantled and connect on the card box body, is equipped with a liquid A storage chamber and the liquid B storage chamber that is used for separately storing A liquid and B liquid in the storage bottle, and it has the intercommunicating pore with A liquid storage chamber intercommunication to open on the liquid B storage chamber, is equipped with first sealing member on the intercommunicating pore, punctures and sheathes fixedly connected with and is used for puncturing the first point thorn of first sealing member.

In the scheme, after the storage bottle is connected to the card box body, the puncture sleeve is driven to move relative to the storage bottle by external force, so that the first sharp on the puncture sleeve punctures the first sealing element, and the liquid B stored in the liquid B storage cavity flows into the liquid A through the communication hole, so that the liquid B is mixed with the liquid A to obtain the color developing liquid.

Preferably, as an improvement, drainage mechanism includes the connecting portion of fixed connection on the card box body, it has the intercommunication chamber with the color development window intercommunication to open on the connecting portion, and it has the drainage hole with connecting portion complex to open on the A liquid storage chamber, fixedly connected with is used for the second sealing member in sealed drainage hole on the storage bottle, punctures and sheathes fixedly connected with and is used for puncturing the second point of second sealing member and stings.

According to the scheme, after the first sharp prick is used for pricking the first sealing piece to enable the liquid A and the liquid B to be mixed to form the color developing liquid, the external force is continuously used for pushing the pricking sleeve to move relative to the storage bottle, so that the second sharp prick pierces the second sealing piece, the color developing liquid flows into the communicating cavity through the drainage hole and finally flows into the color developing window through the communicating cavity, and the automatic dripping of the color developing liquid is achieved.

Preferably, as an improvement, the driving mechanism includes a pneumatic tube, a first check valve, a second check valve and a third check valve, the pneumatic tube is communicated with the mixing chamber, the first check valve is located between the buffer tank and the mixing chamber, the second check valve is located between the sample tank and the mixing chamber, and the third check valve is located between the mixing chamber and the fixing groove.

In this scheme, utilize the atmospheric pressure pipe can increase or reduce the atmospheric pressure in the hybrid chamber, the third check valve only enables liquid flow direction fixed slot in the hybrid chamber simultaneously, and first check valve only enables the buffer solution flow direction hybrid chamber in the buffer tank, and the second check valve only enables the sample flow direction hybrid chamber in the sample cell. When the sample and the buffer solution need to be driven into the mixing cavity, the air pressure pipe exhausts air to reduce the air pressure in the mixing cavity, so that the buffer solution in the buffer solution groove and the sample in the sample groove are sucked into the mixing cavity; after buffer solution and sample mix in the hybrid chamber and form mixed liquid, utilize the atmospheric pressure pipe to make the pressure increase in the hybrid chamber to in the mixed liquid drive in the hybrid chamber to the fixed slot, whole actuating mechanism simple structure just can conveniently realize the flow direction control to various liquid.

Preferably, as an improvement, the puncturing mechanism comprises a fixed rod, a movable rod and a puncturing piece fixedly connected to the movable rod, the fixed rod is fixedly connected to the inside of the buffer liquid tank, the movable rod is slidably connected to the fixed rod, and an elastic piece is arranged between the movable rod and the fixed rod; air holes penetrating through the movable rod are formed in the movable rod along the axial direction of the movable rod, and communicating holes communicated with the air holes are formed in the side wall of the buffer liquid tank.

In the scheme, when the buffer liquid bag is placed in the buffer liquid groove, the buffer liquid bag is contacted with the puncturing piece, and when the immunity detection is required, the buffer liquid bag is pushed to enable the puncturing piece to puncture the buffer liquid bag, so that the buffer liquid in the buffer liquid bag flows into the buffer liquid groove; simultaneously, the dead lever and the movable rod of mutual swing joint have been set up in this scheme, after utilizing the piercing member to pierce the buffer package, continue to promote buffer package extrusion movable rod, the elastic component can play the effect of buffering, and simultaneously, because the buffer package constantly extrudees the movable rod, make the buffer package constantly reduce with the volume that the buffer tank encloses, atmospheric pressure increase in the buffer tank, thereby make the gas in the buffer tank enter into the inside of buffer package through intercommunicating pore and bleeder vent, make the inside atmospheric pressure increase of buffer package, thereby make the buffer solution in the buffer tank flow to the buffer tank more fast, realize the quick acquisition of buffer solution.

Preferably, as an improvement, a first quantitative control unit is arranged between the buffer liquid tank and the mixing cavity, and a second quantitative control unit is arranged between the sample tank and the mixing cavity.

In this scheme, utilize first quantitative the control unit to carry out capacity control to the buffer solution that gets into the hybrid chamber in the buffer cistern, utilize the second quantitative the control unit to carry out accurate control to the sample capacity that gets into the hybrid chamber in the sample cell simultaneously to the dilution ratio of accurate control sample and buffer further promotes the precision that nanometer enzyme chromatography detected.

Preferably, as an improvement, be equipped with the waste liquid groove on the card box body, first quantitative the control unit including offering the first overflow hole on the buffer tank lateral wall, second quantitative the control unit including offering the second overflow hole on the sample groove lateral wall, first overflow hole and second overflow hole all communicate with the waste liquid groove.

In this scheme, utilize the overflow hole with in buffer tank or the sample groove unnecessary liquid drainage to the waste liquid groove, not only can the volume of liquid in accurate control buffer tank and the sample groove, can also concentrate the collection in discharging unnecessary liquid to the waste liquid groove automatically simultaneously, avoid buffer solution or sample pollution reagent card box.

Preferably, as a refinement, the puncturing sleeve is screwed to the storage bottle.

In the scheme, the puncture sleeve is in threaded connection with the storage bottle, so that the connection between the puncture sleeve and the storage bottle is stable, meanwhile, when the liquid A and the liquid B are required to be mixed into the color developing liquid and the color developing liquid is conveyed to the drainage hole, the first sharp prick on the puncture sleeve can puncture the first sealing element only by rotating the puncture sleeve, and the puncture sleeve is continuously rotated, so that the second sharp prick punctures the second sealing element, and the operation is very simple; more importantly, when the puncture sleeve is rotated, the puncture sleeve can drive the first sharp thorn and the second sharp thorn to rotate simultaneously, and the first sharp thorn and the second sharp thorn play a role in stirring after mixing the liquid A and the liquid B, so that the liquid A and the liquid B can be mixed more quickly to form the color developing liquid.

Preferably, as a modification, the first sealing member and the second sealing member each include an aluminum film.

In this scheme, utilize the aluminium membrane as the sealing member, the aluminium membrane does not react with the liquid of preparing, and aluminium membrane processing is simple simultaneously, and the leakproofness is good.

Preferably, as an improvement, an elastic reset piece is arranged between the buffer liquid bag and the buffer liquid groove

In the scheme, the elastic reset piece is arranged between the buffer liquid bag and the buffer liquid groove, when the buffer liquid bag is punctured by the puncture piece and buffer liquid in the buffer liquid bag enters the buffer liquid groove, and external force borne by the buffer liquid bag disappears, the buffer liquid bag is far away from the puncture piece under the action of the elastic force of the elastic reset piece, so that the puncture piece moves out of the buffer liquid bag, when the puncture piece moves out of the buffer liquid bag, the puncture piece pulls the puncture surface of the buffer liquid bag outwards, so that the punctured surface forms a curved surface structure which is sunken towards the outside of the buffer liquid bag, all buffer liquid in the buffer liquid bag can flow into the buffer liquid groove, partial buffer liquid is prevented from remaining in the buffer liquid bag, the waste of the buffer liquid is avoided, and the operation is complicated when the buffer liquid bag garbage is subsequently treated due to partial buffer liquid remaining.

Drawings

FIG. 1 is a schematic diagram of a nanoenzyme chromatography reagent cartridge with a buffer bag and a first spring hidden therein according to an embodiment of the present invention.

Fig. 2 is a cross-sectional view taken along a-a in fig. 1.

Fig. 3 is a partially enlarged view of a portion a in fig. 2.

Fig. 4 is a partially enlarged view of fig. 2 at B.

Fig. 5 is a cross-sectional view taken along line B-B of fig. 1.

Fig. 6 is a partial enlarged view of the same point B in fig. 2 in the second embodiment of the present invention.

Detailed Description

The following is further detailed by way of specific embodiments:

reference numerals in the drawings of the specification include: the cartridge comprises a cartridge body 1, a fixed groove 2, a chromatography test paper 3, a buffer liquid tank 4, a sample tank 5, a mixing chamber 6, a buffer liquid bag 7, a pneumatic tube 8, a first one-way valve 9, a second one-way valve 10, a third one-way valve 11, a waste liquid tank 12, a first overflow hole 13, a first pipeline 14, a second overflow hole 15, a second pipeline 16, a magnetic bead 17, a fixed rod 18, a movable rod 19, a puncturing piece 20, a first spring 21, a second spring 22, a vent hole 23, a communication hole 24, a connecting part 25, a communication chamber 26, a storage bottle 27, a puncturing sleeve 28, an A liquid storage chamber 29, a B liquid storage chamber 30, a communication hole 31, a first aluminum film 32, a first sharp puncture 33, a drainage hole 34, a second aluminum film 35 and a stirring sheet 36.

Example one

The first embodiment is substantially as shown in figures 1, 2, 3 and 4 of the accompanying drawings: a nano enzyme chromatography reagent card box comprises a card box body 1 and a fixing groove 2 which is arranged in the card box body 1 and is close to the top, wherein chromatography test paper 3 for nano enzyme chromatography detection is clamped in the fixing groove 2; meanwhile, a buffer liquid groove 4, a sample groove 5 and a mixing cavity 6 are arranged on the card box body 1 close to the left side, communicating pipelines are arranged between the mixing cavity 6 and the buffer liquid groove 4 as well as between the sample groove 5 and the fixing groove 2, a buffer liquid bag 7 is detachably connected in the buffer liquid groove 4, the whole buffer liquid bag 7 is cylindrical, the side wall of the buffer liquid bag 7 is attached to the buffer liquid groove 4, a buffer liquid is filled in the buffer liquid bag 7, and the bottom of the buffer liquid bag 7 seals the buffer liquid in the buffer liquid bag 7 through an adhesive aluminum film; in this embodiment, a driving mechanism for driving the mixing chamber 6 to directionally flow between the buffer solution tank 4, the sample tank 5 and the fixing tank 2 is provided in the cartridge body 1.

Referring to fig. 2 and 3, the driving mechanism includes a pneumatic tube 8, a first check valve 9, a second check valve 10, and a third check valve 11, a left port of the pneumatic tube 8 is disposed on a left sidewall of the cartridge body 1, a right port of the pneumatic tube 8 is disposed on a right sidewall of the mixing chamber 6, and the pneumatic tube 8 can be used to increase or decrease the air pressure in the mixing chamber 6 by externally connecting gas processing elements such as an air pump to the pneumatic tube 8. The first one-way valve 9 is arranged on a communication pipeline between the buffer liquid tank 4 and the mixing cavity 6, so that the liquid in the buffer liquid tank 4 can only flow from the buffer liquid tank 4 to the mixing cavity 6; the second one-way valve 10 is arranged on a communication pipeline between the mixing cavity 6 and the sample groove 5, so that the liquid in the sample groove 5 can only flow from the sample groove 5 to the mixing cavity 6; the third one-way valve 11 is arranged on a communication pipeline between the mixing chamber 6 and the fixing groove 2, so that the liquid in the mixing chamber 6 can only flow to the fixing groove 2 from the mixing chamber 6.

Referring to fig. 3 and 5, a waste liquid tank 12 is opened near the bottom of the left side in the cartridge body 1, a first quantitative control unit is provided between the buffer liquid tank 4 and the mixing chamber 6, and a second quantitative control unit is provided between the sample tank 5 and the mixing chamber 6. In this embodiment, the first quantitative control unit includes a first overflow hole 13 opened on the side wall of the buffer liquid tank 4, and a first pipeline 14 is communicated between the first overflow hole 13 and the waste liquid tank 12; the second quantitative control unit comprises a second overflow hole 15 arranged on the side wall of the sample tank 5, and a second pipeline 16 is communicated between the second overflow hole 15 and the waste liquid tank 12.

As shown in fig. 3, a mixing mechanism is disposed in the mixing chamber 6, in this embodiment, the mixing mechanism includes a magnetic bead 17 movably connected in the mixing chamber 6, in order to enable the magnetic bead 17 to have a better mixing effect, a plurality of limiting rods are bonded in the mixing chamber 6 in this embodiment, a space for placing the magnetic bead 17 is enclosed by four adjacent limiting rods, and under the action of a magnetic field whose external direction changes continuously, the magnetic bead 17 can move back and forth in the mixing chamber 6 to mix liquid in the mixing chamber 6.

Referring to fig. 2 and 3, a puncturing mechanism for puncturing the aluminum film at the bottom of the buffer liquid bag 7 is arranged in the buffer liquid tank 4, in this embodiment, the puncturing mechanism includes a fixed rod 18, a movable rod 19 and a puncturing piece 20 integrally formed at the top end of the movable rod 19, the top of the puncturing piece 20 is in a sharp angle shape, and the top of the puncturing piece 20 is in contact with the aluminum film at the bottom of the buffer liquid bag 7; meanwhile, an elastic resetting piece is arranged between the buffer liquid bag 7 and the buffer liquid groove 4, the elastic resetting piece comprises a first spring 21 sleeved on the buffer liquid bag 7, and the bottom of the first spring 21 is in contact with the bottom wall of the buffer liquid groove 4.

The bottom of the fixed rod 18 is bonded on the bottom wall of the buffer liquid tank 4, the inside of the fixed rod 18 is hollow, the part of the bottom of the movable rod 19 is inserted into the fixed rod 18, an elastic part is arranged between the bottom of the movable rod 19 and the fixed rod 18, and the elastic part comprises a second spring 22 placed in the hollow part of the inside of the fixed rod 18; simultaneously, open along its self axial on the movable rod 19 has the bleeder vent 23 that runs through movable rod 19, and integrated into one piece has a plurality of archs that set up along the movable rod 19 axial on the outer wall of movable rod 19, has the clearance between the adjacent arch, conveniently punctures piece 20 and inserts buffer liquid package 7 back, and the buffer liquid in the buffer liquid package 7 flows out outside buffer liquid package 7 along the clearance between the adjacent arch fast. In this embodiment, the cartridge body 1 is further provided with a communication hole 24, one end of the communication hole 24 communicates with the buffer tank 4, and the other end of the communication hole 24 communicates with the inside of the fixing lever 18 in a hollow manner.

As shown in figure 1, a storage mixing mechanism used for respectively storing the liquid A and the liquid B and preparing the color developing liquid is detachably connected to the cartridge body 1, and a drainage mechanism used for draining the color developing liquid in the storage mechanism into a color developing window is arranged on the cartridge body 1. Combine fig. 2 and fig. 4, drainage mechanism includes integrated into one piece and is close to the connecting portion 25 of right-hand member in card box body 1 top, and it has the intercommunication chamber 26 that communicates each other with the color development window on the card box body 1 to open on the connecting portion 25.

As shown in fig. 4, the storage and mixing mechanism comprises a storage bottle 27 and a puncturing sleeve 28 screwed on the top of the storage bottle 27, the bottom end of the storage bottle 27 is screwed and fixed on the cartridge body 1, an a liquid storage chamber 29 and a B liquid storage chamber 29 for separately storing a liquid a and a liquid B are arranged in the storage bottle 27, in this embodiment, the B liquid storage chamber 29 and the a liquid storage chamber 29 are coaxially arranged, the B liquid storage chamber 29 is positioned in the a liquid storage chamber 29, a communication hole 24 communicated with the a liquid storage chamber 29 is formed at the bottom of the B liquid storage chamber 29, a first sealing member is arranged on the communication hole 24, and the first sealing member comprises a first aluminum film 32 adhered on the communication hole 24; the first sharp prick 33 for pricking the first aluminum film 32 is integrally formed on the pricking sleeve 28, the bottom end of the storage bottle 27 is provided with a drainage hole 34 communicated with the liquid A storage cavity 29, the bottom end of the drainage hole 34 is communicated with the communicating cavity 26, the bottom end of the storage bottle 27 is fixedly connected with a second sealing element for sealing the drainage hole 34, the second sealing element comprises a second aluminum film 35 adhered in the drainage hole 34, and the pricking sleeve 28 is fixedly connected with a second sharp prick for pricking the second sealing element.

The specific implementation process is as follows:

before carrying out the nano enzyme chromatography detection, the buffer liquid bag 7 is placed in the buffer liquid tank 4, the top end of the first spring 21 is contacted with the bottom surface of the buffer liquid bag 7, the first spring 21 plays a role in supporting and stabilizing the buffer liquid bag 7, and at the moment, the puncturing piece 20 is only contacted with the bottom of the buffer liquid bag 7 but cannot puncture the buffer liquid bag 7; meanwhile, the storage bottle 27 is fixedly connected to the connecting part 25 in a threaded mode, the bottom end of the first spike 33 is located above the first aluminum film 32, the liquid A is independently stored in the liquid A storage cavity 29, and the liquid B is independently stored in the liquid B storage cavity 29.

When detection is needed, a sample to be detected is added into the sample tank 5, and when the liquid level of the sample in the sample tank 5 is higher than the second overflow hole 15, the redundant sample flows into the waste liquid tank 12 through the second overflow hole 15 and the second pipeline 16, so that the volume of the sample in the sample tank 5 is a fixed value; meanwhile, the buffer liquid bag 7 is pushed by external force to move downwards as shown in fig. 3, the puncturing piece 20 punctures the aluminum film at the bottom of the buffer liquid bag 7, so that the buffer liquid in the buffer liquid bag 7 flows into the buffer liquid tank 4, when the liquid level of the buffer liquid in the buffer liquid tank 4 is flush with the first overflow hole 13, the redundant buffer liquid automatically flows into the waste liquid tank 12 through the first overflow hole 13 and the first pipeline 14, and the capacity of the buffer liquid in the buffer liquid tank 4 is constant.

In this embodiment, when utilizing external force to promote the buffer package 7 downwards, after piercing member 20 pierced the aluminium membrane on the buffer package 7, piercing member 20 inserted the inside of buffer package 7, continue to promote buffer package 7 downwards, make the internal gas pressure in the space of enclosing between buffer package 7 and the buffer tank 4 bottom wall increase, the gas in buffer tank 4 passes through intercommunicating pore 24, the inside of dead lever 18 is hollow and bleeder vent 23 and enters into the inside of buffer package 7, make the increase of pressure in the buffer package 7, thereby make the buffer solution in the buffer package 7 flow into in the buffer tank 4 more fast, thereby promote the efficiency that detects.

When the buffer solution in the buffer solution bag 7 has substantially flowed into the buffer solution tank 4, the external force on the buffer solution bag 7 is canceled, the buffer solution bag 7 is returned upward by the elastic force of the first spring 21, so that the puncturing member 20 is withdrawn out of the buffer liquid packet 7, and when the puncturing member 20 is withdrawn out of the buffer liquid packet 7, the puncture member 20 can be pulled downwards to puncture the puncture hole of the aluminum film, so that the position of the puncture hole on the aluminum film is lower than the bottom of the buffer liquid bag 7, namely, the punctured surface of the aluminum film forms a curved surface structure which is concave towards the lower part of the buffer liquid bag 7, so that all buffer liquid in the buffer liquid bag 7 can smoothly flow into the buffer liquid groove 4, partial buffer liquid is prevented from remaining in the buffer liquid bag 7, thereby causing the waste of the buffer solution and preventing the complex operation of the subsequent treatment of the garbage in the buffer solution bag 7 caused by the residual of part of the buffer solution.

Then, the air pressure in the mixing chamber 6 is reduced by using the air pressure pipe 8 for air suction, at the moment, the first one-way valve 9 and the second one-way valve 10 are opened (when the first one-way valve 9 is opened, the second one-way valve 10 is opened, and the third one-way valve 11 is in a cut-off state), so that the sample in the sample tank 5 and the buffer solution in the buffer solution tank 4 are sucked into the mixing chamber 6, and after the sample and the buffer solution are sucked into the mixing chamber 6, the magnetic beads 17 are moved back and forth by using an external constantly-changing magnetic field to uniformly mix the sample and the buffer solution.

The sample and the buffer solution are mixed to obtain a mixed solution, then the gas is filled into the mixing cavity 6 through the gas pressure pipe 8, so that the pressure in the mixing cavity 6 is increased, at the moment, the third one-way valve 11 is opened, the first one-way valve 9 and the second one-way valve 10 are both in a cut-off state, the mixed solution in the mixing cavity 6 is pressed to the fixing groove 2, the mixed solution automatically contacts with the chromatographic test paper 3 after entering the fixing groove 2, and the mixed solution automatically moves to a reaction position on the chromatographic test paper 3 along the chromatographic test paper 3 to react under the capillary action.

After the mixed solution reacts with the chromatographic test paper 3 for a period of time, the puncture sleeve 28 is rotated by an external force, so that the puncture sleeve 28 rotates relative to the storage bottle 27 and continuously moves towards the direction close to the storage bottle 27, the first aluminum film 32 is firstly punctured by the first sharp point 33 in the puncture sleeve 28, so that the liquid B in the liquid B storage chamber 29 automatically flows into the liquid A storage chamber 29 through the communication hole 24 to be mixed with the liquid A, the liquid B and the liquid A are mixed to form a developing solution, the puncturing sleeve 28 is continuously rotated by external force in the same direction, so that the first spike 33 continues to move relative to the storage bottle 27 to pierce the second aluminum membrane 35, so that the mixed color-developing solution in the solution A storage chamber 29 automatically flows into the communication chamber 26 through the drainage hole 34, and finally, the color-developing solution automatically flows from the communication chamber 26 to the color-developing window, and the color-developing solution contacts with the chromatographic test paper 3 to develop the color of the chromatographic test paper 3, thereby obtaining the detection result.

In this embodiment, the external force for pushing the buffer liquid bag 7, the air suction or intake in the pneumatic tube 8, and the external force for rotating the puncture sleeve 28 can be achieved by manual control, and can also be achieved by control of other power mechanisms and control units, so that the whole nano enzyme chromatography detection is fully automated.

Example two

The difference between the second embodiment and the first embodiment is that: as shown in fig. 6, in this embodiment, a plurality of stirring pieces 36 are integrally formed on the side wall of the first spike 33, the stirring pieces 36 are axially disposed along the first spike 33, the bottom end of each stirring piece 36 is flush with the bottom end of the first spike 33, and a sharp corner is inverted at the bottom end of each stirring piece 36, when the first aluminum film 32 and the second aluminum film 35 are punctured by the first spike 33, the bottom end of each stirring piece 36 can also puncture the first aluminum film 32 and the second aluminum film 35, so that the first aluminum film 32 and the second aluminum film 35 can be quickly punctured and damaged, the liquid a and the liquid B can be quickly mixed to form the color developing solution, and the color developing solution can quickly enter the communicating cavity 26 from the liquid a storage cavity 29, so that the color developing solution quickly contacts with the chromatographic test paper 3 to complete the color developing reaction; meanwhile, the puncture sleeve 28 can drive the stirring sheet 36 to rotate when rotating, so that the stirring sheet 36 can stir the liquid, when the liquid A is mixed with the liquid B, the liquid A and the liquid B can be quickly mixed with the developing solution by stirring the stirring sheet 36, and the efficiency of the nano enzyme chromatography detection is further improved.

The foregoing is merely an example of the present invention and common general knowledge in the art of designing and/or characterizing particular aspects and/or features is not described in any greater detail herein. It should be noted that, for those skilled in the art, without departing from the technical solution of the present invention, several variations and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims (10)

1. The utility model provides a nanometer enzyme chromatography reagent card box, includes the card box body and sets up in this internal fixed slot of card box, fixedly connected with chromatography test paper in the fixed slot, its characterized in that: the card box body is provided with a buffer liquid tank, a sample tank and a mixing cavity, a mixing mechanism is arranged in the mixing cavity, and the mixing cavity is simultaneously communicated with the buffer liquid tank, the sample tank and the fixing groove; the card box comprises a card box body and a buffer liquid groove, wherein the inside of the buffer liquid groove is detachably connected with a buffer liquid bag, a puncturing mechanism for puncturing the buffer liquid bag is arranged in the buffer liquid groove, and a driving mechanism for driving the mixing cavity to directionally flow between the buffer liquid groove and the card box body as well as between a sample groove and a fixed groove is arranged in the card box body; the card box body is detachably connected with a storage mixing mechanism used for storing the liquid A and the liquid B and preparing the color developing liquid, and the card box body is provided with a drainage mechanism used for draining the color developing liquid in the storage mechanism into the color developing window.

2. The nanoenzyme chromatography reagent cartridge of claim 1, wherein: the storage mixing mechanism comprises a storage bottle and a puncture sleeve movably connected onto the storage bottle, the storage bottle is detachably connected onto the card box body, an A liquid storage cavity and a B liquid storage cavity which are used for separately storing A liquid and B liquid are arranged in the storage bottle, a communicating hole communicated with the A liquid storage cavity is formed in the B liquid storage cavity, a first sealing element is arranged on the communicating hole, and a first sharp puncture which is used for puncturing the first sealing element is fixedly connected onto the puncture sleeve.

3. The nanoenzyme chromatography reagent cartridge of claim 2, wherein: drainage mechanism includes the connecting portion of fixed connection on the card box body, it has the intercommunication chamber with the color development window intercommunication to open on the connecting portion, and it has the drainage hole with connecting portion complex to open on the A liquid storage chamber, fixedly connected with is used for the second sealing member in sealed drainage hole on the storage bottle, punctures and sheathes the second point thorn that fixedly connected with is used for punctureing the second sealing member.

4. The nanoenzyme chromatography reagent cartridge of claim 3, wherein: the driving mechanism comprises an air pressure pipe, a first one-way valve, a second one-way valve and a third one-way valve, the air pressure pipe is communicated with the mixing cavity, the first one-way valve is positioned between the buffer liquid groove and the mixing cavity, the second one-way valve is positioned between the sample groove and the mixing cavity, and the third one-way valve is positioned between the mixing cavity and the fixing groove.

5. The nanoenzyme chromatography reagent cartridge of claim 4, wherein: the puncturing mechanism comprises a fixed rod, a movable rod and a puncturing piece fixedly connected to the movable rod, the fixed rod is fixedly connected to the inside of the buffer liquid tank, the movable rod is connected to the fixed rod in a sliding mode, and an elastic piece is arranged between the movable rod and the fixed rod; air holes penetrating through the movable rod are formed in the movable rod along the axial direction of the movable rod, and communicating holes communicated with the air holes are formed in the side wall of the buffer liquid tank.

6. The nanoenzyme chromatography reagent cartridge of claim 5, wherein: and a first quantitative control unit is arranged between the buffer liquid tank and the mixing cavity, and a second quantitative control unit is arranged between the sample tank and the mixing cavity.

7. The nanoenzyme chromatography reagent cartridge of claim 6, wherein: the card box body is provided with a waste liquid tank, the first quantitative control unit comprises a first overflow hole arranged on the side wall of the buffer liquid tank, the second quantitative control unit comprises a second overflow hole arranged on the side wall of the sample tank, and the first overflow hole and the second overflow hole are communicated with the waste liquid tank.

8. The nanoenzyme chromatography reagent cartridge of any one of claims 3 to 7, wherein: the puncture sleeve is in threaded connection with the storage bottle.

9. The nanoenzyme chromatography reagent cartridge of claim 8, wherein: the first and second sealing members each include an aluminum film.

10. The nanoenzyme chromatography reagent cartridge of claim 9, wherein: and an elastic reset piece is arranged between the buffer liquid bag and the buffer liquid groove.

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