CN214310531U - Kit - Google Patents

Abstract

The utility model relates to the technical field of immunodetection equipment, and discloses a kit, which comprises a card box body and a fixed groove arranged in the card box body, wherein chromatography test paper is fixedly connected in the fixed groove, a buffer liquid groove, a sample groove and a mixing cavity are arranged on the card box body, a mixing mechanism is arranged in the mixing cavity, and the mixing cavity is simultaneously communicated with the buffer liquid groove, the sample groove and the fixed groove; be equipped with the extraction mechanism that is arranged in the sample groove and is arranged in extracting the sample to the sample groove among the vacuum test tube, this internal actuating mechanism that is used for driving between mixing chamber and buffer tank, sample groove and the fixed slot directional flow that is equipped with of card box. In this application only need insert the sample groove with the vacuum test tube when immunodetection, can accomplish the extraction of sample automatically, promote the precision that detects, solved among the prior art and utilized reagent card box to carry out the problem that the operation is complicated and the detection precision is low when immunochromatography detects.

Description

Kit

Technical Field

The utility model relates to an immunoassay equipment technical field, concretely relates to kit.

Background

In the process of immunochromatography detection and analysis, detection is mainly performed on a reagent card box, a chromatography test paper is fixed in the existing reagent card box, a sample hole and a color development window are formed in the top of the reagent card box, when immunoassay is performed, a sample and a buffer solution need to be diluted according to a certain proportion, then the diluted sample is dripped into a sample groove in the reagent card box, the diluted sample is made to be in contact with the chromatography test paper in the reagent card box, and after the chromatography test paper reacts with the sample for a period of time, a detection result is measured through the color development window.

In current reagent card box, when carrying out immunochromatography and detecting, need the manual dropwise add buffer solution in to the sample and dilute the sample, need strictly control the dilution ratio of sample when detecting to promote the precision that detects. At present, a sample and a buffer solution are extracted manually, and then the buffer solution and the sample are mixed, so that the operation is complex and the extraction amount is not easy to accurately control when the sample and the buffer solution are extracted manually, and a certain deviation exists in a detection result; simultaneously, mix the back with sample and buffer solution manual among the prior art, still need manual sample dropwise add after mixing to the chromatography test paper department of reagent card box, the dropwise add in-process sample appears spilling hourglass easily, causes the influence to the precision of testing result.

SUMMERY OF THE UTILITY MODEL

The utility model provides a kit to solve and utilize reagent card box to carry out immunochromatography among the prior art and examine the problem that the operation is complicated and the detection precision is low.

In order to achieve the above purpose, the utility model adopts the following technical scheme: a kit comprises a card box body and a fixed groove arranged in the card box body, wherein chromatography test paper is fixedly connected in the fixed groove, a buffer liquid groove, a sample groove and a mixing cavity are arranged on the card box body, a mixing mechanism is arranged in the mixing cavity, and the mixing cavity is simultaneously communicated with the buffer liquid groove, the sample groove and the fixed groove; be equipped with the extraction mechanism that is arranged in the sample groove and is arranged in extracting the sample to the sample groove among the vacuum test tube, this internal actuating mechanism that is used for driving between mixing chamber and buffer tank, sample groove and the fixed slot directional flow that is equipped with of card box.

The principle of the scheme is as follows: in the application, when immunoassay needs to be performed, a vacuum blood collection tube is inversely inserted into a sample tank, a diluent is dripped into a buffer liquid tank, a sample in the vacuum blood collection tube is extracted into the sample tank by using an extraction mechanism, a buffer liquid in the buffer liquid tank and a sample in the sample tank are driven by using a driving mechanism to enter a mixing cavity, and the buffer liquid and the sample in the mixing cavity are uniformly mixed by the mixing mechanism, so that a sample to be detected (hereinafter referred to as a mixed liquid) after the sample and the buffer liquid are mixed is obtained; and then, continuously driving the mixed solution in the mixing cavity to the fixed groove by using the driving mechanism, so that the mixed solution is contacted with the chromatography test paper, and thus the sample adding of the mixed solution is completed.

The beneficial effect of this scheme is:

1. reducing the operation steps: compare and need artifical extraction sample and buffer solution among the prior art to need artifical mixed sample and buffer solution, by the manual work with mixed liquid dropwise add to the chromatography test paper department in the card box body at last, whole process operation is complicated. In this application, when detecting, only need insert the sample cell with the vacuum test tube in, then add buffer solution to the buffer solution cistern, can accomplish the extraction and the mixture of buffer solution and sample automatically to the automatic chromatography test paper department that adds of mixed liquid after will mixing, the manual operation step reduces, the simple and convenient more of testing process.

2. The accuracy of immunodetection is favorably improved: in the application, the sample and the buffer solution are automatically extracted by the driving mechanism, so that the condition that the buffer solution is leaked to be extracted and the sample is directly added to the chromatographic test paper for detection can be avoided; simultaneously, need not artifically in this application by the vacuum test tube in the sample of extraction, can reduce the contaminated probability of sample to make and detect and receive the interference factor and reduce, the degree of accuracy of detecting is higher.

Preferably, as an improvement, the sample slot is positioned at the top of the card box body, the extraction mechanism comprises a long extraction needle and a short extraction needle which are fixedly connected in the sample slot, the card box body is provided with an air hole, and the long extraction needle is communicated with the air hole; the short drawing needle is communicated with the mixing cavity, and the distance between the long drawing needle and the top of the card box body is smaller than the distance between the short drawing needle and the top of the card box body.

In this scheme, after inserting the sample groove with the vacuum test tube inversion, the sample is located and is close to the bottom of inversion back vacuum test tube, long needle and the short needle of taking out all insert the vacuum test tube this moment, because the distance of long needle and card box body top is less than the distance of short needle and card box body top, consequently, the top of long needle is higher than the liquid level of the intraductal sample of vacuum test, and the top of short needle of taking out is located sample liquid level below, when utilizing actuating mechanism drive sample in the sample groove to get into the hybrid chamber, can directly draw the sample from the intraductal extraction of vacuum test tube by short needle, and simultaneously, outside air gets into in the vacuum test tube through bleeder vent and long needle, avoid drawing the sample because of short needle and cause the inside negative pressure state that forms of vacuum test tube, thereby place short needle and can't easily draw the sample from the intraductal extraction of vacuum test tube.

Preferably, as an improvement, the driving mechanism includes a first pneumatic tube, a first check valve, a second check valve and a third check valve, the first pneumatic tube is communicated with the mixing chamber, the first check valve is located between the buffer liquid tank and the mixing chamber, the second check valve is located between the short draw needle and the mixing chamber, and the third check valve is located between the mixing chamber and the fixing groove.

In this scheme, utilize first atmospheric pressure pipe can increase or reduce the atmospheric pressure in the hybrid chamber, and the third check valve only enables liquid flow direction fixed slot in the hybrid chamber simultaneously, and first check valve only enables the buffer solution flow direction hybrid chamber in the buffer solution cistern, and the second check valve only enables the sample flow direction hybrid chamber in the vacuum test tube. When the sample and the buffer solution need to be driven into the mixing cavity, the first air pressure pipe exhausts air to reduce the air pressure in the mixing cavity, so that the buffer solution in the buffer solution tank and the sample in the vacuum blood collection pipe are pumped into the mixing cavity; after buffer solution and sample mix in the hybrid chamber and form mixed liquid, utilize first atmospheric pressure pipe to make the pressure increase in the hybrid chamber to in the mixed liquid drive to the fixed slot in the hybrid chamber, whole actuating mechanism simple structure just can conveniently realize the flow direction control to various liquid.

Preferably, as an improvement, be equipped with the ration chamber between mixing chamber and the short extraction needle, it has first overflow mouth to open on the ration chamber, and it has the first waste liquid chamber with first overflow mouth intercommunication to open on the card box body, and the intercommunication has the second pneumatic tube on the first waste liquid chamber, is equipped with the fourth check valve between short extraction needle and the ration chamber.

In this scheme, extract the gas in the first waste liquid chamber through the second pneumatic tube, make first waste liquid chamber and ration chamber all be negative pressure state, thereby make the sample in the vacuum test tube taken out to the ration chamber in, when the liquid level of sample is about to surpass first overflow mouth in the ration chamber, unnecessary sample flows to first waste liquid chamber through first overflow mouth is automatic, thereby make the capacity of the sample in the ration chamber fixed, when making buffer solution and sample mix, can make the buffering proportion of sample and buffer solution more accurate, thereby promote detection effect and make the detection more accurate.

Preferably, as an improvement, open in the buffer tank has the second overflow hole, open on the card box body have with the second waste liquid chamber of second overflow hole intercommunication.

In this scheme, when dropwise add buffer solution to the buffer solution tank in, when the liquid level of buffer solution is about to surpass the second overflow hole in the buffer solution tank, unnecessary buffer solution flows into the second waste liquid chamber through the second overflow hole is automatic in the buffer solution tank to make the capacity of buffer solution fixed, when making buffer solution and sample mix, can make the buffering proportion of sample and buffer solution more accurate, thereby promote detection effect and make the detection more accurate.

Preferably, as an improvement, the mixing mechanism includes magnetic beads movably connected in the mixing chamber.

In this scheme, utilize the magnetic bead to mix buffer solution and sample in the mixing chamber, can be so that sample and buffer solution rapid mixing are even, reduce the problem such as bubble in the mixing process simultaneously.

Preferably, as an improvement, a fixing mechanism for fixing the vacuum blood collection tube is arranged in the sample tank.

In this scheme, utilize fixed establishment to fix the vacuum test tube, can avoid among the testing process vacuum test tube to drop out outside the card box body to prevent that vacuum test tube from causing the influence to immunodetection.

Preferably, as an improvement, fixed establishment is equipped with the elastic component including rotating a plurality of fixture blocks of connecting in the sample inslot between fixture block and the card box body, and the polylith fixture block encloses the joint space that is used for the end cover of joint vacuum test tube.

In this scheme, when inserting the vacuum test tube in the sample groove, the end cover of vacuum test tube promotes the fixture block and rotates, breaks away from the back with the fixture block when the end cover of vacuum test tube, and the fixture block resets under the spring action of elastic component to make the fixture block die the end cover card of vacuum test tube in the sample groove, realize the fixed to the vacuum test tube.

Preferably, as an improvement, a filter screen is fixedly connected to the air vent.

In this scheme, through set up the filter screen in venthole department, avoid the foreign matter to block up the bleeder vent, make the detection go on smoothly.

Preferably, as an improvement, a transparent plate for covering the color development window is fixedly connected to the cartridge body.

In this scheme, utilize the transparent plate to cover the development window, make the reaction position of chromatography test paper be in encapsulated situation, reduce the influence of external environment factor on the reaction on the chromatography test paper to make immunodetection's the degree of accuracy higher.

Drawings

Fig. 1 is a schematic view of a kit according to an embodiment of the present invention.

Fig. 2 is a cross-sectional view taken along a-a in fig. 1.

Fig. 3 is a partial enlarged view of a portion a in fig. 2.

Fig. 4 is a cross-sectional view taken along line B-B of fig. 1.

Fig. 5 is an enlarged partial view of the same position as the position a in fig. 2 in the second embodiment of the present invention.

Detailed Description

The following is further detailed by way of specific embodiments:

reference numerals in the drawings of the specification include: the cartridge comprises a cartridge body 1, a color development window 2, chromatography test paper 3, a buffer liquid tank 4, a sample tank 5, a mixing chamber 6, a vacuum blood collection tube 7, a long drawing needle 8, a short drawing needle 9, an air hole 10, a quantitative chamber 11, a first waste liquid chamber 12, a second air pressure tube 13, a fourth one-way valve 14, a first air pressure tube 15, a first one-way valve 16, a second one-way valve 17, a third one-way valve 18, a second overflow hole 19, a magnetic bead 20 and a cartridge 21.

Example one

The first embodiment is substantially as shown in figures 1, 2 and 3: a kit comprises a card box body 1, wherein the top of the card box body 1 is provided with a color development window 2, meanwhile, a fixing groove is arranged in the card box body 1 close to the top, and a chromatography test paper 3 for fluorescence chromatography detection is clamped in the fixing groove; simultaneously, be close to the left side on the card box body 1 and be equipped with buffer liquid groove 4, sample groove 5 and hybrid chamber 6, hybrid chamber 6 and buffer liquid groove 4, all be provided with the intercommunication pipeline between sample groove 5 and the fixed slot, sample groove 5 is located the top of card box body 1 and is close to left side position, can dismantle in the sample groove 5 and be connected with vacuum test tube 7, and be equipped with in the sample groove 5 and be arranged in extracting the mechanism of sample extraction to sample groove 5 in the vacuum test tube 7, in this embodiment, the vertical sliding connection of end cover of vacuum test tube 7 is on the lateral wall of sample groove 5. In this embodiment, a driving mechanism for driving the mixing chamber 6 to directionally flow between the buffer solution tank 4, the sample tank 5 and the fixing tank is provided in the cartridge body 1.

Combine fig. 2 and fig. 3, extraction mechanism includes that integrated into one piece takes out needle 8 and short needle 9 of taking out on 5 diapalls of sample groove, long needle 8 and short needle 9 of taking out are all along vertical setting, and long needle 8 and the equal inside cavity setting of short needle 9 of taking out, the top of long needle 8 of taking out and the top of short needle 9 of taking out all are located sample groove 5, and the length of long needle 8 of taking out is greater than the length of short needle 9 of taking out, it has the bleeder vent 10 with long needle 8 intercommunication to open on the left side wall of card box body 1, make outside air can enter into long needle 8 through bleeder vent 10 in, in order to avoid foreign matter to block up bleeder vent 10, it has the filter screen that covers bleeder vent 10 to bond on the left side wall of card box body 1.

With reference to fig. 1 and 4, a quantitative cavity 11 and a first waste liquid cavity 12 located below the quantitative cavity 11 are formed in the cartridge body 1, and a communication pipeline is arranged between the quantitative cavity 11 and the short extraction needle 9, so that a sample in the vacuum blood collection tube 7 can enter the quantitative cavity 11 through the short extraction needle 9. Meanwhile, the quantitative cavity 11 is provided with a first overflow port, and a communication pipeline is arranged between the first waste liquid cavity 12 and the first overflow port, so that redundant samples in the quantitative cavity 11 can flow into the first waste liquid cavity 12 along the first overflow port. In order to control the flow direction of the sample in the quantitative cavity 11, a second pneumatic tube 13 communicated with the first waste liquid cavity 12 is arranged on the card box body 1, and a fourth one-way valve 14 is arranged between the short draw needle 9 and the quantitative cavity 11.

With reference to fig. 2 and 3, the driving mechanism includes a first pneumatic tube 15, a first check valve 16, a second check valve 17, and a third check valve 18, a left port of the first pneumatic tube 15 is disposed on a left side wall of the cartridge body 1, a right port of the first pneumatic tube 15 is disposed on a right side wall of the mixing chamber 6, and the first pneumatic tube 15 can be used to increase or decrease the pneumatic pressure in the mixing chamber 6 by externally connecting a gas processing element such as an air pump to the first pneumatic tube 15. The first one-way valve 16 is arranged on a communication pipeline between the buffer liquid tank 4 and the mixing cavity 6, so that the liquid in the buffer liquid tank 4 can only flow from the buffer liquid tank 4 to the mixing cavity 6; the second one-way valve 17 is arranged on a communication pipeline between the mixing cavity 6 and the quantitative cavity 11, so that the liquid in the quantitative cavity 11 can only flow to the mixing cavity 6 from the quantitative cavity 11; the third one-way valve 18 is arranged in the communication channel between the mixing chamber 6 and the stationary trough so that the liquid in the mixing chamber 6 can only flow from the mixing chamber 6 to the stationary trough.

In order to reasonably control the volume of the buffer solution in the buffer solution tank 4, in this embodiment, the inner wall of the buffer solution tank 4 is provided with a second overflow hole 19, and the card box body 1 is provided with a second waste solution cavity communicated with the second overflow hole 19, when the buffer solution is added into the buffer solution tank 4, if the liquid level of the buffer solution is about to overflow the second overflow hole 19, the redundant buffer solution in the buffer solution tank 4 can automatically overflow into the second waste solution cavity through the second overflow hole 19.

As shown in fig. 3, a mixing mechanism is arranged in the mixing chamber 6, the mixing mechanism includes a magnetic bead 20 movably connected in the mixing chamber 6, in order to make the magnetic bead 20 have a better mixing effect, a plurality of limiting rods are bonded in the mixing chamber 6 in the embodiment, the adjacent four limiting rods enclose a space for placing the magnetic bead 20, and under the action of a magnetic field whose external direction changes continuously, the magnetic bead 20 can move back and forth in the mixing chamber 6 to mix the liquid in the mixing chamber 6.

The specific implementation process is as follows:

before the immunochromatographic test, the evacuated blood collection tube 7 is inserted upside down into the sample vessel 5 so that the end cap of the evacuated blood collection tube 7 is in contact with the bottom wall of the sample vessel 5, and as shown in fig. 3, the tip of the long draw needle 8 is located above the surface of the sample liquid in the evacuated blood collection tube 7, and the tip of the short draw needle 9 is located below the surface of the sample liquid.

When detection is needed, the buffer solution is added into the sample tank 5, after the liquid level of the buffer solution is higher than the second overflow hole 19, the redundant buffer solution flows into the second waste liquid chamber through the second overflow hole 19, so that the capacity of the buffer solution in the buffer solution tank 4 is a fixed value, then air is pumped by using the first air pressure pipe 15, so that the air pressure in the mixing chamber 6 is reduced and is in a negative pressure state, at the moment, the second one-way valve 17 is cut off, the first one-way valve 16 is opened, and the buffer solution in the buffer solution tank 4 is sucked into the mixing chamber 6.

After the buffer solution enters the mixing cavity 6, the second air pressure tube 13 is used for pumping air, so that the air pressure in the quantitative cavity 11 is reduced to generate adsorption capacity, at the moment, the fourth one-way valve 14 is opened, the second one-way valve 17 is in a cut-off state, so that the sample in the vacuum blood collection tube 7 is pumped into the quantitative cavity 11 by using the short pumping needle 9, and when the liquid level of the sample in the quantitative cavity 11 is higher than the first overflow hole, the redundant sample enters the first waste liquid cavity 12 through the first overflow hole and is collected. When a sufficient and quantitative amount of sample enters the quantitative chamber 11 (i.e. when an excessive amount of sample enters the first waste liquid chamber 12), the second pneumatic tube 13 is used to inflate the quantitative chamber 11, so that the pressure in the quantitative chamber 11 increases, and the fourth check valve 14 is in a blocking state, so that the sample in the quantitative chamber 11 enters the mixing chamber 6 through the second check valve 17.

After the sample and the buffer are sucked into the mixing chamber 6, the sample and the buffer are mixed by the magnetic beads 20 using an external constantly changing magnetic field. The sample and the buffer solution are mixed to obtain a mixed solution, then the first pneumatic tube 15 is used for filling gas into the mixing cavity 6, so that the pressure in the mixing cavity 6 is increased, at the moment, the third one-way valve 18 is opened, the first one-way valve 16 and the second one-way valve 17 are both in a cut-off state, the mixed solution in the mixing cavity 6 is pressed to the fixed groove, the mixed solution automatically contacts with the chromatographic test paper 3 after entering the fixed groove, and under the capillary action, the mixed solution automatically moves to the reaction position on the chromatographic test paper 3 along the chromatographic test paper 3 to react.

Example two

The difference between the second embodiment and the first embodiment is that: in this embodiment, in order to make the vacuum blood collection tube 7 in a stable state during the detection, a fixing mechanism for fixing the vacuum blood collection tube 7 is provided in the sample tank 5, as shown in figure 5, the fixing mechanism includes two blocks 21 rotatably connected in the sample slot 5 by a pin, an elastic member is provided between the blocks 21 and the cartridge body 1, the elastic member includes a torsion spring, the two blocks 21 enclose a clamping space for clamping the end cap of the vacuum blood collection tube 7, when vacuum blood collection tube 7 is inserted into sample groove 5, vacuum blood collection tube 7 first pushes and rotates latch 21 to increase the distance between two latches 21, when the end cover of vacuum test tube 7 moved to the below of two fixture blocks 21, fixture block 21 resets under the effect of torsional spring, and two fixture blocks 21 can be with vacuum test tube 7 block this moment for vacuum test tube 7 is fixed in sample groove 5.

EXAMPLE III

The difference between the third embodiment and the second embodiment is that: in this embodiment, it has the transparent plate that covers color development window 2 to bond on card box body 1, and the transparent plate is the transparence, can not influence the testing result who observes in color development window 2, and the transparent plate can reduce the interference of external environment to 3 reactions of chromatography test paper simultaneously to promote the precision that detects.

The above description is only an example of the present invention, and the detailed technical solutions and/or characteristics known in the solutions are not described too much here. It should be noted that, for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims (10)

1. The utility model provides a kit, includes the card box body and sets up in this internal fixed slot of card box, fixedly connected with chromatography test paper in the fixed slot, its characterized in that: the card box body is provided with a buffer liquid tank, a sample tank and a mixing cavity, a mixing mechanism is arranged in the mixing cavity, and the mixing cavity is simultaneously communicated with the buffer liquid tank, the sample tank and the fixing groove; the box body is internally provided with a driving mechanism for driving the mixing cavity to directionally flow between the buffer liquid tank, the sample tank and the fixing tank.

2. A kit according to claim 1, wherein: the sample groove is positioned at the top of the card box body, the extraction mechanism comprises a long extraction needle and a short extraction needle which are fixedly connected in the sample groove, the card box body is provided with air holes, and the long extraction needle is communicated with the air holes; the short drawing needle is communicated with the mixing cavity, and the distance between the long drawing needle and the top of the card box body is smaller than the distance between the short drawing needle and the top of the card box body.

3. A kit according to claim 2, wherein: the driving mechanism comprises a first pneumatic tube, a first one-way valve, a second one-way valve and a third one-way valve, the first pneumatic tube is communicated with the mixing cavity, the first one-way valve is located between the buffer liquid groove and the mixing cavity, the second one-way valve is located between the short drawing needle and the mixing cavity, and the third one-way valve is located between the mixing cavity and the fixing groove.

4. A kit according to claim 3, wherein: be equipped with quantitative chamber between hybrid chamber and the short needle of taking out, it has first overflow mouth to open on the quantitative chamber, and it has the first waste liquid chamber with first overflow mouth intercommunication to open on the card box body, and the intercommunication has the second pneumatic tube on the first waste liquid chamber, is equipped with the fourth check valve between short needle of taking out and the quantitative chamber.

5. A kit according to claim 4, wherein: the buffer liquid groove is internally provided with a second overflow hole, and the card box body is provided with a second waste liquid cavity communicated with the second overflow hole.

6. A kit according to claim 5, wherein: the mixing mechanism comprises magnetic beads movably connected in the mixing cavity.

7. A kit according to any one of claims 2 to 6, wherein: and a fixing mechanism for fixing the vacuum blood collection tube is arranged in the sample groove.

8. A kit according to claim 7, wherein: fixing mechanism is equipped with the elastic component including rotating a plurality of fixture blocks of connecting in the sample inslot between fixture block and the card box body, and polylith fixture block encloses into the joint space that is used for the end cover of joint vacuum test tube.

9. A kit according to claim 8, wherein: the filter screen is fixedly connected with the air vent.

10. A kit according to claim 9, wherein: and a transparent plate for covering the color development window is fixedly connected to the card box body.

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