CN210972073U - Apoptosis detect reagent box - Google Patents
Apoptosis detect reagent box Download PDFInfo
- Publication number
- CN210972073U CN210972073U CN201921731628.3U CN201921731628U CN210972073U CN 210972073 U CN210972073 U CN 210972073U CN 201921731628 U CN201921731628 U CN 201921731628U CN 210972073 U CN210972073 U CN 210972073U
- Authority
- CN
- China
- Prior art keywords
- test tube
- multiunit
- reagent
- recess
- reagent box
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 40
- 230000006907 apoptotic process Effects 0.000 title claims abstract description 17
- 238000001514 detection method Methods 0.000 claims description 6
- 241000973497 Siphonognathus argyrophanes Species 0.000 claims description 5
- 239000007788 liquid Substances 0.000 abstract description 5
- 238000000151 deposition Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 7
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 2
- 230000007035 DNA breakage Effects 0.000 description 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 108010047956 Nucleosomes Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001640 apoptogenic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 210000001623 nucleosome Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a cell apoptosis detect reagent box, including kit body, reagent lid, still include the test tube, through hinged joint between kit body and the reagent lid, kit body upper surface be array structure seted up the multiunit with test tube assorted recess, multiunit the recess both ends the joint groove, multiunit have been seted up to the kit upper surface the inside buffer layer, multiunit that all is provided with of recess the bottom of the groove all is provided with the sponge layer, the test tube both ends be provided with joint groove assorted joint piece just be provided with the scale on the joint piece, the inboard array structure that becomes of reagent lid is provided with the multiunit sprue. The utility model has the advantages of can prevent that the test tube is broken, the stability of depositing is strong, can guarantee that the liquid in the test tube can not spill because the kit body is invertd and is confused, its mainly used reagent is deposited.
Description
Technical Field
The utility model relates to a technical field of kit, specific apoptosis detect reagent box that says so.
Background
The apoptosis detection experiment is necessary for researching cells, when the cells undergo apoptosis, some DNA endonucleases can be activated, the endonucleases can cut off genome DNA among nucleosomes, electrophoresis detection is carried out according to DNA extracted during apoptosis, the principle that green fluorescent probe fluorescein is added under the catalysis of terminal deoxynucleotidyl transferase to exposed-OH when the genome DNA is broken can be found, the apoptosis is detected by a TUNE L method, and apoptotic cells showing green fluorescence can be detected by a fluorescence microscope or a flow cytometer after the cells or tissues which are fixed and washed are subjected to one-step staining reaction, so that the apoptosis can be detected at the single cell level, and the early apoptosis can be detected due to DNA breakage in the early apoptosis stage, the cells are generally easier to mark during the TUNE L detection, and necrotic cells are not easy to mark.
In the prior art, a reagent pad is arranged in a box body to place a measuring funnel box, a TdT enzyme (test tube), a fluorescent labeling liquid (test tube) and a TdT enzyme diluent (test tube), but in actual use, when the reagent box is carried and taken, due to negligence, the reagent box is easily inverted, so that a plurality of test tubes fall off from the reagent pad and are mixed or even broken, and therefore the improvement is needed.
SUMMERY OF THE UTILITY MODEL
To the above-mentioned weak point that exists among the prior art, the utility model aims at providing a can prevent that the test tube is broken, the stability of depositing is strong, can guarantee that the liquid in the test tube can not spill the kit that obscures because the kit body is invertd.
The utility model discloses a realize that the technical scheme that above-mentioned purpose adopted is: the utility model provides an apoptosis detect reagent box, includes kit body, reagent lid, still includes the test tube, through hinged joint between kit body and the reagent lid, kit body upper surface be array structure seted up the multiunit with test tube assorted recess, multiunit the recess both ends joint groove, multiunit have been seted up to the kit upper surface the inside buffer layer, the multiunit of all being provided with of recess the recess bottom all is provided with the sponge layer, the test tube both ends be provided with joint groove assorted joint piece just be provided with the scale on the joint piece, reagent lid inboard one-tenth array structure is provided with the multiunit sprue.
The reagent box cover is characterized in that a handle is fixedly arranged on the outer side of the reagent box cover, and a rubber layer is arranged on the handle.
The reagent box cover with be provided with the electromagnetic lock between the kit body, the kit body outside is provided with the electromagnetic lock switch.
The quantity of sprue with the quantity of recess is unanimous, just the size and the test tube mouth phase-match of sprue, the sprue is detachable structure.
The utility model has the advantages that: the test tubes in the kit body can be prevented from being broken through the shock-absorbing layer in the groove and the sponge layer at the bottom of the groove; the clamping grooves at the two ends of the groove are matched with the clamping blocks at the two ends of the test tube for use, so that the storage stability can be improved during storage, and the scales on the clamping blocks can play an auxiliary role; through the sprue of reagent lid inboard, when the transport with the kit body of taking, close the reagent lid, the sprue card seals the test tube in advancing the test tube mouth, can guarantee that the liquid in the test tube can not spill because of the inversion of kit body and mix up.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic top view of the kit body according to the present invention;
FIG. 3 is a schematic view of the structure of the test tube of the present invention;
fig. 4 is a schematic view of the structure of the test tube of the present invention.
In the figure: 1 kit body, 2 reagent lid, 3 test tubes, 4 recesses, 5 joint grooves, 6 buffer layers, 7 sponge layers, 8 joint pieces, 9 sprue, 10 handles, 11 electromagnetic lock switches.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Please refer to fig. 1-4, a kit for detecting apoptosis, including kit body 1, reagent lid 2, still include test tube 3, through hinged joint between kit body 1 and reagent lid 2, kit body 1 upper surface is the array structure and has seted up multiunit and test tube 3 assorted recess 4, joint groove 5 has been seted up to the kit upper surface at multiunit recess 4 both ends, multiunit recess 4 is inside all to be provided with buffer layer 6, multiunit recess 4 bottom all is provided with sponge layer 7, test tube 3 both ends are provided with joint groove 5 assorted joint piece 8 and be provided with the scale on the joint piece 8, reagent lid 2 inboard one-tenth array structure is provided with multiunit sprue 9.
In the utility model, a handle 10 is fixedly arranged at the outer side of the reagent box cover 2, and a rubber layer is arranged on the handle 10;
an electromagnetic lock is arranged between the reagent box cover 2 and the reagent box body 1, and an electromagnetic lock switch 11 is arranged on the outer side of the reagent box body 1;
the quantity of sprue 9 is unanimous with the quantity of recess 4, and sprue 9's size and test tube mouth phase-match, and sprue 9 is detachable structure.
The utility model discloses a theory of operation is: when the kit is used, the test tubes 3 in the kit body 1 can be prevented from being broken through the shock absorption layer 6 in the groove 4 and the sponge layer 7 at the bottom of the groove 4; the clamping grooves 5 at the two ends of the groove 4 are matched with the clamping blocks 8 at the two ends of the test tube 3 for use, so that the storage stability can be improved during storage, and the scales on the clamping blocks 8 can play an auxiliary role; through the sprue 9 of 2 inboards of reagent lid, when carrying and the kit body 1 of taking, close reagent lid 2, the sprue 9 card seals test tube 3 in going into the test tube mouth, can guarantee that the liquid in the test tube 3 can not spill because of inversion of kit body 1 and mix up.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (4)
1. The utility model provides an apoptosis detect reagent box, includes kit body (1), reagent lid (2), its characterized in that: still include test tube (3), through hinged joint between kit body (1) and reagent lid (2), kit body (1) upper surface be array structure seted up the multiunit with test tube (3) assorted recess (4), multiunit recess (4) both ends joint groove (5), multiunit have been seted up to the kit upper surface recess (4) inside shock-absorbing layer (6), the multiunit all be provided with sponge layer (7) recess (4) bottom, test tube (3) both ends be provided with joint groove (5) assorted joint piece (8) just be provided with the scale on joint piece (8), reagent lid (2) inboard one-tenth array structure is provided with multiunit sprue (9).
2. The apoptosis detection kit according to claim 1, wherein: the reagent box cover is characterized in that a handle (10) is fixedly mounted on the outer side of the reagent box cover (2), and a rubber layer is arranged on the handle (10).
3. The apoptosis detection kit according to claim 1, wherein: an electromagnetic lock is arranged between the reagent box cover (2) and the reagent box body (1), and an electromagnetic lock switch (11) is arranged on the outer side of the reagent box body (1).
4. The apoptosis detection kit according to claim 1, wherein: the quantity of sprue (9) with the quantity of recess (4) is unanimous, just the size and the test tube mouth phase-match of sprue (9), sprue (9) are detachable structure.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201921731628.3U CN210972073U (en) | 2019-10-16 | 2019-10-16 | Apoptosis detect reagent box |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201921731628.3U CN210972073U (en) | 2019-10-16 | 2019-10-16 | Apoptosis detect reagent box |
Publications (1)
Publication Number | Publication Date |
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CN210972073U true CN210972073U (en) | 2020-07-10 |
Family
ID=71421936
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201921731628.3U Expired - Fee Related CN210972073U (en) | 2019-10-16 | 2019-10-16 | Apoptosis detect reagent box |
Country Status (1)
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CN (1) | CN210972073U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116297427A (en) * | 2023-03-10 | 2023-06-23 | 多莱泌生物科技(武汉)有限公司 | Exosome concentration detection kit |
-
2019
- 2019-10-16 CN CN201921731628.3U patent/CN210972073U/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116297427A (en) * | 2023-03-10 | 2023-06-23 | 多莱泌生物科技(武汉)有限公司 | Exosome concentration detection kit |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200710 |
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CF01 | Termination of patent right due to non-payment of annual fee |