CN116268009A - Novel biological pesticide and preparation method and application thereof - Google Patents

Novel biological pesticide and preparation method and application thereof Download PDF

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Publication number
CN116268009A
CN116268009A CN202310052556.4A CN202310052556A CN116268009A CN 116268009 A CN116268009 A CN 116268009A CN 202310052556 A CN202310052556 A CN 202310052556A CN 116268009 A CN116268009 A CN 116268009A
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parts
extract
novel
essential oil
plant
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阳忠禄
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Chongqing Nuoyi Pesticide Co ltd
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Chongqing Nuoyi Pesticide Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
    • A01N33/18—Nitro compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/58—1,2-Diazines; Hydrogenated 1,2-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
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Abstract

The invention provides a novel biological pesticide which is characterized by comprising the following raw materials in parts by weight: 10-20 parts of antibacterial mixed solution, 20-30 parts of plant growth regulator, 12-18 parts of stramonium extract, 8-12 parts of rosemary extract, 6-10 parts of lemon thyme extract, 6-10 parts of negundo chastetree extract, 10-15 parts of pyrethrum, 10-20 parts of perilla essential oil, 10-15 parts of wintergreen plant essential oil, 5-10 parts of methyl caffeate, 5-10 parts of 6, 7-dihydroxycoumarin and 10-15 parts of surfactant. The novel biopesticide provided by the invention is environment-friendly, low in residue, good in stability and good in pest control effect, and has good application prospects in plant cultivation and pest control.

Description

Novel biological pesticide and preparation method and application thereof
Technical Field
The invention relates to the technical field of pesticides, in particular to a novel biological pesticide and a preparation method and application thereof.
Background
Agriculture is the basis for human survival, is the basis for national economy, and with the continuous increase of population, more grain output is required to meet the population growing demands. Grain production depends on cultivated land resources, the cultivated land resources are limited, according to the statistics of grain and agricultural organization (FAO) of united countries, the cultivated land area of the whole world is about 14 hundred million hectares in 2000, the cultivated land area of 2013 is increased to 14.08 hundred million public, the cultivated land area is hardly increased in more than ten years, and the growing speed is far lower than the growing speed of grain requirements, so that the growing of the grain requirements is difficult to be completely met by the growing of the cultivated land area, measures are needed to be taken to improve the grain yield of unit cultivated land area by improving the refinement degree of agriculture, such as seed improvement, strengthening crop plant protection measures and the like, and the important roles of biotechnology and agricultural chemistry in production are fully embodied.
The pesticide is used as important production data in agricultural production, and plays an important role in preventing and controlling diseases, insect pests and weeds. The contribution of pesticides to agricultural production is widely accepted, if no pesticides are used, the grain yield is reduced by 30-40%, and the survival and development of human beings are directly threatened. Therefore, the pesticide plays an extremely important role in protecting crops from being damaged by harmful organisms, guaranteeing grain supply and developing national economy. The development of the world pesticides has been generally carried out in three periods, the first period is before the 70 th year of the 19 th century, and natural products such as sulfur and the like are utilized for controlling plant diseases and insect pests; the second period is from the 70 th year of the 19 th century to the 40 th year of the 20 th century, the inorganic pesticides are used in a large amount, the natural products and inorganic compounds are used in a large amount, the third period is after the 40 th year of the 20 th century, the artificially synthesized pesticides are used as organic pesticides, and the artificially synthesized pesticides have the forms of dripping, 2,4-D, six and the like, so that the human beings end the stage of pest control mainly relying on the inorganic pesticides and the natural products. Since then, a large number of pesticides have been synthesized and named chemical pesticides. Early organic synthetic pesticides mainly comprise three main types of organophosphorus pesticides, organochlorine pesticides and carbamic acid cool pesticides, and then bactericides and herbicides are also developed. The traditional pesticide preparation comprises emulsifiable concentrate, wettable powder, granules and powder, the four dosage forms occupy about 50 percent of market share, the four preparations are harmful to users and pollute the environment, the duration is short, and the residue and the resistance are easy to increase after a large amount of long-term use. Along with the continuous increase of environmental protection requirements, the water-based, granular, slow-release and multifunctional dosage forms with high efficiency, low toxicity, economy and environmental protection become the development direction and research hot spot of pesticide preparation processing.
Patent CN115005215a discloses a mixture and composition comprising a paenibacillus strain or fumonisins and a chemical pesticide which is effective against pests and pathogens but which has pesticide residues and negative environmental impact. Patent CN115386018A discloses a biological pesticide and a preparation method thereof, the invention uses chitosan in the five-cereal insects as a main raw material of the biological pesticide, can play a role in killing germs of crops, has less environmental pollution, but the product stability of the technology needs to be further improved. Patent CN115380899a discloses a gel-type humic acid slow-release pesticide and a preparation method thereof, cyclodextrin is taken as a reaction main body, chloroacetic acid is adopted to modify the cyclodextrin, carboxyl is introduced into the cyclodextrin to obtain modified cyclodextrin, then amino in the pesticide reacts with carboxyl in the modified cyclodextrin, an amide bond is generated by the reaction, and the amide is hydrolyzed when in use, so that the effective components of the pesticide are released, the effect of slow-release pesticide can be achieved, but the control effect of the pesticide needs to be further improved.
Therefore, in order to meet the demands of practical application, it is necessary to provide a novel pesticide which is friendly to the environment, low in residue, good in stability and good in insect pest prevention effect.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a novel biological pesticide which is friendly to the environment, low in residue, good in stability and good in control effect, so as to meet the requirements of practical application and environmental protection.
To achieve the purpose, the technical scheme of the invention is as follows:
the invention provides a novel biological pesticide, which comprises the following raw materials in parts by weight: 10-20 parts of antibacterial mixed solution, 20-30 parts of plant growth regulator, 12-18 parts of stramonium extract, 8-12 parts of rosemary extract, 6-10 parts of lemon thyme extract, 6-10 parts of negundo chastetree extract, 10-15 parts of pyrethrum, 10-20 parts of perilla essential oil, 10-15 parts of wintergreen plant essential oil, 5-10 parts of methyl caffeate, 5-10 parts of 6, 7-dihydroxycoumarin and 10-15 parts of surfactant.
In some embodiments of the invention, the antimicrobial cocktail is a cocktail of raffinose, luteolin, and matrine solution, the volume ratio of raffinose, matrine solution, and luteolin being 2-3:3-5:1-3 (e.g., can be 2:3:1, 2:4:1, 2:5:1, 2:3:2, 2:4:2, 2:5:2, 2:3:3, 2:4:3, 2:5:3, 3:3:1, 3:4:1, 3:3:2, 3:4:2, 3:5:2, 3:3:3:3, 3:4:3, 3:5:3, or any value therein).
In some embodiments of the invention, the method of preparing luteolin comprises the steps of:
(1) Inoculating Pseudomonas strain on a flat plate of a culture medium for activating growth, continuously selecting bacterial colony, and continuously carrying out passage on the bacterial colony on a glycerol culture medium for 3-5 times to obtain Pseudomonas strain for fermentation for later use;
(2) Inoculating the pseudomonas strain obtained in the step (1) into a glycerol-containing culture solution for growth, fermenting at constant temperature to obtain a fermentation liquor, filtering, loading the fermentation liquor onto macroporous resin, eluting with 100% methanol, collecting the eluent, and concentrating under reduced pressure at 60-70 ℃ to obtain the gamboge green bacteriocin.
In the preparation method of the tenuifolin, the culture medium and the culture solution comprise the following raw materials in parts by weight: 15-20 parts of glycerol, 5-10 parts of magnesium sulfate, 3-5 parts of monopotassium phosphate, 15-20 parts of peptone and 20-30 parts of water.
In the method for producing a ampelopsis grossedentata of the present invention, the fermentation temperature in the step (2) is 25 to 30 ℃ (for example, 25 ℃, 26 ℃, 27 ℃, 28 ℃, 29 ℃, 30 ℃ or any value thereof), and the fermentation time is 5 to 10 days (for example, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days or any value thereof).
In some embodiments of the invention, the matrine solution has a concentration of 0.5-1.5% (e.g., may be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5% or any value therein).
In some embodiments of the invention, the plant growth regulator is a mixture of flumetralin, mepiquat chloride, and prosaposin, the mass ratio of flumetralin, mepiquat chloride, and prosaposin being 1-2:1-2:4-12 (e.g., 1:1:4, 1:1:8, 1:1:12, 1:2:4, 1:2:8, 1:2:12, 2:1:4, 2:1:8, 2:1:12, or any value therein).
In some embodiments of the invention, the surfactant is selected from sodium dodecyl sulfate or polysorbate.
A method for preparing a novel biopesticide, the method comprising the steps of:
(1) Mixing herba Daturae extract, herba Rosmarini officinalis extract, herba Cymbopogonis Citrari extract, fructus Viticis negundo extract, herba et flos Pyrethri Cinerariifolii, perilla essential oil and herba Ilicis Purpureae essential oil, and stirring;
(2) Adding an antibacterial mixed solution and a plant growth regulator into the solution obtained in the step (1);
(3) And (3) adding methyl caffeate, 6, 7-dihydroxycoumarin and a surfactant into the solution obtained in the step (2), and continuously stirring to obtain the novel biological pesticide.
The application of the novel biological pesticide in preventing and controlling plant diseases comprises at least one of downy mildew, powdery mildew, gray mold, anthracnose, alternaria leaf spot, scab, rot, ring spot, canker, bacterial angular leaf spot, false smut, rice blast, banded sclerotial blight, bacterial stripe, stripe blight and virus diseases.
In some embodiments of the present invention, the novel biopesticide is applied to control plant diseases by: the novel biological pesticide is mixed with water for dilution, stirred uniformly and then sprayed in the morning or evening.
In the method of using the novel biopesticide of the present invention, the novel biopesticide is diluted at the time of use to a concentration of 3 to 6% (for example, 3%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6% or any value thereof), and the amount of the spray applied is 50 to 250kg/1000m 2 (e.g., 50kg/1000 m) 2 、70kg/1000m 2 、90kg/1000m 2 、110kg/1000m 2 、130kg/1000m 2 、150kg/1000m 2 、170kg/1000m 2 、190kg/1000m 2 、210kg/1000m 2 、230kg/1000m 2 、250kg/1000m 2 Or any value therein).
Compared with the prior art, the invention has the following beneficial effects:
(1) The novel biological pesticide provided by the invention can effectively inhibit the growth and propagation of bacteria and fungi through the collocation of matrine solution and the tenuifolia fungus, and the addition of a proper amount of raffinose can improve the stability of the tenuifolia fungus, prolong the half-life period of the matrine solution and the tenuifolia fungus, thereby enhancing the capability of preventing and controlling bacteria and insect pests.
(2) The novel biological pesticide provided by the invention uses the plant growth regulator which not only can regulate the function of an enzyme system in a plant body and increase the content of chlorophyll and protein and promote the reproductive growth of the plant, but also can be used together with the methyl caffeate and the 6, 7-dihydroxycoumarin, thereby effectively improving the stability of the pesticide.
(3) The novel biological pesticide provided by the invention has the advantages that the used plant extracts such as the perilla essential oil, the wintergreen plant essential oil, the stramonium extract, the rosemary extract and the lemon thyme extract are rich in plant polyphenol substances and flavonoid compounds, can show strong antioxidant activity, enzyme inhibition property and antibacterial property, have good antibacterial activity, and are beneficial to enhancing the pest control effect of the pesticide.
(4) The novel biological pesticide provided by the invention has the advantages that the added raw materials are natural, pollution-free and low-toxicity and safe, and the prepared novel biological pesticide is free of residues and public hazards, and is a novel pesticide which is environment-friendly and has a wide application prospect.
Detailed Description
The invention will be described below in connection with specific embodiments. The following examples are illustrative of the present invention and are not intended to limit the present invention. Other combinations and various modifications within the spirit of the invention may be made without departing from the spirit or scope of the invention. In the examples below, the compounds and related reagents used are commercially available.
Preparation of vine yellow green fungus
Medium/broth feedstock: 20 parts of glycerol, 10 parts of magnesium sulfate, 5 parts of monopotassium phosphate, 20 parts of peptone and 30 parts of water.
The preparation method comprises the following steps:
(1) Inoculating pseudomonas strains on a flat plate of a culture medium for activating growth, continuously selecting bacterial colonies, and continuously carrying out passage on the bacterial colonies on a glycerol culture medium for 3 times to obtain pseudomonas strains for fermentation for later use;
(2) Inoculating the pseudomonas strain obtained in the step (1) into a glycerol-containing culture solution for growth, fermenting for 5 days at 25 ℃ to obtain a fermentation liquor, filtering, loading the fermentation liquor onto macroporous resin, eluting with 100% methanol, collecting an eluent, and concentrating under reduced pressure at 60 ℃ to obtain the gamboge green bacteriocin.
Example 1
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 20 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=3:5:3), 30 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=2:1:12), 18 parts of stramonium extract, 12 parts of rosemary extract, 10 parts of lemon thyme extract, 10 parts of negundo chastetree extract, 15 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method comprises the following steps:
(1) Mixing herba Daturae extract, herba Rosmarini officinalis extract, herba Cymbopogonis Citrari extract, fructus Viticis negundo extract, herba et flos Pyrethri Cinerariifolii, perilla essential oil and herba Ilicis Purpureae essential oil, and stirring;
(2) Adding an antibacterial mixed solution and a plant growth regulator into the solution obtained in the step (1);
(3) And (3) adding methyl caffeate, 6, 7-dihydroxycoumarin and sodium dodecyl sulfate into the solution obtained in the step (2), and continuously stirring to obtain the novel biological pesticide.
Example 2
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 20 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 30 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=2:1:12), 18 parts of stramonium extract, 12 parts of rosemary extract, 10 parts of lemon thyme extract, 10 parts of negundo chastetree extract, 15 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
Example 3
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 30 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=2:1:12), 18 parts of stramonium extract, 12 parts of rosemary extract, 10 parts of lemon thyme extract, 10 parts of negundo chastetree extract, 15 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 4
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 30 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 18 parts of stramonium extract, 12 parts of rosemary extract, 10 parts of lemon thyme extract, 10 parts of negundo chastetree extract, 15 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 5
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 18 parts of stramonium extract, 12 parts of rosemary extract, 10 parts of lemon thyme extract, 10 parts of negundo chastetree extract, 15 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 6
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 12 parts of rosemary extract, 10 parts of lemon thyme extract, 10 parts of negundo chastetree extract, 15 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 7
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 8 parts of rosemary extract, 10 parts of lemon thyme extract, 10 parts of negundo chastetree extract, 15 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 8
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 8 parts of rosemary extract, 6 parts of lemon thyme extract, 10 parts of negundo chastetree extract, 15 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 9
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 8 parts of rosemary extract, 6 parts of lemon thyme extract, 6 parts of negundo chastetree extract, 15 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 10
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 8 parts of rosemary extract, 6 parts of lemon thyme extract, 6 parts of negundo chastetree extract, 10 parts of pyrethrum, 20 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 11
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 8 parts of rosemary extract, 6 parts of lemon thyme extract, 6 parts of negundo chastetree extract, 10 parts of pyrethrum, 10 parts of perilla essential oil, 15 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 12
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 8 parts of rosemary extract, 6 parts of lemon thyme extract, 6 parts of negundo chastetree extract, 10 parts of pyrethrum, 10 parts of perilla essential oil, 10 parts of wintergreen plant essential oil, 10 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 13
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 8 parts of rosemary extract, 6 parts of lemon thyme extract, 6 parts of negundo chastetree extract, 10 parts of pyrethrum, 10 parts of perilla essential oil, 10 parts of wintergreen plant essential oil, 5 parts of methyl caffeoate, 10 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 14
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 8 parts of rosemary extract, 6 parts of lemon thyme extract, 6 parts of negundo chastetree extract, 10 parts of pyrethrum, 10 parts of perilla essential oil, 10 parts of wintergreen plant essential oil, 5 parts of methyl caffeoate, 5 parts of 6, 7-dihydroxycoumarin and 15 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Example 15
The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10 parts of antibacterial mixed solution (raffinose, vine yellow green fungus extract, matrine solution=2:3:1), 20 parts of plant growth regulator (flumetralin, mepiquat chloride, thiomerone=1:1:4), 12 parts of stramonium extract, 8 parts of rosemary extract, 6 parts of lemon thyme extract, 6 parts of negundo chastetree extract, 10 parts of pyrethrum, 10 parts of perilla essential oil, 10 parts of wintergreen plant essential oil, 5 parts of methyl caffeoate, 5 parts of 6, 7-dihydroxycoumarin and 10 parts of sodium dodecyl sulfate.
The preparation method is the same as in example 1.
Comparative example 1
The difference from example 1 is that: no antibacterial mixed solution is added.
Comparative example 2
The difference from example 1 is that: raffinose is absent from the added antimicrobial blend.
Comparative example 3
The difference from example 1 is that: the added antibacterial mixed solution lacks the ampelopsin.
Comparative example 4
The difference from example 1 is that: no plant growth regulator was added.
Comparative example 5
The difference from example 1 is that: no 6, 7-dihydroxycoumarin is added.
Comparative example 6
The difference from example 1 is that: no addition of methyl caffeate and 6, 7-dihydroxycoumarin was added.
Taking the novel biopesticides prepared in the examples and the comparative examples, adding water to dilute the concentration to 5%, and spraying the mixture to the volume of 150kg/1000m 2 Spraying the crops. Counting the diseased plants of each sample after 15 days, calculating to obtain the control efficiency of insect-preventing and pest-preventing pesticides, and detecting the residue of novel biological pesticides in crops after 28 days; the heat storage stability of the novel biological pesticide is measured according to the method in the national standard of the people's republic of China, the method for measuring the heat storage stability of pesticides, GB/T19136-2003; the cold storage stability is measured according to the method in national Standard of the people's republic of China, method for measuring Low temperature stability of pesticides, GB/T19137-2003, and the measurement results are shown in Table 1.
Table 1 results of test of the performance of novel biopesticide of examples and comparative examples
Numbering device Control rate (%) Residual Rate (%) Thermal storage stability Stability to cold storage
Example 1 94 0 Qualified product Qualified product
Example 2 93 0 Qualified product Qualified product
Example 3 93 0 Qualified product Qualified product
Example 4 92 0 Qualified product Qualified product
Example 5 92 0 Qualified product Qualified product
Example 6 92 0 Qualified product Qualified product
Example 7 91 0 Qualified product Qualified product
Example 8 91 0 Qualified product Qualified product
Example 9 91 0 Qualified product Qualified product
Example 10 90 0 Qualified product Qualified product
Example 11 90 0 Qualified product Qualified product
Example 12 90 0 Qualified product Qualified product
Example 13 89 0 Qualified product Qualified product
Example 14 89 0 Qualified product Qualified product
Example 15 89 0 Qualified product Qualified product
Comparative example 1 74 0 Qualified product Qualified product
Comparative example 2 78 0 Qualified product Qualified product
Comparative example 3 76 0 Qualified product Qualified product
Comparative example 4 88 0 Failure to pass Failure to pass
Comparative example 5 86 0 Failure to pass Failure to pass
Comparative example 6 83 0 Failure to pass Failure to pass
As can be seen from comparison of the test results of comparative examples 1, 2 and 3 and example 1, the novel biopesticide prepared in example 1 has higher control rate and can effectively prevent insect pests; from comparison of comparative examples 4, 5 and 6 with example 1, it is apparent that the stability of the novel biopesticide can be remarkably improved by adding a plant growth regulator, methyl caffeate and 6, 7-dihydroxycoumarin. As can be seen from comprehensive comparison of test results of examples and comparative examples, the novel biopesticide provided by the invention can effectively inhibit growth and reproduction of bacteria and fungi by adding the antibacterial mixed solution, and the addition of a proper amount of raffinose can improve the stability of the ampelopsin and prolong the half-life period of matrine solution and the ampelopsin, so that the capability of preventing and controlling bacterial and insect pests is enhanced.
The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the present invention and to implement it, but not limit the scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be included in the scope of the present invention.

Claims (10)

1. The novel biological pesticide is characterized by comprising the following raw materials in parts by weight: 10-20 parts of antibacterial mixed solution, 20-30 parts of plant growth regulator, 12-18 parts of stramonium extract, 8-12 parts of rosemary extract, 6-10 parts of lemon thyme extract, 6-10 parts of negundo chastetree extract, 10-15 parts of pyrethrum, 10-20 parts of perilla essential oil, 10-15 parts of wintergreen plant essential oil, 5-10 parts of methyl caffeate, 5-10 parts of 6, 7-dihydroxycoumarin and 10-15 parts of surfactant.
2. The novel biopesticide according to claim 1, wherein the antibacterial mixed liquid is a mixed liquid of raffinose, gamboge green fungus extract and matrine solution, and the volume ratio of raffinose, matrine solution and gamboge green fungus extract is 2-3:3-5:1-3.
3. The novel biopesticide according to claim 2, wherein the method for producing gamboge green fungus extract comprises the steps of:
(1) Inoculating Pseudomonas strain on a flat plate of a culture medium for activating growth, continuously selecting bacterial colony, and continuously carrying out passage on the bacterial colony on a glycerol culture medium for 3-5 times to obtain Pseudomonas strain for fermentation for later use;
(2) Inoculating the pseudomonas strain obtained in the step (1) into a glycerol-containing culture solution for growth, fermenting at constant temperature to obtain a fermentation liquor, filtering, loading the fermentation liquor into macroporous resin, eluting with 100% methanol, collecting the eluent, and concentrating under reduced pressure at 60-70 ℃ to obtain the product, namely the gamboge green bacteriocin.
4. The novel biopesticide according to claim 3, wherein the medium and the culture liquid comprise the following raw materials in parts by weight: 15-20 parts of glycerol, 5-10 parts of magnesium sulfate, 3-5 parts of monopotassium phosphate, 15-20 parts of peptone and 20-30 parts of water; the fermentation temperature in the step (2) is 25-30 ℃, and the fermentation time is 5-10 days.
5. The novel biopesticide according to claim 2, wherein the concentration of the matrine solution is 0.5-1.5%.
6. The novel biopesticide according to claim 1, wherein the plant growth regulator is a mixture of flumetralin, mepiquat chloride and budding inhibitor, and the mass ratio of flumetralin, mepiquat chloride and budding inhibitor is 1-2:1-2:4-12.
7. The novel biopesticide according to claim 1, wherein the surfactant is selected from sodium dodecyl sulfate or polysorbate.
8. A method for preparing a novel biopesticide according to any one of claims 1 to 7, characterized in that the novel biopesticide preparation method comprises the steps of:
(1) Mixing herba Daturae extract, herba Rosmarini officinalis extract, herba Cymbopogonis Citrari extract, fructus Viticis negundo extract, herba et flos Pyrethri Cinerariifolii, perilla essential oil and herba Ilicis Purpureae essential oil, and stirring;
(2) Adding an antibacterial mixed solution and a plant growth regulator into the solution obtained in the step (1);
(3) And (3) adding methyl caffeate, 6, 7-dihydroxycoumarin and a surfactant into the solution obtained in the step (2), and continuously stirring to obtain the novel biological pesticide.
9. The use of a novel biopesticide according to any one of claims 1 to 7 for controlling plant diseases, wherein the plant diseases include at least one of downy mildew, powdery mildew, gray mold, anthracnose, alternaria leaf spot, scab, rot, ring spot, canker, bacterial angular leaf spot, false smut, rice blast, banded sclerotial blight, bacterial stripe, stripe leaf blight and virus diseases.
10. Use of the novel biopesticide according to claim 9 in the field of controlling plant diseasesThe application method of the novel biological pesticide in preventing and controlling plant diseases is characterized in that: mixing the novel biological pesticide with water, diluting to 3-6%, stirring, and spraying in the morning or evening at 50-250kg/1000m 2 。
CN202310052556.4A 2023-02-02 2023-02-02 Novel biological pesticide and preparation method and application thereof Pending CN116268009A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104041546A (en) * 2014-05-29 2014-09-17 青岛辰青信息技术有限公司 Insecticide
CN105309487A (en) * 2014-06-24 2016-02-10 陕西美邦农药有限公司 High-effective pesticide composition containing polygonum cuspidatum extract
CN108552267A (en) * 2018-05-16 2018-09-21 芜湖扬展新材料科技服务有限公司 A kind of plant compound pesticide composition and preparation method thereof
CN108782624A (en) * 2018-04-28 2018-11-13 芜湖扬展新材料科技服务有限公司 A kind of agricultural chemical of natural plant

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104041546A (en) * 2014-05-29 2014-09-17 青岛辰青信息技术有限公司 Insecticide
CN105309487A (en) * 2014-06-24 2016-02-10 陕西美邦农药有限公司 High-effective pesticide composition containing polygonum cuspidatum extract
CN108782624A (en) * 2018-04-28 2018-11-13 芜湖扬展新材料科技服务有限公司 A kind of agricultural chemical of natural plant
CN108552267A (en) * 2018-05-16 2018-09-21 芜湖扬展新材料科技服务有限公司 A kind of plant compound pesticide composition and preparation method thereof

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