CN116157517A - 目标dna的编辑方法、编辑了目标dna的细胞的制备方法及用于它们的dna编辑系统 - Google Patents

目标dna的编辑方法、编辑了目标dna的细胞的制备方法及用于它们的dna编辑系统 Download PDF

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CN116157517A
CN116157517A CN202180054310.XA CN202180054310A CN116157517A CN 116157517 A CN116157517 A CN 116157517A CN 202180054310 A CN202180054310 A CN 202180054310A CN 116157517 A CN116157517 A CN 116157517A
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佐久间哲史
山本卓
西堀奈穗子
吉间忠彦
堀込一彦
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Hiroshima University NUC
Sumitomo Chemical Co Ltd
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Hiroshima University NUC
Sumitomo Chemical Co Ltd
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202180054310.XA 2020-09-04 2021-09-03 目标dna的编辑方法、编辑了目标dna的细胞的制备方法及用于它们的dna编辑系统 Pending CN116157517A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020148944 2020-09-04
JP2020-148944 2020-09-04
PCT/JP2021/032436 WO2022050377A1 (ja) 2020-09-04 2021-09-03 標的ｄｎａの編集方法、標的ｄｎａが編集された細胞の製造方法、及びそれらに用いるｄｎａ編集システム

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CN116157517A true CN116157517A (zh) 2023-05-23

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CN202180054310.XA Pending CN116157517A (zh) 2020-09-04 2021-09-03 目标dna的编辑方法、编辑了目标dna的细胞的制备方法及用于它们的dna编辑系统

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US (1) US20230340455A1 (https=)
EP (1) EP4209588A4 (https=)
JP (1) JPWO2022050377A1 (https=)
KR (1) KR20230058482A (https=)
CN (1) CN116157517A (https=)
AU (1) AU2021338062A1 (https=)
WO (1) WO2022050377A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2024228481A1 (en) 2023-02-28 2025-08-28 Sumitomo Chemical Company, Limited Nicking enzyme, dna editing system, target dna editing method, and method for producing cell in which target dna is edited
CN119876242A (zh) * 2025-02-12 2025-04-25 华中农业大学 一种碱基编辑系统GhDA3A-BE4与碱基编辑方法及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018218206A1 (en) * 2017-05-25 2018-11-29 The General Hospital Corporation Bipartite base editor (bbe) architectures and type-ii-c-cas9 zinc finger editing
CN109804066A (zh) * 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
WO2020051562A2 (en) * 2018-09-07 2020-03-12 Beam Therapeutics Inc. Compositions and methods for improving base editing

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150166969A1 (en) * 2012-02-24 2015-06-18 Fred Hutchinson Cancer Research Center Compositions and methods for the treatment of hemoglobinopathies
EP2922393B2 (en) 2013-02-27 2022-12-28 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Gene editing in the oocyte by cas9 nucleases
IL310721B2 (en) * 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109804066A (zh) * 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
WO2018218206A1 (en) * 2017-05-25 2018-11-29 The General Hospital Corporation Bipartite base editor (bbe) architectures and type-ii-c-cas9 zinc finger editing
WO2020051562A2 (en) * 2018-09-07 2020-03-12 Beam Therapeutics Inc. Compositions and methods for improving base editing

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Publication number Publication date
EP4209588A4 (en) 2024-06-26
AU2021338062A1 (en) 2023-05-04
JPWO2022050377A1 (https=) 2022-03-10
KR20230058482A (ko) 2023-05-03
EP4209588A1 (en) 2023-07-12
US20230340455A1 (en) 2023-10-26
WO2022050377A1 (ja) 2022-03-10

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