KR20230058482A - 표적 dna의 편집 방법, 표적 dna가 편집된 세포의 제조 방법, 및 그것들에 사용하는 dna 편집 시스템 - Google Patents

표적 dna의 편집 방법, 표적 dna가 편집된 세포의 제조 방법, 및 그것들에 사용하는 dna 편집 시스템 Download PDF

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KR20230058482A
KR20230058482A KR1020237010913A KR20237010913A KR20230058482A KR 20230058482 A KR20230058482 A KR 20230058482A KR 1020237010913 A KR1020237010913 A KR 1020237010913A KR 20237010913 A KR20237010913 A KR 20237010913A KR 20230058482 A KR20230058482 A KR 20230058482A
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데츠시 사쿠마
다카시 야마모토
나호코 니시보리
다다히코 요시마
가즈히코 호리고메
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고쿠리츠다이가쿠호진 히로시마다이가쿠
스미또모 가가꾸 가부시키가이샤
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Enzymes And Modification Thereof (AREA)
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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020237010913A 2020-09-04 2021-09-03 표적 dna의 편집 방법, 표적 dna가 편집된 세포의 제조 방법, 및 그것들에 사용하는 dna 편집 시스템 Pending KR20230058482A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020148944 2020-09-04
JPJP-P-2020-148944 2020-09-04
PCT/JP2021/032436 WO2022050377A1 (ja) 2020-09-04 2021-09-03 標的ｄｎａの編集方法、標的ｄｎａが編集された細胞の製造方法、及びそれらに用いるｄｎａ編集システム

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KR20230058482A true KR20230058482A (ko) 2023-05-03

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KR1020237010913A Pending KR20230058482A (ko) 2020-09-04 2021-09-03 표적 dna의 편집 방법, 표적 dna가 편집된 세포의 제조 방법, 및 그것들에 사용하는 dna 편집 시스템

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US (1) US20230340455A1 (https=)
EP (1) EP4209588A4 (https=)
JP (1) JPWO2022050377A1 (https=)
KR (1) KR20230058482A (https=)
CN (1) CN116157517A (https=)
AU (1) AU2021338062A1 (https=)
WO (1) WO2022050377A1 (https=)

Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
AU2024228481A1 (en) 2023-02-28 2025-08-28 Sumitomo Chemical Company, Limited Nicking enzyme, dna editing system, target dna editing method, and method for producing cell in which target dna is edited
CN119876242A (zh) * 2025-02-12 2025-04-25 华中农业大学 一种碱基编辑系统GhDA3A-BE4与碱基编辑方法及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018218166A1 (en) 2017-05-25 2018-11-29 The General Hospital Corporation Using split deaminases to limit unwanted off-target base editor deamination

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Publication number Priority date Publication date Assignee Title
US20150166969A1 (en) * 2012-02-24 2015-06-18 Fred Hutchinson Cancer Research Center Compositions and methods for the treatment of hemoglobinopathies
EP2922393B2 (en) 2013-02-27 2022-12-28 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Gene editing in the oocyte by cas9 nucleases
IL310721B2 (en) * 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
WO2018031683A1 (en) * 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2020051562A2 (en) * 2018-09-07 2020-03-12 Beam Therapeutics Inc. Compositions and methods for improving base editing

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018218166A1 (en) 2017-05-25 2018-11-29 The General Hospital Corporation Using split deaminases to limit unwanted off-target base editor deamination

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
Gaudelli, N. et al., Nature 551, 464-471(2017)
Gehrke, J. et al., Nat Biotechnol 36, 977-982(2018)
Koblan, L. et al., Nat Biotechnol 36, 843-846(2018)
Komor, A. et al., Nature 533, 420-424(2016)
Martin, A. S. et al., Sci Rep 9, 497(2019)
Nishida, K. et al., Science 353, aaf8729(2016)
Rees, H. A. and Liu, D. R., Nat Rev Genet 19, 770-788(2018)
Yang, L. et al., Nat Commun 7, 13330(2016)

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EP4209588A4 (en) 2024-06-26
AU2021338062A1 (en) 2023-05-04
JPWO2022050377A1 (https=) 2022-03-10
EP4209588A1 (en) 2023-07-12
US20230340455A1 (en) 2023-10-26
WO2022050377A1 (ja) 2022-03-10
CN116157517A (zh) 2023-05-23

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