CN116157510A - 用于制造多能干细胞的材料和方法 - Google Patents

用于制造多能干细胞的材料和方法 Download PDF

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CN116157510A
CN116157510A CN202180055632.6A CN202180055632A CN116157510A CN 116157510 A CN116157510 A CN 116157510A CN 202180055632 A CN202180055632 A CN 202180055632A CN 116157510 A CN116157510 A CN 116157510A
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cells
cell
isolated population
ipscs
days
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I·S·格雷沃
R·甘尼桑
S·辛格
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Janssen Biotech Inc
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CN202180055632.6A 2020-06-17 2021-06-16 用于制造多能干细胞的材料和方法 Pending CN116157510A (zh)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US202063040398P 2020-06-17 2020-06-17
US202063040392P 2020-06-17 2020-06-17
US202063040373P 2020-06-17 2020-06-17
US202063040374P 2020-06-17 2020-06-17
US202063040397P 2020-06-17 2020-06-17
US63/040398 2020-06-17
US63/040373 2020-06-17
US63/040397 2020-06-17
US63/040392 2020-06-17
US63/040374 2020-06-17
PCT/US2021/037594 WO2021257679A1 (en) 2020-06-17 2021-06-16 Materials and methods for the manufacture of pluripotent stem cells

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CN116157510A true CN116157510A (zh) 2023-05-23

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US (2) US11981932B2 (https=)
EP (1) EP4168433A4 (https=)
JP (1) JP2023530919A (https=)
KR (1) KR20230025005A (https=)
CN (1) CN116157510A (https=)
AU (1) AU2021292511A1 (https=)
CA (1) CA3187267A1 (https=)
IL (1) IL298909A (https=)
TW (1) TW202214844A (https=)
WO (1) WO2021257679A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7224021B2 (ja) * 2017-02-03 2023-02-17 国立大学法人神戸大学 人工多能性幹細胞の作製方法
CA3203232A1 (en) * 2020-12-25 2022-06-30 Kyoto University Method for producing naive human ips cells from somatic cells
IL318041A (en) 2022-07-01 2025-02-01 Janssen Biotech Inc Materials and methods for bioengineered iPSC populations
US20260071238A1 (en) 2022-09-02 2026-03-12 Janssen Biotech, Inc. Materials and processes for engineering hypoimmunogenicity
WO2024127335A1 (en) * 2022-12-15 2024-06-20 Janssen Biotech, Inc. Materials, methods and systems for cellular redifferentiation and expansion

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106459918A (zh) * 2014-04-24 2017-02-22 得克萨斯州大学系统董事会 施加诱导多能干细胞产生过继细胞疗法产品
CN106471118A (zh) * 2014-03-11 2017-03-01 新加坡科技研究局 在造血细胞中诱导多能性的方法
US20180170982A1 (en) * 2010-10-22 2018-06-21 Biotime, Inc. Methods of modifying transcriptional regulatory networks in stem cells
US20200017837A1 (en) * 2017-02-03 2020-01-16 National University Corporation Kobe University Method For Producing Induced Pluripotent Stem Cells

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0092918B1 (en) 1982-04-22 1988-10-19 Imperial Chemical Industries Plc Continuous release formulations
IL68218A (en) 1983-03-23 1985-12-31 Univ Ramot Compositions for cartilage repair comprising embryonal chondrocytes
US5128326A (en) 1984-12-06 1992-07-07 Biomatrix, Inc. Drug delivery systems based on hyaluronans derivatives thereof and their salts and methods of producing same
US4690915A (en) 1985-08-08 1987-09-01 The United States Of America As Represented By The Department Of Health And Human Services Adoptive immunotherapy as a treatment modality in humans
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5041138A (en) 1986-11-20 1991-08-20 Massachusetts Institute Of Technology Neomorphogenesis of cartilage in vivo from cell culture
US4846835A (en) 1987-06-15 1989-07-11 Grande Daniel A Technique for healing lesions in cartilage
EP0368684B2 (en) 1988-11-11 2004-09-29 Medical Research Council Cloning immunoglobulin variable domain sequences.
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
AU6430190A (en) 1989-10-10 1991-05-16 Pitman-Moore, Inc. Sustained release composition for macromolecular proteins
CA2071867A1 (en) 1989-11-06 1991-05-07 Edith Mathiowitz Method for producing protein microspheres
GB8928874D0 (en) 1989-12-21 1990-02-28 Celltech Ltd Humanised antibodies
EP0519596B1 (en) 1991-05-17 2005-02-23 Merck & Co. Inc. A method for reducing the immunogenicity of antibody variable domains
EP1400536A1 (en) 1991-06-14 2004-03-24 Genentech Inc. Method for making humanized antibodies
US5565332A (en) 1991-09-23 1996-10-15 Medical Research Council Production of chimeric antibodies - a combinatorial approach
US5869619A (en) 1991-12-13 1999-02-09 Xoma Corporation Modified antibody variable domains
EP0571613B1 (en) 1991-12-13 2003-09-17 Xoma Corporation Methods and materials for preparation of modified antibody variable domains and therapeutic uses thereof
GB9203459D0 (en) 1992-02-19 1992-04-08 Scotgen Ltd Antibodies with germ-line variable regions
US5912015A (en) 1992-03-12 1999-06-15 Alkermes Controlled Therapeutics, Inc. Modulated release from biocompatible polymers
IL104570A0 (en) 1992-03-18 1993-05-13 Yeda Res & Dev Chimeric genes and cells transformed therewith
DK1621554T4 (da) 1992-08-21 2012-12-17 Univ Bruxelles Immunoglobuliner blottet for lette kæder
US5639641A (en) 1992-09-09 1997-06-17 Immunogen Inc. Resurfacing of rodent antibodies
US5646008A (en) 1993-06-22 1997-07-08 The Regent Of The University Of Michigan Vertebrate apoptosis gene: compositions and methods
US5723331A (en) 1994-05-05 1998-03-03 Genzyme Corporation Methods and compositions for the repair of articular cartilage defects in mammals
US5698446A (en) 1994-09-07 1997-12-16 Chiron Corporation Methods and compositions for inhibiting production of replication competent virus
ATE252894T1 (de) 1995-01-05 2003-11-15 Univ Michigan Oberflächen-modifizierte nanopartikel und verfahren für ihre herstellung und verwendung
EP0739981A1 (en) 1995-04-25 1996-10-30 Vrije Universiteit Brussel Variable fragments of immunoglobulins - use for therapeutic or veterinary purposes
WO1997007788A2 (en) 1995-08-31 1997-03-06 Alkermes Controlled Therapeutics, Inc. Composition for sustained release of an agent
US5989463A (en) 1997-09-24 1999-11-23 Alkermes Controlled Therapeutics, Inc. Methods for fabricating polymer-based controlled release devices
SE512663C2 (sv) 1997-10-23 2000-04-17 Biogram Ab Inkapslingsförfarande för aktiv substans i en bionedbrytbar polymer
AU3596599A (en) 1998-01-26 1999-08-09 Unilever Plc Method for producing antibody fragments
AU2474700A (en) 1998-12-01 2000-06-19 Genentech Inc. Secreted amd transmembrane polypeptides and nucleic acids encoding the same
DK1144616T4 (da) 1999-01-19 2009-03-30 Unilever Nv Fremgangsmåde til fremstilling af antistoffragmenter
WO2000063374A1 (en) 1999-04-16 2000-10-26 Celltech Therapeutics Limited Synthetic transmembrane components
US20030190598A1 (en) 2000-05-26 2003-10-09 Jasmid Tanha Single-domain antigen-binding antibody fragments derived from llama antibodies
US20060073141A1 (en) 2001-06-28 2006-04-06 Domantis Limited Compositions and methods for treating inflammatory disorders
PT1419179E (pt) 2001-08-10 2010-03-16 Univ Aberdeen Domínios de ligação de antigenes
JP4213586B2 (ja) 2001-09-13 2009-01-21 株式会社抗体研究所 ラクダ抗体ライブラリーの作製方法
JP2005289809A (ja) 2001-10-24 2005-10-20 Vlaams Interuniversitair Inst Voor Biotechnologie Vzw (Vib Vzw) 突然変異重鎖抗体
US20030170238A1 (en) 2002-03-07 2003-09-11 Gruenberg Micheal L. Re-activated T-cells for adoptive immunotherapy
GB0228210D0 (en) 2002-12-03 2003-01-08 Babraham Inst Single chain antibodies
WO2005035575A2 (en) 2003-08-22 2005-04-21 Medimmune, Inc. Humanization of antibodies
US7563443B2 (en) 2004-09-17 2009-07-21 Domantis Limited Monovalent anti-CD40L antibody polypeptides and compositions thereof
AU2007219159B8 (en) 2006-01-25 2012-06-28 Roger Kingdon Craig Generation of heavy-chain only antibodies in transgenic animals
US20100122358A1 (en) 2008-06-06 2010-05-13 Crescendo Biologics Limited H-Chain-only antibodies
ES2724975T3 (es) 2009-12-10 2019-09-18 Regeneron Pharma Ratones que producen anticuerpos de cadena pesada
RU2658485C2 (ru) 2012-10-24 2018-06-21 Дзе Юнайтед Стейтс Оф Америка, Эз Репрезентед Бай Дзе Секретари, Департмент Оф Хелс Энд Хьюман Сёрвисез Химерные антигенные рецепторы м971
CA3124228C (en) 2014-03-21 2024-05-14 Regeneron Pharmaceuticals, Inc. Non-human animals that make single domain binding proteins
EP3131927B8 (en) 2014-04-14 2020-12-23 Cellectis Bcma (cd269) specific chimeric antigen receptors for cancer immunotherapy
ES2878449T3 (es) 2014-07-24 2021-11-18 2Seventy Bio Inc Receptores antigénicos quiméricos de BCMA
PL3560953T3 (pl) 2014-12-24 2024-05-13 Autolus Limited Komórka
BR112019006781A2 (pt) 2016-10-07 2019-07-30 Novartis Ag receptores de antígeno quiméricos para o tratamento de câncer
EP3580332B1 (en) * 2017-02-07 2026-04-15 Agency for Science, Technology And Research Methods and kits for generating mimetic innate immune cells from pluripotent stem cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180170982A1 (en) * 2010-10-22 2018-06-21 Biotime, Inc. Methods of modifying transcriptional regulatory networks in stem cells
CN106471118A (zh) * 2014-03-11 2017-03-01 新加坡科技研究局 在造血细胞中诱导多能性的方法
CN106459918A (zh) * 2014-04-24 2017-02-22 得克萨斯州大学系统董事会 施加诱导多能干细胞产生过继细胞疗法产品
US20200017837A1 (en) * 2017-02-03 2020-01-16 National University Corporation Kobe University Method For Producing Induced Pluripotent Stem Cells

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